MD00G1054500.v1.1

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014551
Physical Location & Seq
Forward (+)
10127705 .. 10136863
9159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1054500.v1.1.491

Sequence Viewer

Length: 1713 bp
ATGGGTGAATCGACCACAACCGCAGTCGATGGCAACCAGCTGATCGCCAAGGCCCTTGCCCGGTTCGGCGTCGACCGCATGTTCGGAGTCGTGGGAATTCCTGTCACCTCGCTCGCGAACCGCGCGGTTTCGCTCGGAATCCGGTTTATCGCGTTCCACAACGAGCAGTCGGCGGGTTACGCCGCCTCTGCCTACGGGTATCTAACGGGTCGACCCGGAGTTCTCCTCACGGTCTCGGGTCCCGGATGCGTCCACGGGCTCGCGGGTCTTTCCAACGCCATGGCCAATGCTTGGCCCATGGTAATGATCTCGGGTTCGTGCGACCAGAAAGATTTCGGCCGTGGAGATTTCCAGGAGCTCGATCAGATCGCCGCCGTCGAACCCTTTTCCAAATTCTCCGCCAAAGCTAAATCAATCAAAGAAATACCGGATTGCGTGTTTCAAGCTCTTGCAAAAGCCGGTTCGGGTCGACCCGGAGGGTGCTATTTGGATTTTCCGTCGGATGTACTGCACCAGACAATTTCTGAGGCGGAGGCTGAGAGCTTATTGGCCACCGCCGCCGAGAAATTCGGAGAGTCGGAGAAGGTTCTGTGTGTGCAGAATTCGCAGATTGAGGAGGCCGTTTCGCTGCTCCGGCACGCGGAGAGGCCGTTGATTGTTTTTGGTAAAGGGGCTGCATTTGCTCGAGCGGAGAACGAGTTGGGTAAGCTGGTGGAGAGGACGGGAATCCCATTTTTGCCCACTCCAATGGGGAAGGGGCTGTTGCCGGACACTCATGAGCTGGCGGCGACCGCAGCAAGGTCTCTGGCGATCGGGAAATGTGACGTGGCGCTTGTCGTTGGCGCAAGGCTTAACTGGCTTTTGCACTTTGGTGAGCCGCCCAAGTGGTCCAAGGATGTGAAGTTCATTTTGGTTGATGTTAGTAAGGAAGAGATTGAGCTGCGAAAACCACATTTGGGTTTGGTTGGAGATGCAAAGTTGGTATTGGAGAAGATCAATTTGGGGATCAAGGATGACCCCTTTTGTTTGGGGAAGTCTCATCCGTGGGTTGCAGCGATTTCGAGTAAGGTGAAGGACAATGTTTCGAAAATGGAGGCTCAGTTGGCTAAGGAAGTTGTACCATTTAATTTCTTGACGCCAATGAAGATTATTAGAGATGCAATTGCAGGGTTGGGGAGCCCTGCTCCTATATTGGTTTCCGAGGGGGCTAACACTATGGATGTGGGTCGGTCTGTGTTGGTTCAGACCGAGCCGAGGACCAGGTTGGATGCAGGGACTTGGGGAACAATGGGGGTTGGATTGGGTTACTGCATTGCGGGTGCGGTAGCTTCTCCTGACCGGCTTGTTGTTGCAGTGGAAGGGGATTCAGGATTTGGGTTCAGTGCAATGGAAGTTGAGACATTGGTTCGATACCAGTTACCTGTTGTTGTGATAGTCTTTAACAATGGTGGTGTATATGGTGGCGATCGAAGAAGCCCGGATGAAATTAGTGGGCCTTACAAGGATGACCCTGCTCCCACTTCCTTTGTCCCAAGTGCGGGTTATCACACTCTGATTGAAGCTTTTGGAGGCAAAGGCTATCTTGTTGGGACACCTGAAGAACTCAAGTCCGCCCTTTCAGAATCCTTCTCGGCACAAAAACCAGCTGTTATAAATGTAATTATCGATCCTTATGCTGGTGCAGAAAGTGGGAGGCTTCAACACAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

571

Amino Acids

60.37

Weight (kDa)

5.8

Isoelectric Point (pI)

