Rroxscaffold_6G00391310

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014551
Physical Location & Seq
Reverse (-)
9170672 .. 9175377
4706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391310.1

Sequence Viewer

Length: 1863 bp
ATGGGCCATGGCGGAGACGTCATTGTTCCACCGAACGAAAAAAGGAGCCGCCAATTTGGTTCCTCTTATCCTTGGAGTCTCTACCCACTCCTCATCGTCGCCCTGGTATTTATTACCCAGGGCATAAGCGTTATTCAAAATCCAACTCGCACTCTCTTCAGGAAAGTCGAAGCTCAACTATCAGAGCAGCATAGCATAGCATACATGGCGGAGTCAGACGCCCACAATTTACAAACCGTCCTCCTTGATGGCAACCAGCTGATTGCCAAGTCCCTGGCCCGTTTCGGCGTAGACCGCATGTTTGGCGTGCAGTCTGCCGGCTATGCAGCCTCCGCCTACGGTTACCTAACGGGCCGGCCCGGCGTCCTCCTCACCGTTTCAGGCCCTGGCTGCGTCCACGGGCTGGCGGGCCTCGCCAACGCCATGGCCAATGGGTGGCCCATGGTCATGATCTCCGGCTCCTGTGATCAGAAAGATTTCGGCCGCGGCGATTTCCAGGAGCTCGATCAGATCGCAGCCGTGAAACCCTTTTCCAAGTTCGCTGTTAAAGCCAAGGATATTAACGAGATACCCGAATGCGTGTTGCAGGCTCTTGCTCATGCCGGTTCGGCCCGACCCGGCGGTTGTTATTTGGATTTCCCGTCGGATGTTCTACACCAGACTATTTCCGATTCGGAGGCTGAGGGCCTACTGGCCGGTGCTGCCGAGAGATTCCGAAAATTATCGGAAGAGGTGGTTTTCAATGTGCAGAATTCGCAGATTGAGGAAGCGGCTTCACTGCTTCGACACGCGGAGAGGCCTCTGATTGTGTTTGGTAAAGGAGCGGCGTTTGCTCGAGCTGAGAGTGCTCTGACGAAGCTAGTTGAGACCACCGGAATCCCATTCCTGCCTACTCCTATGGGAAAGGGATTGTTGCCCGATACCCACGACCTGGCGGCAACCGCGGCGAGGTCACTGGCCATCGGCAAATGTGACGTTGCGCTTGTGGTTGGCGCAAGGCTTAACTGGCTCTTGCACTTTGGTGAGCCACCCAAGTGGTCCAAGGACGTCAAGTTCATTTTGGTTGATGTTTGCCAGGAAGAGATTGAGCTGAGAAAACCTCATCTGGGTTTGGTTGGTGATGCCAAATTGGTTTTGGAAAAATTGAATAAGCAGATTAAAGATGACCCCTTTTGTTTGGGGAGGTCTCATCCATGGGTCGAAGCAATTTCCCGCAAGGCCAAGGAGAACGTTTTGAAAATGGAGGCCCAATTGGCTAAACAAGTTGTGCCCTTTAATTTCTTGACACCAATGAAAATTATTAGAGATGCAATTTTGGGACTGGGTACCCCTGCTCCTATACTGGTTTCTGAGGGGGCAAACACCATGGATGTTGGCCGAGCTGTATTGGTTCAGACTGAACCGAGGACCAGATTGGATGCAGGGACTTGGGGGACGATGGGGGTGGGTCTTGGTTACTGCATTGCAGCTGCTGTGGCTTCTCCTGACCGGCTTGTGGTTGCGGTGGAAGGAGATTCTGGATTTGGGTTCAGTGCAATGGAAGTTGAGACATTGGTTCGGTACCAGTTGCCTGTTGTTGTGATTGTCTTCAACAATGGTGGTGTATACGGTGGTGACCGAAGAAGCCCAGATGAAATAAATGGGCCTTACAAAGATGACCCTGCTCCCACTTCTTTCGTCCCTAGTGCCGCTTATCACACAATGATTGAAGCTTTTGGAGGCAAAGGTTATCTTGCTGGGACACCTGAGGAACTCAAGTCTGCCATTGCGGAATCCTTTGCAGCAAGAAAACCAGCTGTTATAAATGTAATAATTGATCCTTATGCTGGTTCAGAAAGTGGGAGACTTCAACATAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

620

Amino Acids

66.53

Weight (kDa)

6.15

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 104 - 172 1.8e-19 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 254 - 382 5.4e-34 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 452 - 603 2.5e-26 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17380
