FvH4_1g28990

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014551
Physical Location & Seq
Reverse (-)
21426896 .. 21432725
5830 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28990.t3

Sequence Viewer

Length: 1707 bp
ATGGCGGAGTCAGATTGCGAAATCGATGGGAACCAGCTGATCGCAAAGTCGCTGGCCCGTTTCGGCGTGGATTGCATGTTCGGCGTGGTGGGAATCCCTGTGACCTCCCTAGCCAACCGCGCAGTCTCGCTCGGTGTCCGATTTCTCGCCTTCCATAACGAGCAGTCCGCCGGTTATGCAGCCTCTGCCTACGGTTACCTAACCGGCAGGCCCGGCGTCCTCCTCACCGTCTCGGGTCCTGGCTGCGTCCACGGCCTGGCGGGCCTCGCCAACGCCATGGCCAACGGGTGGCCCATGGTGATGATCTCCGGCTCCTGTGATCAGAATGATTTCGGCCGTGGCGATTTTCAGGAGCTCGATCAGATCGCCGCCGTGAAACCCTTTTCCAAGTTCTCCGTGAAAGCCAAGGCTATCAAGGAGATACCCGACTGCGTCTTCCAGGCTCTTGCTCATGCCGGTTCGGGCCGGCCCGGCGGGTGTTACTTGGATTTCCCGTCGGATGTGTTGCACCAGACAATTTCCGATTCGGAGGCCGAGGGCCTACTGGGCAGTGCTGCCGAGAGAATCCGGGAAGAAAAAGTGGTTAGCGTTGAGAATTCGCAGATTGAGGAAGCGGCTTCACTGCTTCGACATGCGGAGAGGCCCCTGATCGTGTTTGGTAAAGGAGCCGCCTTTTCTCGAGCTGAGAGCGCTCTGACCAAGCTAGTGGAGACCACCGGAATCCCCTTCCTGCCTACTCCTATGGGGAAGGGTCTGTTGCCCGATACTCACGACCTGGCGGCAACTGCGGCGAGGTCCTTGGCCATCGGCAAATGTGACGTGGCGCTTGTTGTTGGCGCACGCCTTAACTGGCTCCTGCACTTTGGTGACCCACCCAAGTGGTCCAAGGACGTCAAGTTCATTTTGGTTGATGTTTGCCGGGAAGAGATTGAGCTCAGGAAACCTCATCTGGGTTTGGTTGGTGATGCCAAGTTGGTCTTGGAAAAATTGAATAAGCAGATTAAAGATGACCCTTTTTGTTTGGGGAGGTCTCATCCATGGGTGGAAGCAATTTCCCGCAAGGCTAAGGAGAATGTTTCCAAGTTGGAGGCCCAACTGGCTAAACAAGTTGTCCCTTTTAATTTCCTGACACCAATGAAGATTATCAGAGATGCAATTTGCGCACTGGGTAGCCCTGCTCCTATATTGGTTTCTGAAGGGGCAAACACCATGGATGTTGGCCGAGCGGTACTTGTTCAGACTGAACCCAGGACCAGATTGGATGCAGGGACTTGGGGGACCATGGGGGTCGGTCTTGGTTACTGCATTGCAGCTGCTGTGGCTTCTCCTGACCGGCTTGTGGTTGCGGTGGAAGGAGATTCTGGGTTTGGGTTTAGTGCAATGGAAGTTGAGACATTGGTTCGTTACCAGTTGCCTGTTGTTGTTATTGTTTTCAACAATGGTGGTGTATATGGTGGTGACCGAAGAAGCCCAGATGAAATCAATGGGCCTTACAAAGATGACCCTGCTCCCACTTCTTTTGTCCCTAGTGCCGCTTATCACATAGTGATTGAAGCTTTTGGGGGCAAAGGTTATCTTGCTTGCTCCCCGGAGGAACTCAGTTCTGCCCTTGCGGAATCCTTTGCAGCAAGAAAACCAGCTGTTATAAATGTAATAATTGATCCTTACGCTGGTTCAGAAAGTGGAAGACTTCAACACAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

569

Amino Acids

60.39

Weight (kDa)

5.76

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 9 - 123 1.5e-31 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 202 - 330 4e-34 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 400 - 465 2.5e-10 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17380
fragaria_vesca FvH4_1g28990
malus_domestica MD00G1054500.v1.1 MD09G1258100.v1.1
prunus_persica Prupe.3G150000_v2.0.a1
pyrus_communis pycom09g17290 pycom17g25510
rosa_chinensis RchiOBHm_Chr3g0494041
rosa_laevigata RLG00000022686
