Rmu_ssc0000125.1_g000022

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000125.1
OG0016255
Physical Location & Seq
Forward (+)
75055 .. 79203
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000125.1_g000022.1.cds

Sequence Viewer

Length: 567 bp
atgactgaggccataccagtaccactgcagaagctccgcggcaaggtggccatcgtcaccggcgccgccagcggcatcggagcagtcactgctcgccatttagcaaatcatggcgctctcgctgtcgtaatcgccgacgtgcaagacagcaaggtcaagatcgttcatttggatgggaagctacaagagctcaggaacccagtgaaaccctcccacattctctcacagaatcccaactgcttcctctgcccctcggagttcatgttcgtcgactcagacgccccccaagttcctaccgatgaagagctgcagctgggtcaaatctacttcctcatgccagtttctcagttaagagcaccactgtctctccaagacttgtgttcacttgccatcaaggccagtgtagctctcgcaaagggttcgagcttatcggcgatgaggtcgccggtttcggacagaaatgtagctgcatcacagctgggatgttgcaaggttccaactgggtttgacatggtgggatcaaagagttcaaggatgaggcgaggcaaccaaagaattgaattttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

188

Amino Acids

20.09

Weight (kDa)

9.1

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016255)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07760
malus_domestica MD10G1084100.v1.1
prunus_persica Prupe.8G107400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262091 RchiOBHm_Chr6g0271961
rosa_laevigata RLG00000014452
rosa_multiflora Rmu_sc0002510.1_g000009 Rmu_ssc0000125.1_g000022
rosa_roxburghii Rroxscaffold_7G00207830
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115100 Rh6BG109100 Rh6CG106500 Rh6DG097000
rosa_wichuraiana Rw6G009990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 270
AccII CGCG 1 cut(s) 39
AciI CCGC 4 cut(s) 37, 39, 66, 72
AclWI GGATC 1 cut(s) 526
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 560
AcyI GRCGYC 2 cut(s) 63, 279
AfaI GTAC 1 cut(s) 21
AfiI CCNNNNNNNGG 1 cut(s) 43
AgsI TTSAA 2 cut(s) 531, 560
AjiI CACGTC 1 cut(s) 139
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 9 cut(s) 34, 181, 190, 307, 313, 407, 426, 467, 478
AluI AGCT 9 cut(s) 34, 181, 190, 307, 313, 407, 426, 467, 478
Alw21I GWGCWC 2 cut(s) 192, 358
Alw26I GTCTC 1 cut(s) 369
AlwI GGATC 1 cut(s) 526
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 3 cut(s) 9, 48, 396
ApeKI GCWGC 3 cut(s) 307, 310, 467
ApoI RAATTY 1 cut(s) 560
AspLEI GCGC 2 cut(s) 65, 116
AsuHPI GGTGA 1 cut(s) 49
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 1 cut(s) 192
Bbv12I GWGCWC 2 cut(s) 192, 358
BbvI GCAGC 3 cut(s) 294, 322, 454
BccI CCATC 3 cut(s) 59, 167, 398
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BcoDI GTCTC 1 cut(s) 369
BfmI CTRYAG 2 cut(s) 26, 308
BfoI RGCGCY 2 cut(s) 66, 117
BglI GCCNNNNNGGC 1 cut(s) 395
BisI GCNGC 6 cut(s) 40, 66, 73, 308, 311, 468
BlsI GCNGC 6 cut(s) 41, 67, 74, 309, 312, 469
BmgBI CACGTC 1 cut(s) 139
BmiI GGNNCC 3 cut(s) 64, 197, 495
BmrI ACTGGG 2 cut(s) 194, 510
BmsI GCATC 2 cut(s) 84, 479
BmuI ACTGGG 2 cut(s) 194, 510
Bpu10I CCTNAGC 1 cut(s) 191
BsaHI GRCGYC 2 cut(s) 63, 279
BsaJI CCNNGG 2 cut(s) 37, 252
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 351, 381
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse118I RCCGGY 2 cut(s) 59, 445
Bse1I ACTGG 5 cut(s) 17, 200, 338, 399, 505
BseDI CCNNGG 2 cut(s) 37, 252
