Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012821

Physical Location & Seq

Genomic Coordinates Forward (+)

12251507 .. 12257759

Gene Structure 6253 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1036500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGGCTTCCATCAACCCCTATTGCTCCTCAACGGCTCTTTCCCCTTCTCCAGGGCACACTATTCTATATCCTGGGACTTCGCTTGCAGCAAATGGGCGATTATCCGGTTTTGTCACACCGAGTGTATCATGGAAATTGTTCTTTCATTCCAAGGGATCAATTAAGGCAAGAGCTTTGCTGGGAGAAGATGGTCTCCTGTCTTACCCAAATGGCAACCCAACTGGGAGTGAAATACAACCTGATGCATTAGGTTTCGGAACAGTTTCAGCAGAAACAACCCCAACGTTTAGTGGGTTCTCTTCTGAAAATGATGAAGGTGATCTAGATCACCCTATACATGGATTTTCTTCCATTCCAGAGGCCATTGAAGATATACGTCAAGGCAAGATGGTTATCGTTGTAGATGATGAAGATAGAGAAAATGAGGGAGATCTTATAATGGCGGCTTCCTTGGCAACTCCAGAAGCTATGGCATTTATTGTGAAGCACGGAACTGGGATTGTTTGTGTGAGCATGAAAGGAGAAGACTTGGAGAGGTTGCAACTTCCACTTATGGTGACACAGAATGAAGAAAAACTTTGTACGGCATTCACTGTCTCAGTGGATGCAAAACGTGGTACGACAACTGGTGTTTCTGCTCGTGATAGAGCTGCAACAGTCTTGGCTCTTGCATCAAGAGATTCAACACCTGAAGATTTCAACCGCCCAGGACATATCTTTCCCCTAAAGTACAGAGAGGGAGGAGTTCTAAAAAGAGCCGGTCACACTGAAGCTTCAGTTGATCTTGCTATGTTGGCTGGGCTGGATCCAGTAGCAGTTTTATGTGAAATTGTGGATGATGACGGTTCTATGGCTAGGTTACCAAAGCTTCTTGAATTTGCAAGGGCAGAAAACTTGAAAATTGTTTCCATTGCTGATTTGATAAGATATAGAAGGAAAAGAGATAAGTTGGTGGAGAGGGCTGGAGTTGCACGCATACCCACAATGTGGGGGCCATTTGAAGCCTACTGTTATAAATCATTGCTCGATGGGATTGAGCACATTGCTATGGTTAAAGGTGAAATTGCTGACGGGCAAGAAATACTTGTGAGGGTACACTCTGAGTGCCTCACTGGTGACATATTTGGGTCAGCCAGATGTGACTGTGGAAACCAGCTTGCACTTGCAATGAAGCAAATTGAAGCAGCTGGTAGGGGTGTTTTGGTGTATCTCCGTGGACATGAAGGTAGAGGAATTGGTTTGGGTCACAAGCTCCGAGCTTATACACTGCAGGATGCTGGGCGTGACACTGTTGAAGCCAATGAGGAGCTGGGATTACCTGTTGATTCTCGCGAGTATGGCATAGGTGCACAGATACTACGAGATCTAGGTGTTCGAACAATGAAGCTGATGACGAACAATCCTGCAAAATATATTGGTCTCAAGGGTTATGGTTTGGCCATTGCTGGCAGAGTCCCATTGTTGGCACCAATAACTATGGAGAACCAGAAATACTTGGAGACTAAACGGGCAAAAATGGGGCACATCTACGGTTCAAAAAGTAATGGTCATGTAAACGGCACAATCGATGAAAGTAGTTCGAAAATTGACAACGAATCTAATGGTGTCCCCGGGACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006766 GO:0006767 GO:0006771 GO:0006807 GO:0008150 GO:0008152 GO:0008686 GO:0009058 GO:0009110 GO:0009231 GO:0009507 GO:0009536 GO:0009987 GO:0016829 GO:0016830 GO:0017144 GO:0018130 GO:0034641 GO:0042364 GO:0042726 GO:0042727 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00740 ko00790 ko01100 ko01110 map00740 map00790 map01100 map01110