rosa_multiflora Rmu_sc0003412.1_g000008
rosa_roxburghii Rroxscaffold_6G00391310
rosa_rugosa Rorug03G0263700
rosa_samantha Rh3AG313400 Rh3BG349500 Rh3CG346300 Rh3DG348800
rosa_wichuraiana Rw3G027430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1646
AatII GACGTC 1 cut(s) 894
Acc16I TGCGCA 1 cut(s) 1162
AccB7I CCANNNNNTGG 2 cut(s) 256, 288
AccBSI CCGCTC 1 cut(s) 1226
AccII CGCG 1 cut(s) 120
AclWI GGATC 1 cut(s) 1655
AcoI YGGCCR 4 cut(s) 279, 334, 801, 1219
AcsI RAATTY 1 cut(s) 595
AcuI CTGAAG 1 cut(s) 1215
AcyI GRCGYC 2 cut(s) 216, 891
AdeI CACNNNGTG 1 cut(s) 1546
AfaI GTAC 1 cut(s) 1230
AfeI AGCGCT 1 cut(s) 691
AfiI CCNNNNNNNGG 5 cut(s) 256, 288, 950, 1339, 1670
AgsI TTSAA 4 cut(s) 991, 1435, 1553, 1694
AjiI CACGTC 1 cut(s) 820
AjnI CCWGG 5 cut(s) 238, 255, 438, 774, 1247
AleI CACNNNNGTG 2 cut(s) 864, 877
AloI GAACNNNNNNTCC 4 cut(s) 62, 94, 881, 913
AluBI AGCT 8 cut(s) 37, 355, 683, 703, 934, 1313, 1556, 1640
AluI AGCT 8 cut(s) 37, 355, 683, 703, 934, 1313, 1556, 1640
Alw21I GWGCWC 2 cut(s) 357, 936
Alw26I GTCTC 5 cut(s) 130, 235, 704, 1035, 1385
AlwI GGATC 1 cut(s) 1655
AlwNI CAGNNNCTG 2 cut(s) 185, 1316
Ama87I CYCGRG 2 cut(s) 232, 678
Aor51HI AGCGCT 1 cut(s) 691
ApeKI GCWGC 6 cut(s) 179, 243, 554, 1310, 1313, 1625
ApoI RAATTY 1 cut(s) 595
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 5 cut(s) 122, 692, 826, 839, 1163
AsuC2I CCSGG 5 cut(s) 213, 471, 569, 920, 1589
AsuHPI GGTGA 5 cut(s) 217, 310, 878, 974, 1469
AvaI CYCGRG 2 cut(s) 232, 678
AvaII GGWCC 5 cut(s) 236, 795, 882, 1251, 1278
BalI TGGCCA 2 cut(s) 281, 803
BanII GRGCYC 2 cut(s) 357, 936
BbsI GAAGAC 2 cut(s) 427, 1693
Bbv12I GWGCWC 2 cut(s) 357, 936
BbvI GCAGC 6 cut(s) 191, 230, 541, 1300, 1322, 1637
BccI CCATC 2 cut(s) 20, 812
BceAI ACGGC 3 cut(s) 268, 321, 356
BciT130I CCWGG 5 cut(s) 240, 257, 440, 776, 1249
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 5 cut(s) 213, 471, 569, 920, 1589
BcoDI GTCTC 5 cut(s) 130, 235, 704, 1035, 1385
BfaI CTAG 3 cut(s) 110, 704, 1527
BfoI RGCGCY 2 cut(s) 693, 827
BglI GCCNNNNNGGC 4 cut(s) 261, 471, 546, 1097
Bme18I GGWCC 5 cut(s) 236, 795, 882, 1251, 1278
BmeT110I CYCGRG 2 cut(s) 232, 678
BmgBI CACGTC 1 cut(s) 820
BmiI GGNNCC 7 cut(s) 32, 237, 313, 644, 667, 854, 1279
BmrI ACTGGG 2 cut(s) 554, 1175
BmsI GCATC 3 cut(s) 955, 1141, 1252
BmuI ACTGGG 2 cut(s) 554, 1175
BpiI GAAGAC 2 cut(s) 427, 1693
Bpu10I CCTNAGC 2 cut(s) 935, 1065
BpuMI CCSGG 5 cut(s) 213, 471, 569, 920, 1589
Bsa29I ATCGAT 1 cut(s) 24
BsaHI GRCGYC 2 cut(s) 216, 891
BsaI GGTCTC 2 cut(s) 704, 1035
BsaWI WCCGGW 1 cut(s) 716