29.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 11 - 124 1.7e-31 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 204 - 332 8.1e-34 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 405 - 553 2.2e-25 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17380
fragaria_vesca FvH4_1g28990
malus_domestica MD00G1054500.v1.1 MD09G1258100.v1.1
prunus_persica Prupe.3G150000_v2.0.a1
pyrus_communis pycom09g17290 pycom17g25510
rosa_chinensis RchiOBHm_Chr3g0494041
rosa_laevigata RLG00000022686
rosa_multiflora Rmu_sc0003412.1_g000008
rosa_roxburghii Rroxscaffold_6G00391310
rosa_rugosa Rorug03G0263700
rosa_samantha Rh3AG313400 Rh3BG349500 Rh3CG346300 Rh3DG348800
rosa_wichuraiana Rw3G027430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1652
AccB7I CCANNNNNTGG 1 cut(s) 291
AccBSI CCGCTC 1 cut(s) 689
AccI GTMKAC 3 cut(s) 72, 211, 469
AccII CGCG 6 cut(s) 116, 123, 125, 152, 263, 641
AclWI GGATC 2 cut(s) 1013, 1661
AcoI YGGCCR 3 cut(s) 282, 337, 549
AcsI RAATTY 4 cut(s) 96, 392, 566, 601
AcuI CTGAAG 1 cut(s) 1617
AcyI GRCGYC 2 cut(s) 69, 1136
AfaI GTAC 2 cut(s) 507, 1119
AfiI CCNNNNNNNGG 9 cut(s) 60, 291, 640, 798, 956, 1254, 1537, 1538, 1676
AgsI TTSAA 3 cut(s) 443, 1559, 1700
AjiI CACGTC 1 cut(s) 826
AjnI CCWGG 2 cut(s) 351, 1259
AjuI GAANNNNNNNTTGG 4 cut(s) 893, 925, 983, 1015
AleI CACNNNNGTG 1 cut(s) 870
AloI GAACNNNNNNTCC 2 cut(s) 887, 919
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 4 cut(s) 238, 807, 1041, 1391
AlwI GGATC 2 cut(s) 1013, 1661
Ama87I CYCGRG 3 cut(s) 235, 310, 684
AoxI GGCC 8 cut(s) 51, 282, 293, 337, 549, 618, 647, 1493
ApeKI GCWGC 5 cut(s) 628, 674, 794, 940, 1052
ApoI RAATTY 4 cut(s) 96, 392, 566, 601
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 3 cut(s) 125, 832, 845
AspS9I GGNCC 6 cut(s) 52, 239, 294, 888, 1257, 1493
AsuC2I CCSGG 5 cut(s) 61, 216, 243, 474, 1478
AsuHPI GGTGA 4 cut(s) 17, 97, 884, 1081
AsuII TTCGAA 1 cut(s) 1085
AvaI CYCGRG 3 cut(s) 235, 310, 684
AvaII GGWCC 3 cut(s) 239, 888, 1257
BalI TGGCCA 2 cut(s) 284, 551
BanII GRGCYC 3 cut(s) 261, 360, 1181
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 5 cut(s) 615, 661, 806, 927, 1064
BccI CCATC 1 cut(s) 23
BceAI ACGGC 4 cut(s) 324, 359, 605, 634
BcgI CGANNNNNNTGC 4 cut(s) 1041, 1075, 1655, 1689
BciT130I CCWGG 2 cut(s) 353, 1261
