MD02G1278700.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017587
Physical Location & Seq
Forward (+)
33462712 .. 33463855
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1278700.v1.1.491

Sequence Viewer

Length: 852 bp
ATGTTTCAGCAATTTTGTGACGGGAAGTTGATGATGGAGTACATTGGAGCCACCGGAGTTCCCGTAACATTTGACTCTGTACCGATTGACAATGAGATCGACTTCCATTTCATACTCGGGTTTGCGATTGATGCAGACTCATCAGGCAAACAACAAAATGGGATATTCTCACCATATTGGCAATCTACATTAACCCCACAATCTGTTGCAGCCATCAAGGAAAAAAACCCTAATGTGAAAGTCATGGCAAGCCTCTCAGGTTGGAGCTTAGGTGATAAAGTCCTTCACTGGTACAATCCGACAAACCCCAAAAAATGGGTATCTAACGCTGTCTCGTCGCTCGGTTCCATAATTACCACTTACCATCTTGATGGCATTGACATAGACTACGAAAACTTCCCAAAGCGCGGTAATTCAAGTTCTTTTGCTTTTTGCATCGGCGAGCTCATCACCGGCTTGAAAAAGAAAGGGCTCATTTCCGTTGCTAGTATTGCACCGTTTTATAGTACAGCTGCGCCGTATGTTCAGCTCTTTGATGACTATGCAGATGTTATAGACTATGTCAACCACCAGTTCTACACCGACAAAGTTACTACCCCGAAGGGATATCTGCAAGCCTTTAAGATTCGAGCCGCGCAATTTGGCAAGGAAAAGTTGTTGCCTGCCTATGAAGTAGATGGAAGAGGAATTCAAGGGGATGCCTTTTTTGATGCTTTGACACTGTTGCAGACAAATGGATTTGAAATTAATGGGGTCATGATCTTCTCAGCTGATGCTTCTTTGAAAAACAACTTTCACTATGAGAGAAAATCACAAGCTTTCTTGCTCAACTCTACCAGTAGTACTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

284

Amino Acids

31.37

Weight (kDa)

5.52

Isoelectric Point (pI)