Pfam Domains

Protein Families

DHBP_synthase GTP_cyclohydro2

Protein Analysis

540

Amino Acids

57.94

Weight (kDa)

5.71

Isoelectric Point (pI)

31.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHBP_synthase	PF00926	120 - 310	1.4e-87	3,4-dihydroxy-2-butanone 4-phosphate synthase
GTP_cyclohydro2	PF00925	323 - 486	1.6e-70	GTP cyclohydrolase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64300
fragaria_vesca	FvH4_1g20220 FvH4_1g20220 FvH4_1g20220 FvH4_1g20220
malus_domestica	MD01G1036500.v1.1
prunus_persica	Prupe.6G193700_v2.0.a1 Prupe.6G193700_v2.0.a1
pyrus_communis	pycom01g06320 pycom15g28070
rosa_chinensis	RchiOBHm_Chr2g0112291
rosa_laevigata	RLG00000017969
rosa_roxburghii	Rroxscaffold_2G00131530
rosa_samantha	Rh2AG232400 Rh2BG245800 Rh2CG236600 Rh2DG240100
rosa_wichuraiana	Rw2G018030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	437, 1014
AccB1I	GGYRCC	1 cut(s)	1465
AccB7I	CCANNNNNTGG	1 cut(s)	987
AccII	CGCG	1 cut(s)	1332
AciI	CCGC	2 cut(s)	443, 703
AclI	AACGTT	1 cut(s)	284
AclWI	GGATC	3 cut(s)	163, 800, 813
AcoI	YGGCCR	1 cut(s)	1437
AcsI	RAATTY	1 cut(s)	875
AcuI	CTGAAG	3 cut(s)	711, 759, 789
AdeI	CACNNNGTG	2 cut(s)	122, 987
AfaI	GTAC	4 cut(s)	583, 619, 731, 1095
AfiI	CCNNNNNNNGG	4 cut(s)	50, 338, 987, 1462
AgsI	TTSAA	9 cut(s)	368, 684, 700, 875, 898, 1001, 1181, 1295, 1536
AjiI	CACGTC	1 cut(s)	1617
AjnI	CCWGG	3 cut(s)	49, 70, 706
Alw21I	GWGCWC	2 cut(s)	1041, 1351
Alw26I	GTCTC	4 cut(s)	197, 601, 1424, 1493
Alw44I	GTGCAC	1 cut(s)	1347
AlwI	GGATC	3 cut(s)	163, 800, 813
Ama87I	CYCGRG	1 cut(s)	1610
AoxI	GGCC	3 cut(s)	360, 992, 1437
ApaLI	GTGCAC	1 cut(s)	1347
ApeKI	GCWGC	3 cut(s)	86, 650, 1184
ApoI	RAATTY	1 cut(s)	875
Asp700I	GAANNNNTTC	2 cut(s)	137, 262
AspS9I	GGNCC	1 cut(s)	992
AsuC2I	CCSGG	2 cut(s)	1611, 1612
AsuHPI	GGTGA	5 cut(s)	320, 329, 568, 1070, 1127
AsuII	TTCGAA	2 cut(s)	1375, 1580
AvaI	CYCGRG	1 cut(s)	1610
BaeGI	GKGCMC	3 cut(s)	57, 1351, 1524
BalI	TGGCCA	1 cut(s)	1439
BamHI	GGATCC	1 cut(s)	805
BanI	GGYRCC	1 cut(s)	1465
BauI	CACGAG	1 cut(s)	639
BbsI	GAAGAC	1 cut(s)	531
Bbv12I	GWGCWC	2 cut(s)	1041, 1351
BbvI	GCAGC	3 cut(s)	98, 637, 1196
