Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0012821

Physical Location & Seq

Genomic Coordinates Reverse (-)

12424144 .. 12430071

Gene Structure 5928 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g20220.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGGCTTCTATCAACCTCTATTGCTCCCCAATGGCTCTCTTCCCTTCTCAGAGGAACAATGGTTTATGTACTGGGACTTTGGTTAAGCCAAATGGGTGTGTGTCTGATTTTGTTGGGCCGAGTTTGACGCGAAAGTTGTGCTTTAGTTTGAGGGGTGATTTTCAGGCAAGAGCTACGTTGATATCTGGAGAGGATGATCTCCTGTCTCACACTAATGGGGTTGAAGCGCAACATGATGCTTTGAGTTTTGGAACGCTTGCAGCTGATATGGCCCCTTTGAGTAGCGGTTTCTCTTCTGATAATGATGAATATGATCTGGATTATCCTATTCAAGGATTTTCTTCTATTGCGGAAGCCATTGAAGATATTCGTCAAGGCAAGATGGTAATTGTTGTGGATGATGAAGATAGAGAAAATGAGGGAGATCTTATAATGGCAGCATCCTTGGCAACACCAGAAGCTATGGCTTTTATTGTCAAGCACGGAACCGGAATTGTTTGTGTGAGCATGAAAGGCGAAGACTTAGAAAGGTTGCAACTTCCGTTGATGGTGACACAGAATGAAGAAAAACTTTGTACAGCATTCACTGTCTCAGTGGATGCAAAACATGGTACGACAACTGGTGTTTCAGCTCGCGATAGAGCTGCGACAGTTTTGGCTCTTGCATCAAGGGATTCAAAACCTGAAGATTTCAACCGACCAGGCCATATCTTTCCCCTAAAGTACAGGGAGGGAGGAGTTCTAAAAAGAGCTGGGCACACAGAAGCTTCAGTTGATCTTGCAATGTTGGCTGGGCTAGATCCGGTAGCAGTTTTGTGTGAAATTGTGGATGATGACGGTTCTATGGCTAGATTACCGAGGCTTCGTCAATTTGCAAAGGCAGAAAACTTGAAAATTATTTCTATTGCTGATTTGATAAGATATAGGAGAAAAAGGGACAAGTTGGTGGAGCGGGCTGGAGATGCACGGATACCAACAATGTGGGGGCCATTTGAAGCCTACTGTTATAGGTCAATGCTTGATGGGATCGAGCATATTGCTATGGTCAAAGGTGATATTGGAGACGGGCAAGATATACTTGTGAGAGTACATTCAGAGTGCCTCACTGGCGACATCTTTGGTTCGGCCAGATGTGATTGTGGAAACCAACTGGCTCTTGCTATGAAACAAATTGAAGCAGCTGGTAGGGGTGTTTTGGTGTATCTCCGAGGACATGAAGGTAGAGGAATTGGTTTAGGCCACAAGCTCCGAGCTTATAACCTGCAGGATGATGGGCGTGACACTGTTGAAGCCAATGAGGAGCTCGGACTACCTGTTGATTCTCGAGAGTATGGAATAGGTGCACAGATCCTACGAGATCTAGGCGTTCGTACAATGAAACTTATGACAAACAATCCTGCAAAGTATAGCGGACTGAAAGGTTACGGTTTGGCAGTTGCTGGGAGGGTCCCGTTATTGACACCAATAACTAAGGAGAACAAGAGATATTTGGAGACTAAACGTACAAAAATGGGGCACGTCTATGGCTCCAATCTCAATGGTCATGTAGATAGCACCAGTGCCGAAAGCAGTTCAAGCATCGGCAACCCATCTAATGGTGTCTCTGAGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006766 GO:0006767 GO:0006771 GO:0006807 GO:0008150 GO:0008152 GO:0008686 GO:0009058 GO:0009110 GO:0009231 GO:0009507 GO:0009536 GO:0009987 GO:0016829 GO:0016830 GO:0017144 GO:0018130 GO:0034641 GO:0042364 GO:0042726 GO:0042727 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00740 ko00790 ko01100 ko01110 map00740 map00790 map01100 map01110

