FvH4_1g17030

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013593
Physical Location & Seq
Forward (+)
9844529 .. 9848984
4456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g17030.t4

Sequence Viewer

Length: 1725 bp
ATGGCTAATGCAGAGCAAGTGGTGGCAAGTGAGACATCTCAGCCCCTGATATATCAGCTTGGTAAGGCATTACTGGAACTAAATGCTCATAATCATACTGCTGAGGACAGAGTCCAGTGGACAGAAATTGAAGAGCATTTCCTCAACCTTGAATTGACATTGAAGAAACAATCTGAAGAGCTAGAAGCCAAGGAGAAGAAATTTGAGGAGCGGGAAGCTGAAACCTGTGCTTTGCTTGTGGAGAGAGAAGCTACTGTTATTGCTAAAGAGCAAGACCTGCTGGATCGGGTGCAAGAGCTAAAAGATGCAGCGGTTGCTGCTATTGCAGAGGCACGAGACATTCACCAGCCTAAAACTATGGAACCGTTGAATGATGGGAATAACAAAGACAGCAAGGTAAGCAGCTCACTTGGTGATACAAATTCACCACAGGAAGATATACCTACTAAAAAAGGTGATAATGCTAAGGGTATGGCTAGTGACATTAAGCCACGCCAAGAACTGAGGCAATTTTGCGAGAAGATGGATGCCAAAGGGCTTCTGAATTATATTGTTGACAATCTTAAAAAACAAAATGTTGGTCGTCAGGAACTTACTATTGCACTAGAAAGTGCAAGTGAACCAGCGCAATTGGTGCTGGATTCACTGGATGGGTTTTACCCTCTTGATGAAACTAACCAACCAGAGGGTAAGAAGGATCCTGTCCTTCGGGGCAAGCGTAGATCCTGTGTCATGCTTGTGGAAGCCTTGGCTACCTTAGTTGCAAAGGCTGATCCATGTGCTGATCACTTTCTGAACCCTGAGACCAAGCAGCAAGCCAAAGCAATTGCTGATGAGTGGAGGCCTAAGTTGTTTAGTGGAGGTATTGATGCTGCCAATGGAAATTCATTGGAAGCAGAGGCATTCTTGCAGCTTCTTGCAACTTTTAGGATTGGATCTGAGTTTGATGAAGATGAACTCTGCAAGGTTGTTCTTGCAGCAGTTACTCACCGCAGGCAGACACCTGAGCTCTGCCGCTCCCTTGGTTTAACACACAAAATGCCAGGTCTTGTTGAATCAATTATCAGAATTGGGAAACAAATTGATGCTGTGCATTTTATTCATGCCTTCGAACTTACTGAAAGTTTCCCCCCTGTGCCACTCTTGAAGACATATTTGAAAGAACTGAGGAGAAATTCACAAGGCGACAGCAATGGAGATGCACAGGAGGATGTTAATGCAAAAGAGCTTTCTGGTCTGAAGGCTGTAGTTGGGTGTGTTCAAGAGTACAAGCTTGAAGCAGAGTACCCCCTTGATCCACTGCAGAAGAGGCTTGTTCAACTAGAGAGATCCAGAGCTGACAGGAAGCGAAGCGGTGATTCTGGTAAACGCCAACAACTACCAAAGAAGCAAAAAGCCACCGGAAGATGGCGTGGATACAGTGGTGGGCCTGGTCTCCTCATTGCCTCGTCCCCCACTGTTGGTAGGCATGGGCAACCTGCTTATGGCGAGAGGCCAGCATACACTGGGATGTCAGAGAGGTATGGTCTGGCCGGTCCACGCGCCTATGATTATCAAGCACCAACTCAGCCGGCTTATGCTCCACAAGCTAGTAGTGACCAAAGGCTCTATTACTACCCCCCAGAAGAGAGAGCTGCCTCAACCTCATATAACGCCGCTCCACTACCCACTTACAGCAGCTACATTGGTACTCAGTTGCAGCCTCAACACCACCCATACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

575

Amino Acids

63.83

Weight (kDa)

5.65

Isoelectric Point (pI)

