Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0012821

Physical Location & Seq

Genomic Coordinates Forward (+)

68214632 .. 68220690

Gene Structure 6059 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00131530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGGCTTCTATCAACCTCTATTGCTCCCCAATGGCTCTCTTCCCTTCTCAAAGGAACAATTGTTTATATCCCGGGACTTCGGTTAAGCCAAATGGGTGCGTATCTGATTTTGTTGCACCGAGTTTGACTCGCAAGTTGTGCTTTGGTTTGAGGGGTGATTTCCAGGCAAGGGCTATGGTGATATCTGGAGAAGACGATCTCCTGTCTCACACTAATGGGGTTGAAGCACAACCTGACGCATTGAGTTTTGGAACACTTGCAGCTGATATGGCTTCTCTTAGTAGCGGTTTCTCTTCTGATAACGATGAATACGATCTAGATTATCCTATTGAAGGATTTTCTTCCATTGCGGAGGCCATTGAAGATATTCGTCAAGGCAAGATGGTAATTGTTGTAGATGATGAAGATAGAGAAAACGAGGGAGATCTTATAATGGCAGCATCCTTGGCAACACCAGAAGCTATGGCTTTTATTGTCAAGCACGGAACCGGAATTGTTTGTGTGAGCATGAAAGGAGAAGACTTAGAAAGGTTACAACTTCCGTTGATGGTGACACAGAATGAAGAAAAACTTTGTACAGCATTCACTGTCTCAGTGGATGCAAAACATGGTACGACAACTGGTGTTTCAGCTCGCGATAGAGCTGCAACAGTCTTGGCCCTTGCATCAAGGGATTCAAAACCTGAAGACTTCAACCGACCAGGTCATATCTTTCCCCTAAAGTACAGGGAGGGAGGAGTTCTAAAAAGAGCTGGGCACACAGAAGCTTCAGTTGATCTCGCAATGTTGGCTGGGCTAGATCCGATAGCAGTTTTGTGTGAAATTGTGGATGATGACGGTTCTATGGCTAGATTACCGAGGCTTCGTGAATTTGCAAAGGCAGAAAACTTGAAAATTATTTCTATTGCTGATTTGATAAGATATAGGAGAAAAAGGGACAAGTTGGTGGAGCGGGCTGGAGCTGCACGGATACCCACAATGTGGGGGCCATTTGAAGCCTACTGTTATAGGTCATTGCTAGATGGGATTGAGCACATTGCTATGGTTAAAGGTGATATTGGAGATGGGCAAAATATACTTGTGAGAGTACATTCAGAGTGTCTCACCGGTGACATCTTTGGGTCGGCCAGATGTGATTGTGGAAACCAGCTGGCACTTGCTATGAAACAAATTGAAGCAGCTGGTAGGGGTGTTTTAGTGTATCTCCGTGGACATGAAGGTAGAGGAATTGGTTTAGGCCACAAGCTCCGAGCTTATAACCTGCAGGATGATGGGCGTGACACTGTTGAAGCCAATGAGGAGCTCGGACTACCTGTTGATTCTCGCGAGTATGGAATAGGTGCACAGATACTACGAGATCTAGGTGTTCGTACAATGAAGCTGATGACAAACAATCCTGCAAAGTATAGCGGGCTCAAAGGTTATGGTTTGGCCGTTGCTGGCAGGGTCCCGTTATTGACACCAATAACTATGGAGAATAAGAGATATTTGGAGACTAAACGAACAAAAATGGGGCATGTCTATGGCTCCAATCTCAATGGTCATGTAGATGGCACCAGTGACGAAAGCAGTTCAAGCATTGGCAACCCATCTAATGGTGTCTCTGAGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006766 GO:0006767 GO:0006771 GO:0006807 GO:0008150 GO:0008152 GO:0008686 GO:0009058 GO:0009110 GO:0009231 GO:0009507 GO:0009536 GO:0009987 GO:0016829 GO:0016830 GO:0017144 GO:0018130 GO:0034641 GO:0042364 GO:0042726 GO:0042727 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00740 ko00790 ko01100 ko01110 map00740 map00790 map01100 map01110

