Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011160

Physical Location & Seq

Genomic Coordinates Forward (+)

22970321 .. 22976117

Gene Structure 5797 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1267200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGGCGGACACTCTCGCCGCTCTGAGGTCTCTAATGGCGTGCCACTCTCCGCCTCTCGACGCCTTGGTCGTCCCTTCTGAAGATTATCACCAGAGTGAGTATGTTTCGGCACGAGACAAACGGCGTGAGTTCGTTTCCGGCTTCACCGGGAGTGCTGGTTTGGCACTTATAACGAAGAATGAAGCGCGGCTATGGACCGATGGTCGGTACTTTTTGCAGGCAACGCAGGAACTCAGTGACCAGTGGCAGCTTATGCGAATGGGAGAAGACCCGACTGTCGATGTCTGGATGGCAAATAATCTTCCTGAAGGTTCAGCCATTGGTATTGATCCTTGGTGCACGTCAATAAACACCGTGCAACTATGGGAGCGTGCCTTTGCCAAGAAACGACAGAAGTTGGTTCAGACTTCCACAAATTTGGTGGACGAAGTTTGGAAAAATCGGCCCCCTGTGGAAATTAATCCCGTCATTGTACATCCTTTGGAATATGCTGGTCGTTCTGTTGTGGATAAGTTGAAGGATTTAAGACAAAAAATCGTACAAGGGAACACTCGTGGTATAATTATCTCAGCGCTTGATGAGGTTGCTTGGTTGTATAATATTCGTGGCACTGATGTTGCCTACAGTCCAGTTGTTCATGCATTTGCTATCGTGACATCCAACTCGGCTTTCTTTTATGTTGACAAAATGAAGGTTTCCGCTGAGGTAAACTCTTATTTGGAAGAAAATGGGATTGATGTCCGGGACTATAAAGCAGTGAGCTCGGATGTTGCCTTGCTTGCGTCTAATCAGCTCAAACCTTCATCTACAGTTAAAGGAACTGAAGCTGAAAAGGCTAATGGTGGAAACAAAGCAGAAGAAACAGATAATGACCTCATATGGGCTGACCCTGGTTCATGCTGTTATGCTTTGTATTCAAGACTGAACCCTGAAAAAGTTATTTTACAGCAGTCGCCTTTGGCCCTAGCAAAAGCTCTAAAGAACCCGGTCGAATTGGAAGGGTTAAAGAAAGCTCATATCCGGGATGGTGCAGCTGTTGTGCAATATCTTGTGTGGTTTGATAAGCAGGCGCAGGAGATATATGGGGCTTCTGGTTTCTTTTTGGAGGGGGAGGCAACAGATAAGAAAAACCACTTGAAAACTACAAAGCTAACCGAGGTGACTGTAAGTGATAAGCTGGAGGGCTTTCGAGCATCAAAAGAGAACTTTAGAGGTTTAAGTTTCCCTACTATTTCGTCTGTTGGCCCAAATGCGGCAATCATGCATTATTCACCGGAAGCAGAAACTTGTGCTGAGTTTGATCCAGACAGCATATATCTTTTTGACTCTGGGGCACAGTATCTTGATGGAACAACTGATATAACACGGACGGTTCATTTTGGTAAACCTTCAGCGCATGAGAAAGCATGCTATACGGCAGTCCTCAAAGGTCATATAGGTCTCGGGAATGCTCGATTTCCTAATGGAACCAATGGTCATGCCCTTGACATTCTTGCTCGAGTTCCTTTGTGGAAGAGTGGTCTAGATTATCGACATGGTACTGGTCATGGGATTGGGTCTTATCTAAATGTTCATGAAGGTCCACATTCGATTAGTTTCAGACCACATGCTCGAAATGTCCCGCTGCAAGCTTCCATGACTGTAACAGATGAACCTGGATACTACGAGGACGGCAATTTCGGTATAAGATTGGAGAACGTGCTTATAGTCAACGAAGCTGATACAAAATTCAACTTCGGTGACAAGGGTTACTTACAATTTGAGCATATAACTTGGGCACCATACCAGAGAAAGTTGATCGACTTGAGTCTTTTAGTACCTGAAGAATTGGAATGGCTCAACACCTACCATGCCAAATGCAAGGATATTCTGGCTCCATACTTGGATGAATCTGAGAAGGCGTGGCTGAAAAGAGCCACTGAACCCATAAGCGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006508 GO:0006807 GO:0006810 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008238 GO:0008270 GO:0009914 GO:0009926 GO:0010013 GO:0010817 GO:0016020 GO:0016787 GO:0019538 GO:0030145 GO:0031406 GO:0033218 GO:0033293 GO:0036094 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:0070011 GO:0071704 GO:0071944 GO:0097159 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Creatinase_N Creatinase_N_2 Peptidase_M24 Peptidase_M24_C