24.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 36 - 193 2.4e-13 Glycosyl hydrolases family 18
TIM-barrel_EndoS PF23916 63 - 209 1.5e-08 Endo-beta-N-acetylglucosaminidase EndoS TIM-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017587)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g24210
malus_domestica MD02G1278700.v1.1 MD07G1046800.v1.1
prunus_persica Prupe.2G041800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0120651
rosa_laevigata RLG00000018564
rosa_rugosa Rorug02G0230100
rosa_samantha Rh2AG286100 Rh2BG297900 Rh2CG275600 Rh2DG311600
rosa_wichuraiana Rw2G022980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 315
AccII CGCG 2 cut(s) 408, 635
AciI CCGC 2 cut(s) 408, 633
AcsI RAATTY 1 cut(s) 687
AfaI GTAC 5 cut(s) 41, 81, 293, 508, 844
AfiI CCNNNNNNNGG 2 cut(s) 315, 407
AgsI TTSAA 5 cut(s) 417, 460, 692, 743, 784
AluBI AGCT 6 cut(s) 267, 445, 512, 529, 770, 818
AluI AGCT 6 cut(s) 267, 445, 512, 529, 770, 818
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 1 cut(s) 337
Ama87I CYCGRG 1 cut(s) 116
ApeKI GCWGC 2 cut(s) 209, 512
ApoI RAATTY 1 cut(s) 687
AseI ATTAAT 1 cut(s) 747
AspLEI GCGC 3 cut(s) 408, 517, 637
AsuHPI GGTGA 3 cut(s) 162, 284, 442
AvaI CYCGRG 1 cut(s) 116
BanII GRGCYC 2 cut(s) 447, 474
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 2 cut(s) 221, 499
BccI CCATC 5 cut(s) 28, 221, 365, 372, 671
BceAI ACGGC 1 cut(s) 502
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 2 cut(s) 486, 850
BisI GCNGC 3 cut(s) 210, 513, 633
BlsI GCNGC 3 cut(s) 211, 514, 634
BmcAI AGTACT 1 cut(s) 844
BmeT110I CYCGRG 1 cut(s) 116
BmiI GGNNCC 2 cut(s) 49, 346
BmsI GCATC 5 cut(s) 121, 444, 688, 700, 763
Bpu10I CCTNAGC 1 cut(s) 268
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 256, 286
Bsc4I CCNNNNNNNGG 2 cut(s) 315, 407
Bse118I RCCGGY 1 cut(s) 452
Bse1I ACTGG 3 cut(s) 293, 571, 837
BseGI GGATG 1 cut(s) 703
BseLI CCNNNNNNNGG 2 cut(s) 315, 407
BseMII CTCAG 2 cut(s) 270, 780
BseNI ACTGG 3 cut(s) 293, 571, 837
BseXI GCAGC 2 cut(s) 221, 499
Bsh1236I CGCG 2 cut(s) 408, 635
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 2 cut(s) 54, 453
BslI CCNNNNNNNGG 2 cut(s) 315, 407
BsmAI GTCTC 1 cut(s) 337
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 447, 474
Bsp143I GATC 2 cut(s) 96, 759
BspACI CCGC 2 cut(s) 408, 633
BspCNI CTCAG 2 cut(s) 269, 779
BspFNI CGCG 2 cut(s) 408, 635
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 2 cut(s) 49, 346
BsrFI RCCGGY 1 cut(s) 452
BsrI ACTGG 3 cut(s) 293, 571, 837
BssAI RCCGGY 1 cut(s) 452
BssMI GATC 2 cut(s) 96, 759
Bst4CI ACNGT 2 cut(s) 498, 723
Bst6I CTCTTC 1 cut(s) 676
BstC8I GCNNGC 4 cut(s) 250, 443, 615, 663
BstDEI CTNAG 3 cut(s) 256, 268, 766
BstF5I GGATG 1 cut(s) 703
BstFNI CGCG 2 cut(s) 408, 635
BstHHI GCGC 3 cut(s) 408, 517, 637
BstKTI GATC 2 cut(s) 99, 762
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 2 cut(s) 96, 759
BstMWI GCNNNNNNNGC 2 cut(s) 131, 491
BstUI CGCG 2 cut(s) 408, 635
BstV1I GCAGC 2 cut(s) 221, 499
BstXI CCANNNNNNTGG 1 cut(s) 371
BtsCI GGATG 1 cut(s) 703
BtsIMutI CAGTG 2 cut(s) 286, 719
Cac8I GCNNGC 4 cut(s) 250, 443, 615, 663
CciI TCATGA 1 cut(s) 756
CfoI GCGC 3 cut(s) 408, 517, 637
Cfr10I RCCGGY 1 cut(s) 452
Csp6I GTAC 5 cut(s) 40, 80, 292, 507, 843
CviAII CATG 2 cut(s) 244, 757
CviQI GTAC 5 cut(s) 40, 80, 292, 507, 843
DdeI CTNAG 3 cut(s) 256, 268, 766
DpnI GATC 2 cut(s) 98, 761
DpnII GATC 2 cut(s) 96, 759
Eam1104I CTCTTC 1 cut(s) 676
EarI CTCTTC 1 cut(s) 676
Ecl136II GAGCTC 1 cut(s) 445
Eco24I GRGCYC 2 cut(s) 447, 474
Eco32I GATATC 1 cut(s) 608
Eco53kI GAGCTC 1 cut(s) 445
