Rh3CG346300

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0014551
Physical Location & Seq
Forward (+)
39390712 .. 39395592
4881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG346300.1

Sequence Viewer

Length: 1734 bp
ATGGCGGAGTCAGACACCCACAATTTACAAACCGTCGTCCTTGATGGCAACCAGCTGATTGCCAAGTCCCTGGCCCGTTTCGGCGTAGACCGCATGTTCGGCGTGGTGGGCATCCCTGTCACCTCCCTAGCAAACCGCGCAGTCTCGCTTGGTGTCCGATTCCTCGCCTTCCATAACGAGCAGTCCGCCGGCTATGCAGCCTCCGCCTACGGTTACCTAACGGGCCGCCCGGGCGTCCTCCTCACCGTTTCAGGCCCTGGCTGCGTCCACGGGCTGGCGGGCCTCTCCAACGCCATGGCCAATGGGTGGCCCATGGTCATGATCTCCGGCTCCTGTGATCAGAAAGATTTCGGCCGCGGCGATTTCCAGGAGCTCGATCAGATGGCAGCCGTGAAACCCTTTTCCAAGTTCGCTGTTAAAGCCAAGGATATTAACGAGATACCCGAATGCGTGTTGCAGGCTCTTGCTCATGCCGGTTCGGCCCGACCCGGCGGTTGTTATTTGGATTTCCCGTCGGATGTTCTACACCAGACTATTTCCGATTCGGAGGCTGAGGGCCTACTGGCCGGTGCTGCCGAGAGATTCCGAAAATTATCGCAAGAGGTGGTTTTCAATGTGCAGAATTCGCAGATTGAGGAAGCGGCTTCACTGCTTCGACACGCGGAGAGGCCTCTGATTGTGTTTGGTAAAGGAGCGGCGTTTGCTCGAGCTGAGAGTGCTCTGACGAAGCTAGTTGAGACCACCGGAATCCCATTCCTGCCTACTCCTATGGGAAAGGGATTGTTGCCCGATACCCACGACCTGGCGGCAACCGCGGCGAGGTCACTGGCCATCGGCAAATGTGACGTTGCGCTTGTGGTTGGCGCAAGGCTTAACTGGCTCCTGCACTTTGGTGAGCCACCCAAGTGGTCCAAGGACGTCAAGTTCATTTTGGTTGATGTTTGCCAGGAAGAGATTGAGCTCAGAAAACCTCATCTGGGTTTGGTTGGTGATGCCAAATTGGTTTTGGAAAAATTGAATAAGCAGATTAAAGATGACCCCTTTTGTTTGGGGAGGTCTCATCCATGGGTCGAAGCAATTTCCCGCAAGGCCAAGGAGAACGTTTTGAAAATGGAGGCCCAATTGGCTAAACAAGTTGTGCCCTTTAATTTCTTGACACCAATGAAAATTATTAGAGATGCAATTTTGGGACTGGGTACCCCTGCTCCTATACTGGTTTCTGAGGGGGCAAACACCATGGATGTTGGCCGAGCTGTATTGGTTCAGACTGAACCGAGGACCAGATTGGATGCAGGGACTTGGGGGACAATGGGGGTGGGTCTTGGTTACTGCATTGCAGCTGCTGTGGCTTCTCCTGACCGGCTTGTGGTTGCGGTGGAAGGAGATTCTGGATTTGGGTTCAGTGCAATGGAAGTTGAGACATTGGTTCGGTACCAGTTGCCTGTTGTTGTGATTGTCTTCAACAATGGTGGTGTATACGGTGGTGACCGAAGAAGCCAAGATGAAATAAATGGGCCTTACAAAGATGACCCTGCTCCCACTTCTTTCGTCCCGAGTGCCGCTTATCACACACTGATTGAAGCTTTTGGAGGCAAAGGTTATCTTGCTGGGACACCTGAGGAACTCAAGTCTGCCCTTGCGGAATCCTTTGCAGCAAGAAAACCAGCTGTTATAAATGTAATTATTGATCCTTATGCTGGTTCAGAAAGTGGGAGACTTCAACATAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

577

Amino Acids

61.61

Weight (kDa)

6.1

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 16 - 129 6e-31 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 211 - 339 4.8e-34 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 409 - 560 2.6e-27 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17380
