RchiOBHm_Chr3g0495211

Serine hydrolase (FSH1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0015528
Physical Location & Seq
Forward (+)
44590588 .. 44593430
2843 bp
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UTR
Exon/CDS
Intron
PRQ45767

Sequence Viewer

Length: 819 bp
ATGGAAAGGCAGAAAGATACGAAGATGAAGATTCTATGCCTCCATGGATTTAGAACCAGTGGGAGCTTCCTGCAAAAGCAAATTAGCAAATGGGATGCTTCTATCCTTTCTCAGTTTGACATGGAGTTTCCAGATGGGAAGTTTCCTGCTGGAGGAAAATCTGACATAGAAGGCATCTTCCCACCTCCTTATTTTGAGTGGTTCCAATTTGACAAGGACTTCATGGAGTACACAAACTTGGAAGCATGCATCTCTTATTTATGTGATTACATAACAAGCAAAGGCCCCTTTGATGGTTTGCTTGGTTTCTCTCAGGGTGCGACACTATCAGGACTCTTGTTAGGCTACCAAGCTCAGGGAAAATTGTTGAAGGAGCATCCAGCTTTCAAATTTTTCATATCGATATCAGGATCAAAGTTCCGAAAGCCAAGTATCTGTGAGATTGCCTACAAAGACAGAATCAAGGTCAAATCTGTCCACTTGATAGGAGACAAAGATTGGTTAAAACTACCTTCTGAGGAGCTTGCTTCTGAATTTCACAACCCCCTCATCATAAGGCACCCACAAGGGCATACTGTCCCAAGGCTAGATGAAGTAGCCACCGAACAATTACGTGAGTGGACTAAGACAATCATCCAATCCAATACTGGTACTTCTCCTCCTCCTCCTTCAGATAGTAATCATGAACTGAAAAATGGAGAAGCCAAAAGTCCAGTAGTTGCAGCATCATGTAATAAGGTACAAACCGGGCAGCTGGGGACAAGTGGAGTACTTAACACAGAGAAAACAGAGAGAGAAGTCGAGGCAGTCCAAGCATAA

Protein Analysis

272

Amino Acids

30.44

Weight (kDa)