BccI	CCATC	4 cut(s)	17, 182, 382, 1022
BceAI	ACGGC	3 cut(s)	48, 600, 1573
BciT130I	CCWGG	3 cut(s)	51, 72, 708
BcnI	CCSGG	2 cut(s)	1611, 1612
BcoDI	GTCTC	4 cut(s)	197, 601, 1424, 1493
BfaI	CTAG	3 cut(s)	323, 855, 1367
BfmI	CTRYAG	1 cut(s)	1268
BglII	AGATCT	2 cut(s)	430, 1363
BisI	GCNGC	4 cut(s)	87, 444, 651, 1185
BlsI	GCNGC	4 cut(s)	88, 445, 652, 1186
Bme1390I	CCNGG	5 cut(s)	51, 72, 708, 1611, 1612
BmeT110I	CYCGRG	1 cut(s)	1610
BmgBI	CACGTC	1 cut(s)	1617
BmgT120I	GGNCC	1 cut(s)	992
BmiI	GGNNCC	3 cut(s)	807, 993, 1467
BmrFI	CCNGG	5 cut(s)	51, 72, 708, 1611, 1612
BmrI	ACTGGG	2 cut(s)	231, 504
BmsI	GCATC	4 cut(s)	232, 595, 680, 1264
BmuI	ACTGGG	2 cut(s)	231, 504
BpiI	GAAGAC	1 cut(s)	531
BpmI	CTGGAG	3 cut(s)	33, 444, 984
Bpu14I	TTCGAA	2 cut(s)	1375, 1580
BpuEI	CTTGAG	1 cut(s)	1406
BpuMI	CCSGG	2 cut(s)	1611, 1612
Bsa29I	ATCGAT	1 cut(s)	1566
BsaBI	GATNNNNATC	2 cut(s)	324, 392
BsaI	GGTCTC	2 cut(s)	197, 1424
BsaJI	CCNNGG	8 cut(s)	50, 71, 150, 450, 706, 1213, 1609, 1610
BsaWI	WCCGGW	1 cut(s)	104
Bsc4I	CCNNNNNNNGG	4 cut(s)	50, 338, 987, 1462
Bse118I	RCCGGY	1 cut(s)	758
Bse1I	ACTGG	5 cut(s)	226, 499, 631, 809, 1117
Bse3DI	GCAATG	5 cut(s)	909, 1019, 1041, 1173, 1440
Bse8I	GATNNNNATC	2 cut(s)	324, 392
BseBI	CCWGG	3 cut(s)	51, 72, 708
BseCI	ATCGAT	1 cut(s)	1566
BseDI	CCNNGG	8 cut(s)	50, 71, 150, 450, 706, 1213, 1609, 1610
BseGI	GGATG	3 cut(s)	610, 841, 1279
BseJI	GATNNNNATC	2 cut(s)	324, 392
BseLI	CCNNNNNNNGG	4 cut(s)	50, 338, 987, 1462
BseMI	GCAATG	5 cut(s)	909, 1019, 1041, 1173, 1440
BseMII	CTCAG	2 cut(s)	612, 1092
BseNI	ACTGG	5 cut(s)	226, 499, 631, 809, 1117
BseRI	GAGGAG	3 cut(s)	16, 756, 1319
BseSI	GKGCMC	3 cut(s)	57, 1351, 1524
BseXI	GCAGC	3 cut(s)	98, 637, 1196
BseYI	CCCAGC	4 cut(s)	178, 797, 1277, 1309
Bsh1236I	CGCG	1 cut(s)	1332
BshFI	GGCC	3 cut(s)	362, 994, 1439
BshNI	GGYRCC	1 cut(s)	1465
BshVI	ATCGAT	1 cut(s)	1566
BsiHKAI	GWGCWC	2 cut(s)	1041, 1351
BsiHKCI	CYCGRG	1 cut(s)	1610
BsiSI	CCGG	3 cut(s)	105, 759, 1611
BslFI	GGGAC	3 cut(s)	88, 1439, 1592
BslI	CCNNNNNNNGG	4 cut(s)	50, 338, 987, 1462
BsmAI	GTCTC	4 cut(s)	197, 601, 1424, 1493
