Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0013945

Physical Location & Seq

Genomic Coordinates Reverse (-)

18862219 .. 18865210

Gene Structure 2992 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1088400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGACCACATCAGCGTTCCAAATCCGCTCCCCATTTTCCCGAAACCCCGCCCTCTCTTTCTCCTCCAAGCCCTCCCTCTCCGCTTTCTCAGTAACTCCCGCCTCGCGCCGCCGGGCTCCTCCGCGGCTGGTCGTGGTGCTGTCTGCTGCGAACCTCGATGCCAAGCCCACCGTCCTCGTCTCAGAGAAGCTCGGAGAACCCGGAATCAACCTCCTCAAGCAGTTCGCTAATGTCGACTGCTCCTACAATCTCAGCCCCGAGGAGCTCTGCACCAAGATCTCGCTCTGCGACGCGTTGATCGTGCGGAGCGGGACCAAGGTCACCCGCGAGGTGTTCGAGTCCTCCGGCGGGAGGTTGAAGGTTGTGGGAAGAGCTGGCGTCGGCATCGACAACGTGGATCTGGCTGCGGCGACTGAGTTCGGGTGCTTGGTGGTGAACGCCCCCACGGCCAACACCGTTGCGGCCGCCGAGCACGGGATTGCTCTCTTGACAGCCATGGCTCGCAACGTCGCTCAGGCCGACGCGTCTGTGAAAGCCGGGAAATGGGAGAGGAACAAGTATGTAGGTGTTTCCCTTGTTGGGAAGACCTTAGCCGTGATGGGGTTCGGGAAAGTTGGATCTGAAGTGGCTCGGCGTGCCAAGGGGCTCGGTATGCACGTGATTTCCCACGACCCGTACGCCCCGGCGGATCGTGCTCGTGCCATTGGTGTGGAGCTTGTGAGCTTCGATGAGGCTTTGGCCACCGCCGATTTCATATCTTTGCACATGCCTCTCACTCCAGCCACCAACAAGGTTCTAAATGATGACACTTTTGCTAAGATGAAGAAGGGAGTTCGAATCGTGAACGTCGCTCGTGGTGGTGTCATTGATGAGGATGCACTCCTCAGGGCGCTCGATGCCGGAATTGTTGCTCAGGCTGCTCTTGATGTGTTCACGGTGGAGCCACCGCCACAGGGCAACAAGTTGGTGTTGCATGAGAGAGTTACTGCTACTCCTCACCTTGGTGCCAGTACCATGGAAGCACAGGAAGGTGTGGCCGTTGAAATTGCTGAAGCTGTCGTCGGAGCCTTGAAAGGGGAGCTTGCCGCCACTGCAGTCAATGCACCAATGGTTCCCGCTGAGGTTTTGACAGAACTGAAGCCTTATGTTGTGCTTGCTGAGAAGCTTGGAAGACTGGCTGTCCAGTTAGTGGCAGGAGGTAGCGGTGTGAAAAATGTCAAGGTCTCATATGCGTCAGCTAGGGCTCCTGATGACCTTGACACTAGGCTGCTCCGAGCCATGATTACCAAGGGTCTCATTGAGCCCATATCCAGCGTTTTCGTGAATCTGGTTAATGCAGATTTCACTGCTAAACAGAGAGGACTCAGGATAACAGAAGAGCGAACAATGCTTGATGGTTCGCCCGAAAGTCCACTTGATTCCATCCAAGTGCAGATTGCCAACGTGGAATCCAAATTTGCCAGTGCGATATCAGAGAGCGGAGAGATCACGGTGGAGGGTCGGGTTAAGGATGGGGTCCCTCACTTGACAAAGGTTGGATCATTCGAAGTTGATGTGAGTTTGGAAGGTAGCATAATCCTCTGCAGACAGGTTGATCAGCCGGGTATGATAGGACTGGTCGGAAGCATTTTAGGAGAGGAGAATGTGAATGTCAGTTTCATGAGCGTTGGAAGGATTGCCCCGCGAAAGCAGGCTGTGATGGCAATTGGAGTGGATGAACAACCCAGCAAGCAGTCTTTGAAAAGGATTGGGGATGTTCCAGCCATTGAAGAGTTTGTTTTCCTCAAGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000166 GO:0003006 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009555 GO:0009561 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0016020 GO:0017076 GO:0022414 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

