pycom05g07250

Protein of unknown function (DUF565)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012174
Physical Location & Seq
Reverse (-)
10213806 .. 10214273
468 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07250.1

Sequence Viewer

Length: 468 bp
ATGTCACATTCTTTGAGTTTGGCCACTACAGGCCTTTACAAATTTGGCTTGAGTCCTCAAACCAGAGTTTCTACCGGGAAATATGGAGTCTCTACTGTGAGATCCTCACCTGGGTTTTTTCGTGTCCGAGCTGTTCAAGAGGGTGGAGGGTCTCGAAGACTAGTTGATATCATCAGAACTGTGCCAGAACTCTCGAGGAACTATTTTAGAAGTCCATCTCGCAGGGCTCTCTTCGGTGGAATCTCTTTGTTGGGTGGCTTTTATGTGGCACAAACGATATCTTTGTCATTTGGAGCTTTAGGAGTCAACGATGTTATTGCTGCAGTAGTGTGTGTTCTCTTGACAGAGTATGTGACAAAATTTTATTACAGCCGACCAAAAGTAACATTCCCCATCGCTCTTCTCAACAATTTCAAAATGGGATTCACTTATGGCCTGTTCATCGATGCGTTTAAGCTAGCCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

156

Amino Acids

16.96

Weight (kDa)

10.36

Isoelectric Point (pI)