BsmFI	GGGAC	3 cut(s)	88, 1439, 1592
BsmI	GAATGC	1 cut(s)	587
BsnI	GGCC	3 cut(s)	362, 994, 1439
Bso31I	GGTCTC	2 cut(s)	197, 1424
BsoBI	CYCGRG	1 cut(s)	1610
Bsp119I	TTCGAA	2 cut(s)	1375, 1580
Bsp1286I	GDGCHC	4 cut(s)	57, 1041, 1351, 1524
Bsp143I	GATC	7 cut(s)	155, 319, 325, 430, 781, 805, 1363
Bsp68I	TCGCGA	1 cut(s)	1332
BspACI	CCGC	2 cut(s)	443, 703
BspANI	GGCC	3 cut(s)	362, 994, 1439
BspCNI	CTCAG	2 cut(s)	611, 1093
BspDI	ATCGAT	1 cut(s)	1566
BspFNI	CGCG	1 cut(s)	1332
BspLI	GGNNCC	3 cut(s)	807, 993, 1467
BspMAI	CTGCAG	1 cut(s)	1272
BspPI	GGATC	3 cut(s)	163, 800, 813
BspT104I	TTCGAA	2 cut(s)	1375, 1580
BspT107I	GGYRCC	1 cut(s)	1465
BspTNI	GGTCTC	2 cut(s)	197, 1424
BsrDI	GCAATG	5 cut(s)	909, 1019, 1041, 1173, 1440
BsrFI	RCCGGY	1 cut(s)	758
BsrI	ACTGG	5 cut(s)	226, 499, 631, 809, 1117
BssAI	RCCGGY	1 cut(s)	758
BssECI	CCNNGG	8 cut(s)	50, 71, 150, 450, 706, 1213, 1609, 1610
BssMI	GATC	7 cut(s)	155, 319, 325, 430, 781, 805, 1363
BssSI	CACGAG	1 cut(s)	639
BssT1I	CCWWGG	2 cut(s)	150, 450
Bst2BI	CACGAG	1 cut(s)	639
Bst2UI	CCWGG	3 cut(s)	51, 72, 708
Bst4CI	ACNGT	8 cut(s)	262, 595, 658, 845, 1010, 1145, 1291, 1532
Bst6I	CTCTTC	1 cut(s)	304
BstBI	TTCGAA	2 cut(s)	1375, 1580
BstC8I	GCNNGC	4 cut(s)	84, 973, 1158, 1447
BstDEI	CTNAG	2 cut(s)	598, 1101
BstDSI	CCRYGG	1 cut(s)	1213
BstEII	GGTNACC	1 cut(s)	858
BstENI	CCTNNNNNAGG	1 cut(s)	48
BstF5I	GGATG	3 cut(s)	610, 841, 1279
BstFNI	CGCG	1 cut(s)	1332
BstKTI	GATC	7 cut(s)	158, 322, 328, 433, 784, 808, 1366
BstMAI	GTCTC	4 cut(s)	197, 601, 1424, 1493
BstMBI	GATC	7 cut(s)	155, 319, 325, 430, 781, 805, 1363
BstMWI	GCNNNNNNNGC	4 cut(s)	452, 794, 968, 1338
BstNI	CCWGG	3 cut(s)	51, 72, 708
BstPI	GGTNACC	1 cut(s)	858
BstSCI	CCNGG	5 cut(s)	49, 70, 706, 1609, 1610
BstSFI	CTRYAG	1 cut(s)	1268
BstSLI	GKGCMC	3 cut(s)	57, 1351, 1524
BstUI	CGCG	1 cut(s)	1332
BstV1I	GCAGC	3 cut(s)	98, 637, 1196
BstV2I	GAAGAC	1 cut(s)	531
BstX2I	RGATCY	3 cut(s)	430, 805, 1363
BstYI	RGATCY	3 cut(s)	430, 805, 1363
Bsu15I	ATCGAT	1 cut(s)	1566
BsuRI	GGCC	3 cut(s)	362, 994, 1439
BsuTUI	ATCGAT	1 cut(s)	1566
BtgI	CCRYGG	1 cut(s)	1213
BtrI	CACGTC	1 cut(s)	1617
