FvH4_2g05540

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016747
Physical Location & Seq
Forward (+)
4572873 .. 4574228
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05540.t1

Sequence Viewer

Length: 1236 bp
ATGGGAAGCTTCTTTTTACTGTCTGTGCGCCTGCTTCATGCCTTCTTCATCATCCTTCCCTGTTTCTTCAATACTGTAATCTGCACAAACCATAAGACTTCATTAACATTACCTTCATTACCTCCATTACCTCCATTAATCCCCCAACTCCTGAATTTCGCAGACCAAAGACTCTCAGTGGTTTACCCGATTATACAATCCTTCAAGAACATCATCACTTCAGATCCATTAGGCATCACCAAAACCTGGGTTGGCGCTGACATATGCAACTACAAGGGCTTCTACTGTGACATCCCACCGGACAATGCCACCGCCACTGCCATCGCCTCCATAGATTTCAACGGCTTCCAACTCAGTGCTCCAACTCTTGACGGCTTCATAGATCAACTTCCTGACCTTGCCCTCTTTCATGCCAACTCCAACAACTTCTCAGGTATCCTAACACCAAAAATTGCAAATCTCCAATACCTGTATGAGCTTGACATAAGCAACAACAACTTCTTTGGCCCTTTTCCCACGGCAGTACTCACCATGAATAGCCTTTCATTCTTGGACATTCGCTACAACTCTTTTACCGGATCCATACCACCCGAAATTTTCAAGCAAACCCTGGACGTTCTCTTCCTCAACAACAACAATTTCATGCAAAGCCTCCCTGATAACCTTGGTGCTACTTCAGCGCTCTATCTCACCTTAGCCAACAACAAATTTACTGGCTCCATTCCGAAAAGCATCGGAAGTGCTTCACCAACTTTGGTAGAAGCGCTGCTCTTGAACAATTTGTTGACCGGTTGTATCCCTTATGAACTAGGGTTTCTCAGAGATGCCACTGTGTTTGATGCCGGGAACAACCAGTTGACCGGCCCTTTGCCGTGTTCATTGGGATGCTTGGACAAGATAGAACAGCTTAACTTTGCTGATAACTTTCTCTATGGACGTGTGCCGGAGGTGCTCTGTGAACTAGGGAATTTGGCGAATCTATCTCTGTCGAACAACTACTTCACTGGAGTAGGAACAGCGTGTAGAAAGCTGATAGAAAAGGGTGTCCTTGACATAAGGAACAATTGCATTCAGGATCTTGCAGATCAAAGATCAGCGGAGGAATGTGCCTTGTTCTTCTTGCACCCTAAAAGCTGCCTGCACCCTGAGACATATAGTCTAATTCCTTGTAAGGCTCCACCAAAATCAAAGACGAAATCGTCTCCATATTCTGCAGTTCCCAAACAGAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

412

Amino Acids

45.15

Weight (kDa)

5.56

Isoelectric Point (pI)