53.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 163 - 451 7.2e-97 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013593)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17030 FvH4_1g17030 FvH4_1g17030 FvH4_1g17030 FvH4_1g17030
malus_domestica MD02G1180500.v1.1
prunus_persica Prupe.6G225800_v2.0.a1
pyrus_communis pycom02g14640
rosa_chinensis RchiOBHm_Chr2g0107191
rosa_laevigata RLG00000017558
rosa_multiflora Rmu_co8250165.1_g000001
rosa_roxburghii Rroxscaffold_2G00136560
rosa_rugosa Rorug02G0140600
rosa_samantha Rh2AG191400 Rh2BG203400 Rh2CG196600 Rh2DG198700
rosa_wichuraiana Rw2G015130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 285, 1486
AccBSI CCGCTC 3 cut(s) 211, 1017, 1658
AccII CGCG 1 cut(s) 1542
AciI CCGC 6 cut(s) 211, 311, 991, 1015, 1353, 1656
AclWI GGATC 8 cut(s) 291, 692, 705, 717, 767, 943, 1289, 1323
AcoI YGGCCR 1 cut(s) 1530
AcsI RAATTY 4 cut(s) 200, 421, 883, 1174
AcuI CTGAAG 2 cut(s) 195, 1259
AdeI CACNNNGTG 1 cut(s) 413
AfaI GTAC 3 cut(s) 1268, 1286, 1690
AfiI CCNNNNNNNGG 4 cut(s) 685, 1407, 1460, 1484
AflIII ACRYGT 1 cut(s) 1719
AjnI CCWGG 2 cut(s) 1042, 1429
Alw21I GWGCWC 1 cut(s) 1011
Alw26I GTCTC 4 cut(s) 26, 330, 797, 1439
AlwI GGATC 8 cut(s) 291, 692, 705, 717, 767, 943, 1289, 1323
AlwNI CAGNNNCTG 1 cut(s) 46
AoxI GGCC 4 cut(s) 842, 1427, 1493, 1530
ApoI RAATTY 4 cut(s) 200, 421, 883, 1174
ArsI GACNNNNNNTTYG 2 cut(s) 565, 597
AspLEI GCGC 2 cut(s) 628, 1544
AspS9I GGNCC 2 cut(s) 1427, 1535
AsuHPI GGTGA 6 cut(s) 335, 417, 425, 467, 980, 1367
AsuII TTCGAA 1 cut(s) 1110
AvaII GGWCC 1 cut(s) 1535
BaeI ACNNNNGTAYC 2 cut(s) 1408, 1441
BamHI GGATCC 1 cut(s) 697
BanII GRGCYC 1 cut(s) 1011
BauI CACGAG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 1154
Bbv12I GWGCWC 1 cut(s) 1011
BbvCI CCTCAGC 1 cut(s) 102
BccI CCATC 4 cut(s) 368, 517, 644, 1401
BciT130I CCWGG 2 cut(s) 1044, 1431
BciVI GTATCC 1 cut(s) 1409
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 4 cut(s) 26, 330, 797, 1439
BfaI CTAG 5 cut(s) 182, 477, 605, 1322, 1590
BfmI CTRYAG 2 cut(s) 1245, 1301
BfuAI ACCTGC 2 cut(s) 285, 1486
BfuI GTATCC 1 cut(s) 1409
Bme1390I CCNGG 2 cut(s) 1044, 1431
Bme18I GGWCC 1 cut(s) 1535
BmgT120I GGNCC 2 cut(s) 1427, 1535
BmiI GGNNCC 2 cut(s) 363, 699
BmrFI CCNGG 2 cut(s) 1044, 1431
BmrI ACTGGG 1 cut(s) 1515
