Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0013945

Physical Location & Seq

Genomic Coordinates Forward (+)

4371418 .. 4374380

Gene Structure 2963 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g05310.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1806 bp

ATGGCCACGTCAGCATCCCAAACCCTCCGCACCTCCTTCCTCGGAAACCCTACTTCCTCCTCCTCGCTTTCTCTCTCCTCCAAGAACCCCCTCGCCTTCTCCCTCCCCTCCCGCCGCCGCCGCCTCGCCGCGCCCAGGCTCGTCGTGGTCCTCTCCGCCGCGAGACTCGACGCCAAGCCCACCGTCCTCGTCGCCGAGAAGCTCGGCGAGGCGGGGCTCGACCTCCTGAAGGAGTTCGCCAACGTGGACTGCTCGTATAACCTGAGCCCCGAGGAGCTCTGCACCAAGATCTCGCTCTGCGACGCCTTGATTGTCCGGAGCGGCACCAAGGTCAGCCGCGAGGTCTTCGAGTCCTCCGGCGGGAGGTTGAAGGTGGTTGGCCGAGCCGGCGTTGGGATTGATAATGTGGATCTGGGCGCCGCCACCGAGTTTGGCTGCTTGGTTGTTAATGCTCCGACGGCCAACACCGTCGCCGCCGCCGAGCATGGGATTGCTCTCCTCACCGCCATGGCTCGCAACGTCGCTCAAGCCGACGCCTCCGTCAAAGCCGGTAAATGGCTACGAAACAAGTACGTTGGAGTTTCGCTTGTTGGGAAGACCCTGGCTGTGATGGGATTCGGCAAGGTTGGATCTGAGGTGGCTCGACGAGCTAAGGGGCTTGGAATGCATGTGATAGCACACGATCCTTATGCCCCGGCGGATCGTGCTCGCGCCATCGGTGTGGAGCTTGTCAACTTCGACGAGGCTCTGGCCACTGCTGATTTCATCTCCCTGCATATGCCTCTCACTGCTGCTACTAATAAGGTTCTCAACGACGAGACGTTTGCAAAGATGAAGAAGGGAGTGAGGATTGTGAACGTTGCACGAGGTGGAGTTATTGATGAGGATGCGCTGGTCAGGGCACTTGATTCTGGAATTGTTGCTCAGGCTGCGCTTGATGTGTTCACAGTGGAGCCACCACCACAGGGCAACAAGTTGGTGTTGCATGAGAGAGTTACTGCCACCCCTCACCTTGGTGCTAGCACCATGGAAGCTCAGGAAGGAGTGGCCATTGAAATTGCTGAAGCTGTCGTGGGAGCCTTGAAAGGGGAGCTTGCTGCCACTGCTGTCAATGCACCAATGGTTCCCGCTGAGGTTTTGGCGGAACTAAAGCCTTATGTTGAACTTGCCGAGAAACTTGGGAGACTGGCTGTCCAGTTAGTGGCAGGAGGCAGTGGTGTGAAGAATGTAAAGGTTTCATATGCCTCAGCTAGAGCTCCTGATGACCTTGACACTAGACTTCTTCGAGCCATGATTACCAAGGGTCTCATTGAGCCCATATCCAGTGTTTTTGTGAATCTGGTGAATGCTGATTTCACTGCTAAACAGAGAGGACTCAGGATAACAGAAGAGCGGACTATTCTAGATGGTTCACCCGAGAATCCCCTGGACTCCATCCAGGTCCAGATTGCCAATGTGGAATCTAAATTTGCCAGTGCCATATCAGACAGTGGGGAGATAAAGGTGGAGGGTCGGGTTAAGGATGGGGTTCCCCACTTGACGAGGGTTGGATCTTTTGAAGTTGATGTGAGCTTGGAAGGTAGCATTATCCTCTGCAGACAGGTTGATCAGCCGGGTATGATAGGATTGGTTGGAAGCATTTTAGGAGAGGAGAACGTGAATGTTAGTTTCATGAGTGTCGGAAGAATTGCCCCAAGAAAGCAGGCTGTGATGGCCATTGGTGTGGATGACACACCCAGCAAGCAATCTTTGAAAAGGATTGGGGACGTTCCAGCCATTGAAGAGTTTGTGTTTCTCAAGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000166 GO:0003006 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009555 GO:0009561 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0016020 GO:0017076 GO:0022414 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