BcnI CCSGG 5 cut(s) 61, 216, 243, 474, 1478
BcoDI GTCTC 4 cut(s) 238, 807, 1041, 1391
BfoI RGCGCY 1 cut(s) 833
Bme1390I CCNGG 7 cut(s) 61, 216, 243, 353, 474, 1261, 1478
Bme18I GGWCC 3 cut(s) 239, 888, 1257
BmeT110I CYCGRG 3 cut(s) 235, 310, 684
BmgBI CACGTC 1 cut(s) 826
BmgT120I GGNCC 6 cut(s) 52, 239, 294, 888, 1257, 1493
BmiI GGNNCC 3 cut(s) 240, 241, 1178
BmrFI CCNGG 7 cut(s) 61, 216, 243, 353, 474, 1261, 1478
BmsI GCATC 4 cut(s) 236, 961, 1147, 1258
BplI GAGNNNNNCTC 4 cut(s) 210, 242, 1168, 1200
Bpu10I CCTNAGC 1 cut(s) 1107
Bpu14I TTCGAA 1 cut(s) 1085
BpuEI CTTGAG 1 cut(s) 1589
BpuMI CCSGG 5 cut(s) 61, 216, 243, 474, 1478
Bsa29I ATCGAT 1 cut(s) 1665
BsaHI GRCGYC 2 cut(s) 69, 1136
BsaI GGTCTC 2 cut(s) 238, 807
BsaJI CCNNGG 9 cut(s) 48, 253, 279, 297, 340, 891, 1043, 1200, 1253
BsaWI WCCGGW 2 cut(s) 141, 427
BsaXI ACNNNNNCTCC 6 cut(s) 635, 665, 683, 713, 1085, 1115
Bsc4I CCNNNNNNNGG 9 cut(s) 60, 291, 640, 798, 956, 1254, 1537, 1538, 1676
Bse118I RCCGGY 2 cut(s) 458, 1338
Bse1I ACTGG 2 cut(s) 860, 1414
Bse3DI GCAATG 2 cut(s) 1311, 1392
BseBI CCWGG 2 cut(s) 353, 1261
BseCI ATCGAT 1 cut(s) 1665
BseDI CCNNGG 9 cut(s) 48, 253, 279, 297, 340, 891, 1043, 1200, 1253
BseGI GGATG 9 cut(s) 251, 508, 901, 1018, 1039, 1225, 1273, 1486, 1510
BseLI CCNNNNNNNGG 9 cut(s) 60, 291, 640, 798, 956, 1254, 1537, 1538, 1676
BseMI GCAATG 2 cut(s) 1311, 1392
BseMII CTCAG 3 cut(s) 516, 528, 1112
BseNI ACTGG 2 cut(s) 860, 1414
BseRI GAGGAG 2 cut(s) 215, 629
BseX3I CGGCCG 1 cut(s) 337
BseXI GCAGC 5 cut(s) 615, 661, 806, 927, 1064
BsgI GTGCAG 3 cut(s) 494, 617, 1701
Bsh1236I CGCG 6 cut(s) 116, 123, 125, 152, 263, 641
Bsh1285I CGRYCG 5 cut(s) 76, 340, 792, 813, 1468
BshFI GGCC 8 cut(s) 53, 284, 295, 339, 551, 620, 649, 1495
BshVI ATCGAT 1 cut(s) 1665
BsiEI CGRYCG 5 cut(s) 76, 340, 792, 813, 1468
BsiHKAI GWGCWC 1 cut(s) 360
BsiHKCI CYCGRG 3 cut(s) 235, 310, 684
BslFI GGGAC 4 cut(s) 225, 1288, 1514, 1603
BslI CCNNNNNNNGG 9 cut(s) 60, 291, 640, 798, 956, 1254, 1537, 1538, 1676
BsmAI GTCTC 4 cut(s) 238, 807, 1041, 1391
BsmFI GGGAC 4 cut(s) 225, 1288, 1514, 1603
BsnI GGCC 8 cut(s) 53, 284, 295, 339, 551, 620, 649, 1495
Bso31I GGTCTC 2 cut(s) 238, 807
BsoBI CYCGRG 3 cut(s) 235, 310, 684
