RLG00000017558

Belongs to the Frigida family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013593
Physical Location & Seq
Forward (+)
18257187 .. 18261262
4076 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017558

Sequence Viewer

Length: 1719 bp
ATGGCCGATGCAGAGCAAGTGGTGGCAAGTGATACATCACAGTGTTTGATAGATCAGCTTGGTATGGTATTACTGGAACTAAATGCTCATAATCATACTGCTGAGGACAGAGTTCAGTGGACAGAAATTGAAGAGCACTTCCGCAACCTTGAATTGACACTGAAGAAGAAATCTGAAGAGCTTGAAGCCAAGGAGAAGAAATTTGAGGAGCAGGAAGCTGAAACCTGTACTTTGCTTGTGGAGAGAGAAGCTACTGTTATTGCTAAAGAGCAAGCTCTGCTGGATCGGGTGCAGGAGCTAAAAGATGCAGCAGTCGCTGCTATTGCAGAGGCCCGAAACATTCACCAGTCAAGAAATTCGGAACCCATGAATGATGGGACTAACAAAGACAGCAAGGTAAGCAGCTCACTTGGTGATACAAATTCACCACAGGAAGATTTTCCAACTAAAAAAGGTGATAATGCTAAGGGTATGCCTAGTGACGTTAAGCCACGCCCAGAACTGAGGCAATTTTGTGAGCAGATGGATGCCAAAGGGCTTCTGAATTATATTGCTGAGAATCATAAAAAACTAAATGTTGCTCGTCAGGAACTTACTATTGCACTAGAAAGTGCAAGTGAACCAGCGCAATTGGTGCTGGATTCACTGGAGGGGTTTTACCCTCTTGATGAAACTAACCAACCAGAGGGTAAGAAGGATCCTGTCCTTCGGGGTAAGCGTAGATCCTGTCTCATGCTTATGGAATCCTTGACCACCTTAGCCTCAAAGGCTGATCGAGTTGCTGATCACCTTTTGAACCCTGAGACCAAGCAGCAAGCCAAAGCAATTGCTGATGAGTGGAGGCCTAAGTTGTTTAGTGGAGCTATTGACGCTGCCAATGGAATTTCATTGGAAGCAGAGGCATTCTTGCAGCTTCTTGCAACTTTTAGGATTGCTTCCGAGTTTGACGAAGAAGAACTCTGCAAGGTTGTTCTTGCAGCAGTTGCTCACCGAAGGCAGACACCTGAGCTCTGCCGTTCCCTTGGTTTAACACACAAAATGCCAGGTGTTGTTGAATCAATGATCAGAATTGGGAAACAAATTGACGCTGTGCATTTTATTCATGCCTTCCAGCTTACTGAAAGTTTTCCTCCTGTGCCACTTTTGAAGACGTATTTGAAAGAACTGAGGAGAAATTCACAAGGCGACAGCAATGGAGACACACAGGAGGATGTTAATGCAAAAGAGCTTTCTGGTCTGAAGGCGGTAGTAGGGTGTGTTCAAGACTACAAGCTTGAAGCTGAGTACCCCCTCGATCCACTGCAGAAAAGGCTTGTTCAACTAGAAAAATCCAAAGCTGACAGGAAGCGAAGTGGTGATTCTGGTAAACGCCAACAACCACCAAAGAAGCAAAAAGCCACTGGAAGGTGGCGTGGATACCGTGGTGGACCAGGTCTCGCCATTGCCTCATCCCCCACTGTTGGTAGGCACGGCCAACCTGTTTATGGTGAGAGGCCAGTATACACCGGGATGTCCGAGAGGTATGGTCTCACTGGTCCTCGCACCTACAATTATCAAGTTCCAAGCCAGCCGGCTTATGCTCCACAAGCTAGTGAACAAAGGCTATATTACTATCCCCCAGATGATAGAGCTGCATCAACCTCATATAACGCCGCTCCACCTATTTACGGCAGCTACATTGGTACTGGATTGCAGTCTCAGCACCAACCATACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

573

Amino Acids

63.73

Weight (kDa)

5.87

Isoelectric Point (pI)

55.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 162 - 451 9.6e-100 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013593)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17030 FvH4_1g17030 FvH4_1g17030 FvH4_1g17030 FvH4_1g17030
