FvH4_1g24210

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017587
Physical Location & Seq
Forward (+)
16087255 .. 16088227
973 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24210.t1

Sequence Viewer

Length: 822 bp
ATGATGGAGTACATTGGAGCCACTGGTGTTTCGGTATCCTTTGACGATGTACCAATCAATGATGAAATCGACTTCAATTTCATACTCGGCTTTGCTATTGATGCAGATGAATCAGGCAACTTCCAAAACGGAAAATTTTCACCATACTGGATACCTGAACTAACCCCAAACTCTGTTGCGGACATAAAAGCATCCCACCCTAATGTAAAAGTCATGGTAAGCCTGTCTGGTTGGAGTATAGGTGAGAAAACCCTACACTGGTACATTCCTGAAAACTCCGAAAAATGGATAAGCAATGCCGTCTCATCTCTCACTTCCATCATCACCACTTACCATCTTGATGGCATTGACGTAGACTACGAAAAGTTCCGCAATACTTCTGCAACTAATGCTAATTTCGCTTACTGCATTGGCGAGCTCATCACCCGTTTGAAAGACCAGGGTGTCATTTCAGTGGCCAGCATTGCACCCTTTTACAGCACAGTTGCGCCTTACATTGCACTCTTTAAGGACTACGGACATGCCATAGACTATGTCAATTACCAGTTCTACACTGACAAGGTTAGGACTCCGAAAGGATACTTGAAGGCATTCGAGAATCGAGCCAACCAATTTGACAAGGCAAAGCTGTTGCCAGCTTATGAAGTGAACGGAAGAGGAATTCAGGGTGATATTTTCTTTGATGCTTTAGACCTATTGGAGAAAAATGGATTCCTGGTTAATGGGGTGATGATCTTCTCAGCTGATGCTTCTTCTGAGAATAAGTATTACTATGAGAAAAAATCACAAGCTTTCTTATTACTCAATTCTAAATCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

274

Amino Acids

30.61

Weight (kDa)

4.88

Isoelectric Point (pI)

