Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0011160

Physical Location & Seq

Genomic Coordinates Forward (+)

7710709 .. 7716023

Gene Structure 5315 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g13980.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGGCGGACACTCTGAGCGCTCTGAGGTCTTTAATGGCGTCTCACTCTCCTCCTCTTGACGCCTTGGTTGTTCCTTCCGAAGATTACCACCAGAGCGAGTATGTTTCCGCCAGTGACAAAAGACGTGAATTCGTTTCCGGCTTCACTGGAAGTGCCGGTTTGGGGCTTATAACGAAGAATGAAGCACGGCTCTGGACAGATGGCCGCTACTTTTTGCAGGCAACACAGCAACTTAGTGACCAGTGGCAGCTTATGCGAATGGGAGAAGATCCTGCTGTAGATGTCTGGATGGCTGATAATCTCCCTGAAGGTTCGGCCATTGGTATAGATCCTTGGTGTGTATCGATAGACACTGCACAGAAATGGGAGCGTGCTTTTGTCAAGAAACAACAGAAGTTGGTTCAAACTTCCATAAATTTGGTGGATGAAGTTTGGAAAAGTCAGCCCCCTCCTGAAATTAATCCTGTAGTTATTCATCCTTTGGAATATGCTGGTCGTTCTGTAGCAGATAAGTTGAAGGATGTGAGAAAAAAGCTCGTCCAAGTGAAAGCTCATAGTATAATCATCTCCGCACTTGATGAGGTTTGCTGGTTGTATAATATCCGTGGCACTGATGTTGCTTACAGTCCTGTTGTTCATGCCTTTGCCATAGTTACTCTGAACTCAGCGTTCTTCTATGTGGACAAAAGGAAGGTTTCATCCGAGGTAAACTCATACTTGGAAACAAACGGAATTGAAGTTCGGGACTATAAAGTTGTGAGCTCAGATGTCAGCTCGCTTGCATCTAATGAGCTTAAGCCTTCAATTCAAGCCAAGGGAACTGAAACTGAAACAGCAGGAAATGGTACAATAAAAGCTGAAGAAAGTAATAATGACCTCATATGGGCTGACCCTGGTTATTGTTGTTATGCATTGTATTCAAGGCTAAACCCTGACAAGGTTCTCTTGCAGCAGTCACCTTTGGCCCTTGCAAAAGCTCTGAAGAACCCAGTTGAGTTGGAAGGGTTAAAAAATGCTCACATTCGAGATGGTGCGGCTGTTGTGCAATATCATGTCTGGTTGGATAAACAGATGCAGGAGATGTACGGTGCCTCTGGTTTCTTTTTGGAGGAGGAAACAGATAAGAAAAAACACTCGAAAACTGCAAAACTAACTGAGGTGACTGCAAGCGATAAGTTGGAAGGCTTCCGAGCATCAAAAGAGAATTTTAGAGGATTAAGCTTCCCTACTATTTCCTCGGTTGGCCCAAATGCTGCCATTATCCATTATTCACCAGAAGCAGAGACCTGTGCTGAGTTGGATCCAGACCGCATATATCTTTTTGACTCTGGAGCACAGTATCTGGATGGAACAACTGACATAACACGAACAGTTCATTTTGGGAAACCTTCAACTCATGAGAAAGCATGCTATACCGCAGTCCTCAAAGGTCACATTGCTCTAGGGAATGCTCATTTTCCTAATGGAACCAATGGCCATGCCCTGGACATTCTGGCTCGAGTTCCTTTGTGGAGGTATGGTCTCGATTATCGACATGGTACTGGTCATGGGATTGGATCTTACCTAAATGTTCACGAAGGACCCCATCTAATTAGTTTCAGACCTCGCAATGTCCCACTGGAAGATTCTATGACTGTAACAGATGAACCTGGATATTATGAGGATGGGAATTTTGGTATAAGATTGGAGAACGTGCTTATAATCAAGAAAGCTGATACAAAATTCAATTTTGGTGACAAGGGCTACTTGCAATTTGAGCACATAACATGGGCACCCTATCAGAGGAAGTTAATTGATGTAAGTCTTTTAACACCCGAAGAGTTGGAATGGCTGAACACCTACCATTCCAAATGTAGATCTATTCTGGACTCGTACTTGGATGAATCTGAGAAGGCATGGCTGAAGAAAGCCACTGAACCCATCAGTGTGAGTTGGACTGAATGCCCTGAACGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006508 GO:0006807 GO:0006810 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008238 GO:0008270 GO:0009914 GO:0009926 GO:0010013 GO:0010817 GO:0016020 GO:0016787 GO:0019538 GO:0030145 GO:0031406 GO:0033218 GO:0033293 GO:0036094 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:0070011 GO:0071704 GO:0071944 GO:0097159 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Creatinase_N Creatinase_N_2 Peptidase_M24 Peptidase_M24_C

