MD00G1156800.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013821
Physical Location & Seq
Reverse (-)
34685405 .. 34688570
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1156800.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGATATTTCTATGAAAAGAAGAACGCTGCTTAAGGTCATCGTCCTTGGAGATAGTGGGGTGGGAAAGACGTCTTTGATGAATCAATATGTTTATAAGAAGTTCAATCGACAATATAAAGCTACAATTGGTGCAGATTTTGTCACCAAGGAACTTCAGATTGATGACAAATTGGTGACCTTGCAAATTTGGGACACAGCAGGGCAAGAAAGATTTCATAGTCTTGGAGCTGCGTTTTATCGCGGATCAGATTGCTGCGTTCTTGTGTATGATGTGAATGTTAATAAATCATTTGAAGCACTTCAGAACTGGCATGAAGAGTTTCTCAAACAGGCAGATCCAACTGACCCAGCAGCATTTCCCTTCATACTACTGGGAAACAAAATTGACATAGATGGTGGAAACAGCCGTGAGGTTTCTGAGAAGAAAGCTAGGGAATGGTGTGCTGCAAAGGGAAACATACCTTACTTTGAGACCTCGGCGAAAGAGGATTACAATGTTGATGAAGCATTTCTATGTGTTGCAAAAACAGGACTAACCAATGAACATGAACAAGACATTTACTTCCAAGGTATTTCAGAAACTGTTTCAGAAGCAGACCAAAGAGGAGTCTGTGCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

208

Amino Acids

23.32

Weight (kDa)

5.07

Isoelectric Point (pI)

