MD02G1180500.v1.1

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013593
Physical Location & Seq
Forward (+)
16191446 .. 16195816
4371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1180500.v1.1.491

Sequence Viewer

Length: 1749 bp
ATGGCAAGTGCCTTCGCCCATGAGCGGTGTAGATTTGCATTAACCATGGTTGATGTGGAACAAGTTCTGGAAAGTGATGCACCAAGCTCTCTAATCGATCAGCTTGGTGACGCATTACTGGAACTAAATGCACACAATGATACTTCTGAGGACCAGGTTCAGTGGGAAGAAATTGAAGAGCATTTCCGCAACCTTGAGACAACACTGAGGAAGAATTTTGAAGAGCTTGAAGCCAAGGAGAAGAAATTTGCGGAGCAGGAAGCTGAAACCTGTGCTTCACTTGCGGAGAGAGAGGCAGCTGTTATTGCTAAAGAGCAAGACCTTTTGGATCGAGTGCAGGAGCTAAAAGATGCAGCTGTTACTGCTATTTTTGAGGCACGAGTCATTCACCAGCCAACAACTTCAAAACCTGTGGATGATGGGGATAACAAAAACAGCAAGGTAAGCAGCTCTCTTGGTGATACAAATTCATCAGAGGAGGATTTTCCTCATAAAACAGGTGATAATGCTGAGGGTATGGCTTCTGAGGTTAAGCCACGCCCAGCACTGATGAAATTTTGCGAGCAGATGGATGCAAAAGGGCTTCTGAATTATGCTACTGAGAATTATAAAAAACTAAATGTTATTCGTGAGGAACTTTCTGTTGCACTAGAAAGTGCAGGTGAACCAGCACGATTGGTGCTGGATTCACTGGAGGGGTTTTACCCTCCGGATGAGACTAACCAACCAGAGGGTAAGAAGGATGCTGCCCTTCAGGGCATGCGCAGATCCTGTCTAATGTTTATGGAAGCCATGACTGCCTTATTGGCAAAGGTTGACCCAGGTGCTGATCACCTTTTGAACCCTGACACCAAGCAGCAAGCCAAAGCAATTGCTGATGAGTGGAAGCCTAAGTTGGCTAGTGATGCTGCCAATGGAATTTCATTGGAAGCAGAGGCATTCTTGCAGCTCCTCGCTACTTTTAGGATTACTTCTGAGTTTGATGAAGACGAACTCTGCAAGTTTGTTCTTGCAGTTGCTCACCGCAGGCAGACACCTGAGCTCTGCCGCTCTCTTGGTTTAACACACAAAATGCCAGGTCTTGTTGAATCAATGGTCAGCGGTGGGAAACAAATTGATGCTGTACGGTTTATTCATGTCTTCCAGCTTACTGAAAGTTATCCCCTTGTGCCACTTCTAAAGACGTACTTGAAAGACTTGAGGAGAAATTCACAAGGGGACAGCACAGGAGATGGTACTGGTGCACAGGATGATGTTAATGCAAAAGAGCTTGGTGCACTGAAGGCCGTAATAAGGTGCGTGCAAGAGTTCAAGCTTGAGGCTGACTACCCCCTTGATCCGCTACAGAAAAGGGTTCATCAACTAGAGCGATCCAAAGCTGATAAGAAGAGAAGTAATGAATCTAAACGTCAACAGCAAAAGAAGCAAAAAATCAGTGGAAGATGGCGTGGTTACCGTGGCCCAGGTGTTGCTGCAACCTCTGCCCCGGCTGCTGCTAGGCACGGGCAACCTGTTTTTGGTGATAGGGCGGGATATGGTGGGATATTGGAGAGGTATGCTCAGCGTCCACTCGCATACGATTACCAAGTTCCCAGCCAGGCTGCTTATGTTCCACAAGCTAATGATCAAAGGTTATATTACTATCCCCAAGACGACAGAGTCAAACCAGCCCCGTTTAATGCTACTCCATCCAATTATGGCGGTTACGCTGGCAGTGGAATGCAGCCTCAGCACCAGCCGTACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

583

Amino Acids

64.73

Weight (kDa)

5.27

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 176 - 465 6e-102 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013593)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17030 FvH4_1g17030 FvH4_1g17030 FvH4_1g17030 FvH4_1g17030