Protein Analysis

652

Amino Acids

72.86

Weight (kDa)

5.61

Isoelectric Point (pI)

33.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Creatinase_N	PF01321	6 - 137	1.3e-12	Creatinase/Prolidase N-terminal domain
Creatinase_N_2	PF16189	148 - 332	7.9e-47	Creatinase/Prolidase N-terminal domain
Peptidase_M24	PF00557	383 - 569	6.2e-48	Metallopeptidase family M24
Peptidase_M24_C	PF16188	581 - 641	2.1e-25	C-terminal region of peptidase_M24

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011160)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36760 AT4G36760 AT4G36760
fragaria_vesca	FvH4_1g13980
malus_domestica	MD02G1152800.v1.1 MD15G1267200.v1.1
prunus_persica	Prupe.7G149300_v2.0.a1
pyrus_communis	pycom02g12060 pycom15g23280
rosa_chinensis	RchiOBHm_Chr2g0102281
rosa_laevigata	RLG00000017142 RLG00000017146
rosa_multiflora	Rmu_sc0005055.1_g000022 Rmu_sc0030874.1_g000002
rosa_roxburghii	Rroxscaffold_2G00140610
rosa_rugosa	Rorug02G0106100
rosa_samantha	Rh2AG154100 Rh2CG159700 Rh2DG159700
rosa_wichuraiana	Rw2G012050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	170, 1700
AccB1I	GGYRCC	2 cut(s)	1088, 1771
AccB7I	CCANNNNNTGG	1 cut(s)	1483
AciI	CCGC	7 cut(s)	5, 108, 205, 570, 1034, 1309, 1416
AclWI	GGATC	5 cut(s)	263, 323, 1295, 1308, 1564
AcoI	YGGCCR	3 cut(s)	202, 315, 1474
AcsI	RAATTY	5 cut(s)	128, 415, 1204, 1669, 1721
AcuI	CTGAAG	4 cut(s)	327, 879, 1001, 1922
AcyI	GRCGYC	2 cut(s)	38, 60
AfaI	GTAC	4 cut(s)	847, 1085, 1540, 1874
AfeI	AGCGCT	1 cut(s)	19
AfiI	CCNNNNNNNGG	5 cut(s)	162, 883, 937, 1483, 1782
AflII	CTTAAG	1 cut(s)	794
AgsI	TTSAA	8 cut(s)	404, 517, 737, 804, 809, 921, 1392, 1726
AjiI	CACGTC	1 cut(s)	125
AjnI	CCWGG	3 cut(s)	892, 1482, 1648
AloI	GAACNNNNNNTCC	2 cut(s)	723, 755
Alw21I	GWGCWC	3 cut(s)	764, 1336, 1761
Alw26I	GTCTC	3 cut(s)	45, 1277, 1526
AlwI	GGATC	5 cut(s)	263, 323, 1295, 1308, 1564
AlwNI	CAGNNNCTG	1 cut(s)	1342
Ama87I	CYCGRG	1 cut(s)	1497
Aor51HI	AGCGCT	1 cut(s)	19
AoxI	GGCC	5 cut(s)	202, 315, 963, 1243, 1474
ApeKI	GCWGC	3 cut(s)	247, 949, 1253
ApoI	RAATTY	5 cut(s)	128, 415, 1204, 1669, 1721
AseI	ATTAAT	1 cut(s)	459
AspLEI	GCGC	1 cut(s)	20
AspS9I	GGNCC	3 cut(s)	964, 1244, 1580
AsuHPI	GGTGA	4 cut(s)	948, 1171, 1263, 1745
AvaI	CYCGRG	1 cut(s)	1497
AvaII	GGWCC	1 cut(s)	1580
