FvH4_1g29570

Serine hydrolase (FSH1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015528
Physical Location & Seq
Forward (+)
22738461 .. 22740812
2352 bp
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UTR
Exon/CDS
Intron
FvH4_1g29570.t1

Sequence Viewer

Length: 825 bp
ATGGAAAGCCAGAAAGACAAGAAGTTGAAGATTCTATGCCTCCATGGATTTAGAACCAGTGGCAGCTTCCTGCAAAAGCAACTTAGCAAATGGGATGCTTCTATTCTTTCTCAGTTTGACATGGAGTTTCCAGATGGGAAGTTCCCTGCTGGTGGAAAATCTGACATAGAAGGCATCTTCCCACCTCCTTATTTTGAGTGGTTCCAATTTGAAAAGGACTTTATGGAGTACACAAACTTGGAAGCATGCATCTCTTATTTATGTGACTACATAACAGGCAAAGGCCCCTTTGATGGTTTGCTTGGGTTCTCTCAGGGCGCGACACTCTGTGGACTCTTGTTAGGCTACCAAGCTCAGGGAAAACTGTTGAAGGAGCATCCAACTTTCAGATTTTTCATATCGATATCAGGAGCAAAGTTCCGACAGCCAAGCATCTGCGAGATTGCCTACAAACAAAAAATCAAGGCCAAATCTGTCCACTTTATAGGAGCCAAAGATTGGTTAAAACTACCCTCTGAGGAGCTTGCTTCTGAGTTTCACAACCCCCTCATCATAAGGCACCCACAAGGCCATACTGTCCCTAGGCTAGATGAAGTAGCCATCGAACAGTTACGTGAGTGGACTAAGACAATCATCCAATCCAATACTGGCACTACTACTACTGCTCCTCCTCCTTTGGATAGTAATCATGAACTGAAAAATGGAGAAGCCAAGGCTCCAGTAGTTGCAGTATCATGTAATAAGGTCCAAACAGGGCAGCTGGAGACAAATGGAGTAATTATCACAGAGAAAACTGGGAGAGAAGTCGAGGCAGTCCAACCATAA

Protein Analysis

275

Amino Acids

30.66

Weight (kDa)

