Rh6AG080000

At4g06744-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0016747
Physical Location & Seq
Forward (+)
11633361 .. 11635492
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG080000.1

Sequence Viewer

Length: 1218 bp
ATGGGAAGCTTCTGTTTACCATTTGTGCGTCTACTTTATACCCTCGTCATCATCCTTCCGTGTTCCTTCAATACTGTAATTTGTACAGACCATGAGACTCCATCACTATTACCTCCATTAATCCCTGAACTCTTGAACTTCTCAGACCAAAGACTATCATTGATTTACCCCATTATACAATCCTTCAAGAACATCATCACGTCAGATCCATTAGGCATCACCAAAACCTGGGTTGGCGCTGACATATGCAAATACAAGGGCTTCTACTGTGACAAACCACCGGACAATGTCACCGCCACTGCACTCGCCTCCATAGATTTCAACGGCTTCCAACTCAGTGCTCCAACTCTTGACGGCTTCATAGATCAACTTCCTGACCTTGCACTCTTTCATGCTAACTCCAACAAATTCTCAGGTATCATTTCACCAAAAATTGCAAATCTCAAATACCTCTATGAGCTTGACATAAGCAACAACAACTTCTTTGGCCCTTTTCCCTTGGCAGTACTCACCATGAATAGCCTCTCATTCTTGGACATCCGGTACAACTCTTTTACCGGATCCGTGCCACCCGAAATTTTCGAGCAAACCCTGGATGTTCTCTTCCTCAACAACAACAATTTCATACAAAAACTCCCTGTTAATCTTGGTGCTACACCAGCGCTTTATCTCACCTTAGCCAACAACAAGTTCATCGGCCCCATCCCAAAAAGCATCGGAAGTGCTTCATCAACTTTGGTAGAAGCGCTGCTCTTGAACAATTTGTTAACCGGTTGCATCCCTTATGAACTAGGGTTTTTGAGAGATGCCACTGTAATTGATGCCGGAAACAACCTGTTGACCGGCCCTTTGCCGTGTTCATTGGGATGCTTGGACAAGATAGAACAGCTCAACTTTGCCGAGAACTTTCTCTATGGACGAGTGCCGGAGGTGCTTTGTGAACTAGGGAATTTGGCCAATCTATCTCTGTCAAACAACTACTTTACTGGAGCAGGGATAGCATGTAGAAAGCTGATAAAAAAGGGTGTCCTTGACATAAGAAACAATTGTATTCATGATCTTGCAGATCAAAGATCAGTGGAGGAATGTGCCTTGTTCTTCTTGACCCCTAAAAGCTGCCAGCGCCCTGAGACATTTAGTTCAATTCCTTGTAAGGCTCCACCAAAATCAAAGAGGAAATCGGCTCCATATTCTGCAGCTCCGAAGCAGAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

405

Amino Acids

44.54

Weight (kDa)

6.33

Isoelectric Point (pI)