Bsp119I TTCGAA 1 cut(s) 1085
Bsp1286I GDGCHC 3 cut(s) 261, 360, 1181
Bsp143I GATC 9 cut(s) 42, 306, 361, 366, 810, 993, 1005, 1465, 1666
Bsp19I CCATGG 2 cut(s) 279, 297
Bsp68I TCGCGA 1 cut(s) 116
BspANI GGCC 8 cut(s) 53, 284, 295, 339, 551, 620, 649, 1495
BspCNI CTCAG 3 cut(s) 517, 529, 1111
BspDI ATCGAT 1 cut(s) 1665
BspFNI CGCG 6 cut(s) 116, 123, 125, 152, 263, 641
BspHI TCATGA 1 cut(s) 775
BspLI GGNNCC 3 cut(s) 240, 241, 1178
BspPI GGATC 2 cut(s) 1013, 1661
BspT104I TTCGAA 1 cut(s) 1085
BspTNI GGTCTC 2 cut(s) 238, 807
BsrBI CCGCTC 1 cut(s) 689
BsrDI GCAATG 2 cut(s) 1311, 1392
BsrFI RCCGGY 2 cut(s) 458, 1338
BsrI ACTGG 2 cut(s) 860, 1414
BssAI RCCGGY 2 cut(s) 458, 1338
BssECI CCNNGG 9 cut(s) 48, 253, 279, 297, 340, 891, 1043, 1200, 1253
BssMI GATC 9 cut(s) 42, 306, 361, 366, 810, 993, 1005, 1465, 1666
BssNI GRCGYC 2 cut(s) 69, 1136
BssT1I CCWWGG 4 cut(s) 48, 279, 297, 891
Bst2UI CCWGG 2 cut(s) 353, 1261
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 1 cut(s) 924
BstACI GRCGYC 2 cut(s) 69, 1136
BstBI TTCGAA 1 cut(s) 1085
BstC8I GCNNGC 4 cut(s) 114, 261, 639, 783
BstDEI CTNAG 4 cut(s) 525, 537, 1098, 1107
BstDSI CCRYGG 5 cut(s) 253, 279, 297, 340, 1043
BstF5I GGATG 9 cut(s) 251, 508, 901, 1018, 1039, 1225, 1273, 1486, 1510
BstFNI CGCG 6 cut(s) 116, 123, 125, 152, 263, 641
BstH2I RGCGCY 1 cut(s) 833
BstHHI GCGC 3 cut(s) 125, 832, 845
BstKTI GATC 9 cut(s) 45, 309, 364, 369, 813, 996, 1008, 1468, 1669
BstMAI GTCTC 4 cut(s) 238, 807, 1041, 1391
BstMBI GATC 9 cut(s) 42, 306, 361, 366, 810, 993, 1005, 1465, 1666
BstMCI CGRYCG 5 cut(s) 76, 340, 792, 813, 1468
BstNI CCWGG 2 cut(s) 353, 1261
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 7 cut(s) 59, 214, 241, 351, 472, 1259, 1476
BstUI CGCG 6 cut(s) 116, 123, 125, 152, 263, 641
BstV1I GCAGC 5 cut(s) 615, 661, 806, 927, 1064
BstXI CCANNNNNNTGG 2 cut(s) 280, 748
BstZI CGGCCG 1 cut(s) 337
Bsu15I ATCGAT 1 cut(s) 1665
BsuRI GGCC 8 cut(s) 53, 284, 295, 339, 551, 620, 649, 1495
BsuTUI ATCGAT 1 cut(s) 1665
BtgI CCRYGG 5 cut(s) 253, 279, 297, 340, 1043
BtrI CACGTC 1 cut(s) 826
BtsCI GGATG 9 cut(s) 251, 508, 901, 1018, 1039, 1225, 1273, 1486, 1510
BtsI GCAGTG 1 cut(s) 1359
BtsIMutI CAGTG 2 cut(s) 1359, 1387
