MD05G1086600.v1.1

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016747
Physical Location & Seq
Reverse (-)
18461856 .. 18463328
1473 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1086600.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGGAAATTTCATTTTACTAAACATTGAACATCTGTTTCCTGCCTTCTTCATCATCCTAATTCCTTGTTTCTTTGATGGTGTAATTTGCACAGACTCAGAAAGCTCATCAATCTTACCTCCTTTAATCCCCCTTCCCCCATTACCTCCTTTAGTCCCCCTTCCCCCGTTACCTCCATTAATCCCCCAACTTCTGAACTTCGCAGACCAAAGACTAGCTCTTGTTTACCCTATAATCATGACCTTTAGGAACATGATCATTTCTGACCCATTAGGCATCACTCAAACCTGGGTTGGCTCCGACATATGCAACTACAAGGGTTTCTACTGTGATAACCCTCCAGACAATAAAAGCGCCACTACACTTGCCTCCATAGATTTCAATGGCTTCCAGCTCAGTGCTCCCACTCTTGACGGCTTCATCGATCAACTTCCTGACCTAGCACTTTTTCATGCTAACTCCAATAATTTCTCAGGTATCGTTTCTCCAAAAATTGCAAATCTCCAATACCTCTATGAGCTTGACATAAGCAACAACAACTTCTCTGGCCCTTTTCCTACTGCTGTTCTCACCATGAATAGTCTATCATTCTTGGACATCCGCTACAATTCCTTCACTGGGTCCGTACCACCCCAAATTTTCAAGCAAACCCTAGACGTCCTTTTCATTAATAACAACAATTTCATACAAAAACTCCCGGACAACCTTGGAGCTACACGAGCCCTTTATCTCACTTTAGCCAACAACAGATTCACCGGCCCAATCCCAAAAAGCATCGGCAGCGCTTCATCATCGTTGAAAGAAGTACTATTATTGAACAATTTGATCACAGGTTGCATCCCTTATGAACTAGGGTTTTTAAGAGATGCAACACTTTTTGATGCCGGAAACAACCTATTGACCGGCCCGCTACCCTGTTCATTGGGATGCTTGGACAAGATGGAGGAACTTAATTTTGCCGGAAACTTACTGTACGGAAAGGTGCCGGAGGTGGTGTGCGAACTAGGAAATTTGGTCAATTTATCGCTGTCCAACAACTATTTCACGTCGGTCGGGCCTATTTGTAGGCAGCTGATAAAAAATGGGGTTCTTGACTTGAGGAAGAACTGTATTCAGCATCTTGCTGATCAAAGATCAGTGGAGGAGTGTGCCTTGTTTTTCGTGAAGCCTAGAAGTTGCCCGGACCCGTCGACGTTTAGTTTACTGCCTTGTAAGGTTCCACCAAAATCGAAGGGGCATTTGGTGTCTGATAATGCTGCTTTTTCGAAGCATAGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

46.79

Weight (kDa)

5.4

Isoelectric Point (pI)

32.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016747)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05540
malus_domestica MD05G1086600.v1.1
prunus_persica Prupe.8G131300_v2.0.a1
pyrus_communis pycom05g08600
rosa_chinensis RchiOBHm_Chr6g0255851
rosa_laevigata RLG00000014811
rosa_multiflora Rmu_sc0001163.1_g000035
rosa_roxburghii Rroxscaffold_7G00209590
rosa_rugosa Rorug05G0561700
rosa_samantha Rh6AG080000 Rh6BG072900 Rh6CG070900 Rh6DG068200
rosa_wichuraiana Rw6G006900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 660
AccB1I GGYRCC 1 cut(s) 982
AccI GTMKAC 1 cut(s) 1190
AciI CCGC 2 cut(s) 601, 908
AcsI RAATTY 3 cut(s) 7, 636, 1009
