Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0013945

Physical Location & Seq

Genomic Coordinates Forward (+)

6749299 .. 6752147

Gene Structure 2849 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG068400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1797 bp

ATGGCCACGTCAGCATCCCAAACCCTCCGCTCCCCATTTCTCCGCAACCCTACTTCCTCCCTTTCTCTCTCCTCCAAGAACCCCCTCGCCTTCTCCCTCCCCTCGCGCCGCCGCCTCGCCGCGCCGAGACTTGTCGTGGTGTTCTCGGCGGCGAGGCTCGACGCCAAGCCGACGGTTTTGGTCGCCGAGAAGCTCGGCGAGGCCGGGCTGGAACTGTTAAAGGAGTTTGCGAATGTGGACTGCTCGTATAACCTGAGCCCCGAGGAGCTCTGCACGAAGATCTCGCTCTGCGACGCGTTGATTGTCCGGAGCGGGACGAAGGTCAGCCGCGAGGTGTTTGAGTCCTCCGGCGGGAGGTTGAAGGTGGTCGGGAGAGCCGGCGTGGGGATTGATAATGTGGATCTGGGCGCCGCGACTGAGTTTGGTTGTTTGGTGGTTAATGCTCCGACGGCCAATACCGTCGCCGCGGCAGAGCATGGGATTGCTCTCCTCACCGCCATGGCTCGCAACGTGGCTCAGGCTGATGCTTCTGTTAAAGCTGGTAAGTGGCAGCGAAACAAGTATGTGGGAGTTTCGCTGGTTGGGAAAACCCTGGCTGTCATGGGATTTGGAAAGGTTGGATCTGAAGTGGCTCGGCGTGCTAAGGGGCTTGGTATGCATGTGATTGCACACGACCCTTATGCCCCGGCTGATCGTGCTCGTGCAATTGGTGTGGAGCTTGTGAGCTTCGATGAGGCTTTGGCCACTGCGGATTTCATCTCCCTGCATATGCCTCTCACTGCTGCTACAAATAAGGTTCTCAACGACGAGACGTTTGCGAAGATGAAGAAAGGAGTGAGAATTGTGAATGTTGCGCGTGGTGGTGTTATTGACGAAGATGCACTGGTCAAGGCACTTGATTCGGGAATTGTTGCTCAGGCTGCTCTTGATGTGTTCACTGTCGAGCCACCGCCACAGGGCAACAAGTTGGTGTTGCATGAGAGAGTTACTGCCACCCCTCACCTTGGTGCTAGTACCATGGAAGCACAGGAAGGAGTGGCCATTGAAATTGCTGAAGCCGTTGTTGGAGCCTTAAAAGGCGAGCTTGCTGCCACTGCAGTCAATGCACCAATGGTTCCCGCTGAGGTTTTGGCGGAACTGAAGCCTTATGTTGAACTTGCTGAGAAACTTGGGAGACTGGCTGTCCAGTTAGTGGCAGGAGGCAGTGGTGTGAAGAATGTAAAGGTTTCATATGCTTCAGCTAGAGCTCCTGATGACCTTGACACTAGACTTCTTCGAGCCATGATTACCAAGGGTCTCATTGAGCCAATATCCAGTGTTTTTGTGAATCTGGTCAATGCTGATTTCACTGCTAAGCAGAGAGGACTCAGGATAACAGAAGAGCGGACCATTCTAGATGGTTCACCCGAGAGTCCCCTAGACTCCATCCAAGTTCAGATTGCTAATGTGGAATCCAAATTTGCCAGTGCCATATCAGACAGTGGGGAGATAAAGGTGGAGGGTCGGGTTAAGGATGGGGTTCCCCACTTGACGAGGGTTGGATCTTTTGAAGTTGATGTGAGCTTGGAAGGTAGTATTATACTCTGCAGACAGGTTGATCAGCCAGGTATGATAGGATTGGTTGGAAGCATTTTAGGAGAGGAGAATGTGAACGTTAGTTTCATGAGTGTCGGAAGAATTGCCCCAAGAAAGCAGGCTGTGATGGCAATTGGTGTGGATGACCAACCCAGCAAGCAATCTTTGAAAAGGATTGGGGATGTTCCAGCCATTGAAGAGTTTGTTTTCCTCAAGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000166 GO:0003006 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009553 GO:0009555 GO:0009561 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0016020 GO:0017076 GO:0022414 GO:0030554 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00260 ko00680 ko01100 ko01120 ko01130 ko01200 ko01230 map00260 map00680 map01100 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

