RLG00000022638

Serine hydrolase (FSH1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015528
Physical Location & Seq
Reverse (-)
5985332 .. 5987350
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022638

Sequence Viewer

Length: 672 bp
ATGGAAAGCCAGAAAGATAAGAAGATGAAGATTCTATGCCTCCATGGATTTAGAACCAGTGGGAGCTTCCTTCAAAAGCAAATTAGCAAATGGGATGCTTCTATCCTTTCTCAGTTTGACATGGAGTTTCCAGATGGGAAGTTTCCTGCTGGAGGAAAATCTGACATAGAAGGCATCTTCCCACCTCCTTATTTTGAGTGGTTCCAATTTGACAAGGACTTCACGGAGTACACAAACTTGGAAGCATGCATCTCTCATTTATGTGACTACATAACAAGCAAAGGCCCCTTTGATGGTTTGCTTGGTTTCTCTCAGGGTGCGACACTATCAGGACTCTTGTTAGGCTACCAAGCTCAGGGACAACTGTTGAAGGAGCATCCAGCTTTCAAGTTTTTCATATCAATATCAGGATCAAAGTTCCGAAAGCCAAGTATCTGTGAGATTGCCTATAAAGACAGAATCAAGGTCAAATCTGTCCACTTGATAGGAGACAAAGATTGGTTAAAACTACCTTCTGAGGAGCTTGCTTCTGAATTTCACAACCCCCTCATCATAAGGCACCCACAAGGGCATACTGTCCCAAGGCTAGATGAAGTAGCCACCGAAAAGTTACGTGAATGGACTAAGACAATCATCCAATCCAATACTGCTGGAGACAAGTGGAGTACTTAA

Protein Analysis

224

Amino Acids

25.36

Weight (kDa)