malus_domestica MD02G1180500.v1.1
prunus_persica Prupe.6G225800_v2.0.a1
pyrus_communis pycom02g14640
rosa_chinensis RchiOBHm_Chr2g0107191
rosa_laevigata RLG00000017558
rosa_multiflora Rmu_co8250165.1_g000001
rosa_roxburghii Rroxscaffold_2G00136560
rosa_rugosa Rorug02G0140600
rosa_samantha Rh2AG191400 Rh2BG203400 Rh2CG196600 Rh2DG198700
rosa_wichuraiana Rw2G015130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AarI CACCTGC 1 cut(s) 650
AasI GACNNNNNNGTC 1 cut(s) 1658
Acc16I TGCGCA 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 650
AccBSI CCGCTC 2 cut(s) 25, 1050
AccIII TCCGGA 1 cut(s) 709
AclWI GGATC 4 cut(s) 336, 762, 1331, 1365
AcsI RAATTY 6 cut(s) 214, 245, 466, 554, 918, 1207
AcuI CTGAAG 2 cut(s) 737, 1301
AfaI GTAC 4 cut(s) 1125, 1187, 1237, 1742
AfiI CCNNNNNNNGG 4 cut(s) 24, 730, 1293, 1517
AflIII ACRYGT 1 cut(s) 1743
AgsI TTSAA 8 cut(s) 176, 221, 230, 405, 841, 1088, 1192, 1312
AjnI CCWGG 5 cut(s) 153, 820, 1075, 1462, 1596
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
Alw21I GWGCWC 3 cut(s) 1044, 1246, 1279
Alw26I GTCTC 2 cut(s) 191, 710
Alw44I GTGCAC 2 cut(s) 1242, 1275
AlwI GGATC 4 cut(s) 336, 762, 1331, 1365
AlwNI CAGNNNCTG 2 cut(s) 771, 827
Aor13HI TCCGGA 1 cut(s) 709
AoxI GGCC 2 cut(s) 1284, 1459
ApaLI GTGCAC 2 cut(s) 1242, 1275
ApoI RAATTY 6 cut(s) 214, 245, 466, 554, 918, 1207
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 765
AspS9I GGNCC 2 cut(s) 151, 1460
AsuC2I CCSGG 1 cut(s) 1487
AsuHPI GGTGA 8 cut(s) 119, 380, 470, 512, 674, 824, 1013, 1532
AvaII GGWCC 1 cut(s) 151
BaeGI GKGCMC 2 cut(s) 1246, 1279
BanII GRGCYC 1 cut(s) 1044
BauI CACGAG 1 cut(s) 378
BbsI GAAGAC 2 cut(s) 993, 1132
Bbv12I GWGCWC 3 cut(s) 1044, 1246, 1279
BbvCI CCTCAGC 2 cut(s) 510, 1728
BccI CCATC 5 cut(s) 413, 562, 1226, 1437, 1696
BceAI ACGGC 2 cut(s) 1271, 1723
BciT130I CCWGG 5 cut(s) 155, 822, 1077, 1464, 1598
BclI TGATCA 2 cut(s) 829, 1624
BcnI CCSGG 1 cut(s) 1487
BcoDI GTCTC 2 cut(s) 191, 710
BfaI CTAG 4 cut(s) 650, 900, 1364, 1497
BfmI CTRYAG 1 cut(s) 1343
BfuAI ACCTGC 1 cut(s) 650
BglI GCCNNNNNGGC 1 cut(s) 806
BlpI GCTNAGC 1 cut(s) 1560
Bme1390I CCNGG 6 cut(s) 155, 822, 1077, 1464, 1487, 1598
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 151, 1460