fragaria_vesca FvH4_1g28990
malus_domestica MD00G1054500.v1.1 MD09G1258100.v1.1
prunus_persica Prupe.3G150000_v2.0.a1
pyrus_communis pycom09g17290 pycom17g25510
rosa_chinensis RchiOBHm_Chr3g0494041
rosa_laevigata RLG00000022686
rosa_multiflora Rmu_sc0003412.1_g000008
rosa_roxburghii Rroxscaffold_6G00391310
rosa_rugosa Rorug03G0263700
rosa_samantha Rh3AG313400 Rh3BG349500 Rh3CG346300 Rh3DG348800
rosa_wichuraiana Rw3G027430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1802
AatII GACGTC 2 cut(s) 21, 1050
Acc65I GGTACC 2 cut(s) 1325, 1560
AccB1I GGYRCC 2 cut(s) 1325, 1560
AccB7I CCANNNNNTGG 3 cut(s) 403, 435, 931
AccBSI CCGCTC 1 cut(s) 824
AccI GTMKAC 2 cut(s) 291, 1605
AccII CGCG 3 cut(s) 486, 791, 944
AclI AACGTT 1 cut(s) 1230
AclWI GGATC 1 cut(s) 1811
AcoI YGGCCR 5 cut(s) 426, 481, 693, 957, 1375
AcsI RAATTY 1 cut(s) 751
AcuI CTGAAG 1 cut(s) 142
AcyI GRCGYC 4 cut(s) 18, 219, 363, 1047
AfaI GTAC 2 cut(s) 1327, 1562
AfiI CCNNNNNNNGG 7 cut(s) 403, 435, 931, 948, 1106, 1495, 1826
AgsI TTSAA 7 cut(s) 137, 742, 1147, 1237, 1591, 1709, 1850
AjnI CCWGG 7 cut(s) 102, 117, 273, 385, 495, 930, 1074
AleI CACNNNNGTG 2 cut(s) 1020, 1033
AloI GAACNNNNNNTCC 2 cut(s) 1037, 1069
Alw21I GWGCWC 2 cut(s) 504, 850
Alw26I GTCTC 6 cut(s) 9, 83, 860, 1191, 1541, 1837
AlwI GGATC 1 cut(s) 1811
AlwNI CAGNNNCTG 2 cut(s) 386, 1472
Ama87I CYCGRG 1 cut(s) 834
ApeKI GCWGC 8 cut(s) 187, 326, 390, 515, 701, 1466, 1469, 1781
ApoI RAATTY 1 cut(s) 751
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp700I GAANNNNTTC 1 cut(s) 476
Asp718I GGTACC 2 cut(s) 1325, 1560
AspLEI GCGC 2 cut(s) 982, 995
AsuC2I CCSGG 2 cut(s) 360, 618
AsuHPI GGTGA 4 cut(s) 364, 1034, 1130, 1625
AvaI CYCGRG 1 cut(s) 834
AvaII GGWCC 2 cut(s) 1038, 1407
AxyI CCTNAGG 1 cut(s) 1746
BaeGI GKGCMC 1 cut(s) 1272
BalI TGGCCA 2 cut(s) 428, 959
BanI GGYRCC 2 cut(s) 1325, 1560
BanII GRGCYC 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 1579
Bbv12I GWGCWC 2 cut(s) 504, 850
BbvCI CCTCAGC 1 cut(s) 681
BbvI GCAGC 8 cut(s) 199, 338, 377, 527, 688, 1456, 1478, 1793
BccI CCATC 3 cut(s) 242, 968, 1432
BceAI ACGGC 1 cut(s) 503
BciT130I CCWGG 7 cut(s) 104, 119, 275, 387, 497, 932, 1076
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 2 cut(s) 360, 618