6.66

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 7 - 198 4.5e-51 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015528)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29570
malus_domestica MD12G1099800.v1.1
prunus_persica Prupe.7G024500_v2.0.a1 Prupe.7G024500_v2.0.a1 Prupe.7G024500_v2.0.a1
pyrus_communis pycom12g09380
rosa_chinensis RchiOBHm_Chr3g0495211
rosa_laevigata RLG00000022638
rosa_multiflora Rmu_sc0002943.1_g000026
rosa_roxburghii Rroxscaffold_6G00390690
rosa_rugosa Rorug03G0270000
rosa_samantha Rh3AG319700 Rh3BG354800 Rh3CG351700 Rh3DG353800
rosa_wichuraiana Rw3G027920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 558
AccB7I CCANNNNNTGG 1 cut(s) 498
AccII CGCG 1 cut(s) 320
AdeI CACNNNGTG 1 cut(s) 329
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 4 cut(s) 152, 293, 355, 498
AgsI TTSAA 3 cut(s) 28, 212, 370
AluBI AGCT 4 cut(s) 66, 353, 523, 760
AluI AGCT 4 cut(s) 66, 353, 523, 760
Alw26I GTCTC 1 cut(s) 758
AoxI GGCC 3 cut(s) 283, 465, 568
ApeKI GCWGC 2 cut(s) 63, 757
AspA2I CCTAGG 1 cut(s) 581
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 2 cut(s) 284, 745
AvaII GGWCC 1 cut(s) 745
AvrII CCTAGG 1 cut(s) 581
BanI GGYRCC 1 cut(s) 558
BbvI GCAGC 2 cut(s) 75, 769
BccI CCATC 3 cut(s) 128, 287, 608
BcoDI GTCTC 1 cut(s) 758
BfaI CTAG 2 cut(s) 582, 587
BisI GCNGC 2 cut(s) 64, 758
BlnI CCTAGG 1 cut(s) 581
BlsI GCNGC 2 cut(s) 65, 759
Bme18I GGWCC 1 cut(s) 745
BmgT120I GGNCC 2 cut(s) 284, 745
BmiI GGNNCC 5 cut(s) 203, 286, 490, 560, 717
BmrI ACTGGG 1 cut(s) 804
BmsI GCATC 5 cut(s) 85, 183, 258, 385, 441
BmuI ACTGGG 1 cut(s) 804
BpmI CTGGAG 2 cut(s) 702, 782
Bpu10I CCTNAGC 1 cut(s) 354
Bsa29I ATCGAT 1 cut(s) 401
BsaAI YACGTR 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 684
BsaJI CCNNGG 3 cut(s) 43, 581, 711
BsaXI ACNNNNNCTCC 4 cut(s) 649, 652, 679, 682
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 293, 355, 498
Bse1I ACTGG 4 cut(s) 57, 652, 719, 799
Bse8I GATNNNNATC 1 cut(s) 684
BseCI ATCGAT 1 cut(s) 401
BseDI CCNNGG 3 cut(s) 43, 581, 711
BseGI GGATG 3 cut(s) 100, 376, 633
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 4 cut(s) 152, 293, 355, 498
BseMII CTCAG 5 cut(s) 125, 326, 368, 507, 522
BseNI ACTGG 4 cut(s) 57, 652, 719, 799
BseRI GAGGAG 3 cut(s) 533, 657, 660
BseXI GCAGC 2 cut(s) 75, 769
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 3 cut(s) 285, 467, 570
BshNI GGYRCC 1 cut(s) 558
BshVI ATCGAT 1 cut(s) 401
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 4 cut(s) 152, 293, 355, 498
BsmAI GTCTC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 563
BsnI GGCC 3 cut(s) 285, 467, 570
Bsp19I CCATGG 1 cut(s) 43
BspANI GGCC 3 cut(s) 285, 467, 570
BspCNI CTCAG 5 cut(s) 124, 325, 367, 508, 523
BspDI ATCGAT 1 cut(s) 401
BspFNI CGCG 1 cut(s) 320
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 5 cut(s) 203, 286, 490, 560, 717
BspT107I GGYRCC 1 cut(s) 558
BsrI ACTGG 4 cut(s) 57, 652, 719, 799
BssECI CCNNGG 3 cut(s) 43, 581, 711
BssT1I CCWWGG 3 cut(s) 43, 581, 711
Bst4CI ACNGT 3 cut(s) 366, 577, 609
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 2 cut(s) 247, 525
BstDEI CTNAG 7 cut(s) 83, 111, 312, 354, 516, 531, 624
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 3 cut(s) 100, 376, 633
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 1 cut(s) 320
BstMAI GTCTC 1 cut(s) 758
BstNSI RCATGY 1 cut(s) 249
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 75, 769
Bsu15I ATCGAT 1 cut(s) 401
BsuRI GGCC 3 cut(s) 285, 467, 570
BsuTUI ATCGAT 1 cut(s) 401
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 3 cut(s) 100, 376, 633