AcyI GRCGYC 1 cut(s) 657
AfaI GTAC 3 cut(s) 627, 807, 974
AfeI AGCGCT 1 cut(s) 784
AfiI CCNNNNNNNGG 1 cut(s) 618
AgsI TTSAA 5 cut(s) 29, 382, 643, 799, 817
AjiI CACGTC 1 cut(s) 1047
AjnI CCWGG 1 cut(s) 287
AjuI GAANNNNNNNTTGG 6 cut(s) 734, 766, 1025, 1057, 1223, 1255
AluBI AGCT 6 cut(s) 105, 218, 394, 520, 713, 1072
AluI AGCT 6 cut(s) 105, 218, 394, 520, 713, 1072
Alw21I GWGCWC 1 cut(s) 403
Aor51HI AGCGCT 1 cut(s) 784
AoxI GGCC 4 cut(s) 547, 757, 904, 1055
ApeKI GCWGC 3 cut(s) 780, 1069, 1256
ApoI RAATTY 3 cut(s) 7, 636, 1009
AseI ATTAAT 2 cut(s) 179, 669
AspLEI GCGC 2 cut(s) 356, 785
AspS9I GGNCC 6 cut(s) 548, 621, 758, 905, 1055, 1183
AsuC2I CCSGG 2 cut(s) 698, 1181
AsuHPI GGTGA 2 cut(s) 562, 745
AsuII TTCGAA 1 cut(s) 1265
AvaII GGWCC 2 cut(s) 621, 1183
BanI GGYRCC 1 cut(s) 982
BanII GRGCYC 1 cut(s) 724
BauI CACGAG 1 cut(s) 717
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 3 cut(s) 792, 1081, 1243
BccI CCATC 2 cut(s) 71, 934
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 289
BclI TGATCA 3 cut(s) 255, 825, 1126
BcnI CCSGG 2 cut(s) 698, 1181
BfaI CTAG 6 cut(s) 215, 440, 653, 851, 1004, 1170
BfoI RGCGCY 2 cut(s) 357, 786
BisI GCNGC 3 cut(s) 781, 1070, 1257
BlsI GCNGC 3 cut(s) 782, 1071, 1258
BmcAI AGTACT 1 cut(s) 807
Bme1390I CCNGG 3 cut(s) 289, 698, 1181
Bme18I GGWCC 2 cut(s) 621, 1183
BmgBI CACGTC 1 cut(s) 1047
BmgT120I GGNCC 6 cut(s) 548, 621, 758, 905, 1055, 1183
BmiI GGNNCC 5 cut(s) 298, 622, 984, 1185, 1218
BmrFI CCNGG 3 cut(s) 289, 698, 1181
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 7 cut(s) 285, 783, 846, 856, 871, 917, 1126
BmuI ACTGGG 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 324
Bpu14I TTCGAA 1 cut(s) 1265
BpuEI CTTGAG 1 cut(s) 1117
BpuMI CCSGG 2 cut(s) 698, 1181
Bsa29I ATCGAT 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 657
BsaJI CCNNGG 2 cut(s) 288, 706
BsaXI ACNNNNNCTCC 4 cut(s) 678, 708, 980, 1010
Bsc4I CCNNNNNNNGG 1 cut(s) 618
Bse118I RCCGGY 2 cut(s) 755, 902
Bse1I ACTGG 1 cut(s) 622
BseBI CCWGG 1 cut(s) 289
BseCI ATCGAT 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 288, 706
BseGI GGATG 4 cut(s) 54, 597, 837, 932
BseLI CCNNNNNNNGG 1 cut(s) 618
BseMII CTCAG 3 cut(s) 111, 409, 486
BseNI ACTGG 1 cut(s) 622
BseRI GAGGAG 1 cut(s) 1157
BseXI GCAGC 3 cut(s) 792, 1081, 1243
Bsh1285I CGRYCG 1 cut(s) 1053
