RLG00000014664

Protein of unknown function (DUF565)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012174
Physical Location & Seq
Reverse (-)
55952055 .. 55954219
2165 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014664

Sequence Viewer

Length: 639 bp
ATGAGAACCCTAAAAGAATTAACCTCGTCCACTGGGCAATGCATTGTCATAAGAAAATCCAGAGAAAAATTTACAATTGAGGCAGAGGTCTCCATTGTCCACAGAGACAAACAAGAATCTGAATTGGACGTAGGCTTTGAGCGACCAGCGGTTCATTTAGAGCTCCCTCAGCCTTTCCATTTCGTTGAGGTTGAAAAACGAGGCCTCCATAAATTTGGATTGAGTCCTCAAAGCAGAGTTTCGCACATGAAATATGGAGTTTTCACTTTGAGATCCTCACCTGGGTTTCTTCGTGTCCGGGCTGTGCAAGAGAATGGAGGGTCCCGCAGACTAGTTGATATAATCAGAACCGTGCCAGAGCTCTCGAGGAACTACTTTCGAAGTCCATCTCGTAGGGCTCTCTTTGGGGGAATATCTTTGTTGGGTGGGTTTTATGTTGCCCAAACAATATCTCTGTCATTTGGAGCTTTAGGAGTCAATGATGTTATTGCTGCAGTTGTGTGTGTTCTCCTGACAGAATATGTAACCAAATTTTATTACAGCAGACCAAAAGTAACATTCCCCGTTGCTCTTCTCAACAATTTCAAAATGGGATTCACTTATGGTCTGTTCATAGATGCTTTTAAGCTTGCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

213

Amino Acids

23.9

Weight (kDa)

9.91

Isoelectric Point (pI)