BmsI GCATC 5 cut(s) 295, 517, 859, 1075, 1189
BmuI ACTGGG 1 cut(s) 1515
BpiI GAAGAC 1 cut(s) 1154
Bpu10I CCTNAGC 3 cut(s) 102, 465, 1005
Bpu14I TTCGAA 1 cut(s) 1110
BsaI GGTCTC 2 cut(s) 797, 1439
BsaJI CCNNGG 3 cut(s) 189, 747, 1021
BsaWI WCCGGW 1 cut(s) 1400
Bsc4I CCNNNNNNNGG 4 cut(s) 685, 1407, 1460, 1484
Bse118I RCCGGY 2 cut(s) 1532, 1570
Bse1I ACTGG 4 cut(s) 78, 115, 651, 1510
Bse3DI GCAATG 2 cut(s) 1198, 1440
BseBI CCWGG 2 cut(s) 1044, 1431
BseDI CCNNGG 3 cut(s) 189, 747, 1021
BseGI GGATG 4 cut(s) 532, 655, 1216, 1515
BseLI CCNNNNNNNGG 4 cut(s) 685, 1407, 1460, 1484
BseMI GCAATG 2 cut(s) 1198, 1440
BseMII CTCAG 9 cut(s) 53, 93, 494, 792, 930, 996, 1157, 1580, 1706
BseNI ACTGG 4 cut(s) 78, 115, 651, 1510
BseRI GAGGAG 3 cut(s) 221, 1183, 1427
Bsh1236I CGCG 1 cut(s) 1542
BshFI GGCC 4 cut(s) 844, 1429, 1495, 1532
BsiHKAI GWGCWC 1 cut(s) 1011
BsiSI CCGG 3 cut(s) 1401, 1533, 1571
BslFI GGGAC 1 cut(s) 1435
BslI CCNNNNNNNGG 4 cut(s) 685, 1407, 1460, 1484
BsmAI GTCTC 4 cut(s) 26, 330, 797, 1439
BsmFI GGGAC 1 cut(s) 1435
BsmI GAATGC 1 cut(s) 902
BsnI GGCC 4 cut(s) 844, 1429, 1495, 1532
Bso31I GGTCTC 2 cut(s) 797, 1439
Bsp119I TTCGAA 1 cut(s) 1110
Bsp1286I GDGCHC 1 cut(s) 1011
Bsp143I GATC 8 cut(s) 283, 697, 722, 772, 784, 935, 1294, 1328
BspACI CCGC 6 cut(s) 211, 311, 991, 1015, 1353, 1656
BspANI GGCC 4 cut(s) 844, 1429, 1495, 1532
BspCNI CTCAG 9 cut(s) 52, 94, 495, 793, 931, 997, 1158, 1579, 1705
BspFNI CGCG 1 cut(s) 1542
BspLI GGNNCC 2 cut(s) 363, 699
BspMAI CTGCAG 1 cut(s) 1305
BspMI ACCTGC 2 cut(s) 285, 1486
BspPI GGATC 8 cut(s) 291, 692, 705, 717, 767, 943, 1289, 1323
BspQI GCTCTTC 2 cut(s) 126, 171
BspT104I TTCGAA 1 cut(s) 1110
BspTNI GGTCTC 2 cut(s) 797, 1439
BsrBI CCGCTC 3 cut(s) 211, 1017, 1658
BsrDI GCAATG 2 cut(s) 1198, 1440
BsrFI RCCGGY 2 cut(s) 1532, 1570
BsrI ACTGG 4 cut(s) 78, 115, 651, 1510
BssAI RCCGGY 2 cut(s) 1532, 1570
BssECI CCNNGG 3 cut(s) 189, 747, 1021
BssMI GATC 8 cut(s) 283, 697, 722, 772, 784, 935, 1294, 1328
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 3 cut(s) 189, 747, 1021
Bst2BI CACGAG 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 1044, 1431
Bst4CI ACNGT 4 cut(s) 256, 366, 1421, 1459
Bst6I CTCTTC 4 cut(s) 126, 171, 1301, 1620