Protein Analysis

646

Amino Acids

71.74

Weight (kDa)

5.64

Isoelectric Point (pI)

35.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Creatinase_N	PF01321	6 - 138	7.7e-12	Creatinase/Prolidase N-terminal domain
Creatinase_N_2	PF16189	147 - 331	1.6e-46	Creatinase/Prolidase N-terminal domain
Peptidase_M24	PF00557	375 - 572	8.6e-47	Metallopeptidase family M24
Peptidase_M24_C	PF16188	583 - 643	5.8e-25	C-terminal region of peptidase_M24

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011160)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G36760 AT4G36760 AT4G36760
fragaria_vesca	FvH4_1g13980
malus_domestica	MD02G1152800.v1.1 MD15G1267200.v1.1
prunus_persica	Prupe.7G149300_v2.0.a1
pyrus_communis	pycom02g12060 pycom15g23280
rosa_chinensis	RchiOBHm_Chr2g0102281
rosa_laevigata	RLG00000017142 RLG00000017146
rosa_multiflora	Rmu_sc0005055.1_g000022 Rmu_sc0030874.1_g000002
rosa_roxburghii	Rroxscaffold_2G00140610
rosa_rugosa	Rorug02G0106100
rosa_samantha	Rh2AG154100 Rh2CG159700 Rh2DG159700
rosa_wichuraiana	Rw2G012050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	170
AasI	GACNNNNNNGTC	2 cut(s)	65, 275
AccB1I	GGYRCC	1 cut(s)	1777
AccBSI	CCGCTC	1 cut(s)	20
AccII	CGCG	1 cut(s)	187
AciI	CCGC	7 cut(s)	5, 18, 50, 187, 699, 1253, 1622
AclWI	GGATC	2 cut(s)	323, 1295
AcsI	RAATTY	2 cut(s)	415, 1727
AcuI	CTGAAG	5 cut(s)	99, 327, 843, 1374, 1842
AcyI	GRCGYC	1 cut(s)	60
AfaI	GTAC	5 cut(s)	209, 474, 540, 1540, 1818
AfeI	AGCGCT	1 cut(s)	573
AfiI	CCNNNNNNNGG	4 cut(s)	24, 204, 880, 1252
AgsI	TTSAA	4 cut(s)	517, 918, 1138, 1732
AhdI	GACNNNNNGTC	1 cut(s)	201
AjiI	CACGTC	1 cut(s)	342
AjnI	CCWGG	2 cut(s)	889, 1654
AjuI	GAANNNNNNNTTGG	2 cut(s)	653, 685
AleI	CACNNNNGTG	1 cut(s)	93
Alw21I	GWGCWC	2 cut(s)	341, 764
Alw26I	GTCTC	3 cut(s)	33, 108, 1445
Alw44I	GTGCAC	1 cut(s)	337
AlwI	GGATC	2 cut(s)	323, 1295
Ama87I	CYCGRG	2 cut(s)	1442, 1497
Aor51HI	AGCGCT	1 cut(s)	573
AoxI	GGCC	3 cut(s)	443, 960, 1243
ApaLI	GTGCAC	1 cut(s)	337
ApeKI	GCWGC	3 cut(s)	247, 1031, 1624
ApoI	RAATTY	2 cut(s)	415, 1727
ArsI	GACNNNNNNTTYG	2 cut(s)	1661, 1693
AseI	ATTAAT	1 cut(s)	459
AspLEI	GCGC	4 cut(s)	187, 574, 1072, 1396
AspS9I	GGNCC	5 cut(s)	195, 444, 961, 1244, 1580
AsuC2I	CCSGG	4 cut(s)	148, 743, 986, 1022