BtsCI	GGATG	3 cut(s)	610, 841, 1279
BtsI	GCAGTG	1 cut(s)	1265
BtsIMutI	CAGTG	6 cut(s)	591, 606, 765, 1110, 1265, 1287
BtuMI	TCGCGA	1 cut(s)	1332
Cac8I	GCNNGC	4 cut(s)	84, 973, 1158, 1447
Cfr10I	RCCGGY	1 cut(s)	758
Cfr13I	GGNCC	1 cut(s)	992
Cfr9I	CCCGGG	1 cut(s)	1610
ClaI	ATCGAT	1 cut(s)	1566
Csp6I	GTAC	4 cut(s)	582, 618, 730, 1094
CviAII	CATG	5 cut(s)	129, 338, 514, 1220, 1550
CviQI	GTAC	4 cut(s)	582, 618, 730, 1094
DdeI	CTNAG	2 cut(s)	598, 1101
DpnI	GATC	7 cut(s)	157, 321, 327, 432, 783, 807, 1365
DpnII	GATC	7 cut(s)	155, 319, 325, 430, 781, 805, 1363
DraIII	CACNNNGTG	2 cut(s)	122, 987
EaeI	YGGCCR	1 cut(s)	1437
Eam1104I	CTCTTC	1 cut(s)	304
EarI	CTCTTC	1 cut(s)	304
Eco130I	CCWWGG	2 cut(s)	150, 450
Eco31I	GGTCTC	2 cut(s)	197, 1424
Eco57I	CTGAAG	3 cut(s)	711, 759, 789
Eco88I	CYCGRG	1 cut(s)	1610
Eco91I	GGTNACC	1 cut(s)	858
EcoNI	CCTNNNNNAGG	1 cut(s)	48
EcoO65I	GGTNACC	1 cut(s)	858
EcoRII	CCWGG	3 cut(s)	49, 70, 706
EcoT14I	CCWWGG	2 cut(s)	150, 450
EcoT22I	ATGCAT	1 cut(s)	247
ErhI	CCWWGG	2 cut(s)	150, 450
FaeI	CATG	5 cut(s)	132, 341, 517, 1223, 1553
FalI	AAGNNNNNCTT	4 cut(s)	561, 593, 1068, 1100
FaqI	GGGAC	3 cut(s)	88, 1439, 1592
FatI	CATG	5 cut(s)	128, 337, 513, 1219, 1549
Fnu4HI	GCNGC	4 cut(s)	87, 444, 651, 1185
FokI	GGATG	3 cut(s)	617, 848, 1286
Fsp4HI	GCNGC	4 cut(s)	87, 444, 651, 1185
FspBI	CTAG	3 cut(s)	323, 855, 1367
GluI	GCNGC	4 cut(s)	87, 444, 651, 1185
GsaI	CCCAGC	4 cut(s)	182, 801, 1281, 1313
GsuI	CTGGAG	3 cut(s)	33, 444, 984
HaeIII	GGCC	3 cut(s)	362, 994, 1439
HapII	CCGG	3 cut(s)	105, 759, 1611
Hin1II	CATG	5 cut(s)	132, 341, 517, 1223, 1553
HindIII	AAGCTT	2 cut(s)	771, 866
HinfI	GANTC	4 cut(s)	680, 1325, 1452, 1595
HpaII	CCGG	3 cut(s)	105, 759, 1611
HphI	GGTGA	5 cut(s)	320, 329, 568, 1070, 1127
Hpy166II	GTNNAC	4 cut(s)	1096, 1217, 1349, 1555
Hpy188I	TCNGA	4 cut(s)	257, 304, 1102, 1256
Hpy188III	TCNNGA	7 cut(s)	323, 356, 461, 641, 675, 872, 1331
Hpy8I	GTNNAC	4 cut(s)	1096, 1217, 1349, 1555
HpyAV	CCTTC	4 cut(s)	54, 308, 927, 1217
HpyCH4III	ACNGT	8 cut(s)	262, 595, 658, 845, 1010, 1145, 1291, 1532
HpyCH4IV	ACGT	4 cut(s)	284, 376, 613, 1616