598

Amino Acids

62.93

Weight (kDa)

6.7

Isoelectric Point (pI)

37.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	58 - 367	3.9e-34	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	160 - 335	7.5e-62	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
KARI_N	PF07991	192 - 356	2.5e-06	Acetohydroxy acid isomeroreductase, NADPH-binding domain
PGDH_inter	PF19304	378 - 489	8.3e-15	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	525 - 587	1.3e-06	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0013945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19480 AT4G34200
fragaria_vesca	FvH4_2g05310
malus_domestica	MD05G1088400.v1.1
prunus_persica	Prupe.8G134300_v2.0.a1 Prupe.8G134300_v2.0.a1
pyrus_communis	pycom05g08770
rosa_chinensis	RchiOBHm_Chr6g0255451
rosa_laevigata	RLG00000014836
rosa_multiflora	Rmu_sc0002360.1_g000009
rosa_roxburghii	Rroxscaffold_7G00209970
rosa_rugosa	Rorug05G0558900
rosa_samantha	Rh6AG074800 Rh6BG069400 Rh6CG068400 Rh6DG065200
rosa_wichuraiana	Rw6G006650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1004
AccB7I	CCANNNNNTGG	1 cut(s)	1189
AccBSI	CCGCTC	3 cut(s)	27, 309, 1479
AccI	GTMKAC	1 cut(s)	234
AccII	CGCG	6 cut(s)	106, 124, 293, 327, 524, 1684
AclWI	GGATC	4 cut(s)	405, 625, 696, 1546
AcoI	YGGCCR	4 cut(s)	447, 462, 738, 1035
AcsI	RAATTY	1 cut(s)	1454
AcuI	CTGAAG	3 cut(s)	642, 1071, 1157
AcvI	CACGTG	1 cut(s)	658
AcyI	GRCGYC	1 cut(s)	378
AfaI	GTAC	2 cut(s)	677, 1012
AflIII	ACRYGT	2 cut(s)	291, 522
AgsI	TTSAA	5 cut(s)	358, 1043, 1072, 1741, 1769
AhdI	GACNNNNNGTC	1 cut(s)	1178
AleI	CACNNNNGTG	1 cut(s)	1002
AluBI	AGCT	9 cut(s)	190, 265, 374, 715, 723, 1055, 1081, 1165, 1238
AluI	AGCT	9 cut(s)	190, 265, 374, 715, 723, 1055, 1081, 1165, 1238
Alw21I	GWGCWC	3 cut(s)	267, 474, 697