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 288, 950, 1339, 1670
Bse118I RCCGGY 5 cut(s) 170, 203, 455, 465, 1332
Bse1I ACTGG 5 cut(s) 549, 854, 1101, 1170, 1408
Bse3DI GCAATG 2 cut(s) 1305, 1386
BseBI CCWGG 5 cut(s) 240, 257, 440, 776, 1249
BseCI ATCGAT 1 cut(s) 24
BseGI GGATG 4 cut(s) 505, 1033, 1219, 1267
BseLI CCNNNNNNNGG 5 cut(s) 256, 288, 950, 1339, 1670
BseMI GCAATG 2 cut(s) 1305, 1386
BseMII CTCAG 3 cut(s) 675, 949, 1612
BseNI ACTGG 5 cut(s) 549, 854, 1101, 1170, 1408
BseRI GAGGAG 1 cut(s) 212
BseX3I CGGCCG 1 cut(s) 334
BseXI GCAGC 6 cut(s) 191, 230, 541, 1300, 1322, 1637
BsgI GTGCAG 1 cut(s) 842
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 337
BshVI ATCGAT 1 cut(s) 24
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 2 cut(s) 357, 936
BsiHKCI CYCGRG 2 cut(s) 232, 678
BslFI GGGAC 4 cut(s) 1097, 1282, 1291, 1508
BslI CCNNNNNNNGG 5 cut(s) 256, 288, 950, 1339, 1670
BsmAI GTCTC 5 cut(s) 130, 235, 704, 1035, 1385
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 4 cut(s) 1097, 1282, 1291, 1508
Bso31I GGTCTC 2 cut(s) 704, 1035
BsoBI CYCGRG 2 cut(s) 232, 678
Bsp1286I GDGCHC 2 cut(s) 357, 936
Bsp143I GATC 7 cut(s) 39, 303, 319, 358, 363, 648, 1660
Bsp19I CCATGG 5 cut(s) 276, 294, 1037, 1209, 1281
BspCNI CTCAG 3 cut(s) 676, 948, 1611
BspDI ATCGAT 1 cut(s) 24
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 7 cut(s) 32, 237, 313, 644, 667, 854, 1279
BspPI GGATC 1 cut(s) 1655
BspTNI GGTCTC 2 cut(s) 704, 1035
BsrBI CCGCTC 1 cut(s) 1226
BsrDI GCAATG 2 cut(s) 1305, 1386
BsrFI RCCGGY 5 cut(s) 170, 203, 455, 465, 1332
BsrI ACTGG 5 cut(s) 549, 854, 1101, 1170, 1408
BssAI RCCGGY 5 cut(s) 170, 203, 455, 465, 1332
BssMI GATC 7 cut(s) 39, 303, 319, 358, 363, 648, 1660
BssNI GRCGYC 2 cut(s) 216, 891
BssT1I CCWWGG 8 cut(s) 276, 294, 405, 798, 885, 1037, 1209, 1281
Bst2UI CCWGG 5 cut(s) 240, 257, 440, 776, 1249
Bst4CI ACNGT 2 cut(s) 194, 229
Bst6I CTCTTC 1 cut(s) 918
BstACI GRCGYC 2 cut(s) 216, 891
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 6 cut(s) 54, 209, 262, 467, 841, 1582
BstDEI CTNAG 4 cut(s) 684, 935, 1065, 1598
BstDSI CCRYGG 7 cut(s) 250, 276, 294, 337, 1037, 1209, 1281
BstEII GGTNACC 3 cut(s) 194, 866, 1457
BstF5I GGATG 4 cut(s) 505, 1033, 1219, 1267
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 2 cut(s) 693, 827
BstHHI GCGC 5 cut(s) 122, 692, 826, 839, 1163
BstKTI GATC 7 cut(s) 42, 306, 322, 361, 366, 651, 1663
BstMAI GTCTC 5 cut(s) 130, 235, 704, 1035, 1385
BstMBI GATC 7 cut(s) 39, 303, 319, 358, 363, 648, 1660
BstMCI CGRYCG 1 cut(s) 337
BstNI CCWGG 5 cut(s) 240, 257, 440, 776, 1249
