MD09G1258100.v1.1

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014551
Physical Location & Seq
Reverse (-)
33013570 .. 33017649
4080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1258100.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGGGTGAATCAACCACAACCGCACTCGATGGCAACCAGCTGATCGCCAAGGCCCTTGCCCGATTCGGCGTCGACCACATGTTCGGCGTCGTGGGAATTCCTGTTACCTCGCTCGCGAACCGTGCGGTCTCGCTCGGAGTCCGGTTTATTGCCTTCCACAATGAGCAGTCCGCGGGTTACGCCGCCTCCGCCTACGGGTATCTAACTGGTCGACCCGGGGTCCTCCTCACGGTCTCGGGTCCCGGATGCGTCCACGGGCTCGCCGGTCTTTCCAACGCCATGACCAATGCTTGGCCCATGGTGATGATCTCTGGTTCGTGCGACCAGAAAGATTTCGGCCGCGGCGATTTCCAGGAGCTCGATCAGATCGCAGCCGTCGAACCCTTTTCCAAATTCTCCGCCAAAGCTAAATCAATCAAAGAAATCCCGGATTGCGTGTTTCAGGCTCTCGCTGAAGCCGGTTCGGGTCGACCCGGAGGGTGCTATTTGGATTTTCCGTCGGATGTTCTGCACCAGACAATTTCTGAGGCGGAGGCTGAGAGCTTATTGGCCACCGCTGCCGATAAATTCGGAGAGTCGGAGTCGGAGAAGGTTCTGAAAGTACAGAATTCGCAGATTGATGAAGCGGTTTCGCTGCTACGGCACGCGGAGCGGCCGTTGATTGTGTTTGGGAAGGGAGCTGCATTTGCTCGAGCGGAGAATGAGTTGGGGAAACTAGTGGAGAGGACCGGAATCCCATTTTTGCCCACTCCAATGGGGAAGGGACTGTTGCCTGATACTCATGAGCTGGCGGCGACGGCAGCGAGGTCTCTGGCGATCGGCAAATGTGATGTGGCGCTCGTTGTCGGCGCCAGGCTTAACTGGCTTTTGCACTTTGGTGAGCCTCCCAAATGGTCTAAGGATGTGAAGTTCATTTTGGTTGATGTTAGTAAGGAAGAGATTGAGCTGCGAAAACCGCATTTGGGTTTGGTTGGAGATGCAAAGTTGGTGTTGGAAAAGATCAATTTGGGGATCAAGGATGATCCTTTTTGTTTGGGGAGGTCTCATTCGTGGGTTGCAGCGATTTCAAGTAAGGTGAAGGACAATGTTTCAAAAATGGAGGCTCAGTTGGCTAAGGAAGTTGTGCCATTTAATTTCTTGACGCCAATGAAGATTATTAGAGATGCAATTGCAGGGTTGGGGAGCCCTGCTCCTATACTGGTTTCTGAGGGGGCTAACACCATGGATGTGGGTCGGTCTGTGTTGGTTCAGACCGAGCCAAGGACCAGGTTGGATGCAGGGACTTGGGGAACAATGGGGGTTGGTTTGGGTTACTGCATTGCGGCTGCGGTGGCTTCTCCTGACCGGCTTGTTGTTGCAGTGGAAGGGGATTCAGGTTTTGGGTTCAGTGCAATGGAAGTTGAGACATTGGTTCGATACCAGTTACCTGTTGTCGTGATAGTCTTTAACAATGGTGGTGTATATGGTGGTGATCGAAGAAGCCCAGATGAAATTAGTGGGCCTTACAAAGATGACCCTGCTCCTACTTCCTTCATCCCAAATGCGGGTTATCACACTCTGATTGAAGCATTTGGAGGCAAAGGATATCTTGTTGGGACACCTGAAGAACTCAAGTCTGCCATTTCAGAATCATTCTCCGCACGAAAACCTGCTGTTATAAATGTAGTTATTGATCCTTATGCTGGTGCAGAAAGTGGGAGGCTTCAACATAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

573

Amino Acids

60.68

Weight (kDa)

5.68

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 10 - 124 7.7e-32 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 206 - 334 9.3e-34 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 407 - 555 6.4e-27 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17380