Alw26I	GTCTC	3 cut(s)	184, 1228, 1298
AlwI	GGATC	4 cut(s)	405, 625, 696, 1546
Ama87I	CYCGRG	1 cut(s)	257
AoxI	GGCC	5 cut(s)	447, 462, 516, 738, 1035
ApeKI	GCWGC	4 cut(s)	146, 404, 917, 1267
ApoI	RAATTY	1 cut(s)	1454
AspLEI	GCGC	2 cut(s)	108, 892
AspS9I	GGNCC	2 cut(s)	312, 1516
AsuC2I	CCSGG	5 cut(s)	113, 201, 538, 683, 1602
AsuHPI	GGTGA	3 cut(s)	313, 445, 989
AsuII	TTCGAA	2 cut(s)	835, 1545
AvaI	CYCGRG	1 cut(s)	257
AvaII	GGWCC	2 cut(s)	312, 1516
AxyI	CCTNAGG	1 cut(s)	884
BalI	TGGCCA	1 cut(s)	740
BanI	GGYRCC	1 cut(s)	1004
BanII	GRGCYC	5 cut(s)	118, 267, 648, 1246, 1305
BauI	CACGAG	2 cut(s)	696, 852
BbrPI	CACGTG	1 cut(s)	658
BbsI	GAAGAC	2 cut(s)	590, 1177
Bbv12I	GWGCWC	3 cut(s)	267, 474, 697
BbvCI	CCTCAGC	1 cut(s)	1119
BbvI	GCAGC	4 cut(s)	133, 391, 904, 1254
BccI	CCATC	5 cut(s)	592, 1388, 1430, 1505, 1693
BceAI	ACGGC	3 cut(s)	462, 578, 1022
BclI	TGATCA	1 cut(s)	1594
BcnI	CCSGG	5 cut(s)	113, 201, 538, 683, 1602
BcoDI	GTCTC	3 cut(s)	184, 1228, 1298
BfaI	CTAG	2 cut(s)	1239, 1263
BfmI	CTRYAG	2 cut(s)	1092, 1582
BfoI	RGCGCY	1 cut(s)	893
BglI	GCCNNNNNGGC	1 cut(s)	446
BglII	AGATCT	1 cut(s)	276
Bme1390I	CCNGG	5 cut(s)	113, 201, 538, 683, 1602
Bme18I	GGWCC	2 cut(s)	312, 1516
BmeRI	GACNNNNNGTC	1 cut(s)	1178
BmeT110I	CYCGRG	1 cut(s)	257
BmgT120I	GGNCC	2 cut(s)	312, 1516
BmiI	GGNNCC	9 cut(s)	117, 313, 942, 1006, 1066, 1113, 1245, 1517, 1518
BmrFI	CCNGG	5 cut(s)	113, 201, 538, 683, 1602
BmsI	GCATC	4 cut(s)	148, 393, 865, 886
BoxI	GACNNNNGTC	1 cut(s)	317
BpiI	GAAGAC	2 cut(s)	590, 1177
BpmI	CTGGAG	1 cut(s)	762
Bpu10I	CCTNAGC	4 cut(s)	513, 589, 912, 1119
Bpu14I	TTCGAA	2 cut(s)	835, 1545
BpuEI	CTTGAG	2 cut(s)	200, 1769
BpuMI	CCSGG	5 cut(s)	113, 201, 538, 683, 1602
BsaAI	YACGTR	1 cut(s)	658
BsaHI	GRCGYC	1 cut(s)	378
BsaI	GGTCTC	2 cut(s)	1228, 1298
BsaXI	ACNNNNNCTCC	2 cut(s)	976, 1006
Bse1I	ACTGG	5 cut(s)	1008, 1179, 1183, 1461, 1620
Bse21I	CCTNAGG	1 cut(s)	884
BseGI	GGATG	5 cut(s)	880, 1422, 1516, 1720, 1759
BseNI	ACTGG	5 cut(s)	1008, 1179, 1183, 1461, 1620
BseRI	GAGGAG	7 cut(s)	52, 108, 203, 275, 872, 984, 1652
BseX3I	CGGCCG	1 cut(s)	462