602

Amino Acids

63.23

Weight (kDa)

6.71

Isoelectric Point (pI)

35.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	62 - 371	3.5e-33	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	164 - 339	8.1e-62	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
KARI_N	PF07991	196 - 360	8.8e-07	Acetohydroxy acid isomeroreductase, NADPH-binding domain
PGDH_inter	PF19304	382 - 495	2.6e-15	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	529 - 590	3.6e-06	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19480 AT4G34200
fragaria_vesca	FvH4_2g05310
malus_domestica	MD05G1088400.v1.1
prunus_persica	Prupe.8G134300_v2.0.a1 Prupe.8G134300_v2.0.a1
pyrus_communis	pycom05g08770
rosa_chinensis	RchiOBHm_Chr6g0255451
rosa_laevigata	RLG00000014836
rosa_multiflora	Rmu_sc0002360.1_g000009
rosa_roxburghii	Rroxscaffold_7G00209970
rosa_rugosa	Rorug05G0558900
rosa_samantha	Rh6AG074800 Rh6BG069400 Rh6CG068400 Rh6DG065200
rosa_wichuraiana	Rw6G006650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	539
AccB1I	GGYRCC	2 cut(s)	323, 416
AccB7I	CCANNNNNTGG	1 cut(s)	1201
AccBSI	CCGCTC	2 cut(s)	321, 1393
AccII	CGCG	4 cut(s)	131, 161, 339, 711
AccIII	TCCGGA	1 cut(s)	315
AclI	AACGTT	1 cut(s)	858
AclWI	GGATC	5 cut(s)	417, 637, 677, 708, 1558
AcoI	YGGCCR	6 cut(s)	3, 379, 459, 750, 1047, 1713
AcsI	RAATTY	1 cut(s)	1466
AcuI	CTGAAG	2 cut(s)	248, 1083
AcyI	GRCGYC	4 cut(s)	171, 303, 417, 534
AdeI	CACNNNGTG	1 cut(s)	869
AfaI	GTAC	1 cut(s)	572
AgsI	TTSAA	7 cut(s)	370, 1055, 1084, 1163, 1559, 1753, 1781
AhdI	GACNNNNNGTC	1 cut(s)	1190
AjiI	CACGTC	1 cut(s)	9
AjnI	CCWGG	4 cut(s)	134, 600, 1425, 1437
AleI	CACNNNNGTG	1 cut(s)	1014
Alw21I	GWGCWC	3 cut(s)	279, 709, 1258
Alw26I	GTCTC	4 cut(s)	157, 812, 1177, 1310
AlwI	GGATC	5 cut(s)	417, 637, 677, 708, 1558
Ama87I	CYCGRG	2 cut(s)	269, 1415
Aor13HI	TCCGGA	1 cut(s)	315
AoxI	GGCC	6 cut(s)	3, 379, 459, 750, 1047, 1713
ApeKI	GCWGC	4 cut(s)	435, 791, 929, 1097
ApoI	RAATTY	1 cut(s)	1466