AsuHPI	GGTGA	5 cut(s)	80, 136, 1171, 1263, 1751
AvaI	CYCGRG	2 cut(s)	1442, 1497
AvaII	GGWCC	2 cut(s)	195, 1580
BaeGI	GKGCMC	3 cut(s)	341, 1336, 1780
BanI	GGYRCC	1 cut(s)	1777
BanII	GRGCYC	1 cut(s)	764
BauI	CACGAG	2 cut(s)	111, 552
BbsI	GAAGAC	1 cut(s)	273
Bbv12I	GWGCWC	2 cut(s)	341, 764
BbvCI	CCTCAGC	1 cut(s)	702
BbvI	GCAGC	3 cut(s)	259, 1043, 1611
BccI	CCATC	4 cut(s)	194, 283, 1019, 1340
BceAI	ACGGC	3 cut(s)	137, 1431, 1687
BcgI	CGANNNNNNTGC	2 cut(s)	744, 778
BciT130I	CCWGG	2 cut(s)	891, 1656
BciVI	GTATCC	1 cut(s)	1652
BcnI	CCSGG	4 cut(s)	148, 743, 986, 1022
BcoDI	GTCTC	3 cut(s)	33, 108, 1445
BfaI	CTAG	2 cut(s)	965, 1523
BfmI	CTRYAG	2 cut(s)	622, 807
BfoI	RGCGCY	1 cut(s)	575
BfuI	GTATCC	1 cut(s)	1652
BisI	GCNGC	6 cut(s)	18, 188, 248, 1032, 1254, 1625
BlsI	GCNGC	6 cut(s)	19, 189, 249, 1033, 1255, 1626
Bme1390I	CCNGG	6 cut(s)	148, 743, 891, 986, 1022, 1656
Bme18I	GGWCC	2 cut(s)	195, 1580
BmeRI	GACNNNNNGTC	1 cut(s)	201
BmeT110I	CYCGRG	2 cut(s)	1442, 1497
BmgBI	CACGTC	1 cut(s)	342
BmgT120I	GGNCC	5 cut(s)	195, 444, 961, 1244, 1580
BmiI	GGNNCC	4 cut(s)	446, 1468, 1779, 1875
BmrFI	CCNGG	6 cut(s)	148, 743, 891, 986, 1022, 1656
BmsI	GCATC	1 cut(s)	1202
BoxI	GACNNNNGTC	1 cut(s)	1806
BpiI	GAAGAC	1 cut(s)	273
BplI	GAGNNNNNCTC	2 cut(s)	695, 727
BpmI	CTGGAG	1 cut(s)	1199
Bpu10I	CCTNAGC	1 cut(s)	702
BpuEI	CTTGAG	1 cut(s)	1825
BpuMI	CCSGG	4 cut(s)	148, 743, 986, 1022
BsaHI	GRCGYC	1 cut(s)	60
BsaI	GGTCTC	2 cut(s)	33, 1445
BsaJI	CCNNGG	4 cut(s)	63, 332, 889, 1155
BsaWI	WCCGGW	1 cut(s)	1273
BsaXI	ACNNNNNCTCC	2 cut(s)	142, 172
Bsc4I	CCNNNNNNNGG	4 cut(s)	24, 204, 880, 1252
Bse1I	ACTGG	3 cut(s)	241, 629, 1546
BseBI	CCWGG	2 cut(s)	891, 1656
BseDI	CCNNGG	4 cut(s)	63, 332, 889, 1155
BseGI	GGATG	6 cut(s)	294, 475, 656, 772, 1030, 1891
BseLI	CCNNNNNNNGG	4 cut(s)	24, 204, 880, 1252
BseMII	CTCAG	6 cut(s)	14, 247, 582, 693, 1284, 1884
BseNI	ACTGG	3 cut(s)	241, 629, 1546
BseSI	GKGCMC	3 cut(s)	341, 1336, 1780
BseXI	GCAGC	3 cut(s)	259, 1043, 1611
BsgI	GTGCAG	1 cut(s)	1050
Bsh1236I	CGCG	1 cut(s)	187
Bsh1285I	CGRYCG	1 cut(s)	990
BshFI	GGCC	3 cut(s)	445, 962, 1245
BshNI	GGYRCC	1 cut(s)	1777
BsiEI	CGRYCG	1 cut(s)	990
BsiHKAI	GWGCWC	2 cut(s)	341, 764
BsiHKCI	CYCGRG	2 cut(s)	1442, 1497