BshFI GGCC 4 cut(s) 549, 759, 906, 1057
BshNI GGYRCC 1 cut(s) 982
BshVI ATCGAT 1 cut(s) 423
BsiEI CGRYCG 1 cut(s) 1053
BsiHKAI GWGCWC 1 cut(s) 403
BsiSI CCGG 7 cut(s) 698, 756, 885, 903, 960, 986, 1181
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 618
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 4 cut(s) 549, 759, 906, 1057
Bsp119I TTCGAA 1 cut(s) 1265
Bsp1286I GDGCHC 2 cut(s) 403, 724
Bsp143I GATC 5 cut(s) 255, 424, 825, 1126, 1133
BspACI CCGC 2 cut(s) 601, 908
BspANI GGCC 4 cut(s) 549, 759, 906, 1057
BspCNI CTCAG 3 cut(s) 110, 408, 485
BspDI ATCGAT 1 cut(s) 423
BspHI TCATGA 1 cut(s) 237
BspLI GGNNCC 5 cut(s) 298, 622, 984, 1185, 1218
BspT104I TTCGAA 1 cut(s) 1265
BspT107I GGYRCC 1 cut(s) 982
BsrFI RCCGGY 2 cut(s) 755, 902
BsrI ACTGG 1 cut(s) 622
BssAI RCCGGY 2 cut(s) 755, 902
BssECI CCNNGG 2 cut(s) 288, 706
BssMI GATC 5 cut(s) 255, 424, 825, 1126, 1133
BssNI GRCGYC 1 cut(s) 657
BssSI CACGAG 1 cut(s) 717
BssT1I CCWWGG 1 cut(s) 706
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 3 cut(s) 329, 972, 1109
BstACI GRCGYC 1 cut(s) 657
BstBI TTCGAA 1 cut(s) 1265
BstC8I GCNNGC 1 cut(s) 908
BstDEI CTNAG 3 cut(s) 97, 395, 472
BstF5I GGATG 4 cut(s) 54, 597, 837, 932
BstH2I RGCGCY 2 cut(s) 357, 786
BstHHI GCGC 2 cut(s) 356, 785
BstKTI GATC 5 cut(s) 258, 427, 828, 1129, 1136
BstMBI GATC 5 cut(s) 255, 424, 825, 1126, 1133
BstMCI CGRYCG 1 cut(s) 1053
BstMWI GCNNNNNNNGC 2 cut(s) 719, 780
BstNI CCWGG 1 cut(s) 289
BstSCI CCNGG 3 cut(s) 287, 696, 1179
BstV1I GCAGC 3 cut(s) 792, 1081, 1243
Bsu15I ATCGAT 1 cut(s) 423
BsuRI GGCC 4 cut(s) 549, 759, 906, 1057
BsuTUI ATCGAT 1 cut(s) 423
BtrI CACGTC 1 cut(s) 1047
BtsCI GGATG 4 cut(s) 54, 597, 837, 932
BtsIMutI CAGTG 3 cut(s) 403, 615, 1143
Cac8I GCNNGC 1 cut(s) 908
CciI TCATGA 1 cut(s) 237
CfoI GCGC 2 cut(s) 356, 785
Cfr10I RCCGGY 2 cut(s) 755, 902
Cfr13I GGNCC 6 cut(s) 548, 621, 758, 905, 1055, 1183
ClaI ATCGAT 1 cut(s) 423
Csp6I GTAC 3 cut(s) 626, 806, 973
CspCI CAANNNNNGTGG 2 cut(s) 346, 381
CviAII CATG 4 cut(s) 238, 253, 452, 574
CviQI GTAC 3 cut(s) 626, 806, 973
DdeI CTNAG 3 cut(s) 97, 395, 472
DpnI GATC 5 cut(s) 257, 426, 827, 1128, 1135
DpnII GATC 5 cut(s) 255, 424, 825, 1126, 1133
Eco130I CCWWGG 1 cut(s) 706
Eco24I GRGCYC 1 cut(s) 724
Eco47I GGWCC 2 cut(s) 621, 1183
Eco47III AGCGCT 1 cut(s) 784