fragaria_vesca FvH4_1g28990
malus_domestica MD00G1054500.v1.1 MD09G1258100.v1.1
prunus_persica Prupe.3G150000_v2.0.a1
pyrus_communis pycom09g17290 pycom17g25510
rosa_chinensis RchiOBHm_Chr3g0494041
rosa_laevigata RLG00000022686
rosa_multiflora Rmu_sc0003412.1_g000008
rosa_roxburghii Rroxscaffold_6G00391310
rosa_rugosa Rorug03G0263700
rosa_samantha Rh3AG313400 Rh3BG349500 Rh3CG346300 Rh3DG348800
rosa_wichuraiana Rw3G027430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1658
Acc36I ACCTGC 1 cut(s) 1657
AccB1I GGYRCC 1 cut(s) 848
AccB7I CCANNNNNTGG 1 cut(s) 291
AccBSI CCGCTC 2 cut(s) 652, 695
AccI GTMKAC 3 cut(s) 72, 211, 469
AccII CGCG 4 cut(s) 116, 173, 342, 647
AclWI GGATC 3 cut(s) 1016, 1019, 1667
AcoI YGGCCR 3 cut(s) 337, 549, 653
AcsI RAATTY 4 cut(s) 96, 392, 566, 607
AcuI CTGAAG 2 cut(s) 474, 1623
AcyI GRCGYC 4 cut(s) 69, 87, 849, 1142
AfaI GTAC 1 cut(s) 603
AfiI CCNNNNNNNGG 8 cut(s) 195, 229, 291, 962, 1260, 1543, 1544, 1682
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 4 cut(s) 1068, 1092, 1565, 1706
AhdI GACNNNNNGTC 1 cut(s) 218
AhlI ACTAGT 1 cut(s) 715
AjnI CCWGG 3 cut(s) 351, 851, 1265
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AleI CACNNNNGTG 1 cut(s) 876
AloI GAACNNNNNNTCC 2 cut(s) 893, 925
AluBI AGCT 7 cut(s) 40, 358, 407, 543, 680, 787, 946
AluI AGCT 7 cut(s) 40, 358, 407, 543, 680, 787, 946
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 5 cut(s) 133, 238, 813, 1047, 1397
AlwI GGATC 3 cut(s) 1016, 1019, 1667
Ama87I CYCGRG 3 cut(s) 215, 235, 690
AoxI GGCC 6 cut(s) 51, 293, 337, 549, 653, 1499
ApeKI GCWGC 8 cut(s) 371, 557, 634, 680, 800, 946, 1058, 1325
ApoI RAATTY 4 cut(s) 96, 392, 566, 607
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp700I GAANNNNTTC 1 cut(s) 332
AspLEI GCGC 2 cut(s) 838, 851
AspS9I GGNCC 7 cut(s) 52, 220, 239, 294, 726, 1263, 1499
AsuC2I CCSGG 5 cut(s) 216, 217, 243, 428, 474
AsuHPI GGTGA 5 cut(s) 17, 313, 890, 1087, 1481
AvaI CYCGRG 3 cut(s) 215, 235, 690
AvaII GGWCC 4 cut(s) 220, 239, 726, 1263
BalI TGGCCA 1 cut(s) 551
BanI GGYRCC 1 cut(s) 848
BanII GRGCYC 3 cut(s) 261, 360, 1187
Bbv12I GWGCWC 1 cut(s) 360
BbvI GCAGC 8 cut(s) 383, 544, 621, 667, 812, 933, 1070, 1312
BccI CCATC 1 cut(s) 23