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 445
EcoRI GAATTC 1 cut(s) 687
EcoRV GATATC 1 cut(s) 608
EcoT38I GRGCYC 2 cut(s) 447, 474
FaeI CATG 2 cut(s) 247, 760
FatI CATG 2 cut(s) 243, 756
Fnu4HI GCNGC 3 cut(s) 210, 513, 633
FokI GGATG 1 cut(s) 710
FriOI GRGCYC 2 cut(s) 447, 474
Fsp4HI GCNGC 3 cut(s) 210, 513, 633
FspBI CTAG 2 cut(s) 486, 850
GlaI GCGC 3 cut(s) 407, 516, 636
GluI GCNGC 3 cut(s) 210, 513, 633
HapII CCGG 2 cut(s) 54, 453
HhaI GCGC 3 cut(s) 408, 517, 637
Hin1II CATG 2 cut(s) 247, 760
Hin6I GCGC 3 cut(s) 406, 515, 635
HinP1I GCGC 3 cut(s) 406, 515, 635
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 816
HinfI GANTC 3 cut(s) 74, 137, 625
HpaII CCGG 2 cut(s) 54, 453
HphI GGTGA 3 cut(s) 162, 284, 442
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 2 cut(s) 368, 757
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 2 cut(s) 293, 595
HpyCH4III ACNGT 2 cut(s) 498, 723
HpyCH4V TGCA 7 cut(s) 134, 209, 435, 494, 545, 613, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 491
HpyF3I CTNAG 3 cut(s) 256, 268, 766
Hsp92II CATG 2 cut(s) 247, 760
HspAI GCGC 3 cut(s) 406, 515, 635
Kzo9I GATC 2 cut(s) 96, 759
LmnI GCTCC 2 cut(s) 47, 264
LpnPI CCDG 7 cut(s) 67, 129, 243, 274, 466, 584, 675
Lsp1109I GCAGC 2 cut(s) 221, 499
LweI GCATC 5 cut(s) 121, 444, 688, 700, 763
MaeI CTAG 2 cut(s) 486, 850
MaeIII GTNAC 3 cut(s) 17, 64, 589
MalI GATC 2 cut(s) 98, 761
MboI GATC 2 cut(s) 96, 759
MboII GAAGA 2 cut(s) 693, 754
MhlI GDGCHC 2 cut(s) 447, 474
MluCI AATT 6 cut(s) 11, 351, 412, 638, 687, 744
MlyI GAGTC 2 cut(s) 68, 131
MmeI TCCRAC 2 cut(s) 242, 323
MnlI CCTC 2 cut(s) 263, 677
MseI TTAA 3 cut(s) 191, 621, 747
MslI CAYNNNNRTG 1 cut(s) 369
MspA1I CMGCKG 2 cut(s) 512, 770
MspI CCGG 2 cut(s) 54, 453
MvnI CGCG 2 cut(s) 408, 635
MwoI GCNNNNNNNGC 2 cut(s) 131, 491
NdeII GATC 2 cut(s) 96, 759
NlaIII CATG 2 cut(s) 247, 760
NlaIV GGNNCC 2 cut(s) 49, 346
NmuCI GTSAC 1 cut(s) 17
PagI TCATGA 1 cut(s) 756
PfeI GAWTC 1 cut(s) 625
PflFI GACNNNGTC 1 cut(s) 560
PflMI CCANNNNNTGG 1 cut(s) 315
PkrI GCNGC 3 cut(s) 211, 514, 634
PleI GAGTC 2 cut(s) 68, 131
PpsI GAGTC 2 cut(s) 68, 131
PshBI ATTAAT 1 cut(s) 747
Psp124BI GAGCTC 1 cut(s) 447
PspN4I GGNNCC 2 cut(s) 49, 346
PsrI GAACNNNNNNTAC 2 cut(s) 403, 435
PsyI GACNNNGTC 1 cut(s) 560
PvuII CAGCTG 2 cut(s) 512, 770
RsaI GTAC 5 cut(s) 41, 81, 293, 508, 844
RsaNI GTAC 5 cut(s) 40, 80, 292, 507, 843
RseI CAYNNNNRTG 1 cut(s) 369
SacI GAGCTC 1 cut(s) 447
SaqAI TTAA 3 cut(s) 191, 621, 747
SatI GCNGC 3 cut(s) 210, 513, 633
Sau3AI GATC 2 cut(s) 96, 759
ScaI AGTACT 1 cut(s) 844
SchI GAGTC 2 cut(s) 68, 131
SduI GDGCHC 2 cut(s) 447, 474
SetI ASST 8 cut(s) 262, 269, 274, 447, 514, 531, 772, 820
SfaNI GCATC 5 cut(s) 121, 444, 688, 700, 763
SmiMI CAYNNNNRTG 1 cut(s) 369
Sse9I AATT 6 cut(s) 11, 351, 412, 638, 687, 744
SsiI CCGC 2 cut(s) 408, 633
SspMI CTAG 2 cut(s) 486, 850
SstI GAGCTC 1 cut(s) 447
TaaI ACNGT 2 cut(s) 498, 723
TaqI TCGA 2 cut(s) 99, 628
TasI AATT 6 cut(s) 11, 351, 412, 638, 687, 744
TatI WGTACW 3 cut(s) 39, 506, 842
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 3 cut(s) 191, 621, 747
Tru9I TTAA 3 cut(s) 191, 621, 747
TscAI CASTG 2 cut(s) 293, 726
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 2 cut(s) 209, 512
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 2 cut(s) 100, 684
TspGWI ACGGA 1 cut(s) 469
TspRI CASTG 2 cut(s) 293, 726
Tth111I GACNNNGTC 1 cut(s) 560
Van91I CCANNNNNTGG 1 cut(s) 315
VspI ATTAAT 1 cut(s) 747
XapI RAATTY 1 cut(s) 687
XspI CTAG 2 cut(s) 486, 850
ZrmI AGTACT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.