Pfam Domains

Protein Families

DHBP_synthase GTP_cyclohydro2

Protein Analysis

538

Amino Acids

58.12

Weight (kDa)

5.57

Isoelectric Point (pI)

30.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHBP_synthase	PF00926	118 - 308	5.1e-88	3,4-dihydroxy-2-butanone 4-phosphate synthase
GTP_cyclohydro2	PF00925	318 - 484	9.9e-72	GTP cyclohydrolase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64300
fragaria_vesca	FvH4_1g20220 FvH4_1g20220 FvH4_1g20220 FvH4_1g20220
malus_domestica	MD01G1036500.v1.1
prunus_persica	Prupe.6G193700_v2.0.a1 Prupe.6G193700_v2.0.a1
pyrus_communis	pycom01g06320 pycom15g28070
rosa_chinensis	RchiOBHm_Chr2g0112291
rosa_laevigata	RLG00000017969
rosa_roxburghii	Rroxscaffold_2G00131530
rosa_samantha	Rh2AG232400 Rh2BG245800 Rh2CG236600 Rh2DG240100
rosa_wichuraiana	Rw2G018030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	431, 1257
Acc36I	ACCTGC	1 cut(s)	1269
AccB7I	CCANNNNNTGG	1 cut(s)	1595
AccBSI	CCGCTC	1 cut(s)	952
AccII	CGCG	2 cut(s)	130, 636
AciI	CCGC	4 cut(s)	285, 350, 952, 1410
AclWI	GGATC	3 cut(s)	794, 1034, 1342
AcoI	YGGCCR	1 cut(s)	1125
AcuI	CTGAAG	2 cut(s)	705, 753
AfaI	GTAC	7 cut(s)	70, 577, 613, 725, 1089, 1372, 1504
AfiI	CCNNNNNNNGG	2 cut(s)	332, 1595
AjiI	CACGTC	1 cut(s)	1519
AjnI	CCWGG	1 cut(s)	700
Alw21I	GWGCWC	2 cut(s)	1305, 1345
Alw26I	GTCTC	6 cut(s)	210, 595, 1056, 1487, 1601, 1606
Alw44I	GTGCAC	1 cut(s)	1341
AlwI	GGATC	3 cut(s)	794, 1034, 1342
AlwNI	CAGNNNCTG	1 cut(s)	1439
Ama87I	CYCGRG	1 cut(s)	1323
AoxI	GGCC	6 cut(s)	116, 270, 703, 986, 1125, 1237
ApaLI	GTGCAC	1 cut(s)	1341
ApeKI	GCWGC	4 cut(s)	260, 437, 644, 1178
Asp700I	GAANNNNTTC	1 cut(s)	366
AspLEI	GCGC	1 cut(s)	229
AspS9I	GGNCC	4 cut(s)	116, 271, 986, 1447
AsuHPI	GGTGA	3 cut(s)	167, 562, 1064
AvaI	CYCGRG	1 cut(s)	1323
AvaII	GGWCC	1 cut(s)	1447
BaeGI	GKGCMC	3 cut(s)	759, 1345, 1518
BanII	GRGCYC	1 cut(s)	1305
BbsI	GAAGAC	1 cut(s)	525
Bbv12I	GWGCWC	2 cut(s)	1305, 1345
BbvI	GCAGC	4 cut(s)	272, 449, 631, 1190
BccI	CCATC	5 cut(s)	376, 541, 1016, 1265, 1597
