FvH4_1g23600
MYB Family

Trihelix transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017230
Physical Location & Seq
Forward (+)
15427540 .. 15430066
2527 bp
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UTR
Exon/CDS
Intron
FvH4_1g23600.t2

Sequence Viewer

Length: 1695 bp
ATGCTGGAAAATTCAACTTTCCCGGTCAATCCAAGCTCACCCACAACCGACCGGAGTGCCGAGGAGGTCGCCGGCGATGGTGGGTCGGTCTCAGTTGGGCTCGAGGAGGAAGAGAGAGCTGGGCTGGAGCAGGGTTACCGGAACTGGGCCGGTAACCGTTGGCCTCGGCCGGAGACGCTGGCTCTGCTGAAGATAAGGTCAGATATGGACGCGGAGTTCAAGGCAGCCAGCCCTAAATTGCCTCTCTGGGAAGAGGTTTCCAGGAAAATGGGAGAGGCTGGATATAGTAGAGACGCCAAAAAATGCAAGGAGAAATTTGAGAACATATACAAGTACCACAAAAGGACTAGAGAATCACGGGGTGCTCGATCAGCTGGCAAGACATATAAGTACTTTGAGCAATTGGAGGCTATAGAACAACACCATTCTGAACATCCTCCTCCCGCTGAAACAGTTCAAGCTTCTACAGCTGAAACAGCAGCAGCGAGCCCAAAGGATGGTGTTTCAAATGCAATCCCGTGTTCTTCGATGCAACACCAGCTTTCTAGTTTTGTTGACAACTCGACTCCCACCACATCGTGTTCAAGTAAAGAGTCTGAAGGCACGCACAAGAAGAAGAGGAGGGTGACTGAGTTCTTTGAGAAGTTGATGAAGCAAGTGATCGACAAGCAAGAGAATTTGCAGACCAAGTTTGTGGAGGTGTTGGACAAGTATGAACAAGAACGGATAGCTAGAGAAGAGGCGTGGAAGATGCAAGAATTGACTAGAATTGAGAGAGAAAGAGAGCTCCTAGCTCAAGAGAGGTCTGTTGCAGCTGCAAAAGATGCTGCTGTTCTTGCATTTTTGCAAAAGTTTTCAGAACAAGCAGCCTCAGTGAATTTGCCAAGCCAATCATGCTCAGTTCAAATTCCAAATCAACCGTCCTCTGTGCATTTGCCATTGCAAGCGTGTTCAGTTCAATTACCAAATCAAGTATCCTCGGCGCAACTGCCGGTGCAAGCATCTGCAGTCCGAGTTCAAATGCCTGCTAACTCATCTGTGGCCAATACAGTTTCTGAAAAGCAGAAAAAGGATGAAGATTGGGAGCTTGTGCGTAGGAGTTTGGAATCGCCAGACAAGAATAATGGTAGAAGTCGTACACCTACTAATTCTACAAGGTGGCCAAGAGAAGAAGTTTCAGATTTGATTAGGTTGAGATCTAATTATGATTTGCAATATCAAGAAAATGGAAGTAAAGGCCATTTGTGGGAGGAGATCGCAGCATCAATGAAAAAGCTTGGTTATGACCGGAATGCCAAAAGGTGCAAGGAGAAGTGGGAGAACATAAACAAGTACTACAGAAGATTGAAAGATAGCAACAAGAAAAGGCCCGAGGATTCAAAGACATGTGCTTATGTTGAGATGCTTGATTCTTTGTACAACAAGAAGAATAGTAAGGGTGAGAATCAAGCTGATAAGAATTATGATTTGAGGCCTGAGGAGCTACTGATGCATATGATGAGTGGGCAAGAAGAGCAGCAGCAGCAGCCAGAATCAGCGACTCAGAATGGTGATAGTCAGAACGTTGAGAAGATTCAAGGAGATAATGGAAGCATAGAGGGAGTGGGAGATGTTGCTGGAGATGGTTATCGGATTGTGGCTGCTAATCCTTCTCCAATGGAACAAGATAATCCTTCAGTGGCAATTATGAGTTGA