30.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016747)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05540
malus_domestica MD05G1086600.v1.1
prunus_persica Prupe.8G131300_v2.0.a1
pyrus_communis pycom05g08600
rosa_chinensis RchiOBHm_Chr6g0255851
rosa_laevigata RLG00000014811
rosa_multiflora Rmu_sc0001163.1_g000035
rosa_roxburghii Rroxscaffold_7G00209590
rosa_rugosa Rorug05G0561700
rosa_samantha Rh6AG080000 Rh6BG072900 Rh6CG070900 Rh6DG068200
rosa_wichuraiana Rw6G006900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 228
AccI GTMKAC 1 cut(s) 31
AciI CCGC 1 cut(s) 294
AclWI GGATC 3 cut(s) 200, 555, 568
AcoI YGGCCR 1 cut(s) 954
AcsI RAATTY 3 cut(s) 407, 576, 949
AfaI GTAC 3 cut(s) 85, 507, 545
AfeI AGCGCT 2 cut(s) 663, 747
AfiI CCNNNNNNNGG 1 cut(s) 228
AgeI ACCGGT 1 cut(s) 770
AgsI TTSAA 6 cut(s) 70, 136, 187, 322, 757, 1143
AjiI CACGTC 1 cut(s) 201
AjnI CCWGG 2 cut(s) 227, 591
AjuI GAANNNNNNNTTGG 2 cut(s) 674, 706
AluBI AGCT 6 cut(s) 9, 460, 889, 1012, 1116, 1199
AluI AGCT 6 cut(s) 9, 460, 889, 1012, 1116, 1199
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 2 cut(s) 89, 1124
AlwI GGATC 3 cut(s) 200, 555, 568
Aor51HI AGCGCT 2 cut(s) 663, 747
AoxI GGCC 4 cut(s) 487, 697, 844, 954
ApeKI GCWGC 3 cut(s) 748, 1116, 1196
ApoI RAATTY 3 cut(s) 407, 576, 949
ArsI GACNNNNNNTTYG 2 cut(s) 832, 864
AseI ATTAAT 1 cut(s) 119
AsiGI ACCGGT 1 cut(s) 770
Asp700I GAANNNNTTC 1 cut(s) 724
AspLEI GCGC 4 cut(s) 239, 664, 748, 1125
AspS9I GGNCC 3 cut(s) 488, 698, 845
AsuHPI GGTGA 5 cut(s) 211, 283, 417, 502, 664
BalI TGGCCA 1 cut(s) 956
BamHI GGATCC 1 cut(s) 560
Bbv12I GWGCWC 1 cut(s) 343
BbvI GCAGC 3 cut(s) 735, 1103, 1208
BccI CCATC 2 cut(s) 109, 710
BceAI ACGGC 3 cut(s) 340, 370, 838
BciT130I CCWGG 2 cut(s) 229, 593
BcoDI GTCTC 2 cut(s) 89, 1124
BfaI CTAG 2 cut(s) 791, 944
BfmI CTRYAG 1 cut(s) 1194
BfoI RGCGCY 4 cut(s) 240, 665, 749, 1126
BisI GCNGC 3 cut(s) 749, 1117, 1197
BlsI GCNGC 3 cut(s) 750, 1118, 1198
BmcAI AGTACT 1 cut(s) 507
Bme1390I CCNGG 2 cut(s) 229, 593
BmgBI CACGTC 1 cut(s) 201
BmgT120I GGNCC 3 cut(s) 488, 698, 845
BmiI GGNNCC 4 cut(s) 562, 700, 1158, 1185
BmrFI CCNGG 2 cut(s) 229, 593
BmsI GCATC 6 cut(s) 225, 723, 786, 796, 811, 857
BpmI CTGGAG 1 cut(s) 1008
Bpu10I CCTNAGC 1 cut(s) 676
BsaJI CCNNGG 3 cut(s) 228, 498, 591
BsaWI WCCGGW 4 cut(s) 280, 540, 557, 770
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse118I RCCGGY 2 cut(s) 770, 842
Bse1I ACTGG 1 cut(s) 991
BseBI CCWGG 2 cut(s) 229, 593
BseDI CCNNGG 3 cut(s) 228, 498, 591