BtuMI TCGCGA 1 cut(s) 116
Cac8I GCNNGC 4 cut(s) 114, 261, 639, 783
CciI TCATGA 1 cut(s) 775
CfoI GCGC 3 cut(s) 125, 832, 845
Cfr10I RCCGGY 2 cut(s) 458, 1338
Cfr13I GGNCC 6 cut(s) 52, 239, 294, 888, 1257, 1493
ClaI ATCGAT 1 cut(s) 1665
CseI GACGC 3 cut(s) 58, 238, 1144
CsiI ACCWGGT 1 cut(s) 1259
Csp6I GTAC 2 cut(s) 506, 1118
CspCI CAANNNNNGTGG 2 cut(s) 1507, 1542
CviAII CATG 4 cut(s) 79, 280, 298, 776
CviQI GTAC 2 cut(s) 506, 1118
DdeI CTNAG 4 cut(s) 525, 537, 1098, 1107
DpnI GATC 9 cut(s) 44, 308, 363, 368, 812, 995, 1007, 1467, 1668
DpnII GATC 9 cut(s) 42, 306, 361, 366, 810, 993, 1005, 1465, 1666
EaeI YGGCCR 3 cut(s) 282, 337, 549
EagI CGGCCG 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 924
EarI CTCTTC 1 cut(s) 924
EciI GGCGGA 3 cut(s) 388, 545, 1600
Ecl136II GAGCTC 1 cut(s) 358
EclXI CGGCCG 1 cut(s) 337
Eco130I CCWWGG 4 cut(s) 48, 279, 297, 891
Eco24I GRGCYC 3 cut(s) 261, 360, 1181
Eco31I GGTCTC 2 cut(s) 238, 807
Eco47I GGWCC 3 cut(s) 239, 888, 1257
Eco52I CGGCCG 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 1617
Eco88I CYCGRG 3 cut(s) 235, 310, 684
EcoICRI GAGCTC 1 cut(s) 358
EcoO109I RGGNCCY 2 cut(s) 52, 239
EcoRI GAATTC 2 cut(s) 96, 601
EcoRII CCWGG 2 cut(s) 351, 1259
EcoT14I CCWWGG 4 cut(s) 48, 279, 297, 891
EcoT38I GRGCYC 3 cut(s) 261, 360, 1181
ErhI CCWWGG 4 cut(s) 48, 279, 297, 891
FaeI CATG 4 cut(s) 82, 283, 301, 779
FalI AAGNNNNNCTT 2 cut(s) 1566, 1598
FaqI GGGAC 4 cut(s) 225, 1288, 1514, 1603
FatI CATG 4 cut(s) 78, 279, 297, 775
FauI CCCGC 4 cut(s) 166, 256, 1309, 1531
FblI GTMKAC 3 cut(s) 72, 211, 469
FokI GGATG 9 cut(s) 258, 515, 908, 1025, 1026, 1232, 1280, 1493, 1517
FriOI GRGCYC 3 cut(s) 261, 360, 1181
GlaI GCGC 3 cut(s) 124, 831, 844
HaeII RGCGCY 1 cut(s) 833
HaeIII GGCC 8 cut(s) 53, 284, 295, 339, 551, 620, 649, 1495
HgaI GACGC 3 cut(s) 58, 238, 1144
HhaI GCGC 3 cut(s) 125, 832, 845
Hin1I GRCGYC 2 cut(s) 69, 1136
Hin1II CATG 4 cut(s) 82, 283, 301, 779
Hin6I GCGC 3 cut(s) 123, 830, 843
HinP1I GCGC 3 cut(s) 123, 830, 843
HincII GTYRAC 3 cut(s) 73, 212, 470
HindII GTYRAC 3 cut(s) 73, 212, 470
HindIII AAGCTT 1 cut(s) 1560
HinfI GANTC 7 cut(s) 8, 87, 138, 575, 726, 1364, 1622
HphI GGTGA 4 cut(s) 17, 97, 884, 1081