BstAPI GCANNNNNTGC 3 cut(s) 277, 314, 634
BstBI TTCGAA 1 cut(s) 1110
BstC8I GCNNGC 5 cut(s) 716, 816, 995, 1497, 1572
BstF5I GGATG 4 cut(s) 532, 655, 1216, 1515
BstFNI CGCG 1 cut(s) 1542
BstHHI GCGC 2 cut(s) 628, 1544
BstKTI GATC 8 cut(s) 286, 700, 725, 775, 787, 938, 1297, 1331
BstMAI GTCTC 4 cut(s) 26, 330, 797, 1439
BstMBI GATC 8 cut(s) 283, 697, 722, 772, 784, 935, 1294, 1328
BstMWI GCNNNNNNNGC 8 cut(s) 277, 314, 317, 323, 399, 634, 1309, 1586
BstNI CCWGG 2 cut(s) 1044, 1431
BstNSI RCATGY 1 cut(s) 1723
BstSCI CCNGG 2 cut(s) 1042, 1429
BstSFI CTRYAG 2 cut(s) 1245, 1301
BstUI CGCG 1 cut(s) 1542
BstV2I GAAGAC 1 cut(s) 1154
BstX2I RGATCY 4 cut(s) 697, 722, 935, 1328
BstYI RGATCY 4 cut(s) 697, 722, 935, 1328
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 4 cut(s) 844, 1429, 1495, 1532
BtsCI GGATG 4 cut(s) 532, 655, 1216, 1515
BtsI GCAGTG 1 cut(s) 1298
BtsIMutI CAGTG 6 cut(s) 122, 644, 1298, 1426, 1455, 1503
BveI ACCTGC 2 cut(s) 285, 1486
Cac8I GCNNGC 5 cut(s) 716, 816, 995, 1497, 1572
CaiI CAGNNNCTG 1 cut(s) 46
CfoI GCGC 2 cut(s) 628, 1544
Cfr10I RCCGGY 2 cut(s) 1532, 1570
Cfr13I GGNCC 2 cut(s) 1427, 1535
Csp6I GTAC 3 cut(s) 1267, 1285, 1689
CviAII CATG 5 cut(s) 733, 777, 1103, 1469, 1720
CviQI GTAC 3 cut(s) 1267, 1285, 1689
DpnI GATC 8 cut(s) 285, 699, 724, 774, 786, 937, 1296, 1330
DpnII GATC 8 cut(s) 283, 697, 722, 772, 784, 935, 1294, 1328
DraIII CACNNNGTG 1 cut(s) 413
EaeI YGGCCR 1 cut(s) 1530
Eam1104I CTCTTC 4 cut(s) 126, 171, 1301, 1620
EarI CTCTTC 4 cut(s) 126, 171, 1301, 1620
Ecl136II GAGCTC 1 cut(s) 1009
Eco130I CCWWGG 3 cut(s) 189, 747, 1021
Eco147I AGGCCT 1 cut(s) 844
Eco24I GRGCYC 1 cut(s) 1011
Eco31I GGTCTC 2 cut(s) 797, 1439
Eco47I GGWCC 1 cut(s) 1535
Eco53kI GAGCTC 1 cut(s) 1009
Eco57I CTGAAG 2 cut(s) 195, 1259
EcoICRI GAGCTC 1 cut(s) 1009
EcoRII CCWGG 2 cut(s) 1042, 1429
EcoT14I CCWWGG 3 cut(s) 189, 747, 1021
EcoT38I GRGCYC 1 cut(s) 1011
ErhI CCWWGG 3 cut(s) 189, 747, 1021
FaeI CATG 5 cut(s) 736, 780, 1106, 1472, 1723
FaqI GGGAC 1 cut(s) 1435
FatI CATG 5 cut(s) 732, 776, 1102, 1468, 1719
FauI CCCGC 1 cut(s) 204
FbaI TGATCA 1 cut(s) 784
FokI GGATG 4 cut(s) 539, 662, 1223, 1522
FriOI GRGCYC 1 cut(s) 1011