HpyF10VI	GCNNNNNNNGC	4 cut(s)	452, 794, 968, 1338
HpyF3I	CTNAG	2 cut(s)	598, 1101
HpySE526I	ACGT	4 cut(s)	284, 376, 613, 1616
Hsp92II	CATG	5 cut(s)	132, 341, 517, 1223, 1553
Kzo9I	GATC	7 cut(s)	155, 319, 325, 430, 781, 805, 1363
LmnI	GCTCC	3 cut(s)	29, 1257, 1306
Lsp1109I	GCAGC	3 cut(s)	98, 637, 1196
LweI	GCATC	4 cut(s)	232, 595, 680, 1264
MaeI	CTAG	3 cut(s)	323, 855, 1367
MaeII	ACGT	4 cut(s)	284, 376, 613, 1616
MaeIII	GTNAC	8 cut(s)	112, 556, 761, 858, 1115, 1139, 1244, 1283
MalI	GATC	7 cut(s)	157, 321, 327, 432, 783, 807, 1365
MboI	GATC	7 cut(s)	155, 319, 325, 430, 781, 805, 1363
MboII	GAAGA	8 cut(s)	197, 291, 339, 380, 422, 536, 581, 704
MflI	RGATCY	3 cut(s)	430, 805, 1363
MhlI	GDGCHC	4 cut(s)	57, 1041, 1351, 1524
MlsI	TGGCCA	1 cut(s)	1439
MluCI	AATT	9 cut(s)	134, 159, 828, 875, 900, 1062, 1176, 1233, 1584
MluNI	TGGCCA	1 cut(s)	1439
MlyI	GAGTC	1 cut(s)	1461
Mox20I	TGGCCA	1 cut(s)	1439
Mph1103I	ATGCAT	1 cut(s)	247
MroXI	GAANNNNTTC	2 cut(s)	137, 262
MscI	TGGCCA	1 cut(s)	1439
MseI	TTAA	2 cut(s)	162, 1053
MslI	CAYNNNNRTG	1 cut(s)	1046
Msp20I	TGGCCA	1 cut(s)	1439
MspA1I	CMGCKG	1 cut(s)	1187
MspI	CCGG	3 cut(s)	105, 759, 1611
MspR9I	CCNGG	5 cut(s)	51, 72, 708, 1611, 1612
Mva1269I	GAATGC	1 cut(s)	587
MvaI	CCWGG	3 cut(s)	51, 72, 708
MvnI	CGCG	1 cut(s)	1332
MwoI	GCNNNNNNNGC	4 cut(s)	452, 794, 968, 1338
NciI	CCSGG	2 cut(s)	1611, 1612
NdeII	GATC	7 cut(s)	155, 319, 325, 430, 781, 805, 1363
NlaIII	CATG	5 cut(s)	132, 341, 517, 1223, 1553
NlaIV	GGNNCC	3 cut(s)	807, 993, 1467
NmuCI	GTSAC	7 cut(s)	112, 556, 761, 1115, 1139, 1244, 1283
NruI	TCGCGA	1 cut(s)	1332
NsiI	ATGCAT	1 cut(s)	247
NspV	TTCGAA	2 cut(s)	1375, 1580
PcsI	WCGNNNNNNNCGW	1 cut(s)	1563
PctI	GAATGC	1 cut(s)	587
PdmI	GAANNNNTTC	2 cut(s)	137, 262
PfeI	GAWTC	3 cut(s)	680, 1325, 1595
PflMI	CCANNNNNTGG	1 cut(s)	987
PkrI	GCNGC	4 cut(s)	88, 445, 652, 1186
PleI	GAGTC	1 cut(s)	1460
PpsI	GAGTC	1 cut(s)	1460
PsiI	TTATAA	2 cut(s)	437, 1014
Psp1406I	AACGTT	1 cut(s)	284
Psp6I	CCWGG	3 cut(s)	49, 70, 706
PspEI	GGTNACC	1 cut(s)	858
PspFI	CCCAGC	4 cut(s)	178, 797, 1277, 1309
PspGI	CCWGG	3 cut(s)	49, 70, 706
PspN4I	GGNNCC	3 cut(s)	807, 993, 1467