BcoDI GTCTC 6 cut(s) 9, 83, 860, 1191, 1541, 1837
BfaI CTAG 2 cut(s) 860, 1683
BglI GCCNNNNNGGC 3 cut(s) 360, 608, 1253
Bme1390I CCNGG 9 cut(s) 104, 119, 275, 360, 387, 497, 618, 932, 1076
Bme18I GGWCC 2 cut(s) 1038, 1407
BmeT110I CYCGRG 1 cut(s) 834
BmiI GGNNCC 5 cut(s) 47, 61, 460, 1327, 1562
BmrFI CCNGG 9 cut(s) 104, 119, 275, 360, 387, 497, 618, 932, 1076
BmrI ACTGGG 1 cut(s) 1331
BmsI GCATC 3 cut(s) 1111, 1297, 1408
BmuI ACTGGG 1 cut(s) 1331
BpiI GAAGAC 1 cut(s) 1579
BplI GAGNNNNNCTC 2 cut(s) 1084, 1116
Bpu10I CCTNAGC 1 cut(s) 681
BpuEI CTTGAG 1 cut(s) 1739
BpuMI CCSGG 2 cut(s) 360, 618
BsaHI GRCGYC 4 cut(s) 18, 219, 363, 1047
BsaI GGTCTC 2 cut(s) 860, 1191
BsaWI WCCGGW 1 cut(s) 872
BsaXI ACNNNNNCTCC 2 cut(s) 1318, 1348
Bsc4I CCNNNNNNNGG 7 cut(s) 403, 435, 931, 948, 1106, 1495, 1826
Bse118I RCCGGY 5 cut(s) 317, 354, 602, 695, 1488
Bse1I ACTGG 6 cut(s) 696, 960, 1010, 1326, 1347, 1564
Bse21I CCTNAGG 1 cut(s) 1746
Bse3DI GCAATG 3 cut(s) 1461, 1542, 1764
BseBI CCWGG 7 cut(s) 104, 119, 275, 387, 497, 932, 1076
BseGI GGATG 4 cut(s) 652, 1189, 1375, 1423
BseLI CCNNNNNNNGG 7 cut(s) 403, 435, 931, 948, 1106, 1495, 1826
BseMI GCAATG 3 cut(s) 1461, 1542, 1764
BseMII CTCAG 5 cut(s) 672, 831, 1082, 1341, 1737
BseNI ACTGG 6 cut(s) 696, 960, 1010, 1326, 1347, 1564
BseRI GAGGAG 2 cut(s) 80, 359
BseSI GKGCMC 1 cut(s) 1272
BseX3I CGGCCG 1 cut(s) 481
BseXI GCAGC 8 cut(s) 199, 338, 377, 527, 688, 1456, 1478, 1793
BseYI CCCAGC 1 cut(s) 1736
BsgI GTGCAG 2 cut(s) 329, 767
Bsh1236I CGCG 3 cut(s) 486, 791, 944
Bsh1285I CGRYCG 1 cut(s) 484
BshNI GGYRCC 2 cut(s) 1325, 1560
BsiEI CGRYCG 1 cut(s) 484
BsiHKAI GWGCWC 2 cut(s) 504, 850
BsiHKCI CYCGRG 1 cut(s) 834
BsiSI CCGG 9 cut(s) 318, 355, 360, 456, 603, 618, 696, 873, 1489
BslFI GGGAC 6 cut(s) 256, 1332, 1438, 1447, 1664, 1753
BslI CCNNNNNNNGG 7 cut(s) 403, 435, 931, 948, 1106, 1495, 1826
BsmAI GTCTC 6 cut(s) 9, 83, 860, 1191, 1541, 1837
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 6 cut(s) 256, 1332, 1438, 1447, 1664, 1753
BsmI GAATGC 1 cut(s) 581
Bso31I GGTCTC 2 cut(s) 860, 1191
BsoBI CYCGRG 1 cut(s) 834
Bsp1286I GDGCHC 3 cut(s) 504, 850, 1272
Bsp143I GATC 5 cut(s) 450, 466, 505, 510, 1816
Bsp19I CCATGG 5 cut(s) 7, 423, 441, 1193, 1365
BspCNI CTCAG 5 cut(s) 673, 832, 1083, 1342, 1738
BspFNI CGCG 3 cut(s) 486, 791, 944
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 5 cut(s) 47, 61, 460, 1327, 1562