malus_domestica MD02G1180500.v1.1
prunus_persica Prupe.6G225800_v2.0.a1
pyrus_communis pycom02g14640
rosa_chinensis RchiOBHm_Chr2g0107191
rosa_laevigata RLG00000017558
rosa_multiflora Rmu_co8250165.1_g000001
rosa_roxburghii Rroxscaffold_2G00136560
rosa_rugosa Rorug02G0140600
rosa_samantha Rh2AG191400 Rh2BG203400 Rh2CG196600 Rh2DG198700
rosa_wichuraiana Rw2G015130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1655
AccI GTMKAC 1 cut(s) 1500
AciI CCGC 3 cut(s) 142, 1244, 1653
AclWI GGATC 5 cut(s) 291, 692, 705, 717, 1289
AcoI YGGCCR 2 cut(s) 3, 1471
AcsI RAATTY 5 cut(s) 200, 355, 421, 882, 1174
AcuI CTGAAG 3 cut(s) 182, 195, 1259
AdeI CACNNNGTG 1 cut(s) 413
AfaI GTAC 3 cut(s) 229, 1286, 1684
AfiI CCNNNNNNNGG 5 cut(s) 685, 1404, 1460, 1484, 1667
AflIII ACRYGT 1 cut(s) 1713
AjnI CCWGG 2 cut(s) 1042, 1429
Alw21I GWGCWC 2 cut(s) 138, 1011
Alw26I GTCTC 6 cut(s) 734, 797, 1191, 1439, 1532, 1701
AlwI GGATC 5 cut(s) 291, 692, 705, 717, 1289
AlwNI CAGNNNCTG 1 cut(s) 317
AoxI GGCC 5 cut(s) 3, 330, 842, 1471, 1493
ApeKI GCWGC 9 cut(s) 308, 317, 402, 811, 872, 910, 977, 1631, 1671
ApoI RAATTY 5 cut(s) 200, 355, 421, 882, 1174
Asp700I GAANNNNTTC 2 cut(s) 438, 1125
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 3 cut(s) 331, 1427, 1535
AsuC2I CCSGG 1 cut(s) 1507
AsuHPI GGTGA 8 cut(s) 335, 417, 425, 467, 779, 980, 1367, 1499
AvaII GGWCC 2 cut(s) 1427, 1535
BaeI ACNNNNGTAYC 2 cut(s) 1408, 1441
BamHI GGATCC 1 cut(s) 697
BanII GRGCYC 1 cut(s) 1011
BbsI GAAGAC 1 cut(s) 1154
Bbv12I GWGCWC 2 cut(s) 138, 1011
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 9 cut(s) 304, 320, 414, 823, 859, 922, 989, 1618, 1683
BccI CCATC 2 cut(s) 368, 517
BceAI ACGGC 3 cut(s) 999, 1486, 1684
BciT130I CCWGG 2 cut(s) 1044, 1431
BciVI GTATCC 1 cut(s) 1409
BclI TGATCA 2 cut(s) 784, 1062
BcnI CCSGG 1 cut(s) 1507
BcoDI GTCTC 6 cut(s) 734, 797, 1191, 1439, 1532, 1701
BfaI CTAG 4 cut(s) 477, 605, 1322, 1590
BfmI CTRYAG 1 cut(s) 1301
BfuI GTATCC 1 cut(s) 1409
BglI GCCNNNNNGGC 1 cut(s) 767
Bme1390I CCNGG 3 cut(s) 1044, 1431, 1507
Bme18I GGWCC 2 cut(s) 1427, 1535
BmgT120I GGNCC 3 cut(s) 331, 1427, 1535