30.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016747)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05540
malus_domestica MD05G1086600.v1.1
prunus_persica Prupe.8G131300_v2.0.a1
pyrus_communis pycom05g08600
rosa_chinensis RchiOBHm_Chr6g0255851
rosa_laevigata RLG00000014811
rosa_multiflora Rmu_sc0001163.1_g000035
rosa_roxburghii Rroxscaffold_7G00209590
rosa_rugosa Rorug05G0561700
rosa_samantha Rh6AG080000 Rh6BG072900 Rh6CG070900 Rh6DG068200
rosa_wichuraiana Rw6G006900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1198
AccB7I CCANNNNNTGG 1 cut(s) 246
AciI CCGC 2 cut(s) 312, 1097
AclWI GGATC 4 cut(s) 218, 573, 586, 1083
AcsI RAATTY 4 cut(s) 154, 594, 707, 967
AcuI CTGAAG 2 cut(s) 204, 660
AfaI GTAC 1 cut(s) 525
AfeI AGCGCT 2 cut(s) 681, 765
AfiI CCNNNNNNNGG 1 cut(s) 246
AflIII ACRYGT 1 cut(s) 937
AgeI ACCGGT 1 cut(s) 788
AgsI TTSAA 5 cut(s) 70, 205, 340, 601, 775
AhdI GACNNNNNGTC 1 cut(s) 1155
AjiI CACGTC 1 cut(s) 938
AjnI CCWGG 2 cut(s) 245, 609
AjuI GAANNNNNNNTTGG 1 cut(s) 1214
AluBI AGCT 5 cut(s) 9, 478, 907, 1030, 1134
AluI AGCT 5 cut(s) 9, 478, 907, 1030, 1134
Alw21I GWGCWC 2 cut(s) 361, 954
Alw26I GTCTC 2 cut(s) 1142, 1206
AlwI GGATC 4 cut(s) 218, 573, 586, 1083
Aor51HI AGCGCT 2 cut(s) 681, 765
AoxI GGCC 2 cut(s) 505, 862
ApeKI GCWGC 2 cut(s) 766, 1134
ApoI RAATTY 4 cut(s) 154, 594, 707, 967
ArsI GACNNNNNNTTYG 2 cut(s) 850, 882
AseI ATTAAT 1 cut(s) 137
AsiGI ACCGGT 1 cut(s) 788
Asp700I GAANNNNTTC 1 cut(s) 742
AspLEI GCGC 4 cut(s) 30, 257, 682, 766
AspS9I GGNCC 2 cut(s) 506, 863
AsuC2I CCSGG 1 cut(s) 844
AsuHPI GGTGA 4 cut(s) 229, 520, 682, 738
BamHI GGATCC 1 cut(s) 578
Bbv12I GWGCWC 2 cut(s) 361, 954
BbvI GCAGC 2 cut(s) 753, 1121
BccI CCATC 1 cut(s) 329
BceAI ACGGC 4 cut(s) 358, 388, 534, 856
BciT130I CCWGG 2 cut(s) 247, 611
BciVI GTATCC 2 cut(s) 446, 806
BcnI CCSGG 1 cut(s) 844
BcoDI GTCTC 2 cut(s) 1142, 1206
BfaI CTAG 2 cut(s) 809, 962
BfmI CTRYAG 1 cut(s) 1212
BfoI RGCGCY 3 cut(s) 258, 683, 767
BfuI GTATCC 2 cut(s) 446, 806
BisI GCNGC 2 cut(s) 767, 1135
BlsI GCNGC 2 cut(s) 768, 1136
BmcAI AGTACT 1 cut(s) 525
Bme1390I CCNGG 3 cut(s) 247, 611, 844
BmeRI GACNNNNNGTC 1 cut(s) 1155
BmgBI CACGTC 1 cut(s) 938
BmgT120I GGNCC 2 cut(s) 506, 863
BmiI GGNNCC 3 cut(s) 580, 718, 1176
BmrFI CCNGG 3 cut(s) 247, 611, 844
BmsI GCATC 5 cut(s) 243, 741, 814, 829, 875
BpmI CTGGAG 1 cut(s) 1026
Bpu10I CCTNAGC 1 cut(s) 694
BpuMI CCSGG 1 cut(s) 844
BsaJI CCNNGG 4 cut(s) 246, 516, 609, 664
BsaWI WCCGGW 3 cut(s) 298, 575, 788