BcgI	CGANNNNNNTGC	6 cut(s)	120, 154, 626, 660, 1019, 1053
BciT130I	CCWGG	1 cut(s)	702
BciVI	GTATCC	1 cut(s)	963
BcoDI	GTCTC	6 cut(s)	210, 595, 1056, 1487, 1601, 1606
BfaI	CTAG	3 cut(s)	797, 849, 1361
BfmI	CTRYAG	1 cut(s)	1262
BfuAI	ACCTGC	1 cut(s)	1269
BfuI	GTATCC	1 cut(s)	963
BglI	GCCNNNNNGGC	1 cut(s)	1107
BglII	AGATCT	2 cut(s)	424, 1357
BisI	GCNGC	4 cut(s)	261, 438, 645, 1179
BlsI	GCNGC	4 cut(s)	262, 439, 646, 1180
Bme1390I	CCNGG	1 cut(s)	702
Bme18I	GGWCC	1 cut(s)	1447
BmeT110I	CYCGRG	1 cut(s)	1323
BmgBI	CACGTC	1 cut(s)	1519
BmgT120I	GGNCC	4 cut(s)	116, 271, 986, 1447
BmiI	GGNNCC	6 cut(s)	273, 487, 987, 1448, 1449, 1528
BmrFI	CCNGG	1 cut(s)	702
BmrI	ACTGGG	1 cut(s)	81
BmsI	GCATC	6 cut(s)	226, 449, 589, 674, 952, 1587
BmuI	ACTGGG	1 cut(s)	81
BpiI	GAAGAC	1 cut(s)	525
BpmI	CTGGAG	2 cut(s)	207, 978
BsaJI	CCNNGG	3 cut(s)	444, 857, 1207
BsaWI	WCCGGW	2 cut(s)	488, 802
Bsc4I	CCNNNNNNNGG	2 cut(s)	332, 1595
Bse1I	ACTGG	5 cut(s)	76, 625, 1111, 1155, 1557
Bse3DI	GCAATG	1 cut(s)	789
BseBI	CCWGG	1 cut(s)	702
BseDI	CCNNGG	3 cut(s)	444, 857, 1207
BseGI	GGATG	6 cut(s)	199, 403, 440, 604, 835, 1273
BseLI	CCNNNNNNNGG	2 cut(s)	332, 1595
BseMI	GCAATG	1 cut(s)	789
BseMII	CTCAG	3 cut(s)	62, 606, 1596
BseNI	ACTGG	5 cut(s)	76, 625, 1111, 1155, 1557
BseRI	GAGGAG	2 cut(s)	750, 1313
BseSI	GKGCMC	3 cut(s)	759, 1345, 1518
BseXI	GCAGC	4 cut(s)	272, 449, 631, 1190
BseYI	CCCAGC	3 cut(s)	752, 791, 1439
Bsh1236I	CGCG	2 cut(s)	130, 636
BshFI	GGCC	6 cut(s)	118, 272, 705, 988, 1127, 1239
BsiHKAI	GWGCWC	2 cut(s)	1305, 1345
BsiHKCI	CYCGRG	1 cut(s)	1323
BsiSI	CCGG	2 cut(s)	489, 803
BslFI	GGGAC	3 cut(s)	88, 950, 1433
BslI	CCNNNNNNNGG	2 cut(s)	332, 1595
BsmAI	GTCTC	6 cut(s)	210, 595, 1056, 1487, 1601, 1606
BsmBI	CGTCTC	1 cut(s)	1056
BsmFI	GGGAC	3 cut(s)	88, 950, 1433
BsmI	GAATGC	1 cut(s)	581
BsnI	GGCC	6 cut(s)	118, 272, 705, 988, 1127, 1239
BsoBI	CYCGRG	1 cut(s)	1323
Bsp1286I	GDGCHC	4 cut(s)	759, 1305, 1345, 1518
Bsp1407I	TGTACA	1 cut(s)	575
Bsp143I	GATC	8 cut(s)	196, 313, 424, 775, 799, 1026, 1347, 1357