Hpy166II GTNNAC 4 cut(s) 73, 212, 253, 470
Hpy188III TCNNGA 6 cut(s) 115, 776, 814, 1132, 1334, 1368
Hpy8I GTNNAC 4 cut(s) 73, 212, 253, 470
Hpy99I CGWCG 3 cut(s) 74, 380, 502
HpyAV CCTTC 5 cut(s) 577, 748, 1066, 1352, 1636
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 825
HpyF3I CTNAG 4 cut(s) 525, 537, 1098, 1107
HpySE526I ACGT 1 cut(s) 825
Hsp92I GRCGYC 2 cut(s) 69, 1136
Hsp92II CATG 4 cut(s) 82, 283, 301, 779
HspAI GCGC 3 cut(s) 123, 830, 843
KflI GGGWCCC 1 cut(s) 239
Kzo9I GATC 9 cut(s) 42, 306, 361, 366, 810, 993, 1005, 1465, 1666
LmnI GCTCC 5 cut(s) 355, 636, 1176, 1189, 1519
Lsp1109I GCAGC 5 cut(s) 615, 661, 806, 927, 1064
LweI GCATC 4 cut(s) 236, 961, 1147, 1258
MabI ACCWGGT 1 cut(s) 1259
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 5 cut(s) 103, 176, 821, 1304, 1416
MalI GATC 9 cut(s) 44, 308, 363, 368, 812, 995, 1007, 1467, 1668
MbiI CCGCTC 1 cut(s) 689
MboI GATC 9 cut(s) 42, 306, 361, 366, 810, 993, 1005, 1465, 1666
MboII GAAGA 5 cut(s) 941, 1003, 1156, 1482, 1610
MfeI CAATTG 1 cut(s) 1161
MhlI GDGCHC 3 cut(s) 261, 360, 1181
MlsI TGGCCA 2 cut(s) 284, 551
MluNI TGGCCA 2 cut(s) 284, 551
MlyI GAGTC 2 cut(s) 96, 584
MmeI TCCRAC 6 cut(s) 297, 480, 558, 946, 1245, 1276
Mox20I TGGCCA 2 cut(s) 284, 551
MroXI GAANNNNTTC 1 cut(s) 332
MscI TGGCCA 2 cut(s) 284, 551
MseI TTAA 3 cut(s) 852, 1125, 1440
MslI CAYNNNNRTG 3 cut(s) 302, 746, 870
Msp20I TGGCCA 2 cut(s) 284, 551
MspA1I CMGCKG 2 cut(s) 40, 1646
MspR9I CCNGG 7 cut(s) 61, 216, 243, 353, 474, 1261, 1478
MunI CAATTG 1 cut(s) 1161
MvaI CCWGG 2 cut(s) 353, 1261
MvnI CGCG 6 cut(s) 116, 123, 125, 152, 263, 641
NciI CCSGG 5 cut(s) 61, 216, 243, 474, 1478
NcoI CCATGG 2 cut(s) 279, 297
NdeII GATC 9 cut(s) 42, 306, 361, 366, 810, 993, 1005, 1465, 1666
NlaIII CATG 4 cut(s) 82, 283, 301, 779
NlaIV GGNNCC 3 cut(s) 240, 241, 1178
NmeAIII GCCGAG 3 cut(s) 586, 1278, 1610
NmuCI GTSAC 2 cut(s) 103, 821
NruI TCGCGA 1 cut(s) 116
NspI RCATGY 1 cut(s) 82
NspV TTCGAA 1 cut(s) 1085
OliI CACNNNNGTG 1 cut(s) 870
PaeR7I CTCGAG 1 cut(s) 684
PagI TCATGA 1 cut(s) 775
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 5 cut(s) 8, 138, 726, 1364, 1622