BceAI ACGGC 4 cut(s) 359, 640, 656, 813
BciT130I CCWGG 3 cut(s) 353, 853, 1267
BcnI CCSGG 5 cut(s) 216, 217, 243, 428, 474
BcoDI GTCTC 5 cut(s) 133, 238, 813, 1047, 1397
BcuI ACTAGT 1 cut(s) 715
BfaI CTAG 1 cut(s) 716
BfoI RGCGCY 2 cut(s) 839, 852
BfuAI ACCTGC 1 cut(s) 1657
Bme1390I CCNGG 8 cut(s) 216, 217, 243, 353, 428, 474, 853, 1267
Bme18I GGWCC 4 cut(s) 220, 239, 726, 1263
BmeRI GACNNNNNGTC 1 cut(s) 218
BmeT110I CYCGRG 3 cut(s) 215, 235, 690
BmgT120I GGNCC 7 cut(s) 52, 220, 239, 294, 726, 1263, 1499
BmiI GGNNCC 5 cut(s) 221, 240, 241, 850, 1184
BmrFI CCNGG 8 cut(s) 216, 217, 243, 353, 428, 474, 853, 1267
BmsI GCATC 4 cut(s) 236, 967, 1153, 1264
BplI GAGNNNNNCTC 2 cut(s) 1174, 1206
Bpu10I CCTNAGC 1 cut(s) 1113
BpuEI CTTGAG 1 cut(s) 1595
BpuMI CCSGG 5 cut(s) 216, 217, 243, 428, 474
BsaHI GRCGYC 4 cut(s) 69, 87, 849, 1142
BsaI GGTCTC 4 cut(s) 133, 238, 813, 1047
BsaJI CCNNGG 9 cut(s) 48, 171, 215, 216, 253, 297, 340, 1221, 1259
BsaWI WCCGGW 2 cut(s) 141, 728
BsaXI ACNNNNNCTCC 8 cut(s) 170, 200, 641, 671, 689, 719, 1091, 1121
Bsc4I CCNNNNNNNGG 8 cut(s) 195, 229, 291, 962, 1260, 1543, 1544, 1682
Bse118I RCCGGY 3 cut(s) 263, 458, 1344
Bse1I ACTGG 4 cut(s) 211, 866, 1203, 1420
Bse3DI GCAATG 2 cut(s) 1317, 1398
BseBI CCWGG 3 cut(s) 353, 853, 1267
BseDI CCNNGG 9 cut(s) 48, 171, 215, 216, 253, 297, 340, 1221, 1259
BseGI GGATG 7 cut(s) 251, 508, 907, 1024, 1231, 1279, 1533
BseLI CCNNNNNNNGG 8 cut(s) 195, 229, 291, 962, 1260, 1543, 1544, 1682
BseMI GCAATG 2 cut(s) 1317, 1398
BseMII CTCAG 4 cut(s) 516, 528, 1118, 1197
BseNI ACTGG 4 cut(s) 211, 866, 1203, 1420
BseRI GAGGAG 1 cut(s) 215
BseX3I CGGCCG 2 cut(s) 337, 653
BseXI GCAGC 8 cut(s) 383, 544, 621, 667, 812, 933, 1070, 1312
BsgI GTGCAG 2 cut(s) 494, 1707
Bsh1236I CGCG 4 cut(s) 116, 173, 342, 647
Bsh1285I CGRYCG 3 cut(s) 340, 656, 819
BshFI GGCC 6 cut(s) 53, 295, 339, 551, 655, 1501
BshNI GGYRCC 1 cut(s) 848
BsiEI CGRYCG 3 cut(s) 340, 656, 819
BsiHKAI GWGCWC 1 cut(s) 360
BsiHKCI CYCGRG 3 cut(s) 215, 235, 690
BsiSI CCGG 9 cut(s) 142, 216, 243, 264, 428, 459, 474, 729, 1345
BslFI GGGAC 4 cut(s) 225, 777, 1294, 1609
BslI CCNNNNNNNGG 8 cut(s) 195, 229, 291, 962, 1260, 1543, 1544, 1682
BsmAI GTCTC 5 cut(s) 133, 238, 813, 1047, 1397
BsmFI GGGAC 4 cut(s) 225, 777, 1294, 1609
BsnI GGCC 6 cut(s) 53, 295, 339, 551, 655, 1501