BstNSI RCATGY 2 cut(s) 79, 635
BstPI GGTNACC 3 cut(s) 194, 866, 1457
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 6 cut(s) 191, 230, 541, 1300, 1322, 1637
BstV2I GAAGAC 2 cut(s) 427, 1693
BstXI CCANNNNNNTGG 3 cut(s) 277, 706, 879
BstZI CGGCCG 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 24
BsuTUI ATCGAT 1 cut(s) 24
BtgI CCRYGG 7 cut(s) 250, 276, 294, 337, 1037, 1209, 1281
BtrI CACGTC 1 cut(s) 820
BtsCI GGATG 4 cut(s) 505, 1033, 1219, 1267
BtsI GCAGTG 2 cut(s) 556, 620
BtsIMutI CAGTG 3 cut(s) 556, 620, 1163
Cac8I GCNNGC 6 cut(s) 54, 209, 262, 467, 841, 1582
CaiI CAGNNNCTG 2 cut(s) 185, 1316
CfoI GCGC 5 cut(s) 122, 692, 826, 839, 1163
Cfr10I RCCGGY 5 cut(s) 170, 203, 455, 465, 1332
ClaI ATCGAT 1 cut(s) 24
CseI GACGC 3 cut(s) 205, 235, 421
Csp6I GTAC 1 cut(s) 1229
CspCI CAANNNNNGTGG 4 cut(s) 1423, 1458, 1501, 1536
CviAII CATG 8 cut(s) 76, 277, 295, 452, 632, 1038, 1210, 1282
CviQI GTAC 1 cut(s) 1229
DdeI CTNAG 4 cut(s) 684, 935, 1065, 1598
DpnI GATC 7 cut(s) 41, 305, 321, 360, 365, 650, 1662
DpnII GATC 7 cut(s) 39, 303, 319, 358, 363, 648, 1660
DraIII CACNNNGTG 1 cut(s) 1546
EaeI YGGCCR 4 cut(s) 279, 334, 801, 1219
EagI CGGCCG 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
EciI GGCGGA 2 cut(s) 20, 157
Ecl136II GAGCTC 2 cut(s) 355, 934
EclXI CGGCCG 1 cut(s) 334
Eco130I CCWWGG 8 cut(s) 276, 294, 405, 798, 885, 1037, 1209, 1281
Eco24I GRGCYC 2 cut(s) 357, 936
Eco31I GGTCTC 2 cut(s) 704, 1035
Eco47I GGWCC 5 cut(s) 236, 795, 882, 1251, 1278
Eco47III AGCGCT 1 cut(s) 691
Eco52I CGGCCG 1 cut(s) 334
Eco53kI GAGCTC 2 cut(s) 355, 934
Eco57I CTGAAG 1 cut(s) 1215
Eco88I CYCGRG 2 cut(s) 232, 678
Eco91I GGTNACC 3 cut(s) 194, 866, 1457
EcoICRI GAGCTC 2 cut(s) 355, 934
EcoO109I RGGNCCY 4 cut(s) 236, 538, 642, 795
EcoO65I GGTNACC 3 cut(s) 194, 866, 1457
EcoRI GAATTC 1 cut(s) 595
EcoRII CCWGG 5 cut(s) 238, 255, 438, 774, 1247
EcoT14I CCWWGG 8 cut(s) 276, 294, 405, 798, 885, 1037, 1209, 1281
EcoT38I GRGCYC 2 cut(s) 357, 936
ErhI CCWWGG 8 cut(s) 276, 294, 405, 798, 885, 1037, 1209, 1281
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 8 cut(s) 79, 280, 298, 455, 635, 1041, 1213, 1285
FalI AAGNNNNNCTT 4 cut(s) 962, 994, 1560, 1592
FaqI GGGAC 4 cut(s) 1097, 1282, 1291, 1508
FatI CATG 8 cut(s) 75, 276, 294, 451, 631, 1037, 1209, 1281
FauI CCCGC 3 cut(s) 253, 467, 1064
FbaI TGATCA 1 cut(s) 319
FokI GGATG 4 cut(s) 512, 1020, 1226, 1274
FriOI GRGCYC 2 cut(s) 357, 936
FseI GGCCGGCC 1 cut(s) 469
FspBI CTAG 3 cut(s) 110, 704, 1527
FspI TGCGCA 1 cut(s) 1162
GlaI GCGC 5 cut(s) 121, 691, 825, 838, 1162