BmrFI CCNGG 6 cut(s) 155, 822, 1077, 1464, 1487, 1598
BmsI GCATC 6 cut(s) 67, 340, 562, 733, 895, 1108
BpiI GAAGAC 2 cut(s) 993, 1132
BplI GAGNNNNNCTC 2 cut(s) 1543, 1575
BpmI CTGGAG 1 cut(s) 713
Bpu10I CCTNAGC 3 cut(s) 510, 1038, 1728
Bpu1102I GCTNAGC 1 cut(s) 1560
BpuEI CTTGAG 3 cut(s) 215, 1219, 1337
BpuMI CCSGG 1 cut(s) 1487
Bsa29I ATCGAT 1 cut(s) 96
BsaJI CCNNGG 6 cut(s) 45, 234, 820, 1456, 1462, 1485
BsaWI WCCGGW 1 cut(s) 709
Bsc4I CCNNNNNNNGG 4 cut(s) 24, 730, 1293, 1517
Bse1I ACTGG 3 cut(s) 123, 696, 1243
BseAI TCCGGA 1 cut(s) 709
BseBI CCWGG 5 cut(s) 155, 822, 1077, 1464, 1598
BseCI ATCGAT 1 cut(s) 96
BseDI CCNNGG 6 cut(s) 45, 234, 820, 1456, 1462, 1485
BseGI GGATG 6 cut(s) 421, 577, 718, 748, 1255, 1688
BseLI CCNNNNNNNGG 4 cut(s) 24, 730, 1293, 1517
BseMII CTCAG 9 cut(s) 138, 197, 501, 516, 591, 966, 1029, 1574, 1742
BseNI ACTGG 3 cut(s) 123, 696, 1243
BseRI GAGGAG 3 cut(s) 491, 941, 1216
BseSI GKGCMC 2 cut(s) 1246, 1279
BseYI CCCAGC 2 cut(s) 541, 1592
BsgI GTGCAG 2 cut(s) 356, 678
BshFI GGCC 2 cut(s) 1286, 1461
BshVI ATCGAT 1 cut(s) 96
BsiHKAI GWGCWC 3 cut(s) 1044, 1246, 1279
BsiSI CCGG 2 cut(s) 710, 1487
BslFI GGGAC 1 cut(s) 1232
BslI CCNNNNNNNGG 4 cut(s) 24, 730, 1293, 1517
BsmAI GTCTC 2 cut(s) 191, 710
BsmFI GGGAC 1 cut(s) 1232
BsmI GAATGC 2 cut(s) 938, 1725
BsnI GGCC 2 cut(s) 1286, 1461
Bsp1286I GDGCHC 3 cut(s) 1044, 1246, 1279
Bsp13I TCCGGA 1 cut(s) 709
Bsp143I GATC 7 cut(s) 97, 328, 767, 829, 1336, 1370, 1624
Bsp1720I GCTNAGC 1 cut(s) 1560
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 2 cut(s) 1286, 1461
BspCNI CTCAG 9 cut(s) 139, 198, 502, 517, 592, 967, 1030, 1573, 1741
BspDI ATCGAT 1 cut(s) 96
BspEI TCCGGA 1 cut(s) 709
BspMI ACCTGC 1 cut(s) 650
BspPI GGATC 4 cut(s) 336, 762, 1331, 1365
BspQI GCTCTTC 2 cut(s) 171, 216
BsrBI CCGCTC 2 cut(s) 25, 1050
BsrI ACTGG 3 cut(s) 123, 696, 1243
BssECI CCNNGG 6 cut(s) 45, 234, 820, 1456, 1462, 1485
BssMI GATC 7 cut(s) 97, 328, 767, 829, 1336, 1370, 1624
BssSI CACGAG 1 cut(s) 378
BssT1I CCWWGG 2 cut(s) 45, 234
Bst2BI CACGAG 1 cut(s) 378
Bst2UI CCWGG 5 cut(s) 155, 822, 1077, 1464, 1598
Bst4CI ACNGT 2 cut(s) 1128, 1457
Bst6I CTCTTC 3 cut(s) 171, 216, 1382
BstAPI GCANNNNNTGC 1 cut(s) 1481
BstC8I GCNNGC 6 cut(s) 563, 761, 861, 1028, 1301, 1711