ArsI	GACNNNNNNTTYG	2 cut(s)	806, 838
Asp700I	GAANNNNTTC	1 cut(s)	233
AspLEI	GCGC	5 cut(s)	133, 419, 713, 892, 934
AspS9I	GGNCC	2 cut(s)	148, 1441
AsuC2I	CCSGG	2 cut(s)	695, 1614
AsuHPI	GGTGA	4 cut(s)	493, 1001, 1354, 1404
AsuNHI	GCTAGC	1 cut(s)	1019
AvaI	CYCGRG	2 cut(s)	269, 1415
AvaII	GGWCC	2 cut(s)	148, 1441
BaeGI	GKGCMC	1 cut(s)	904
BalI	TGGCCA	4 cut(s)	5, 752, 1049, 1715
BanI	GGYRCC	2 cut(s)	323, 416
BanII	GRGCYC	5 cut(s)	219, 269, 279, 1258, 1317
BauI	CACGAG	1 cut(s)	864
BbsI	GAAGAC	2 cut(s)	337, 602
Bbv12I	GWGCWC	3 cut(s)	279, 709, 1258
BbvCI	CCTCAGC	2 cut(s)	1131, 1246
BbvI	GCAGC	4 cut(s)	422, 778, 916, 1084
BccI	CCATC	6 cut(s)	604, 722, 1400, 1442, 1517, 1705
BceAI	ACGGC	1 cut(s)	474
BcgI	CGANNNNNNTGC	4 cut(s)	671, 705, 806, 840
BciT130I	CCWGG	4 cut(s)	136, 602, 1427, 1439
BclI	TGATCA	1 cut(s)	1606
BcnI	CCSGG	2 cut(s)	695, 1614
BcoDI	GTCTC	4 cut(s)	157, 812, 1177, 1310
BfaI	CTAG	4 cut(s)	1020, 1251, 1275, 1403
BfmI	CTRYAG	1 cut(s)	1594
BfoI	RGCGCY	1 cut(s)	420
BglI	GCCNNNNNGGC	1 cut(s)	387
BglII	AGATCT	1 cut(s)	288
Bme1390I	CCNGG	6 cut(s)	136, 602, 695, 1427, 1439, 1614
Bme18I	GGWCC	2 cut(s)	148, 1441
BmeRI	GACNNNNNGTC	1 cut(s)	1190
BmeT110I	CYCGRG	2 cut(s)	269, 1415
BmgBI	CACGTC	1 cut(s)	9
BmgT120I	GGNCC	2 cut(s)	148, 1441
BmiI	GGNNCC	6 cut(s)	325, 418, 954, 1078, 1125, 1530
BmrFI	CCNGG	6 cut(s)	136, 602, 695, 1427, 1439, 1614
BmsI	GCATC	2 cut(s)	23, 877
BmtI	GCTAGC	1 cut(s)	1023
BpiI	GAAGAC	2 cut(s)	337, 602
Bpu10I	CCTNAGC	6 cut(s)	263, 651, 924, 1035, 1131, 1246
BpuEI	CTTGAG	2 cut(s)	510, 1781
BpuMI	CCSGG	2 cut(s)	695, 1614
BsaHI	GRCGYC	4 cut(s)	171, 303, 417, 534
BsaI	GGTCTC	1 cut(s)	1310
BsaWI	WCCGGW	1 cut(s)	315
Bse118I	RCCGGY	2 cut(s)	386, 548
Bse1I	ACTGG	4 cut(s)	1191, 1195, 1323, 1473
BseAI	TCCGGA	1 cut(s)	315
BseBI	CCWGG	4 cut(s)	136, 602, 1427, 1439
BseGI	GGATG	5 cut(s)	14, 892, 1434, 1528, 1732
BseMII	CTCAG	7 cut(s)	254, 624, 938, 1049, 1122, 1260, 1390
BseNI	ACTGG	4 cut(s)	1191, 1195, 1323, 1473
BseRI	GAGGAG	6 cut(s)	49, 52, 67, 287, 488, 1664
BseSI	GKGCMC	1 cut(s)	904
BseXI	GCAGC	4 cut(s)	422, 778, 916, 1084
BseYI	CCCAGC	1 cut(s)	1736
BsgI	GTGCAG	1 cut(s)	265