Protein Analysis

565

Amino Acids

63.61

Weight (kDa)

6.13

Isoelectric Point (pI)

67.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-bind_4 PF13837 52 - 138 1.3e-20 Myb/SANT-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017230)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23600 FvH4_1g23600
prunus_persica Prupe.6G162600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0119181
rosa_laevigata RLG00000018449
rosa_multiflora Rmu_sc0000184.1_g000013
rosa_roxburghii Rroxscaffold_2G00124470
rosa_rugosa Rorug02G0220700
rosa_samantha Rh2AG278600 Rh2BG289500 Rh2DG284700 Rh2DG303700
rosa_wichuraiana Rw2G022140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 497
AccII CGCG 1 cut(s) 212
AciI CCGC 2 cut(s) 212, 444
AclI AACGTT 1 cut(s) 1563
AcoI YGGCCR 3 cut(s) 167, 1041, 1160
AcsI RAATTY 5 cut(s) 10, 314, 676, 877, 906
AcuI CTGAAG 3 cut(s) 209, 618, 1659
AcyI GRCGYC 1 cut(s) 294
AdeI CACNNNGTG 2 cut(s) 362, 579
AfaI GTAC 5 cut(s) 335, 392, 1138, 1334, 1418
AfiI CCNNNNNNNGG 3 cut(s) 145, 497, 1246
AflIII ACRYGT 1 cut(s) 1385
AjnI CCWGG 1 cut(s) 260
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
Alw21I GWGCWC 2 cut(s) 367, 789
Alw26I GTCTC 3 cut(s) 94, 167, 285
AlwNI CAGNNNCTG 1 cut(s) 1055
Ama87I CYCGRG 2 cut(s) 101, 1370
AoxI GGCC 8 cut(s) 147, 161, 167, 1041, 1160, 1237, 1367, 1472
ApoI RAATTY 5 cut(s) 10, 314, 676, 877, 906
ArsI GACNNNNNNTTYG 2 cut(s) 905, 937
Asp700I GAANNNNTTC 1 cut(s) 453
AspLEI GCGC 1 cut(s) 985
AspS9I GGNCC 2 cut(s) 147, 1368
AsuC2I CCSGG 1 cut(s) 23
AsuHPI GGTGA 4 cut(s) 30, 637, 1451, 1562
AvaI CYCGRG 2 cut(s) 101, 1370
AxyI CCTNAGG 1 cut(s) 1476
BalI TGGCCA 2 cut(s) 1043, 1162
BanII GRGCYC 3 cut(s) 102, 491, 789
Bbv12I GWGCWC 2 cut(s) 367, 789
BccI CCATC 3 cut(s) 71, 491, 1616
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 1 cut(s) 262
BciVI GTATCC 1 cut(s) 985
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 3 cut(s) 94, 167, 285
BfaI CTAG 5 cut(s) 348, 546, 732, 765, 791
BfmI CTRYAG 4 cut(s) 411, 465, 1005, 1336
BfuI GTATCC 1 cut(s) 985
BglII AGATCT 1 cut(s) 1196
BmcAI AGTACT 2 cut(s) 392, 1334
Bme1390I CCNGG 2 cut(s) 23, 262
BmeT110I CYCGRG 2 cut(s) 101, 1370
BmgT120I GGNCC 2 cut(s) 147, 1368
BmrFI CCNGG 2 cut(s) 23, 262
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 7 cut(s) 519, 741, 814, 1010, 1271, 1392, 1479