HaeII RGCGCY 2 cut(s) 693, 827
HgaI GACGC 3 cut(s) 205, 235, 421
HhaI GCGC 5 cut(s) 122, 692, 826, 839, 1163
Hin1I GRCGYC 2 cut(s) 216, 891
Hin1II CATG 8 cut(s) 79, 280, 298, 455, 635, 1041, 1213, 1285
Hin6I GCGC 5 cut(s) 120, 690, 824, 837, 1161
HinP1I GCGC 5 cut(s) 120, 690, 824, 837, 1161
HindIII AAGCTT 1 cut(s) 1554
HinfI GANTC 7 cut(s) 8, 93, 524, 564, 720, 1358, 1616
HphI GGTGA 5 cut(s) 217, 310, 878, 974, 1469
Hpy166II GTNNAC 1 cut(s) 250
Hpy188III TCNNGA 6 cut(s) 350, 678, 770, 937, 1126, 1328
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 1 cut(s) 499
HpyAV CCTTC 5 cut(s) 160, 736, 742, 1190, 1346
HpyCH4III ACNGT 2 cut(s) 194, 229
HpyCH4IV ACGT 2 cut(s) 819, 891
HpyF3I CTNAG 4 cut(s) 684, 935, 1065, 1598
HpySE526I ACGT 2 cut(s) 819, 891
Hsp92I GRCGYC 2 cut(s) 216, 891
Hsp92II CATG 8 cut(s) 79, 280, 298, 455, 635, 1041, 1213, 1285
HspAI GCGC 5 cut(s) 120, 690, 824, 837, 1161
KroI GCCGGC 1 cut(s) 465
KroNI GCCGGC 1 cut(s) 467
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 7 cut(s) 39, 303, 319, 358, 363, 648, 1660
LmnI GCTCC 7 cut(s) 317, 352, 665, 858, 1183, 1513, 1589
Lsp1109I GCAGC 6 cut(s) 191, 230, 541, 1300, 1322, 1637
LweI GCATC 3 cut(s) 955, 1141, 1252
MaeI CTAG 3 cut(s) 110, 704, 1527
MaeII ACGT 2 cut(s) 819, 891
MaeIII GTNAC 8 cut(s) 100, 194, 479, 815, 866, 1298, 1403, 1457
MalI GATC 7 cut(s) 41, 305, 321, 360, 365, 650, 1662
MbiI CCGCTC 1 cut(s) 1226
MboI GATC 7 cut(s) 39, 303, 319, 358, 363, 648, 1660
MboII GAAGA 6 cut(s) 427, 584, 935, 1150, 1476, 1698
MhlI GDGCHC 2 cut(s) 357, 936
MlsI TGGCCA 2 cut(s) 281, 803
MluCI AATT 7 cut(s) 516, 595, 986, 1050, 1120, 1155, 1656
MluNI TGGCCA 2 cut(s) 281, 803
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 2 cut(s) 477, 1065
Mox20I TGGCCA 2 cut(s) 281, 803
MroNI GCCGGC 1 cut(s) 465
MroXI GAANNNNTTC 1 cut(s) 329
MscI TGGCCA 2 cut(s) 281, 803
MseI TTAA 3 cut(s) 846, 1002, 1119
MslI CAYNNNNRTG 3 cut(s) 299, 864, 877
Msp20I TGGCCA 2 cut(s) 281, 803
MspA1I CMGCKG 3 cut(s) 37, 1313, 1640
MvaI CCWGG 5 cut(s) 240, 257, 440, 776, 1249
MvnI CGCG 1 cut(s) 120
NaeI GCCGGC 1 cut(s) 467
NciI CCSGG 5 cut(s) 213, 471, 569, 920, 1589
NcoI CCATGG 5 cut(s) 276, 294, 1037, 1209, 1281
NdeII GATC 7 cut(s) 39, 303, 319, 358, 363, 648, 1660
NgoMIV GCCGGC 1 cut(s) 465
NlaIII CATG 8 cut(s) 79, 280, 298, 455, 635, 1041, 1213, 1285
NlaIV GGNNCC 7 cut(s) 32, 237, 313, 644, 667, 854, 1279
NmeAIII GCCGAG 3 cut(s) 559, 583, 1247
NmuCI GTSAC 4 cut(s) 100, 815, 866, 1457
NsbI TGCGCA 1 cut(s) 1162
NspI RCATGY 2 cut(s) 79, 635
OliI CACNNNNGTG 2 cut(s) 864, 877