Bsp68I	TCGCGA	1 cut(s)	636
BspACI	CCGC	4 cut(s)	285, 350, 952, 1410
BspANI	GGCC	6 cut(s)	118, 272, 705, 988, 1127, 1239
BspCNI	CTCAG	3 cut(s)	61, 605, 1597
BspFNI	CGCG	2 cut(s)	130, 636
BspLI	GGNNCC	6 cut(s)	273, 487, 987, 1448, 1449, 1528
BspMAI	CTGCAG	1 cut(s)	1266
BspMI	ACCTGC	1 cut(s)	1269
BspPI	GGATC	3 cut(s)	794, 1034, 1342
BsrBI	CCGCTC	1 cut(s)	952
BsrDI	GCAATG	1 cut(s)	789
BsrGI	TGTACA	1 cut(s)	575
BsrI	ACTGG	5 cut(s)	76, 625, 1111, 1155, 1557
BssECI	CCNNGG	3 cut(s)	444, 857, 1207
BssMI	GATC	8 cut(s)	196, 313, 424, 775, 799, 1026, 1347, 1357
BssT1I	CCWWGG	1 cut(s)	444
Bst2UI	CCWGG	1 cut(s)	702
Bst4CI	ACNGT	6 cut(s)	589, 652, 839, 1004, 1285, 1427
Bst6I	CTCTTC	2 cut(s)	44, 298
BstAUI	TGTACA	1 cut(s)	575
BstC8I	GCNNGC	3 cut(s)	258, 634, 954
BstDEI	CTNAG	5 cut(s)	48, 523, 592, 1470, 1605
BstENI	CCTNNNNNAGG	1 cut(s)	330
BstF5I	GGATG	6 cut(s)	199, 403, 440, 604, 835, 1273
BstFNI	CGCG	2 cut(s)	130, 636
BstHHI	GCGC	1 cut(s)	229
BstKTI	GATC	8 cut(s)	199, 316, 427, 778, 802, 1029, 1350, 1360
BstMAI	GTCTC	6 cut(s)	210, 595, 1056, 1487, 1601, 1606
BstMBI	GATC	8 cut(s)	196, 313, 424, 775, 799, 1026, 1347, 1357
BstMWI	GCNNNNNNNGC	7 cut(s)	269, 446, 513, 788, 962, 1107, 1575
BstNI	CCWGG	1 cut(s)	702
BstSCI	CCNGG	1 cut(s)	700
BstSFI	CTRYAG	1 cut(s)	1262
BstSLI	GKGCMC	3 cut(s)	759, 1345, 1518
BstUI	CGCG	2 cut(s)	130, 636
BstV1I	GCAGC	4 cut(s)	272, 449, 631, 1190
BstV2I	GAAGAC	1 cut(s)	525
BstX2I	RGATCY	4 cut(s)	424, 799, 1347, 1357
BstXI	CCANNNNNNTGG	1 cut(s)	981
BstYI	RGATCY	4 cut(s)	424, 799, 1347, 1357
BsuI	GTATCC	1 cut(s)	963
BsuRI	GGCC	6 cut(s)	118, 272, 705, 988, 1127, 1239
BtrI	CACGTC	1 cut(s)	1519
BtsCI	GGATG	6 cut(s)	199, 403, 440, 604, 835, 1273
BtsIMutI	CAGTG	5 cut(s)	585, 600, 1104, 1281, 1564
BtuMI	TCGCGA	1 cut(s)	636
BveI	ACCTGC	1 cut(s)	1269
Cac8I	GCNNGC	3 cut(s)	258, 634, 954
CaiI	CAGNNNCTG	1 cut(s)	1439
CfoI	GCGC	1 cut(s)	229
Cfr13I	GGNCC	4 cut(s)	116, 271, 986, 1447
CseI	GACGC	1 cut(s)	136
Csp6I	GTAC	7 cut(s)	69, 576, 612, 724, 1088, 1371, 1503