13.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 56 - 184 2.5e-11 Glycosyl hydrolases family 18
TIM-barrel_EndoS PF23916 86 - 202 1.8e-07 Endo-beta-N-acetylglucosaminidase EndoS TIM-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017587)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24210
malus_domestica MD02G1278700.v1.1 MD07G1046800.v1.1
prunus_persica Prupe.2G041800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0120651
rosa_laevigata RLG00000018564
rosa_rugosa Rorug02G0230100
rosa_samantha Rh2AG286100 Rh2BG297900 Rh2CG275600 Rh2DG311600
rosa_wichuraiana Rw2G022980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 443
AccI GTMKAC 1 cut(s) 354
AciI CCGC 2 cut(s) 179, 370
AcoI YGGCCR 1 cut(s) 456
AcsI RAATTY 2 cut(s) 134, 660
AfaI GTAC 3 cut(s) 11, 51, 263
AfiI CCNNNNNNNGG 2 cut(s) 258, 285
AgsI TTSAA 3 cut(s) 76, 433, 586
AjnI CCWGG 2 cut(s) 438, 714
AluBI AGCT 5 cut(s) 418, 628, 638, 743, 791
AluI AGCT 5 cut(s) 418, 628, 638, 743, 791
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 1 cut(s) 307
AoxI GGCC 1 cut(s) 456
ApoI RAATTY 2 cut(s) 134, 660
Asp700I GAANNNNTTC 2 cut(s) 136, 590
AspLEI GCGC 1 cut(s) 490
AsuHPI GGTGA 6 cut(s) 132, 254, 316, 415, 680, 739
BalI TGGCCA 1 cut(s) 458
BanII GRGCYC 1 cut(s) 420
Bbv12I GWGCWC 1 cut(s) 420
BccI CCATC 3 cut(s) 326, 335, 342
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 2 cut(s) 440, 716
BciVI GTATCC 3 cut(s) 46, 144, 572
BcoDI GTCTC 1 cut(s) 307
BfuI GTATCC 3 cut(s) 46, 144, 572
Bme1390I CCNGG 2 cut(s) 440, 716
BmiI GGNNCC 1 cut(s) 19
BmrFI CCNGG 2 cut(s) 440, 716
BmsI GCATC 4 cut(s) 91, 200, 673, 736
BsaJI CCNNGG 1 cut(s) 439
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 285
Bse1I ACTGG 4 cut(s) 28, 152, 263, 544
Bse3DI GCAATG 3 cut(s) 301, 462, 495
BseBI CCWGG 2 cut(s) 440, 716
BseDI CCNNGG 1 cut(s) 439
BseGI GGATG 1 cut(s) 191
BseLI CCNNNNNNNGG 2 cut(s) 258, 285
BseMI GCAATG 3 cut(s) 301, 462, 495
BseMII CTCAG 2 cut(s) 747, 753
BseNI ACTGG 4 cut(s) 28, 152, 263, 544
BshFI GGCC 1 cut(s) 458
BsiHKAI GWGCWC 1 cut(s) 420
BslI CCNNNNNNNGG 2 cut(s) 258, 285
BsmAI GTCTC 1 cut(s) 307
BsmBI CGTCTC 1 cut(s) 307
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 420
Bsp143I GATC 1 cut(s) 732
BspACI CCGC 2 cut(s) 179, 370
BspANI GGCC 1 cut(s) 458
BspCNI CTCAG 2 cut(s) 748, 752
BspLI GGNNCC 1 cut(s) 19
BsrDI GCAATG 3 cut(s) 301, 462, 495
BsrI ACTGG 4 cut(s) 28, 152, 263, 544
BssECI CCNNGG 1 cut(s) 439
BssMI GATC 1 cut(s) 732
Bst2UI CCWGG 2 cut(s) 440, 716
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 1 cut(s) 649
BstAPI GCANNNNNTGC 1 cut(s) 389
BstC8I GCNNGC 3 cut(s) 416, 460, 636
BstDEI CTNAG 2 cut(s) 739, 756
BstF5I GGATG 1 cut(s) 191
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 1 cut(s) 735
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 1 cut(s) 732
BstMWI GCNNNNNNNGC 4 cut(s) 101, 389, 398, 464
BstNI CCWGG 2 cut(s) 440, 716
BstNSI RCATGY 1 cut(s) 524
BstSCI CCNGG 2 cut(s) 438, 714
BstXI CCANNNNNNTGG 1 cut(s) 341
BsuI GTATCC 3 cut(s) 46, 144, 572
BsuRI GGCC 1 cut(s) 458
BtsCI GGATG 1 cut(s) 191
BtsIMutI CAGTG 4 cut(s) 21, 256, 459, 552
Cac8I GCNNGC 3 cut(s) 416, 460, 636
CfoI GCGC 1 cut(s) 490
Csp6I GTAC 3 cut(s) 10, 50, 262
CviAII CATG 2 cut(s) 214, 521
CviQI GTAC 3 cut(s) 10, 50, 262
DdeI CTNAG 2 cut(s) 739, 756
DpnI GATC 1 cut(s) 734
DpnII GATC 1 cut(s) 732
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 1 cut(s) 649
EarI CTCTTC 1 cut(s) 649