BaeGI	GKGCMC	1 cut(s)	1774
BalI	TGGCCA	1 cut(s)	1476
BamHI	GGATCC	1 cut(s)	1300
BanI	GGYRCC	2 cut(s)	1088, 1771
BanII	GRGCYC	1 cut(s)	764
Bbv12I	GWGCWC	3 cut(s)	764, 1336, 1761
BbvI	GCAGC	3 cut(s)	259, 961, 1240
BccI	CCATC	7 cut(s)	194, 283, 1022, 1340, 1593, 1658, 1928
BceAI	ACGGC	1 cut(s)	203
BciT130I	CCWGG	3 cut(s)	894, 1484, 1650
BcoDI	GTCTC	3 cut(s)	45, 1277, 1526
BfaI	CTAG	1 cut(s)	1442
BfmI	CTRYAG	3 cut(s)	276, 465, 501
BfoI	RGCGCY	1 cut(s)	21
BfrI	CTTAAG	1 cut(s)	794
BglII	AGATCT	1 cut(s)	1856
BisI	GCNGC	5 cut(s)	205, 248, 950, 1035, 1254
BlsI	GCNGC	5 cut(s)	206, 249, 951, 1036, 1255
Bme1390I	CCNGG	3 cut(s)	894, 1484, 1650
Bme18I	GGWCC	1 cut(s)	1580
BmeT110I	CYCGRG	1 cut(s)	1497
BmgBI	CACGTC	1 cut(s)	125
BmgT120I	GGNCC	3 cut(s)	964, 1244, 1580
BmiI	GGNNCC	5 cut(s)	1090, 1302, 1468, 1582, 1773
BmrFI	CCNGG	3 cut(s)	894, 1484, 1650
BmrI	ACTGGG	1 cut(s)	983
BmsI	GCATC	3 cut(s)	791, 1062, 1202
BmuI	ACTGGG	1 cut(s)	983
BplI	GAGNNNNNCTC	2 cut(s)	695, 727
BpmI	CTGGAG	1 cut(s)	1350
Bsa29I	ATCGAT	1 cut(s)	344
BsaHI	GRCGYC	2 cut(s)	38, 60
BsaI	GGTCTC	2 cut(s)	1277, 1526
BsaJI	CCNNGG	8 cut(s)	63, 332, 604, 702, 813, 892, 1236, 1482
BsaXI	ACNNNNNCTCC	2 cut(s)	1504, 1534
Bsc4I	CCNNNNNNNGG	5 cut(s)	162, 883, 937, 1483, 1782
Bse118I	RCCGGY	1 cut(s)	155
Bse1I	ACTGG	6 cut(s)	111, 151, 241, 989, 1546, 1623
Bse3DI	GCAATG	2 cut(s)	1434, 1615
BseBI	CCWGG	3 cut(s)	894, 1484, 1650
BseCI	ATCGAT	1 cut(s)	344
BseDI	CCNNGG	8 cut(s)	63, 332, 604, 702, 813, 892, 1236, 1482
BseGI	GGATG	8 cut(s)	294, 430, 475, 526, 698, 1351, 1669, 1885
BseLI	CCNNNNNNNGG	5 cut(s)	162, 883, 937, 1483, 1782
BseMI	GCAATG	2 cut(s)	1434, 1615
BseMII	CTCAG	7 cut(s)	5, 14, 678, 777, 1146, 1284, 1878
BseNI	ACTGG	6 cut(s)	111, 151, 241, 989, 1546, 1623
BseRI	GAGGAG	3 cut(s)	39, 42, 1124
BseSI	GKGCMC	1 cut(s)	1774
BseXI	GCAGC	3 cut(s)	259, 961, 1240
BsgI	GTGCAG	1 cut(s)	339
BshFI	GGCC	5 cut(s)	204, 317, 965, 1245, 1476
BshNI	GGYRCC	2 cut(s)	1088, 1771
BshVI	ATCGAT	1 cut(s)	344
BsiHKAI	GWGCWC	3 cut(s)	764, 1336, 1761
BsiHKCI	CYCGRG	1 cut(s)	1497
BsiSI	CCGG	2 cut(s)	138, 156
BslFI	GGGAC	2 cut(s)	758, 1598
BslI	CCNNNNNNNGG	5 cut(s)	162, 883, 937, 1483, 1782
BsmAI	GTCTC	3 cut(s)	45, 1277, 1526
BsmBI	CGTCTC	1 cut(s)	45
BsmFI	GGGAC	2 cut(s)	758, 1598
