FvH4_2g06230

Protein of unknown function (DUF565)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012174
Physical Location & Seq
Forward (+)
5174409 .. 5176390
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06230.t1

Sequence Viewer

Length: 468 bp
ATGGCTTGTTCGTTTAGTTTGCCATCAACTGGCCTCCAAACATTTGGATTGAATCCTCAAAGCAGAGTTTCACAAGCGAAATATGGAGTTTTTACTTTGCGATCCTCACCTGGGTCTCTTCGTGTCCGGGCAGTGCAAGAGACTGGAGGGTCCCGCAGACTAGTTGATATAATCAGAACTGTGCCAGAGCTCTCAAGTAACTACTTTCGAAGTCCATCTCGTAGGGCTCTCTTTGGGGGAATATCTTTGTTGGGTGGGTTTTATGTTGCACAAACGATATCTCTGTCATTTGGAGCTTTAGGAGTCAATGATGTTATTGCTGCAGTTGTGTGTGTTCTCCTGACAGAATATGTTACCAAATTTTATTACAGCAGACCAAAAGTAACATTTCCTATTGCTCTTCTCAACAATTTCAAAATGGGATTCACTTATGGCCTGTTCATAGATGCTTTTAAGCTTGCCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

156

Amino Acids

16.9

Weight (kDa)

10.14

Isoelectric Point (pI)