CviAII	CATG	6 cut(s)	233, 508, 608, 1214, 1544, 1611
CviQI	GTAC	7 cut(s)	69, 576, 612, 724, 1088, 1371, 1503
DdeI	CTNAG	5 cut(s)	48, 523, 592, 1470, 1605
DpnI	GATC	8 cut(s)	198, 315, 426, 777, 801, 1028, 1349, 1359
DpnII	GATC	8 cut(s)	196, 313, 424, 775, 799, 1026, 1347, 1357
EaeI	YGGCCR	1 cut(s)	1125
Eam1104I	CTCTTC	2 cut(s)	44, 298
EarI	CTCTTC	2 cut(s)	44, 298
Ecl136II	GAGCTC	1 cut(s)	1303
Eco130I	CCWWGG	1 cut(s)	444
Eco24I	GRGCYC	1 cut(s)	1305
Eco32I	GATATC	1 cut(s)	183
Eco47I	GGWCC	1 cut(s)	1447
Eco53kI	GAGCTC	1 cut(s)	1303
Eco57I	CTGAAG	2 cut(s)	705, 753
Eco88I	CYCGRG	1 cut(s)	1323
EcoICRI	GAGCTC	1 cut(s)	1303
EcoNI	CCTNNNNNAGG	1 cut(s)	330
EcoO109I	RGGNCCY	1 cut(s)	1447
EcoRII	CCWGG	1 cut(s)	700
EcoRV	GATATC	1 cut(s)	183
EcoT14I	CCWWGG	1 cut(s)	444
EcoT38I	GRGCYC	1 cut(s)	1305
ErhI	CCWWGG	1 cut(s)	444
Esp3I	CGTCTC	1 cut(s)	1056
FaeI	CATG	6 cut(s)	236, 511, 611, 1217, 1547, 1614
FalI	AAGNNNNNCTT	6 cut(s)	125, 157, 555, 587, 1062, 1094
FaqI	GGGAC	3 cut(s)	88, 950, 1433
FatI	CATG	6 cut(s)	232, 507, 607, 1213, 1543, 1610
FauI	CCCGC	1 cut(s)	945
Fnu4HI	GCNGC	4 cut(s)	261, 438, 645, 1179
FokI	GGATG	6 cut(s)	206, 410, 427, 611, 842, 1280
FriOI	GRGCYC	1 cut(s)	1305
Fsp4HI	GCNGC	4 cut(s)	261, 438, 645, 1179
FspBI	CTAG	3 cut(s)	797, 849, 1361
GlaI	GCGC	1 cut(s)	228
GluI	GCNGC	4 cut(s)	261, 438, 645, 1179
GsaI	CCCAGC	3 cut(s)	756, 795, 1443
GsuI	CTGGAG	2 cut(s)	207, 978
HaeIII	GGCC	6 cut(s)	118, 272, 705, 988, 1127, 1239
HapII	CCGG	2 cut(s)	489, 803
HgaI	GACGC	1 cut(s)	136
HhaI	GCGC	1 cut(s)	229
Hin1II	CATG	6 cut(s)	236, 511, 611, 1217, 1547, 1614
Hin6I	GCGC	1 cut(s)	227
HinP1I	GCGC	1 cut(s)	227
HindIII	AAGCTT	1 cut(s)	765
HinfI	GANTC	2 cut(s)	674, 1319
HpaII	CCGG	2 cut(s)	489, 803
HphI	GGTGA	3 cut(s)	167, 562, 1064
Hpy166II	GTNNAC	1 cut(s)	1343
Hpy188I	TCNGA	8 cut(s)	51, 106, 298, 1096, 1208, 1250, 1307, 1606
Hpy188III	TCNNGA	5 cut(s)	186, 317, 635, 1323, 1325
Hpy8I	GTNNAC	1 cut(s)	1343
HpyAV	CCTTC	2 cut(s)	54, 1211
HpyCH4III	ACNGT	6 cut(s)	589, 652, 839, 1004, 1285, 1427