fragaria_vesca FvH4_1g28990
malus_domestica MD00G1054500.v1.1 MD09G1258100.v1.1
prunus_persica Prupe.3G150000_v2.0.a1
pyrus_communis pycom09g17290 pycom17g25510
rosa_chinensis RchiOBHm_Chr3g0494041
rosa_laevigata RLG00000022686
rosa_multiflora Rmu_sc0003412.1_g000008
rosa_roxburghii Rroxscaffold_6G00391310
rosa_rugosa Rorug03G0263700
rosa_samantha Rh3AG313400 Rh3BG349500 Rh3CG346300 Rh3DG348800
rosa_wichuraiana Rw3G027430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1673
AatII GACGTC 1 cut(s) 921
Acc65I GGTACC 2 cut(s) 1196, 1431
AccB1I GGYRCC 2 cut(s) 1196, 1431
AccB7I CCANNNNNTGG 3 cut(s) 274, 306, 802
AccBSI CCGCTC 1 cut(s) 695
AccI GTMKAC 2 cut(s) 87, 1476
AccII CGCG 4 cut(s) 138, 357, 662, 815
AclI AACGTT 1 cut(s) 1101
AclWI GGATC 1 cut(s) 1682
AcoI YGGCCR 5 cut(s) 297, 352, 564, 828, 1246
AcsI RAATTY 1 cut(s) 622
AcyI GRCGYC 2 cut(s) 234, 918
AfaI GTAC 2 cut(s) 1198, 1433
AfiI CCNNNNNNNGG 7 cut(s) 274, 306, 802, 819, 977, 1366, 1697
AgsI TTSAA 6 cut(s) 613, 1018, 1108, 1462, 1580, 1721
AjnI CCWGG 5 cut(s) 69, 256, 366, 801, 945
AleI CACNNNNGTG 2 cut(s) 891, 904
AloI GAACNNNNNNTCC 2 cut(s) 908, 940
AluBI AGCT 9 cut(s) 55, 373, 710, 730, 961, 1253, 1340, 1583, 1667
AluI AGCT 9 cut(s) 55, 373, 710, 730, 961, 1253, 1340, 1583, 1667
Alw21I GWGCWC 3 cut(s) 375, 721, 963
Alw26I GTCTC 5 cut(s) 148, 731, 1062, 1412, 1708
AlwI GGATC 1 cut(s) 1682
AlwNI CAGNNNCTG 2 cut(s) 257, 1343
Ama87I CYCGRG 3 cut(s) 229, 705, 1552
ApeKI GCWGC 7 cut(s) 197, 261, 386, 572, 1337, 1340, 1652
ApoI RAATTY 1 cut(s) 622
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
Asp700I GAANNNNTTC 1 cut(s) 347
Asp718I GGTACC 2 cut(s) 1196, 1431
AspLEI GCGC 3 cut(s) 140, 853, 866
AsuC2I CCSGG 3 cut(s) 230, 231, 489
AsuHPI GGTGA 5 cut(s) 112, 235, 905, 1001, 1496
AvaI CYCGRG 3 cut(s) 229, 705, 1552
AvaII GGWCC 2 cut(s) 909, 1278
AxyI CCTNAGG 1 cut(s) 1617
BaeGI GKGCMC 1 cut(s) 1143
BalI TGGCCA 2 cut(s) 299, 830
BanI GGYRCC 2 cut(s) 1196, 1431
BanII GRGCYC 2 cut(s) 375, 963
BbsI GAAGAC 1 cut(s) 1450
Bbv12I GWGCWC 3 cut(s) 375, 721, 963
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 7 cut(s) 209, 248, 398, 559, 1327, 1349, 1664
BccI CCATC 3 cut(s) 38, 376, 839
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 5 cut(s) 71, 258, 368, 803, 947