BseXI	GCAGC	4 cut(s)	133, 391, 904, 1254
BseYI	CCCAGC	1 cut(s)	1724
BsgI	GTGCAG	2 cut(s)	253, 1451
Bsh1236I	CGCG	6 cut(s)	106, 124, 293, 327, 524, 1684
Bsh1285I	CGRYCG	1 cut(s)	465
BshFI	GGCC	5 cut(s)	449, 464, 518, 740, 1037
BshNI	GGYRCC	1 cut(s)	1004
BsiEI	CGRYCG	1 cut(s)	465
BsiHKAI	GWGCWC	3 cut(s)	267, 474, 697
BsiHKCI	CYCGRG	1 cut(s)	257
BsiSI	CCGG	7 cut(s)	112, 201, 345, 537, 683, 900, 1601
BsiWI	CGTACG	1 cut(s)	675
BslFI	GGGAC	2 cut(s)	325, 1502
BsmAI	GTCTC	3 cut(s)	184, 1228, 1298
BsmBI	CGTCTC	1 cut(s)	184
BsmFI	GGGAC	2 cut(s)	325, 1502
BsnI	GGCC	5 cut(s)	449, 464, 518, 740, 1037
Bso31I	GGTCTC	2 cut(s)	1228, 1298
BsoBI	CYCGRG	1 cut(s)	257
Bsp119I	TTCGAA	2 cut(s)	835, 1545
Bsp1286I	GDGCHC	7 cut(s)	118, 267, 474, 648, 697, 1246, 1305
Bsp143I	GATC	8 cut(s)	276, 297, 397, 617, 688, 1485, 1538, 1594
Bsp19I	CCATGG	2 cut(s)	495, 1014
BspANI	GGCC	5 cut(s)	449, 464, 518, 740, 1037
BspFNI	CGCG	6 cut(s)	106, 124, 293, 327, 524, 1684
BspHI	TCATGA	1 cut(s)	1659
BspLI	GGNNCC	9 cut(s)	117, 313, 942, 1006, 1066, 1113, 1245, 1517, 1518
BspMAI	CTGCAG	2 cut(s)	1096, 1586
BspPI	GGATC	4 cut(s)	405, 625, 696, 1546
BspQI	GCTCTTC	2 cut(s)	364, 1371
BspT104I	TTCGAA	2 cut(s)	835, 1545
BspT107I	GGYRCC	1 cut(s)	1004
BspTNI	GGTCTC	2 cut(s)	1228, 1298
BsrBI	CCGCTC	3 cut(s)	27, 309, 1479
BsrI	ACTGG	5 cut(s)	1008, 1179, 1183, 1461, 1620
BssMI	GATC	8 cut(s)	276, 297, 397, 617, 688, 1485, 1538, 1594
BssNI	GRCGYC	1 cut(s)	378
BssSI	CACGAG	2 cut(s)	696, 852
BssT1I	CCWWGG	6 cut(s)	315, 495, 639, 1000, 1014, 1287
Bst2BI	CACGAG	2 cut(s)	696, 852
Bst4CI	ACNGT	4 cut(s)	172, 457, 937, 1492
Bst6I	CTCTTC	3 cut(s)	364, 1371, 1764
BstACI	GRCGYC	1 cut(s)	378
BstAPI	GCANNNNNTGC	1 cut(s)	1100
BstBAI	YACGTR	1 cut(s)	658
BstBI	TTCGAA	2 cut(s)	835, 1545
BstC8I	GCNNGC	7 cut(s)	376, 502, 636, 1083, 1155, 1692, 1730
BstDSI	CCRYGG	4 cut(s)	122, 444, 495, 1014
BstEII	GGTNACC	1 cut(s)	319
BstF5I	GGATG	5 cut(s)	880, 1422, 1516, 1720, 1759
BstFNI	CGCG	6 cut(s)	106, 124, 293, 327, 524, 1684
BstH2I	RGCGCY	1 cut(s)	893
BstHHI	GCGC	2 cut(s)	108, 892
BstKTI	GATC	8 cut(s)	279, 300, 400, 620, 691, 1488, 1541, 1597