BspPI GGATC 1 cut(s) 1811
BspT107I GGYRCC 2 cut(s) 1325, 1560
BspTNI GGTCTC 2 cut(s) 860, 1191
BsrBI CCGCTC 1 cut(s) 824
BsrDI GCAATG 3 cut(s) 1461, 1542, 1764
BsrFI RCCGGY 5 cut(s) 317, 354, 602, 695, 1488
BsrI ACTGG 6 cut(s) 696, 960, 1010, 1326, 1347, 1564
BssAI RCCGGY 5 cut(s) 317, 354, 602, 695, 1488
BssMI GATC 5 cut(s) 450, 466, 505, 510, 1816
BssNAI GTATAC 1 cut(s) 1606
BssNI GRCGYC 4 cut(s) 18, 219, 363, 1047
BssT1I CCWWGG 9 cut(s) 7, 71, 423, 441, 552, 1041, 1193, 1221, 1365
Bst1107I GTATAC 1 cut(s) 1606
Bst2UI CCWGG 7 cut(s) 104, 119, 275, 387, 497, 932, 1076
Bst4CI ACNGT 4 cut(s) 238, 341, 376, 1610
Bst6I CTCTTC 3 cut(s) 161, 723, 1074
BstACI GRCGYC 4 cut(s) 18, 219, 363, 1047
BstC8I GCNNGC 6 cut(s) 308, 319, 356, 405, 409, 588
BstDEI CTNAG 5 cut(s) 681, 840, 1091, 1350, 1746
BstDSI CCRYGG 8 cut(s) 7, 397, 423, 441, 484, 942, 1193, 1365
BstEII GGTNACC 2 cut(s) 341, 1613
BstF5I GGATG 4 cut(s) 652, 1189, 1375, 1423
BstFNI CGCG 3 cut(s) 486, 791, 944
BstHHI GCGC 2 cut(s) 982, 995
BstKTI GATC 5 cut(s) 453, 469, 508, 513, 1819
BstMAI GTCTC 6 cut(s) 9, 83, 860, 1191, 1541, 1837
BstMBI GATC 5 cut(s) 450, 466, 505, 510, 1816
BstMCI CGRYCG 1 cut(s) 484
BstNI CCWGG 7 cut(s) 104, 119, 275, 387, 497, 932, 1076
BstNSI RCATGY 1 cut(s) 301
BstPI GGTNACC 2 cut(s) 341, 1613
BstSCI CCNGG 9 cut(s) 102, 117, 273, 358, 385, 495, 616, 930, 1074
BstSLI GKGCMC 1 cut(s) 1272
BstUI CGCG 3 cut(s) 486, 791, 944
BstV1I GCAGC 8 cut(s) 199, 338, 377, 527, 688, 1456, 1478, 1793
BstV2I GAAGAC 1 cut(s) 1579
BstXI CCANNNNNNTGG 3 cut(s) 274, 424, 1035
BstZ17I GTATAC 1 cut(s) 1606
BstZI CGGCCG 1 cut(s) 481
Bsu36I CCTNAGG 1 cut(s) 1746
BtgI CCRYGG 8 cut(s) 7, 397, 423, 441, 484, 942, 1193, 1365
BtsCI GGATG 4 cut(s) 652, 1189, 1375, 1423
BtsI GCAGTG 1 cut(s) 776
BtsIMutI CAGTG 3 cut(s) 776, 953, 1537
Cac8I GCNNGC 6 cut(s) 308, 319, 356, 405, 409, 588
CaiI CAGNNNCTG 2 cut(s) 386, 1472
CciI TCATGA 1 cut(s) 447
CfoI GCGC 2 cut(s) 982, 995
Cfr10I RCCGGY 5 cut(s) 317, 354, 602, 695, 1488
Cfr42I CCGCGG 2 cut(s) 487, 945
CseI GACGC 3 cut(s) 227, 352, 382
Csp6I GTAC 2 cut(s) 1326, 1561
CspCI CAANNNNNGTGG 2 cut(s) 1579, 1614
CviAII CATG 9 cut(s) 8, 205, 298, 424, 442, 448, 599, 1194, 1366
CviQI GTAC 2 cut(s) 1326, 1561
DdeI CTNAG 5 cut(s) 681, 840, 1091, 1350, 1746
DpnI GATC 5 cut(s) 452, 468, 507, 512, 1818