BclI TGATCA 1 cut(s) 337
BcnI CCSGG 3 cut(s) 230, 231, 489
BcoDI GTCTC 5 cut(s) 148, 731, 1062, 1412, 1708
BfaI CTAG 2 cut(s) 128, 731
BglI GCCNNNNNGGC 3 cut(s) 231, 479, 1124
Bme1390I CCNGG 8 cut(s) 71, 230, 231, 258, 368, 489, 803, 947
Bme18I GGWCC 2 cut(s) 909, 1278
BmeT110I CYCGRG 3 cut(s) 229, 705, 1552
BmiI GGNNCC 4 cut(s) 331, 881, 1198, 1433
BmrFI CCNGG 8 cut(s) 71, 230, 231, 258, 368, 489, 803, 947
BmrI ACTGGG 1 cut(s) 1202
BmsI GCATC 4 cut(s) 120, 982, 1168, 1279
BmuI ACTGGG 1 cut(s) 1202
BpiI GAAGAC 1 cut(s) 1450
Bpu10I CCTNAGC 1 cut(s) 552
BpuEI CTTGAG 1 cut(s) 1610
BpuMI CCSGG 3 cut(s) 230, 231, 489
BsaHI GRCGYC 2 cut(s) 234, 918
BsaI GGTCTC 2 cut(s) 731, 1062
BsaWI WCCGGW 1 cut(s) 743
BsaXI ACNNNNNCTCC 2 cut(s) 1189, 1219
Bsc4I CCNNNNNNNGG 7 cut(s) 274, 306, 802, 819, 977, 1366, 1697
Bse118I RCCGGY 4 cut(s) 188, 473, 566, 1359
Bse1I ACTGG 6 cut(s) 567, 831, 881, 1197, 1218, 1435
Bse21I CCTNAGG 1 cut(s) 1617
Bse3DI GCAATG 2 cut(s) 1332, 1413
BseBI CCWGG 5 cut(s) 71, 258, 368, 803, 947
BseGI GGATG 5 cut(s) 111, 523, 1060, 1246, 1294
BseLI CCNNNNNNNGG 7 cut(s) 274, 306, 802, 819, 977, 1366, 1697
BseMI GCAATG 2 cut(s) 1332, 1413
BseMII CTCAG 5 cut(s) 543, 702, 976, 1212, 1608
BseNI ACTGG 6 cut(s) 567, 831, 881, 1197, 1218, 1435
BseRI GAGGAG 1 cut(s) 230
BseSI GKGCMC 1 cut(s) 1143
BseX3I CGGCCG 1 cut(s) 352
BseXI GCAGC 7 cut(s) 209, 248, 398, 559, 1327, 1349, 1664
BseYI CCCAGC 1 cut(s) 1607
BsgI GTGCAG 2 cut(s) 638, 869
Bsh1236I CGCG 4 cut(s) 138, 357, 662, 815
Bsh1285I CGRYCG 1 cut(s) 355
BshNI GGYRCC 2 cut(s) 1196, 1431
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 3 cut(s) 375, 721, 963
BsiHKCI CYCGRG 3 cut(s) 229, 705, 1552
BsiSI CCGG 8 cut(s) 189, 230, 327, 474, 489, 567, 744, 1360
BslFI GGGAC 6 cut(s) 52, 1203, 1309, 1318, 1535, 1624
BslI CCNNNNNNNGG 7 cut(s) 274, 306, 802, 819, 977, 1366, 1697
BsmAI GTCTC 5 cut(s) 148, 731, 1062, 1412, 1708
BsmFI GGGAC 6 cut(s) 52, 1203, 1309, 1318, 1535, 1624
BsmI GAATGC 1 cut(s) 452
Bso31I GGTCTC 2 cut(s) 731, 1062
BsoBI CYCGRG 3 cut(s) 229, 705, 1552
Bsp1286I GDGCHC 4 cut(s) 375, 721, 963, 1143
Bsp143I GATC 4 cut(s) 321, 337, 376, 1687
Bsp19I CCATGG 4 cut(s) 294, 312, 1064, 1236
BspCNI CTCAG 5 cut(s) 544, 703, 975, 1213, 1609
BspFNI CGCG 4 cut(s) 138, 357, 662, 815