BseGI GGATG 6 cut(s) 51, 537, 601, 702, 777, 872
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMII CTCAG 4 cut(s) 156, 349, 426, 1119
BseNI ACTGG 1 cut(s) 991
BseXI GCAGC 3 cut(s) 735, 1103, 1208
BsgI GTGCAG 1 cut(s) 285
BshFI GGCC 4 cut(s) 489, 699, 846, 956
BshTI ACCGGT 1 cut(s) 770
BsiHKAI GWGCWC 1 cut(s) 343
BsiSI CCGG 7 cut(s) 281, 541, 558, 771, 825, 843, 926
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 2 cut(s) 89, 1124
BsnI GGCC 4 cut(s) 489, 699, 846, 956
Bsp1286I GDGCHC 1 cut(s) 343
Bsp1407I TGTACA 1 cut(s) 83
Bsp143I GATC 6 cut(s) 205, 364, 560, 1057, 1066, 1073
BspACI CCGC 1 cut(s) 294
BspANI GGCC 4 cut(s) 489, 699, 846, 956
BspCNI CTCAG 4 cut(s) 155, 348, 425, 1120
BspHI TCATGA 1 cut(s) 1054
BspLI GGNNCC 4 cut(s) 562, 700, 1158, 1185
BspMAI CTGCAG 1 cut(s) 1198
BspPI GGATC 3 cut(s) 200, 555, 568
BsrFI RCCGGY 2 cut(s) 770, 842
BsrGI TGTACA 1 cut(s) 83
BsrI ACTGG 1 cut(s) 991
BssAI RCCGGY 2 cut(s) 770, 842
BssECI CCNNGG 3 cut(s) 228, 498, 591
BssMI GATC 6 cut(s) 205, 364, 560, 1057, 1066, 1073
BssT1I CCWWGG 1 cut(s) 498
Bst2UI CCWGG 2 cut(s) 229, 593
Bst4CI ACNGT 3 cut(s) 76, 269, 814
Bst6I CTCTTC 1 cut(s) 608
BstAUI TGTACA 1 cut(s) 83
BstC8I GCNNGC 1 cut(s) 1121
BstDEI CTNAG 5 cut(s) 142, 335, 412, 676, 1128
BstF5I GGATG 6 cut(s) 51, 537, 601, 702, 777, 872
BstH2I RGCGCY 4 cut(s) 240, 665, 749, 1126
BstHHI GCGC 4 cut(s) 239, 664, 748, 1125
BstKTI GATC 6 cut(s) 208, 367, 563, 1060, 1069, 1076
BstMAI GTCTC 2 cut(s) 89, 1124
BstMBI GATC 6 cut(s) 205, 364, 560, 1057, 1066, 1073
BstMWI GCNNNNNNNGC 4 cut(s) 659, 931, 998, 1122
BstNI CCWGG 2 cut(s) 229, 593
BstNSI RCATGY 1 cut(s) 1005
BstSCI CCNGG 2 cut(s) 227, 591
BstSFI CTRYAG 1 cut(s) 1194
BstV1I GCAGC 3 cut(s) 735, 1103, 1208
BstX2I RGATCY 2 cut(s) 205, 560
BstYI RGATCY 2 cut(s) 205, 560
BsuRI GGCC 4 cut(s) 489, 699, 846, 956
BtrI CACGTC 1 cut(s) 201
BtsCI GGATG 6 cut(s) 51, 537, 601, 702, 777, 872
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 4 cut(s) 297, 343, 810, 1083
Cac8I GCNNGC 1 cut(s) 1121
CciI TCATGA 1 cut(s) 1054
CfoI GCGC 4 cut(s) 239, 664, 748, 1125
Cfr10I RCCGGY 2 cut(s) 770, 842
Cfr13I GGNCC 3 cut(s) 488, 698, 845
CseI GACGC 1 cut(s) 17
Csp6I GTAC 3 cut(s) 84, 506, 544
CspAI ACCGGT 1 cut(s) 770
CspCI CAANNNNNGTGG 2 cut(s) 799, 834
CviAII CATG 5 cut(s) 92, 392, 514, 1002, 1055
CviQI GTAC 3 cut(s) 84, 506, 544
DdeI CTNAG 5 cut(s) 142, 335, 412, 676, 1128
DpnI GATC 6 cut(s) 207, 366, 562, 1059, 1068, 1075
DpnII GATC 6 cut(s) 205, 364, 560, 1057, 1066, 1073