BsiSI	CCGG	6 cut(s)	138, 147, 742, 986, 1021, 1274
BslFI	GGGAC	3 cut(s)	56, 758, 1604
BslI	CCNNNNNNNGG	4 cut(s)	24, 204, 880, 1252
BsmAI	GTCTC	3 cut(s)	33, 108, 1445
BsmFI	GGGAC	3 cut(s)	56, 758, 1604
BsmI	GAATGC	1 cut(s)	1453
BsnI	GGCC	3 cut(s)	445, 962, 1245
Bso31I	GGTCTC	2 cut(s)	33, 1445
BsoBI	CYCGRG	2 cut(s)	1442, 1497
Bsp1286I	GDGCHC	4 cut(s)	341, 764, 1336, 1780
Bsp1407I	TGTACA	1 cut(s)	472
Bsp143I	GATC	3 cut(s)	328, 1300, 1797
BspACI	CCGC	7 cut(s)	5, 18, 50, 187, 699, 1253, 1622
BspANI	GGCC	3 cut(s)	445, 962, 1245
BspCNI	CTCAG	6 cut(s)	15, 246, 581, 694, 1285, 1885
BspFNI	CGCG	1 cut(s)	187
BspHI	TCATGA	1 cut(s)	1573
BspLI	GGNNCC	4 cut(s)	446, 1468, 1779, 1875
BspPI	GGATC	2 cut(s)	323, 1295
BspT107I	GGYRCC	1 cut(s)	1777
BspTNI	GGTCTC	2 cut(s)	33, 1445
BsrBI	CCGCTC	1 cut(s)	20
BsrGI	TGTACA	1 cut(s)	472
BsrI	ACTGG	3 cut(s)	241, 629, 1546
BssECI	CCNNGG	4 cut(s)	63, 332, 889, 1155
BssMI	GATC	3 cut(s)	328, 1300, 1797
BssNI	GRCGYC	1 cut(s)	60
BssSI	CACGAG	2 cut(s)	111, 552
BssT1I	CCWWGG	2 cut(s)	63, 332
Bst2BI	CACGAG	2 cut(s)	111, 552
Bst2UI	CCWGG	2 cut(s)	891, 1656
Bst4CI	ACNGT	8 cut(s)	277, 355, 626, 811, 1165, 1338, 1372, 1642
Bst6I	CTCTTC	1 cut(s)	1508
BstACI	GRCGYC	1 cut(s)	60
BstAPI	GCANNNNNTGC	1 cut(s)	253
BstAUI	TGTACA	1 cut(s)	472
BstC8I	GCNNGC	7 cut(s)	40, 219, 372, 780, 1068, 1408, 1629
BstDEI	CTNAG	6 cut(s)	23, 233, 568, 702, 1293, 1893
BstF5I	GGATG	6 cut(s)	294, 475, 656, 772, 1030, 1891
BstFNI	CGCG	1 cut(s)	187
BstH2I	RGCGCY	1 cut(s)	575
BstHHI	GCGC	4 cut(s)	187, 574, 1072, 1396
BstKTI	GATC	3 cut(s)	331, 1303, 1800
BstMAI	GTCTC	3 cut(s)	33, 108, 1445
BstMBI	GATC	3 cut(s)	328, 1300, 1797
BstMCI	CGRYCG	1 cut(s)	990
BstMWI	GCNNNNNNNGC	5 cut(s)	161, 223, 253, 779, 833
BstNI	CCWGG	2 cut(s)	891, 1656
BstNSI	RCATGY	2 cut(s)	1410, 1610
BstPAI	GACNNNNGTC	1 cut(s)	1806
BstSCI	CCNGG	6 cut(s)	146, 741, 889, 984, 1020, 1654
BstSFI	CTRYAG	2 cut(s)	622, 807
BstSLI	GKGCMC	3 cut(s)	341, 1336, 1780
BstUI	CGCG	1 cut(s)	187
BstV1I	GCAGC	3 cut(s)	259, 1043, 1611
BstV2I	GAAGAC	1 cut(s)	273
BstXI	CCANNNNNNTGG	1 cut(s)	418
BsuI	GTATCC	1 cut(s)	1652
BsuRI	GGCC	3 cut(s)	445, 962, 1245
BtrI	CACGTC	1 cut(s)	342
BtsCI	GGATG	6 cut(s)	294, 475, 656, 772, 1030, 1891
BtsI	GCAGTG	1 cut(s)	762