DpnII GATC 5 cut(s) 450, 466, 505, 510, 1816
EaeI YGGCCR 5 cut(s) 426, 481, 693, 957, 1375
EagI CGGCCG 1 cut(s) 481
Eam1104I CTCTTC 3 cut(s) 161, 723, 1074
EarI CTCTTC 3 cut(s) 161, 723, 1074
EciI GGCGGA 3 cut(s) 27, 224, 322
Ecl136II GAGCTC 1 cut(s) 502
EclXI CGGCCG 1 cut(s) 481
Eco130I CCWWGG 9 cut(s) 7, 71, 423, 441, 552, 1041, 1193, 1221, 1365
Eco147I AGGCCT 1 cut(s) 799
Eco24I GRGCYC 1 cut(s) 504
Eco31I GGTCTC 2 cut(s) 860, 1191
Eco47I GGWCC 2 cut(s) 1038, 1407
Eco52I CGGCCG 1 cut(s) 481
Eco53kI GAGCTC 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 142
Eco81I CCTNAGG 1 cut(s) 1746
Eco88I CYCGRG 1 cut(s) 834
Eco91I GGTNACC 2 cut(s) 341, 1613
EcoICRI GAGCTC 1 cut(s) 502
EcoO109I RGGNCCY 2 cut(s) 383, 685
EcoO65I GGTNACC 2 cut(s) 341, 1613
EcoRI GAATTC 1 cut(s) 751
EcoRII CCWGG 7 cut(s) 102, 117, 273, 385, 495, 930, 1074
EcoT14I CCWWGG 9 cut(s) 7, 71, 423, 441, 552, 1041, 1193, 1221, 1365
EcoT38I GRGCYC 1 cut(s) 504
ErhI CCWWGG 9 cut(s) 7, 71, 423, 441, 552, 1041, 1193, 1221, 1365
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 9 cut(s) 11, 208, 301, 427, 445, 451, 602, 1197, 1369
FalI AAGNNNNNCTT 2 cut(s) 1716, 1748
FaqI GGGAC 6 cut(s) 256, 1332, 1438, 1447, 1664, 1753
FatI CATG 9 cut(s) 7, 204, 297, 423, 441, 447, 598, 1193, 1365
FauI CCCGC 2 cut(s) 400, 1220
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 2 cut(s) 291, 1605
FokI GGATG 4 cut(s) 659, 1176, 1382, 1430
FriOI GRGCYC 1 cut(s) 504
FseI GGCCGGCC 1 cut(s) 358
FspBI CTAG 2 cut(s) 860, 1683
GlaI GCGC 2 cut(s) 981, 994
GsaI CCCAGC 1 cut(s) 1740
HapII CCGG 9 cut(s) 318, 355, 360, 456, 603, 618, 696, 873, 1489
HgaI GACGC 3 cut(s) 227, 352, 382
HhaI GCGC 2 cut(s) 982, 995
Hin1I GRCGYC 4 cut(s) 18, 219, 363, 1047
Hin1II CATG 9 cut(s) 11, 208, 301, 427, 445, 451, 602, 1197, 1369
Hin6I GCGC 2 cut(s) 980, 993
HinP1I GCGC 2 cut(s) 980, 993
HindIII AAGCTT 1 cut(s) 1710
HinfI GANTC 7 cut(s) 76, 212, 671, 711, 876, 1514, 1772
HpaII CCGG 9 cut(s) 318, 355, 360, 456, 603, 618, 696, 873, 1489
HphI GGTGA 4 cut(s) 364, 1034, 1130, 1625
Hpy166II GTNNAC 3 cut(s) 292, 397, 1606
Hpy188III TCNNGA 5 cut(s) 160, 448, 1282, 1484, 1518
Hpy8I GTNNAC 3 cut(s) 292, 397, 1606
Hpy99I CGWCG 2 cut(s) 101, 646
HpyAV CCTTC 1 cut(s) 1502
HpyCH4III ACNGT 4 cut(s) 238, 341, 376, 1610
HpyCH4IV ACGT 4 cut(s) 18, 975, 1047, 1230
HpyF3I CTNAG 5 cut(s) 681, 840, 1091, 1350, 1746