BsmI	GAATGC	2 cut(s)	1453, 1946
BsnI	GGCC	5 cut(s)	204, 317, 965, 1245, 1476
Bso31I	GGTCTC	2 cut(s)	1277, 1526
BsoBI	CYCGRG	1 cut(s)	1497
Bsp1286I	GDGCHC	4 cut(s)	764, 1336, 1761, 1774
Bsp143I	GATC	5 cut(s)	268, 328, 1300, 1556, 1856
BspACI	CCGC	7 cut(s)	5, 108, 205, 570, 1034, 1309, 1416
BspANI	GGCC	5 cut(s)	204, 317, 965, 1245, 1476
BspCNI	CTCAG	7 cut(s)	6, 15, 677, 776, 1147, 1285, 1879
BspDI	ATCGAT	1 cut(s)	344
BspHI	TCATGA	1 cut(s)	1396
BspLI	GGNNCC	5 cut(s)	1090, 1302, 1468, 1582, 1773
BspPI	GGATC	5 cut(s)	263, 323, 1295, 1308, 1564
BspT107I	GGYRCC	2 cut(s)	1088, 1771
BspTI	CTTAAG	1 cut(s)	794
BspTNI	GGTCTC	2 cut(s)	1277, 1526
BsrDI	GCAATG	2 cut(s)	1434, 1615
BsrFI	RCCGGY	1 cut(s)	155
BsrI	ACTGG	6 cut(s)	111, 151, 241, 989, 1546, 1623
BssAI	RCCGGY	1 cut(s)	155
BssECI	CCNNGG	8 cut(s)	63, 332, 604, 702, 813, 892, 1236, 1482
BssMI	GATC	5 cut(s)	268, 328, 1300, 1556, 1856
BssNI	GRCGYC	2 cut(s)	38, 60
BssT1I	CCWWGG	3 cut(s)	63, 332, 813
Bst2UI	CCWGG	3 cut(s)	894, 1484, 1650
Bst4CI	ACNGT	5 cut(s)	626, 1088, 1338, 1372, 1636
Bst6I	CTCTTC	1 cut(s)	1812
BstACI	GRCGYC	2 cut(s)	38, 60
BstAFI	CTTAAG	1 cut(s)	794
BstAPI	GCANNNNNTGC	1 cut(s)	253
BstC8I	GCNNGC	6 cut(s)	219, 372, 776, 780, 1168, 1408
BstDEI	CTNAG	8 cut(s)	14, 23, 233, 664, 763, 1155, 1293, 1887
BstDSI	CCRYGG	1 cut(s)	604
BstENI	CCTNNNNNAGG	1 cut(s)	1780
BstF5I	GGATG	8 cut(s)	294, 430, 475, 526, 698, 1351, 1669, 1885
BstH2I	RGCGCY	1 cut(s)	21
BstHHI	GCGC	1 cut(s)	20
BstKTI	GATC	5 cut(s)	271, 331, 1303, 1559, 1859
BstMAI	GTCTC	3 cut(s)	45, 1277, 1526
BstMBI	GATC	5 cut(s)	268, 328, 1300, 1556, 1856
BstMWI	GCNNNNNNNGC	3 cut(s)	253, 1756, 1950
BstNI	CCWGG	3 cut(s)	894, 1484, 1650
BstNSI	RCATGY	1 cut(s)	1410
BstSCI	CCNGG	3 cut(s)	892, 1482, 1648
BstSFI	CTRYAG	3 cut(s)	276, 465, 501
BstSLI	GKGCMC	1 cut(s)	1774
BstV1I	GCAGC	3 cut(s)	259, 961, 1240
BstX2I	RGATCY	5 cut(s)	268, 328, 1300, 1556, 1856
BstXI	CCANNNNNNTGG	1 cut(s)	418
BstYI	RGATCY	5 cut(s)	268, 328, 1300, 1556, 1856
Bsu15I	ATCGAT	1 cut(s)	344
BsuRI	GGCC	5 cut(s)	204, 317, 965, 1245, 1476
BsuTUI	ATCGAT	1 cut(s)	344
BtgI	CCRYGG	1 cut(s)	604
BtrI	CACGTC	1 cut(s)	125
BtsCI	GGATG	8 cut(s)	294, 430, 475, 526, 698, 1351, 1669, 1885
BtsI	GCAGTG	1 cut(s)	351
BtsIMutI	CAGTG	8 cut(s)	118, 144, 248, 351, 609, 1616, 1911, 1930