HpyCH4IV	ACGT	3 cut(s)	176, 1501, 1518
HpyF10VI	GCNNNNNNNGC	7 cut(s)	269, 446, 513, 788, 962, 1107, 1575
HpyF3I	CTNAG	5 cut(s)	48, 523, 592, 1470, 1605
HpySE526I	ACGT	3 cut(s)	176, 1501, 1518
Hsp92II	CATG	6 cut(s)	236, 511, 611, 1217, 1547, 1614
HspAI	GCGC	1 cut(s)	227
KflI	GGGWCCC	1 cut(s)	1447
Kzo9I	GATC	8 cut(s)	196, 313, 424, 775, 799, 1026, 1347, 1357
LmnI	GCTCC	5 cut(s)	29, 949, 1251, 1300, 1532
Lsp1109I	GCAGC	4 cut(s)	272, 449, 631, 1190
LweI	GCATC	6 cut(s)	226, 449, 589, 674, 952, 1587
MaeI	CTAG	3 cut(s)	797, 849, 1361
MaeII	ACGT	3 cut(s)	176, 1501, 1518
MaeIII	GTNAC	3 cut(s)	550, 1277, 1421
MalI	GATC	8 cut(s)	198, 315, 426, 777, 801, 1028, 1349, 1359
MbiI	CCGCTC	1 cut(s)	952
MboI	GATC	8 cut(s)	196, 313, 424, 775, 799, 1026, 1347, 1357
MboII	GAAGA	8 cut(s)	31, 285, 333, 374, 416, 530, 575, 698
MflI	RGATCY	4 cut(s)	424, 799, 1347, 1357
MhlI	GDGCHC	4 cut(s)	759, 1305, 1345, 1518
MluCI	AATT	7 cut(s)	387, 492, 822, 869, 894, 1170, 1227
MroXI	GAANNNNTTC	1 cut(s)	366
MseI	TTAA	1 cut(s)	84
MslI	CAYNNNNRTG	2 cut(s)	213, 1521
MspA1I	CMGCKG	2 cut(s)	263, 1181
MspI	CCGG	2 cut(s)	489, 803
MspR9I	CCNGG	1 cut(s)	702
Mva1269I	GAATGC	1 cut(s)	581
MvaI	CCWGG	1 cut(s)	702
MvnI	CGCG	2 cut(s)	130, 636
MwoI	GCNNNNNNNGC	7 cut(s)	269, 446, 513, 788, 962, 1107, 1575
NdeII	GATC	8 cut(s)	196, 313, 424, 775, 799, 1026, 1347, 1357
NlaIII	CATG	6 cut(s)	236, 511, 611, 1217, 1547, 1614
NlaIV	GGNNCC	6 cut(s)	273, 487, 987, 1448, 1449, 1528
NmeAIII	GCCGAG	1 cut(s)	144
NmuCI	GTSAC	2 cut(s)	550, 1277
NruI	TCGCGA	1 cut(s)	636
PaeR7I	CTCGAG	1 cut(s)	1323
PctI	GAATGC	1 cut(s)	581
PdmI	GAANNNNTTC	1 cut(s)	366
PfeI	GAWTC	2 cut(s)	674, 1319
PflMI	CCANNNNNTGG	1 cut(s)	1595
PkrI	GCNGC	4 cut(s)	262, 439, 646, 1180
PpuMI	RGGWCCY	1 cut(s)	1447
PsiI	TTATAA	2 cut(s)	431, 1257
Psp124BI	GAGCTC	1 cut(s)	1305
Psp5II	RGGWCCY	1 cut(s)	1447
Psp6I	CCWGG	1 cut(s)	700
PspFI	CCCAGC	3 cut(s)	752, 791, 1439
PspGI	CCWGG	1 cut(s)	700
PspN4I	GGNNCC	6 cut(s)	273, 487, 987, 1448, 1449, 1528