Bsh1236I	CGCG	4 cut(s)	131, 161, 339, 711
BshFI	GGCC	6 cut(s)	5, 381, 461, 752, 1049, 1715
BshNI	GGYRCC	2 cut(s)	323, 416
BsiHKAI	GWGCWC	3 cut(s)	279, 709, 1258
BsiHKCI	CYCGRG	2 cut(s)	269, 1415
BsiSI	CCGG	6 cut(s)	316, 357, 387, 549, 695, 1613
BslFI	GGGAC	1 cut(s)	1778
BsmAI	GTCTC	4 cut(s)	157, 812, 1177, 1310
BsmBI	CGTCTC	1 cut(s)	812
BsmFI	GGGAC	1 cut(s)	1778
BsmI	GAATGC	2 cut(s)	669, 1351
BsnI	GGCC	6 cut(s)	5, 381, 461, 752, 1049, 1715
Bso31I	GGTCTC	1 cut(s)	1310
BsoBI	CYCGRG	2 cut(s)	269, 1415
Bsp1286I	GDGCHC	7 cut(s)	219, 269, 279, 709, 904, 1258, 1317
Bsp13I	TCCGGA	1 cut(s)	315
Bsp143I	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
Bsp19I	CCATGG	2 cut(s)	507, 1026
BspANI	GGCC	6 cut(s)	5, 381, 461, 752, 1049, 1715
BspCNI	CTCAG	7 cut(s)	255, 625, 937, 1048, 1123, 1259, 1389
BspEI	TCCGGA	1 cut(s)	315
BspFNI	CGCG	4 cut(s)	131, 161, 339, 711
BspHI	TCATGA	1 cut(s)	1671
BspLI	GGNNCC	6 cut(s)	325, 418, 954, 1078, 1125, 1530
BspMAI	CTGCAG	1 cut(s)	1598
BspOI	GCTAGC	1 cut(s)	1023
BspPI	GGATC	5 cut(s)	417, 637, 677, 708, 1558
BspQI	GCTCTTC	1 cut(s)	1383
BspT107I	GGYRCC	2 cut(s)	323, 416
BspTNI	GGTCTC	1 cut(s)	1310
BsrBI	CCGCTC	2 cut(s)	321, 1393
BsrFI	RCCGGY	2 cut(s)	386, 548
BsrI	ACTGG	4 cut(s)	1191, 1195, 1323, 1473
BssAI	RCCGGY	2 cut(s)	386, 548
BssMI	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
BssNI	GRCGYC	4 cut(s)	171, 303, 417, 534
BssSI	CACGAG	1 cut(s)	864
BssT1I	CCWWGG	5 cut(s)	327, 507, 1012, 1026, 1299
Bst2BI	CACGAG	1 cut(s)	864
Bst2UI	CCWGG	4 cut(s)	136, 602, 1427, 1439
Bst4CI	ACNGT	4 cut(s)	184, 469, 949, 1490
Bst6I	CTCTTC	2 cut(s)	1383, 1776
BstACI	GRCGYC	4 cut(s)	171, 303, 417, 534
BstC8I	GCNNGC	7 cut(s)	388, 514, 709, 1021, 1095, 1704, 1742
BstDEI	CTNAG	8 cut(s)	263, 633, 651, 924, 1035, 1131, 1246, 1376
BstDSI	CCRYGG	2 cut(s)	507, 1026
BstENI	CCTNNNNNAGG	1 cut(s)	227
BstF5I	GGATG	5 cut(s)	14, 892, 1434, 1528, 1732
BstFNI	CGCG	4 cut(s)	131, 161, 339, 711
BstH2I	RGCGCY	1 cut(s)	420
BstHHI	GCGC	5 cut(s)	133, 419, 713, 892, 934
BstKTI	GATC	7 cut(s)	291, 412, 632, 685, 703, 1553, 1609
BstMAI	GTCTC	4 cut(s)	157, 812, 1177, 1310
BstMBI	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