2-Hacid_dh 2-Hacid_dh_C ACT

Protein Analysis

598

Amino Acids

63.0

Weight (kDa)

6.71

Isoelectric Point (pI)

37.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
2-Hacid_dh	PF00389	59 - 368	2e-33	D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C	PF02826	161 - 336	6.8e-63	D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
KARI_N	PF07991	193 - 357	7.4e-07	Acetohydroxy acid isomeroreductase, NADPH-binding domain
PGDH_inter	PF19304	379 - 492	3.1e-15	D-3-phosphoglycerate dehydrogenase intervening domain
ACT	PF01842	526 - 587	2.5e-06	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0013945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19480 AT4G34200
fragaria_vesca	FvH4_2g05310
malus_domestica	MD05G1088400.v1.1
prunus_persica	Prupe.8G134300_v2.0.a1 Prupe.8G134300_v2.0.a1
pyrus_communis	pycom05g08770
rosa_chinensis	RchiOBHm_Chr6g0255451
rosa_laevigata	RLG00000014836
rosa_multiflora	Rmu_sc0002360.1_g000009
rosa_roxburghii	Rroxscaffold_7G00209970
rosa_rugosa	Rorug05G0558900
rosa_samantha	Rh6AG074800 Rh6BG069400 Rh6CG068400 Rh6DG065200
rosa_wichuraiana	Rw6G006650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	407
AccB7I	CCANNNNNTGG	1 cut(s)	1192
AccBSI	CCGCTC	3 cut(s)	30, 312, 1384
AccII	CGCG	7 cut(s)	106, 122, 296, 330, 413, 467, 856
AccIII	TCCGGA	1 cut(s)	306
AclI	AACGTT	1 cut(s)	1653
AclWI	GGATC	3 cut(s)	408, 628, 1549
AcoI	YGGCCR	4 cut(s)	3, 450, 741, 1038
AcsI	RAATTY	1 cut(s)	1457
AcuI	CTGAAG	4 cut(s)	645, 1074, 1160, 1221
AcyI	GRCGYC	2 cut(s)	162, 408
AfaI	GTAC	1 cut(s)	1015
AfiI	CCNNNNNNNGG	6 cut(s)	351, 354, 384, 956, 1004, 1192
AflIII	ACRYGT	1 cut(s)	294
AgsI	TTSAA	6 cut(s)	361, 1046, 1154, 1550, 1744, 1772
AhdI	GACNNNNNGTC	1 cut(s)	1181
AjiI	CACGTC	1 cut(s)	9
AjnI	CCWGG	2 cut(s)	591, 1603
AjuI	GAANNNNNNNTTGG	2 cut(s)	1047, 1079
AleI	CACNNNNGTG	1 cut(s)	1005
AluBI	AGCT	9 cut(s)	193, 268, 539, 718, 726, 1084, 1241, 1247, 1563
AluI	AGCT	9 cut(s)	193, 268, 539, 718, 726, 1084, 1241, 1247, 1563
Alw21I	GWGCWC	3 cut(s)	270, 700, 1249
Alw26I	GTCTC	4 cut(s)	121, 803, 1168, 1301
AlwI	GGATC	3 cut(s)	408, 628, 1549