Pfam Domains

Protein Families

DHBP_synthase GTP_cyclohydro2

Protein Analysis

537

Amino Acids

58.12

Weight (kDa)

5.39

Isoelectric Point (pI)

31.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHBP_synthase	PF00926	118 - 308	8.1e-88	3,4-dihydroxy-2-butanone 4-phosphate synthase
GTP_cyclohydro2	PF00925	319 - 484	1.5e-71	GTP cyclohydrolase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64300
fragaria_vesca	FvH4_1g20220 FvH4_1g20220 FvH4_1g20220 FvH4_1g20220
malus_domestica	MD01G1036500.v1.1
prunus_persica	Prupe.6G193700_v2.0.a1 Prupe.6G193700_v2.0.a1
pyrus_communis	pycom01g06320 pycom15g28070
rosa_chinensis	RchiOBHm_Chr2g0112291
rosa_laevigata	RLG00000017969
rosa_roxburghii	Rroxscaffold_2G00131530
rosa_samantha	Rh2AG232400 Rh2BG245800 Rh2CG236600 Rh2DG240100
rosa_wichuraiana	Rw2G018030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	431, 1257
Acc36I	ACCTGC	1 cut(s)	1269
AccB1I	GGYRCC	1 cut(s)	1553
AccB7I	CCANNNNNTGG	2 cut(s)	981, 1595
AccBSI	CCGCTC	1 cut(s)	952
AccII	CGCG	2 cut(s)	636, 1326
AciI	CCGC	4 cut(s)	285, 350, 952, 1410
AclWI	GGATC	1 cut(s)	794
AcoI	YGGCCR	2 cut(s)	1125, 1431
AcsI	RAATTY	1 cut(s)	869
AcuI	CTGAAG	2 cut(s)	705, 753
AdeI	CACNNNGTG	1 cut(s)	981
AfaI	GTAC	5 cut(s)	577, 613, 725, 1089, 1372
AfiI	CCNNNNNNNGG	4 cut(s)	169, 332, 981, 1595
AgeI	ACCGGT	1 cut(s)	1106
AjnI	CCWGG	2 cut(s)	162, 700
Alw21I	GWGCWC	3 cut(s)	1035, 1305, 1345
Alw26I	GTCTC	6 cut(s)	210, 595, 1106, 1487, 1601, 1606
Alw44I	GTGCAC	1 cut(s)	1341
AlwI	GGATC	1 cut(s)	794
Ama87I	CYCGRG	1 cut(s)	71
AoxI	GGCC	6 cut(s)	354, 657, 986, 1125, 1237, 1431
ApaLI	GTGCAC	1 cut(s)	1341
ApeKI	GCWGC	5 cut(s)	260, 437, 644, 962, 1178
ApoI	RAATTY	1 cut(s)	869
AsiGI	ACCGGT	1 cut(s)	1106
Asp700I	GAANNNNTTC	1 cut(s)	366
AspS9I	GGNCC	3 cut(s)	658, 986, 1447
AsuC2I	CCSGG	2 cut(s)	72, 73
AsuHPI	GGTGA	6 cut(s)	167, 190, 562, 1064, 1096, 1121
AvaI	CYCGRG	1 cut(s)	71
AvaII	GGWCC	1 cut(s)	1447
BaeGI	GKGCMC	2 cut(s)	759, 1345
BanI	GGYRCC	1 cut(s)	1553
BanII	GRGCYC	2 cut(s)	1305, 1416
BbsI	GAAGAC	3 cut(s)	198, 525, 693
Bbv12I	GWGCWC	3 cut(s)	1035, 1305, 1345
BbvI	GCAGC	5 cut(s)	272, 449, 631, 949, 1190