BmiI GGNNCC 2 cut(s) 363, 699
BmrFI CCNGG 3 cut(s) 1044, 1431, 1507
BmsI GCATC 3 cut(s) 295, 517, 1643
BpiI GAAGAC 1 cut(s) 1154
BpmI CTGGAG 1 cut(s) 668
Bpu10I CCTNAGC 4 cut(s) 102, 465, 757, 1005
BpuMI CCSGG 1 cut(s) 1507
BsaI GGTCTC 3 cut(s) 797, 1439, 1532
BsaJI CCNNGG 3 cut(s) 189, 1021, 1420
Bsc4I CCNNNNNNNGG 5 cut(s) 685, 1404, 1460, 1484, 1667
Bse118I RCCGGY 1 cut(s) 1570
Bse1I ACTGG 7 cut(s) 78, 346, 651, 1405, 1496, 1537, 1690
Bse3DI GCAATG 2 cut(s) 1198, 1440
BseBI CCWGG 2 cut(s) 1044, 1431
BseDI CCNNGG 3 cut(s) 189, 1021, 1420
BseGI GGATG 4 cut(s) 532, 1216, 1448, 1515
BseLI CCNNNNNNNGG 5 cut(s) 685, 1404, 1460, 1484, 1667
BseMI GCAATG 2 cut(s) 1198, 1440
BseMII CTCAG 8 cut(s) 93, 494, 546, 792, 996, 1157, 1272, 1712
BseNI ACTGG 7 cut(s) 78, 346, 651, 1405, 1496, 1537, 1690
BseRI GAGGAG 2 cut(s) 221, 1183
BseXI GCAGC 9 cut(s) 304, 320, 414, 823, 859, 922, 989, 1618, 1683
BsgI GTGCAG 1 cut(s) 311
BshFI GGCC 5 cut(s) 5, 332, 844, 1473, 1495
BsiHKAI GWGCWC 2 cut(s) 138, 1011
BsiSI CCGG 2 cut(s) 1506, 1571
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 5 cut(s) 685, 1404, 1460, 1484, 1667
BsmAI GTCTC 6 cut(s) 734, 797, 1191, 1439, 1532, 1701
BsmFI GGGAC 1 cut(s) 391
BsmI GAATGC 1 cut(s) 902
BsnI GGCC 5 cut(s) 5, 332, 844, 1473, 1495
Bso31I GGTCTC 3 cut(s) 797, 1439, 1532
Bsp1286I GDGCHC 2 cut(s) 138, 1011
Bsp143I GATC 8 cut(s) 52, 283, 697, 722, 772, 784, 1062, 1294
BspACI CCGC 3 cut(s) 142, 1244, 1653
BspANI GGCC 5 cut(s) 5, 332, 844, 1473, 1495
BspCNI CTCAG 8 cut(s) 94, 495, 547, 793, 997, 1158, 1273, 1711
BspLI GGNNCC 2 cut(s) 363, 699
BspMAI CTGCAG 1 cut(s) 1305
BspPI GGATC 5 cut(s) 291, 692, 705, 717, 1289
BspQI GCTCTTC 2 cut(s) 126, 171
BspTNI GGTCTC 3 cut(s) 797, 1439, 1532
BsrBI CCGCTC 1 cut(s) 1655
BsrDI GCAATG 2 cut(s) 1198, 1440
BsrFI RCCGGY 1 cut(s) 1570
BsrI ACTGG 7 cut(s) 78, 346, 651, 1405, 1496, 1537, 1690
BssAI RCCGGY 1 cut(s) 1570
BssECI CCNNGG 3 cut(s) 189, 1021, 1420
BssMI GATC 8 cut(s) 52, 283, 697, 722, 772, 784, 1062, 1294
BssNAI GTATAC 1 cut(s) 1501
BssT1I CCWWGG 2 cut(s) 189, 1021
Bst1107I GTATAC 1 cut(s) 1501
Bst2UI CCWGG 2 cut(s) 1044, 1431
Bst4CI ACNGT 4 cut(s) 42, 256, 1421, 1459
Bst6I CTCTTC 2 cut(s) 126, 171
BstAPI GCANNNNNTGC 4 cut(s) 277, 317, 634, 983