Ama87I	CYCGRG	2 cut(s)	260, 1406
Aor13HI	TCCGGA	1 cut(s)	306
AoxI	GGCC	5 cut(s)	3, 201, 450, 741, 1038
ApeKI	GCWGC	4 cut(s)	550, 782, 920, 1088
ApoI	RAATTY	1 cut(s)	1457
ArsI	GACNNNNNNTTYG	2 cut(s)	797, 829
AspLEI	GCGC	4 cut(s)	108, 124, 410, 856
AspS9I	GGNCC	1 cut(s)	1386
AsuC2I	CCSGG	2 cut(s)	205, 686
AsuHPI	GGTGA	3 cut(s)	484, 992, 1395
AvaI	CYCGRG	2 cut(s)	260, 1406
AvaII	GGWCC	1 cut(s)	1386
BalI	TGGCCA	3 cut(s)	5, 743, 1040
BanI	GGYRCC	1 cut(s)	407
BanII	GRGCYC	3 cut(s)	260, 270, 1249
BauI	CACGAG	1 cut(s)	699
Bbv12I	GWGCWC	3 cut(s)	270, 700, 1249
BbvCI	CCTCAGC	1 cut(s)	1122
BbvI	GCAGC	4 cut(s)	562, 769, 907, 1075
BccI	CCATC	4 cut(s)	1391, 1433, 1508, 1696
BceAI	ACGGC	2 cut(s)	465, 1043
BcgI	CGANNNNNNTGC	8 cut(s)	662, 696, 797, 831, 882, 916, 1070, 1104
BciT130I	CCWGG	2 cut(s)	593, 1605
BclI	TGATCA	1 cut(s)	1597
BcnI	CCSGG	2 cut(s)	205, 686
BcoDI	GTCTC	4 cut(s)	121, 803, 1168, 1301
BfaI	CTAG	5 cut(s)	1011, 1242, 1266, 1394, 1418
BfmI	CTRYAG	2 cut(s)	1095, 1585
BfoI	RGCGCY	1 cut(s)	411
BglII	AGATCT	1 cut(s)	279
BlpI	GCTNAGC	1 cut(s)	1353
Bme1390I	CCNGG	4 cut(s)	205, 593, 686, 1605
Bme18I	GGWCC	1 cut(s)	1386
BmeRI	GACNNNNNGTC	1 cut(s)	1181
BmeT110I	CYCGRG	2 cut(s)	260, 1406
BmgBI	CACGTC	1 cut(s)	9
BmgT120I	GGNCC	1 cut(s)	1386
BmiI	GGNNCC	4 cut(s)	409, 1069, 1116, 1521
BmrFI	CCNGG	4 cut(s)	205, 593, 686, 1605
BmsI	GCATC	3 cut(s)	23, 514, 868
BoxI	GACNNNNGTC	1 cut(s)	320
Bpu10I	CCTNAGC	5 cut(s)	254, 516, 642, 915, 1122
Bpu1102I	GCTNAGC	1 cut(s)	1353
BpuEI	CTTGAG	1 cut(s)	1772
BpuMI	CCSGG	2 cut(s)	205, 686
BsaHI	GRCGYC	2 cut(s)	162, 408
BsaI	GGTCTC	1 cut(s)	1301
BsaJI	CCNNGG	8 cut(s)	261, 465, 498, 591, 684, 1003, 1017, 1290
BsaWI	WCCGGW	1 cut(s)	306
Bsc4I	CCNNNNNNNGG	6 cut(s)	351, 354, 384, 956, 1004, 1192
Bse118I	RCCGGY	1 cut(s)	377
Bse1I	ACTGG	5 cut(s)	888, 1182, 1186, 1314, 1464
BseAI	TCCGGA	1 cut(s)	306
BseBI	CCWGG	2 cut(s)	593, 1605
BseDI	CCNNGG	8 cut(s)	261, 465, 498, 591, 684, 1003, 1017, 1290
BseGI	GGATG	5 cut(s)	14, 1425, 1519, 1723, 1762
BseLI	CCNNNNNNNGG	6 cut(s)	351, 354, 384, 956, 1004, 1192
BseMII	CTCAG	7 cut(s)	245, 408, 530, 929, 1113, 1152, 1381