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 2 cut(s) 241, 351
Ple19I CGATCG 2 cut(s) 813, 1468
PleI GAGTC 2 cut(s) 95, 583
PpsI GAGTC 2 cut(s) 95, 583
PpuMI RGGWCCY 1 cut(s) 239
PsiI TTATAA 1 cut(s) 1652
Psp124BI GAGCTC 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 351, 1259
PspGI CCWGG 2 cut(s) 351, 1259
PspN4I GGNNCC 3 cut(s) 240, 241, 1178
PspPI GGNCC 6 cut(s) 52, 239, 294, 888, 1257, 1493
PspPPI RGGWCCY 1 cut(s) 239
PspXI VCTCGAGB 1 cut(s) 684
PvuI CGATCG 2 cut(s) 813, 1468
PvuII CAGCTG 2 cut(s) 40, 1646
RruI TCGCGA 1 cut(s) 116
RsaI GTAC 2 cut(s) 507, 1119
RsaNI GTAC 2 cut(s) 506, 1118
RseI CAYNNNNRTG 3 cut(s) 302, 746, 870
SacI GAGCTC 1 cut(s) 360
SalI GTCGAC 3 cut(s) 71, 210, 468
SaqAI TTAA 3 cut(s) 852, 1125, 1440
Sau3AI GATC 9 cut(s) 42, 306, 361, 366, 810, 993, 1005, 1465, 1666
Sau96I GGNCC 6 cut(s) 52, 239, 294, 888, 1257, 1493
SchI GAGTC 2 cut(s) 96, 584
ScrFI CCNGG 7 cut(s) 61, 216, 243, 353, 474, 1261, 1478
SduI GDGCHC 3 cut(s) 261, 360, 1181
SexAI ACCWGGT 1 cut(s) 1259
SfaNI GCATC 4 cut(s) 236, 961, 1147, 1258
Sfr274I CTCGAG 1 cut(s) 684
SfuI TTCGAA 1 cut(s) 1085
SinI GGWCC 3 cut(s) 239, 888, 1257
SlaI CTCGAG 1 cut(s) 684
SmiMI CAYNNNNRTG 3 cut(s) 302, 746, 870
SmlI CTYRAG 2 cut(s) 684, 1604
SmoI CTYRAG 2 cut(s) 684, 1604
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 7 cut(s) 59, 214, 241, 351, 472, 1259, 1476
StyI CCWWGG 4 cut(s) 48, 279, 297, 891
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 828
TaqII GACCGA 2 cut(s) 1218, 1262
TatI WGTACW 1 cut(s) 505
TauI GCSGC 5 cut(s) 185, 374, 560, 788, 880
TfiI GAWTC 5 cut(s) 8, 138, 726, 1364, 1622
Tru1I TTAA 3 cut(s) 852, 1125, 1440
Tru9I TTAA 3 cut(s) 852, 1125, 1440
TscAI CASTG 2 cut(s) 1359, 1387
TseFI GTSAC 2 cut(s) 103, 821
TseI GCWGC 5 cut(s) 628, 674, 794, 940, 1052
Tsp45I GTSAC 2 cut(s) 103, 821
TspDTI ATGAA 3 cut(s) 895, 1157, 1497
TspGWI ACGGA 2 cut(s) 486, 1032
TspRI CASTG 2 cut(s) 1359, 1387
Van91I CCANNNNNTGG 1 cut(s) 291
VpaK11BI GGWCC 3 cut(s) 239, 888, 1257
XapI RAATTY 4 cut(s) 96, 392, 566, 601
XceI RCATGY 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 684
XmiI GTMKAC 3 cut(s) 72, 211, 469
XmnI GAANNNNTTC 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.