BmuI ACTGGG 1 cut(s) 154
BpmI CTGGAG 2 cut(s) 146, 1638
BpuEI CTTGAG 1 cut(s) 780
BpuMI CCSGG 1 cut(s) 23
BsaBI GATNNNNATC 1 cut(s) 1626
BsaHI GRCGYC 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 4 cut(s) 60, 164, 978, 1371
BsaWI WCCGGW 3 cut(s) 51, 138, 1287
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 497, 1246
Bse118I RCCGGY 3 cut(s) 71, 149, 991
Bse1I ACTGG 1 cut(s) 149
Bse21I CCTNAGG 1 cut(s) 1476
Bse3DI GCAATG 1 cut(s) 938
Bse8I GATNNNNATC 1 cut(s) 1626
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 4 cut(s) 60, 164, 978, 1371
BseGI GGATG 3 cut(s) 433, 502, 1078
BseJI GATNNNNATC 1 cut(s) 1626
BseLI CCNNNNNNNGG 3 cut(s) 145, 497, 1246
BseMI GCAATG 1 cut(s) 938
BseMII CTCAG 6 cut(s) 105, 621, 885, 912, 1467, 1556
BseNI ACTGG 1 cut(s) 149
BseRI GAGGAG 6 cut(s) 77, 119, 429, 634, 1265, 1493
BseX3I CGGCCG 1 cut(s) 167
BseYI CCCAGC 1 cut(s) 119
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 2 cut(s) 52, 170
BshFI GGCC 8 cut(s) 149, 163, 169, 1043, 1162, 1239, 1369, 1474
BsiEI CGRYCG 2 cut(s) 52, 170
BsiHKAI GWGCWC 2 cut(s) 367, 789
BsiHKCI CYCGRG 2 cut(s) 101, 1370
BsiSI CCGG 8 cut(s) 23, 52, 72, 139, 150, 170, 992, 1288
BslI CCNNNNNNNGG 3 cut(s) 145, 497, 1246
BsmAI GTCTC 3 cut(s) 94, 167, 285
BsmBI CGTCTC 2 cut(s) 167, 285
BsmI GAATGC 1 cut(s) 1297
BsnI GGCC 8 cut(s) 149, 163, 169, 1043, 1162, 1239, 1369, 1474
Bso31I GGTCTC 1 cut(s) 94
BsoBI CYCGRG 2 cut(s) 101, 1370
Bsp1286I GDGCHC 4 cut(s) 102, 367, 491, 789
Bsp1407I TGTACA 1 cut(s) 1416
Bsp143I GATC 4 cut(s) 368, 660, 1196, 1254
BspACI CCGC 2 cut(s) 212, 444
BspANI GGCC 8 cut(s) 149, 163, 169, 1043, 1162, 1239, 1369, 1474
BspCNI CTCAG 6 cut(s) 104, 622, 884, 911, 1468, 1555
BspFNI CGCG 1 cut(s) 212
BspMAI CTGCAG 1 cut(s) 1009
BspQI GCTCTTC 1 cut(s) 1506
BspTNI GGTCTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 938
BsrFI RCCGGY 3 cut(s) 71, 149, 991
BsrGI TGTACA 1 cut(s) 1416
BsrI ACTGG 1 cut(s) 149
BssAI RCCGGY 3 cut(s) 71, 149, 991
BssECI CCNNGG 4 cut(s) 60, 164, 978, 1371
BssMI GATC 4 cut(s) 368, 660, 1196, 1254
BssNI GRCGYC 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 4 cut(s) 158, 454, 921, 1051
Bst6I CTCTTC 5 cut(s) 105, 246, 611, 732, 1506
BstACI GRCGYC 1 cut(s) 294
BstAPI GCANNNNNTGC 1 cut(s) 824
BstAUI TGTACA 1 cut(s) 1416
BstC8I GCNNGC 9 cut(s) 73, 180, 229, 376, 487, 605, 945, 999, 1026