BseNI	ACTGG	5 cut(s)	888, 1182, 1186, 1314, 1464
BseRI	GAGGAG	4 cut(s)	61, 278, 479, 1655
BseXI	GCAGC	4 cut(s)	562, 769, 907, 1075
BseYI	CCCAGC	1 cut(s)	1727
BsgI	GTGCAG	1 cut(s)	256
Bsh1236I	CGCG	7 cut(s)	106, 122, 296, 330, 413, 467, 856
BshFI	GGCC	5 cut(s)	5, 203, 452, 743, 1040
BshNI	GGYRCC	1 cut(s)	407
BsiHKAI	GWGCWC	3 cut(s)	270, 700, 1249
BsiHKCI	CYCGRG	2 cut(s)	260, 1406
BsiSI	CCGG	5 cut(s)	204, 307, 348, 378, 686
BslFI	GGGAC	2 cut(s)	328, 1398
BslI	CCNNNNNNNGG	6 cut(s)	351, 354, 384, 956, 1004, 1192
BsmAI	GTCTC	4 cut(s)	121, 803, 1168, 1301
BsmBI	CGTCTC	1 cut(s)	803
BsmFI	GGGAC	2 cut(s)	328, 1398
BsnI	GGCC	5 cut(s)	5, 203, 452, 743, 1040
Bso31I	GGTCTC	1 cut(s)	1301
BsoBI	CYCGRG	2 cut(s)	260, 1406
Bsp1286I	GDGCHC	4 cut(s)	260, 270, 700, 1249
Bsp13I	TCCGGA	1 cut(s)	306
Bsp143I	GATC	6 cut(s)	279, 400, 620, 691, 1541, 1597
Bsp1720I	GCTNAGC	1 cut(s)	1353
Bsp19I	CCATGG	2 cut(s)	498, 1017
BspANI	GGCC	5 cut(s)	5, 203, 452, 743, 1040
BspCNI	CTCAG	7 cut(s)	246, 409, 529, 928, 1114, 1153, 1380
BspEI	TCCGGA	1 cut(s)	306
BspFNI	CGCG	7 cut(s)	106, 122, 296, 330, 413, 467, 856
BspHI	TCATGA	1 cut(s)	1662
BspLI	GGNNCC	4 cut(s)	409, 1069, 1116, 1521
BspMAI	CTGCAG	2 cut(s)	1099, 1589
BspPI	GGATC	3 cut(s)	408, 628, 1549
BspQI	GCTCTTC	1 cut(s)	1374
BspT107I	GGYRCC	1 cut(s)	407
BspTNI	GGTCTC	1 cut(s)	1301
BsrBI	CCGCTC	3 cut(s)	30, 312, 1384
BsrFI	RCCGGY	1 cut(s)	377
BsrI	ACTGG	5 cut(s)	888, 1182, 1186, 1314, 1464
BssAI	RCCGGY	1 cut(s)	377
BssECI	CCNNGG	8 cut(s)	261, 465, 498, 591, 684, 1003, 1017, 1290
BssMI	GATC	6 cut(s)	279, 400, 620, 691, 1541, 1597
BssNI	GRCGYC	2 cut(s)	162, 408
BssSI	CACGAG	1 cut(s)	699
BssT1I	CCWWGG	4 cut(s)	498, 1003, 1017, 1290
Bst2BI	CACGAG	1 cut(s)	699
Bst2UI	CCWGG	2 cut(s)	593, 1605
Bst4CI	ACNGT	5 cut(s)	175, 216, 460, 940, 1481
Bst6I	CTCTTC	2 cut(s)	1374, 1767
BstACI	GRCGYC	2 cut(s)	162, 408
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstC8I	GCNNGC	7 cut(s)	379, 505, 639, 1082, 1086, 1695, 1733
BstDEI	CTNAG	9 cut(s)	254, 417, 516, 642, 915, 1122, 1161, 1353, 1367
BstDSI	CCRYGG	3 cut(s)	465, 498, 1017