Bsc4I CCNNNNNNNGG 1 cut(s) 246
Bse118I RCCGGY 2 cut(s) 788, 860
Bse1I ACTGG 3 cut(s) 718, 853, 1009
BseBI CCWGG 2 cut(s) 247, 611
BseDI CCNNGG 4 cut(s) 246, 516, 609, 664
BseGI GGATG 3 cut(s) 51, 291, 890
BseLI CCNNNNNNNGG 1 cut(s) 246
BseMII CTCAG 5 cut(s) 189, 367, 444, 832, 1137
BseNI ACTGG 3 cut(s) 718, 853, 1009
BseXI GCAGC 2 cut(s) 753, 1121
BsgI GTGCAG 2 cut(s) 67, 1124
BshFI GGCC 2 cut(s) 507, 864
BshTI ACCGGT 1 cut(s) 788
BsiHKAI GWGCWC 2 cut(s) 361, 954
BsiSI CCGG 6 cut(s) 299, 576, 789, 843, 861, 944
BslI CCNNNNNNNGG 1 cut(s) 246
BsmAI GTCTC 2 cut(s) 1142, 1206
BsmBI CGTCTC 1 cut(s) 1206
BsmI GAATGC 1 cut(s) 1068
BsnI GGCC 2 cut(s) 507, 864
Bsp1286I GDGCHC 2 cut(s) 361, 954
Bsp143I GATC 6 cut(s) 223, 382, 578, 1075, 1084, 1091
BspACI CCGC 2 cut(s) 312, 1097
BspANI GGCC 2 cut(s) 507, 864
BspCNI CTCAG 5 cut(s) 188, 366, 443, 831, 1138
BspLI GGNNCC 3 cut(s) 580, 718, 1176
BspMAI CTGCAG 1 cut(s) 1216
BspPI GGATC 4 cut(s) 218, 573, 586, 1083
BsrFI RCCGGY 2 cut(s) 788, 860
BsrI ACTGG 3 cut(s) 718, 853, 1009
BssAI RCCGGY 2 cut(s) 788, 860
BssECI CCNNGG 4 cut(s) 246, 516, 609, 664
BssMI GATC 6 cut(s) 223, 382, 578, 1075, 1084, 1091
BssT1I CCWWGG 1 cut(s) 664
Bst2UI CCWGG 2 cut(s) 247, 611
Bst4CI ACNGT 4 cut(s) 21, 76, 287, 832
Bst6I CTCTTC 1 cut(s) 626
BstC8I GCNNGC 2 cut(s) 32, 1139
BstDEI CTNAG 6 cut(s) 175, 353, 430, 694, 818, 1146
BstDSI CCRYGG 1 cut(s) 516
BstF5I GGATG 3 cut(s) 51, 291, 890
BstH2I RGCGCY 3 cut(s) 258, 683, 767
BstHHI GCGC 4 cut(s) 30, 257, 682, 766
BstKTI GATC 6 cut(s) 226, 385, 581, 1078, 1087, 1094
BstMAI GTCTC 2 cut(s) 1142, 1206
BstMBI GATC 6 cut(s) 223, 382, 578, 1075, 1084, 1091
BstMWI GCNNNNNNNGC 2 cut(s) 677, 949
BstNI CCWGG 2 cut(s) 247, 611
BstSCI CCNGG 3 cut(s) 245, 609, 842
BstSFI CTRYAG 1 cut(s) 1212
BstV1I GCAGC 2 cut(s) 753, 1121
BstX2I RGATCY 3 cut(s) 223, 578, 1075
BstYI RGATCY 3 cut(s) 223, 578, 1075
BsuI GTATCC 2 cut(s) 446, 806
BsuRI GGCC 2 cut(s) 507, 864
BtgI CCRYGG 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 307
BtrI CACGTC 1 cut(s) 938
BtsCI GGATG 3 cut(s) 51, 291, 890
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 5 cut(s) 183, 315, 361, 828, 1002
Cac8I GCNNGC 2 cut(s) 32, 1139
CfoI GCGC 4 cut(s) 30, 257, 682, 766
Cfr10I RCCGGY 2 cut(s) 788, 860
Cfr13I GGNCC 2 cut(s) 506, 863
Csp6I GTAC 1 cut(s) 524
CspAI ACCGGT 1 cut(s) 788
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviAII CATG 4 cut(s) 38, 410, 532, 643
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 6 cut(s) 175, 353, 430, 694, 818, 1146