EaeI YGGCCR 1 cut(s) 954
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
Eco130I CCWWGG 1 cut(s) 498
Eco47III AGCGCT 2 cut(s) 663, 747
EcoRII CCWGG 2 cut(s) 227, 591
EcoT14I CCWWGG 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 5 cut(s) 95, 395, 517, 1005, 1058
FatI CATG 5 cut(s) 91, 391, 513, 1001, 1054
FauNDI CATATG 1 cut(s) 245
FblI GTMKAC 1 cut(s) 31
Fnu4HI GCNGC 3 cut(s) 749, 1117, 1197
FokI GGATG 6 cut(s) 38, 524, 608, 689, 764, 879
Fsp4HI GCNGC 3 cut(s) 749, 1117, 1197
FspBI CTAG 2 cut(s) 791, 944
GlaI GCGC 4 cut(s) 238, 663, 747, 1124
GluI GCNGC 3 cut(s) 749, 1117, 1197
GsuI CTGGAG 1 cut(s) 1008
HaeII RGCGCY 4 cut(s) 240, 665, 749, 1126
HaeIII GGCC 4 cut(s) 489, 699, 846, 956
HapII CCGG 7 cut(s) 281, 541, 558, 771, 825, 843, 926
HgaI GACGC 1 cut(s) 17
HhaI GCGC 4 cut(s) 239, 664, 748, 1125
Hin1II CATG 5 cut(s) 95, 395, 517, 1005, 1058
Hin6I GCGC 4 cut(s) 237, 662, 746, 1123
HinP1I GCGC 4 cut(s) 237, 662, 746, 1123
HincII GTYRAC 2 cut(s) 768, 840
HindII GTYRAC 2 cut(s) 768, 840
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 2 cut(s) 97, 1212
HpaI GTTAAC 1 cut(s) 768
HpaII CCGG 7 cut(s) 281, 541, 558, 771, 825, 843, 926
HphI GGTGA 5 cut(s) 211, 283, 417, 502, 664
Hpy166II GTNNAC 5 cut(s) 17, 32, 768, 840, 941
Hpy188I TCNGA 5 cut(s) 145, 205, 719, 1203, 1217
Hpy188III TCNNGA 7 cut(s) 133, 187, 350, 374, 754, 1055, 1102
Hpy8I GTNNAC 5 cut(s) 17, 32, 768, 840, 941
HpyAV CCTTC 3 cut(s) 65, 76, 193
HpyCH4III ACNGT 3 cut(s) 76, 269, 814
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 7 cut(s) 249, 302, 383, 437, 777, 1064, 1196
HpyF10VI GCNNNNNNNGC 4 cut(s) 659, 931, 998, 1122
HpyF3I CTNAG 5 cut(s) 142, 335, 412, 676, 1128
HpySE526I ACGT 1 cut(s) 200
Hsp92II CATG 5 cut(s) 95, 395, 517, 1005, 1058
HspAI GCGC 4 cut(s) 237, 662, 746, 1123
KspAI GTTAAC 1 cut(s) 768
Kzo9I GATC 6 cut(s) 205, 364, 560, 1057, 1066, 1073
LmnI GCTCC 5 cut(s) 346, 989, 1162, 1189, 1204
Lsp1109I GCAGC 3 cut(s) 735, 1103, 1208
LweI GCATC 6 cut(s) 225, 723, 786, 796, 811, 857
MaeI CTAG 2 cut(s) 791, 944
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 269, 289
MalI GATC 6 cut(s) 207, 366, 562, 1059, 1068, 1075
MboI GATC 6 cut(s) 205, 364, 560, 1057, 1066, 1073
MboII GAAGA 2 cut(s) 595, 1090
MfeI CAATTG 1 cut(s) 1045
MflI RGATCY 2 cut(s) 205, 560
MhlI GDGCHC 1 cut(s) 343
MlsI TGGCCA 1 cut(s) 956
MluNI TGGCCA 1 cut(s) 956
MlyI GAGTC 1 cut(s) 91
MmeI TCCRAC 3 cut(s) 355, 368, 426
MnlI CCTC 9 cut(s) 53, 123, 319, 461, 533, 617, 922, 1075, 1167
Mox20I TGGCCA 1 cut(s) 956
