Rroxscaffold_2G00126640

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013821
Physical Location & Seq
Reverse (-)
61987765 .. 61990381
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126640.1

Sequence Viewer

Length: 639 bp
ATGGATGTTTCTATGAAACGAAGAACGCTGCTTAAGGTCATCGTCCTCGGAGATAGTGGGGTGGGAAAGACTTCTTTGATGAATCAATATGTATATAAGAAGTTCATTCGACATTATAAAGCTACTATCGGTGCTGATTTTGTCACAAAGGAACTTCATATTGATGACAAAGTGGTGACTTTGCAAATTTGGGACACAGCAGGACAAGAAAGGTTTCAAAGTCTCGGGGCAGCATTTTATCGTGGAGCAGATTGCTGTGTTCTTGTTTATGATGTGAATGTTCTAAGATCATTTGAAACACTTCAAAACTGGCATGAAGAGTTTCTCAAACAGCAGGTAGATCCAGCTGACACTGAGGCATTTCCATTTATACTAATCGGCAACAAAACTGATATAGATGGCGGAAACAGCAGAGCGGTTTCTAAGAAGAGAGCCCAAGAATGGTGTGCTGCCAAGGGAAACATACCGTACTTTGAGACATCAGCAAAAGAGGATTATAACGTTGATGAAGCATTTCTCTGTGTAGCAAAAACAGGACTCACCCATGAACATGAACAAGACATTTTCCCATGCAGTTACTTCAAAGGTATATCAGAAACTGTTTCAGAAGCAGAGCAAAGAGGGGGCTGTGCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

212

Amino Acids

23.89

Weight (kDa)