55.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 158 - 212 2.8e-19 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43050
fragaria_vesca FvH4_2g06230
malus_domestica MD05G1079400.v1.1
prunus_persica Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1
pyrus_communis pycom05g07250
rosa_chinensis RchiOBHm_Chr6g0256951
rosa_laevigata RLG00000014664
rosa_multiflora Rmu_co8075354.1_g000001 Rmu_sc0000659.1_g000043
rosa_roxburghii Rroxscaffold_7G00208360
rosa_rugosa Rorug05G0576200
rosa_samantha Rh6AG094800 Rh6BG086000 Rh6CG082100 Rh6DG077800
rosa_wichuraiana Rw6G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 149, 325
AclWI GGATC 1 cut(s) 267
AcsI RAATTY 3 cut(s) 68, 212, 530
AfiI CCNNNNNNNGG 1 cut(s) 282
AgsI TTSAA 2 cut(s) 194, 586
AhlI ACTAGT 1 cut(s) 331
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 4 cut(s) 163, 361, 467, 628
AluI AGCT 4 cut(s) 163, 361, 467, 628
Alw21I GWGCWC 2 cut(s) 165, 363
Alw26I GTCTC 2 cut(s) 94, 99
AlwI GGATC 1 cut(s) 267
Ama87I CYCGRG 1 cut(s) 364
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 3 cut(s) 68, 212, 530
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 270
AsuII TTCGAA 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 364
AvaII GGWCC 1 cut(s) 321
BanII GRGCYC 3 cut(s) 165, 363, 400
Bbv12I GWGCWC 2 cut(s) 165, 363
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 478
BccI CCATC 1 cut(s) 394
BciT130I CCWGG 1 cut(s) 282
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 94, 99
BcuI ACTAGT 1 cut(s) 331
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 492
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 2 cut(s) 282, 299
Bme18I GGWCC 1 cut(s) 321
BmeT110I CYCGRG 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 2 cut(s) 322, 323
BmrFI CCNGG 2 cut(s) 282, 299
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 607
BmuI ACTGGG 1 cut(s) 42
Bpu10I CCTNAGC 1 cut(s) 168
Bpu14I TTCGAA 1 cut(s) 379
BpuMI CCSGG 1 cut(s) 299
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 1 cut(s) 281
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse1I ACTGG 2 cut(s) 37, 633
Bse3DI GCAATG 1 cut(s) 44
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 281
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMI GCAATG 1 cut(s) 44
BseMII CTCAG 1 cut(s) 182
BseNI ACTGG 2 cut(s) 37, 633
BseXI GCAGC 1 cut(s) 478
BshFI GGCC 1 cut(s) 204
BsiHKAI GWGCWC 2 cut(s) 165, 363
BsiHKCI CYCGRG 1 cut(s) 364
BsiSI CCGG 1 cut(s) 298
BslFI GGGAC 1 cut(s) 307
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 2 cut(s) 94, 99
BsmFI GGGAC 1 cut(s) 307
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 364
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 3 cut(s) 165, 363, 400
Bsp143I GATC 1 cut(s) 272
BspACI CCGC 2 cut(s) 149, 325
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 1 cut(s) 181
BspLI GGNNCC 2 cut(s) 322, 323
BspMAI CTGCAG 1 cut(s) 496
BspPI GGATC 1 cut(s) 267
BspQI GCTCTTC 1 cut(s) 576
BspT104I TTCGAA 1 cut(s) 379
BspTNI GGTCTC 1 cut(s) 94
BsrDI GCAATG 1 cut(s) 44
BsrI ACTGG 2 cut(s) 37, 633
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 1 cut(s) 272
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 1 cut(s) 576
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 630
BstDEI CTNAG 1 cut(s) 168
BstKTI GATC 1 cut(s) 275
BstMAI GTCTC 2 cut(s) 94, 99
BstMBI GATC 1 cut(s) 272
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 1 cut(s) 282
BstSCI CCNGG 2 cut(s) 280, 297
BstSFI CTRYAG 1 cut(s) 492
BstV1I GCAGC 1 cut(s) 478
BstX2I RGATCY 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 215
BstYI RGATCY 1 cut(s) 272
BsuRI GGCC 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 1 cut(s) 630
Cfr13I GGNCC 1 cut(s) 321
CviAII CATG 1 cut(s) 247
CviJI RGCY 9 cut(s) 135, 163, 172, 204, 302, 361, 398, 467, 628
CviKI_1 RGCY 9 cut(s) 135, 163, 172, 204, 302, 361, 398, 467, 628
DdeI CTNAG 1 cut(s) 168
DpnI GATC 1 cut(s) 274
DpnII GATC 1 cut(s) 272
