Rh6BG086000

Protein of unknown function (DUF565)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012174
Physical Location & Seq
Forward (+)
13411737 .. 13414988
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG086000.1

Sequence Viewer

Length: 576 bp
ATGTTTCTGCCTGCTACCTTGTTAAGAAGTATTACTTTTGTTTCTCTTTTTCCAGGATTGGCAGCCACTTTTGTTAGTTTCAGTAGTGGAGTCGTGTTGAATATACCAATGGCTTGTTCATTTAGTTTGCCATCAACAGGCCTCCATAAATTTGGATTGAGTCCTCAAAGCAGAGTTTCGCACATGAAATATGGAGTTTTCACTTTGAGATCCTCACCTGGGTTTCTTCGTGTCCGGGCCATGCAAGAGAATGGAGGGTCCCGCAGACTAGTTGATATAATCAGAACTGTGCCAGAGCTCTCGAGGAACTACTTTCGAAGTCCATCTCGTAGGGCTCTCTTTGGGGGAATATCTTTGTTGGGTGGGTTTTACGTTGCCCAAACAATATCTCTGTCATTTGGAGCTTTAGGGGTCAATGATGTTATTGCTGCAGTTGTGTGTGTTCTCCTGACAGAATATGTAACCAAATTTTATTACAGCAGACCAAAAGTAACATTCCCTGTTGCTCTTCTTAACAATTTCAAAATGGGATTCACTTATGGTCTGTTCATAGATGCTTTTAAGCTTGCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

191

Amino Acids

20.94

Weight (kDa)

10.47

Isoelectric Point (pI)

