Rh2DG293300

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013821
Physical Location & Seq
Forward (+)
34524251 .. 34526382
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG293300.1

Sequence Viewer

Length: 624 bp
ATGGATGTTTCTATGAAACGAAGAACGCTGCTTAAGGTCATCGTCCTCGGAGATAGCGGGGTGGGAAAGACTTCTTTGATGAATCAATATGTATATGAGAAGTTCATTCGACATTATAAAGCTACAATCGGTGCTGATTTTGTCACAAAGGAACTTCATATTGATGACAAAGTGGTGACTTTGCAAATTTGGGACACAGCAGGACAAGAAAGGTTTCAAAGTCTCGGGGCAGCATTTTATCGTGGAGCAGATTGCTGTGTTCTTGTTTATGATGTGAATGTTCTACGATCATTTGAAACACTTCAAAACTGGCATGAAGAGTTTCTTAAACAGGTAGATCCAGCTGACACTGAGGCATTTCCATTTATACTAATCGGCAACAAAATTGATATAGATGGCGGAAACAGCAGAGCGGTTTCTAAGAAGAGAGCCCAAGAATGGTGTGCTGCCAAGGGAAACATACCGTACTTTGAGACATCAGCAAAAGAGGATTATAACGTTGATGAAGCATTTCTGTGTGTAGCAAAAACAGGACTCACCCATGAACATGAACAAGACATTTACTTCAAAGGTATATCAGAAACTGTTTCAGAAGCAGAGCAAAGAGGGGGCTGTGCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04138 ko04140 ko04144 ko04145 ko05132 ko05146 ko05152 map04137 map04138 map04140 map04144 map04145 map05132 map05146 map05152
Pfam Domains
Protein Families
Ras

Protein Analysis

207

Amino Acids

23.34

Weight (kDa)