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 4 cut(s) 331, 881, 1198, 1433
BspPI GGATC 1 cut(s) 1682
BspT107I GGYRCC 2 cut(s) 1196, 1431
BspTNI GGTCTC 2 cut(s) 731, 1062
BsrBI CCGCTC 1 cut(s) 695
BsrDI GCAATG 2 cut(s) 1332, 1413
BsrFI RCCGGY 4 cut(s) 188, 473, 566, 1359
BsrI ACTGG 6 cut(s) 567, 831, 881, 1197, 1218, 1435
BssAI RCCGGY 4 cut(s) 188, 473, 566, 1359
BssMI GATC 4 cut(s) 321, 337, 376, 1687
BssNAI GTATAC 1 cut(s) 1477
BssNI GRCGYC 2 cut(s) 234, 918
BssT1I CCWWGG 7 cut(s) 294, 312, 423, 912, 1064, 1092, 1236
Bst1107I GTATAC 1 cut(s) 1477
Bst2UI CCWGG 5 cut(s) 71, 258, 368, 803, 947
Bst4CI ACNGT 4 cut(s) 34, 212, 247, 1481
Bst6I CTCTTC 1 cut(s) 945
BstACI GRCGYC 2 cut(s) 234, 918
BstC8I GCNNGC 4 cut(s) 190, 276, 280, 459
BstDEI CTNAG 5 cut(s) 552, 711, 962, 1221, 1617
BstDSI CCRYGG 7 cut(s) 268, 294, 312, 355, 813, 1064, 1236
BstEII GGTNACC 2 cut(s) 212, 1484
BstF5I GGATG 5 cut(s) 111, 523, 1060, 1246, 1294
BstFNI CGCG 4 cut(s) 138, 357, 662, 815
BstHHI GCGC 3 cut(s) 140, 853, 866
BstKTI GATC 4 cut(s) 324, 340, 379, 1690
BstMAI GTCTC 5 cut(s) 148, 731, 1062, 1412, 1708
BstMBI GATC 4 cut(s) 321, 337, 376, 1687
BstMCI CGRYCG 1 cut(s) 355
BstNI CCWGG 5 cut(s) 71, 258, 368, 803, 947
BstNSI RCATGY 1 cut(s) 97
BstPI GGTNACC 2 cut(s) 212, 1484
BstSCI CCNGG 8 cut(s) 69, 228, 229, 256, 366, 487, 801, 945
BstSLI GKGCMC 1 cut(s) 1143
BstUI CGCG 4 cut(s) 138, 357, 662, 815
BstV1I GCAGC 7 cut(s) 209, 248, 398, 559, 1327, 1349, 1664
BstV2I GAAGAC 1 cut(s) 1450
BstXI CCANNNNNNTGG 3 cut(s) 70, 295, 906
BstZ17I GTATAC 1 cut(s) 1477
BstZI CGGCCG 1 cut(s) 352
Bsu36I CCTNAGG 1 cut(s) 1617
BtgI CCRYGG 7 cut(s) 268, 294, 312, 355, 813, 1064, 1236
BtsCI GGATG 5 cut(s) 111, 523, 1060, 1246, 1294
BtsI GCAGTG 1 cut(s) 647
BtsIMutI CAGTG 4 cut(s) 647, 824, 1408, 1571
Cac8I GCNNGC 4 cut(s) 190, 276, 280, 459
CaiI CAGNNNCTG 2 cut(s) 257, 1343
CciI TCATGA 1 cut(s) 318
CfoI GCGC 3 cut(s) 140, 853, 866
Cfr10I RCCGGY 4 cut(s) 188, 473, 566, 1359
Cfr42I CCGCGG 2 cut(s) 358, 816
Cfr9I CCCGGG 1 cut(s) 229
CseI GACGC 2 cut(s) 223, 253
Csp6I GTAC 2 cut(s) 1197, 1432
CspCI CAANNNNNGTGG 4 cut(s) 1296, 1331, 1450, 1485
CviAII CATG 7 cut(s) 94, 295, 313, 319, 470, 1065, 1237
CviQI GTAC 2 cut(s) 1197, 1432
DdeI CTNAG 5 cut(s) 552, 711, 962, 1221, 1617