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Ecl136II GAGCTC 2 cut(s) 163, 361
Eco147I AGGCCT 1 cut(s) 204
Eco24I GRGCYC 3 cut(s) 165, 363, 400
Eco31I GGTCTC 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 321
Eco53kI GAGCTC 2 cut(s) 163, 361
Eco88I CYCGRG 1 cut(s) 364
EcoICRI GAGCTC 2 cut(s) 163, 361
EcoO109I RGGNCCY 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 280
EcoT22I ATGCAT 1 cut(s) 44
EcoT38I GRGCYC 3 cut(s) 165, 363, 400
FaeI CATG 1 cut(s) 250
FaiI YATR 9 cut(s) 50, 210, 248, 255, 341, 435, 522, 603, 614
FaqI GGGAC 1 cut(s) 307
FatI CATG 1 cut(s) 246
FauI CCCGC 1 cut(s) 332
Fnu4HI GCNGC 1 cut(s) 492
FriOI GRGCYC 3 cut(s) 165, 363, 400
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 1 cut(s) 332
GluI GCNGC 1 cut(s) 492
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 298
Hin1II CATG 1 cut(s) 250
HindIII AAGCTT 1 cut(s) 626
HinfI GANTC 4 cut(s) 116, 223, 474, 594
HpaII CCGG 1 cut(s) 298
HphI GGTGA 1 cut(s) 270
Hpy166II GTNNAC 2 cut(s) 30, 100
Hpy188I TCNGA 2 cut(s) 121, 347
Hpy188III TCNNGA 3 cut(s) 60, 364, 511
Hpy8I GTNNAC 2 cut(s) 30, 100
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 3 cut(s) 42, 307, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 1 cut(s) 250
KflI GGGWCCC 1 cut(s) 321
Kzo9I GATC 1 cut(s) 272
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 2 cut(s) 168, 464
LpnPI CCDG 8 cut(s) 18, 73, 159, 267, 294, 311, 369, 524
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 1 cut(s) 607
MaeI CTAG 1 cut(s) 332
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 523, 553
MalI GATC 1 cut(s) 274
MboI GATC 1 cut(s) 272
MboII GAAGA 2 cut(s) 281, 563
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 3 cut(s) 165, 363, 400
MluCI AATT 7 cut(s) 17, 68, 75, 122, 212, 530, 580
MlyI GAGTC 2 cut(s) 232, 483
Mph1103I ATGCAT 1 cut(s) 44
MseI TTAA 2 cut(s) 20, 624
MspA1I CMGCKG 1 cut(s) 149
MspI CCGG 1 cut(s) 298
MspR9I CCNGG 2 cut(s) 282, 299
MunI CAATTG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 169
NciI CCSGG 1 cut(s) 299
NdeII GATC 1 cut(s) 272
NlaIII CATG 1 cut(s) 250
NlaIV GGNNCC 2 cut(s) 322, 323
NsiI ATGCAT 1 cut(s) 44
NspV TTCGAA 1 cut(s) 379
PaeR7I CTCGAG 1 cut(s) 364
PceI AGGCCT 1 cut(s) 204
PciSI GCTCTTC 1 cut(s) 576
PfeI GAWTC 2 cut(s) 116, 594
PkrI GCNGC 1 cut(s) 493
PleI GAGTC 2 cut(s) 231, 482
PpsI GAGTC 2 cut(s) 231, 482
PpuMI RGGWCCY 1 cut(s) 321
Psp124BI GAGCTC 2 cut(s) 165, 363
Psp5II RGGWCCY 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 322, 323
PspPI GGNCC 1 cut(s) 321
PspPPI RGGWCCY 1 cut(s) 321
PstI CTGCAG 1 cut(s) 496
PsuI RGATCY 1 cut(s) 272
SacI GAGCTC 2 cut(s) 165, 363
SapI GCTCTTC 1 cut(s) 576
SaqAI TTAA 2 cut(s) 20, 624
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 1 cut(s) 272
Sau96I GGNCC 1 cut(s) 321
SchI GAGTC 2 cut(s) 232, 483
ScrFI CCNGG 2 cut(s) 282, 299
SduI GDGCHC 3 cut(s) 165, 363, 400
SetI ASST 9 cut(s) 26, 90, 132, 165, 192, 283, 363, 469, 630
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 1 cut(s) 492
Sfr274I CTCGAG 1 cut(s) 364
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 1 cut(s) 321
SlaI CTCGAG 1 cut(s) 364
SmlI CTYRAG 1 cut(s) 364
SmoI CTYRAG 1 cut(s) 364
SpeI ACTAGT 1 cut(s) 331
Sse9I AATT 7 cut(s) 17, 68, 75, 122, 212, 530, 580
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 2 cut(s) 149, 325
SspMI CTAG 1 cut(s) 332
SstI GAGCTC 2 cut(s) 165, 363
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 280, 297
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 365, 379
TasI AATT 7 cut(s) 17, 68, 75, 122, 212, 530, 580
TfiI GAWTC 2 cut(s) 116, 594
Tru1I TTAA 2 cut(s) 20, 624
Tru9I TTAA 2 cut(s) 20, 624
TscAI CASTG 1 cut(s) 37
TseI GCWGC 1 cut(s) 491
TspDTI ATGAA 3 cut(s) 143, 263, 601
TspRI CASTG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 3 cut(s) 68, 212, 530
XhoI CTCGAG 1 cut(s) 364
XspI CTAG 1 cut(s) 332
Zsp2I ATGCAT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.