BstC8I GCNNGC 4 cut(s) 273, 816, 1568, 1572
BstDSI CCRYGG 1 cut(s) 1420
BstF5I GGATG 4 cut(s) 532, 1216, 1448, 1515
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 8 cut(s) 55, 286, 700, 725, 775, 787, 1065, 1297
BstMAI GTCTC 6 cut(s) 734, 797, 1191, 1439, 1532, 1701
BstMBI GATC 8 cut(s) 52, 283, 697, 722, 772, 784, 1062, 1294
BstNI CCWGG 2 cut(s) 1044, 1431
BstNSI RCATGY 1 cut(s) 1717
BstSCI CCNGG 3 cut(s) 1042, 1429, 1505
BstSFI CTRYAG 1 cut(s) 1301
BstV1I GCAGC 9 cut(s) 304, 320, 414, 823, 859, 922, 989, 1618, 1683
BstV2I GAAGAC 1 cut(s) 1154
BstX2I RGATCY 2 cut(s) 697, 722
BstYI RGATCY 2 cut(s) 697, 722
BstZ17I GTATAC 1 cut(s) 1501
BsuI GTATCC 1 cut(s) 1409
BsuRI GGCC 5 cut(s) 5, 332, 844, 1473, 1495
BtgI CCRYGG 1 cut(s) 1420
BtsCI GGATG 4 cut(s) 532, 1216, 1448, 1515
BtsI GCAGTG 1 cut(s) 1298
BtsIMutI CAGTG 8 cut(s) 47, 122, 158, 644, 1298, 1398, 1455, 1530
Cac8I GCNNGC 4 cut(s) 273, 816, 1568, 1572
CaiI CAGNNNCTG 1 cut(s) 317
CfoI GCGC 1 cut(s) 628
Cfr10I RCCGGY 1 cut(s) 1570
Cfr13I GGNCC 3 cut(s) 331, 1427, 1535
CseI GACGC 2 cut(s) 878, 1094
CsiI ACCWGGT 1 cut(s) 1429
Csp6I GTAC 3 cut(s) 228, 1285, 1683
CviAII CATG 4 cut(s) 367, 733, 1103, 1714
CviQI GTAC 3 cut(s) 228, 1285, 1683
DpnI GATC 8 cut(s) 54, 285, 699, 724, 774, 786, 1064, 1296
DpnII GATC 8 cut(s) 52, 283, 697, 722, 772, 784, 1062, 1294
DraIII CACNNNGTG 1 cut(s) 413
EaeI YGGCCR 2 cut(s) 3, 1471
Eam1104I CTCTTC 2 cut(s) 126, 171
EarI CTCTTC 2 cut(s) 126, 171
Ecl136II GAGCTC 1 cut(s) 1009
Eco130I CCWWGG 2 cut(s) 189, 1021
Eco147I AGGCCT 1 cut(s) 844
Eco24I GRGCYC 1 cut(s) 1011
Eco31I GGTCTC 3 cut(s) 797, 1439, 1532
Eco47I GGWCC 2 cut(s) 1427, 1535
Eco53kI GAGCTC 1 cut(s) 1009
Eco57I CTGAAG 3 cut(s) 182, 195, 1259
EcoICRI GAGCTC 1 cut(s) 1009
EcoRII CCWGG 2 cut(s) 1042, 1429
EcoT14I CCWWGG 2 cut(s) 189, 1021
EcoT38I GRGCYC 1 cut(s) 1011
ErhI CCWWGG 2 cut(s) 189, 1021
FaeI CATG 4 cut(s) 370, 736, 1106, 1717
FaqI GGGAC 1 cut(s) 391
FatI CATG 4 cut(s) 366, 732, 1102, 1713
FbaI TGATCA 2 cut(s) 784, 1062
FblI GTMKAC 1 cut(s) 1500
FokI GGATG 4 cut(s) 539, 1223, 1435, 1522
FriOI GRGCYC 1 cut(s) 1011
FspBI CTAG 4 cut(s) 477, 605, 1322, 1590
GlaI GCGC 1 cut(s) 627
GsuI CTGGAG 1 cut(s) 668
HaeIII GGCC 5 cut(s) 5, 332, 844, 1473, 1495