5.58

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 172 1.6e-11 ADP-ribosylation factor family
Ras PF00071 12 - 178 4.3e-55 Ras family
Roc PF08477 12 - 131 4.6e-33 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 12 - 110 3.2e-07 50S ribosome-binding GTPase
Gtr1_RagA PF04670 12 - 131 1.3e-06 Gtr1/RagA G protein conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013821)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21880 AT2G21880 AT2G21880 AT2G21880
fragaria_vesca FvH4_1g22870 FvH4_1g22870
malus_domestica MD00G1156800.v1.1
prunus_persica Prupe.6G169600_v2.0.a1 Prupe.6G169600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0117211
rosa_laevigata RLG00000018335
rosa_roxburghii Rroxscaffold_2G00126640
rosa_rugosa Rorug02G0211100 Rorug02G0211200
rosa_samantha Rh2AG267000 Rh2DG293300
rosa_wichuraiana Rw2G021060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 117, 498
Acc36I ACCTGC 1 cut(s) 325
AccBSI CCGCTC 1 cut(s) 416
AciI CCGC 2 cut(s) 402, 416
AclI AACGTT 1 cut(s) 501
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 1 cut(s) 470
AfiI CCNNNNNNNGG 1 cut(s) 441
AflII CTTAAG 1 cut(s) 32
AgsI TTSAA 4 cut(s) 218, 296, 305, 583
AluBI AGCT 2 cut(s) 122, 347
AluI AGCT 2 cut(s) 122, 347
Alw26I GTCTC 2 cut(s) 227, 470
AlwI GGATC 1 cut(s) 335
AlwNI CAGNNNCTG 1 cut(s) 599
Ama87I CYCGRG 1 cut(s) 224
ApeKI GCWGC 3 cut(s) 28, 230, 449
ApoI RAATTY 1 cut(s) 186
Asp700I GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 513
AsuHPI GGTGA 2 cut(s) 187, 532
AvaI CYCGRG 1 cut(s) 224
BanII GRGCYC 1 cut(s) 436
BbvI GCAGC 3 cut(s) 15, 242, 436
BccI CCATC 1 cut(s) 392
BcoDI GTCTC 2 cut(s) 227, 470
BfrI CTTAAG 1 cut(s) 32
BfuAI ACCTGC 1 cut(s) 325
BisI GCNGC 3 cut(s) 29, 231, 450
BlsI GCNGC 3 cut(s) 30, 232, 451
BmeT110I CYCGRG 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 46, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 441
Bse1I ACTGG 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 46, 453
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 441
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 1 cut(s) 314
BseXI GCAGC 3 cut(s) 15, 242, 436
BsiHKCI CYCGRG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 441
BsmAI GTCTC 2 cut(s) 227, 470
BsmFI GGGAC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 2 cut(s) 287, 340
BspACI CCGC 2 cut(s) 402, 416
BspCNI CTCAG 1 cut(s) 346
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 1 cut(s) 335
BspTI CTTAAG 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 416
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 46, 453
BssMI GATC 2 cut(s) 287, 340
BssT1I CCWWGG 1 cut(s) 453
Bst4CI ACNGT 2 cut(s) 468, 601
Bst6I CTCTTC 2 cut(s) 312, 422
BstAFI CTTAAG 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 634
BstDEI CTNAG 3 cut(s) 284, 354, 423
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 2 cut(s) 290, 343
BstMAI GTCTC 2 cut(s) 227, 470
BstMBI GATC 2 cut(s) 287, 340
BstMWI GCNNNNNNNGC 2 cut(s) 408, 633
BstNSI RCATGY 1 cut(s) 636
BstV1I GCAGC 3 cut(s) 15, 242, 436
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 351
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 634
CaiI CAGNNNCTG 1 cut(s) 599
Csp6I GTAC 1 cut(s) 469
CviAII CATG 5 cut(s) 314, 545, 551, 570, 633
CviJI RGCY 4 cut(s) 122, 347, 434, 627
CviKI_1 RGCY 4 cut(s) 122, 347, 434, 627
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 3 cut(s) 284, 354, 423
DpnI GATC 2 cut(s) 289, 342
DpnII GATC 2 cut(s) 287, 340
Eam1104I CTCTTC 2 cut(s) 312, 422
EarI CTCTTC 2 cut(s) 312, 422
EciI GGCGGA 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 5 cut(s) 317, 548, 554, 573, 636
FaqI GGGAC 1 cut(s) 206
FatI CATG 5 cut(s) 313, 544, 550, 569, 632
Fnu4HI GCNGC 3 cut(s) 29, 231, 450
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 436
Fsp4HI GCNGC 3 cut(s) 29, 231, 450
GluI GCNGC 3 cut(s) 29, 231, 450
Hin1II CATG 5 cut(s) 317, 548, 554, 573, 636
HinfI GANTC 2 cut(s) 82, 537
HphI GGTGA 2 cut(s) 187, 532
Hpy188I TCNGA 3 cut(s) 50, 595, 607
HpyCH4III ACNGT 2 cut(s) 468, 601
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 3 cut(s) 184, 573, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 408, 633
HpyF3I CTNAG 3 cut(s) 284, 354, 423
HpySE526I ACGT 1 cut(s) 501
Hsp92II CATG 5 cut(s) 317, 548, 554, 573, 636
Kzo9I GATC 2 cut(s) 287, 340
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 5 cut(s) 186, 295, 320, 357, 519
Lsp1109I GCAGC 3 cut(s) 15, 242, 436
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 3 cut(s) 142, 175, 575
MalI GATC 2 cut(s) 289, 342
MbiI CCGCTC 1 cut(s) 416
MboI GATC 2 cut(s) 287, 340
MboII GAAGA 3 cut(s) 33, 329, 439
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 1 cut(s) 186
MlyI GAGTC 1 cut(s) 531
MnlI CCTC 4 cut(s) 56, 349, 484, 614
MroXI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 513
MseI TTAA 1 cut(s) 33
MslI CAYNNNNRTG 2 cut(s) 162, 549
MspA1I CMGCKG 1 cut(s) 347
MspCI CTTAAG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 408, 633
NdeII GATC 2 cut(s) 287, 340
NlaIII CATG 5 cut(s) 317, 548, 554, 573, 636
NmuCI GTSAC 2 cut(s) 142, 175
NspI RCATGY 1 cut(s) 636
PaeI GCATGC 1 cut(s) 636
PdmI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 513
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 3 cut(s) 30, 232, 451
PleI GAGTC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 531
PsiI TTATAA 2 cut(s) 117, 498
Psp1406I AACGTT 1 cut(s) 501
PstNI CAGNNNCTG 1 cut(s) 599
PsuI RGATCY 1 cut(s) 340
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 2 cut(s) 162, 549
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 3 cut(s) 29, 231, 450
Sau3AI GATC 2 cut(s) 287, 340
SchI GAGTC 1 cut(s) 531
SduI GDGCHC 1 cut(s) 436
SetI ASST 7 cut(s) 39, 124, 215, 339, 349, 504, 589
SmiMI CAYNNNNRTG 2 cut(s) 162, 549
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SphI GCATGC 1 cut(s) 636
Sse9I AATT 1 cut(s) 186
SsiI CCGC 2 cut(s) 402, 416
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 2 cut(s) 468, 601
TaiI ACGT 1 cut(s) 504
TaqI TCGA 1 cut(s) 109
TasI AATT 1 cut(s) 186
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 2 cut(s) 142, 175
TseI GCWGC 3 cut(s) 28, 230, 449
Tsp45I GTSAC 2 cut(s) 142, 175
TspDTI ATGAA 8 cut(s) 29, 94, 95, 146, 330, 522, 561, 567
TspRI CASTG 1 cut(s) 358
Vha464I CTTAAG 1 cut(s) 32
XapI RAATTY 1 cut(s) 186
XceI RCATGY 1 cut(s) 636
XmnI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.