DpnI GATC 6 cut(s) 225, 384, 580, 1077, 1086, 1093
DpnII GATC 6 cut(s) 223, 382, 578, 1075, 1084, 1091
DrdI GACNNNNNNGTC 1 cut(s) 1198
DriI GACNNNNNGTC 1 cut(s) 1155
DseDI GACNNNNNNGTC 1 cut(s) 1198
Eam1104I CTCTTC 1 cut(s) 626
Eam1105I GACNNNNNGTC 1 cut(s) 1155
EarI CTCTTC 1 cut(s) 626
Eco130I CCWWGG 1 cut(s) 664
Eco47III AGCGCT 2 cut(s) 681, 765
Eco57I CTGAAG 2 cut(s) 204, 660
EcoRII CCWGG 2 cut(s) 245, 609
EcoT14I CCWWGG 1 cut(s) 664
ErhI CCWWGG 1 cut(s) 664
Esp3I CGTCTC 1 cut(s) 1206
FaeI CATG 4 cut(s) 41, 413, 535, 646
FatI CATG 4 cut(s) 37, 409, 531, 642
FauNDI CATATG 1 cut(s) 263
Fnu4HI GCNGC 2 cut(s) 767, 1135
FokI GGATG 3 cut(s) 38, 278, 897
Fsp4HI GCNGC 2 cut(s) 767, 1135
FspBI CTAG 2 cut(s) 809, 962
GlaI GCGC 4 cut(s) 29, 256, 681, 765
GluI GCNGC 2 cut(s) 767, 1135
GsuI CTGGAG 1 cut(s) 1026
HaeII RGCGCY 3 cut(s) 258, 683, 767
HaeIII GGCC 2 cut(s) 507, 864
HapII CCGG 6 cut(s) 299, 576, 789, 843, 861, 944
HhaI GCGC 4 cut(s) 30, 257, 682, 766
Hin1II CATG 4 cut(s) 41, 413, 535, 646
Hin6I GCGC 4 cut(s) 28, 255, 680, 764
HinP1I GCGC 4 cut(s) 28, 255, 680, 764
HincII GTYRAC 2 cut(s) 786, 858
HindII GTYRAC 2 cut(s) 786, 858
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 3 cut(s) 171, 976, 1230
HpaII CCGG 6 cut(s) 299, 576, 789, 843, 861, 944
HphI GGTGA 4 cut(s) 229, 520, 682, 738
Hpy166II GTNNAC 4 cut(s) 184, 786, 858, 959
Hpy188I TCNGA 5 cut(s) 223, 726, 737, 821, 1235
Hpy188III TCNNGA 6 cut(s) 151, 205, 368, 392, 772, 1073
Hpy8I GTNNAC 4 cut(s) 184, 786, 858, 959
HpyAV CCTTC 4 cut(s) 52, 65, 123, 211
HpyCH4III ACNGT 4 cut(s) 21, 76, 287, 832
HpyCH4IV ACGT 2 cut(s) 615, 937
HpyCH4V TGCA 9 cut(s) 84, 267, 455, 646, 1068, 1082, 1123, 1141, 1214
HpyF10VI GCNNNNNNNGC 2 cut(s) 677, 949
HpyF3I CTNAG 6 cut(s) 175, 353, 430, 694, 818, 1146
HpySE526I ACGT 2 cut(s) 615, 937
Hsp92II CATG 4 cut(s) 41, 413, 535, 646
HspAI GCGC 4 cut(s) 28, 255, 680, 764
Kzo9I GATC 6 cut(s) 223, 382, 578, 1075, 1084, 1091
LmnI GCTCC 3 cut(s) 364, 722, 1180
Lsp1109I GCAGC 2 cut(s) 753, 1121
LweI GCATC 5 cut(s) 243, 741, 814, 829, 875
MaeI CTAG 2 cut(s) 809, 962
MaeII ACGT 2 cut(s) 615, 937
MaeIII GTNAC 1 cut(s) 287
MalI GATC 6 cut(s) 225, 384, 580, 1077, 1086, 1093
MboI GATC 6 cut(s) 223, 382, 578, 1075, 1084, 1091
MboII GAAGA 4 cut(s) 37, 58, 613, 1108
MfeI CAATTG 1 cut(s) 1063
MflI RGATCY 3 cut(s) 223, 578, 1075
MhlI GDGCHC 2 cut(s) 361, 954
MluCI AATT 9 cut(s) 154, 450, 594, 637, 707, 778, 967, 1063, 1161