BstDSI CCRYGG 2 cut(s) 45, 1456
BstEII GGTNACC 1 cut(s) 1451
BstF5I GGATG 6 cut(s) 421, 577, 718, 748, 1255, 1688
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 7 cut(s) 100, 331, 770, 832, 1339, 1373, 1627
BstMAI GTCTC 2 cut(s) 191, 710
BstMBI GATC 7 cut(s) 97, 328, 767, 829, 1336, 1370, 1624
BstNI CCWGG 5 cut(s) 155, 822, 1077, 1464, 1598
BstNSI RCATGY 2 cut(s) 763, 1747
BstPI GGTNACC 1 cut(s) 1451
BstSCI CCNGG 6 cut(s) 153, 820, 1075, 1462, 1485, 1596
BstSFI CTRYAG 1 cut(s) 1343
BstSLI GKGCMC 2 cut(s) 1246, 1279
BstV2I GAAGAC 2 cut(s) 993, 1132
BstX2I RGATCY 1 cut(s) 767
BstYI RGATCY 1 cut(s) 767
Bsu15I ATCGAT 1 cut(s) 96
BsuRI GGCC 2 cut(s) 1286, 1461
BsuTUI ATCGAT 1 cut(s) 96
BtgI CCRYGG 2 cut(s) 45, 1456
BtsCI GGATG 6 cut(s) 421, 577, 718, 748, 1255, 1688
BtsI GCAGTG 1 cut(s) 1720
BtsIMutI CAGTG 7 cut(s) 167, 203, 545, 689, 1277, 1441, 1720
BveI ACCTGC 1 cut(s) 650
Cac8I GCNNGC 6 cut(s) 563, 761, 861, 1028, 1301, 1711
CaiI CAGNNNCTG 2 cut(s) 771, 827
CfoI GCGC 1 cut(s) 765
Cfr13I GGNCC 2 cut(s) 151, 1460
ClaI ATCGAT 1 cut(s) 96
CseI GACGC 2 cut(s) 119, 1553
CsiI ACCWGGT 1 cut(s) 153
Csp6I GTAC 4 cut(s) 1124, 1186, 1236, 1741
CspCI CAANNNNNGTGG 2 cut(s) 393, 428
CviAII CATG 6 cut(s) 20, 46, 760, 793, 1136, 1744
CviQI GTAC 4 cut(s) 1124, 1186, 1236, 1741
DpnI GATC 7 cut(s) 99, 330, 769, 831, 1338, 1372, 1626
DpnII GATC 7 cut(s) 97, 328, 767, 829, 1336, 1370, 1624
DrdI GACNNNNNNGTC 1 cut(s) 1658
DseDI GACNNNNNNGTC 1 cut(s) 1658
Eam1104I CTCTTC 3 cut(s) 171, 216, 1382
EarI CTCTTC 3 cut(s) 171, 216, 1382
Ecl136II GAGCTC 1 cut(s) 1042
Eco130I CCWWGG 2 cut(s) 45, 234
Eco24I GRGCYC 1 cut(s) 1044
Eco47I GGWCC 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 1042
Eco57I CTGAAG 2 cut(s) 737, 1301
Eco91I GGTNACC 1 cut(s) 1451
EcoICRI GAGCTC 1 cut(s) 1042
EcoO65I GGTNACC 1 cut(s) 1451
EcoRII CCWGG 5 cut(s) 153, 820, 1075, 1462, 1596
EcoT14I CCWWGG 2 cut(s) 45, 234
EcoT38I GRGCYC 1 cut(s) 1044
ErhI CCWWGG 2 cut(s) 45, 234
FaeI CATG 6 cut(s) 23, 49, 763, 796, 1139, 1747
FalI AAGNNNNNCTT 2 cut(s) 1172, 1204
FaqI GGGAC 1 cut(s) 1232
FatI CATG 6 cut(s) 19, 45, 759, 792, 1135, 1743
FauI CCCGC 1 cut(s) 1522
FbaI TGATCA 2 cut(s) 829, 1624
FokI GGATG 6 cut(s) 428, 584, 725, 755, 1262, 1675
FriOI GRGCYC 1 cut(s) 1044
FspBI CTAG 4 cut(s) 650, 900, 1364, 1497