BstF5I	GGATG	5 cut(s)	14, 1425, 1519, 1723, 1762
BstFNI	CGCG	7 cut(s)	106, 122, 296, 330, 413, 467, 856
BstH2I	RGCGCY	1 cut(s)	411
BstHHI	GCGC	4 cut(s)	108, 124, 410, 856
BstKTI	GATC	6 cut(s)	282, 403, 623, 694, 1544, 1600
BstMAI	GTCTC	4 cut(s)	121, 803, 1168, 1301
BstMBI	GATC	6 cut(s)	279, 400, 620, 691, 1541, 1597
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 449, 638, 655, 695, 920, 1094, 1103, 1703
BstNI	CCWGG	2 cut(s)	593, 1605
BstNSI	RCATGY	1 cut(s)	662
BstPAI	GACNNNNGTC	1 cut(s)	320
BstSCI	CCNGG	4 cut(s)	203, 591, 684, 1603
BstSFI	CTRYAG	2 cut(s)	1095, 1585
BstUI	CGCG	7 cut(s)	106, 122, 296, 330, 413, 467, 856
BstV1I	GCAGC	4 cut(s)	562, 769, 907, 1075
BstX2I	RGATCY	4 cut(s)	279, 400, 620, 1541
BstYI	RGATCY	4 cut(s)	279, 400, 620, 1541
BsuRI	GGCC	5 cut(s)	5, 203, 452, 743, 1040
BtgI	CCRYGG	3 cut(s)	465, 498, 1017
BtrI	CACGTC	1 cut(s)	9
BtsCI	GGATG	5 cut(s)	14, 1425, 1519, 1723, 1762
BtsI	GCAGTG	5 cut(s)	744, 777, 1092, 1210, 1347
Cac8I	GCNNGC	7 cut(s)	379, 505, 639, 1082, 1086, 1695, 1733
CciI	TCATGA	1 cut(s)	1662
CfoI	GCGC	4 cut(s)	108, 124, 410, 856
Cfr10I	RCCGGY	1 cut(s)	377
Cfr13I	GGNCC	1 cut(s)	1386
Cfr42I	CCGCGG	1 cut(s)	468
CseI	GACGC	2 cut(s)	170, 302
Csp6I	GTAC	1 cut(s)	1014
CspCI	CAANNNNNGTGG	4 cut(s)	693, 728, 1695, 1730
CviAII	CATG	8 cut(s)	476, 499, 601, 659, 977, 1018, 1282, 1663
CviQI	GTAC	1 cut(s)	1014
DdeI	CTNAG	9 cut(s)	254, 417, 516, 642, 915, 1122, 1161, 1353, 1367
DinI	GGCGCC	1 cut(s)	409
DpnI	GATC	6 cut(s)	281, 402, 622, 693, 1543, 1599
DpnII	GATC	6 cut(s)	279, 400, 620, 691, 1541, 1597
DriI	GACNNNNNGTC	1 cut(s)	1181
EaeI	YGGCCR	4 cut(s)	3, 450, 741, 1038
Eam1104I	CTCTTC	2 cut(s)	1374, 1767
Eam1105I	GACNNNNNGTC	1 cut(s)	1181
EarI	CTCTTC	2 cut(s)	1374, 1767
EciI	GGCGGA	1 cut(s)	1148
Ecl136II	GAGCTC	2 cut(s)	268, 1247
Eco130I	CCWWGG	4 cut(s)	498, 1003, 1017, 1290
Eco24I	GRGCYC	3 cut(s)	260, 270, 1249
Eco31I	GGTCTC	1 cut(s)	1301
Eco47I	GGWCC	1 cut(s)	1386
Eco53kI	GAGCTC	2 cut(s)	268, 1247
Eco57I	CTGAAG	4 cut(s)	645, 1074, 1160, 1221
Eco88I	CYCGRG	2 cut(s)	260, 1406
EcoICRI	GAGCTC	2 cut(s)	268, 1247
EcoRII	CCWGG	2 cut(s)	591, 1603