6.4

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 7 - 199 9.1e-53 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015528)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29570
malus_domestica MD12G1099800.v1.1
prunus_persica Prupe.7G024500_v2.0.a1 Prupe.7G024500_v2.0.a1 Prupe.7G024500_v2.0.a1
pyrus_communis pycom12g09380
rosa_chinensis RchiOBHm_Chr3g0495211
rosa_laevigata RLG00000022638
rosa_multiflora Rmu_sc0002943.1_g000026
rosa_roxburghii Rroxscaffold_6G00390690
rosa_rugosa Rorug03G0270000
rosa_samantha Rh3AG319700 Rh3BG354800 Rh3CG351700 Rh3DG353800
rosa_wichuraiana Rw3G027920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 558
AclWI GGATC 1 cut(s) 418
AcsI RAATTY 2 cut(s) 389, 533
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 4 cut(s) 230, 652, 741, 771
AfiI CCNNNNNNNGG 3 cut(s) 152, 293, 355
AgsI TTSAA 2 cut(s) 370, 388
AluBI AGCT 5 cut(s) 66, 353, 383, 523, 754
AluI AGCT 5 cut(s) 66, 353, 383, 523, 754
Alw26I GTCTC 1 cut(s) 483
AlwI GGATC 1 cut(s) 418
AoxI GGCC 1 cut(s) 283
ApeKI GCWGC 2 cut(s) 722, 751
ApoI RAATTY 2 cut(s) 389, 533
AspS9I GGNCC 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 748
BanI GGYRCC 1 cut(s) 558
BbvI GCAGC 2 cut(s) 734, 763
BccI CCATC 2 cut(s) 128, 287
BcnI CCSGG 1 cut(s) 748
BcoDI GTCTC 1 cut(s) 483
BfaI CTAG 1 cut(s) 587
BisI GCNGC 2 cut(s) 723, 752
BlsI GCNGC 2 cut(s) 724, 753
BmcAI AGTACT 1 cut(s) 771
Bme1390I CCNGG 1 cut(s) 748
BmgT120I GGNCC 1 cut(s) 284
BmiI GGNNCC 3 cut(s) 203, 286, 560
BmrFI CCNGG 1 cut(s) 748
BmsI GCATC 5 cut(s) 85, 183, 258, 385, 734
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 354
BpuMI CCSGG 1 cut(s) 748
Bsa29I ATCGAT 1 cut(s) 401
BsaAI YACGTR 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 678
BsaJI CCNNGG 2 cut(s) 43, 581
BsaXI ACNNNNNCTCC 2 cut(s) 643, 673
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 293, 355
Bse1I ACTGG 3 cut(s) 57, 652, 713
Bse8I GATNNNNATC 1 cut(s) 678
BseCI ATCGAT 1 cut(s) 401
BseDI CCNNGG 2 cut(s) 43, 581
BseGI GGATG 3 cut(s) 100, 376, 633
BseJI GATNNNNATC 1 cut(s) 678
BseLI CCNNNNNNNGG 3 cut(s) 152, 293, 355
BseMII CTCAG 4 cut(s) 125, 326, 368, 507
BseNI ACTGG 3 cut(s) 57, 652, 713
BseRI GAGGAG 4 cut(s) 533, 648, 651, 654
BseXI GCAGC 2 cut(s) 734, 763
BseYI CCCAGC 1 cut(s) 754
BshFI GGCC 1 cut(s) 285
BshNI GGYRCC 1 cut(s) 558
BshVI ATCGAT 1 cut(s) 401
BsiSI CCGG 1 cut(s) 747
BslFI GGGAC 2 cut(s) 563, 772
BslI CCNNNNNNNGG 3 cut(s) 152, 293, 355
BsmAI GTCTC 1 cut(s) 483
BsmFI GGGAC 2 cut(s) 563, 772
BsnI GGCC 1 cut(s) 285
Bsp143I GATC 1 cut(s) 410
Bsp19I CCATGG 1 cut(s) 43
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 4 cut(s) 124, 325, 367, 508
BspDI ATCGAT 1 cut(s) 401
BspHI TCATGA 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 203, 286, 560
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 558
BsrI ACTGG 3 cut(s) 57, 652, 713
BssECI CCNNGG 2 cut(s) 43, 581
BssMI GATC 1 cut(s) 410
BssT1I CCWWGG 2 cut(s) 43, 581
Bst4CI ACNGT 1 cut(s) 577
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 2 cut(s) 247, 525
BstDEI CTNAG 5 cut(s) 111, 312, 354, 516, 624
BstDSI CCRYGG 1 cut(s) 43
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 3 cut(s) 100, 376, 633
BstKTI GATC 1 cut(s) 413
BstMAI GTCTC 1 cut(s) 483
BstMBI GATC 1 cut(s) 410
BstMWI GCNNNNNNNGC 1 cut(s) 812
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 746
BstV1I GCAGC 2 cut(s) 734, 763
Bsu15I ATCGAT 1 cut(s) 401
BsuRI GGCC 1 cut(s) 285
BsuTUI ATCGAT 1 cut(s) 401
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 3 cut(s) 100, 376, 633
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 2 cut(s) 247, 525
CciI TCATGA 1 cut(s) 682
Cfr13I GGNCC 1 cut(s) 284
ClaI ATCGAT 1 cut(s) 401