BstMAI	GTCTC	3 cut(s)	184, 1228, 1298
BstMBI	GATC	8 cut(s)	276, 297, 397, 617, 688, 1485, 1538, 1594
BstMCI	CGRYCG	1 cut(s)	465
BstNSI	RCATGY	1 cut(s)	769
BstPAI	GACNNNNGTC	1 cut(s)	317
BstPI	GGTNACC	1 cut(s)	319
BstSCI	CCNGG	5 cut(s)	111, 199, 536, 681, 1600
BstSFI	CTRYAG	2 cut(s)	1092, 1582
BstUI	CGCG	6 cut(s)	106, 124, 293, 327, 524, 1684
BstV1I	GCAGC	4 cut(s)	133, 391, 904, 1254
BstV2I	GAAGAC	2 cut(s)	590, 1177
BstX2I	RGATCY	3 cut(s)	276, 397, 617
BstXI	CCANNNNNNTGG	2 cut(s)	709, 1015
BstYI	RGATCY	3 cut(s)	276, 397, 617
BstZI	CGGCCG	1 cut(s)	462
Bsu36I	CCTNAGG	1 cut(s)	884
BsuRI	GGCC	5 cut(s)	449, 464, 518, 740, 1037
BtgI	CCRYGG	4 cut(s)	122, 444, 495, 1014
BtsCI	GGATG	5 cut(s)	880, 1422, 1516, 1720, 1759
BtsI	GCAGTG	2 cut(s)	1089, 1344
BtsIMutI	CAGTG	3 cut(s)	1089, 1344, 1468
Cac8I	GCNNGC	7 cut(s)	376, 502, 636, 1083, 1155, 1692, 1730
CciI	TCATGA	1 cut(s)	1659
CciNI	GCGGCCGC	1 cut(s)	462
CfoI	GCGC	2 cut(s)	108, 892
Cfr13I	GGNCC	2 cut(s)	312, 1516
Cfr42I	CCGCGG	1 cut(s)	125
CseI	GACGC	5 cut(s)	299, 367, 513, 530, 1221
Csp6I	GTAC	2 cut(s)	676, 1011
CspCI	CAANNNNNGTGG	2 cut(s)	1692, 1727
CviAII	CATG	6 cut(s)	496, 766, 974, 1015, 1279, 1660
CviQI	GTAC	2 cut(s)	676, 1011
DpnI	GATC	8 cut(s)	278, 299, 399, 619, 690, 1487, 1540, 1596
DpnII	GATC	8 cut(s)	276, 297, 397, 617, 688, 1485, 1538, 1594
DriI	GACNNNNNGTC	1 cut(s)	1178
EaeI	YGGCCR	4 cut(s)	447, 462, 738, 1035
EagI	CGGCCG	1 cut(s)	462
Eam1104I	CTCTTC	3 cut(s)	364, 1371, 1764
Eam1105I	GACNNNNNGTC	1 cut(s)	1178
EarI	CTCTTC	3 cut(s)	364, 1371, 1764
EciI	GGCGGA	1 cut(s)	701
Ecl136II	GAGCTC	1 cut(s)	265
EclXI	CGGCCG	1 cut(s)	462
Eco130I	CCWWGG	6 cut(s)	315, 495, 639, 1000, 1014, 1287
Eco24I	GRGCYC	5 cut(s)	118, 267, 648, 1246, 1305
Eco31I	GGTCTC	2 cut(s)	1228, 1298
Eco32I	GATATC	1 cut(s)	1470
Eco47I	GGWCC	2 cut(s)	312, 1516
Eco52I	CGGCCG	1 cut(s)	462
Eco53kI	GAGCTC	1 cut(s)	265
Eco57I	CTGAAG	3 cut(s)	642, 1071, 1157
Eco72I	CACGTG	1 cut(s)	658
Eco81I	CCTNAGG	1 cut(s)	884
Eco88I	CYCGRG	1 cut(s)	257
Eco91I	GGTNACC	1 cut(s)	319
EcoICRI	GAGCTC	1 cut(s)	265