BstDEI CTNAG 6 cut(s) 91, 630, 871, 898, 1476, 1542
BstEII GGTNACC 2 cut(s) 134, 152
BstF5I GGATG 3 cut(s) 433, 502, 1078
BstFNI CGCG 1 cut(s) 212
BstHHI GCGC 1 cut(s) 985
BstKTI GATC 4 cut(s) 371, 663, 1199, 1257
BstMAI GTCTC 3 cut(s) 94, 167, 285
BstMBI GATC 4 cut(s) 368, 660, 1196, 1254
BstMCI CGRYCG 2 cut(s) 52, 170
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 1 cut(s) 1389
BstPI GGTNACC 2 cut(s) 134, 152
BstSCI CCNGG 2 cut(s) 21, 260
BstSFI CTRYAG 4 cut(s) 411, 465, 1005, 1336
BstUI CGCG 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 1196
BstXI CCANNNNNNTGG 2 cut(s) 268, 694
BstYI RGATCY 1 cut(s) 1196
BstZI CGGCCG 1 cut(s) 167
Bsu36I CCTNAGG 1 cut(s) 1476
BsuI GTATCC 1 cut(s) 985
BsuRI GGCC 8 cut(s) 149, 163, 169, 1043, 1162, 1239, 1369, 1474
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 3 cut(s) 433, 502, 1078
BtsIMutI CAGTG 2 cut(s) 879, 1683
Cac8I GCNNGC 9 cut(s) 73, 180, 229, 376, 487, 605, 945, 999, 1026
CaiI CAGNNNCTG 1 cut(s) 1055
CfoI GCGC 1 cut(s) 985
Cfr10I RCCGGY 3 cut(s) 71, 149, 991
Cfr13I GGNCC 2 cut(s) 147, 1368
CseI GACGC 3 cut(s) 184, 218, 302
Csp6I GTAC 5 cut(s) 334, 391, 1137, 1333, 1417
CviAII CATG 2 cut(s) 894, 1386
CviQI GTAC 5 cut(s) 334, 391, 1137, 1333, 1417
DdeI CTNAG 6 cut(s) 91, 630, 871, 898, 1476, 1542
DpnI GATC 4 cut(s) 370, 662, 1198, 1256
DpnII GATC 4 cut(s) 368, 660, 1196, 1254
DraIII CACNNNGTG 2 cut(s) 362, 579
EaeI YGGCCR 3 cut(s) 167, 1041, 1160
EagI CGGCCG 1 cut(s) 167
Eam1104I CTCTTC 5 cut(s) 105, 246, 611, 732, 1506
EarI CTCTTC 5 cut(s) 105, 246, 611, 732, 1506
Ecl136II GAGCTC 1 cut(s) 787
EclXI CGGCCG 1 cut(s) 167
Eco147I AGGCCT 1 cut(s) 1474
Eco24I GRGCYC 3 cut(s) 102, 491, 789
Eco31I GGTCTC 1 cut(s) 94
Eco52I CGGCCG 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 787
Eco57I CTGAAG 3 cut(s) 209, 618, 1659
Eco81I CCTNAGG 1 cut(s) 1476
Eco88I CYCGRG 2 cut(s) 101, 1370
Eco91I GGTNACC 2 cut(s) 134, 152
EcoICRI GAGCTC 1 cut(s) 787
EcoO65I GGTNACC 2 cut(s) 134, 152
EcoRII CCWGG 1 cut(s) 260
EcoT22I ATGCAT 1 cut(s) 1494
EcoT38I GRGCYC 3 cut(s) 102, 491, 789
Esp3I CGTCTC 2 cut(s) 167, 285
FaeI CATG 2 cut(s) 897, 1389
FatI CATG 2 cut(s) 893, 1385
FauI CCCGC 1 cut(s) 451
FauNDI CATATG 1 cut(s) 1494
FokI GGATG 3 cut(s) 420, 509, 1085
FriOI GRGCYC 3 cut(s) 102, 491, 789
FspBI CTAG 5 cut(s) 348, 546, 732, 765, 791