BstNI	CCWGG	4 cut(s)	136, 602, 1427, 1439
BstNSI	RCATGY	1 cut(s)	671
BstSCI	CCNGG	6 cut(s)	134, 600, 693, 1425, 1437, 1612
BstSFI	CTRYAG	1 cut(s)	1594
BstSLI	GKGCMC	1 cut(s)	904
BstUI	CGCG	4 cut(s)	131, 161, 339, 711
BstV1I	GCAGC	4 cut(s)	422, 778, 916, 1084
BstV2I	GAAGAC	2 cut(s)	337, 602
BstX2I	RGATCY	4 cut(s)	288, 409, 629, 1550
BstXI	CCANNNNNNTGG	2 cut(s)	721, 1723
BstYI	RGATCY	4 cut(s)	288, 409, 629, 1550
BsuRI	GGCC	6 cut(s)	5, 381, 461, 752, 1049, 1715
BtgI	CCRYGG	2 cut(s)	507, 1026
BtrI	CACGTC	1 cut(s)	9
BtsCI	GGATG	5 cut(s)	14, 892, 1434, 1528, 1732
BtsI	GCAGTG	5 cut(s)	753, 786, 1101, 1219, 1356
BtsIMutI	CAGTG	9 cut(s)	753, 786, 954, 1101, 1219, 1330, 1356, 1480, 1495
Cac8I	GCNNGC	7 cut(s)	388, 514, 709, 1021, 1095, 1704, 1742
CciI	TCATGA	1 cut(s)	1671
CfoI	GCGC	5 cut(s)	133, 419, 713, 892, 934
Cfr10I	RCCGGY	2 cut(s)	386, 548
Cfr13I	GGNCC	2 cut(s)	148, 1441
CseI	GACGC	3 cut(s)	179, 311, 542
Csp6I	GTAC	1 cut(s)	571
CspCI	CAANNNNNGTGG	2 cut(s)	412, 447
CviAII	CATG	7 cut(s)	485, 508, 668, 986, 1027, 1291, 1672
CviQI	GTAC	1 cut(s)	571
DdeI	CTNAG	8 cut(s)	263, 633, 651, 924, 1035, 1131, 1246, 1376
DinI	GGCGCC	1 cut(s)	418
DpnI	GATC	7 cut(s)	290, 411, 631, 684, 702, 1552, 1608
DpnII	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
DraIII	CACNNNGTG	1 cut(s)	869
DrdI	GACNNNNNNGTC	1 cut(s)	539
DriI	GACNNNNNGTC	1 cut(s)	1190
DseDI	GACNNNNNNGTC	1 cut(s)	539
EaeI	YGGCCR	6 cut(s)	3, 379, 459, 750, 1047, 1713
Eam1104I	CTCTTC	2 cut(s)	1383, 1776
Eam1105I	GACNNNNNGTC	1 cut(s)	1190
EarI	CTCTTC	2 cut(s)	1383, 1776
EciI	GGCGGA	3 cut(s)	145, 713, 1157
Ecl136II	GAGCTC	2 cut(s)	277, 1256
Eco130I	CCWWGG	5 cut(s)	327, 507, 1012, 1026, 1299
Eco24I	GRGCYC	5 cut(s)	219, 269, 279, 1258, 1317
Eco31I	GGTCTC	1 cut(s)	1310
Eco47I	GGWCC	2 cut(s)	148, 1441
Eco53kI	GAGCTC	2 cut(s)	277, 1256
Eco57I	CTGAAG	2 cut(s)	248, 1083
Eco88I	CYCGRG	2 cut(s)	269, 1415
EcoICRI	GAGCTC	2 cut(s)	277, 1256
EcoNI	CCTNNNNNAGG	1 cut(s)	227
EcoRII	CCWGG	4 cut(s)	134, 600, 1425, 1437
EcoT14I	CCWWGG	5 cut(s)	327, 507, 1012, 1026, 1299
EcoT22I	ATGCAT	1 cut(s)	669
EcoT38I	GRGCYC	5 cut(s)	219, 269, 279, 1258, 1317
EgeI	GGCGCC	1 cut(s)	418
EheI	GGCGCC	1 cut(s)	418