Cac8I	GCNNGC	6 cut(s)	219, 372, 776, 780, 1168, 1408
CaiI	CAGNNNCTG	1 cut(s)	1342
CciI	TCATGA	1 cut(s)	1396
CfoI	GCGC	1 cut(s)	20
Cfr10I	RCCGGY	1 cut(s)	155
Cfr13I	GGNCC	3 cut(s)	964, 1244, 1580
ClaI	ATCGAT	1 cut(s)	344
CseI	GACGC	2 cut(s)	27, 68
Csp6I	GTAC	4 cut(s)	846, 1084, 1539, 1873
CviAII	CATG	9 cut(s)	638, 1052, 1397, 1407, 1478, 1535, 1547, 1767, 1896
CviQI	GTAC	4 cut(s)	846, 1084, 1539, 1873
DdeI	CTNAG	8 cut(s)	14, 23, 233, 664, 763, 1155, 1293, 1887
DpnI	GATC	5 cut(s)	270, 330, 1302, 1558, 1858
DpnII	GATC	5 cut(s)	268, 328, 1300, 1556, 1856
EaeI	YGGCCR	3 cut(s)	202, 315, 1474
Eam1104I	CTCTTC	1 cut(s)	1812
EarI	CTCTTC	1 cut(s)	1812
EciI	GGCGGA	2 cut(s)	20, 97
Ecl136II	GAGCTC	1 cut(s)	762
Eco130I	CCWWGG	3 cut(s)	63, 332, 813
Eco24I	GRGCYC	1 cut(s)	764
Eco31I	GGTCTC	2 cut(s)	1277, 1526
Eco47I	GGWCC	1 cut(s)	1580
Eco47III	AGCGCT	1 cut(s)	19
Eco53kI	GAGCTC	1 cut(s)	762
Eco57I	CTGAAG	4 cut(s)	327, 879, 1001, 1922
Eco88I	CYCGRG	1 cut(s)	1497
EcoICRI	GAGCTC	1 cut(s)	762
EcoNI	CCTNNNNNAGG	1 cut(s)	1780
EcoO109I	RGGNCCY	1 cut(s)	1580
EcoRI	GAATTC	1 cut(s)	128
EcoRII	CCWGG	3 cut(s)	892, 1482, 1648
EcoT14I	CCWWGG	3 cut(s)	63, 332, 813
EcoT22I	ATGCAT	1 cut(s)	913
EcoT38I	GRGCYC	1 cut(s)	764
ErhI	CCWWGG	3 cut(s)	63, 332, 813
Esp3I	CGTCTC	1 cut(s)	45
FaeI	CATG	9 cut(s)	641, 1055, 1400, 1410, 1481, 1538, 1550, 1770, 1899
FalI	AAGNNNNNCTT	4 cut(s)	929, 961, 1730, 1762
FaqI	GGGAC	2 cut(s)	758, 1598
FatI	CATG	9 cut(s)	637, 1051, 1396, 1406, 1477, 1534, 1546, 1766, 1895
FauNDI	CATATG	1 cut(s)	881
Fnu4HI	GCNGC	5 cut(s)	205, 248, 950, 1035, 1254
FokI	GGATG	8 cut(s)	301, 437, 462, 533, 685, 1358, 1676, 1892
FriOI	GRGCYC	1 cut(s)	764
Fsp4HI	GCNGC	5 cut(s)	205, 248, 950, 1035, 1254
FspBI	CTAG	1 cut(s)	1442
GlaI	GCGC	1 cut(s)	19
GluI	GCNGC	5 cut(s)	205, 248, 950, 1035, 1254
GsuI	CTGGAG	1 cut(s)	1350
HaeII	RGCGCY	1 cut(s)	21
HaeIII	GGCC	5 cut(s)	204, 317, 965, 1245, 1476
HapII	CCGG	2 cut(s)	138, 156
HgaI	GACGC	2 cut(s)	27, 68
HhaI	GCGC	1 cut(s)	20
Hin1I	GRCGYC	2 cut(s)	38, 60
Hin1II	CATG	9 cut(s)	641, 1055, 1400, 1410, 1481, 1538, 1550, 1770, 1899
Hin6I	GCGC	1 cut(s)	18
HinP1I	GCGC	1 cut(s)	18
HindIII	AAGCTT	1 cut(s)	1219
HinfI	GANTC	4 cut(s)	1325, 1625, 1868, 1883
HpaII	CCGG	2 cut(s)	138, 156
HphI	GGTGA	4 cut(s)	948, 1171, 1263, 1745