BccI	CCATC	7 cut(s)	376, 541, 1016, 1058, 1265, 1544, 1597
BceAI	ACGGC	1 cut(s)	1418
BcgI	CGANNNNNNTGC	2 cut(s)	626, 660
BciT130I	CCWGG	2 cut(s)	164, 702
BciVI	GTATCC	1 cut(s)	963
BcnI	CCSGG	2 cut(s)	72, 73
BcoDI	GTCTC	6 cut(s)	210, 595, 1106, 1487, 1601, 1606
BfaI	CTAG	5 cut(s)	317, 797, 849, 1019, 1361
BfmI	CTRYAG	1 cut(s)	1262
BfuAI	ACCTGC	1 cut(s)	1269
BfuI	GTATCC	1 cut(s)	963
BglII	AGATCT	2 cut(s)	424, 1357
BisI	GCNGC	5 cut(s)	261, 438, 645, 963, 1179
BlsI	GCNGC	5 cut(s)	262, 439, 646, 964, 1180
Bme1390I	CCNGG	4 cut(s)	72, 73, 164, 702
Bme18I	GGWCC	1 cut(s)	1447
BmeT110I	CYCGRG	1 cut(s)	71
BmgT120I	GGNCC	3 cut(s)	658, 986, 1447
BmiI	GGNNCC	6 cut(s)	487, 987, 1448, 1449, 1528, 1555
BmrFI	CCNGG	4 cut(s)	72, 73, 164, 702
BmsI	GCATC	3 cut(s)	449, 589, 674
BpiI	GAAGAC	3 cut(s)	198, 525, 693
BplI	GAGNNNNNCTC	2 cut(s)	112, 144
BpmI	CTGGAG	2 cut(s)	207, 978
BpuMI	CCSGG	2 cut(s)	72, 73
BsaJI	CCNNGG	4 cut(s)	71, 444, 857, 1207
BsaWI	WCCGGW	2 cut(s)	488, 1106
Bsc4I	CCNNNNNNNGG	4 cut(s)	169, 332, 981, 1595
Bse118I	RCCGGY	1 cut(s)	1106
Bse1I	ACTGG	2 cut(s)	625, 1557
Bse3DI	GCAATG	4 cut(s)	345, 789, 1013, 1035
BseBI	CCWGG	2 cut(s)	164, 702
BseDI	CCNNGG	4 cut(s)	71, 444, 857, 1207
BseGI	GGATG	4 cut(s)	440, 604, 835, 1273
BseLI	CCNNNNNNNGG	4 cut(s)	169, 332, 981, 1595
BseMI	GCAATG	4 cut(s)	345, 789, 1013, 1035
BseMII	CTCAG	2 cut(s)	606, 1596
BseNI	ACTGG	2 cut(s)	625, 1557
BseRI	GAGGAG	2 cut(s)	750, 1313
BseSI	GKGCMC	2 cut(s)	759, 1345
BseXI	GCAGC	5 cut(s)	272, 449, 631, 949, 1190
BseYI	CCCAGC	2 cut(s)	752, 791
BsgI	GTGCAG	1 cut(s)	948
Bsh1236I	CGCG	2 cut(s)	636, 1326
BshFI	GGCC	6 cut(s)	356, 659, 988, 1127, 1239, 1433
BshNI	GGYRCC	1 cut(s)	1553
BshTI	ACCGGT	1 cut(s)	1106
BsiHKAI	GWGCWC	3 cut(s)	1035, 1305, 1345
BsiHKCI	CYCGRG	1 cut(s)	71
BsiSI	CCGG	3 cut(s)	72, 489, 1107
BslFI	GGGAC	3 cut(s)	88, 950, 1433
BslI	CCNNNNNNNGG	4 cut(s)	169, 332, 981, 1595
BsmAI	GTCTC	6 cut(s)	210, 595, 1106, 1487, 1601, 1606
BsmFI	GGGAC	3 cut(s)	88, 950, 1433
BsmI	GAATGC	1 cut(s)	581
BsnI	GGCC	6 cut(s)	356, 659, 988, 1127, 1239, 1433
BsoBI	CYCGRG	1 cut(s)	71
Bsp1286I	GDGCHC	5 cut(s)	759, 1035, 1305, 1345, 1416