EcoT14I	CCWWGG	4 cut(s)	498, 1003, 1017, 1290
EcoT22I	ATGCAT	1 cut(s)	660
EcoT38I	GRGCYC	3 cut(s)	260, 270, 1249
EgeI	GGCGCC	1 cut(s)	409
EheI	GGCGCC	1 cut(s)	409
ErhI	CCWWGG	4 cut(s)	498, 1003, 1017, 1290
Esp3I	CGTCTC	1 cut(s)	803
FaeI	CATG	8 cut(s)	479, 502, 604, 662, 980, 1021, 1285, 1666
FalI	AAGNNNNNCTT	2 cut(s)	1068, 1100
FaqI	GGGAC	2 cut(s)	328, 1398
FatI	CATG	8 cut(s)	475, 498, 600, 658, 976, 1017, 1281, 1662
FauI	CCCGC	3 cut(s)	305, 344, 1126
FauNDI	CATATG	2 cut(s)	768, 1231
FbaI	TGATCA	1 cut(s)	1597
FokI	GGATG	4 cut(s)	1412, 1526, 1730, 1769
FriOI	GRGCYC	3 cut(s)	260, 270, 1249
FspBI	CTAG	5 cut(s)	1011, 1242, 1266, 1394, 1418
GlaI	GCGC	4 cut(s)	107, 123, 409, 855
GsaI	CCCAGC	1 cut(s)	1731
HaeII	RGCGCY	1 cut(s)	411
HaeIII	GGCC	5 cut(s)	5, 203, 452, 743, 1040
HapII	CCGG	5 cut(s)	204, 307, 348, 378, 686
HgaI	GACGC	2 cut(s)	170, 302
HhaI	GCGC	4 cut(s)	108, 124, 410, 856
Hin1I	GRCGYC	2 cut(s)	162, 408
Hin1II	CATG	8 cut(s)	479, 502, 604, 662, 980, 1021, 1285, 1666
Hin6I	GCGC	4 cut(s)	106, 122, 408, 854
HinP1I	GCGC	4 cut(s)	106, 122, 408, 854
HinfI	GANTC	7 cut(s)	341, 899, 1327, 1365, 1411, 1421, 1451
HpaII	CCGG	5 cut(s)	204, 307, 348, 378, 686
HphI	GGTGA	3 cut(s)	484, 992, 1395
Hpy166II	GTNNAC	4 cut(s)	238, 936, 1403, 1651
Hpy188I	TCNGA	5 cut(s)	447, 625, 1437, 1477, 1673
Hpy188III	TCNNGA	8 cut(s)	307, 370, 903, 926, 1250, 1369, 1394, 1663
Hpy8I	GTNNAC	4 cut(s)	238, 936, 1403, 1651
Hpy99I	CGWCG	6 cut(s)	164, 175, 296, 451, 464, 809
HpyAV	CCTTC	5 cut(s)	100, 313, 355, 1025, 1562
HpyCH4III	ACNGT	5 cut(s)	175, 216, 460, 940, 1481
HpyCH4IV	ACGT	4 cut(s)	8, 510, 812, 1653
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 449, 638, 655, 695, 920, 1094, 1103, 1703
HpyF3I	CTNAG	9 cut(s)	254, 417, 516, 642, 915, 1122, 1161, 1353, 1367
HpySE526I	ACGT	4 cut(s)	8, 510, 812, 1653
Hsp92I	GRCGYC	2 cut(s)	162, 408
Hsp92II	CATG	8 cut(s)	479, 502, 604, 662, 980, 1021, 1285, 1666
HspAI	GCGC	4 cut(s)	106, 122, 408, 854
KasI	GGCGCC	1 cut(s)	407
Kpn2I	TCCGGA	1 cut(s)	306
KroI	GCCGGC	1 cut(s)	377
KroNI	GCCGGC	1 cut(s)	379
Ksp22I	TGATCA	1 cut(s)	1597
KspI	CCGCGG	1 cut(s)	468