63.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 137 - 191 2.3e-19 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43050
fragaria_vesca FvH4_2g06230
malus_domestica MD05G1079400.v1.1
prunus_persica Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1
pyrus_communis pycom05g07250
rosa_chinensis RchiOBHm_Chr6g0256951
rosa_laevigata RLG00000014664
rosa_multiflora Rmu_co8075354.1_g000001 Rmu_sc0000659.1_g000043
rosa_roxburghii Rroxscaffold_7G00208360
rosa_rugosa Rorug05G0576200
rosa_samantha Rh6AG094800 Rh6BG086000 Rh6CG082100 Rh6DG077800
rosa_wichuraiana Rw6G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 262
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 2 cut(s) 149, 467
AfiI CCNNNNNNNGG 2 cut(s) 137, 219
AgsI TTSAA 2 cut(s) 100, 523
AhlI ACTAGT 1 cut(s) 268
AjnI CCWGG 2 cut(s) 52, 217
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
AluBI AGCT 3 cut(s) 298, 404, 565
AluI AGCT 3 cut(s) 298, 404, 565
Alw21I GWGCWC 1 cut(s) 300
AlwI GGATC 1 cut(s) 204
Ama87I CYCGRG 1 cut(s) 301
AoxI GGCC 2 cut(s) 139, 237
ApeKI GCWGC 2 cut(s) 62, 428
ApoI RAATTY 2 cut(s) 149, 467
AspS9I GGNCC 2 cut(s) 237, 258
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 1 cut(s) 207
AsuII TTCGAA 1 cut(s) 316
AvaI CYCGRG 1 cut(s) 301
AvaII GGWCC 1 cut(s) 258
BanII GRGCYC 2 cut(s) 300, 337
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 74, 415
BccI CCATC 2 cut(s) 139, 331
BciT130I CCWGG 2 cut(s) 54, 219
BcnI CCSGG 1 cut(s) 236
BcuI ACTAGT 1 cut(s) 268
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 429
BisI GCNGC 2 cut(s) 63, 429
BlsI GCNGC 2 cut(s) 64, 430
Bme1390I CCNGG 3 cut(s) 54, 219, 236
Bme18I GGWCC 1 cut(s) 258
BmeT110I CYCGRG 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 237, 258
BmiI GGNNCC 2 cut(s) 259, 260
BmrFI CCNGG 3 cut(s) 54, 219, 236
BmsI GCATC 1 cut(s) 544
Bpu14I TTCGAA 1 cut(s) 316
BpuMI CCSGG 1 cut(s) 236
BsaJI CCNNGG 1 cut(s) 218
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 219
Bse1I ACTGG 1 cut(s) 570
BseBI CCWGG 2 cut(s) 54, 219
BseDI CCNNGG 1 cut(s) 218
BseLI CCNNNNNNNGG 2 cut(s) 137, 219
BseNI ACTGG 1 cut(s) 570
BseXI GCAGC 2 cut(s) 74, 415
BshFI GGCC 2 cut(s) 141, 239
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 301
BsiSI CCGG 1 cut(s) 235
BslFI GGGAC 1 cut(s) 244
BslI CCNNNNNNNGG 2 cut(s) 137, 219
BsmFI GGGAC 1 cut(s) 244
BsnI GGCC 2 cut(s) 141, 239
BsoBI CYCGRG 1 cut(s) 301
Bsp119I TTCGAA 1 cut(s) 316
Bsp1286I GDGCHC 2 cut(s) 300, 337
Bsp143I GATC 1 cut(s) 209
BspACI CCGC 1 cut(s) 262
BspANI GGCC 2 cut(s) 141, 239
BspLI GGNNCC 2 cut(s) 259, 260
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 1 cut(s) 204
BspQI GCTCTTC 1 cut(s) 513
BspT104I TTCGAA 1 cut(s) 316
BsrI ACTGG 1 cut(s) 570
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 54, 219
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 513
BstBI TTCGAA 1 cut(s) 316
BstC8I GCNNGC 2 cut(s) 12, 567
BstKTI GATC 1 cut(s) 212
BstMBI GATC 1 cut(s) 209
BstNI CCWGG 2 cut(s) 54, 219
BstSCI CCNGG 3 cut(s) 52, 217, 234
BstSFI CTRYAG 1 cut(s) 429
BstV1I GCAGC 2 cut(s) 74, 415
BstX2I RGATCY 1 cut(s) 209
BstXI CCANNNNNNTGG 1 cut(s) 152
BstYI RGATCY 1 cut(s) 209
BsuRI GGCC 2 cut(s) 141, 239
Cac8I GCNNGC 2 cut(s) 12, 567
Cfr13I GGNCC 2 cut(s) 237, 258
CviAII CATG 2 cut(s) 184, 241
CviJI RGCY 8 cut(s) 65, 113, 141, 239, 298, 335, 404, 565
CviKI_1 RGCY 8 cut(s) 65, 113, 141, 239, 298, 335, 404, 565
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Ecl136II GAGCTC 1 cut(s) 298
Eco147I AGGCCT 1 cut(s) 141
Eco24I GRGCYC 2 cut(s) 300, 337
Eco47I GGWCC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 301
EcoICRI GAGCTC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 258
EcoRII CCWGG 2 cut(s) 52, 217
EcoT38I GRGCYC 2 cut(s) 300, 337
FaeI CATG 2 cut(s) 187, 244
FaiI YATR 9 cut(s) 104, 147, 185, 192, 242, 278, 459, 540, 551
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 1 cut(s) 244
FatI CATG 2 cut(s) 183, 240
FauI CCCGC 1 cut(s) 269
Fnu4HI GCNGC 2 cut(s) 63, 429
FriOI GRGCYC 2 cut(s) 300, 337
Fsp4HI GCNGC 2 cut(s) 63, 429
FspBI CTAG 1 cut(s) 269
GluI GCNGC 2 cut(s) 63, 429
HaeIII GGCC 2 cut(s) 141, 239
HapII CCGG 1 cut(s) 235
Hin1II CATG 2 cut(s) 187, 244
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 3 cut(s) 90, 160, 531
HpaII CCGG 1 cut(s) 235
HphI GGTGA 1 cut(s) 207
Hpy188I TCNGA 1 cut(s) 284
Hpy188III TCNNGA 2 cut(s) 301, 448
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 2 cut(s) 244, 431
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 2 cut(s) 187, 244
KflI GGGWCCC 1 cut(s) 258
Kzo9I GATC 1 cut(s) 209
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 2 cut(s) 74, 415
LweI GCATC 1 cut(s) 544
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 460, 490
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MboII GAAGA 2 cut(s) 218, 500
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 2 cut(s) 300, 337
MluCI AATT 3 cut(s) 149, 467, 517
MlyI GAGTC 2 cut(s) 99, 169
MnlI CCTC 5 cut(s) 152, 174, 223, 248, 297
MseI TTAA 3 cut(s) 23, 513, 561
MspI CCGG 1 cut(s) 235
MspR9I CCNGG 3 cut(s) 54, 219, 236
MvaI CCWGG 2 cut(s) 54, 219
NciI CCSGG 1 cut(s) 236
NdeII GATC 1 cut(s) 209
NlaIII CATG 2 cut(s) 187, 244
NlaIV GGNNCC 2 cut(s) 259, 260
NspV TTCGAA 1 cut(s) 316
PaeR7I CTCGAG 1 cut(s) 301
PceI AGGCCT 1 cut(s) 141
PciSI GCTCTTC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 531
PfoI TCCNGGA 1 cut(s) 52
PkrI GCNGC 2 cut(s) 64, 430
PleI GAGTC 2 cut(s) 98, 168
PpsI GAGTC 2 cut(s) 98, 168
PpuMI RGGWCCY 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 52, 217
PspGI CCWGG 2 cut(s) 52, 217
PspN4I GGNNCC 2 cut(s) 259, 260
PspPI GGNCC 2 cut(s) 237, 258
PspPPI RGGWCCY 1 cut(s) 258
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 209
SacI GAGCTC 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 3 cut(s) 23, 513, 561
SatI GCNGC 2 cut(s) 63, 429
Sau3AI GATC 1 cut(s) 209
Sau96I GGNCC 2 cut(s) 237, 258
SchI GAGTC 2 cut(s) 99, 169
ScrFI CCNGG 3 cut(s) 54, 219, 236
SduI GDGCHC 2 cut(s) 300, 337
SetI ASST 6 cut(s) 20, 220, 300, 375, 406, 567
SfaNI GCATC 1 cut(s) 544
SfcI CTRYAG 1 cut(s) 429
Sfr274I CTCGAG 1 cut(s) 301
SfuI TTCGAA 1 cut(s) 316
SinI GGWCC 1 cut(s) 258
SlaI CTCGAG 1 cut(s) 301
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
SpeI ACTAGT 1 cut(s) 268
Sse9I AATT 3 cut(s) 149, 467, 517
SseBI AGGCCT 1 cut(s) 141
SsiI CCGC 1 cut(s) 262
SspMI CTAG 1 cut(s) 269
SstI GAGCTC 1 cut(s) 300
StuI AGGCCT 1 cut(s) 141
StyD4I CCNGG 3 cut(s) 52, 217, 234
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 375
TaqI TCGA 2 cut(s) 302, 316
TasI AATT 3 cut(s) 149, 467, 517
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 3 cut(s) 23, 513, 561
Tru9I TTAA 3 cut(s) 23, 513, 561
TseI GCWGC 2 cut(s) 62, 428
TspDTI ATGAA 3 cut(s) 108, 200, 538
VpaK11BI GGWCC 1 cut(s) 258
XapI RAATTY 2 cut(s) 149, 467
XhoI CTCGAG 1 cut(s) 301
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.