PspPI	GGNCC	4 cut(s)	116, 271, 986, 1447
PspPPI	RGGWCCY	1 cut(s)	1447
PstI	CTGCAG	1 cut(s)	1266
PstNI	CAGNNNCTG	1 cut(s)	1439
PsuI	RGATCY	4 cut(s)	424, 799, 1347, 1357
PvuII	CAGCTG	2 cut(s)	263, 1181
RruI	TCGCGA	1 cut(s)	636
RsaI	GTAC	7 cut(s)	70, 577, 613, 725, 1089, 1372, 1504
RsaNI	GTAC	7 cut(s)	69, 576, 612, 724, 1088, 1371, 1503
RseI	CAYNNNNRTG	2 cut(s)	213, 1521
SacI	GAGCTC	1 cut(s)	1305
SaqAI	TTAA	1 cut(s)	84
SatI	GCNGC	4 cut(s)	261, 438, 645, 1179
Sau3AI	GATC	8 cut(s)	196, 313, 424, 775, 799, 1026, 1347, 1357
Sau96I	GGNCC	4 cut(s)	116, 271, 986, 1447
SbfI	CCTGCAGG	1 cut(s)	1266
ScrFI	CCNGG	1 cut(s)	702
SdaI	CCTGCAGG	1 cut(s)	1266
SduI	GDGCHC	4 cut(s)	759, 1305, 1345, 1518
SfaNI	GCATC	6 cut(s)	226, 449, 589, 674, 952, 1587
SfcI	CTRYAG	1 cut(s)	1262
Sfr274I	CTCGAG	1 cut(s)	1323
SinI	GGWCC	1 cut(s)	1447
SlaI	CTCGAG	1 cut(s)	1323
SmiMI	CAYNNNNRTG	2 cut(s)	213, 1521
SmlI	CTYRAG	1 cut(s)	1323
SmoI	CTYRAG	1 cut(s)	1323
Sse8387I	CCTGCAGG	1 cut(s)	1266
Sse9I	AATT	7 cut(s)	387, 492, 822, 869, 894, 1170, 1227
SsiI	CCGC	4 cut(s)	285, 350, 952, 1410
SspMI	CTAG	3 cut(s)	797, 849, 1361
SstI	GAGCTC	1 cut(s)	1305
StyD4I	CCNGG	1 cut(s)	700
StyI	CCWWGG	1 cut(s)	444
TaaI	ACNGT	6 cut(s)	589, 652, 839, 1004, 1285, 1427
TaiI	ACGT	3 cut(s)	179, 1504, 1521
TaqI	TCGA	2 cut(s)	1029, 1324
TasI	AATT	7 cut(s)	387, 492, 822, 869, 894, 1170, 1227
TatI	WGTACW	4 cut(s)	68, 575, 723, 1087
TfiI	GAWTC	2 cut(s)	674, 1319
Tru1I	TTAA	1 cut(s)	84
Tru9I	TTAA	1 cut(s)	84
TscAI	CASTG	5 cut(s)	592, 600, 1111, 1288, 1564
TseFI	GTSAC	2 cut(s)	550, 1277
TseI	GCWGC	4 cut(s)	260, 437, 644, 1178
Tsp45I	GTSAC	2 cut(s)	550, 1277
TspDTI	ATGAA	7 cut(s)	321, 417, 524, 576, 1178, 1230, 1391
TspGWI	ACGGA	3 cut(s)	498, 531, 982
TspRI	CASTG	5 cut(s)	592, 600, 1111, 1288, 1564
Van91I	CCANNNNNTGG	1 cut(s)	1595
VneI	GTGCAC	1 cut(s)	1341
VpaK11BI	GGWCC	1 cut(s)	1447
XagI	CCTNNNNNAGG	1 cut(s)	330
XhoI	CTCGAG	1 cut(s)	1323
XmnI	GAANNNNTTC	1 cut(s)	366
XspI	CTAG	3 cut(s)	797, 849, 1361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.