Kzo9I	GATC	6 cut(s)	279, 400, 620, 691, 1541, 1597
LguI	GCTCTTC	1 cut(s)	1374
LmnI	GCTCC	7 cut(s)	35, 265, 309, 448, 715, 1067, 1252
Lsp1109I	GCAGC	4 cut(s)	562, 769, 907, 1075
LweI	GCATC	3 cut(s)	23, 514, 868
MaeI	CTAG	5 cut(s)	1011, 1242, 1266, 1394, 1418
MaeII	ACGT	4 cut(s)	8, 510, 812, 1653
MaeIII	GTNAC	1 cut(s)	985
MalI	GATC	6 cut(s)	281, 402, 622, 693, 1543, 1599
MbiI	CCGCTC	3 cut(s)	30, 312, 1384
MboI	GATC	6 cut(s)	279, 400, 620, 691, 1541, 1597
MboII	GAAGA	9 cut(s)	289, 832, 838, 887, 1225, 1265, 1391, 1686, 1784
MfeI	CAATTG	2 cut(s)	705, 1707
MflI	RGATCY	4 cut(s)	279, 400, 620, 1541
MhlI	GDGCHC	4 cut(s)	260, 270, 700, 1249
MlsI	TGGCCA	3 cut(s)	5, 743, 1040
MluCI	AATT	7 cut(s)	705, 840, 906, 1047, 1457, 1677, 1707
MluI	ACGCGT	1 cut(s)	294
MluNI	TGGCCA	3 cut(s)	5, 743, 1040
Mly113I	GGCGCC	1 cut(s)	408
MlyI	GAGTC	4 cut(s)	350, 1359, 1415, 1420
MmeI	TCCRAC	6 cut(s)	470, 598, 1045, 1519, 1603, 1651
Mox20I	TGGCCA	3 cut(s)	5, 743, 1040
Mph1103I	ATGCAT	1 cut(s)	660
MroI	TCCGGA	1 cut(s)	306
MroNI	GCCGGC	1 cut(s)	377
MscI	TGGCCA	3 cut(s)	5, 743, 1040
MseI	TTAA	5 cut(s)	218, 438, 534, 1073, 1509
MslI	CAYNNNNRTG	2 cut(s)	497, 1005
Msp20I	TGGCCA	3 cut(s)	5, 743, 1040
MspA1I	CMGCKG	2 cut(s)	467, 1121
MspI	CCGG	5 cut(s)	204, 307, 348, 378, 686
MspR9I	CCNGG	4 cut(s)	205, 593, 686, 1605
MunI	CAATTG	2 cut(s)	705, 1707
MvaI	CCWGG	2 cut(s)	593, 1605
MvnI	CGCG	7 cut(s)	106, 122, 296, 330, 413, 467, 856
MwoI	GCNNNNNNNGC	9 cut(s)	11, 449, 638, 655, 695, 920, 1094, 1103, 1703
NaeI	GCCGGC	1 cut(s)	379
NarI	GGCGCC	1 cut(s)	408
NciI	CCSGG	2 cut(s)	205, 686
NcoI	CCATGG	2 cut(s)	498, 1017
NdeI	CATATG	2 cut(s)	768, 1231
NdeII	GATC	6 cut(s)	279, 400, 620, 691, 1541, 1597
NgoMIV	GCCGGC	1 cut(s)	377
NlaIII	CATG	8 cut(s)	479, 502, 604, 662, 980, 1021, 1285, 1666
NlaIV	GGNNCC	4 cut(s)	409, 1069, 1116, 1521
NmeAIII	GCCGAG	5 cut(s)	125, 150, 174, 211, 613
NsiI	ATGCAT	1 cut(s)	660
NspI	RCATGY	1 cut(s)	662
OliI	CACNNNNGTG	1 cut(s)	1005
PagI	TCATGA	1 cut(s)	1662
PciSI	GCTCTTC	1 cut(s)	1374
PdiI	GCCGGC	1 cut(s)	379
PfeI	GAWTC	3 cut(s)	899, 1327, 1451
PflMI	CCANNNNNTGG	1 cut(s)	1192