PaeR7I CTCGAG 1 cut(s) 678
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PdiI GCCGGC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 6 cut(s) 93, 524, 564, 720, 1358, 1616
PflFI GACNNNGTC 1 cut(s) 431
PflMI CCANNNNNTGG 2 cut(s) 256, 288
PfoI TCCNGGA 1 cut(s) 567
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PpuMI RGGWCCY 2 cut(s) 236, 795
PsiI TTATAA 1 cut(s) 1646
Psp124BI GAGCTC 2 cut(s) 357, 936
Psp5II RGGWCCY 2 cut(s) 236, 795
Psp6I CCWGG 5 cut(s) 238, 255, 438, 774, 1247
PspEI GGTNACC 3 cut(s) 194, 866, 1457
PspGI CCWGG 5 cut(s) 238, 255, 438, 774, 1247
PspN4I GGNNCC 7 cut(s) 32, 237, 313, 644, 667, 854, 1279
PspPPI RGGWCCY 2 cut(s) 236, 795
PstNI CAGNNNCTG 2 cut(s) 185, 1316
PsyI GACNNNGTC 1 cut(s) 431
PvuII CAGCTG 3 cut(s) 37, 1313, 1640
RigI GGCCGGCC 1 cut(s) 469
RsaI GTAC 1 cut(s) 1230
RsaNI GTAC 1 cut(s) 1229
RseI CAYNNNNRTG 3 cut(s) 299, 864, 877
SacI GAGCTC 2 cut(s) 357, 936
SaqAI TTAA 3 cut(s) 846, 1002, 1119
Sau3AI GATC 7 cut(s) 39, 303, 319, 358, 363, 648, 1660
SchI GAGTC 1 cut(s) 17
SduI GDGCHC 2 cut(s) 357, 936
SfaNI GCATC 3 cut(s) 955, 1141, 1252
SfiI GGCCNNNNNGGCC 1 cut(s) 261
Sfr274I CTCGAG 1 cut(s) 678
SinI GGWCC 5 cut(s) 236, 795, 882, 1251, 1278
SlaI CTCGAG 1 cut(s) 678
SmiMI CAYNNNNRTG 3 cut(s) 299, 864, 877
SmlI CTYRAG 1 cut(s) 678
SmoI CTYRAG 1 cut(s) 678
Sse9I AATT 7 cut(s) 516, 595, 986, 1050, 1120, 1155, 1656
SspMI CTAG 3 cut(s) 110, 704, 1527
SstI GAGCTC 2 cut(s) 357, 936
StyI CCWWGG 8 cut(s) 276, 294, 405, 798, 885, 1037, 1209, 1281
TaaI ACNGT 2 cut(s) 194, 229
TaiI ACGT 2 cut(s) 822, 894
TaqI TCGA 4 cut(s) 24, 357, 628, 679
TaqII GACCGA 2 cut(s) 1280, 1476
TasI AATT 7 cut(s) 516, 595, 986, 1050, 1120, 1155, 1656
TauI GCSGC 6 cut(s) 371, 617, 671, 782, 791, 1535
TfiI GAWTC 6 cut(s) 93, 524, 564, 720, 1358, 1616
Tru1I TTAA 3 cut(s) 846, 1002, 1119
Tru9I TTAA 3 cut(s) 846, 1002, 1119
TscAI CASTG 3 cut(s) 556, 627, 1170
TseFI GTSAC 4 cut(s) 100, 815, 866, 1457
TseI GCWGC 6 cut(s) 179, 243, 554, 1310, 1313, 1625
Tsp45I GTSAC 4 cut(s) 100, 815, 866, 1457
TspDTI ATGAA 3 cut(s) 889, 1151, 1491
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 3 cut(s) 556, 627, 1170
Tth111I GACNNNGTC 1 cut(s) 431
Van91I CCANNNNNTGG 2 cut(s) 256, 288
VpaK11BI GGWCC 5 cut(s) 236, 795, 882, 1251, 1278
XapI RAATTY 1 cut(s) 595
XceI RCATGY 2 cut(s) 79, 635
XcmI CCANNNNNNNNNTGG 2 cut(s) 976, 1255
XhoI CTCGAG 1 cut(s) 678
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 3 cut(s) 110, 704, 1527
ZraI GACGTC 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.