MroXI GAANNNNTTC 1 cut(s) 724
MscI TGGCCA 1 cut(s) 956
MseI TTAA 3 cut(s) 119, 642, 767
MslI CAYNNNNRTG 1 cut(s) 865
Msp20I TGGCCA 1 cut(s) 956
MspI CCGG 7 cut(s) 281, 541, 558, 771, 825, 843, 926
MspR9I CCNGG 2 cut(s) 229, 593
MunI CAATTG 1 cut(s) 1045
MvaI CCWGG 2 cut(s) 229, 593
MwoI GCNNNNNNNGC 4 cut(s) 659, 931, 998, 1122
NdeI CATATG 1 cut(s) 245
NdeII GATC 6 cut(s) 205, 364, 560, 1057, 1066, 1073
NlaIII CATG 5 cut(s) 95, 395, 517, 1005, 1058
NlaIV GGNNCC 4 cut(s) 562, 700, 1158, 1185
NmeAIII GCCGAG 1 cut(s) 925
NmuCI GTSAC 2 cut(s) 269, 289
NspI RCATGY 1 cut(s) 1005
PagI TCATGA 1 cut(s) 1054
PdmI GAANNNNTTC 1 cut(s) 724
PfeI GAWTC 1 cut(s) 1212
PflFI GACNNNGTC 1 cut(s) 287
PflMI CCANNNNNTGG 1 cut(s) 228
PinAI ACCGGT 1 cut(s) 770
PkrI GCNGC 3 cut(s) 750, 1118, 1198
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
PshBI ATTAAT 1 cut(s) 119
Psp6I CCWGG 2 cut(s) 227, 591
PspGI CCWGG 2 cut(s) 227, 591
PspN4I GGNNCC 4 cut(s) 562, 700, 1158, 1185
PspPI GGNCC 3 cut(s) 488, 698, 845
PstI CTGCAG 1 cut(s) 1198
PsuI RGATCY 2 cut(s) 205, 560
PsyI GACNNNGTC 1 cut(s) 287
RsaI GTAC 3 cut(s) 85, 507, 545
RsaNI GTAC 3 cut(s) 84, 506, 544
RseI CAYNNNNRTG 1 cut(s) 865
SaqAI TTAA 3 cut(s) 119, 642, 767
SatI GCNGC 3 cut(s) 749, 1117, 1197
Sau3AI GATC 6 cut(s) 205, 364, 560, 1057, 1066, 1073
Sau96I GGNCC 3 cut(s) 488, 698, 845
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 1 cut(s) 91
ScrFI CCNGG 2 cut(s) 229, 593
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 6 cut(s) 225, 723, 786, 796, 811, 857
SfcI CTRYAG 1 cut(s) 1194
SmiMI CAYNNNNRTG 1 cut(s) 865
SsiI CCGC 1 cut(s) 294
SspMI CTAG 2 cut(s) 791, 944
StyD4I CCNGG 2 cut(s) 227, 591
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 3 cut(s) 76, 269, 814
TaiI ACGT 1 cut(s) 203
TaqI TCGA 1 cut(s) 582
TatI WGTACW 2 cut(s) 83, 505
TfiI GAWTC 1 cut(s) 1212
Tru1I TTAA 3 cut(s) 119, 642, 767
Tru9I TTAA 3 cut(s) 119, 642, 767
TscAI CASTG 4 cut(s) 304, 343, 817, 1083
TseFI GTSAC 2 cut(s) 269, 289
TseI GCWGC 3 cut(s) 748, 1116, 1196
Tsp45I GTSAC 2 cut(s) 269, 289
TspDTI ATGAA 9 cut(s) 349, 380, 530, 613, 682, 717, 801, 849, 1043
TspGWI ACGGA 2 cut(s) 48, 553
TspRI CASTG 4 cut(s) 304, 343, 817, 1083
Tth111I GACNNNGTC 1 cut(s) 287
Van91I CCANNNNNTGG 1 cut(s) 228
VspI ATTAAT 1 cut(s) 119
XapI RAATTY 3 cut(s) 407, 576, 949
XceI RCATGY 1 cut(s) 1005
XmiI GTMKAC 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 724
XspI CTAG 2 cut(s) 791, 944
ZrmI AGTACT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.