EcoO109I	RGGNCCY	1 cut(s)	1516
EcoO65I	GGTNACC	1 cut(s)	319
EcoRV	GATATC	1 cut(s)	1470
EcoT14I	CCWWGG	6 cut(s)	315, 495, 639, 1000, 1014, 1287
EcoT38I	GRGCYC	5 cut(s)	118, 267, 648, 1246, 1305
ErhI	CCWWGG	6 cut(s)	315, 495, 639, 1000, 1014, 1287
Esp3I	CGTCTC	1 cut(s)	184
FaeI	CATG	6 cut(s)	499, 769, 977, 1018, 1282, 1663
FalI	AAGNNNNNCTT	2 cut(s)	1065, 1097
FaqI	GGGAC	2 cut(s)	325, 1502
FatI	CATG	6 cut(s)	495, 765, 973, 1014, 1278, 1659
FauI	CCCGC	7 cut(s)	55, 106, 302, 332, 341, 1123, 1689
FauNDI	CATATG	1 cut(s)	1228
FbaI	TGATCA	1 cut(s)	1594
FblI	GTMKAC	1 cut(s)	234
FokI	GGATG	5 cut(s)	887, 1409, 1523, 1727, 1766
FriOI	GRGCYC	5 cut(s)	118, 267, 648, 1246, 1305
FspBI	CTAG	2 cut(s)	1239, 1263
GlaI	GCGC	2 cut(s)	107, 891
GsaI	CCCAGC	1 cut(s)	1728
GsuI	CTGGAG	1 cut(s)	762
HaeII	RGCGCY	1 cut(s)	893
HaeIII	GGCC	5 cut(s)	449, 464, 518, 740, 1037
HapII	CCGG	7 cut(s)	112, 201, 345, 537, 683, 900, 1601
HgaI	GACGC	5 cut(s)	299, 367, 513, 530, 1221
HhaI	GCGC	2 cut(s)	108, 892
Hin1I	GRCGYC	1 cut(s)	378
Hin1II	CATG	6 cut(s)	499, 769, 977, 1018, 1282, 1663
Hin6I	GCGC	2 cut(s)	106, 890
HinP1I	GCGC	2 cut(s)	106, 890
HincII	GTYRAC	1 cut(s)	235
HindII	GTYRAC	1 cut(s)	235
HindIII	AAGCTT	1 cut(s)	1163
HinfI	GANTC	7 cut(s)	204, 338, 837, 1324, 1362, 1418, 1448
HpaII	CCGG	7 cut(s)	112, 201, 345, 537, 683, 900, 1601
HphI	GGTGA	3 cut(s)	313, 445, 989
Hpy166II	GTNNAC	5 cut(s)	235, 436, 844, 933, 1412
Hpy188I	TCNGA	7 cut(s)	184, 194, 622, 1064, 1274, 1474, 1622
Hpy188III	TCNNGA	9 cut(s)	39, 487, 607, 841, 923, 1247, 1321, 1366, 1660
Hpy8I	GTNNAC	5 cut(s)	235, 436, 844, 933, 1412
Hpy99I	CGWCG	6 cut(s)	293, 383, 512, 524, 851, 1064
HpyAV	CCTTC	5 cut(s)	352, 820, 1022, 1559, 1665
HpyCH4III	ACNGT	4 cut(s)	172, 457, 937, 1492
HpyCH4IV	ACGT	5 cut(s)	393, 507, 657, 846, 1443
HpySE526I	ACGT	5 cut(s)	393, 507, 657, 846, 1443
Hsp92I	GRCGYC	1 cut(s)	378
Hsp92II	CATG	6 cut(s)	499, 769, 977, 1018, 1282, 1663
HspAI	GCGC	2 cut(s)	106, 890
KflI	GGGWCCC	1 cut(s)	1516
Ksp22I	TGATCA	1 cut(s)	1594
KspI	CCGCGG	1 cut(s)	125
Kzo9I	GATC	8 cut(s)	276, 297, 397, 617, 688, 1485, 1538, 1594
