FvH4_1g30270

Calmodulin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006992
Physical Location & Seq
Reverse (-)
23974311 .. 23976546
2236 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g30270.t1

Sequence Viewer

Length: 582 bp
ATGAGATGCCGGTGGACCACTACACCCCTCTTTGTTTTCATATATAAAGTCCTCCTCCACCTTCCAAAGTTATACTCTTCCATTTTGCTCTCTCTCTCTGATTTTCTTGCAAACCCCAAACCTCCTCCATCCATGGCCGATCAGCTCACCGACGACCAGATCTCTGAGTTCAAGGAGGCTTTCAGCCTCTTCGACAAGGACGGCGATGGCTGCATTACTACCAAGGAGCTTGGCACTGTTATGCGGTCCCTTGGGCAGAACCCAACCGAGGCTGAGCTTCAGGATATGATCAACGAGGTTGATGCTGATGGGAATGGTACTATTGATTTCCCAGAGTTCCTCAACCTGATGGCTCGTAAGATGAAGGACACTGATTCTGAGGAGGAGCTCAAGGAAGCGTTCCGGGTGTTTGATAAAGACCAGAATGGCTTCATTTCTGCTGCTGAGCTCCGTCATGTTATGACAAATCTGGGCGAGAAGCTGACAGATGAGGAAGTTGATGAGATGATCCGTGAGGCTGATGTGGATGGTGATGGACAGATCAACTATGAGGAGTTCGTCAAAGTCATGATGGCCAAGTGA

Protein Analysis

194

Amino Acids

21.93

Weight (kDa)

4.37

Isoelectric Point (pI)