PleI	GAGTC	4 cut(s)	349, 1359, 1415, 1419
PluTI	GGCGCC	1 cut(s)	411
PpsI	GAGTC	4 cut(s)	349, 1359, 1415, 1419
PshAI	GACNNNNGTC	1 cut(s)	320
Psp124BI	GAGCTC	2 cut(s)	270, 1249
Psp1406I	AACGTT	1 cut(s)	1653
Psp6I	CCWGG	2 cut(s)	591, 1603
PspFI	CCCAGC	1 cut(s)	1727
PspGI	CCWGG	2 cut(s)	591, 1603
PspN4I	GGNNCC	4 cut(s)	409, 1069, 1116, 1521
PspPI	GGNCC	1 cut(s)	1386
PstI	CTGCAG	2 cut(s)	1099, 1589
PsuI	RGATCY	4 cut(s)	279, 400, 620, 1541
RsaI	GTAC	1 cut(s)	1015
RsaNI	GTAC	1 cut(s)	1014
RseI	CAYNNNNRTG	2 cut(s)	497, 1005
SacI	GAGCTC	2 cut(s)	270, 1249
SacII	CCGCGG	1 cut(s)	468
SapI	GCTCTTC	1 cut(s)	1374
SaqAI	TTAA	5 cut(s)	218, 438, 534, 1073, 1509
Sau3AI	GATC	6 cut(s)	279, 400, 620, 691, 1541, 1597
Sau96I	GGNCC	1 cut(s)	1386
SchI	GAGTC	4 cut(s)	350, 1359, 1415, 1420
ScrFI	CCNGG	4 cut(s)	205, 593, 686, 1605
SduI	GDGCHC	4 cut(s)	260, 270, 700, 1249
SfaNI	GCATC	3 cut(s)	23, 514, 868
SfcI	CTRYAG	2 cut(s)	1095, 1585
SfoI	GGCGCC	1 cut(s)	409
Sfr303I	CCGCGG	1 cut(s)	468
SgrBI	CCGCGG	1 cut(s)	468
SinI	GGWCC	1 cut(s)	1386
SmiMI	CAYNNNNRTG	2 cut(s)	497, 1005
SmlI	CTYRAG	1 cut(s)	1787
SmoI	CTYRAG	1 cut(s)	1787
Sse9I	AATT	7 cut(s)	705, 840, 906, 1047, 1457, 1677, 1707
SspDI	GGCGCC	1 cut(s)	407
SspMI	CTAG	5 cut(s)	1011, 1242, 1266, 1394, 1418
SstI	GAGCTC	2 cut(s)	270, 1249
StyD4I	CCNGG	4 cut(s)	203, 591, 684, 1603
StyI	CCWWGG	4 cut(s)	498, 1003, 1017, 1290
TaaI	ACNGT	5 cut(s)	175, 216, 460, 940, 1481
TaiI	ACGT	4 cut(s)	11, 513, 815, 1656
TaqI	TCGA	4 cut(s)	159, 729, 942, 1276
TasI	AATT	7 cut(s)	705, 840, 906, 1047, 1457, 1677, 1707
TauI	GCSGC	8 cut(s)	111, 114, 122, 152, 330, 413, 467, 470
TfiI	GAWTC	3 cut(s)	899, 1327, 1451
Tru1I	TTAA	5 cut(s)	218, 438, 534, 1073, 1509
Tru9I	TTAA	5 cut(s)	218, 438, 534, 1073, 1509
TseI	GCWGC	4 cut(s)	550, 782, 920, 1088
TspDTI	ATGAA	4 cut(s)	745, 839, 1218, 1651
Van91I	CCANNNNNTGG	1 cut(s)	1192
VpaK11BI	GGWCC	1 cut(s)	1386
XapI	RAATTY	1 cut(s)	1457
XbaI	TCTAGA	1 cut(s)	1393
XceI	RCATGY	1 cut(s)	662
XspI	CTAG	5 cut(s)	1011, 1242, 1266, 1394, 1418
Zsp2I	ATGCAT	1 cut(s)	660

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.