LguI	GCTCTTC	2 cut(s)	364, 1371
Lsp1109I	GCAGC	4 cut(s)	133, 391, 904, 1254
LweI	GCATC	4 cut(s)	148, 393, 865, 886
MaeI	CTAG	2 cut(s)	1239, 1263
MaeII	ACGT	5 cut(s)	393, 507, 657, 846, 1443
MaeIII	GTNAC	3 cut(s)	91, 319, 982
MalI	GATC	8 cut(s)	278, 299, 399, 619, 690, 1487, 1540, 1596
MbiI	CCGCTC	3 cut(s)	27, 309, 1479
MboI	GATC	8 cut(s)	276, 297, 397, 617, 688, 1485, 1538, 1594
MboII	GAAGA	6 cut(s)	381, 595, 835, 1182, 1388, 1781
MfeI	CAATTG	1 cut(s)	1704
MflI	RGATCY	3 cut(s)	276, 397, 617
MhlI	GDGCHC	7 cut(s)	118, 267, 474, 648, 697, 1246, 1305
MlsI	TGGCCA	1 cut(s)	740
MluCI	AATT	4 cut(s)	903, 1044, 1454, 1704
MluI	ACGCGT	2 cut(s)	291, 522
MluNI	TGGCCA	1 cut(s)	740
MlyI	GAGTC	2 cut(s)	347, 1356
MmeI	TCCRAC	5 cut(s)	595, 1042, 1516, 1600, 1648
Mox20I	TGGCCA	1 cut(s)	740
MscI	TGGCCA	1 cut(s)	740
MseI	TTAA	2 cut(s)	1332, 1506
MslI	CAYNNNNRTG	3 cut(s)	707, 1002, 1427
Msp20I	TGGCCA	1 cut(s)	740
MspA1I	CMGCKG	2 cut(s)	124, 1118
MspI	CCGG	7 cut(s)	112, 201, 345, 537, 683, 900, 1601
MspR9I	CCNGG	5 cut(s)	113, 201, 538, 683, 1602
MunI	CAATTG	1 cut(s)	1704
MvnI	CGCG	6 cut(s)	106, 124, 293, 327, 524, 1684
NciI	CCSGG	5 cut(s)	113, 201, 538, 683, 1602
NcoI	CCATGG	2 cut(s)	495, 1014
NdeI	CATATG	1 cut(s)	1228
NdeII	GATC	8 cut(s)	276, 297, 397, 617, 688, 1485, 1538, 1594
NlaIII	CATG	6 cut(s)	499, 769, 977, 1018, 1282, 1663
NlaIV	GGNNCC	9 cut(s)	117, 313, 942, 1006, 1066, 1113, 1245, 1517, 1518
NmeAIII	GCCGAG	2 cut(s)	493, 610
NmuCI	GTSAC	1 cut(s)	319
NotI	GCGGCCGC	1 cut(s)	462
NspI	RCATGY	1 cut(s)	769
NspV	TTCGAA	2 cut(s)	835, 1545
OliI	CACNNNNGTG	1 cut(s)	1002
PagI	TCATGA	1 cut(s)	1659
PciSI	GCTCTTC	2 cut(s)	364, 1371
PcsI	WCGNNNNNNNCGW	4 cut(s)	231, 297, 516, 654
PfeI	GAWTC	5 cut(s)	204, 837, 1324, 1418, 1448
Pfl23II	CGTACG	1 cut(s)	675
PflMI	CCANNNNNTGG	1 cut(s)	1189
PleI	GAGTC	2 cut(s)	346, 1356
PmaCI	CACGTG	1 cut(s)	658
PmlI	CACGTG	1 cut(s)	658
PpsI	GAGTC	2 cut(s)	346, 1356
Ppu21I	YACGTR	1 cut(s)	658
PpuMI	RGGWCCY	1 cut(s)	1516
PshAI	GACNNNNGTC	1 cut(s)	317
Psp124BI	GAGCTC	1 cut(s)	267
Psp5II	RGGWCCY	1 cut(s)	1516