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 706
FaeI CATG 4 cut(s) 241, 256, 455, 577
FaqI GGGAC 1 cut(s) 140
FatI CATG 4 cut(s) 237, 252, 451, 573
FauI CCCGC 1 cut(s) 915
FauNDI CATATG 1 cut(s) 305
FbaI TGATCA 3 cut(s) 255, 825, 1126
FblI GTMKAC 1 cut(s) 1190
Fnu4HI GCNGC 3 cut(s) 781, 1070, 1257
FokI GGATG 4 cut(s) 41, 584, 824, 939
FriOI GRGCYC 1 cut(s) 724
Fsp4HI GCNGC 3 cut(s) 781, 1070, 1257
FspBI CTAG 6 cut(s) 215, 440, 653, 851, 1004, 1170
GlaI GCGC 2 cut(s) 355, 784
GluI GCNGC 3 cut(s) 781, 1070, 1257
GsuI CTGGAG 1 cut(s) 324
HaeII RGCGCY 2 cut(s) 357, 786
HaeIII GGCC 4 cut(s) 549, 759, 906, 1057
HapII CCGG 7 cut(s) 698, 756, 885, 903, 960, 986, 1181
HhaI GCGC 2 cut(s) 356, 785
Hin1I GRCGYC 1 cut(s) 657
Hin1II CATG 4 cut(s) 241, 256, 455, 577
Hin6I GCGC 2 cut(s) 354, 783
HinP1I GCGC 2 cut(s) 354, 783
HincII GTYRAC 1 cut(s) 1191
HindII GTYRAC 1 cut(s) 1191
HinfI GANTC 2 cut(s) 95, 750
HpaII CCGG 7 cut(s) 698, 756, 885, 903, 960, 986, 1181
HphI GGTGA 2 cut(s) 562, 745
Hpy166II GTNNAC 3 cut(s) 226, 1191, 1202
Hpy188I TCNGA 5 cut(s) 100, 195, 265, 301, 1249
Hpy188III TCNNGA 6 cut(s) 238, 341, 410, 434, 1091, 1162
Hpy8I GTNNAC 3 cut(s) 226, 1191, 1202
Hpy99I CGWCG 3 cut(s) 1051, 1192, 1195
HpyAV CCTTC 5 cut(s) 55, 143, 170, 622, 1225
HpyCH4III ACNGT 3 cut(s) 329, 972, 1109
HpyCH4IV ACGT 3 cut(s) 657, 1046, 1193
HpyCH4V TGCA 5 cut(s) 90, 309, 497, 837, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 719, 780
HpyF3I CTNAG 3 cut(s) 97, 395, 472
HpySE526I ACGT 3 cut(s) 657, 1046, 1193
Hsp92I GRCGYC 1 cut(s) 657
Hsp92II CATG 4 cut(s) 241, 256, 455, 577
HspAI GCGC 2 cut(s) 354, 783
Ksp22I TGATCA 3 cut(s) 255, 825, 1126
Kzo9I GATC 5 cut(s) 255, 424, 825, 1126, 1133
LmnI GCTCC 3 cut(s) 302, 406, 710
Lsp1109I GCAGC 3 cut(s) 792, 1081, 1243
LweI GCATC 7 cut(s) 285, 783, 846, 856, 871, 917, 1126
MaeI CTAG 6 cut(s) 215, 440, 653, 851, 1004, 1170
MaeII ACGT 3 cut(s) 657, 1046, 1193
MaeIII GTNAC 1 cut(s) 168
MalI GATC 5 cut(s) 257, 426, 827, 1128, 1135
MboI GATC 5 cut(s) 255, 424, 825, 1126, 1133
MboII GAAGA 2 cut(s) 40, 1114
MhlI GDGCHC 2 cut(s) 403, 724
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 324, 1056
MseI TTAA 5 cut(s) 125, 179, 669, 860, 951
MslI CAYNNNNRTG 1 cut(s) 925
MspA1I CMGCKG 1 cut(s) 1072
MspI CCGG 7 cut(s) 698, 756, 885, 903, 960, 986, 1181