HpySE526I ACGT 4 cut(s) 18, 975, 1047, 1230
Hsp92I GRCGYC 4 cut(s) 18, 219, 363, 1047
Hsp92II CATG 9 cut(s) 11, 208, 301, 427, 445, 451, 602, 1197, 1369
HspAI GCGC 2 cut(s) 980, 993
KpnI GGTACC 2 cut(s) 1329, 1564
KroI GCCGGC 2 cut(s) 317, 354
KroNI GCCGGC 2 cut(s) 319, 356
Ksp22I TGATCA 1 cut(s) 466
KspI CCGCGG 2 cut(s) 487, 945
Kzo9I GATC 5 cut(s) 450, 466, 505, 510, 1816
LmnI GCTCC 6 cut(s) 45, 464, 499, 821, 1339, 1669
Lsp1109I GCAGC 8 cut(s) 199, 338, 377, 527, 688, 1456, 1478, 1793
LweI GCATC 3 cut(s) 1111, 1297, 1408
MaeI CTAG 2 cut(s) 860, 1683
MaeII ACGT 4 cut(s) 18, 975, 1047, 1230
MaeIII GTNAC 5 cut(s) 341, 951, 971, 1454, 1613
MalI GATC 5 cut(s) 452, 468, 507, 512, 1818
MbiI CCGCTC 1 cut(s) 824
MboI GATC 5 cut(s) 450, 466, 505, 510, 1816
MboII GAAGA 5 cut(s) 148, 740, 1091, 1579, 1632
MfeI CAATTG 1 cut(s) 1250
MhlI GDGCHC 3 cut(s) 504, 850, 1272
MlsI TGGCCA 2 cut(s) 428, 959
MluNI TGGCCA 2 cut(s) 428, 959
MlyI GAGTC 2 cut(s) 85, 221
MmeI TCCRAC 2 cut(s) 167, 624
Mox20I TGGCCA 2 cut(s) 428, 959
MroNI GCCGGC 2 cut(s) 317, 354
MroXI GAANNNNTTC 1 cut(s) 476
MscI TGGCCA 2 cut(s) 428, 959
MseI TTAA 5 cut(s) 546, 561, 1002, 1158, 1275
MslI CAYNNNNRTG 3 cut(s) 446, 1020, 1033
Msp20I TGGCCA 2 cut(s) 428, 959
MspA1I CMGCKG 5 cut(s) 259, 486, 944, 1469, 1796
MspI CCGG 9 cut(s) 318, 355, 360, 456, 603, 618, 696, 873, 1489
MspR9I CCNGG 9 cut(s) 104, 119, 275, 360, 387, 497, 618, 932, 1076
MunI CAATTG 1 cut(s) 1250
Mva1269I GAATGC 1 cut(s) 581
MvaI CCWGG 7 cut(s) 104, 119, 275, 387, 497, 932, 1076
MvnI CGCG 3 cut(s) 486, 791, 944
NaeI GCCGGC 2 cut(s) 319, 356
NciI CCSGG 2 cut(s) 360, 618
NcoI CCATGG 5 cut(s) 7, 423, 441, 1193, 1365
NdeII GATC 5 cut(s) 450, 466, 505, 510, 1816
NgoMIV GCCGGC 2 cut(s) 317, 354
NlaIII CATG 9 cut(s) 11, 208, 301, 427, 445, 451, 602, 1197, 1369
NlaIV GGNNCC 5 cut(s) 47, 61, 460, 1327, 1562
NmeAIII GCCGAG 2 cut(s) 730, 1403
NmuCI GTSAC 3 cut(s) 951, 971, 1613
NspI RCATGY 1 cut(s) 301
OliI CACNNNNGTG 2 cut(s) 1020, 1033
PaeR7I CTCGAG 1 cut(s) 834
PagI TCATGA 1 cut(s) 447
PasI CCCWGGG 1 cut(s) 118
PceI AGGCCT 1 cut(s) 799
PcsI WCGNNNNNNNCGW 2 cut(s) 486, 570
PctI GAATGC 1 cut(s) 581
PdiI GCCGGC 2 cut(s) 319, 356
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 5 cut(s) 671, 711, 876, 1514, 1772
PflMI CCANNNNNTGG 3 cut(s) 403, 435, 931
PfoI TCCNGGA 1 cut(s) 495
PleI GAGTC 2 cut(s) 84, 220
