Rroxscaffold_6G00390690

Serine hydrolase (FSH1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015528
Physical Location & Seq
Reverse (-)
6262903 .. 6265111
2209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00390690.1

Sequence Viewer

Length: 726 bp
ATGGAAAGCCAGAAAGATAAGAAGATGAAGATTCTATGCCTCCATGGATTTAGAACCAGTGGGAGCTTCCTGCAAAAGCAAATTAGCAAATGGGATGCTTCTATCCTTTCTCAGTTTGATATGGACTTCACGGAGTACACAAACTTGGAAGCATGCATCTCTTATTTATGTGATTACATAACAAGCAAAGGCCCCTTTGATGGTTTGCTTGGTTTCTCTCAGGGTGCGACACTATCAGGACTCTTGTTAGGCTACCAAGCTCAGGGAAAACTGTTGAAGGAGCATCCAGCTTTCAAAATTTTCATATCGATATCAGGATCAAAGTTCCGAAAGCCAAGTATCTGTGAGATTGCCTACAAAGACAGAATCAAGGTCAAATCTGTCCACTTGATAGGAGACAAAGATTGGTTAAAACTACCTTCTGAGGAGCTTGCTTCTGAATTTCACAACCCCCTCATCATAAGGCACCCACAAGGGCATACCGTCCCAAGGCTAGATGAAGTAGCCACCGAACAGTTACGTGAGTGGACTAAGACAATCATCCAATCCAATACTGGTACTACTCCTCCTCCTCCTTTAGATAGTAATCATGAACTGAGAAATGGAGAAGCCAAAAGTCCAGTAGTTGCAGCATCATGTAATAAGGTTCAAACCGGCCAGCTGGAGACAAGTGGAGTACTTAACACAGAGAAAACAGAGAGAGAAGTCGAGGCAGTCCAAGCATAA

Protein Analysis

241

Amino Acids

26.9

Weight (kDa)