51.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 101 - 155 1.7e-19 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43050
fragaria_vesca FvH4_2g06230
malus_domestica MD05G1079400.v1.1
prunus_persica Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1
pyrus_communis pycom05g07250
rosa_chinensis RchiOBHm_Chr6g0256951
rosa_laevigata RLG00000014664
rosa_multiflora Rmu_co8075354.1_g000001 Rmu_sc0000659.1_g000043
rosa_roxburghii Rroxscaffold_7G00208360
rosa_rugosa Rorug05G0576200
rosa_samantha Rh6AG094800 Rh6BG086000 Rh6CG082100 Rh6DG077800
rosa_wichuraiana Rw6G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 2 cut(s) 41, 359
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 2 cut(s) 137, 415
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 3 cut(s) 131, 296, 457
AluI AGCT 3 cut(s) 131, 296, 457
Alw26I GTCTC 2 cut(s) 94, 156
AlwI GGATC 1 cut(s) 96
Ama87I CYCGRG 1 cut(s) 193
AoxI GGCC 3 cut(s) 21, 31, 433
ApeKI GCWGC 1 cut(s) 320
ApoI RAATTY 2 cut(s) 41, 359
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 99
AsuNHI GCTAGC 1 cut(s) 457
AvaI CYCGRG 1 cut(s) 193
BalI TGGCCA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 307
BccI CCATC 2 cut(s) 223, 401
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BciT130I CCWGG 1 cut(s) 111
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 94, 156
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 2 cut(s) 161, 458
BfmI CTRYAG 2 cut(s) 27, 321
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 2 cut(s) 76, 111
BmeT110I CYCGRG 1 cut(s) 193
BmrFI CCNGG 2 cut(s) 76, 111
BmsI GCATC 1 cut(s) 436
BmtI GCTAGC 1 cut(s) 461
BpiI GAAGAC 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 70
BpuMI CCSGG 1 cut(s) 76
Bsa29I ATCGAT 1 cut(s) 444
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 110
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 462
BseBI CCWGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 110
BseLI CCNNNNNNNGG 1 cut(s) 111
BseNI ACTGG 1 cut(s) 462
BseXI GCAGC 1 cut(s) 307
BshFI GGCC 3 cut(s) 23, 33, 435
BshVI ATCGAT 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 193
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 94, 156
BsnI GGCC 3 cut(s) 23, 33, 435
Bso31I GGTCTC 1 cut(s) 156
BsoBI CYCGRG 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 1 cut(s) 101
BspANI GGCC 3 cut(s) 23, 33, 435
BspDI ATCGAT 1 cut(s) 444
BspMAI CTGCAG 1 cut(s) 325
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 1 cut(s) 96
BspQI GCTCTTC 1 cut(s) 405
BspTNI GGTCTC 1 cut(s) 156
BsrI ACTGG 1 cut(s) 462
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 1 cut(s) 101
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 97, 181
Bst6I CTCTTC 2 cut(s) 236, 405
BstC8I GCNNGC 1 cut(s) 459
BstKTI GATC 1 cut(s) 104
BstMAI GTCTC 2 cut(s) 94, 156
BstMBI GATC 1 cut(s) 101
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 2 cut(s) 74, 109
BstSFI CTRYAG 2 cut(s) 27, 321
BstV1I GCAGC 1 cut(s) 307
BstV2I GAAGAC 1 cut(s) 163
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 3 cut(s) 23, 33, 435
BsuTUI ATCGAT 1 cut(s) 444
BtgZI GCGATG 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 459
ClaI ATCGAT 1 cut(s) 444
DpnI GATC 1 cut(s) 103
DpnII GATC 1 cut(s) 101
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 2 cut(s) 236, 405
EarI CTCTTC 2 cut(s) 236, 405
Eco147I AGGCCT 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 229
Eco31I GGTCTC 1 cut(s) 156
Eco32I GATATC 2 cut(s) 169, 279
Eco88I CYCGRG 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 109
EcoRV GATATC 2 cut(s) 169, 279
EcoT38I GRGCYC 1 cut(s) 229
FaiI YATR 4 cut(s) 84, 264, 351, 432
Fnu4HI GCNGC 1 cut(s) 321
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 2 cut(s) 161, 458
GluI GCNGC 1 cut(s) 321
HaeIII GGCC 3 cut(s) 23, 33, 435
HapII CCGG 1 cut(s) 75
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 5 cut(s) 52, 87, 240, 303, 423
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 99
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 2 cut(s) 128, 176
Hpy188III TCNNGA 4 cut(s) 137, 153, 193, 340
Hpy8I GTNNAC 1 cut(s) 307
HpyCH4III ACNGT 2 cut(s) 97, 181
HpyCH4V TGCA 1 cut(s) 323
Kzo9I GATC 1 cut(s) 101
LguI GCTCTTC 1 cut(s) 405
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 8 cut(s) 15, 76, 88, 96, 123, 198, 208, 449
Lsp1109I GCAGC 1 cut(s) 307
LweI GCATC 1 cut(s) 436
MaeI CTAG 2 cut(s) 161, 458
MaeIII GTNAC 3 cut(s) 3, 352, 382
MalI GATC 1 cut(s) 103
MboI GATC 1 cut(s) 101
MboII GAAGA 3 cut(s) 168, 223, 392
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 229
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 3 cut(s) 41, 359, 409
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 3 cut(s) 61, 96, 312
MnlI CCTC 5 cut(s) 66, 115, 133, 140, 189
Mox20I TGGCCA 1 cut(s) 23
MscI TGGCCA 1 cut(s) 23
MseI TTAA 2 cut(s) 453, 466
Msp20I TGGCCA 1 cut(s) 23
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 2 cut(s) 76, 111
MvaI CCWGG 1 cut(s) 111
NciI CCSGG 1 cut(s) 76
NdeII GATC 1 cut(s) 101
NheI GCTAGC 1 cut(s) 457
NmuCI GTSAC 2 cut(s) 3, 352
PaeR7I CTCGAG 1 cut(s) 193
PceI AGGCCT 1 cut(s) 33
PciSI GCTCTTC 1 cut(s) 405
PfeI GAWTC 2 cut(s) 240, 423
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 3 cut(s) 60, 95, 311
PpsI GAGTC 3 cut(s) 60, 95, 311
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PsrI GAACNNNNNNTAC 2 cut(s) 318, 350
PstI CTGCAG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 405
SaqAI TTAA 2 cut(s) 453, 466
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 1 cut(s) 101
SchI GAGTC 3 cut(s) 61, 96, 312
ScrFI CCNGG 2 cut(s) 76, 111
SduI GDGCHC 1 cut(s) 229
SetI ASST 4 cut(s) 112, 133, 298, 459
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 2 cut(s) 27, 321
Sfr274I CTCGAG 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 193
SmlI CTYRAG 2 cut(s) 49, 193
SmoI CTYRAG 2 cut(s) 49, 193
SpeI ACTAGT 1 cut(s) 160
Sse9I AATT 3 cut(s) 41, 359, 409
SseBI AGGCCT 1 cut(s) 33
SspMI CTAG 2 cut(s) 161, 458
StuI AGGCCT 1 cut(s) 33
StyD4I CCNGG 2 cut(s) 74, 109
TaaI ACNGT 2 cut(s) 97, 181
TaqI TCGA 3 cut(s) 154, 194, 444
TasI AATT 3 cut(s) 41, 359, 409
TfiI GAWTC 2 cut(s) 240, 423
Tru1I TTAA 2 cut(s) 453, 466
Tru9I TTAA 2 cut(s) 453, 466
TseFI GTSAC 2 cut(s) 3, 352
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 2 cut(s) 3, 352
TspDTI ATGAA 1 cut(s) 430
XapI RAATTY 2 cut(s) 41, 359
XhoI CTCGAG 1 cut(s) 193
XspI CTAG 2 cut(s) 161, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.