FspI TGCGCA 1 cut(s) 764
GlaI GCGC 1 cut(s) 764
GsaI CCCAGC 2 cut(s) 545, 1596
GsuI CTGGAG 1 cut(s) 713
HaeIII GGCC 2 cut(s) 1286, 1461
HapII CCGG 2 cut(s) 710, 1487
HgaI GACGC 2 cut(s) 119, 1553
HhaI GCGC 1 cut(s) 765
Hin1II CATG 6 cut(s) 23, 49, 763, 796, 1139, 1747
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HincII GTYRAC 2 cut(s) 817, 1412
HindII GTYRAC 2 cut(s) 817, 1412
HindIII AAGCTT 1 cut(s) 1313
HinfI GANTC 5 cut(s) 381, 686, 1088, 1400, 1659
HpaII CCGG 2 cut(s) 710, 1487
HphI GGTGA 8 cut(s) 119, 380, 470, 512, 674, 824, 1013, 1532
Hpy166II GTNNAC 6 cut(s) 665, 817, 1244, 1277, 1412, 1568
Hpy188I TCNGA 5 cut(s) 148, 475, 526, 588, 976
Hpy188III TCNNGA 3 cut(s) 68, 629, 710
Hpy8I GTNNAC 6 cut(s) 665, 817, 1244, 1277, 1412, 1568
HpyAV CCTTC 4 cut(s) 22, 733, 761, 1276
HpyCH4III ACNGT 2 cut(s) 1128, 1457
HpyCH4IV ACGT 2 cut(s) 1184, 1408
HpySE526I ACGT 2 cut(s) 1184, 1408
Hsp92II CATG 6 cut(s) 23, 49, 763, 796, 1139, 1747
HspAI GCGC 1 cut(s) 763
Kpn2I TCCGGA 1 cut(s) 709
Ksp22I TGATCA 2 cut(s) 829, 1624
Kzo9I GATC 7 cut(s) 97, 328, 767, 829, 1336, 1370, 1624
LguI GCTCTTC 2 cut(s) 171, 216
LmnI GCTCC 3 cut(s) 253, 340, 954
LweI GCATC 6 cut(s) 67, 340, 562, 733, 895, 1108
MabI ACCWGGT 1 cut(s) 153
MaeI CTAG 4 cut(s) 650, 900, 1364, 1497
MaeII ACGT 2 cut(s) 1184, 1408
MaeIII GTNAC 4 cut(s) 107, 358, 1451, 1703
MalI GATC 7 cut(s) 99, 330, 769, 831, 1338, 1372, 1626
MbiI CCGCTC 2 cut(s) 25, 1050
MboI GATC 7 cut(s) 97, 328, 767, 829, 1336, 1370, 1624
MboII GAAGA 9 cut(s) 179, 188, 223, 233, 253, 998, 1132, 1399, 1452
MfeI CAATTG 1 cut(s) 870
MflI RGATCY 1 cut(s) 767
MhlI GDGCHC 3 cut(s) 1044, 1246, 1279
MlyI GAGTC 2 cut(s) 390, 1668
MroI TCCGGA 1 cut(s) 709
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 5 cut(s) 41, 531, 1061, 1257, 1677
MspA1I CMGCKG 3 cut(s) 299, 356, 1101
MspI CCGG 2 cut(s) 710, 1487
MspR9I CCNGG 6 cut(s) 155, 822, 1077, 1464, 1487, 1598
MunI CAATTG 1 cut(s) 870
Mva1269I GAATGC 2 cut(s) 938, 1725
MvaI CCWGG 5 cut(s) 155, 822, 1077, 1464, 1598
NciI CCSGG 1 cut(s) 1487
NcoI CCATGG 1 cut(s) 45
NdeII GATC 7 cut(s) 97, 328, 767, 829, 1336, 1370, 1624
NlaIII CATG 6 cut(s) 23, 49, 763, 796, 1139, 1747
NmuCI GTSAC 1 cut(s) 107
NsbI TGCGCA 1 cut(s) 764
NspI RCATGY 2 cut(s) 763, 1747