Ecl136II GAGCTC 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 418
EcoICRI GAGCTC 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 660
EcoRII CCWGG 2 cut(s) 438, 714
EcoT38I GRGCYC 1 cut(s) 420
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 2 cut(s) 217, 524
FatI CATG 2 cut(s) 213, 520
FblI GTMKAC 1 cut(s) 354
FokI GGATG 1 cut(s) 178
FriOI GRGCYC 1 cut(s) 420
GlaI GCGC 1 cut(s) 489
HaeIII GGCC 1 cut(s) 458
HhaI GCGC 1 cut(s) 490
Hin1II CATG 2 cut(s) 217, 524
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HindIII AAGCTT 1 cut(s) 789
HinfI GANTC 4 cut(s) 110, 568, 598, 711
HphI GGTGA 6 cut(s) 132, 254, 316, 415, 680, 739
Hpy166II GTNNAC 2 cut(s) 355, 649
Hpy188I TCNGA 3 cut(s) 280, 573, 757
Hpy188III TCNNGA 3 cut(s) 269, 338, 595
Hpy8I GTNNAC 2 cut(s) 355, 649
HpyAV CCTTC 1 cut(s) 580
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 5 cut(s) 104, 383, 408, 467, 500
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 389, 398, 464
HpyF3I CTNAG 2 cut(s) 739, 756
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 2 cut(s) 217, 524
HspAI GCGC 1 cut(s) 488
Kzo9I GATC 1 cut(s) 732
LmnI GCTCC 1 cut(s) 17
LweI GCATC 4 cut(s) 91, 200, 673, 736
MaeII ACGT 1 cut(s) 351
MalI GATC 1 cut(s) 734
MboI GATC 1 cut(s) 732
MboII GAAGA 3 cut(s) 666, 727, 744
MhlI GDGCHC 1 cut(s) 420
MlsI TGGCCA 1 cut(s) 458
MluCI AATT 7 cut(s) 76, 134, 394, 538, 611, 660, 805
MluNI TGGCCA 1 cut(s) 458
MlyI GAGTC 1 cut(s) 562
MmeI TCCRAC 1 cut(s) 212
MnlI CCTC 1 cut(s) 650
Mox20I TGGCCA 1 cut(s) 458
MroXI GAANNNNTTC 2 cut(s) 136, 590
MscI TGGCCA 1 cut(s) 458
MseI TTAA 2 cut(s) 507, 720
MslI CAYNNNNRTG 3 cut(s) 201, 339, 452
Msp20I TGGCCA 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 743
MspR9I CCNGG 2 cut(s) 440, 716
Mva1269I GAATGC 1 cut(s) 590
MvaI CCWGG 2 cut(s) 440, 716
MwoI GCNNNNNNNGC 4 cut(s) 101, 389, 398, 464
NdeII GATC 1 cut(s) 732
NlaIII CATG 2 cut(s) 217, 524
NlaIV GGNNCC 1 cut(s) 19
NmeAIII GCCGAG 1 cut(s) 66
NspI RCATGY 1 cut(s) 524
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PctI GAATGC 1 cut(s) 590
PdmI GAANNNNTTC 2 cut(s) 136, 590
PfeI GAWTC 3 cut(s) 110, 598, 711
PflFI GACNNNGTC 1 cut(s) 533
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
Psp124BI GAGCTC 1 cut(s) 420
Psp6I CCWGG 2 cut(s) 438, 714
PspGI CCWGG 2 cut(s) 438, 714
PspN4I GGNNCC 1 cut(s) 19
PsyI GACNNNGTC 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 3 cut(s) 11, 51, 263
RsaNI GTAC 3 cut(s) 10, 50, 262
RseI CAYNNNNRTG 3 cut(s) 201, 339, 452
SacI GAGCTC 1 cut(s) 420
SaqAI TTAA 2 cut(s) 507, 720
Sau3AI GATC 1 cut(s) 732
SchI GAGTC 1 cut(s) 562
ScrFI CCNGG 2 cut(s) 440, 716
SduI GDGCHC 1 cut(s) 420
SfaNI GCATC 4 cut(s) 91, 200, 673, 736
SmiMI CAYNNNNRTG 3 cut(s) 201, 339, 452
Sse9I AATT 7 cut(s) 76, 134, 394, 538, 611, 660, 805
SsiI CCGC 2 cut(s) 179, 370
SstI GAGCTC 1 cut(s) 420
StyD4I CCNGG 2 cut(s) 438, 714
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 1 cut(s) 354
TaqI TCGA 3 cut(s) 69, 594, 601
TasI AATT 7 cut(s) 76, 134, 394, 538, 611, 660, 805
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 3 cut(s) 110, 598, 711
Tru1I TTAA 2 cut(s) 507, 720
Tru9I TTAA 2 cut(s) 507, 720
TscAI CASTG 4 cut(s) 28, 263, 459, 559
TspDTI ATGAA 4 cut(s) 70, 78, 123, 657
TspGWI ACGGA 3 cut(s) 144, 531, 666
TspRI CASTG 4 cut(s) 28, 263, 459, 559
Tth111I GACNNNGTC 1 cut(s) 533
XapI RAATTY 2 cut(s) 134, 660
XceI RCATGY 1 cut(s) 524
XmiI GTMKAC 1 cut(s) 354
XmnI GAANNNNTTC 2 cut(s) 136, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.