7.05

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 7 - 199 5.2e-53 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015528)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29570
malus_domestica MD12G1099800.v1.1
prunus_persica Prupe.7G024500_v2.0.a1 Prupe.7G024500_v2.0.a1 Prupe.7G024500_v2.0.a1
pyrus_communis pycom12g09380
rosa_chinensis RchiOBHm_Chr3g0495211
rosa_laevigata RLG00000022638
rosa_multiflora Rmu_sc0002943.1_g000026
rosa_roxburghii Rroxscaffold_6G00390690
rosa_rugosa Rorug03G0270000
rosa_samantha Rh3AG319700 Rh3BG354800 Rh3CG351700 Rh3DG353800
rosa_wichuraiana Rw3G027920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 558
AclWI GGATC 1 cut(s) 418
AcsI RAATTY 1 cut(s) 533
AfaI GTAC 2 cut(s) 230, 667
AfiI CCNNNNNNNGG 3 cut(s) 152, 293, 355
AgsI TTSAA 3 cut(s) 74, 370, 388
AluBI AGCT 4 cut(s) 66, 353, 383, 523
AluI AGCT 4 cut(s) 66, 353, 383, 523
Alw26I GTCTC 2 cut(s) 483, 648
AlwI GGATC 1 cut(s) 418
AoxI GGCC 1 cut(s) 283
ApoI RAATTY 1 cut(s) 533
AspS9I GGNCC 1 cut(s) 284
BanI GGYRCC 1 cut(s) 558
BccI CCATC 2 cut(s) 128, 287
BcoDI GTCTC 2 cut(s) 483, 648
BfaI CTAG 1 cut(s) 587
BmcAI AGTACT 1 cut(s) 667
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 3 cut(s) 203, 286, 560
BmsI GCATC 4 cut(s) 85, 183, 258, 385
BpmI CTGGAG 2 cut(s) 171, 672
Bpu10I CCTNAGC 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 614
BsaJI CCNNGG 2 cut(s) 43, 581
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 293, 355
Bse1I ACTGG 1 cut(s) 57
BseDI CCNNGG 2 cut(s) 43, 581
BseGI GGATG 3 cut(s) 100, 376, 633
BseLI CCNNNNNNNGG 3 cut(s) 152, 293, 355
BseMII CTCAG 4 cut(s) 125, 326, 368, 507
BseNI ACTGG 1 cut(s) 57
BseRI GAGGAG 1 cut(s) 533
BshFI GGCC 1 cut(s) 285
BshNI GGYRCC 1 cut(s) 558
BslFI GGGAC 2 cut(s) 372, 563
BslI CCNNNNNNNGG 3 cut(s) 152, 293, 355
BsmAI GTCTC 2 cut(s) 483, 648
BsmFI GGGAC 2 cut(s) 372, 563
BsnI GGCC 1 cut(s) 285
Bsp143I GATC 1 cut(s) 410
Bsp19I CCATGG 1 cut(s) 43
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 4 cut(s) 124, 325, 367, 508
BspLI GGNNCC 3 cut(s) 203, 286, 560
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 558
BsrI ACTGG 1 cut(s) 57
BssECI CCNNGG 2 cut(s) 43, 581
BssMI GATC 1 cut(s) 410
BssT1I CCWWGG 2 cut(s) 43, 581
Bst4CI ACNGT 2 cut(s) 366, 577
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 2 cut(s) 247, 525
BstDEI CTNAG 5 cut(s) 111, 312, 354, 516, 624
BstDSI CCRYGG 1 cut(s) 43
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 3 cut(s) 100, 376, 633
BstKTI GATC 1 cut(s) 413
BstMAI GTCTC 2 cut(s) 483, 648
BstMBI GATC 1 cut(s) 410
BstNSI RCATGY 1 cut(s) 249
BsuRI GGCC 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 3 cut(s) 100, 376, 633
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 2 cut(s) 247, 525
Cfr13I GGNCC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 229, 666
CviAII CATG 3 cut(s) 44, 121, 246
CviQI GTAC 2 cut(s) 229, 666
DdeI CTNAG 5 cut(s) 111, 312, 354, 516, 624
DpnI GATC 1 cut(s) 412
DpnII GATC 1 cut(s) 410
Eco130I CCWWGG 2 cut(s) 43, 581
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoO109I RGGNCCY 1 cut(s) 284
EcoT14I CCWWGG 2 cut(s) 43, 581
EcoT22I ATGCAT 1 cut(s) 251
ErhI CCWWGG 2 cut(s) 43, 581
FaeI CATG 3 cut(s) 47, 124, 249
FaqI GGGAC 2 cut(s) 372, 563
FatI CATG 3 cut(s) 43, 120, 245
FokI GGATG 3 cut(s) 107, 363, 620
FspBI CTAG 1 cut(s) 587
GsuI CTGGAG 2 cut(s) 171, 672
HaeIII GGCC 1 cut(s) 285
Hin1II CATG 3 cut(s) 47, 124, 249
HinfI GANTC 3 cut(s) 31, 333, 459
Hpy166II GTNNAC 2 cut(s) 231, 478
Hpy188I TCNGA 4 cut(s) 163, 422, 517, 532
Hpy188III TCNNGA 3 cut(s) 131, 330, 408
Hpy8I GTNNAC 2 cut(s) 231, 478
HpyAV CCTTC 4 cut(s) 80, 164, 364, 522
HpyCH4III ACNGT 2 cut(s) 366, 577
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 1 cut(s) 249
HpyF3I CTNAG 5 cut(s) 111, 312, 354, 516, 624
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 3 cut(s) 47, 124, 249
Kzo9I GATC 1 cut(s) 410
LmnI GCTCC 3 cut(s) 63, 373, 520
LweI GCATC 4 cut(s) 85, 183, 258, 385
MaeI CTAG 1 cut(s) 587
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 2 cut(s) 263, 609
MalI GATC 1 cut(s) 412
MboI GATC 1 cut(s) 410
MboII GAAGA 3 cut(s) 34, 40, 169
MluCI AATT 3 cut(s) 81, 206, 533
MlyI GAGTC 1 cut(s) 327
MnlI CCTC 5 cut(s) 50, 146, 195, 511, 557
Mph1103I ATGCAT 1 cut(s) 251
MseI TTAA 2 cut(s) 503, 670
MslI CAYNNNNRTG 1 cut(s) 261
NcoI CCATGG 1 cut(s) 43
NdeII GATC 1 cut(s) 410
NlaIII CATG 3 cut(s) 47, 124, 249
NlaIV GGNNCC 3 cut(s) 203, 286, 560
NmuCI GTSAC 1 cut(s) 263
NsiI ATGCAT 1 cut(s) 251
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PfeI GAWTC 2 cut(s) 31, 459
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
Ppu21I YACGTR 1 cut(s) 614
PspN4I GGNNCC 3 cut(s) 203, 286, 560
PspPI GGNCC 1 cut(s) 284
RsaI GTAC 2 cut(s) 230, 667
RsaNI GTAC 2 cut(s) 229, 666
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 2 cut(s) 503, 670
Sau3AI GATC 1 cut(s) 410
Sau96I GGNCC 1 cut(s) 284
ScaI AGTACT 1 cut(s) 667
SchI GAGTC 1 cut(s) 327
SetI ASST 8 cut(s) 68, 187, 355, 385, 468, 514, 525, 616
SfaNI GCATC 4 cut(s) 85, 183, 258, 385
SmiMI CAYNNNNRTG 1 cut(s) 261
SphI GCATGC 1 cut(s) 249
Sse9I AATT 3 cut(s) 81, 206, 533
SspMI CTAG 1 cut(s) 587
StyI CCWWGG 2 cut(s) 43, 581
TaaI ACNGT 2 cut(s) 366, 577
TaiI ACGT 1 cut(s) 616
TasI AATT 3 cut(s) 81, 206, 533
TatI WGTACW 2 cut(s) 228, 665
TfiI GAWTC 2 cut(s) 31, 459
Tru1I TTAA 2 cut(s) 503, 670
Tru9I TTAA 2 cut(s) 503, 670
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 3 cut(s) 41, 385, 606
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 1 cut(s) 64
XagI CCTNNNNNAGG 1 cut(s) 150
XapI RAATTY 1 cut(s) 533
XceI RCATGY 1 cut(s) 249
XspI CTAG 1 cut(s) 587
ZrmI AGTACT 1 cut(s) 667
Zsp2I ATGCAT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.