GlaI GCGC 1 cut(s) 984
GsaI CCCAGC 1 cut(s) 123
GsuI CTGGAG 2 cut(s) 146, 1638
HaeIII GGCC 8 cut(s) 149, 163, 169, 1043, 1162, 1239, 1369, 1474
HapII CCGG 8 cut(s) 23, 52, 72, 139, 150, 170, 992, 1288
HgaI GACGC 3 cut(s) 184, 218, 302
HhaI GCGC 1 cut(s) 985
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 2 cut(s) 897, 1389
Hin6I GCGC 1 cut(s) 983
HinP1I GCGC 1 cut(s) 983
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HindIII AAGCTT 2 cut(s) 459, 1274
HpaII CCGG 8 cut(s) 23, 52, 72, 139, 150, 170, 992, 1288
HphI GGTGA 4 cut(s) 30, 637, 1451, 1562
Hpy166II GTNNAC 2 cut(s) 556, 1139
Hpy188III TCNNGA 2 cut(s) 797, 1220
Hpy8I GTNNAC 2 cut(s) 556, 1139
HpyAV CCTTC 3 cut(s) 593, 1659, 1683
HpyCH4III ACNGT 4 cut(s) 158, 454, 921, 1051
HpyCH4IV ACGT 1 cut(s) 1563
HpyF3I CTNAG 6 cut(s) 91, 630, 871, 898, 1476, 1542
HpySE526I ACGT 1 cut(s) 1563
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 2 cut(s) 897, 1389
HspAI GCGC 1 cut(s) 983
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Kzo9I GATC 4 cut(s) 368, 660, 1196, 1254
LguI GCTCTTC 1 cut(s) 1506
LmnI GCTCC 4 cut(s) 127, 792, 1084, 1480
LweI GCATC 7 cut(s) 519, 741, 814, 1010, 1271, 1392, 1479
MaeI CTAG 5 cut(s) 348, 546, 732, 765, 791
MaeII ACGT 1 cut(s) 1563
MaeIII GTNAC 3 cut(s) 134, 152, 625
MalI GATC 4 cut(s) 370, 662, 1198, 1256
MboI GATC 4 cut(s) 368, 660, 1196, 1254
MfeI CAATTG 1 cut(s) 401
MflI RGATCY 1 cut(s) 1196
MhlI GDGCHC 4 cut(s) 102, 367, 491, 789
MlsI TGGCCA 2 cut(s) 1043, 1162
MluNI TGGCCA 2 cut(s) 1043, 1162
MlyI GAGTC 3 cut(s) 559, 602, 1534
MmeI TCCRAC 1 cut(s) 684
Mox20I TGGCCA 2 cut(s) 1043, 1162
Mph1103I ATGCAT 1 cut(s) 1494
MreI CGCCGGCG 1 cut(s) 71
MroNI GCCGGC 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 453
MscI TGGCCA 2 cut(s) 1043, 1162
Msp20I TGGCCA 2 cut(s) 1043, 1162
MspA1I CMGCKG 4 cut(s) 374, 446, 470, 815
MspI CCGG 8 cut(s) 23, 52, 72, 139, 150, 170, 992, 1288
MspR9I CCNGG 2 cut(s) 23, 262
MunI CAATTG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 1297
MvaI CCWGG 1 cut(s) 262
MvnI CGCG 1 cut(s) 212
NaeI GCCGGC 1 cut(s) 73
NciI CCSGG 1 cut(s) 23
NdeI CATATG 1 cut(s) 1494
NdeII GATC 4 cut(s) 368, 660, 1196, 1254
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 2 cut(s) 897, 1389
NmeAIII GCCGAG 3 cut(s) 85, 145, 959
NmuCI GTSAC 1 cut(s) 625
NsiI ATGCAT 1 cut(s) 1494