ErhI	CCWWGG	5 cut(s)	327, 507, 1012, 1026, 1299
Esp3I	CGTCTC	1 cut(s)	812
FaeI	CATG	7 cut(s)	488, 511, 671, 989, 1030, 1294, 1675
FalI	AAGNNNNNCTT	2 cut(s)	1077, 1109
FaqI	GGGAC	1 cut(s)	1778
FatI	CATG	7 cut(s)	484, 507, 667, 985, 1026, 1290, 1671
FauI	CCCGC	4 cut(s)	119, 205, 353, 1135
FauNDI	CATATG	2 cut(s)	777, 1240
FbaI	TGATCA	1 cut(s)	1606
FokI	GGATG	4 cut(s)	899, 1421, 1535, 1739
FriOI	GRGCYC	5 cut(s)	219, 269, 279, 1258, 1317
FspBI	CTAG	4 cut(s)	1020, 1251, 1275, 1403
GlaI	GCGC	5 cut(s)	132, 418, 712, 891, 933
GsaI	CCCAGC	1 cut(s)	1740
HaeII	RGCGCY	1 cut(s)	420
HaeIII	GGCC	6 cut(s)	5, 381, 461, 752, 1049, 1715
HapII	CCGG	6 cut(s)	316, 357, 387, 549, 695, 1613
HgaI	GACGC	3 cut(s)	179, 311, 542
HhaI	GCGC	5 cut(s)	133, 419, 713, 892, 934
Hin1I	GRCGYC	4 cut(s)	171, 303, 417, 534
Hin1II	CATG	7 cut(s)	488, 511, 671, 989, 1030, 1294, 1675
Hin6I	GCGC	5 cut(s)	131, 417, 711, 890, 932
HinP1I	GCGC	5 cut(s)	131, 417, 711, 890, 932
HincII	GTYRAC	1 cut(s)	733
HindII	GTYRAC	1 cut(s)	733
HinfI	GANTC	9 cut(s)	165, 350, 615, 908, 1336, 1374, 1420, 1430, 1460
HpaII	CCGG	6 cut(s)	316, 357, 387, 549, 695, 1613
HphI	GGTGA	4 cut(s)	493, 1001, 1354, 1404
Hpy166II	GTNNAC	5 cut(s)	247, 733, 856, 945, 1412
Hpy188I	TCNGA	5 cut(s)	44, 456, 634, 1486, 1682
Hpy188III	TCNNGA	9 cut(s)	226, 316, 912, 1037, 1259, 1378, 1403, 1444, 1672
Hpy8I	GTNNAC	5 cut(s)	247, 733, 856, 945, 1412
HpyAV	CCTTC	7 cut(s)	46, 106, 223, 364, 832, 1034, 1571
HpyCH4III	ACNGT	4 cut(s)	184, 469, 949, 1490
HpyCH4IV	ACGT	8 cut(s)	8, 243, 519, 573, 821, 858, 1656, 1767
HpyCH4V	TGCA	8 cut(s)	282, 667, 775, 827, 863, 985, 1115, 1596
HpyF3I	CTNAG	8 cut(s)	263, 633, 651, 924, 1035, 1131, 1246, 1376
HpySE526I	ACGT	8 cut(s)	8, 243, 519, 573, 821, 858, 1656, 1767
Hsp92I	GRCGYC	4 cut(s)	171, 303, 417, 534
Hsp92II	CATG	7 cut(s)	488, 511, 671, 989, 1030, 1294, 1675
HspAI	GCGC	5 cut(s)	131, 417, 711, 890, 932
KasI	GGCGCC	1 cut(s)	416
Kpn2I	TCCGGA	1 cut(s)	315
KroI	GCCGGC	1 cut(s)	386
KroNI	GCCGGC	1 cut(s)	388
Ksp22I	TGATCA	1 cut(s)	1606
Kzo9I	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
LguI	GCTCTTC	1 cut(s)	1383
LmnI	GCTCC	8 cut(s)	274, 318, 457, 724, 952, 1076, 1090, 1261