Bsp1407I	TGTACA	1 cut(s)	575
Bsp143I	GATC	6 cut(s)	196, 313, 424, 775, 799, 1357
Bsp68I	TCGCGA	2 cut(s)	636, 1326
BspACI	CCGC	4 cut(s)	285, 350, 952, 1410
BspANI	GGCC	6 cut(s)	356, 659, 988, 1127, 1239, 1433
BspCNI	CTCAG	2 cut(s)	605, 1597
BspFNI	CGCG	2 cut(s)	636, 1326
BspLI	GGNNCC	6 cut(s)	487, 987, 1448, 1449, 1528, 1555
BspMAI	CTGCAG	1 cut(s)	1266
BspMI	ACCTGC	1 cut(s)	1269
BspPI	GGATC	1 cut(s)	794
BspT107I	GGYRCC	1 cut(s)	1553
BsrBI	CCGCTC	1 cut(s)	952
BsrDI	GCAATG	4 cut(s)	345, 789, 1013, 1035
BsrFI	RCCGGY	1 cut(s)	1106
BsrGI	TGTACA	1 cut(s)	575
BsrI	ACTGG	2 cut(s)	625, 1557
BssAI	RCCGGY	1 cut(s)	1106
BssECI	CCNNGG	4 cut(s)	71, 444, 857, 1207
BssMI	GATC	6 cut(s)	196, 313, 424, 775, 799, 1357
BssT1I	CCWWGG	1 cut(s)	444
Bst2UI	CCWGG	2 cut(s)	164, 702
Bst4CI	ACNGT	5 cut(s)	589, 652, 839, 1004, 1285
Bst6I	CTCTTC	2 cut(s)	44, 298
BstAPI	GCANNNNNTGC	1 cut(s)	138
BstAUI	TGTACA	1 cut(s)	575
BstC8I	GCNNGC	5 cut(s)	634, 954, 1152, 1412, 1441
BstDEI	CTNAG	4 cut(s)	278, 523, 592, 1605
BstDSI	CCRYGG	1 cut(s)	1207
BstENI	CCTNNNNNAGG	1 cut(s)	330
BstF5I	GGATG	4 cut(s)	440, 604, 835, 1273
BstFNI	CGCG	2 cut(s)	636, 1326
BstKTI	GATC	6 cut(s)	199, 316, 427, 778, 802, 1360
BstMAI	GTCTC	6 cut(s)	210, 595, 1106, 1487, 1601, 1606
BstMBI	GATC	6 cut(s)	196, 313, 424, 775, 799, 1357
BstMWI	GCNNNNNNNGC	6 cut(s)	138, 269, 446, 788, 962, 1575
BstNI	CCWGG	2 cut(s)	164, 702
BstNSI	RCATGY	1 cut(s)	1520
BstSCI	CCNGG	4 cut(s)	70, 71, 162, 700
BstSFI	CTRYAG	1 cut(s)	1262
BstSLI	GKGCMC	2 cut(s)	759, 1345
BstUI	CGCG	2 cut(s)	636, 1326
BstV1I	GCAGC	5 cut(s)	272, 449, 631, 949, 1190
BstV2I	GAAGAC	3 cut(s)	198, 525, 693
BstX2I	RGATCY	3 cut(s)	424, 799, 1357
BstYI	RGATCY	3 cut(s)	424, 799, 1357
BsuI	GTATCC	1 cut(s)	963
BsuRI	GGCC	6 cut(s)	356, 659, 988, 1127, 1239, 1433
BtgI	CCRYGG	1 cut(s)	1207
BtsCI	GGATG	4 cut(s)	440, 604, 835, 1273
BtsIMutI	CAGTG	4 cut(s)	585, 600, 1281, 1564
BtuMI	TCGCGA	2 cut(s)	636, 1326
BveI	ACCTGC	1 cut(s)	1269
Cac8I	GCNNGC	5 cut(s)	634, 954, 1152, 1412, 1441
Cfr10I	RCCGGY	1 cut(s)	1106
Cfr13I	GGNCC	3 cut(s)	658, 986, 1447
Cfr9I	CCCGGG	1 cut(s)	71
CseI	GACGC	1 cut(s)	245