7.05

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 41 - 168 2.3e-34 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015528)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29570
malus_domestica MD12G1099800.v1.1
prunus_persica Prupe.7G024500_v2.0.a1 Prupe.7G024500_v2.0.a1 Prupe.7G024500_v2.0.a1
pyrus_communis pycom12g09380
rosa_chinensis RchiOBHm_Chr3g0495211
rosa_laevigata RLG00000022638
rosa_multiflora Rmu_sc0002943.1_g000026
rosa_roxburghii Rroxscaffold_6G00390690
rosa_rugosa Rorug03G0270000
rosa_samantha Rh3AG319700 Rh3BG354800 Rh3CG351700 Rh3DG353800
rosa_wichuraiana Rw3G027920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 465
AclWI GGATC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 655
AcsI RAATTY 2 cut(s) 297, 440
AfaI GTAC 3 cut(s) 137, 559, 678
AfiI CCNNNNNNNGG 3 cut(s) 200, 262, 489
AgsI TTSAA 3 cut(s) 277, 295, 650
AluBI AGCT 5 cut(s) 66, 260, 290, 430, 661
AluI AGCT 5 cut(s) 66, 260, 290, 430, 661
Alw26I GTCTC 2 cut(s) 390, 659
AlwI GGATC 1 cut(s) 325
AoxI GGCC 2 cut(s) 190, 655
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 2 cut(s) 297, 440
AspS9I GGNCC 1 cut(s) 191
BanI GGYRCC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 641
BccI CCATC 1 cut(s) 194
BcoDI GTCTC 2 cut(s) 390, 659
BfaI CTAG 1 cut(s) 494
BisI GCNGC 1 cut(s) 630
BlsI GCNGC 1 cut(s) 631
BmcAI AGTACT 1 cut(s) 678
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 2 cut(s) 193, 467
BmsI GCATC 4 cut(s) 85, 165, 292, 641
BpmI CTGGAG 1 cut(s) 683
Bpu10I CCTNAGC 1 cut(s) 261
Bsa29I ATCGAT 1 cut(s) 308
BsaAI YACGTR 1 cut(s) 521
BsaBI GATNNNNATC 1 cut(s) 585
BsaJI CCNNGG 2 cut(s) 43, 488
BsaXI ACNNNNNCTCC 4 cut(s) 550, 553, 580, 583
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 262, 489
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 3 cut(s) 57, 559, 620
Bse8I GATNNNNATC 1 cut(s) 585
BseCI ATCGAT 1 cut(s) 308
BseDI CCNNGG 2 cut(s) 43, 488
BseGI GGATG 3 cut(s) 100, 283, 540
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 3 cut(s) 200, 262, 489
BseMII CTCAG 5 cut(s) 125, 233, 275, 414, 587
BseNI ACTGG 3 cut(s) 57, 559, 620
BseRI GAGGAG 4 cut(s) 440, 555, 558, 561
BseXI GCAGC 1 cut(s) 641
BshFI GGCC 2 cut(s) 192, 657
BshNI GGYRCC 1 cut(s) 465
BshVI ATCGAT 1 cut(s) 308
BsiSI CCGG 1 cut(s) 654
BslFI GGGAC 1 cut(s) 470
BslI CCNNNNNNNGG 3 cut(s) 200, 262, 489
BsmAI GTCTC 2 cut(s) 390, 659
BsmFI GGGAC 1 cut(s) 470
BsnI GGCC 2 cut(s) 192, 657
Bsp143I GATC 1 cut(s) 317
Bsp19I CCATGG 1 cut(s) 43
BspANI GGCC 2 cut(s) 192, 657
BspCNI CTCAG 5 cut(s) 124, 232, 274, 415, 588
BspDI ATCGAT 1 cut(s) 308
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 2 cut(s) 193, 467
BspPI GGATC 1 cut(s) 325
BspT107I GGYRCC 1 cut(s) 465
BsrFI RCCGGY 1 cut(s) 653
BsrI ACTGG 3 cut(s) 57, 559, 620
BssAI RCCGGY 1 cut(s) 653
BssECI CCNNGG 2 cut(s) 43, 488
BssMI GATC 1 cut(s) 317
BssT1I CCWWGG 2 cut(s) 43, 488
Bst4CI ACNGT 3 cut(s) 273, 484, 516
BstBAI YACGTR 1 cut(s) 521
BstC8I GCNNGC 3 cut(s) 154, 432, 659
BstDEI CTNAG 6 cut(s) 111, 219, 261, 423, 531, 596
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 3 cut(s) 100, 283, 540
BstKTI GATC 1 cut(s) 320
BstMAI GTCTC 2 cut(s) 390, 659
BstMBI GATC 1 cut(s) 317
BstMWI GCNNNNNNNGC 1 cut(s) 719
BstNSI RCATGY 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 641
Bsu15I ATCGAT 1 cut(s) 308
BsuRI GGCC 2 cut(s) 192, 657
BsuTUI ATCGAT 1 cut(s) 308
BtgI CCRYGG 1 cut(s) 43
BtsCI GGATG 3 cut(s) 100, 283, 540
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 3 cut(s) 154, 432, 659