HapII CCGG 2 cut(s) 1506, 1571
HgaI GACGC 2 cut(s) 878, 1094
HhaI GCGC 1 cut(s) 628
Hin1II CATG 4 cut(s) 370, 736, 1106, 1717
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HindIII AAGCTT 1 cut(s) 1271
HinfI GANTC 5 cut(s) 559, 641, 743, 1055, 1358
HpaII CCGG 2 cut(s) 1506, 1571
HphI GGTGA 8 cut(s) 335, 417, 425, 467, 779, 980, 1367, 1499
Hpy166II GTNNAC 6 cut(s) 120, 620, 1367, 1427, 1501, 1595
Hpy188I TCNGA 7 cut(s) 175, 361, 543, 940, 1067, 1239, 1516
Hpy188III TCNNGA 4 cut(s) 351, 587, 665, 1262
Hpy8I GTNNAC 6 cut(s) 120, 620, 1367, 1427, 1501, 1595
HpyAV CCTTC 6 cut(s) 688, 716, 987, 1117, 1234, 1398
HpyCH4III ACNGT 4 cut(s) 42, 256, 1421, 1459
HpyCH4IV ACGT 2 cut(s) 483, 1151
HpySE526I ACGT 2 cut(s) 483, 1151
Hsp92II CATG 4 cut(s) 370, 736, 1106, 1717
HspAI GCGC 1 cut(s) 626
KroI GCCGGC 1 cut(s) 1570
KroNI GCCGGC 1 cut(s) 1572
Ksp22I TGATCA 2 cut(s) 784, 1062
Kzo9I GATC 8 cut(s) 52, 283, 697, 722, 772, 784, 1062, 1294
LguI GCTCTTC 2 cut(s) 126, 171
LmnI GCTCC 5 cut(s) 208, 295, 860, 1585, 1660
Lsp1109I GCAGC 9 cut(s) 304, 320, 414, 823, 859, 922, 989, 1618, 1683
LweI GCATC 3 cut(s) 295, 517, 1643
MabI ACCWGGT 1 cut(s) 1429
MaeI CTAG 4 cut(s) 477, 605, 1322, 1590
MaeII ACGT 2 cut(s) 483, 1151
MaeIII GTNAC 1 cut(s) 479
MalI GATC 8 cut(s) 54, 285, 699, 724, 774, 786, 1064, 1296
MbiI CCGCTC 1 cut(s) 1655
MboI GATC 8 cut(s) 52, 283, 697, 722, 772, 784, 1062, 1294
MboII GAAGA 9 cut(s) 143, 175, 178, 188, 208, 446, 962, 965, 1159
MfeI CAATTG 2 cut(s) 629, 825
MflI RGATCY 2 cut(s) 697, 722
MhlI GDGCHC 2 cut(s) 138, 1011
MmeI TCCRAC 1 cut(s) 467
MroNI GCCGGC 1 cut(s) 1570
MroXI GAANNNNTTC 2 cut(s) 438, 1125
MseI TTAA 3 cut(s) 486, 1028, 1215
MslI CAYNNNNRTG 3 cut(s) 40, 737, 1508
MspI CCGG 2 cut(s) 1506, 1571
MspR9I CCNGG 3 cut(s) 1044, 1431, 1507
MunI CAATTG 2 cut(s) 629, 825
Mva1269I GAATGC 1 cut(s) 902
MvaI CCWGG 2 cut(s) 1044, 1431
NaeI GCCGGC 1 cut(s) 1572
NciI CCSGG 1 cut(s) 1507
NdeII GATC 8 cut(s) 52, 283, 697, 722, 772, 784, 1062, 1294
NgoMIV GCCGGC 1 cut(s) 1570
NlaIII CATG 4 cut(s) 370, 736, 1106, 1717
NlaIV GGNNCC 2 cut(s) 363, 699
NmuCI GTSAC 1 cut(s) 479
NspI RCATGY 1 cut(s) 1717
PceI AGGCCT 1 cut(s) 844
PciI ACATGT 1 cut(s) 1713
PciSI GCTCTTC 2 cut(s) 126, 171