MspR9I CCNGG 3 cut(s) 289, 698, 1181
MvaI CCWGG 1 cut(s) 289
MwoI GCNNNNNNNGC 2 cut(s) 719, 780
NciI CCSGG 2 cut(s) 698, 1181
NdeI CATATG 1 cut(s) 305
NdeII GATC 5 cut(s) 255, 424, 825, 1126, 1133
NlaIII CATG 4 cut(s) 241, 256, 455, 577
NlaIV GGNNCC 5 cut(s) 298, 622, 984, 1185, 1218
NspV TTCGAA 1 cut(s) 1265
PagI TCATGA 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 420
PfeI GAWTC 1 cut(s) 750
PfoI TCCNGGA 1 cut(s) 696
PkrI GCNGC 3 cut(s) 782, 1071, 1258
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PshBI ATTAAT 2 cut(s) 179, 669
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 5 cut(s) 298, 622, 984, 1185, 1218
PspPI GGNCC 6 cut(s) 548, 621, 758, 905, 1055, 1183
PvuII CAGCTG 1 cut(s) 1072
RsaI GTAC 3 cut(s) 627, 807, 974
RsaNI GTAC 3 cut(s) 626, 806, 973
RseI CAYNNNNRTG 1 cut(s) 925
SalI GTCGAC 1 cut(s) 1189
SaqAI TTAA 5 cut(s) 125, 179, 669, 860, 951
SatI GCNGC 3 cut(s) 781, 1070, 1257
Sau3AI GATC 5 cut(s) 255, 424, 825, 1126, 1133
Sau96I GGNCC 6 cut(s) 548, 621, 758, 905, 1055, 1183
ScaI AGTACT 1 cut(s) 807
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 3 cut(s) 289, 698, 1181
SduI GDGCHC 2 cut(s) 403, 724
SfaNI GCATC 7 cut(s) 285, 783, 846, 856, 871, 917, 1126
SfuI TTCGAA 1 cut(s) 1265
SgrDI CGTCGACG 1 cut(s) 1189
SinI GGWCC 2 cut(s) 621, 1183
SmiMI CAYNNNNRTG 1 cut(s) 925
SmlI CTYRAG 1 cut(s) 1096
SmoI CTYRAG 1 cut(s) 1096
SsiI CCGC 2 cut(s) 601, 908
SspMI CTAG 6 cut(s) 215, 440, 653, 851, 1004, 1170
StyD4I CCNGG 3 cut(s) 287, 696, 1179
StyI CCWWGG 1 cut(s) 706
TaaI ACNGT 3 cut(s) 329, 972, 1109
TaiI ACGT 3 cut(s) 660, 1049, 1196
TaqI TCGA 4 cut(s) 423, 1190, 1229, 1265
TaqII GACCGA 1 cut(s) 1039
TatI WGTACW 1 cut(s) 805
TfiI GAWTC 1 cut(s) 750
Tru1I TTAA 5 cut(s) 125, 179, 669, 860, 951
Tru9I TTAA 5 cut(s) 125, 179, 669, 860, 951
TscAI CASTG 3 cut(s) 403, 622, 1143
TseI GCWGC 3 cut(s) 780, 1069, 1256
TspDTI ATGAA 9 cut(s) 40, 409, 440, 590, 655, 673, 777, 861, 909
TspGWI ACGGA 2 cut(s) 613, 990
TspRI CASTG 3 cut(s) 403, 622, 1143
VpaK11BI GGWCC 2 cut(s) 621, 1183
VspI ATTAAT 2 cut(s) 179, 669
XapI RAATTY 3 cut(s) 7, 636, 1009
XmiI GTMKAC 1 cut(s) 1190
XspI CTAG 6 cut(s) 215, 440, 653, 851, 1004, 1170
ZraI GACGTC 1 cut(s) 658
ZrmI AGTACT 1 cut(s) 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.