27.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AIF-1 PF21008 46 - 120 2e-08 Allograft inflammatory factor 1
EF-hand_1 PF00036 56 - 84 5.7e-09 EF hand domain
EF-hand_7 PF13499 56 - 117 3.1e-17 EF-hand domain pair
EF-hand_6 PF13405 56 - 85 4.1e-09 EF-hand domain
EF-hand_9 PF14658 59 - 119 9.7e-10 EF-hand domain
EF-hand_8 PF13833 69 - 119 1.2e-10 EF-hand domain pair
EF-hand_1 PF00036 92 - 119 4.9e-08 EF hand domain
EF-hand_7 PF13499 127 - 191 1e-21 EF-hand domain pair
AIF-1 PF21008 128 - 193 5.1e-06 Allograft inflammatory factor 1
EF-hand_1 PF00036 129 - 156 3.8e-09 EF hand domain
EF-hand_6 PF13405 129 - 158 3e-09 EF-hand domain
EF-hand_5 PF13202 130 - 154 2e-06 EF hand
EF-hand_9 PF14658 132 - 192 5.7e-07 EF-hand domain
EF-hand_8 PF13833 142 - 190 1.7e-12 EF-hand domain pair
EF-hand_1 PF00036 165 - 192 5.5e-10 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 244
AclWI GGATC 1 cut(s) 502
AcoI YGGCCR 2 cut(s) 135, 573
AcuI CTGAAG 1 cut(s) 263
AfaI GTAC 1 cut(s) 319
AfiI CCNNNNNNNGG 1 cut(s) 268
AgsI TTSAA 1 cut(s) 172
AluBI AGCT 6 cut(s) 145, 229, 277, 388, 448, 481
AluI AGCT 6 cut(s) 145, 229, 277, 388, 448, 481
Alw21I GWGCWC 2 cut(s) 390, 450
AlwI GGATC 1 cut(s) 502
AoxI GGCC 2 cut(s) 135, 573
ApeKI GCWGC 2 cut(s) 210, 440
Asp700I GAANNNNTTC 1 cut(s) 428
AspS9I GGNCC 2 cut(s) 15, 246
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 2 cut(s) 139, 542
AvaII GGWCC 2 cut(s) 15, 246
BalI TGGCCA 1 cut(s) 575
BanII GRGCYC 2 cut(s) 390, 450
Bbv12I GWGCWC 2 cut(s) 390, 450
BbvI GCAGC 2 cut(s) 197, 427
BccI CCATC 7 cut(s) 136, 200, 302, 343, 521, 527, 565
BceAI ACGGC 1 cut(s) 217
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BclI TGATCA 1 cut(s) 288
BcnI CCSGG 1 cut(s) 404
BglII AGATCT 1 cut(s) 159
BisI GCNGC 2 cut(s) 211, 441
BlpI GCTNAGC 2 cut(s) 273, 444
BlsI GCNGC 2 cut(s) 212, 442
Bme1390I CCNGG 1 cut(s) 404
Bme18I GGWCC 2 cut(s) 15, 246
BmgT120I GGNCC 2 cut(s) 15, 246
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 404
BmsI GCATC 1 cut(s) 292
Bpu1102I GCTNAGC 2 cut(s) 273, 444
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 404
BsaJI CCNNGG 4 cut(s) 132, 222, 250, 267
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse118I RCCGGY 1 cut(s) 9
BseDI CCNNGG 4 cut(s) 132, 222, 250, 267
BseGI GGATG 2 cut(s) 128, 532
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 4 cut(s) 156, 264, 369, 435
BseRI GAGGAG 5 cut(s) 44, 114, 395, 398, 566
BseXI GCAGC 2 cut(s) 197, 427
BshFI GGCC 2 cut(s) 137, 575
BsiHKAI GWGCWC 2 cut(s) 390, 450
BsiSI CCGG 2 cut(s) 10, 403
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 232
BsnI GGCC 2 cut(s) 137, 575
Bsp1286I GDGCHC 2 cut(s) 390, 450
Bsp143I GATC 5 cut(s) 139, 159, 288, 507, 540
Bsp1720I GCTNAGC 2 cut(s) 273, 444
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 1 cut(s) 244
BspANI GGCC 2 cut(s) 137, 575
BspCNI CTCAG 4 cut(s) 157, 265, 370, 436
BspHI TCATGA 1 cut(s) 567
BspLI GGNNCC 1 cut(s) 248
BspPI GGATC 1 cut(s) 502
BsrFI RCCGGY 1 cut(s) 9
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 4 cut(s) 132, 222, 250, 267
BssMI GATC 5 cut(s) 139, 159, 288, 507, 540
BssT1I CCWWGG 3 cut(s) 132, 222, 250
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 2 cut(s) 82, 194
BstDEI CTNAG 4 cut(s) 165, 273, 378, 444
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 2 cut(s) 128, 532
BstKTI GATC 5 cut(s) 142, 162, 291, 510, 543
BstMBI GATC 5 cut(s) 139, 159, 288, 507, 540
BstMWI GCNNNNNNNGC 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 402
BstV1I GCAGC 2 cut(s) 197, 427
BstX2I RGATCY 1 cut(s) 159
BstYI RGATCY 1 cut(s) 159