PcsI WCGNNNNNNNCGW 1 cut(s) 715
PctI GAATGC 1 cut(s) 902
PdiI GCCGGC 1 cut(s) 1572
PdmI GAANNNNTTC 2 cut(s) 438, 1125
PfeI GAWTC 5 cut(s) 559, 641, 743, 1055, 1358
PflFI GACNNNGTC 1 cut(s) 1431
PscI ACATGT 1 cut(s) 1713
Psp124BI GAGCTC 1 cut(s) 1011
Psp6I CCWGG 2 cut(s) 1042, 1429
PspGI CCWGG 2 cut(s) 1042, 1429
PspN4I GGNNCC 2 cut(s) 363, 699
PspPI GGNCC 3 cut(s) 331, 1427, 1535
PsrI GAACNNNNNNTAC 2 cut(s) 1242, 1274
PstI CTGCAG 1 cut(s) 1305
PstNI CAGNNNCTG 1 cut(s) 317
PsuI RGATCY 2 cut(s) 697, 722
PsyI GACNNNGTC 1 cut(s) 1431
RsaI GTAC 3 cut(s) 229, 1286, 1684
RsaNI GTAC 3 cut(s) 228, 1285, 1683
RseI CAYNNNNRTG 3 cut(s) 40, 737, 1508
SacI GAGCTC 1 cut(s) 1011
SapI GCTCTTC 2 cut(s) 126, 171
SaqAI TTAA 3 cut(s) 486, 1028, 1215
Sau3AI GATC 8 cut(s) 52, 283, 697, 722, 772, 784, 1062, 1294
Sau96I GGNCC 3 cut(s) 331, 1427, 1535
ScrFI CCNGG 3 cut(s) 1044, 1431, 1507
SduI GDGCHC 2 cut(s) 138, 1011
SexAI ACCWGGT 1 cut(s) 1429
SfaNI GCATC 3 cut(s) 295, 517, 1643
SfcI CTRYAG 1 cut(s) 1301
SinI GGWCC 2 cut(s) 1427, 1535
SmiMI CAYNNNNRTG 3 cut(s) 40, 737, 1508
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 3 cut(s) 142, 1244, 1653
SspMI CTAG 4 cut(s) 477, 605, 1322, 1590
SstI GAGCTC 1 cut(s) 1011
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 3 cut(s) 1042, 1429, 1505
StyI CCWWGG 2 cut(s) 189, 1021
TaaI ACNGT 4 cut(s) 42, 256, 1421, 1459
TaiI ACGT 2 cut(s) 486, 1154
TaqI TCGA 2 cut(s) 775, 1293
TatI WGTACW 1 cut(s) 227
TauI GCSGC 1 cut(s) 1655
TfiI GAWTC 5 cut(s) 559, 641, 743, 1055, 1358
Tru1I TTAA 3 cut(s) 486, 1028, 1215
Tru9I TTAA 3 cut(s) 486, 1028, 1215
TscAI CASTG 8 cut(s) 47, 122, 165, 651, 1305, 1405, 1462, 1537
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 9 cut(s) 308, 317, 402, 811, 872, 910, 977, 1631, 1671
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 4 cut(s) 383, 684, 876, 1091
TspRI CASTG 8 cut(s) 47, 122, 165, 651, 1305, 1405, 1462, 1537
Tth111I GACNNNGTC 1 cut(s) 1431
VpaK11BI GGWCC 2 cut(s) 1427, 1535
XapI RAATTY 5 cut(s) 200, 355, 421, 882, 1174
XceI RCATGY 1 cut(s) 1717
XcmI CCANNNNNNNNNTGG 1 cut(s) 1481
XmiI GTMKAC 1 cut(s) 1500
XmnI GAANNNNTTC 2 cut(s) 438, 1125
XspI CTAG 4 cut(s) 477, 605, 1322, 1590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.