CsiI	ACCWGGT	1 cut(s)	700
Csp6I	GTAC	5 cut(s)	576, 612, 724, 1088, 1371
CspAI	ACCGGT	1 cut(s)	1106
CviAII	CATG	5 cut(s)	508, 608, 1214, 1517, 1544
CviQI	GTAC	5 cut(s)	576, 612, 724, 1088, 1371
DdeI	CTNAG	4 cut(s)	278, 523, 592, 1605
DpnI	GATC	6 cut(s)	198, 315, 426, 777, 801, 1359
DpnII	GATC	6 cut(s)	196, 313, 424, 775, 799, 1357
DraIII	CACNNNGTG	1 cut(s)	981
EaeI	YGGCCR	2 cut(s)	1125, 1431
Eam1104I	CTCTTC	2 cut(s)	44, 298
EarI	CTCTTC	2 cut(s)	44, 298
Ecl136II	GAGCTC	1 cut(s)	1303
Eco130I	CCWWGG	1 cut(s)	444
Eco24I	GRGCYC	2 cut(s)	1305, 1416
Eco32I	GATATC	1 cut(s)	183
Eco47I	GGWCC	1 cut(s)	1447
Eco53kI	GAGCTC	1 cut(s)	1303
Eco57I	CTGAAG	2 cut(s)	705, 753
Eco88I	CYCGRG	1 cut(s)	71
EcoICRI	GAGCTC	1 cut(s)	1303
EcoNI	CCTNNNNNAGG	1 cut(s)	330
EcoO109I	RGGNCCY	1 cut(s)	1447
EcoRII	CCWGG	2 cut(s)	162, 700
EcoRV	GATATC	1 cut(s)	183
EcoT14I	CCWWGG	1 cut(s)	444
EcoT38I	GRGCYC	2 cut(s)	1305, 1416
ErhI	CCWWGG	1 cut(s)	444
FaeI	CATG	5 cut(s)	511, 611, 1217, 1520, 1547
FalI	AAGNNNNNCTT	4 cut(s)	125, 157, 555, 587
FaqI	GGGAC	3 cut(s)	88, 950, 1433
FatI	CATG	5 cut(s)	507, 607, 1213, 1516, 1543
FauI	CCCGC	2 cut(s)	945, 1403
Fnu4HI	GCNGC	5 cut(s)	261, 438, 645, 963, 1179
FokI	GGATG	4 cut(s)	427, 611, 842, 1280
FriOI	GRGCYC	2 cut(s)	1305, 1416
Fsp4HI	GCNGC	5 cut(s)	261, 438, 645, 963, 1179
FspBI	CTAG	5 cut(s)	317, 797, 849, 1019, 1361
GluI	GCNGC	5 cut(s)	261, 438, 645, 963, 1179
GsaI	CCCAGC	2 cut(s)	756, 795
GsuI	CTGGAG	2 cut(s)	207, 978
HaeIII	GGCC	6 cut(s)	356, 659, 988, 1127, 1239, 1433
HapII	CCGG	3 cut(s)	72, 489, 1107
HgaI	GACGC	1 cut(s)	245
Hin1II	CATG	5 cut(s)	511, 611, 1217, 1520, 1547
HindIII	AAGCTT	1 cut(s)	765
HinfI	GANTC	3 cut(s)	127, 674, 1319
HpaII	CCGG	3 cut(s)	72, 489, 1107
HphI	GGTGA	6 cut(s)	167, 190, 562, 1064, 1096, 1121
Hpy166II	GTNNAC	2 cut(s)	1211, 1343
Hpy188I	TCNGA	7 cut(s)	106, 298, 804, 1096, 1250, 1307, 1606
Hpy188III	TCNNGA	5 cut(s)	186, 317, 635, 866, 1325
Hpy8I	GTNNAC	2 cut(s)	1211, 1343
HpyAV	CCTTC	3 cut(s)	54, 326, 1211
HpyCH4III	ACNGT	5 cut(s)	589, 652, 839, 1004, 1285
HpyF10VI	GCNNNNNNNGC	6 cut(s)	138, 269, 446, 788, 962, 1575
HpyF3I	CTNAG	4 cut(s)	278, 523, 592, 1605