PspCI	CACGTG	1 cut(s)	658
PspEI	GGTNACC	1 cut(s)	319
PspFI	CCCAGC	1 cut(s)	1724
PspLI	CGTACG	1 cut(s)	675
PspN4I	GGNNCC	9 cut(s)	117, 313, 942, 1006, 1066, 1113, 1245, 1517, 1518
PspPI	GGNCC	2 cut(s)	312, 1516
PspPPI	RGGWCCY	1 cut(s)	1516
PstI	CTGCAG	2 cut(s)	1096, 1586
PsuI	RGATCY	3 cut(s)	276, 397, 617
RsaI	GTAC	2 cut(s)	677, 1012
RsaNI	GTAC	2 cut(s)	676, 1011
RseI	CAYNNNNRTG	3 cut(s)	707, 1002, 1427
SacI	GAGCTC	1 cut(s)	267
SacII	CCGCGG	1 cut(s)	125
SalI	GTCGAC	1 cut(s)	233
SapI	GCTCTTC	2 cut(s)	364, 1371
SaqAI	TTAA	2 cut(s)	1332, 1506
Sau3AI	GATC	8 cut(s)	276, 297, 397, 617, 688, 1485, 1538, 1594
Sau96I	GGNCC	2 cut(s)	312, 1516
SchI	GAGTC	2 cut(s)	347, 1356
ScrFI	CCNGG	5 cut(s)	113, 201, 538, 683, 1602
SduI	GDGCHC	7 cut(s)	118, 267, 474, 648, 697, 1246, 1305
SfaNI	GCATC	4 cut(s)	148, 393, 865, 886
SfcI	CTRYAG	2 cut(s)	1092, 1582
Sfr303I	CCGCGG	1 cut(s)	125
SfuI	TTCGAA	2 cut(s)	835, 1545
SgrBI	CCGCGG	1 cut(s)	125
SinI	GGWCC	2 cut(s)	312, 1516
SmiMI	CAYNNNNRTG	3 cut(s)	707, 1002, 1427
SmlI	CTYRAG	2 cut(s)	215, 1784
SmoI	CTYRAG	2 cut(s)	215, 1784
Sse9I	AATT	4 cut(s)	903, 1044, 1454, 1704
SspMI	CTAG	2 cut(s)	1239, 1263
SstI	GAGCTC	1 cut(s)	267
StyD4I	CCNGG	5 cut(s)	111, 199, 536, 681, 1600
StyI	CCWWGG	6 cut(s)	315, 495, 639, 1000, 1014, 1287
TaaI	ACNGT	4 cut(s)	172, 457, 937, 1492
TaiI	ACGT	5 cut(s)	396, 510, 660, 849, 1446
TaqI	TCGA	8 cut(s)	156, 234, 336, 387, 726, 835, 894, 1545
TasI	AATT	4 cut(s)	903, 1044, 1454, 1704
TauI	GCSGC	6 cut(s)	111, 127, 410, 464, 467, 1088
TfiI	GAWTC	5 cut(s)	204, 837, 1324, 1418, 1448
Tru1I	TTAA	2 cut(s)	1332, 1506
Tru9I	TTAA	2 cut(s)	1332, 1506
TscAI	CASTG	3 cut(s)	1096, 1351, 1468
TseFI	GTSAC	1 cut(s)	319
TseI	GCWGC	4 cut(s)	146, 404, 917, 1267
Tsp45I	GTSAC	1 cut(s)	319
TspDTI	ATGAA	4 cut(s)	742, 836, 1648, 1731
TspRI	CASTG	3 cut(s)	1096, 1351, 1468
Van91I	CCANNNNNTGG	1 cut(s)	1189
VpaK11BI	GGWCC	2 cut(s)	312, 1516
XapI	RAATTY	1 cut(s)	1454
XceI	RCATGY	1 cut(s)	769
XmiI	GTMKAC	1 cut(s)	234
XspI	CTAG	2 cut(s)	1239, 1263

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.