NspI RCATGY 1 cut(s) 1389
PaeR7I CTCGAG 1 cut(s) 101
PceI AGGCCT 1 cut(s) 1474
PciI ACATGT 1 cut(s) 1385
PciSI GCTCTTC 1 cut(s) 1506
PcsI WCGNNNNNNNCGW 1 cut(s) 364
PctI GAATGC 1 cut(s) 1297
PdiI GCCGGC 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 7 cut(s) 353, 1106, 1376, 1409, 1444, 1532, 1573
PflMI CCANNNNNTGG 1 cut(s) 497
PfoI TCCNGGA 1 cut(s) 260
PleI GAGTC 3 cut(s) 559, 601, 1534
PpsI GAGTC 3 cut(s) 559, 601, 1534
PscI ACATGT 1 cut(s) 1385
Psp124BI GAGCTC 1 cut(s) 789
Psp1406I AACGTT 1 cut(s) 1563
Psp6I CCWGG 1 cut(s) 260
PspEI GGTNACC 2 cut(s) 134, 152
PspFI CCCAGC 1 cut(s) 119
PspGI CCWGG 1 cut(s) 260
PspPI GGNCC 2 cut(s) 147, 1368
PspXI VCTCGAGB 1 cut(s) 101
PstI CTGCAG 1 cut(s) 1009
PstNI CAGNNNCTG 1 cut(s) 1055
PsuI RGATCY 1 cut(s) 1196
PvuII CAGCTG 3 cut(s) 374, 470, 815
RsaI GTAC 5 cut(s) 335, 392, 1138, 1334, 1418
RsaNI GTAC 5 cut(s) 334, 391, 1137, 1333, 1417
SacI GAGCTC 1 cut(s) 789
SapI GCTCTTC 1 cut(s) 1506
Sau3AI GATC 4 cut(s) 368, 660, 1196, 1254
Sau96I GGNCC 2 cut(s) 147, 1368
ScaI AGTACT 2 cut(s) 392, 1334
SchI GAGTC 3 cut(s) 559, 602, 1534
ScrFI CCNGG 2 cut(s) 23, 262
SduI GDGCHC 4 cut(s) 102, 367, 491, 789
SfaNI GCATC 7 cut(s) 519, 741, 814, 1010, 1271, 1392, 1479
SfcI CTRYAG 4 cut(s) 411, 465, 1005, 1336
Sfr274I CTCGAG 1 cut(s) 101
SgrAI CRCCGGYG 1 cut(s) 71
SlaI CTCGAG 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 101, 795
SmoI CTYRAG 2 cut(s) 101, 795
SseBI AGGCCT 1 cut(s) 1474
SsiI CCGC 2 cut(s) 212, 444
SspMI CTAG 5 cut(s) 348, 546, 732, 765, 791
SstI GAGCTC 1 cut(s) 789
StuI AGGCCT 1 cut(s) 1474
StyD4I CCNGG 2 cut(s) 21, 260
TaaI ACNGT 4 cut(s) 158, 454, 921, 1051
TaiI ACGT 1 cut(s) 1566
TaqI TCGA 5 cut(s) 102, 367, 527, 563, 663
TaqII GACCGA 1 cut(s) 76
TatI WGTACW 3 cut(s) 390, 1332, 1416
TfiI GAWTC 7 cut(s) 353, 1106, 1376, 1409, 1444, 1532, 1573
TscAI CASTG 2 cut(s) 879, 1683
TseFI GTSAC 1 cut(s) 625
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 4 cut(s) 665, 729, 1089, 1283
TspGWI ACGGA 1 cut(s) 739
TspRI CASTG 2 cut(s) 879, 1683
Van91I CCANNNNNTGG 1 cut(s) 497
XapI RAATTY 5 cut(s) 10, 314, 676, 877, 906
XceI RCATGY 1 cut(s) 1389
XhoI CTCGAG 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 5 cut(s) 348, 546, 732, 765, 791
ZrmI AGTACT 2 cut(s) 392, 1334
Zsp2I ATGCAT 1 cut(s) 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.