FspBI CTAG 5 cut(s) 182, 477, 605, 1322, 1590
GlaI GCGC 2 cut(s) 627, 1543
HaeIII GGCC 4 cut(s) 844, 1429, 1495, 1532
HapII CCGG 3 cut(s) 1401, 1533, 1571
HhaI GCGC 2 cut(s) 628, 1544
Hin1II CATG 5 cut(s) 736, 780, 1106, 1472, 1723
Hin6I GCGC 2 cut(s) 626, 1542
HinP1I GCGC 2 cut(s) 626, 1542
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 1 cut(s) 1271
HinfI GANTC 4 cut(s) 111, 641, 1055, 1358
HpaII CCGG 3 cut(s) 1401, 1533, 1571
HphI GGTGA 6 cut(s) 335, 417, 425, 467, 980, 1367
Hpy166II GTNNAC 5 cut(s) 120, 556, 620, 1367, 1538
Hpy188I TCNGA 7 cut(s) 175, 543, 795, 940, 1067, 1239, 1516
Hpy188III TCNNGA 5 cut(s) 587, 665, 1144, 1262, 1332
Hpy8I GTNNAC 5 cut(s) 120, 556, 620, 1367, 1538
HpyAV CCTTC 4 cut(s) 688, 716, 1117, 1234
HpyCH4III ACNGT 4 cut(s) 256, 366, 1421, 1459
HpyF10VI GCNNNNNNNGC 8 cut(s) 277, 314, 317, 323, 399, 634, 1309, 1586
Hsp92II CATG 5 cut(s) 736, 780, 1106, 1472, 1723
HspAI GCGC 2 cut(s) 626, 1542
KroI GCCGGC 1 cut(s) 1570
KroNI GCCGGC 1 cut(s) 1572
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 8 cut(s) 283, 697, 722, 772, 784, 935, 1294, 1328
LguI GCTCTTC 2 cut(s) 126, 171
LmnI GCTCC 4 cut(s) 208, 1022, 1585, 1663
LweI GCATC 5 cut(s) 295, 517, 859, 1075, 1189
MaeI CTAG 5 cut(s) 182, 477, 605, 1322, 1590
MaeIII GTNAC 3 cut(s) 479, 982, 1595
MalI GATC 8 cut(s) 285, 699, 724, 774, 786, 937, 1296, 1330
MbiI CCGCTC 3 cut(s) 211, 1017, 1658
MboI GATC 8 cut(s) 283, 697, 722, 772, 784, 935, 1294, 1328
MfeI CAATTG 2 cut(s) 629, 825
MflI RGATCY 4 cut(s) 697, 722, 935, 1328
MhlI GDGCHC 1 cut(s) 1011
MlyI GAGTC 1 cut(s) 120
MroNI GCCGGC 1 cut(s) 1570
MseI TTAA 4 cut(s) 486, 564, 1028, 1215
MslI CAYNNNNRTG 2 cut(s) 737, 1508
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 3 cut(s) 1401, 1533, 1571
MspR9I CCNGG 2 cut(s) 1044, 1431
MunI CAATTG 2 cut(s) 629, 825
Mva1269I GAATGC 1 cut(s) 902
MvaI CCWGG 2 cut(s) 1044, 1431
MvnI CGCG 1 cut(s) 1542
MwoI GCNNNNNNNGC 8 cut(s) 277, 314, 317, 323, 399, 634, 1309, 1586
NaeI GCCGGC 1 cut(s) 1572
NdeII GATC 8 cut(s) 283, 697, 722, 772, 784, 935, 1294, 1328
NgoMIV GCCGGC 1 cut(s) 1570
NlaIII CATG 5 cut(s) 736, 780, 1106, 1472, 1723
NlaIV GGNNCC 2 cut(s) 363, 699
NmuCI GTSAC 2 cut(s) 479, 1595
NspI RCATGY 1 cut(s) 1723