61.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 101 - 155 1.7e-19 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43050
fragaria_vesca FvH4_2g06230
malus_domestica MD05G1079400.v1.1
prunus_persica Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1
pyrus_communis pycom05g07250
rosa_chinensis RchiOBHm_Chr6g0256951
rosa_laevigata RLG00000014664
rosa_multiflora Rmu_co8075354.1_g000001 Rmu_sc0000659.1_g000043
rosa_roxburghii Rroxscaffold_7G00208360
rosa_rugosa Rorug05G0576200
rosa_samantha Rh6AG094800 Rh6BG086000 Rh6CG082100 Rh6DG077800
rosa_wichuraiana Rw6G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 148
AccB7I CCANNNNNTGG 1 cut(s) 29
AciI CCGC 1 cut(s) 154
AclWI GGATC 1 cut(s) 96
AcsI RAATTY 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 29, 111
AgsI TTSAA 2 cut(s) 52, 415
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 3 cut(s) 190, 296, 457
AluI AGCT 3 cut(s) 190, 296, 457
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 120, 134
AlwI GGATC 1 cut(s) 96
AoxI GGCC 2 cut(s) 31, 433
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 150
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 99
AsuII TTCGAA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 150
BanII GRGCYC 2 cut(s) 192, 229
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 307
BccI CCATC 2 cut(s) 31, 223
BciT130I CCWGG 1 cut(s) 111
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 120, 134
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 321
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 2 cut(s) 111, 128
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 1 cut(s) 150
BmiI GGNNCC 2 cut(s) 151, 152
BmrFI CCNGG 2 cut(s) 111, 128
BmsI GCATC 1 cut(s) 436
BpmI CTGGAG 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 110
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 111
Bse1I ACTGG 3 cut(s) 34, 148, 462
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 1 cut(s) 110
BseLI CCNNNNNNNGG 2 cut(s) 29, 111
BseNI ACTGG 3 cut(s) 34, 148, 462
BseXI GCAGC 1 cut(s) 307
BshFI GGCC 2 cut(s) 33, 435
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 1 cut(s) 127
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 2 cut(s) 29, 111
BsmAI GTCTC 2 cut(s) 120, 134
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 2 cut(s) 33, 435
Bso31I GGTCTC 1 cut(s) 120
Bsp119I TTCGAA 1 cut(s) 208
Bsp1286I GDGCHC 2 cut(s) 192, 229
Bsp143I GATC 1 cut(s) 101
BspACI CCGC 1 cut(s) 154
BspANI GGCC 2 cut(s) 33, 435
BspLI GGNNCC 2 cut(s) 151, 152
BspMAI CTGCAG 1 cut(s) 325
BspPI GGATC 1 cut(s) 96
BspQI GCTCTTC 1 cut(s) 405
BspT104I TTCGAA 1 cut(s) 208
BspTNI GGTCTC 1 cut(s) 120
BsrI ACTGG 3 cut(s) 34, 148, 462
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 1 cut(s) 101
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 123, 405
BstBI TTCGAA 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 459
BstKTI GATC 1 cut(s) 104
BstMAI GTCTC 2 cut(s) 120, 134
BstMBI GATC 1 cut(s) 101
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 2 cut(s) 109, 126
BstSFI CTRYAG 1 cut(s) 321
BstV1I GCAGC 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 44
BsuRI GGCC 2 cut(s) 33, 435
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 150
CviJI RGCY 7 cut(s) 5, 33, 190, 227, 296, 435, 457
CviKI_1 RGCY 7 cut(s) 5, 33, 190, 227, 296, 435, 457
DpnI GATC 1 cut(s) 103
DpnII GATC 1 cut(s) 101
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
Eam1104I CTCTTC 2 cut(s) 123, 405
EarI CTCTTC 2 cut(s) 123, 405
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 2 cut(s) 192, 229
Eco31I GGTCTC 1 cut(s) 120
Eco32I GATATC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 150
Eco53kI GAGCTC 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 109
EcoRV GATATC 1 cut(s) 279
EcoT38I GRGCYC 2 cut(s) 192, 229
FaiI YATR 6 cut(s) 84, 170, 264, 351, 432, 443
FaqI GGGAC 1 cut(s) 136
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 1 cut(s) 321
FriOI GRGCYC 2 cut(s) 192, 229
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 1 cut(s) 161
GluI GCNGC 1 cut(s) 321
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 2 cut(s) 33, 435
HapII CCGG 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 3 cut(s) 52, 303, 423
HpaII CCGG 1 cut(s) 127
HphI GGTGA 1 cut(s) 99
Hpy188I TCNGA 1 cut(s) 176
Hpy188III TCNNGA 1 cut(s) 340
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 3 cut(s) 136, 269, 323
KflI GGGWCCC 1 cut(s) 150
Kzo9I GATC 1 cut(s) 101
LguI GCTCTTC 1 cut(s) 405
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 8 cut(s) 15, 96, 123, 129, 140, 198, 353, 449
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 161
MaeIII GTNAC 3 cut(s) 197, 352, 382
MalI GATC 1 cut(s) 103
MboI GATC 1 cut(s) 101
MboII GAAGA 2 cut(s) 110, 392
MhlI GDGCHC 2 cut(s) 192, 229
MluCI AATT 2 cut(s) 359, 409
MlyI GAGTC 1 cut(s) 312
MnlI CCTC 4 cut(s) 44, 66, 115, 140
MseI TTAA 2 cut(s) 453, 466
MspI CCGG 1 cut(s) 127
MspR9I CCNGG 2 cut(s) 111, 128
MvaI CCWGG 1 cut(s) 111
NciI CCSGG 1 cut(s) 128
NdeII GATC 1 cut(s) 101
NlaIV GGNNCC 2 cut(s) 151, 152
NspV TTCGAA 1 cut(s) 208
PciSI GCTCTTC 1 cut(s) 405
PfeI GAWTC 2 cut(s) 52, 423
PflMI CCANNNNNTGG 1 cut(s) 29
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
PpuMI RGGWCCY 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 2 cut(s) 151, 152
PspPI GGNCC 1 cut(s) 150
PspPPI RGGWCCY 1 cut(s) 150
PstI CTGCAG 1 cut(s) 325
SacI GAGCTC 1 cut(s) 192
SapI GCTCTTC 1 cut(s) 405
SaqAI TTAA 2 cut(s) 453, 466
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 1 cut(s) 101
Sau96I GGNCC 1 cut(s) 150
SchI GAGTC 1 cut(s) 312
ScrFI CCNGG 2 cut(s) 111, 128
SduI GDGCHC 2 cut(s) 192, 229
SetI ASST 4 cut(s) 112, 192, 298, 459
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 208
SinI GGWCC 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
SpeI ACTAGT 1 cut(s) 160
Sse9I AATT 2 cut(s) 359, 409
SsiI CCGC 1 cut(s) 154
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 2 cut(s) 109, 126
TaaI ACNGT 1 cut(s) 181
TaqI TCGA 1 cut(s) 208
TasI AATT 2 cut(s) 359, 409
TfiI GAWTC 2 cut(s) 52, 423
Tru1I TTAA 2 cut(s) 453, 466
Tru9I TTAA 2 cut(s) 453, 466
TscAI CASTG 1 cut(s) 138
TseI GCWGC 1 cut(s) 320
TspDTI ATGAA 1 cut(s) 430
TspRI CASTG 1 cut(s) 138
Van91I CCANNNNNTGG 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 150
XapI RAATTY 1 cut(s) 359
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.