Bso31I GGTCTC 4 cut(s) 133, 238, 813, 1047
BsoBI CYCGRG 3 cut(s) 215, 235, 690
Bsp1286I GDGCHC 3 cut(s) 261, 360, 1187
Bsp19I CCATGG 2 cut(s) 297, 1221
Bsp68I TCGCGA 1 cut(s) 116
BspANI GGCC 6 cut(s) 53, 295, 339, 551, 655, 1501
BspCNI CTCAG 4 cut(s) 517, 529, 1117, 1198
BspFNI CGCG 4 cut(s) 116, 173, 342, 647
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 5 cut(s) 221, 240, 241, 850, 1184
BspMI ACCTGC 1 cut(s) 1657
BspPI GGATC 3 cut(s) 1016, 1019, 1667
BspT107I GGYRCC 1 cut(s) 848
BspTNI GGTCTC 4 cut(s) 133, 238, 813, 1047
BsrBI CCGCTC 2 cut(s) 652, 695
BsrDI GCAATG 2 cut(s) 1317, 1398
BsrFI RCCGGY 3 cut(s) 263, 458, 1344
BsrI ACTGG 4 cut(s) 211, 866, 1203, 1420
BssAI RCCGGY 3 cut(s) 263, 458, 1344
BssECI CCNNGG 9 cut(s) 48, 171, 215, 216, 253, 297, 340, 1221, 1259
BssNI GRCGYC 4 cut(s) 69, 87, 849, 1142
BssT1I CCWWGG 4 cut(s) 48, 297, 1221, 1259
Bst2UI CCWGG 3 cut(s) 353, 853, 1267
Bst4CI ACNGT 3 cut(s) 122, 232, 768
Bst6I CTCTTC 1 cut(s) 930
BstACI GRCGYC 4 cut(s) 69, 87, 849, 1142
BstC8I GCNNGC 4 cut(s) 114, 261, 645, 789
BstDEI CTNAG 6 cut(s) 525, 537, 897, 1104, 1113, 1206
BstDSI CCRYGG 5 cut(s) 171, 253, 297, 340, 1221
BstF5I GGATG 7 cut(s) 251, 508, 907, 1024, 1231, 1279, 1533
BstFNI CGCG 4 cut(s) 116, 173, 342, 647
BstH2I RGCGCY 2 cut(s) 839, 852
BstHHI GCGC 2 cut(s) 838, 851
BstMAI GTCTC 5 cut(s) 133, 238, 813, 1047, 1397
BstMCI CGRYCG 3 cut(s) 340, 656, 819
BstNI CCWGG 3 cut(s) 353, 853, 1267
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 8 cut(s) 214, 215, 241, 351, 426, 472, 851, 1265
BstUI CGCG 4 cut(s) 116, 173, 342, 647
BstV1I GCAGC 8 cut(s) 383, 544, 621, 667, 812, 933, 1070, 1312
BstXI CCANNNNNNTGG 2 cut(s) 754, 1228
BstZI CGGCCG 2 cut(s) 337, 653
BsuRI GGCC 6 cut(s) 53, 295, 339, 551, 655, 1501
BtgI CCRYGG 5 cut(s) 171, 253, 297, 340, 1221
BtsCI GGATG 7 cut(s) 251, 508, 907, 1024, 1231, 1279, 1533
BtsI GCAGTG 1 cut(s) 1365
BtsIMutI CAGTG 2 cut(s) 1365, 1393
BtuMI TCGCGA 1 cut(s) 116
BveI ACCTGC 1 cut(s) 1657
Cac8I GCNNGC 4 cut(s) 114, 261, 645, 789
CciI TCATGA 1 cut(s) 781
CfoI GCGC 2 cut(s) 838, 851
Cfr10I RCCGGY 3 cut(s) 263, 458, 1344
Cfr13I GGNCC 7 cut(s) 52, 220, 239, 294, 726, 1263, 1499
Cfr42I CCGCGG 2 cut(s) 174, 343
Cfr9I CCCGGG 1 cut(s) 215
CseI GACGC 4 cut(s) 58, 76, 238, 1150
