FvH4_1g22870

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013821
Physical Location & Seq
Forward (+)
14808270 .. 14810717
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22870.t1

Sequence Viewer

Length: 633 bp
ATGGACAGCTCTATGAAACGAAGAACTTTGCTTAAGGTCATCGTCCTCGGAGATAGTGGGGTGGGAAAGACTTCTTTGATGAATCAATATGTATATAAGAAGTTCATTCGACAATACAAAGCTACAATTGGTGCTGATTTTGTGACAAAGGAGCTTCAAATTGATGACAAATTGGTGACTTTGCAAATTTGGGACACAGCAGGGCAAGAAAGGTTTCATAGTCTCGGGGCGGCATTTTATCGGGGAGCAGATTGCTGTGTTCTTGTTTATGATGTGAATGTTCTAAAATCATTTGAAACACTTCAGAACTGGCATGAAGAGTTTCTCAAACAGGCAGATCCAGCTGATACTGAGGCATTTCCATTTATACTACTGGGCAACAAAATTGATATAGATGGCGGAAACAGCAGAGCGGTTTCTGAGAGGAGAGCCCAAGAATGGTGTGCCGCCAAGGGAAACATACCGTACTTTGAGACATCAGCAAAAGAGGACTATAACGTCGACGAAGCATTTCTCTGTGTAGCAAAAACAGGACTCACCCATGAACATGAACAAGACATTTTCCCCAGTTACTTCAAAGGTATATCAGAAACTGTTTCAGAAGCAGAGCAAAGAGGGGGGTGTGCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

211

Amino Acids

23.63

Weight (kDa)