Csp6I GTAC 4 cut(s) 229, 651, 740, 770
CviAII CATG 6 cut(s) 44, 121, 223, 246, 683, 729
CviQI GTAC 4 cut(s) 229, 651, 740, 770
DdeI CTNAG 5 cut(s) 111, 312, 354, 516, 624
DpnI GATC 1 cut(s) 412
DpnII GATC 1 cut(s) 410
Eco130I CCWWGG 2 cut(s) 43, 581
Eco32I GATATC 1 cut(s) 405
Eco57I CTGAAG 1 cut(s) 654
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoO109I RGGNCCY 1 cut(s) 284
EcoRV GATATC 1 cut(s) 405
EcoT14I CCWWGG 2 cut(s) 43, 581
EcoT22I ATGCAT 1 cut(s) 251
ErhI CCWWGG 2 cut(s) 43, 581
FaeI CATG 6 cut(s) 47, 124, 226, 249, 686, 732
FaqI GGGAC 2 cut(s) 563, 772
FatI CATG 6 cut(s) 43, 120, 222, 245, 682, 728
Fnu4HI GCNGC 2 cut(s) 723, 752
FokI GGATG 3 cut(s) 107, 363, 620
Fsp4HI GCNGC 2 cut(s) 723, 752
FspBI CTAG 1 cut(s) 587
GluI GCNGC 2 cut(s) 723, 752
GsaI CCCAGC 1 cut(s) 758
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 1 cut(s) 285
HapII CCGG 1 cut(s) 747
Hin1II CATG 6 cut(s) 47, 124, 226, 249, 686, 732
HinfI GANTC 3 cut(s) 31, 333, 459
HpaII CCGG 1 cut(s) 747
Hpy166II GTNNAC 3 cut(s) 231, 478, 621
Hpy188I TCNGA 5 cut(s) 163, 422, 517, 532, 673
Hpy188III TCNNGA 4 cut(s) 131, 330, 408, 683
Hpy8I GTNNAC 3 cut(s) 231, 478, 621
HpyAV CCTTC 4 cut(s) 164, 364, 522, 678
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 3 cut(s) 73, 249, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 812
HpyF3I CTNAG 5 cut(s) 111, 312, 354, 516, 624
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 6 cut(s) 47, 124, 226, 249, 686, 732
Kzo9I GATC 1 cut(s) 410
LmnI GCTCC 3 cut(s) 63, 373, 520
Lsp1109I GCAGC 2 cut(s) 734, 763
LweI GCATC 5 cut(s) 85, 183, 258, 385, 734
MaeI CTAG 1 cut(s) 587
MaeII ACGT 1 cut(s) 613
MalI GATC 1 cut(s) 412
MboI GATC 1 cut(s) 410
MboII GAAGA 3 cut(s) 34, 40, 169
MluCI AATT 6 cut(s) 81, 206, 362, 389, 533, 608
MlyI GAGTC 1 cut(s) 327
MnlI CCTC 9 cut(s) 50, 146, 195, 511, 557, 669, 672, 675, 796
Mph1103I ATGCAT 1 cut(s) 251
MseI TTAA 2 cut(s) 503, 774
MspA1I CMGCKG 1 cut(s) 754
MspI CCGG 1 cut(s) 747
MspR9I CCNGG 1 cut(s) 748
MwoI GCNNNNNNNGC 1 cut(s) 812
NciI CCSGG 1 cut(s) 748
NcoI CCATGG 1 cut(s) 43
NdeII GATC 1 cut(s) 410
NlaIII CATG 6 cut(s) 47, 124, 226, 249, 686, 732
NlaIV GGNNCC 3 cut(s) 203, 286, 560
NsiI ATGCAT 1 cut(s) 251
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PagI TCATGA 1 cut(s) 682
PfeI GAWTC 2 cut(s) 31, 459
PkrI GCNGC 2 cut(s) 724, 753
PleI GAGTC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 327
Ppu21I YACGTR 1 cut(s) 614
PspFI CCCAGC 1 cut(s) 754
PspN4I GGNNCC 3 cut(s) 203, 286, 560
PspPI GGNCC 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 754
RsaI GTAC 4 cut(s) 230, 652, 741, 771
RsaNI GTAC 4 cut(s) 229, 651, 740, 770
SaqAI TTAA 2 cut(s) 503, 774
SatI GCNGC 2 cut(s) 723, 752
Sau3AI GATC 1 cut(s) 410
Sau96I GGNCC 1 cut(s) 284
ScaI AGTACT 1 cut(s) 771
SchI GAGTC 1 cut(s) 327
ScrFI CCNGG 1 cut(s) 748
SfaNI GCATC 5 cut(s) 85, 183, 258, 385, 734
SphI GCATGC 1 cut(s) 249
Sse9I AATT 6 cut(s) 81, 206, 362, 389, 533, 608
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 1 cut(s) 746
StyI CCWWGG 2 cut(s) 43, 581
TaaI ACNGT 1 cut(s) 577
TaiI ACGT 1 cut(s) 616
TaqI TCGA 2 cut(s) 401, 801
TasI AATT 6 cut(s) 81, 206, 362, 389, 533, 608
TatI WGTACW 2 cut(s) 228, 769
TfiI GAWTC 2 cut(s) 31, 459
Tru1I TTAA 2 cut(s) 503, 774
Tru9I TTAA 2 cut(s) 503, 774
TscAI CASTG 1 cut(s) 64
TseI GCWGC 2 cut(s) 722, 751
TspDTI ATGAA 5 cut(s) 41, 211, 385, 606, 699
TspRI CASTG 1 cut(s) 64
XagI CCTNNNNNAGG 1 cut(s) 150
XapI RAATTY 2 cut(s) 389, 533
XceI RCATGY 1 cut(s) 249
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
XspI CTAG 1 cut(s) 587
ZrmI AGTACT 1 cut(s) 771
Zsp2I ATGCAT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.