CsiI ACCWGGT 1 cut(s) 1265
Csp6I GTAC 1 cut(s) 602
CviAII CATG 5 cut(s) 79, 280, 298, 782, 1222
CviQI GTAC 1 cut(s) 602
DdeI CTNAG 6 cut(s) 525, 537, 897, 1104, 1113, 1206
DinI GGCGCC 1 cut(s) 850
DriI GACNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 3 cut(s) 337, 549, 653
EagI CGGCCG 2 cut(s) 337, 653
Eam1104I CTCTTC 1 cut(s) 930
Eam1105I GACNNNNNGTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 930
EciI GGCGGA 3 cut(s) 178, 388, 545
Ecl136II GAGCTC 1 cut(s) 358
EclXI CGGCCG 2 cut(s) 337, 653
Eco130I CCWWGG 4 cut(s) 48, 297, 1221, 1259
Eco24I GRGCYC 3 cut(s) 261, 360, 1187
Eco31I GGTCTC 4 cut(s) 133, 238, 813, 1047
Eco32I GATATC 1 cut(s) 1586
Eco47I GGWCC 4 cut(s) 220, 239, 726, 1263
Eco52I CGGCCG 2 cut(s) 337, 653
Eco53kI GAGCTC 1 cut(s) 358
Eco57I CTGAAG 2 cut(s) 474, 1623
Eco88I CYCGRG 3 cut(s) 215, 235, 690
EcoICRI GAGCTC 1 cut(s) 358
EcoO109I RGGNCCY 3 cut(s) 52, 220, 239
EcoRI GAATTC 2 cut(s) 96, 607
EcoRII CCWGG 3 cut(s) 351, 851, 1265
EcoRV GATATC 1 cut(s) 1586
EcoT14I CCWWGG 4 cut(s) 48, 297, 1221, 1259
EcoT38I GRGCYC 3 cut(s) 261, 360, 1187
EgeI GGCGCC 1 cut(s) 850
EheI GGCGCC 1 cut(s) 850
ErhI CCWWGG 4 cut(s) 48, 297, 1221, 1259
FaeI CATG 5 cut(s) 82, 283, 301, 785, 1225
FalI AAGNNNNNCTT 2 cut(s) 1572, 1604
FaqI GGGAC 4 cut(s) 225, 777, 1294, 1609
FatI CATG 5 cut(s) 78, 279, 297, 781, 1221
FauI CCCGC 2 cut(s) 166, 1537
FblI GTMKAC 3 cut(s) 72, 211, 469
FokI GGATG 7 cut(s) 258, 515, 914, 1031, 1238, 1286, 1520
FriOI GRGCYC 3 cut(s) 261, 360, 1187
FspBI CTAG 1 cut(s) 716
GlaI GCGC 2 cut(s) 837, 850
HaeII RGCGCY 2 cut(s) 839, 852
HaeIII GGCC 6 cut(s) 53, 295, 339, 551, 655, 1501
HapII CCGG 9 cut(s) 142, 216, 243, 264, 428, 459, 474, 729, 1345
HgaI GACGC 4 cut(s) 58, 76, 238, 1150
HhaI GCGC 2 cut(s) 838, 851
Hin1I GRCGYC 4 cut(s) 69, 87, 849, 1142
Hin1II CATG 5 cut(s) 82, 283, 301, 785, 1225
Hin6I GCGC 2 cut(s) 836, 849
HinP1I GCGC 2 cut(s) 836, 849
HincII GTYRAC 3 cut(s) 73, 212, 470
HindII GTYRAC 3 cut(s) 73, 212, 470
HinfI GANTC 8 cut(s) 8, 63, 138, 575, 581, 732, 1370, 1628
HpaII CCGG 9 cut(s) 142, 216, 243, 264, 428, 459, 474, 729, 1345
HphI GGTGA 5 cut(s) 17, 313, 890, 1087, 1481
Hpy166II GTNNAC 4 cut(s) 73, 212, 253, 470
Hpy188III TCNNGA 5 cut(s) 115, 782, 1138, 1340, 1435
Hpy8I GTNNAC 4 cut(s) 73, 212, 253, 470