PspPI	GGNCC	1 cut(s)	992
PsrI	GAACNNNNNNTAC	2 cut(s)	1475, 1507
PstI	CTGCAG	1 cut(s)	1272
PsuI	RGATCY	3 cut(s)	430, 805, 1363
PvuII	CAGCTG	1 cut(s)	1187
RruI	TCGCGA	1 cut(s)	1332
RsaI	GTAC	4 cut(s)	583, 619, 731, 1095
RsaNI	GTAC	4 cut(s)	582, 618, 730, 1094
RseI	CAYNNNNRTG	1 cut(s)	1046
SaqAI	TTAA	2 cut(s)	162, 1053
SatI	GCNGC	4 cut(s)	87, 444, 651, 1185
Sau3AI	GATC	7 cut(s)	155, 319, 325, 430, 781, 805, 1363
Sau96I	GGNCC	1 cut(s)	992
SchI	GAGTC	1 cut(s)	1461
ScrFI	CCNGG	5 cut(s)	51, 72, 708, 1611, 1612
SduI	GDGCHC	4 cut(s)	57, 1041, 1351, 1524
SfaNI	GCATC	4 cut(s)	232, 595, 680, 1264
SfcI	CTRYAG	1 cut(s)	1268
SfuI	TTCGAA	2 cut(s)	1375, 1580
SmaI	CCCGGG	1 cut(s)	1612
SmiMI	CAYNNNNRTG	1 cut(s)	1046
SmlI	CTYRAG	1 cut(s)	1421
SmoI	CTYRAG	1 cut(s)	1421
Sse9I	AATT	9 cut(s)	134, 159, 828, 875, 900, 1062, 1176, 1233, 1584
SsiI	CCGC	2 cut(s)	443, 703
SspMI	CTAG	3 cut(s)	323, 855, 1367
StyD4I	CCNGG	5 cut(s)	49, 70, 706, 1609, 1610
StyI	CCWWGG	2 cut(s)	150, 450
TaaI	ACNGT	8 cut(s)	262, 595, 658, 845, 1010, 1145, 1291, 1532
TaiI	ACGT	4 cut(s)	287, 379, 616, 1619
TaqI	TCGA	4 cut(s)	1026, 1375, 1566, 1580
TasI	AATT	9 cut(s)	134, 159, 828, 875, 900, 1062, 1176, 1233, 1584
TatI	WGTACW	1 cut(s)	729
TauI	GCSGC	1 cut(s)	446
TfiI	GAWTC	3 cut(s)	680, 1325, 1595
Tru1I	TTAA	2 cut(s)	162, 1053
Tru9I	TTAA	2 cut(s)	162, 1053
TscAI	CASTG	6 cut(s)	598, 606, 772, 1117, 1272, 1294
TseFI	GTSAC	7 cut(s)	112, 556, 761, 1115, 1139, 1244, 1283
TseI	GCWGC	3 cut(s)	86, 650, 1184
Tsp45I	GTSAC	7 cut(s)	112, 556, 761, 1115, 1139, 1244, 1283
TspDTI	ATGAA	9 cut(s)	134, 327, 423, 530, 582, 1184, 1236, 1397, 1584
TspGWI	ACGGA	2 cut(s)	504, 1202
TspMI	CCCGGG	1 cut(s)	1610
TspRI	CASTG	6 cut(s)	598, 606, 772, 1117, 1272, 1294
Van91I	CCANNNNNTGG	1 cut(s)	987
VneI	GTGCAC	1 cut(s)	1347
XagI	CCTNNNNNAGG	1 cut(s)	48
XapI	RAATTY	1 cut(s)	875
XbaI	TCTAGA	1 cut(s)	322
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1306
XmaI	CCCGGG	1 cut(s)	1610
XmnI	GAANNNNTTC	2 cut(s)	137, 262
XspI	CTAG	3 cut(s)	323, 855, 1367
Zsp2I	ATGCAT	1 cut(s)	247

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.