CciI TCATGA 1 cut(s) 589
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 1 cut(s) 191
ClaI ATCGAT 1 cut(s) 308
Csp6I GTAC 3 cut(s) 136, 558, 677
CviAII CATG 4 cut(s) 44, 153, 590, 636
CviQI GTAC 3 cut(s) 136, 558, 677
DdeI CTNAG 6 cut(s) 111, 219, 261, 423, 531, 596
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
EaeI YGGCCR 1 cut(s) 655
Eco130I CCWWGG 2 cut(s) 43, 488
Eco32I GATATC 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 191
EcoRV GATATC 1 cut(s) 312
EcoT14I CCWWGG 2 cut(s) 43, 488
EcoT22I ATGCAT 1 cut(s) 158
ErhI CCWWGG 2 cut(s) 43, 488
FaeI CATG 4 cut(s) 47, 156, 593, 639
FaqI GGGAC 1 cut(s) 470
FatI CATG 4 cut(s) 43, 152, 589, 635
Fnu4HI GCNGC 1 cut(s) 630
FokI GGATG 3 cut(s) 107, 270, 527
Fsp4HI GCNGC 1 cut(s) 630
FspBI CTAG 1 cut(s) 494
GluI GCNGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 2 cut(s) 192, 657
HapII CCGG 1 cut(s) 654
Hin1II CATG 4 cut(s) 47, 156, 593, 639
HinfI GANTC 3 cut(s) 31, 240, 366
HpaII CCGG 1 cut(s) 654
Hpy166II GTNNAC 3 cut(s) 138, 385, 528
Hpy188I TCNGA 3 cut(s) 329, 424, 439
Hpy188III TCNNGA 3 cut(s) 237, 315, 590
Hpy8I GTNNAC 3 cut(s) 138, 385, 528
HpyAV CCTTC 2 cut(s) 271, 429
HpyCH4III ACNGT 3 cut(s) 273, 484, 516
HpyCH4IV ACGT 1 cut(s) 520
HpyCH4V TGCA 3 cut(s) 73, 156, 629
HpyF10VI GCNNNNNNNGC 1 cut(s) 719
HpyF3I CTNAG 6 cut(s) 111, 219, 261, 423, 531, 596
HpySE526I ACGT 1 cut(s) 520
Hsp92II CATG 4 cut(s) 47, 156, 593, 639
Kzo9I GATC 1 cut(s) 317
LmnI GCTCC 3 cut(s) 63, 280, 427
Lsp1109I GCAGC 1 cut(s) 641
LweI GCATC 4 cut(s) 85, 165, 292, 641
MaeI CTAG 1 cut(s) 494
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 1 cut(s) 516
MalI GATC 1 cut(s) 319
MboI GATC 1 cut(s) 317
MboII GAAGA 2 cut(s) 34, 40
MluCI AATT 3 cut(s) 81, 297, 440
MlyI GAGTC 1 cut(s) 234
MnlI CCTC 7 cut(s) 50, 418, 464, 576, 579, 582, 703
Mph1103I ATGCAT 1 cut(s) 158
MseI TTAA 2 cut(s) 410, 681
MspA1I CMGCKG 1 cut(s) 661
MspI CCGG 1 cut(s) 654
MwoI GCNNNNNNNGC 1 cut(s) 719
NcoI CCATGG 1 cut(s) 43
NdeII GATC 1 cut(s) 317
NlaIII CATG 4 cut(s) 47, 156, 593, 639
NlaIV GGNNCC 2 cut(s) 193, 467
NsiI ATGCAT 1 cut(s) 158
NspI RCATGY 1 cut(s) 156
PaeI GCATGC 1 cut(s) 156
PagI TCATGA 1 cut(s) 589
PfeI GAWTC 2 cut(s) 31, 366
PkrI GCNGC 1 cut(s) 631
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
Ppu21I YACGTR 1 cut(s) 521
PspN4I GGNNCC 2 cut(s) 193, 467
PspPI GGNCC 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 661
RsaI GTAC 3 cut(s) 137, 559, 678
RsaNI GTAC 3 cut(s) 136, 558, 677
SaqAI TTAA 2 cut(s) 410, 681
SatI GCNGC 1 cut(s) 630
Sau3AI GATC 1 cut(s) 317
Sau96I GGNCC 1 cut(s) 191
ScaI AGTACT 1 cut(s) 678
SchI GAGTC 1 cut(s) 234
SetI ASST 9 cut(s) 68, 262, 292, 375, 421, 432, 523, 648, 663
SfaNI GCATC 4 cut(s) 85, 165, 292, 641
SphI GCATGC 1 cut(s) 156
Sse9I AATT 3 cut(s) 81, 297, 440
SspMI CTAG 1 cut(s) 494
StyI CCWWGG 2 cut(s) 43, 488
TaaI ACNGT 3 cut(s) 273, 484, 516
TaiI ACGT 1 cut(s) 523
TaqI TCGA 2 cut(s) 308, 708
TasI AATT 3 cut(s) 81, 297, 440
TatI WGTACW 2 cut(s) 135, 676
TfiI GAWTC 2 cut(s) 31, 366
Tru1I TTAA 2 cut(s) 410, 681
Tru9I TTAA 2 cut(s) 410, 681
TscAI CASTG 1 cut(s) 64
TseI GCWGC 1 cut(s) 629
TspDTI ATGAA 4 cut(s) 41, 292, 513, 606
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 1 cut(s) 64
XapI RAATTY 2 cut(s) 297, 440
XceI RCATGY 1 cut(s) 156
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
XspI CTAG 1 cut(s) 494
ZrmI AGTACT 1 cut(s) 678
Zsp2I ATGCAT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.