FvH4_2g07760

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016255
Physical Location & Seq
Forward (+)
6707223 .. 6708117
895 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07760.t1

Sequence Viewer

Length: 480 bp
ATGGGTATTTGTGCATCTACTCAGTGTTTGAAGGAAGGTGGAAGAATTGTAAATGGTTCAAGCACAGTAAAGATCGTTCACTTGGACGGAAAGCTACAAGAGCTCAGGAACCCAGTGAAAGCCTCCCACATTCTCTCAAAGAATCCCAATTGCTTCCTCTGCCCCTCGGAGTCCATGTTCGTCGACACAGACGCCCCTCAAGTTCCTAAAGATGAAGAGCTGCGGCTGGGTCAAATATACTTCCTCATGCCAGATTCTCAGTTAAAAGCACCACTGTCTCTCCAAGACTTGTGTTCACTTGCCATCAAGGCCAGTGTAGCTCTTGCAAAGGGTTCGAACTTATCGGCGATGAGGTCGCCGGACTCTGACATAGTTGGAGGAGGTGGATCACAGCTGGGATGTTGCAAGGTTCCAACTGGGTTTGACACGGTGAGCATGAGTTCAAGGATGAGGCGAGGCAACCAAAGAATTGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

160

Amino Acids

17.04

Weight (kDa)

8.5

Isoelectric Point (pI)