Lsp1109I	GCAGC	4 cut(s)	422, 778, 916, 1084
LweI	GCATC	2 cut(s)	23, 877
MaeI	CTAG	4 cut(s)	1020, 1251, 1275, 1403
MaeII	ACGT	8 cut(s)	8, 243, 519, 573, 821, 858, 1656, 1767
MaeIII	GTNAC	1 cut(s)	994
MalI	GATC	7 cut(s)	290, 411, 631, 684, 702, 1552, 1608
MbiI	CCGCTC	2 cut(s)	321, 1393
MboI	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
MboII	GAAGA	8 cut(s)	337, 607, 847, 1234, 1274, 1400, 1695, 1793
MflI	RGATCY	4 cut(s)	288, 409, 629, 1550
MhlI	GDGCHC	7 cut(s)	219, 269, 279, 709, 904, 1258, 1317
MlsI	TGGCCA	4 cut(s)	5, 752, 1049, 1715
MluCI	AATT	4 cut(s)	915, 1056, 1466, 1686
MluNI	TGGCCA	4 cut(s)	5, 752, 1049, 1715
Mly113I	GGCGCC	1 cut(s)	417
MlyI	GAGTC	4 cut(s)	159, 359, 1368, 1424
MmeI	TCCRAC	6 cut(s)	479, 556, 607, 1528, 1612, 1660
Mox20I	TGGCCA	4 cut(s)	5, 752, 1049, 1715
Mph1103I	ATGCAT	1 cut(s)	669
MroI	TCCGGA	1 cut(s)	315
MroNI	GCCGGC	1 cut(s)	386
MroXI	GAANNNNTTC	1 cut(s)	233
MscI	TGGCCA	4 cut(s)	5, 752, 1049, 1715
MseI	TTAA	2 cut(s)	447, 1518
MslI	CAYNNNNRTG	4 cut(s)	506, 719, 1014, 1721
Msp20I	TGGCCA	4 cut(s)	5, 752, 1049, 1715
MspA1I	CMGCKG	1 cut(s)	1130
MspI	CCGG	6 cut(s)	316, 357, 387, 549, 695, 1613
MspR9I	CCNGG	6 cut(s)	136, 602, 695, 1427, 1439, 1614
Mva1269I	GAATGC	2 cut(s)	669, 1351
MvaI	CCWGG	4 cut(s)	136, 602, 1427, 1439
MvnI	CGCG	4 cut(s)	131, 161, 339, 711
NaeI	GCCGGC	1 cut(s)	388
NarI	GGCGCC	1 cut(s)	417
NciI	CCSGG	2 cut(s)	695, 1614
NcoI	CCATGG	2 cut(s)	507, 1026
NdeI	CATATG	2 cut(s)	777, 1240
NdeII	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
NgoMIV	GCCGGC	1 cut(s)	386
NheI	GCTAGC	1 cut(s)	1019
NlaIII	CATG	7 cut(s)	488, 511, 671, 989, 1030, 1294, 1675
NlaIV	GGNNCC	6 cut(s)	325, 418, 954, 1078, 1125, 1530
NmeAIII	GCCGAG	5 cut(s)	183, 220, 407, 505, 1195
NsiI	ATGCAT	1 cut(s)	669
NspI	RCATGY	1 cut(s)	671
OliI	CACNNNNGTG	1 cut(s)	1014
PagI	TCATGA	1 cut(s)	1671
PciSI	GCTCTTC	1 cut(s)	1383
PcsI	WCGNNNNNNNCGW	2 cut(s)	477, 528
PctI	GAATGC	2 cut(s)	669, 1351
PdiI	GCCGGC	1 cut(s)	388
PdmI	GAANNNNTTC	1 cut(s)	233
PfeI	GAWTC	5 cut(s)	615, 908, 1336, 1420, 1460
PflMI	CCANNNNNTGG	1 cut(s)	1201
PleI	GAGTC	4 cut(s)	159, 358, 1368, 1424
PluTI	GGCGCC	1 cut(s)	420