BtsIMutI	CAGTG	5 cut(s)	241, 248, 609, 762, 1917
Cac8I	GCNNGC	7 cut(s)	40, 219, 372, 780, 1068, 1408, 1629
CciI	TCATGA	1 cut(s)	1573
CfoI	GCGC	4 cut(s)	187, 574, 1072, 1396
Cfr13I	GGNCC	5 cut(s)	195, 444, 961, 1244, 1580
CseI	GACGC	2 cut(s)	68, 771
Csp6I	GTAC	5 cut(s)	208, 473, 539, 1539, 1817
CspCI	CAANNNNNGTGG	2 cut(s)	402, 437
CviQI	GTAC	5 cut(s)	208, 473, 539, 1539, 1817
DdeI	CTNAG	6 cut(s)	23, 233, 568, 702, 1293, 1893
DpnI	GATC	3 cut(s)	330, 1302, 1799
DpnII	GATC	3 cut(s)	328, 1300, 1797
DrdI	GACNNNNNNGTC	2 cut(s)	65, 275
DriI	GACNNNNNGTC	1 cut(s)	201
DseDI	GACNNNNNNGTC	2 cut(s)	65, 275
Eam1104I	CTCTTC	1 cut(s)	1508
Eam1105I	GACNNNNNGTC	1 cut(s)	201
EarI	CTCTTC	1 cut(s)	1508
EciI	GGCGGA	2 cut(s)	20, 39
Ecl136II	GAGCTC	1 cut(s)	762
Eco130I	CCWWGG	2 cut(s)	63, 332
Eco24I	GRGCYC	1 cut(s)	764
Eco31I	GGTCTC	2 cut(s)	33, 1445
Eco47I	GGWCC	2 cut(s)	195, 1580
Eco47III	AGCGCT	1 cut(s)	573
Eco53kI	GAGCTC	1 cut(s)	762
Eco57I	CTGAAG	5 cut(s)	99, 327, 843, 1374, 1842
Eco88I	CYCGRG	2 cut(s)	1442, 1497
EcoICRI	GAGCTC	1 cut(s)	762
EcoRII	CCWGG	2 cut(s)	889, 1654
EcoT14I	CCWWGG	2 cut(s)	63, 332
EcoT22I	ATGCAT	2 cut(s)	643, 1266
EcoT38I	GRGCYC	1 cut(s)	764
ErhI	CCWWGG	2 cut(s)	63, 332
FalI	AAGNNNNNCTT	2 cut(s)	1736, 1768
FaqI	GGGAC	3 cut(s)	56, 758, 1604
FauI	CCCGC	1 cut(s)	1629
FauNDI	CATATG	1 cut(s)	878
Fnu4HI	GCNGC	6 cut(s)	18, 188, 248, 1032, 1254, 1625
FokI	GGATG	6 cut(s)	301, 462, 643, 779, 1037, 1898
FriOI	GRGCYC	1 cut(s)	764
Fsp4HI	GCNGC	6 cut(s)	18, 188, 248, 1032, 1254, 1625
FspBI	CTAG	2 cut(s)	965, 1523
GlaI	GCGC	4 cut(s)	186, 573, 1071, 1395
GluI	GCNGC	6 cut(s)	18, 188, 248, 1032, 1254, 1625
GsuI	CTGGAG	1 cut(s)	1199
HaeII	RGCGCY	1 cut(s)	575
HaeIII	GGCC	3 cut(s)	445, 962, 1245
HapII	CCGG	6 cut(s)	138, 147, 742, 986, 1021, 1274
HgaI	GACGC	2 cut(s)	68, 771
HhaI	GCGC	4 cut(s)	187, 574, 1072, 1396
Hin1I	GRCGYC	1 cut(s)	60
Hin6I	GCGC	4 cut(s)	185, 572, 1070, 1394
HinP1I	GCGC	4 cut(s)	185, 572, 1070, 1394
HincII	GTYRAC	2 cut(s)	682, 1711
HindII	GTYRAC	2 cut(s)	682, 1711
HindIII	AAGCTT	1 cut(s)	1629
HinfI	GANTC	3 cut(s)	1325, 1807, 1889
HpaII	CCGG	6 cut(s)	138, 147, 742, 986, 1021, 1274
HphI	GGTGA	5 cut(s)	80, 136, 1171, 1263, 1751
Hpy166II	GTNNAC	7 cut(s)	339, 424, 682, 709, 1385, 1583, 1711