Hpy166II	GTNNAC	3 cut(s)	682, 709, 1573
Hpy8I	GTNNAC	3 cut(s)	682, 709, 1573
HpyCH4III	ACNGT	5 cut(s)	626, 1088, 1338, 1372, 1636
HpyCH4IV	ACGT	2 cut(s)	124, 1692
HpyF10VI	GCNNNNNNNGC	3 cut(s)	253, 1756, 1950
HpyF3I	CTNAG	8 cut(s)	14, 23, 233, 664, 763, 1155, 1293, 1887
HpySE526I	ACGT	2 cut(s)	124, 1692
Hsp92I	GRCGYC	2 cut(s)	38, 60
Hsp92II	CATG	9 cut(s)	641, 1055, 1400, 1410, 1481, 1538, 1550, 1770, 1899
HspAI	GCGC	1 cut(s)	18
Kzo9I	GATC	5 cut(s)	268, 328, 1300, 1556, 1856
LmnI	GCTCC	2 cut(s)	367, 1331
Lsp1109I	GCAGC	3 cut(s)	259, 961, 1240
LweI	GCATC	3 cut(s)	791, 1062, 1202
MaeI	CTAG	1 cut(s)	1442
MaeII	ACGT	2 cut(s)	124, 1692
MaeIII	GTNAC	8 cut(s)	113, 236, 652, 954, 1159, 1430, 1636, 1733
MalI	GATC	5 cut(s)	270, 330, 1302, 1558, 1858
MboI	GATC	5 cut(s)	268, 328, 1300, 1556, 1856
MboII	GAAGA	9 cut(s)	92, 187, 278, 664, 872, 994, 1634, 1829, 1915
MflI	RGATCY	5 cut(s)	268, 328, 1300, 1556, 1856
MhlI	GDGCHC	4 cut(s)	764, 1336, 1761, 1774
MlsI	TGGCCA	1 cut(s)	1476
MluNI	TGGCCA	1 cut(s)	1476
MlyI	GAGTC	2 cut(s)	1319, 1862
MmeI	TCCRAC	6 cut(s)	978, 1041, 1158, 1278, 1803, 1913
Mox20I	TGGCCA	1 cut(s)	1476
Mph1103I	ATGCAT	1 cut(s)	913
MscI	TGGCCA	1 cut(s)	1476
MseI	TTAA	7 cut(s)	32, 459, 795, 1007, 1217, 1790, 1808
MslI	CAYNNNNRTG	2 cut(s)	361, 1925
Msp20I	TGGCCA	1 cut(s)	1476
MspCI	CTTAAG	1 cut(s)	794
MspI	CCGG	2 cut(s)	138, 156
MspR9I	CCNGG	3 cut(s)	894, 1484, 1650
Mva1269I	GAATGC	2 cut(s)	1453, 1946
MvaI	CCWGG	3 cut(s)	894, 1484, 1650
MwoI	GCNNNNNNNGC	3 cut(s)	253, 1756, 1950
NdeI	CATATG	1 cut(s)	881
NdeII	GATC	5 cut(s)	268, 328, 1300, 1556, 1856
NlaIII	CATG	9 cut(s)	641, 1055, 1400, 1410, 1481, 1538, 1550, 1770, 1899
NlaIV	GGNNCC	5 cut(s)	1090, 1302, 1468, 1582, 1773
NmuCI	GTSAC	6 cut(s)	113, 236, 954, 1159, 1430, 1733
NsiI	ATGCAT	1 cut(s)	913
NspI	RCATGY	1 cut(s)	1410
PaeI	GCATGC	1 cut(s)	1410
PaeR7I	CTCGAG	1 cut(s)	1497
PagI	TCATGA	1 cut(s)	1396
PctI	GAATGC	2 cut(s)	1453, 1946
PfeI	GAWTC	2 cut(s)	1625, 1883
PflMI	CCANNNNNTGG	1 cut(s)	1483
PkrI	GCNGC	5 cut(s)	206, 249, 951, 1036, 1255
PleI	GAGTC	2 cut(s)	1319, 1862
PpsI	GAGTC	2 cut(s)	1319, 1862
PpuMI	RGGWCCY	1 cut(s)	1580
PshBI	ATTAAT	1 cut(s)	459
PsiI	TTATAA	2 cut(s)	170, 1700
Psp124BI	GAGCTC	1 cut(s)	764
Psp5II	RGGWCCY	1 cut(s)	1580