BsuRI GGCC 2 cut(s) 137, 575
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 219
BtsCI GGATG 2 cut(s) 128, 532
BtsIMutI CAGTG 2 cut(s) 234, 369
CciI TCATGA 1 cut(s) 567
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 15, 246
Csp6I GTAC 1 cut(s) 318
CviAII CATG 3 cut(s) 133, 455, 568
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 4 cut(s) 165, 273, 378, 444
DpnI GATC 5 cut(s) 141, 161, 290, 509, 542
DpnII GATC 5 cut(s) 139, 159, 288, 507, 540
EaeI YGGCCR 2 cut(s) 135, 573
Eam1104I CTCTTC 2 cut(s) 82, 194
EarI CTCTTC 2 cut(s) 82, 194
Ecl136II GAGCTC 2 cut(s) 388, 448
Eco130I CCWWGG 3 cut(s) 132, 222, 250
Eco24I GRGCYC 2 cut(s) 390, 450
Eco47I GGWCC 2 cut(s) 15, 246
Eco53kI GAGCTC 2 cut(s) 388, 448
Eco57I CTGAAG 1 cut(s) 263
EcoICRI GAGCTC 2 cut(s) 388, 448
EcoT14I CCWWGG 3 cut(s) 132, 222, 250
EcoT38I GRGCYC 2 cut(s) 390, 450
ErhI CCWWGG 3 cut(s) 132, 222, 250
FaeI CATG 3 cut(s) 136, 458, 571
FaqI GGGAC 1 cut(s) 232
FatI CATG 3 cut(s) 132, 454, 567
FbaI TGATCA 1 cut(s) 288
Fnu4HI GCNGC 2 cut(s) 211, 441
FokI GGATG 2 cut(s) 115, 539
FriOI GRGCYC 2 cut(s) 390, 450
Fsp4HI GCNGC 2 cut(s) 211, 441
GluI GCNGC 2 cut(s) 211, 441
HaeIII GGCC 2 cut(s) 137, 575
HapII CCGG 2 cut(s) 10, 403
Hin1II CATG 3 cut(s) 136, 458, 571
HinfI GANTC 1 cut(s) 374
HpaII CCGG 2 cut(s) 10, 403
HphI GGTGA 2 cut(s) 139, 542
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 3 cut(s) 100, 166, 379
Hpy188III TCNNGA 2 cut(s) 281, 568
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 71, 358
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 2 cut(s) 110, 213
HpyF10VI GCNNNNNNNGC 1 cut(s) 210
HpyF3I CTNAG 4 cut(s) 165, 273, 378, 444
Hsp92II CATG 3 cut(s) 136, 458, 571
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 5 cut(s) 139, 159, 288, 507, 540
LmnI GCTCC 3 cut(s) 226, 385, 453
LpnPI CCDG 8 cut(s) 23, 170, 266, 345, 359, 416, 434, 455
Lsp1109I GCAGC 2 cut(s) 197, 427
LweI GCATC 1 cut(s) 292
MalI GATC 5 cut(s) 141, 161, 290, 509, 542
MboI GATC 5 cut(s) 139, 159, 288, 507, 540
MboII GAAGA 2 cut(s) 69, 181
MflI RGATCY 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 390, 450
MlsI TGGCCA 1 cut(s) 575
MluNI TGGCCA 1 cut(s) 575
Mox20I TGGCCA 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 428
MscI TGGCCA 1 cut(s) 575
MslI CAYNNNNRTG 1 cut(s) 239
Msp20I TGGCCA 1 cut(s) 575
MspI CCGG 2 cut(s) 10, 403
MspR9I CCNGG 1 cut(s) 404
MwoI GCNNNNNNNGC 1 cut(s) 210
NciI CCSGG 1 cut(s) 404
NcoI CCATGG 1 cut(s) 132
NdeII GATC 5 cut(s) 139, 159, 288, 507, 540
NlaIII CATG 3 cut(s) 136, 458, 571
NlaIV GGNNCC 1 cut(s) 248
PagI TCATGA 1 cut(s) 567
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 2 cut(s) 212, 442
Psp124BI GAGCTC 2 cut(s) 390, 450
PspN4I GGNNCC 1 cut(s) 248
PspPI GGNCC 2 cut(s) 15, 246
PsuI RGATCY 1 cut(s) 159
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 2 cut(s) 390, 450
SatI GCNGC 2 cut(s) 211, 441
Sau3AI GATC 5 cut(s) 139, 159, 288, 507, 540
Sau96I GGNCC 2 cut(s) 15, 246
ScrFI CCNGG 1 cut(s) 404
SduI GDGCHC 2 cut(s) 390, 450
SfaNI GCATC 1 cut(s) 292
SinI GGWCC 2 cut(s) 15, 246
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
SsiI CCGC 1 cut(s) 244
SstI GAGCTC 2 cut(s) 390, 450
StyD4I CCNGG 1 cut(s) 402
StyI CCWWGG 3 cut(s) 132, 222, 250
TaaI ACNGT 1 cut(s) 238
TaqI TCGA 1 cut(s) 192
TfiI GAWTC 1 cut(s) 374
TscAI CASTG 2 cut(s) 241, 376
TseI GCWGC 2 cut(s) 210, 440
TspDTI ATGAA 3 cut(s) 28, 377, 421
TspGWI ACGGA 2 cut(s) 440, 500
TspRI CASTG 2 cut(s) 241, 376
VpaK11BI GGWCC 2 cut(s) 15, 246
XmnI GAANNNNTTC 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.