28.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 9 - 171 7.3e-13 ADP-ribosylation factor family
Ras PF00071 12 - 177 4.4e-56 Ras family
Roc PF08477 12 - 130 2e-34 Ras of Complex, Roc, domain of DAPkinase
Gtr1_RagA PF04670 12 - 138 2.1e-07 Gtr1/RagA G protein conserved region
MMR_HSR1 PF01926 12 - 111 5e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013821)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21880 AT2G21880 AT2G21880 AT2G21880
fragaria_vesca FvH4_1g22870 FvH4_1g22870
malus_domestica MD00G1156800.v1.1
prunus_persica Prupe.6G169600_v2.0.a1 Prupe.6G169600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0117211
rosa_laevigata RLG00000018335
rosa_roxburghii Rroxscaffold_2G00126640
rosa_rugosa Rorug02G0211100 Rorug02G0211200
rosa_samantha Rh2AG267000 Rh2DG293300
rosa_wichuraiana Rw2G021060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 96
AatII GACGTC 1 cut(s) 74
AccII CGCG 1 cut(s) 243
AciI CCGC 1 cut(s) 243
AclWI GGATC 2 cut(s) 253, 332
AcsI RAATTY 1 cut(s) 186
AcuI CTGAAG 2 cut(s) 140, 287
AcyI GRCGYC 1 cut(s) 71
AflII CTTAAG 1 cut(s) 32
AgsI TTSAA 2 cut(s) 106, 296
AluBI AGCT 3 cut(s) 122, 230, 431
AluI AGCT 3 cut(s) 122, 230, 431
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 2 cut(s) 253, 332
AlwNI CAGNNNCTG 1 cut(s) 584
ApeKI GCWGC 5 cut(s) 28, 230, 255, 353, 446
ApoI RAATTY 1 cut(s) 186
Asp700I GAANNNNTTC 4 cut(s) 213, 300, 321, 510
AsuHPI GGTGA 2 cut(s) 136, 187
BbvI GCAGC 5 cut(s) 15, 217, 242, 365, 433
BccI CCATC 1 cut(s) 389
BceAI ACGGC 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 467
BfaI CTAG 1 cut(s) 432
BfrI CTTAAG 1 cut(s) 32
BisI GCNGC 5 cut(s) 29, 231, 256, 354, 447
BlsI GCNGC 5 cut(s) 30, 232, 257, 355, 448
BmrI ACTGGG 1 cut(s) 383
BmuI ACTGGG 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 4 cut(s) 46, 147, 477, 568
Bse1I ACTGG 2 cut(s) 314, 378
BseDI CCNNGG 4 cut(s) 46, 147, 477, 568
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 2 cut(s) 314, 378
BseRI GAGGAG 1 cut(s) 621
BseXI GCAGC 5 cut(s) 15, 217, 242, 365, 433
BseYI CCCAGC 1 cut(s) 349
BsgI GTGCAG 1 cut(s) 153
Bsh1236I CGCG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 206
Bso31I GGTCTC 1 cut(s) 467
Bsp143I GATC 2 cut(s) 245, 337
BspACI CCGC 1 cut(s) 243
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 243
BspPI GGATC 2 cut(s) 253, 332
BspTI CTTAAG 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 467
BsrI ACTGG 2 cut(s) 314, 378
BssECI CCNNGG 4 cut(s) 46, 147, 477, 568
BssMI GATC 2 cut(s) 245, 337
BssNI GRCGYC 1 cut(s) 71
BssT1I CCWWGG 3 cut(s) 46, 147, 568
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 312
BstACI GRCGYC 1 cut(s) 71
BstAFI CTTAAG 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 619
BstDEI CTNAG 1 cut(s) 420
BstEII GGTNACC 1 cut(s) 175
BstFNI CGCG 1 cut(s) 243
BstKTI GATC 2 cut(s) 248, 340
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 2 cut(s) 245, 337
BstNSI RCATGY 1 cut(s) 621
BstPI GGTNACC 1 cut(s) 175
BstUI CGCG 1 cut(s) 243
BstV1I GCAGC 5 cut(s) 15, 217, 242, 365, 433
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
Cac8I GCNNGC 1 cut(s) 619
CaiI CAGNNNCTG 1 cut(s) 584
CviAII CATG 3 cut(s) 314, 548, 618
CviJI RGCY 4 cut(s) 122, 230, 408, 431
CviKI_1 RGCY 4 cut(s) 122, 230, 408, 431
DdeI CTNAG 1 cut(s) 420
DpnI GATC 2 cut(s) 247, 339
DpnII GATC 2 cut(s) 245, 337
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Eco130I CCWWGG 3 cut(s) 46, 147, 568
Eco31I GGTCTC 1 cut(s) 467
Eco57I CTGAAG 2 cut(s) 140, 287
Eco91I GGTNACC 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 175
EcoT14I CCWWGG 3 cut(s) 46, 147, 568
ErhI CCWWGG 3 cut(s) 46, 147, 568
FaeI CATG 3 cut(s) 317, 551, 621
FaqI GGGAC 1 cut(s) 206
FatI CATG 3 cut(s) 313, 547, 617
Fnu4HI GCNGC 5 cut(s) 29, 231, 256, 354, 447
Fsp4HI GCNGC 5 cut(s) 29, 231, 256, 354, 447
FspBI CTAG 1 cut(s) 432
GluI GCNGC 5 cut(s) 29, 231, 256, 354, 447
GsaI CCCAGC 1 cut(s) 353
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 3 cut(s) 317, 551, 621
HinfI GANTC 2 cut(s) 82, 609
HphI GGTGA 2 cut(s) 136, 187
Hpy188I TCNGA 6 cut(s) 159, 250, 306, 421, 580, 592
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 5 cut(s) 134, 184, 449, 524, 617
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 1 cut(s) 71
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 3 cut(s) 317, 551, 621
Kzo9I GATC 2 cut(s) 245, 337
LmnI GCTCC 1 cut(s) 227
LpnPI CCDG 6 cut(s) 186, 295, 317, 359, 363, 516
Lsp1109I GCAGC 5 cut(s) 15, 217, 242, 365, 433
MaeI CTAG 1 cut(s) 432
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 2 cut(s) 142, 175
MalI GATC 2 cut(s) 247, 339
MboI GATC 2 cut(s) 245, 337
MboII GAAGA 3 cut(s) 33, 329, 436
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 1 cut(s) 337
MluCI AATT 4 cut(s) 126, 170, 186, 384
MlyI GAGTC 1 cut(s) 618
MmeI TCCRAC 1 cut(s) 365
MnlI CCTC 4 cut(s) 406, 481, 487, 599
MroXI GAANNNNTTC 4 cut(s) 213, 300, 321, 510
MseI TTAA 2 cut(s) 33, 282
MslI CAYNNNNRTG 1 cut(s) 514
MspCI CTTAAG 1 cut(s) 32
MunI CAATTG 1 cut(s) 126
MvnI CGCG 1 cut(s) 243
NdeII GATC 2 cut(s) 245, 337
NlaIII CATG 3 cut(s) 317, 551, 621
NmeAIII GCCGAG 1 cut(s) 458
NmuCI GTSAC 2 cut(s) 142, 175
NspI RCATGY 1 cut(s) 621
PaeI GCATGC 1 cut(s) 621
PdmI GAANNNNTTC 4 cut(s) 213, 300, 321, 510
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 5 cut(s) 30, 232, 257, 355, 448
PleI GAGTC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 617
PsiI TTATAA 1 cut(s) 96
PspEI GGTNACC 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 349
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 514
SaqAI TTAA 2 cut(s) 33, 282
SatI GCNGC 5 cut(s) 29, 231, 256, 354, 447
Sau3AI GATC 2 cut(s) 245, 337
SchI GAGTC 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 514
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SphI GCATGC 1 cut(s) 621
Sse9I AATT 4 cut(s) 126, 170, 186, 384
SsiI CCGC 1 cut(s) 243
SspMI CTAG 1 cut(s) 432
StyI CCWWGG 3 cut(s) 46, 147, 568
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 1 cut(s) 74
TaqI TCGA 1 cut(s) 109
TasI AATT 4 cut(s) 126, 170, 186, 384
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 2 cut(s) 33, 282
Tru9I TTAA 2 cut(s) 33, 282
TseFI GTSAC 2 cut(s) 142, 175
TseI GCWGC 5 cut(s) 28, 230, 255, 353, 446
Tsp45I GTSAC 2 cut(s) 142, 175
TspDTI ATGAA 8 cut(s) 29, 95, 206, 330, 355, 519, 558, 564
Vha464I CTTAAG 1 cut(s) 32
XapI RAATTY 1 cut(s) 186
XceI RCATGY 1 cut(s) 621
XmnI GAANNNNTTC 4 cut(s) 213, 300, 321, 510
XspI CTAG 1 cut(s) 432
ZraI GACGTC 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.