Hsp92II	CATG	5 cut(s)	511, 611, 1217, 1520, 1547
KflI	GGGWCCC	1 cut(s)	1447
Kzo9I	GATC	6 cut(s)	196, 313, 424, 775, 799, 1357
LmnI	GCTCC	6 cut(s)	29, 949, 959, 1251, 1300, 1532
Lsp1109I	GCAGC	5 cut(s)	272, 449, 631, 949, 1190
LweI	GCATC	3 cut(s)	449, 589, 674
MabI	ACCWGGT	1 cut(s)	700
MaeI	CTAG	5 cut(s)	317, 797, 849, 1019, 1361
MaeIII	GTNAC	5 cut(s)	531, 550, 1109, 1277, 1559
MalI	GATC	6 cut(s)	198, 315, 426, 777, 801, 1359
MbiI	CCGCTC	1 cut(s)	952
MboI	GATC	6 cut(s)	196, 313, 424, 775, 799, 1357
MboII	GAAGA	9 cut(s)	31, 203, 285, 333, 374, 416, 530, 575, 698
MfeI	CAATTG	1 cut(s)	58
MflI	RGATCY	3 cut(s)	424, 799, 1357
MhlI	GDGCHC	5 cut(s)	759, 1035, 1305, 1345, 1416
MluCI	AATT	8 cut(s)	58, 387, 492, 822, 869, 894, 1170, 1227
MlyI	GAGTC	1 cut(s)	121
MnlI	CCTC	9 cut(s)	26, 144, 346, 412, 724, 728, 852, 1217, 1291
MroXI	GAANNNNTTC	1 cut(s)	366
MseI	TTAA	2 cut(s)	84, 1047
MslI	CAYNNNNRTG	4 cut(s)	213, 1040, 1521, 1548
MspA1I	CMGCKG	3 cut(s)	263, 1150, 1181
MspI	CCGG	3 cut(s)	72, 489, 1107
MspR9I	CCNGG	4 cut(s)	72, 73, 164, 702
MunI	CAATTG	1 cut(s)	58
Mva1269I	GAATGC	1 cut(s)	581
MvaI	CCWGG	2 cut(s)	164, 702
MvnI	CGCG	2 cut(s)	636, 1326
MwoI	GCNNNNNNNGC	6 cut(s)	138, 269, 446, 788, 962, 1575
NciI	CCSGG	2 cut(s)	72, 73
NdeII	GATC	6 cut(s)	196, 313, 424, 775, 799, 1357
NlaIII	CATG	5 cut(s)	511, 611, 1217, 1520, 1547
NlaIV	GGNNCC	6 cut(s)	487, 987, 1448, 1449, 1528, 1555
NmuCI	GTSAC	4 cut(s)	550, 1109, 1277, 1559
NruI	TCGCGA	2 cut(s)	636, 1326
NspI	RCATGY	1 cut(s)	1520
PcsI	WCGNNNNNNNCGW	1 cut(s)	309
PctI	GAATGC	1 cut(s)	581
PdmI	GAANNNNTTC	1 cut(s)	366
PfeI	GAWTC	2 cut(s)	674, 1319
PflFI	GACNNNGTC	1 cut(s)	702
PflMI	CCANNNNNTGG	2 cut(s)	981, 1595
PinAI	ACCGGT	1 cut(s)	1106
PkrI	GCNGC	5 cut(s)	262, 439, 646, 964, 1180
PleI	GAGTC	1 cut(s)	121
PpsI	GAGTC	1 cut(s)	121
PpuMI	RGGWCCY	1 cut(s)	1447
PsiI	TTATAA	2 cut(s)	431, 1257
Psp124BI	GAGCTC	1 cut(s)	1305
Psp5II	RGGWCCY	1 cut(s)	1447
Psp6I	CCWGG	2 cut(s)	162, 700
PspFI	CCCAGC	2 cut(s)	752, 791
PspGI	CCWGG	2 cut(s)	162, 700
PspN4I	GGNNCC	6 cut(s)	487, 987, 1448, 1449, 1528, 1555
PspPI	GGNCC	3 cut(s)	658, 986, 1447