Hpy99I CGWCG 5 cut(s) 74, 92, 380, 502, 799
HpyAV CCTTC 7 cut(s) 163, 583, 667, 754, 1072, 1358, 1540
HpyCH4III ACNGT 3 cut(s) 122, 232, 768
HpyF3I CTNAG 6 cut(s) 525, 537, 897, 1104, 1113, 1206
Hsp92I GRCGYC 4 cut(s) 69, 87, 849, 1142
Hsp92II CATG 5 cut(s) 82, 283, 301, 785, 1225
HspAI GCGC 2 cut(s) 836, 849
KasI GGCGCC 1 cut(s) 848
KflI GGGWCCC 1 cut(s) 239
KspI CCGCGG 2 cut(s) 174, 343
LmnI GCTCC 6 cut(s) 355, 649, 677, 1182, 1195, 1525
Lsp1109I GCAGC 8 cut(s) 383, 544, 621, 667, 812, 933, 1070, 1312
LweI GCATC 4 cut(s) 236, 967, 1153, 1264
MabI ACCWGGT 1 cut(s) 1265
MaeI CTAG 1 cut(s) 716
MaeIII GTNAC 4 cut(s) 103, 176, 1310, 1422
MbiI CCGCTC 2 cut(s) 652, 695
MboII GAAGA 4 cut(s) 947, 1162, 1488, 1616
MfeI CAATTG 1 cut(s) 1167
MhlI GDGCHC 3 cut(s) 261, 360, 1187
MlsI TGGCCA 1 cut(s) 551
MluCI AATT 9 cut(s) 96, 392, 519, 566, 607, 1003, 1132, 1167, 1491
MluNI TGGCCA 1 cut(s) 551
Mly113I GGCGCC 1 cut(s) 849
MlyI GAGTC 3 cut(s) 147, 584, 590
MmeI TCCRAC 7 cut(s) 297, 480, 558, 564, 952, 972, 1251
Mox20I TGGCCA 1 cut(s) 551
MroXI GAANNNNTTC 1 cut(s) 332
MscI TGGCCA 1 cut(s) 551
MseI TTAA 3 cut(s) 858, 1131, 1446
MslI CAYNNNNRTG 4 cut(s) 302, 752, 876, 1226
Msp20I TGGCCA 1 cut(s) 551
MspA1I CMGCKG 4 cut(s) 40, 173, 342, 557
MspI CCGG 9 cut(s) 142, 216, 243, 264, 428, 459, 474, 729, 1345
MspR9I CCNGG 8 cut(s) 216, 217, 243, 353, 428, 474, 853, 1267
MunI CAATTG 1 cut(s) 1167
MvaI CCWGG 3 cut(s) 353, 853, 1267
MvnI CGCG 4 cut(s) 116, 173, 342, 647
NarI GGCGCC 1 cut(s) 849
NciI CCSGG 5 cut(s) 216, 217, 243, 428, 474
NcoI CCATGG 2 cut(s) 297, 1221
NlaIII CATG 5 cut(s) 82, 283, 301, 785, 1225
NlaIV GGNNCC 5 cut(s) 221, 240, 241, 850, 1184
NruI TCGCGA 1 cut(s) 116
NspI RCATGY 1 cut(s) 82
OliI CACNNNNGTG 1 cut(s) 876
PaeR7I CTCGAG 1 cut(s) 690
PagI TCATGA 1 cut(s) 781
PciI ACATGT 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 342, 375
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 5 cut(s) 8, 63, 732, 1370, 1628
PflMI CCANNNNNTGG 1 cut(s) 291
PfoI TCCNGGA 3 cut(s) 241, 351, 426
Ple19I CGATCG 1 cut(s) 819
PleI GAGTC 3 cut(s) 146, 583, 589
PluTI GGCGCC 1 cut(s) 852
PpsI GAGTC 3 cut(s) 146, 583, 589
PpuMI RGGWCCY 2 cut(s) 220, 239
PscI ACATGT 1 cut(s) 78
PsiI TTATAA 1 cut(s) 1658
Psp124BI GAGCTC 1 cut(s) 360
Psp5II RGGWCCY 2 cut(s) 220, 239