DpnI GATC 4 cut(s) 323, 339, 378, 1689
DpnII GATC 4 cut(s) 321, 337, 376, 1687
EaeI YGGCCR 5 cut(s) 297, 352, 564, 828, 1246
EagI CGGCCG 1 cut(s) 352
Eam1104I CTCTTC 1 cut(s) 945
EarI CTCTTC 1 cut(s) 945
EciI GGCGGA 3 cut(s) 20, 175, 193
Ecl136II GAGCTC 2 cut(s) 373, 961
EclXI CGGCCG 1 cut(s) 352
Eco130I CCWWGG 7 cut(s) 294, 312, 423, 912, 1064, 1092, 1236
Eco147I AGGCCT 1 cut(s) 670
Eco24I GRGCYC 2 cut(s) 375, 963
Eco31I GGTCTC 2 cut(s) 731, 1062
Eco47I GGWCC 2 cut(s) 909, 1278
Eco52I CGGCCG 1 cut(s) 352
Eco53kI GAGCTC 2 cut(s) 373, 961
Eco81I CCTNAGG 1 cut(s) 1617
Eco88I CYCGRG 3 cut(s) 229, 705, 1552
Eco91I GGTNACC 2 cut(s) 212, 1484
EcoICRI GAGCTC 2 cut(s) 373, 961
EcoO109I RGGNCCY 2 cut(s) 254, 556
EcoO65I GGTNACC 2 cut(s) 212, 1484
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 5 cut(s) 69, 256, 366, 801, 945
EcoT14I CCWWGG 7 cut(s) 294, 312, 423, 912, 1064, 1092, 1236
EcoT38I GRGCYC 2 cut(s) 375, 963
ErhI CCWWGG 7 cut(s) 294, 312, 423, 912, 1064, 1092, 1236
FaeI CATG 7 cut(s) 97, 298, 316, 322, 473, 1068, 1240
FalI AAGNNNNNCTT 2 cut(s) 1587, 1619
FaqI GGGAC 6 cut(s) 52, 1203, 1309, 1318, 1535, 1624
FatI CATG 7 cut(s) 93, 294, 312, 318, 469, 1064, 1236
FauI CCCGC 2 cut(s) 271, 1091
FbaI TGATCA 1 cut(s) 337
FblI GTMKAC 2 cut(s) 87, 1476
FokI GGATG 5 cut(s) 98, 530, 1047, 1253, 1301
FriOI GRGCYC 2 cut(s) 375, 963
FspBI CTAG 2 cut(s) 128, 731
GlaI GCGC 3 cut(s) 139, 852, 865
GsaI CCCAGC 1 cut(s) 1611
HapII CCGG 8 cut(s) 189, 230, 327, 474, 489, 567, 744, 1360
HgaI GACGC 2 cut(s) 223, 253
HhaI GCGC 3 cut(s) 140, 853, 866
Hin1I GRCGYC 2 cut(s) 234, 918
Hin1II CATG 7 cut(s) 97, 298, 316, 322, 473, 1068, 1240
Hin6I GCGC 3 cut(s) 138, 851, 864
HinP1I GCGC 3 cut(s) 138, 851, 864
HindIII AAGCTT 1 cut(s) 1581
HinfI GANTC 7 cut(s) 8, 159, 542, 582, 747, 1385, 1643
HpaII CCGG 8 cut(s) 189, 230, 327, 474, 489, 567, 744, 1360
HphI GGTGA 5 cut(s) 112, 235, 905, 1001, 1496
Hpy166II GTNNAC 3 cut(s) 88, 268, 1477
Hpy188III TCNNGA 5 cut(s) 319, 1153, 1355, 1389, 1552
Hpy8I GTNNAC 3 cut(s) 88, 268, 1477
Hpy99I CGWCG 2 cut(s) 38, 517
HpyAV CCTTC 2 cut(s) 178, 1373
HpyCH4III ACNGT 4 cut(s) 34, 212, 247, 1481
HpyCH4IV ACGT 3 cut(s) 846, 918, 1101
HpyF3I CTNAG 5 cut(s) 552, 711, 962, 1221, 1617
HpySE526I ACGT 3 cut(s) 846, 918, 1101
Hsp92I GRCGYC 2 cut(s) 234, 918