PpsI GAGTC 2 cut(s) 84, 220
PsiI TTATAA 1 cut(s) 1802
Psp124BI GAGCTC 1 cut(s) 504
Psp1406I AACGTT 1 cut(s) 1230
Psp6I CCWGG 7 cut(s) 102, 117, 273, 385, 495, 930, 1074
PspEI GGTNACC 2 cut(s) 341, 1613
PspFI CCCAGC 1 cut(s) 1736
PspGI CCWGG 7 cut(s) 102, 117, 273, 385, 495, 930, 1074
PspN4I GGNNCC 5 cut(s) 47, 61, 460, 1327, 1562
PspXI VCTCGAGB 1 cut(s) 834
PstNI CAGNNNCTG 2 cut(s) 386, 1472
PvuII CAGCTG 3 cut(s) 259, 1469, 1796
RigI GGCCGGCC 1 cut(s) 358
RsaI GTAC 2 cut(s) 1327, 1562
RsaNI GTAC 2 cut(s) 1326, 1561
RseI CAYNNNNRTG 3 cut(s) 446, 1020, 1033
SacI GAGCTC 1 cut(s) 504
SacII CCGCGG 2 cut(s) 487, 945
SaqAI TTAA 5 cut(s) 546, 561, 1002, 1158, 1275
Sau3AI GATC 5 cut(s) 450, 466, 505, 510, 1816
SchI GAGTC 2 cut(s) 85, 221
ScrFI CCNGG 9 cut(s) 104, 119, 275, 360, 387, 497, 618, 932, 1076
SduI GDGCHC 3 cut(s) 504, 850, 1272
SfaNI GCATC 3 cut(s) 1111, 1297, 1408
Sfr274I CTCGAG 1 cut(s) 834
Sfr303I CCGCGG 2 cut(s) 487, 945
SgrBI CCGCGG 2 cut(s) 487, 945
SinI GGWCC 2 cut(s) 1038, 1407
SlaI CTCGAG 1 cut(s) 834
SmiMI CAYNNNNRTG 3 cut(s) 446, 1020, 1033
SmlI CTYRAG 2 cut(s) 834, 1754
SmoI CTYRAG 2 cut(s) 834, 1754
SseBI AGGCCT 1 cut(s) 799
SspMI CTAG 2 cut(s) 860, 1683
SstI GAGCTC 1 cut(s) 504
StuI AGGCCT 1 cut(s) 799
StyD4I CCNGG 9 cut(s) 102, 117, 273, 358, 385, 495, 616, 930, 1074
StyI CCWWGG 9 cut(s) 7, 71, 423, 441, 552, 1041, 1193, 1221, 1365
TaaI ACNGT 4 cut(s) 238, 341, 376, 1610
TaiI ACGT 4 cut(s) 21, 978, 1050, 1233
TaqI TCGA 5 cut(s) 168, 504, 784, 835, 1200
TaqII GACCGA 1 cut(s) 1632
TauI GCSGC 8 cut(s) 51, 486, 489, 773, 827, 938, 947, 1691
TfiI GAWTC 5 cut(s) 671, 711, 876, 1514, 1772
Tru1I TTAA 5 cut(s) 546, 561, 1002, 1158, 1275
Tru9I TTAA 5 cut(s) 546, 561, 1002, 1158, 1275
TscAI CASTG 3 cut(s) 783, 960, 1537
TseFI GTSAC 3 cut(s) 951, 971, 1613
TseI GCWGC 8 cut(s) 187, 326, 390, 515, 701, 1466, 1469, 1781
Tsp45I GTSAC 3 cut(s) 951, 971, 1613
TspDTI ATGAA 3 cut(s) 1045, 1307, 1647
TspRI CASTG 3 cut(s) 783, 960, 1537
Van91I CCANNNNNTGG 3 cut(s) 403, 435, 931
VpaK11BI GGWCC 2 cut(s) 1038, 1407
XapI RAATTY 1 cut(s) 751
XceI RCATGY 1 cut(s) 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 1132
XhoI CTCGAG 1 cut(s) 834
XmiI GTMKAC 2 cut(s) 291, 1605
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 2 cut(s) 860, 1683
ZraI GACGTC 2 cut(s) 19, 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.