PaeI GCATGC 1 cut(s) 763
PaqCI CACCTGC 1 cut(s) 650
PciI ACATGT 1 cut(s) 1743
PciSI GCTCTTC 2 cut(s) 171, 216
PctI GAATGC 2 cut(s) 938, 1725
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 3 cut(s) 686, 1088, 1400
PflFI GACNNNGTC 1 cut(s) 1658
PleI GAGTC 2 cut(s) 389, 1667
PpsI GAGTC 2 cut(s) 389, 1667
PscI ACATGT 1 cut(s) 1743
PsiI TTATAA 1 cut(s) 609
Psp124BI GAGCTC 1 cut(s) 1044
Psp6I CCWGG 5 cut(s) 153, 820, 1075, 1462, 1596
PspEI GGTNACC 1 cut(s) 1451
PspFI CCCAGC 2 cut(s) 541, 1592
PspGI CCWGG 5 cut(s) 153, 820, 1075, 1462, 1596
PspPI GGNCC 2 cut(s) 151, 1460
PstNI CAGNNNCTG 2 cut(s) 771, 827
PsuI RGATCY 1 cut(s) 767
PsyI GACNNNGTC 1 cut(s) 1658
PvuII CAGCTG 2 cut(s) 299, 356
RsaI GTAC 4 cut(s) 1125, 1187, 1237, 1742
RsaNI GTAC 4 cut(s) 1124, 1186, 1236, 1741
SacI GAGCTC 1 cut(s) 1044
SapI GCTCTTC 2 cut(s) 171, 216
SaqAI TTAA 5 cut(s) 41, 531, 1061, 1257, 1677
Sau3AI GATC 7 cut(s) 97, 328, 767, 829, 1336, 1370, 1624
Sau96I GGNCC 2 cut(s) 151, 1460
SchI GAGTC 2 cut(s) 390, 1668
ScrFI CCNGG 6 cut(s) 155, 822, 1077, 1464, 1487, 1598
SduI GDGCHC 3 cut(s) 1044, 1246, 1279
SexAI ACCWGGT 1 cut(s) 153
SfaNI GCATC 6 cut(s) 67, 340, 562, 733, 895, 1108
SfcI CTRYAG 1 cut(s) 1343
SinI GGWCC 1 cut(s) 151
SmlI CTYRAG 3 cut(s) 194, 1198, 1316
SmoI CTYRAG 3 cut(s) 194, 1198, 1316
SphI GCATGC 1 cut(s) 763
SspMI CTAG 4 cut(s) 650, 900, 1364, 1497
SstI GAGCTC 1 cut(s) 1044
StyD4I CCNGG 6 cut(s) 153, 820, 1075, 1462, 1485, 1596
StyI CCWWGG 2 cut(s) 45, 234
TaaI ACNGT 2 cut(s) 1128, 1457
TaiI ACGT 2 cut(s) 1187, 1411
TaqI TCGA 2 cut(s) 96, 331
TauI GCSGC 1 cut(s) 1050
TfiI GAWTC 3 cut(s) 686, 1088, 1400
Tru1I TTAA 5 cut(s) 41, 531, 1061, 1257, 1677
Tru9I TTAA 5 cut(s) 41, 531, 1061, 1257, 1677
TscAI CASTG 7 cut(s) 167, 210, 552, 696, 1284, 1441, 1720
TseFI GTSAC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 7 cut(s) 459, 566, 912, 999, 1124, 1346, 1413
TspRI CASTG 7 cut(s) 167, 210, 552, 696, 1284, 1441, 1720
Tth111I GACNNNGTC 1 cut(s) 1658
VneI GTGCAC 2 cut(s) 1242, 1275
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 6 cut(s) 214, 245, 466, 554, 918, 1207
XceI RCATGY 2 cut(s) 763, 1747
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 4 cut(s) 650, 900, 1364, 1497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.