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 2 cut(s) 247, 525
CciI TCATGA 1 cut(s) 688
CfoI GCGC 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 284, 745
ClaI ATCGAT 1 cut(s) 401
Csp6I GTAC 1 cut(s) 229
CviAII CATG 5 cut(s) 44, 121, 246, 689, 735
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 7 cut(s) 83, 111, 312, 354, 516, 531, 624
DraIII CACNNNGTG 1 cut(s) 329
Eco130I CCWWGG 3 cut(s) 43, 581, 711
Eco32I GATATC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 745
EcoO109I RGGNCCY 1 cut(s) 284
EcoRV GATATC 1 cut(s) 405
EcoT14I CCWWGG 3 cut(s) 43, 581, 711
EcoT22I ATGCAT 1 cut(s) 251
ErhI CCWWGG 3 cut(s) 43, 581, 711
FaeI CATG 5 cut(s) 47, 124, 249, 692, 738
FaqI GGGAC 1 cut(s) 563
FatI CATG 5 cut(s) 43, 120, 245, 688, 734
Fnu4HI GCNGC 2 cut(s) 64, 758
FokI GGATG 3 cut(s) 107, 363, 620
Fsp4HI GCNGC 2 cut(s) 64, 758
FspBI CTAG 2 cut(s) 582, 587
GlaI GCGC 1 cut(s) 319
GluI GCNGC 2 cut(s) 64, 758
GsuI CTGGAG 2 cut(s) 702, 782
HaeIII GGCC 3 cut(s) 285, 467, 570
HhaI GCGC 1 cut(s) 320
Hin1II CATG 5 cut(s) 47, 124, 249, 692, 738
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HinfI GANTC 2 cut(s) 31, 333
Hpy166II GTNNAC 4 cut(s) 231, 332, 478, 621
Hpy188I TCNGA 5 cut(s) 163, 389, 422, 517, 532
Hpy188III TCNNGA 3 cut(s) 131, 408, 689
Hpy8I GTNNAC 4 cut(s) 231, 332, 478, 621
HpyAV CCTTC 2 cut(s) 164, 364
HpyCH4III ACNGT 3 cut(s) 366, 577, 609
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 3 cut(s) 73, 249, 728
HpyF3I CTNAG 7 cut(s) 83, 111, 312, 354, 516, 531, 624
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 5 cut(s) 47, 124, 249, 692, 738
HspAI GCGC 1 cut(s) 318
LmnI GCTCC 6 cut(s) 373, 410, 488, 520, 670, 721
Lsp1109I GCAGC 2 cut(s) 75, 769
LweI GCATC 5 cut(s) 85, 183, 258, 385, 441
MaeI CTAG 2 cut(s) 582, 587
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 2 cut(s) 263, 609
MboII GAAGA 2 cut(s) 40, 169
MluCI AATT 2 cut(s) 206, 777
MlyI GAGTC 1 cut(s) 327
MmeI TCCRAC 2 cut(s) 404, 445
MnlI CCTC 8 cut(s) 50, 195, 511, 523, 557, 678, 681, 802
Mph1103I ATGCAT 1 cut(s) 251
MseI TTAA 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 760
MvnI CGCG 1 cut(s) 320
NcoI CCATGG 1 cut(s) 43
NlaIII CATG 5 cut(s) 47, 124, 249, 692, 738
NlaIV GGNNCC 5 cut(s) 203, 286, 490, 560, 717
NmuCI GTSAC 1 cut(s) 263
NsiI ATGCAT 1 cut(s) 251
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PagI TCATGA 1 cut(s) 688
PfeI GAWTC 1 cut(s) 31
PflMI CCANNNNNTGG 1 cut(s) 498
PkrI GCNGC 2 cut(s) 65, 759
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
Ppu21I YACGTR 1 cut(s) 614
PspN4I GGNNCC 5 cut(s) 203, 286, 490, 560, 717
PspPI GGNCC 2 cut(s) 284, 745
PvuII CAGCTG 1 cut(s) 760
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 2 cut(s) 64, 758
Sau96I GGNCC 2 cut(s) 284, 745
SchI GAGTC 1 cut(s) 327
SetI ASST 7 cut(s) 68, 187, 355, 525, 616, 747, 762
SfaNI GCATC 5 cut(s) 85, 183, 258, 385, 441
SinI GGWCC 1 cut(s) 745
SphI GCATGC 1 cut(s) 249
Sse9I AATT 2 cut(s) 206, 777
SspMI CTAG 2 cut(s) 582, 587
StyI CCWWGG 3 cut(s) 43, 581, 711
TaaI ACNGT 3 cut(s) 366, 577, 609
TaiI ACGT 1 cut(s) 616
TaqI TCGA 3 cut(s) 401, 603, 807
TasI AATT 2 cut(s) 206, 777
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 2 cut(s) 63, 757
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 3 cut(s) 385, 606, 705
TspRI CASTG 1 cut(s) 64
Van91I CCANNNNNTGG 1 cut(s) 498
VpaK11BI GGWCC 1 cut(s) 745
XceI RCATGY 1 cut(s) 249
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
XmaJI CCTAGG 1 cut(s) 581
XspI CTAG 2 cut(s) 582, 587
Zsp2I ATGCAT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.