Hpy188I	TCNGA	6 cut(s)	24, 79, 405, 766, 1601, 1894
Hpy8I	GTNNAC	7 cut(s)	339, 424, 682, 709, 1385, 1583, 1711
Hpy99I	CGWCG	1 cut(s)	62
HpyAV	CCTTC	9 cut(s)	84, 302, 511, 685, 810, 992, 1398, 1571, 1891
HpyCH4III	ACNGT	8 cut(s)	277, 355, 626, 811, 1165, 1338, 1372, 1642
HpyCH4IV	ACGT	2 cut(s)	341, 1698
HpyCH4V	TGCA	9 cut(s)	217, 339, 358, 641, 1031, 1042, 1264, 1627, 1860
HpyF10VI	GCNNNNNNNGC	5 cut(s)	161, 223, 253, 779, 833
HpyF3I	CTNAG	6 cut(s)	23, 233, 568, 702, 1293, 1893
HpySE526I	ACGT	2 cut(s)	341, 1698
Hsp92I	GRCGYC	1 cut(s)	60
HspAI	GCGC	4 cut(s)	185, 572, 1070, 1394
Kzo9I	GATC	3 cut(s)	328, 1300, 1797
LmnI	GCTCC	2 cut(s)	367, 1879
Lsp1109I	GCAGC	3 cut(s)	259, 1043, 1611
LweI	GCATC	1 cut(s)	1202
MaeI	CTAG	2 cut(s)	965, 1523
MaeII	ACGT	2 cut(s)	341, 1698
MaeIII	GTNAC	6 cut(s)	236, 652, 1159, 1642, 1739, 1748
MalI	GATC	3 cut(s)	330, 1302, 1799
MbiI	CCGCTC	1 cut(s)	20
MboI	GATC	3 cut(s)	328, 1300, 1797
MboII	GAAGA	8 cut(s)	92, 187, 278, 293, 734, 869, 1525, 1835
MhlI	GDGCHC	4 cut(s)	341, 764, 1336, 1780
MluCI	AATT	8 cut(s)	415, 456, 561, 992, 1675, 1727, 1757, 1826
MlyI	GAGTC	2 cut(s)	1319, 1816
MmeI	TCCRAC	1 cut(s)	684
Mph1103I	ATGCAT	2 cut(s)	643, 1266
MseI	TTAA	5 cut(s)	459, 524, 813, 1004, 1217
MslI	CAYNNNNRTG	2 cut(s)	93, 1931
MspA1I	CMGCKG	3 cut(s)	701, 1034, 1624
MspI	CCGG	6 cut(s)	138, 147, 742, 986, 1021, 1274
MspR9I	CCNGG	6 cut(s)	148, 743, 891, 986, 1022, 1656
Mva1269I	GAATGC	1 cut(s)	1453
MvaI	CCWGG	2 cut(s)	891, 1656
MvnI	CGCG	1 cut(s)	187
MwoI	GCNNNNNNNGC	5 cut(s)	161, 223, 253, 779, 833
NciI	CCSGG	4 cut(s)	148, 743, 986, 1022
NdeI	CATATG	1 cut(s)	878
NdeII	GATC	3 cut(s)	328, 1300, 1797
NlaIV	GGNNCC	4 cut(s)	446, 1468, 1779, 1875
NmeAIII	GCCGAG	1 cut(s)	644
NmuCI	GTSAC	4 cut(s)	236, 652, 1159, 1739
NsiI	ATGCAT	2 cut(s)	643, 1266
NspI	RCATGY	2 cut(s)	1410, 1610
OliI	CACNNNNGTG	1 cut(s)	93
PaeI	GCATGC	1 cut(s)	1410
PaeR7I	CTCGAG	1 cut(s)	1497
PagI	TCATGA	1 cut(s)	1573
PcsI	WCGNNNNNNNCGW	1 cut(s)	66
PctI	GAATGC	1 cut(s)	1453
PfeI	GAWTC	1 cut(s)	1889
PfoI	TCCNGGA	2 cut(s)	741, 1020
PkrI	GCNGC	6 cut(s)	19, 189, 249, 1033, 1255, 1626
PleI	GAGTC	2 cut(s)	1319, 1815
PpsI	GAGTC	2 cut(s)	1319, 1815
PshAI	GACNNNNGTC	1 cut(s)	1806
PshBI	ATTAAT	1 cut(s)	459