PspPPI	RGGWCCY	1 cut(s)	1447
PstI	CTGCAG	1 cut(s)	1266
PsuI	RGATCY	3 cut(s)	424, 799, 1357
PsyI	GACNNNGTC	1 cut(s)	702
PvuII	CAGCTG	3 cut(s)	263, 1150, 1181
RruI	TCGCGA	2 cut(s)	636, 1326
RsaI	GTAC	5 cut(s)	577, 613, 725, 1089, 1372
RsaNI	GTAC	5 cut(s)	576, 612, 724, 1088, 1371
RseI	CAYNNNNRTG	4 cut(s)	213, 1040, 1521, 1548
SacI	GAGCTC	1 cut(s)	1305
SaqAI	TTAA	2 cut(s)	84, 1047
SatI	GCNGC	5 cut(s)	261, 438, 645, 963, 1179
Sau3AI	GATC	6 cut(s)	196, 313, 424, 775, 799, 1357
Sau96I	GGNCC	3 cut(s)	658, 986, 1447
SbfI	CCTGCAGG	1 cut(s)	1266
SchI	GAGTC	1 cut(s)	121
ScrFI	CCNGG	4 cut(s)	72, 73, 164, 702
SdaI	CCTGCAGG	1 cut(s)	1266
SduI	GDGCHC	5 cut(s)	759, 1035, 1305, 1345, 1416
SexAI	ACCWGGT	1 cut(s)	700
SfaNI	GCATC	3 cut(s)	449, 589, 674
SfcI	CTRYAG	1 cut(s)	1262
SgrAI	CRCCGGYG	1 cut(s)	1106
SinI	GGWCC	1 cut(s)	1447
SmaI	CCCGGG	1 cut(s)	73
SmiMI	CAYNNNNRTG	4 cut(s)	213, 1040, 1521, 1548
Sse8387I	CCTGCAGG	1 cut(s)	1266
Sse9I	AATT	8 cut(s)	58, 387, 492, 822, 869, 894, 1170, 1227
SsiI	CCGC	4 cut(s)	285, 350, 952, 1410
SspMI	CTAG	5 cut(s)	317, 797, 849, 1019, 1361
SstI	GAGCTC	1 cut(s)	1305
StyD4I	CCNGG	4 cut(s)	70, 71, 162, 700
StyI	CCWWGG	1 cut(s)	444
TaaI	ACNGT	5 cut(s)	589, 652, 839, 1004, 1285
TasI	AATT	8 cut(s)	58, 387, 492, 822, 869, 894, 1170, 1227
TatI	WGTACW	3 cut(s)	575, 723, 1087
TfiI	GAWTC	2 cut(s)	674, 1319
Tru1I	TTAA	2 cut(s)	84, 1047
Tru9I	TTAA	2 cut(s)	84, 1047
TscAI	CASTG	4 cut(s)	592, 600, 1288, 1564
TseFI	GTSAC	4 cut(s)	550, 1109, 1277, 1559
TseI	GCWGC	5 cut(s)	260, 437, 644, 962, 1178
Tsp45I	GTSAC	4 cut(s)	550, 1109, 1277, 1559
TspDTI	ATGAA	7 cut(s)	321, 417, 524, 576, 1178, 1230, 1391
TspGWI	ACGGA	4 cut(s)	498, 531, 982, 1196
TspMI	CCCGGG	1 cut(s)	71
TspRI	CASTG	4 cut(s)	592, 600, 1288, 1564
Tth111I	GACNNNGTC	1 cut(s)	702
Van91I	CCANNNNNTGG	2 cut(s)	981, 1595
VneI	GTGCAC	1 cut(s)	1341
VpaK11BI	GGWCC	1 cut(s)	1447
XagI	CCTNNNNNAGG	1 cut(s)	330
XapI	RAATTY	1 cut(s)	869
XbaI	TCTAGA	1 cut(s)	316
XceI	RCATGY	1 cut(s)	1520
XmaI	CCCGGG	1 cut(s)	71
XmnI	GAANNNNTTC	1 cut(s)	366
XspI	CTAG	5 cut(s)	317, 797, 849, 1019, 1361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.