Psp6I	CCWGG	3 cut(s)	892, 1482, 1648
PspGI	CCWGG	3 cut(s)	892, 1482, 1648
PspN4I	GGNNCC	5 cut(s)	1090, 1302, 1468, 1582, 1773
PspPI	GGNCC	3 cut(s)	964, 1244, 1580
PspPPI	RGGWCCY	1 cut(s)	1580
PspXI	VCTCGAGB	1 cut(s)	1497
PstNI	CAGNNNCTG	1 cut(s)	1342
PsuI	RGATCY	5 cut(s)	268, 328, 1300, 1556, 1856
RsaI	GTAC	4 cut(s)	847, 1085, 1540, 1874
RsaNI	GTAC	4 cut(s)	846, 1084, 1539, 1873
RseI	CAYNNNNRTG	2 cut(s)	361, 1925
SacI	GAGCTC	1 cut(s)	764
SaqAI	TTAA	7 cut(s)	32, 459, 795, 1007, 1217, 1790, 1808
SatI	GCNGC	5 cut(s)	205, 248, 950, 1035, 1254
Sau3AI	GATC	5 cut(s)	268, 328, 1300, 1556, 1856
Sau96I	GGNCC	3 cut(s)	964, 1244, 1580
SchI	GAGTC	2 cut(s)	1319, 1862
ScrFI	CCNGG	3 cut(s)	894, 1484, 1650
SduI	GDGCHC	4 cut(s)	764, 1336, 1761, 1774
SfaNI	GCATC	3 cut(s)	791, 1062, 1202
SfcI	CTRYAG	3 cut(s)	276, 465, 501
Sfr274I	CTCGAG	1 cut(s)	1497
SinI	GGWCC	1 cut(s)	1580
SlaI	CTCGAG	1 cut(s)	1497
SmiMI	CAYNNNNRTG	2 cut(s)	361, 1925
SmlI	CTYRAG	2 cut(s)	794, 1497
SmoI	CTYRAG	2 cut(s)	794, 1497
SphI	GCATGC	1 cut(s)	1410
SsiI	CCGC	7 cut(s)	5, 108, 205, 570, 1034, 1309, 1416
SspMI	CTAG	1 cut(s)	1442
SstI	GAGCTC	1 cut(s)	764
StyD4I	CCNGG	3 cut(s)	892, 1482, 1648
StyI	CCWWGG	3 cut(s)	63, 332, 813
TaaI	ACNGT	5 cut(s)	626, 1088, 1338, 1372, 1636
TaiI	ACGT	2 cut(s)	127, 1695
TaqI	TCGA	6 cut(s)	344, 1024, 1136, 1498, 1524, 1531
TauI	GCSGC	2 cut(s)	207, 1037
TfiI	GAWTC	2 cut(s)	1625, 1883
Tru1I	TTAA	7 cut(s)	32, 459, 795, 1007, 1217, 1790, 1808
Tru9I	TTAA	7 cut(s)	32, 459, 795, 1007, 1217, 1790, 1808
TscAI	CASTG	8 cut(s)	118, 151, 248, 358, 616, 1623, 1918, 1930
TseFI	GTSAC	6 cut(s)	113, 236, 954, 1159, 1430, 1733
TseI	GCWGC	3 cut(s)	247, 949, 1253
Tsp45I	GTSAC	6 cut(s)	113, 236, 954, 1159, 1430, 1733
TspDTI	ATGAA	8 cut(s)	195, 441, 464, 626, 687, 1364, 1659, 1896
TspGWI	ACGGA	2 cut(s)	593, 744
TspRI	CASTG	8 cut(s)	118, 151, 248, 358, 616, 1623, 1918, 1930
Van91I	CCANNNNNTGG	1 cut(s)	1483
Vha464I	CTTAAG	1 cut(s)	794
VpaK11BI	GGWCC	1 cut(s)	1580
VspI	ATTAAT	1 cut(s)	459
XagI	CCTNNNNNAGG	1 cut(s)	1780
XapI	RAATTY	5 cut(s)	128, 415, 1204, 1669, 1721
XceI	RCATGY	1 cut(s)	1410
XcmI	CCANNNNNNNNNTGG	1 cut(s)	418
XhoI	CTCGAG	1 cut(s)	1497
XspI	CTAG	1 cut(s)	1442
Zsp2I	ATGCAT	1 cut(s)	913

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.