MlyI GAGTC 1 cut(s) 165
MmeI TCCRAC 3 cut(s) 373, 386, 444
MnlI CCTC 8 cut(s) 132, 141, 337, 413, 635, 662, 940, 1093
MroXI GAANNNNTTC 1 cut(s) 742
MseI TTAA 3 cut(s) 104, 137, 909
MslI CAYNNNNRTG 1 cut(s) 883
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 6 cut(s) 299, 576, 789, 843, 861, 944
MspR9I CCNGG 3 cut(s) 247, 611, 844
MunI CAATTG 1 cut(s) 1063
Mva1269I GAATGC 1 cut(s) 1068
MvaI CCWGG 2 cut(s) 247, 611
MwoI GCNNNNNNNGC 2 cut(s) 677, 949
NciI CCSGG 1 cut(s) 844
NdeI CATATG 1 cut(s) 263
NdeII GATC 6 cut(s) 223, 382, 578, 1075, 1084, 1091
NlaIII CATG 4 cut(s) 41, 413, 535, 646
NlaIV GGNNCC 3 cut(s) 580, 718, 1176
NmuCI GTSAC 1 cut(s) 287
PctI GAATGC 1 cut(s) 1068
PdmI GAANNNNTTC 1 cut(s) 742
PfeI GAWTC 2 cut(s) 976, 1230
PflMI CCANNNNNTGG 1 cut(s) 246
PinAI ACCGGT 1 cut(s) 788
PkrI GCNGC 2 cut(s) 768, 1136
PleI GAGTC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 165
PshBI ATTAAT 1 cut(s) 137
Psp6I CCWGG 2 cut(s) 245, 609
PspGI CCWGG 2 cut(s) 245, 609
PspN4I GGNNCC 3 cut(s) 580, 718, 1176
PspPI GGNCC 2 cut(s) 506, 863
PstI CTGCAG 1 cut(s) 1216
PsuI RGATCY 3 cut(s) 223, 578, 1075
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
RseI CAYNNNNRTG 1 cut(s) 883
SaqAI TTAA 3 cut(s) 104, 137, 909
SatI GCNGC 2 cut(s) 767, 1135
Sau3AI GATC 6 cut(s) 223, 382, 578, 1075, 1084, 1091
Sau96I GGNCC 2 cut(s) 506, 863
ScaI AGTACT 1 cut(s) 525
SchI GAGTC 1 cut(s) 165
ScrFI CCNGG 3 cut(s) 247, 611, 844
SduI GDGCHC 2 cut(s) 361, 954
SfaNI GCATC 5 cut(s) 243, 741, 814, 829, 875
SfcI CTRYAG 1 cut(s) 1212
SmiMI CAYNNNNRTG 1 cut(s) 883
Sse9I AATT 9 cut(s) 154, 450, 594, 637, 707, 778, 967, 1063, 1161
SsiI CCGC 2 cut(s) 312, 1097
SspMI CTAG 2 cut(s) 809, 962
StyD4I CCNGG 3 cut(s) 245, 609, 842
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 4 cut(s) 21, 76, 287, 832
TaiI ACGT 2 cut(s) 618, 940
TaqI TCGA 1 cut(s) 989
TasI AATT 9 cut(s) 154, 450, 594, 637, 707, 778, 967, 1063, 1161
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 2 cut(s) 976, 1230
Tru1I TTAA 3 cut(s) 104, 137, 909
Tru9I TTAA 3 cut(s) 104, 137, 909
TscAI CASTG 5 cut(s) 183, 322, 361, 835, 1009
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 2 cut(s) 766, 1134
Tsp45I GTSAC 1 cut(s) 287
TspRI CASTG 5 cut(s) 183, 322, 361, 835, 1009
Van91I CCANNNNNTGG 1 cut(s) 246
VspI ATTAAT 1 cut(s) 137
XapI RAATTY 4 cut(s) 154, 594, 707, 967
XmnI GAANNNNTTC 1 cut(s) 742
XspI CTAG 2 cut(s) 809, 962
ZrmI AGTACT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.