PsiI	TTATAA	1 cut(s)	170
Psp124BI	GAGCTC	1 cut(s)	764
Psp6I	CCWGG	2 cut(s)	889, 1654
PspGI	CCWGG	2 cut(s)	889, 1654
PspN4I	GGNNCC	4 cut(s)	446, 1468, 1779, 1875
PspPI	GGNCC	5 cut(s)	195, 444, 961, 1244, 1580
PspXI	VCTCGAGB	1 cut(s)	1497
PvuII	CAGCTG	1 cut(s)	1034
RsaI	GTAC	5 cut(s)	209, 474, 540, 1540, 1818
RsaNI	GTAC	5 cut(s)	208, 473, 539, 1539, 1817
RseI	CAYNNNNRTG	2 cut(s)	93, 1931
SacI	GAGCTC	1 cut(s)	764
SaqAI	TTAA	5 cut(s)	459, 524, 813, 1004, 1217
SatI	GCNGC	6 cut(s)	18, 188, 248, 1032, 1254, 1625
Sau3AI	GATC	3 cut(s)	328, 1300, 1797
Sau96I	GGNCC	5 cut(s)	195, 444, 961, 1244, 1580
SchI	GAGTC	2 cut(s)	1319, 1816
ScrFI	CCNGG	6 cut(s)	148, 743, 891, 986, 1022, 1656
SduI	GDGCHC	4 cut(s)	341, 764, 1336, 1780
SfaNI	GCATC	1 cut(s)	1202
SfcI	CTRYAG	2 cut(s)	622, 807
Sfr274I	CTCGAG	1 cut(s)	1497
SinI	GGWCC	2 cut(s)	195, 1580
SlaI	CTCGAG	1 cut(s)	1497
SmiMI	CAYNNNNRTG	2 cut(s)	93, 1931
SmlI	CTYRAG	2 cut(s)	1497, 1804
SmoI	CTYRAG	2 cut(s)	1497, 1804
SphI	GCATGC	1 cut(s)	1410
Sse9I	AATT	8 cut(s)	415, 456, 561, 992, 1675, 1727, 1757, 1826
SsiI	CCGC	7 cut(s)	5, 18, 50, 187, 699, 1253, 1622
SspI	AATATT	1 cut(s)	601
SspMI	CTAG	2 cut(s)	965, 1523
SstI	GAGCTC	1 cut(s)	764
StyD4I	CCNGG	6 cut(s)	146, 741, 889, 984, 1020, 1654
StyI	CCWWGG	2 cut(s)	63, 332
TaaI	ACNGT	8 cut(s)	277, 355, 626, 811, 1165, 1338, 1372, 1642
TaiI	ACGT	2 cut(s)	344, 1701
TaqII	GACCGA	1 cut(s)	212
TasI	AATT	8 cut(s)	415, 456, 561, 992, 1675, 1727, 1757, 1826
TatI	WGTACW	1 cut(s)	472
TauI	GCSGC	3 cut(s)	20, 190, 1256
TfiI	GAWTC	1 cut(s)	1889
Tru1I	TTAA	5 cut(s)	459, 524, 813, 1004, 1217
Tru9I	TTAA	5 cut(s)	459, 524, 813, 1004, 1217
TscAI	CASTG	5 cut(s)	241, 248, 616, 762, 1924
TseFI	GTSAC	4 cut(s)	236, 652, 1159, 1739
TseI	GCWGC	3 cut(s)	247, 1031, 1624
Tsp45I	GTSAC	4 cut(s)	236, 652, 1159, 1739
TspGWI	ACGGA	1 cut(s)	1381
TspRI	CASTG	5 cut(s)	241, 248, 616, 762, 1924
VneI	GTGCAC	1 cut(s)	337
VpaK11BI	GGWCC	2 cut(s)	195, 1580
VspI	ATTAAT	1 cut(s)	459
XapI	RAATTY	2 cut(s)	415, 1727
XbaI	TCTAGA	1 cut(s)	1522
XceI	RCATGY	2 cut(s)	1410, 1610
XcmI	CCANNNNNNNNNTGG	1 cut(s)	418
XhoI	CTCGAG	1 cut(s)	1497
XspI	CTAG	2 cut(s)	965, 1523
Zsp2I	ATGCAT	2 cut(s)	643, 1266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.