Hsp92II CATG 7 cut(s) 97, 298, 316, 322, 473, 1068, 1240
HspAI GCGC 3 cut(s) 138, 851, 864
KpnI GGTACC 2 cut(s) 1200, 1435
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 337
KspI CCGCGG 2 cut(s) 358, 816
Kzo9I GATC 4 cut(s) 321, 337, 376, 1687
LmnI GCTCC 6 cut(s) 335, 370, 692, 885, 1210, 1540
Lsp1109I GCAGC 7 cut(s) 209, 248, 398, 559, 1327, 1349, 1664
LweI GCATC 4 cut(s) 120, 982, 1168, 1279
MaeI CTAG 2 cut(s) 128, 731
MaeII ACGT 3 cut(s) 846, 918, 1101
MaeIII GTNAC 6 cut(s) 118, 212, 822, 842, 1325, 1484
MalI GATC 4 cut(s) 323, 339, 378, 1689
MbiI CCGCTC 1 cut(s) 695
MboI GATC 4 cut(s) 321, 337, 376, 1687
MboII GAAGA 3 cut(s) 962, 1450, 1503
MfeI CAATTG 1 cut(s) 1121
MhlI GDGCHC 4 cut(s) 375, 721, 963, 1143
MlsI TGGCCA 2 cut(s) 299, 830
MluNI TGGCCA 2 cut(s) 299, 830
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 2 cut(s) 312, 495
Mox20I TGGCCA 2 cut(s) 299, 830
MroNI GCCGGC 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 347
MscI TGGCCA 2 cut(s) 299, 830
MseI TTAA 5 cut(s) 417, 432, 873, 1029, 1146
MslI CAYNNNNRTG 3 cut(s) 317, 891, 904
Msp20I TGGCCA 2 cut(s) 299, 830
MspA1I CMGCKG 5 cut(s) 55, 357, 815, 1340, 1667
MspI CCGG 8 cut(s) 189, 230, 327, 474, 489, 567, 744, 1360
MspR9I CCNGG 8 cut(s) 71, 230, 231, 258, 368, 489, 803, 947
MunI CAATTG 1 cut(s) 1121
Mva1269I GAATGC 1 cut(s) 452
MvaI CCWGG 5 cut(s) 71, 258, 368, 803, 947
MvnI CGCG 4 cut(s) 138, 357, 662, 815
NaeI GCCGGC 1 cut(s) 190
NciI CCSGG 3 cut(s) 230, 231, 489
NcoI CCATGG 4 cut(s) 294, 312, 1064, 1236
NdeII GATC 4 cut(s) 321, 337, 376, 1687
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 7 cut(s) 97, 298, 316, 322, 473, 1068, 1240
NlaIV GGNNCC 4 cut(s) 331, 881, 1198, 1433
NmeAIII GCCGAG 2 cut(s) 601, 1274
NmuCI GTSAC 4 cut(s) 118, 822, 842, 1484
NspI RCATGY 1 cut(s) 97
OliI CACNNNNGTG 2 cut(s) 891, 904
PaeR7I CTCGAG 1 cut(s) 705
PagI TCATGA 1 cut(s) 318
PceI AGGCCT 1 cut(s) 670
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 441
PctI GAATGC 1 cut(s) 452
PdiI GCCGGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 6 cut(s) 159, 542, 582, 747, 1385, 1643
PflMI CCANNNNNTGG 3 cut(s) 274, 306, 802
PfoI TCCNGGA 1 cut(s) 366
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PsiI TTATAA 1 cut(s) 1673
Psp124BI GAGCTC 2 cut(s) 375, 963
Psp1406I AACGTT 1 cut(s) 1101