51.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 115 4.1e-28 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016255)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07760
malus_domestica MD10G1084100.v1.1
prunus_persica Prupe.8G107400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262091 RchiOBHm_Chr6g0271961
rosa_laevigata RLG00000014452
rosa_multiflora Rmu_sc0002510.1_g000009 Rmu_ssc0000125.1_g000022
rosa_roxburghii Rroxscaffold_7G00207830
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115100 Rh6BG109100 Rh6CG106500 Rh6DG097000
rosa_wichuraiana Rw6G009990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 183
AciI CCGC 1 cut(s) 223
AclWI GGATC 1 cut(s) 394
AcsI RAATTY 1 cut(s) 473
AcyI GRCGYC 1 cut(s) 192
AgsI TTSAA 4 cut(s) 31, 60, 444, 473
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 5 cut(s) 94, 103, 220, 320, 394
AluI AGCT 5 cut(s) 94, 103, 220, 320, 394
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 282
AlwI GGATC 1 cut(s) 394
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 220
ApoI RAATTY 1 cut(s) 473
AsuHPI GGTGA 1 cut(s) 442
AsuII TTCGAA 1 cut(s) 335
BanII GRGCYC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 207
BccI CCATC 1 cut(s) 311
BcgI CGANNNNNNTGC 2 cut(s) 315, 349
BcoDI GTCTC 1 cut(s) 282
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 221, 224
BlsI GCNGC 2 cut(s) 222, 225
BmiI GGNNCC 2 cut(s) 110, 411
BmrI ACTGGG 2 cut(s) 107, 426
BmsI GCATC 1 cut(s) 23
BmuI ACTGGG 2 cut(s) 107, 426
Bpu10I CCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 264, 294
Bse1I ACTGG 3 cut(s) 113, 312, 421
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 2 cut(s) 404, 453
BseMII CTCAG 3 cut(s) 35, 118, 272
BseNI ACTGG 3 cut(s) 113, 312, 421
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 1 cut(s) 207
BseYI CCCAGC 2 cut(s) 226, 394
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 1 cut(s) 359
BsmAI GTCTC 1 cut(s) 282
BsnI GGCC 1 cut(s) 311
Bsp119I TTCGAA 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 2 cut(s) 72, 386
BspACI CCGC 1 cut(s) 223
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 3 cut(s) 34, 117, 271
BspLI GGNNCC 2 cut(s) 110, 411
BspPI GGATC 1 cut(s) 394
BspQI GCTCTTC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 335
BsrI ACTGG 3 cut(s) 113, 312, 421
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 2 cut(s) 72, 386
BssNI GRCGYC 1 cut(s) 192
Bst4CI ACNGT 3 cut(s) 67, 276, 430
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 192
BstBI TTCGAA 1 cut(s) 335
BstDEI CTNAG 3 cut(s) 21, 104, 258
BstF5I GGATG 2 cut(s) 404, 453
BstKTI GATC 2 cut(s) 75, 389
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 2 cut(s) 72, 386
BstMWI GCNNNNNNNGC 4 cut(s) 100, 159, 308, 317
BstV1I GCAGC 1 cut(s) 207
BsuRI GGCC 1 cut(s) 311
BtgZI GCGATG 1 cut(s) 362
BtsCI GGATG 2 cut(s) 404, 453
BtsIMutI CAGTG 4 cut(s) 29, 120, 272, 319
CseI GACGC 1 cut(s) 200
CviAII CATG 3 cut(s) 175, 247, 436
CviJI RGCY 8 cut(s) 94, 103, 122, 220, 226, 311, 320, 394
CviKI_1 RGCY 8 cut(s) 94, 103, 122, 220, 226, 311, 320, 394
DdeI CTNAG 3 cut(s) 21, 104, 258
DpnI GATC 2 cut(s) 74, 388
DpnII GATC 2 cut(s) 72, 386
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 3 cut(s) 178, 250, 439
FaiI YATR 5 cut(s) 176, 238, 248, 371, 437
FatI CATG 3 cut(s) 174, 246, 435
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 2 cut(s) 221, 224
FokI GGATG 2 cut(s) 411, 460
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 221, 224
GluI GCNGC 2 cut(s) 221, 224
GsaI CCCAGC 2 cut(s) 230, 398
HaeIII GGCC 1 cut(s) 311
HapII CCGG 1 cut(s) 359
HgaI GACGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 3 cut(s) 178, 250, 439
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 4 cut(s) 142, 170, 254, 362
HpaII CCGG 1 cut(s) 359
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 3 cut(s) 79, 184, 296
Hpy188I TCNGA 2 cut(s) 169, 367
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 3 cut(s) 79, 184, 296
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 25, 29
HpyCH4III ACNGT 3 cut(s) 67, 276, 430
HpyCH4V TGCA 3 cut(s) 14, 326, 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 159, 308, 317
HpyF3I CTNAG 3 cut(s) 21, 104, 258
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 3 cut(s) 178, 250, 439
Kzo9I GATC 2 cut(s) 72, 386
LguI GCTCTTC 1 cut(s) 210
LpnPI CCDG 8 cut(s) 91, 126, 212, 264, 325, 372, 380, 402
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 1 cut(s) 23
MalI GATC 2 cut(s) 74, 388
MboI GATC 2 cut(s) 72, 386
MboII GAAGA 2 cut(s) 54, 227
MfeI CAATTG 1 cut(s) 148
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 4 cut(s) 45, 148, 468, 473
MlyI GAGTC 2 cut(s) 179, 356
MmeI TCCRAC 2 cut(s) 355, 437
MseI TTAA 2 cut(s) 263, 478
MspA1I CMGCKG 1 cut(s) 394
MspI CCGG 1 cut(s) 359
MunI CAATTG 1 cut(s) 148
MwoI GCNNNNNNNGC 4 cut(s) 100, 159, 308, 317
NdeII GATC 2 cut(s) 72, 386
NlaIII CATG 3 cut(s) 178, 250, 439
NlaIV GGNNCC 2 cut(s) 110, 411
NspV TTCGAA 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 210
PfeI GAWTC 2 cut(s) 142, 254
PkrI GCNGC 2 cut(s) 222, 225
PleI GAGTC 2 cut(s) 178, 356
PpsI GAGTC 2 cut(s) 178, 356
Psp124BI GAGCTC 1 cut(s) 105
PspFI CCCAGC 2 cut(s) 226, 394
PspN4I GGNNCC 2 cut(s) 110, 411
PsrI GAACNNNNNNTAC 2 cut(s) 60, 92
PvuII CAGCTG 1 cut(s) 394
SacI GAGCTC 1 cut(s) 105
SalI GTCGAC 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 2 cut(s) 263, 478
SatI GCNGC 2 cut(s) 221, 224
Sau3AI GATC 2 cut(s) 72, 386
SchI GAGTC 2 cut(s) 179, 356
SduI GDGCHC 1 cut(s) 105
SetI ASST 9 cut(s) 40, 96, 105, 222, 322, 356, 385, 396, 411
SfaNI GCATC 1 cut(s) 23
SfuI TTCGAA 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 4 cut(s) 45, 148, 468, 473
SsiI CCGC 1 cut(s) 223
SstI GAGCTC 1 cut(s) 105
TaaI ACNGT 3 cut(s) 67, 276, 430
TaqI TCGA 2 cut(s) 183, 335
TasI AATT 4 cut(s) 45, 148, 468, 473
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 2 cut(s) 142, 254
Tru1I TTAA 2 cut(s) 263, 478
Tru9I TTAA 2 cut(s) 263, 478
TscAI CASTG 4 cut(s) 29, 120, 279, 319
TseI GCWGC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 4 cut(s) 29, 120, 279, 319
XapI RAATTY 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.