5.18

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 170 1.1e-12 ADP-ribosylation factor family
Ras PF00071 12 - 177 2.2e-55 Ras family
Roc PF08477 12 - 130 2e-33 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 12 - 109 3e-07 50S ribosome-binding GTPase
Gtr1_RagA PF04670 12 - 133 2.3e-06 Gtr1/RagA G protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013821)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21880 AT2G21880 AT2G21880 AT2G21880
fragaria_vesca FvH4_1g22870 FvH4_1g22870
malus_domestica MD00G1156800.v1.1
prunus_persica Prupe.6G169600_v2.0.a1 Prupe.6G169600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0117211
rosa_laevigata RLG00000018335
rosa_roxburghii Rroxscaffold_2G00126640
rosa_rugosa Rorug02G0211100 Rorug02G0211200
rosa_samantha Rh2AG267000 Rh2DG293300
rosa_wichuraiana Rw2G021060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 497
AccBSI CCGCTC 1 cut(s) 413
AccI GTMKAC 1 cut(s) 501
AciI CCGC 4 cut(s) 230, 399, 413, 447
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 1 cut(s) 186
AcuI CTGAAG 1 cut(s) 287
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 1 cut(s) 438
AflII CTTAAG 1 cut(s) 32
AgsI TTSAA 3 cut(s) 158, 296, 577
AluBI AGCT 4 cut(s) 9, 122, 154, 344
AluI AGCT 4 cut(s) 9, 122, 154, 344
Alw26I GTCTC 2 cut(s) 227, 467
AlwI GGATC 1 cut(s) 332
AlwNI CAGNNNCTG 1 cut(s) 593
Ama87I CYCGRG 1 cut(s) 224
ApoI RAATTY 1 cut(s) 186
Asp700I GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 510
AsuHPI GGTGA 2 cut(s) 187, 529
AvaI CYCGRG 1 cut(s) 224
BanII GRGCYC 1 cut(s) 433
BccI CCATC 1 cut(s) 389
BcoDI GTCTC 2 cut(s) 227, 467
BfrI CTTAAG 1 cut(s) 32
BisI GCNGC 2 cut(s) 231, 447
BlsI GCNGC 2 cut(s) 232, 448
BmeT110I CYCGRG 1 cut(s) 224
BmrI ACTGGG 2 cut(s) 383, 561
BmuI ACTGGG 2 cut(s) 383, 561
BsaJI CCNNGG 2 cut(s) 46, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 438
Bse1I ACTGG 3 cut(s) 314, 378, 567
BseDI CCNNGG 2 cut(s) 46, 450
BseLI CCNNNNNNNGG 1 cut(s) 438
BseMII CTCAG 2 cut(s) 342, 411
BseNI ACTGG 3 cut(s) 314, 378, 567
BseRI GAGGAG 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 438
BsmAI GTCTC 2 cut(s) 227, 467
BsmFI GGGAC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 433
Bsp143I GATC 1 cut(s) 337
BspACI CCGC 4 cut(s) 230, 399, 413, 447
BspCNI CTCAG 2 cut(s) 343, 412
BspPI GGATC 1 cut(s) 332
BspTI CTTAAG 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 413
BsrI ACTGG 3 cut(s) 314, 378, 567
BssECI CCNNGG 2 cut(s) 46, 450
BssMI GATC 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 450
Bst4CI ACNGT 2 cut(s) 465, 595
Bst6I CTCTTC 1 cut(s) 312
BstAFI CTTAAG 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 2 cut(s) 351, 420
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 2 cut(s) 227, 467
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 2 cut(s) 341, 405
BstNSI RCATGY 1 cut(s) 630
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 628
CaiI CAGNNNCTG 1 cut(s) 593
Csp6I GTAC 1 cut(s) 466
CviAII CATG 4 cut(s) 314, 542, 548, 627
CviJI RGCY 5 cut(s) 9, 122, 154, 344, 431
CviKI_1 RGCY 5 cut(s) 9, 122, 154, 344, 431
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 2 cut(s) 351, 420
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
DrdI GACNNNNNNGTC 1 cut(s) 497
DseDI GACNNNNNNGTC 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
EciI GGCGGA 1 cut(s) 414
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 4 cut(s) 317, 545, 551, 630
FaqI GGGAC 1 cut(s) 206
FatI CATG 4 cut(s) 313, 541, 547, 626
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 2 cut(s) 231, 447
FriOI GRGCYC 1 cut(s) 433
Fsp4HI GCNGC 2 cut(s) 231, 447
GluI GCNGC 2 cut(s) 231, 447
Hin1II CATG 4 cut(s) 317, 545, 551, 630
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 2 cut(s) 82, 534
HphI GGTGA 2 cut(s) 187, 529
Hpy166II GTNNAC 1 cut(s) 502
Hpy188I TCNGA 5 cut(s) 50, 306, 421, 589, 601
Hpy8I GTNNAC 1 cut(s) 502
Hpy99I CGWCG 2 cut(s) 503, 506
HpyCH4III ACNGT 2 cut(s) 465, 595
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 2 cut(s) 184, 626
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 405
HpyF3I CTNAG 2 cut(s) 351, 420
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 4 cut(s) 317, 545, 551, 630
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 2 cut(s) 151, 245
LpnPI CCDG 7 cut(s) 186, 295, 317, 354, 359, 516, 580
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 3 cut(s) 142, 175, 569
MalI GATC 1 cut(s) 339
MbiI CCGCTC 1 cut(s) 413
MboI GATC 1 cut(s) 337
MboII GAAGA 2 cut(s) 33, 329
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 433
MluCI AATT 5 cut(s) 126, 159, 170, 186, 384
MlyI GAGTC 1 cut(s) 528
MnlI CCTC 5 cut(s) 56, 346, 417, 481, 608
MroXI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 510
MseI TTAA 1 cut(s) 33
MslI CAYNNNNRTG 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 344
MspCI CTTAAG 1 cut(s) 32
MunI CAATTG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 341, 405
NdeII GATC 1 cut(s) 337
NlaIII CATG 4 cut(s) 317, 545, 551, 630
NmuCI GTSAC 2 cut(s) 142, 175
NspI RCATGY 1 cut(s) 630
PaeI GCATGC 1 cut(s) 630
PdmI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 510
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 2 cut(s) 232, 448
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 546
SalI GTCGAC 1 cut(s) 500
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 2 cut(s) 231, 447
Sau3AI GATC 1 cut(s) 337
SchI GAGTC 1 cut(s) 528
SduI GDGCHC 1 cut(s) 433
SetI ASST 8 cut(s) 11, 39, 124, 156, 215, 346, 501, 583
SgrDI CGTCGACG 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SphI GCATGC 1 cut(s) 630
Sse9I AATT 5 cut(s) 126, 159, 170, 186, 384
SsiI CCGC 4 cut(s) 230, 399, 413, 447
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 2 cut(s) 465, 595
TaiI ACGT 1 cut(s) 501
TaqI TCGA 2 cut(s) 109, 501
TasI AATT 5 cut(s) 126, 159, 170, 186, 384
TauI GCSGC 2 cut(s) 233, 449
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseFI GTSAC 2 cut(s) 142, 175
Tsp45I GTSAC 2 cut(s) 142, 175
TspDTI ATGAA 7 cut(s) 29, 94, 95, 206, 330, 558, 564
Vha464I CTTAAG 1 cut(s) 32
XapI RAATTY 1 cut(s) 186
XceI RCATGY 1 cut(s) 630
XmiI GTMKAC 1 cut(s) 501
XmnI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.