Psp6I CCWGG 5 cut(s) 69, 256, 366, 801, 945
PspEI GGTNACC 2 cut(s) 212, 1484
PspFI CCCAGC 1 cut(s) 1607
PspGI CCWGG 5 cut(s) 69, 256, 366, 801, 945
PspN4I GGNNCC 4 cut(s) 331, 881, 1198, 1433
PspXI VCTCGAGB 1 cut(s) 705
PstNI CAGNNNCTG 2 cut(s) 257, 1343
PvuII CAGCTG 3 cut(s) 55, 1340, 1667
RsaI GTAC 2 cut(s) 1198, 1433
RsaNI GTAC 2 cut(s) 1197, 1432
RseI CAYNNNNRTG 3 cut(s) 317, 891, 904
SacI GAGCTC 2 cut(s) 375, 963
SacII CCGCGG 2 cut(s) 358, 816
SaqAI TTAA 5 cut(s) 417, 432, 873, 1029, 1146
Sau3AI GATC 4 cut(s) 321, 337, 376, 1687
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 8 cut(s) 71, 230, 231, 258, 368, 489, 803, 947
SduI GDGCHC 4 cut(s) 375, 721, 963, 1143
SfaNI GCATC 4 cut(s) 120, 982, 1168, 1279
Sfr274I CTCGAG 1 cut(s) 705
Sfr303I CCGCGG 2 cut(s) 358, 816
SgrBI CCGCGG 2 cut(s) 358, 816
SinI GGWCC 2 cut(s) 909, 1278
SlaI CTCGAG 1 cut(s) 705
SmaI CCCGGG 1 cut(s) 231
SmiMI CAYNNNNRTG 3 cut(s) 317, 891, 904
SmlI CTYRAG 2 cut(s) 705, 1625
SmoI CTYRAG 2 cut(s) 705, 1625
SrfI GCCCGGGC 1 cut(s) 231
SseBI AGGCCT 1 cut(s) 670
SspMI CTAG 2 cut(s) 128, 731
SstI GAGCTC 2 cut(s) 375, 963
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 8 cut(s) 69, 228, 229, 256, 366, 487, 801, 945
StyI CCWWGG 7 cut(s) 294, 312, 423, 912, 1064, 1092, 1236
TaaI ACNGT 4 cut(s) 34, 212, 247, 1481
TaiI ACGT 3 cut(s) 849, 921, 1104
TaqI TCGA 4 cut(s) 375, 655, 706, 1071
TaqII GACCGA 1 cut(s) 1503
TauI GCSGC 8 cut(s) 228, 357, 360, 644, 698, 809, 818, 1562
TfiI GAWTC 6 cut(s) 159, 542, 582, 747, 1385, 1643
Tru1I TTAA 5 cut(s) 417, 432, 873, 1029, 1146
Tru9I TTAA 5 cut(s) 417, 432, 873, 1029, 1146
TscAI CASTG 4 cut(s) 654, 831, 1408, 1578
TseFI GTSAC 4 cut(s) 118, 822, 842, 1484
TseI GCWGC 7 cut(s) 197, 261, 386, 572, 1337, 1340, 1652
Tsp45I GTSAC 4 cut(s) 118, 822, 842, 1484
TspDTI ATGAA 3 cut(s) 916, 1178, 1518
TspMI CCCGGG 1 cut(s) 229
TspRI CASTG 4 cut(s) 654, 831, 1408, 1578
Van91I CCANNNNNTGG 3 cut(s) 274, 306, 802
VpaK11BI GGWCC 2 cut(s) 909, 1278
XapI RAATTY 1 cut(s) 622
XceI RCATGY 1 cut(s) 97
XcmI CCANNNNNNNNNTGG 1 cut(s) 1003
XhoI CTCGAG 1 cut(s) 705
XmaI CCCGGG 1 cut(s) 229
XmiI GTMKAC 2 cut(s) 87, 1476
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 2 cut(s) 128, 731
ZraI GACGTC 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.