NspV TTCGAA 1 cut(s) 1110
PceI AGGCCT 1 cut(s) 844
PciI ACATGT 1 cut(s) 1719
PciSI GCTCTTC 2 cut(s) 126, 171
PcsI WCGNNNNNNNCGW 1 cut(s) 715
PctI GAATGC 1 cut(s) 902
PdiI GCCGGC 1 cut(s) 1572
PfeI GAWTC 3 cut(s) 641, 1055, 1358
PflFI GACNNNGTC 1 cut(s) 110
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
PscI ACATGT 1 cut(s) 1719
Psp124BI GAGCTC 1 cut(s) 1011
Psp6I CCWGG 2 cut(s) 1042, 1429
PspGI CCWGG 2 cut(s) 1042, 1429
PspN4I GGNNCC 2 cut(s) 363, 699
PspPI GGNCC 2 cut(s) 1427, 1535
PstI CTGCAG 1 cut(s) 1305
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 4 cut(s) 697, 722, 935, 1328
PsyI GACNNNGTC 1 cut(s) 110
RsaI GTAC 3 cut(s) 1268, 1286, 1690
RsaNI GTAC 3 cut(s) 1267, 1285, 1689
RseI CAYNNNNRTG 2 cut(s) 737, 1508
SacI GAGCTC 1 cut(s) 1011
SapI GCTCTTC 2 cut(s) 126, 171
SaqAI TTAA 4 cut(s) 486, 564, 1028, 1215
Sau3AI GATC 8 cut(s) 283, 697, 722, 772, 784, 935, 1294, 1328
Sau96I GGNCC 2 cut(s) 1427, 1535
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 2 cut(s) 1044, 1431
SduI GDGCHC 1 cut(s) 1011
SfaNI GCATC 5 cut(s) 295, 517, 859, 1075, 1189
SfcI CTRYAG 2 cut(s) 1245, 1301
SfuI TTCGAA 1 cut(s) 1110
SinI GGWCC 1 cut(s) 1535
SmiMI CAYNNNNRTG 2 cut(s) 737, 1508
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 6 cut(s) 211, 311, 991, 1015, 1353, 1656
SspMI CTAG 5 cut(s) 182, 477, 605, 1322, 1590
SstI GAGCTC 1 cut(s) 1011
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 2 cut(s) 1042, 1429
StyI CCWWGG 3 cut(s) 189, 747, 1021
TaaI ACNGT 4 cut(s) 256, 366, 1421, 1459
TaqI TCGA 1 cut(s) 1110
TatI WGTACW 1 cut(s) 1266
TauI GCSGC 2 cut(s) 1017, 1658
TfiI GAWTC 3 cut(s) 641, 1055, 1358
Tru1I TTAA 4 cut(s) 486, 564, 1028, 1215
Tru9I TTAA 4 cut(s) 486, 564, 1028, 1215
TscAI CASTG 6 cut(s) 122, 651, 1305, 1426, 1462, 1510
TseFI GTSAC 2 cut(s) 479, 1595
Tsp45I GTSAC 2 cut(s) 479, 1595
TspDTI ATGAA 5 cut(s) 684, 876, 963, 969, 1091
TspRI CASTG 6 cut(s) 122, 651, 1305, 1426, 1462, 1510
Tth111I GACNNNGTC 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 1535
XapI RAATTY 4 cut(s) 200, 421, 883, 1174
XceI RCATGY 1 cut(s) 1723
XspI CTAG 5 cut(s) 182, 477, 605, 1322, 1590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.