5.29

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 171 1.7e-12 ADP-ribosylation factor family
Ras PF00071 12 - 177 1.1e-55 Ras family
Roc PF08477 12 - 130 1.1e-33 Ras of Complex, Roc, domain of DAPkinase
Gtr1_RagA PF04670 12 - 140 1.8e-07 Gtr1/RagA G protein conserved region
MMR_HSR1 PF01926 12 - 110 3.5e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013821)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21880 AT2G21880 AT2G21880 AT2G21880
fragaria_vesca FvH4_1g22870 FvH4_1g22870
malus_domestica MD00G1156800.v1.1
prunus_persica Prupe.6G169600_v2.0.a1 Prupe.6G169600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0117211
rosa_laevigata RLG00000018335
rosa_roxburghii Rroxscaffold_2G00126640
rosa_rugosa Rorug02G0211100 Rorug02G0211200
rosa_samantha Rh2AG267000 Rh2DG293300
rosa_wichuraiana Rw2G021060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 117, 495
AccBSI CCGCTC 1 cut(s) 413
AciI CCGC 3 cut(s) 57, 399, 413
AclI AACGTT 1 cut(s) 498
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 1 cut(s) 438
AflII CTTAAG 1 cut(s) 32
AgsI TTSAA 4 cut(s) 218, 296, 305, 568
AluBI AGCT 2 cut(s) 122, 344
AluI AGCT 2 cut(s) 122, 344
Alw26I GTCTC 2 cut(s) 227, 467
AlwI GGATC 1 cut(s) 332
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 224
ApeKI GCWGC 3 cut(s) 28, 230, 446
ApoI RAATTY 1 cut(s) 186
Asp700I GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 510
AsuHPI GGTGA 2 cut(s) 187, 529
AvaI CYCGRG 1 cut(s) 224
BanII GRGCYC 1 cut(s) 433
BbvI GCAGC 3 cut(s) 15, 242, 433
BccI CCATC 1 cut(s) 389
BcoDI GTCTC 2 cut(s) 227, 467
BfrI CTTAAG 1 cut(s) 32
BisI GCNGC 3 cut(s) 29, 231, 447
BlsI GCNGC 3 cut(s) 30, 232, 448
BmeT110I CYCGRG 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 46, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 438
Bse1I ACTGG 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 46, 450
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 1 cut(s) 314
BseXI GCAGC 3 cut(s) 15, 242, 433
BsiHKCI CYCGRG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 438
BsmAI GTCTC 2 cut(s) 227, 467
BsmFI GGGAC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 433
Bsp143I GATC 2 cut(s) 287, 337
BspACI CCGC 3 cut(s) 57, 399, 413
BspCNI CTCAG 1 cut(s) 343
BspPI GGATC 1 cut(s) 332
BspTI CTTAAG 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 413
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 46, 450
BssMI GATC 2 cut(s) 287, 337
BssT1I CCWWGG 1 cut(s) 450
Bst4CI ACNGT 2 cut(s) 465, 586
Bst6I CTCTTC 2 cut(s) 312, 419
BstAFI CTTAAG 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 619
BstDEI CTNAG 2 cut(s) 351, 420
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 2 cut(s) 290, 340
BstMAI GTCTC 2 cut(s) 227, 467
BstMBI GATC 2 cut(s) 287, 337
BstMWI GCNNNNNNNGC 2 cut(s) 405, 618
BstNSI RCATGY 1 cut(s) 621
BstV1I GCAGC 3 cut(s) 15, 242, 433
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 1 cut(s) 619
CaiI CAGNNNCTG 1 cut(s) 584
Csp6I GTAC 1 cut(s) 466
CviAII CATG 4 cut(s) 314, 542, 548, 618
CviJI RGCY 4 cut(s) 122, 344, 431, 612
CviKI_1 RGCY 4 cut(s) 122, 344, 431, 612
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 2 cut(s) 351, 420
DpnI GATC 2 cut(s) 289, 339
DpnII GATC 2 cut(s) 287, 337
Eam1104I CTCTTC 2 cut(s) 312, 419
EarI CTCTTC 2 cut(s) 312, 419
EciI GGCGGA 1 cut(s) 414
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 4 cut(s) 317, 545, 551, 621
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 206
FatI CATG 4 cut(s) 313, 541, 547, 617
FauI CCCGC 1 cut(s) 50
Fnu4HI GCNGC 3 cut(s) 29, 231, 447
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 433
Fsp4HI GCNGC 3 cut(s) 29, 231, 447
GluI GCNGC 3 cut(s) 29, 231, 447
Hin1II CATG 4 cut(s) 317, 545, 551, 621
HinfI GANTC 2 cut(s) 82, 534
HphI GGTGA 2 cut(s) 187, 529
Hpy188I TCNGA 3 cut(s) 50, 580, 592
HpyCH4III ACNGT 2 cut(s) 465, 586
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 2 cut(s) 184, 617
HpyF10VI GCNNNNNNNGC 2 cut(s) 405, 618
HpyF3I CTNAG 2 cut(s) 351, 420
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 4 cut(s) 317, 545, 551, 621
Kzo9I GATC 2 cut(s) 287, 337
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 5 cut(s) 186, 295, 317, 354, 516
Lsp1109I GCAGC 3 cut(s) 15, 242, 433
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 2 cut(s) 142, 175
MalI GATC 2 cut(s) 289, 339
MbiI CCGCTC 1 cut(s) 413
MboI GATC 2 cut(s) 287, 337
MboII GAAGA 3 cut(s) 33, 329, 436
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 433
MluCI AATT 2 cut(s) 186, 384
MlyI GAGTC 1 cut(s) 528
MnlI CCTC 4 cut(s) 56, 346, 481, 599
MroXI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 510
MseI TTAA 2 cut(s) 33, 327
MslI CAYNNNNRTG 3 cut(s) 162, 514, 546
MspA1I CMGCKG 1 cut(s) 344
MspCI CTTAAG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 405, 618
NdeII GATC 2 cut(s) 287, 337
NlaIII CATG 4 cut(s) 317, 545, 551, 621
NmuCI GTSAC 2 cut(s) 142, 175
NspI RCATGY 1 cut(s) 621
PaeI GCATGC 1 cut(s) 621
PdmI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 510
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 3 cut(s) 30, 232, 448
PleI GAGTC 1 cut(s) 528
PpsI GAGTC 1 cut(s) 528
PsiI TTATAA 2 cut(s) 117, 495
Psp1406I AACGTT 1 cut(s) 498
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 3 cut(s) 162, 514, 546
SaqAI TTAA 2 cut(s) 33, 327
SatI GCNGC 3 cut(s) 29, 231, 447
Sau3AI GATC 2 cut(s) 287, 337
SchI GAGTC 1 cut(s) 528
SduI GDGCHC 1 cut(s) 433
SetI ASST 7 cut(s) 39, 124, 215, 336, 346, 501, 574
SmiMI CAYNNNNRTG 3 cut(s) 162, 514, 546
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SphI GCATGC 1 cut(s) 621
Sse9I AATT 2 cut(s) 186, 384
SsiI CCGC 3 cut(s) 57, 399, 413
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 2 cut(s) 465, 586
TaiI ACGT 1 cut(s) 501
TaqI TCGA 1 cut(s) 109
TasI AATT 2 cut(s) 186, 384
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 2 cut(s) 33, 327
Tru9I TTAA 2 cut(s) 33, 327
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 2 cut(s) 142, 175
TseI GCWGC 3 cut(s) 28, 230, 446
Tsp45I GTSAC 2 cut(s) 142, 175
TspDTI ATGAA 8 cut(s) 29, 94, 95, 146, 330, 519, 558, 564
TspRI CASTG 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 32
XapI RAATTY 1 cut(s) 186
XceI RCATGY 1 cut(s) 621
XmnI GAANNNNTTC 5 cut(s) 70, 213, 300, 321, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.