Psp6I CCWGG 3 cut(s) 351, 851, 1265
PspGI CCWGG 3 cut(s) 351, 851, 1265
PspN4I GGNNCC 5 cut(s) 221, 240, 241, 850, 1184
PspPI GGNCC 7 cut(s) 52, 220, 239, 294, 726, 1263, 1499
PspPPI RGGWCCY 2 cut(s) 220, 239
PspXI VCTCGAGB 1 cut(s) 690
PvuI CGATCG 1 cut(s) 819
PvuII CAGCTG 1 cut(s) 40
RruI TCGCGA 1 cut(s) 116
RsaI GTAC 1 cut(s) 603
RsaNI GTAC 1 cut(s) 602
RseI CAYNNNNRTG 4 cut(s) 302, 752, 876, 1226
SacI GAGCTC 1 cut(s) 360
SacII CCGCGG 2 cut(s) 174, 343
SalI GTCGAC 3 cut(s) 71, 210, 468
SaqAI TTAA 3 cut(s) 858, 1131, 1446
Sau96I GGNCC 7 cut(s) 52, 220, 239, 294, 726, 1263, 1499
SchI GAGTC 3 cut(s) 147, 584, 590
ScrFI CCNGG 8 cut(s) 216, 217, 243, 353, 428, 474, 853, 1267
SduI GDGCHC 3 cut(s) 261, 360, 1187
SexAI ACCWGGT 1 cut(s) 1265
SfaNI GCATC 4 cut(s) 236, 967, 1153, 1264
SfoI GGCGCC 1 cut(s) 850
Sfr274I CTCGAG 1 cut(s) 690
Sfr303I CCGCGG 2 cut(s) 174, 343
SgrBI CCGCGG 2 cut(s) 174, 343
SinI GGWCC 4 cut(s) 220, 239, 726, 1263
SlaI CTCGAG 1 cut(s) 690
SmaI CCCGGG 1 cut(s) 217
SmiMI CAYNNNNRTG 4 cut(s) 302, 752, 876, 1226
SmlI CTYRAG 2 cut(s) 690, 1610
SmoI CTYRAG 2 cut(s) 690, 1610
SpeI ACTAGT 1 cut(s) 715
Sse9I AATT 9 cut(s) 96, 392, 519, 566, 607, 1003, 1132, 1167, 1491
SspDI GGCGCC 1 cut(s) 848
SspMI CTAG 1 cut(s) 716
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 8 cut(s) 214, 215, 241, 351, 426, 472, 851, 1265
StyI CCWWGG 4 cut(s) 48, 297, 1221, 1259
TaaI ACNGT 3 cut(s) 122, 232, 768
TaqI TCGA 9 cut(s) 27, 72, 211, 360, 378, 469, 691, 1414, 1474
TaqII GACCGA 2 cut(s) 1224, 1268
TasI AATT 9 cut(s) 96, 392, 519, 566, 607, 1003, 1132, 1167, 1491
TatI WGTACW 1 cut(s) 601
TauI GCSGC 6 cut(s) 185, 342, 345, 655, 794, 1325
TfiI GAWTC 5 cut(s) 8, 63, 732, 1370, 1628
Tru1I TTAA 3 cut(s) 858, 1131, 1446
Tru9I TTAA 3 cut(s) 858, 1131, 1446
TscAI CASTG 2 cut(s) 1365, 1393
TseI GCWGC 8 cut(s) 371, 557, 634, 680, 800, 946, 1058, 1325
TspDTI ATGAA 5 cut(s) 636, 901, 1163, 1503, 1522
TspGWI ACGGA 1 cut(s) 486
TspMI CCCGGG 1 cut(s) 215
TspRI CASTG 2 cut(s) 1365, 1393
Van91I CCANNNNNTGG 1 cut(s) 291
VpaK11BI GGWCC 4 cut(s) 220, 239, 726, 1263
XapI RAATTY 4 cut(s) 96, 392, 566, 607
XceI RCATGY 1 cut(s) 82
XhoI CTCGAG 1 cut(s) 690
XmaI CCCGGG 1 cut(s) 215
XmiI GTMKAC 3 cut(s) 72, 211, 469
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.