PpsI	GAGTC	4 cut(s)	159, 358, 1368, 1424
Psp124BI	GAGCTC	2 cut(s)	279, 1258
Psp1406I	AACGTT	1 cut(s)	858
Psp6I	CCWGG	4 cut(s)	134, 600, 1425, 1437
PspFI	CCCAGC	1 cut(s)	1736
PspGI	CCWGG	4 cut(s)	134, 600, 1425, 1437
PspN4I	GGNNCC	6 cut(s)	325, 418, 954, 1078, 1125, 1530
PspPI	GGNCC	2 cut(s)	148, 1441
PstI	CTGCAG	1 cut(s)	1598
PsuI	RGATCY	4 cut(s)	288, 409, 629, 1550
RsaI	GTAC	1 cut(s)	572
RsaNI	GTAC	1 cut(s)	571
RseI	CAYNNNNRTG	4 cut(s)	506, 719, 1014, 1721
SacI	GAGCTC	2 cut(s)	279, 1258
SapI	GCTCTTC	1 cut(s)	1383
SaqAI	TTAA	2 cut(s)	447, 1518
Sau3AI	GATC	7 cut(s)	288, 409, 629, 682, 700, 1550, 1606
Sau96I	GGNCC	2 cut(s)	148, 1441
SchI	GAGTC	4 cut(s)	159, 359, 1368, 1424
ScrFI	CCNGG	6 cut(s)	136, 602, 695, 1427, 1439, 1614
SduI	GDGCHC	7 cut(s)	219, 269, 279, 709, 904, 1258, 1317
SfaNI	GCATC	2 cut(s)	23, 877
SfcI	CTRYAG	1 cut(s)	1594
SfoI	GGCGCC	1 cut(s)	418
SinI	GGWCC	2 cut(s)	148, 1441
SmiMI	CAYNNNNRTG	4 cut(s)	506, 719, 1014, 1721
SmlI	CTYRAG	2 cut(s)	525, 1796
SmoI	CTYRAG	2 cut(s)	525, 1796
Sse9I	AATT	4 cut(s)	915, 1056, 1466, 1686
SspDI	GGCGCC	1 cut(s)	416
SspMI	CTAG	4 cut(s)	1020, 1251, 1275, 1403
SstI	GAGCTC	2 cut(s)	279, 1258
StyD4I	CCNGG	6 cut(s)	134, 600, 693, 1425, 1437, 1612
StyI	CCWWGG	5 cut(s)	327, 507, 1012, 1026, 1299
TaaI	ACNGT	4 cut(s)	184, 469, 949, 1490
TaiI	ACGT	8 cut(s)	11, 246, 522, 576, 824, 861, 1659, 1770
TaqI	TCGA	6 cut(s)	168, 219, 348, 643, 738, 1285
TasI	AATT	4 cut(s)	915, 1056, 1466, 1686
TfiI	GAWTC	5 cut(s)	615, 908, 1336, 1420, 1460
Tru1I	TTAA	2 cut(s)	447, 1518
Tru9I	TTAA	2 cut(s)	447, 1518
TscAI	CASTG	9 cut(s)	760, 793, 954, 1108, 1219, 1330, 1363, 1480, 1495
TseI	GCWGC	4 cut(s)	435, 791, 929, 1097
TspDTI	ATGAA	4 cut(s)	754, 848, 1227, 1660
TspGWI	ACGGA	1 cut(s)	529
TspRI	CASTG	9 cut(s)	760, 793, 954, 1108, 1219, 1330, 1363, 1480, 1495
Van91I	CCANNNNNTGG	1 cut(s)	1201
VpaK11BI	GGWCC	2 cut(s)	148, 1441
XagI	CCTNNNNNAGG	1 cut(s)	227
XapI	RAATTY	1 cut(s)	1466
XbaI	TCTAGA	1 cut(s)	1402
XceI	RCATGY	1 cut(s)	671
XcmI	CCANNNNNNNNNTGG	1 cut(s)	142
XmnI	GAANNNNTTC	1 cut(s)	233
XspI	CTAG	4 cut(s)	1020, 1251, 1275, 1403
Zsp2I	ATGCAT	1 cut(s)	669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.