BseGI GGATG 3 cut(s) 178, 488, 540
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMII CTCAG 3 cut(s) 205, 288, 359
BseNI ACTGG 5 cut(s) 17, 200, 338, 399, 505
BseXI GCAGC 3 cut(s) 294, 322, 454
BseYI CCCAGC 2 cut(s) 313, 478
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 11, 50, 398
BshNI GGYRCC 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 192, 358
BsiSI CCGG 2 cut(s) 60, 446
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 369
BsnI GGCC 3 cut(s) 11, 50, 398
Bsp1286I GDGCHC 2 cut(s) 192, 358
Bsp143I GATC 2 cut(s) 159, 518
BspACI CCGC 4 cut(s) 37, 39, 66, 72
BspANI GGCC 3 cut(s) 11, 50, 398
BspCNI CTCAG 3 cut(s) 204, 287, 358
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 3 cut(s) 64, 197, 495
BspMAI CTGCAG 2 cut(s) 30, 312
BspPI GGATC 1 cut(s) 526
BspQI GCTCTTC 1 cut(s) 297
BspT107I GGYRCC 1 cut(s) 62
BsrFI RCCGGY 2 cut(s) 59, 445
BsrI ACTGG 5 cut(s) 17, 200, 338, 399, 505
BssAI RCCGGY 2 cut(s) 59, 445
BssECI CCNNGG 2 cut(s) 37, 252
BssMI GATC 2 cut(s) 159, 518
BssNI GRCGYC 2 cut(s) 63, 279
Bst4CI ACNGT 1 cut(s) 363
Bst6I CTCTTC 1 cut(s) 297
BstACI GRCGYC 2 cut(s) 63, 279
BstAPI GCANNNNNTGC 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 70, 94
BstDEI CTNAG 4 cut(s) 6, 191, 274, 345
BstDSI CCRYGG 1 cut(s) 37
BstF5I GGATG 3 cut(s) 178, 488, 540
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 2 cut(s) 66, 117
BstHHI GCGC 2 cut(s) 65, 116
BstKTI GATC 2 cut(s) 162, 521
BstMAI GTCTC 1 cut(s) 369
BstMBI GATC 2 cut(s) 159, 518
BstMWI GCNNNNNNNGC 6 cut(s) 69, 89, 187, 246, 395, 404
BstSFI CTRYAG 2 cut(s) 26, 308
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 294, 322, 454
BsuRI GGCC 3 cut(s) 11, 50, 398
BtgI CCRYGG 1 cut(s) 37
BtgZI GCGATG 1 cut(s) 449
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 3 cut(s) 178, 488, 540
BtsI GCAGTG 2 cut(s) 23, 87
BtsIMutI CAGTG 5 cut(s) 23, 87, 207, 359, 406
Cac8I GCNNGC 2 cut(s) 70, 94
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 2 cut(s) 65, 116
Cfr10I RCCGGY 2 cut(s) 59, 445
Cfr42I CCGCGG 1 cut(s) 40
CseI GACGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 20
CviAII CATG 4 cut(s) 110, 262, 334, 511
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 4 cut(s) 6, 191, 274, 345
DinI GGCGCC 1 cut(s) 64
DpnI GATC 2 cut(s) 161, 520
DpnII GATC 2 cut(s) 159, 518
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 190
EcoT38I GRGCYC 1 cut(s) 192
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
FaeI CATG 4 cut(s) 113, 265, 337, 514
FaiI YATR 5 cut(s) 14, 111, 263, 335, 512
FatI CATG 4 cut(s) 109, 261, 333, 510
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 6 cut(s) 40, 66, 73, 308, 311, 468
FokI GGATG 3 cut(s) 185, 495, 547
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 6 cut(s) 40, 66, 73, 308, 311, 468
GlaI GCGC 2 cut(s) 64, 115
GluI GCNGC 6 cut(s) 40, 66, 73, 308, 311, 468
GsaI CCCAGC 2 cut(s) 317, 482
HaeII RGCGCY 2 cut(s) 66, 117
HaeIII GGCC 3 cut(s) 11, 50, 398
HapII CCGG 2 cut(s) 60, 446
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 65, 116
Hin1I GRCGYC 2 cut(s) 63, 279
Hin1II CATG 4 cut(s) 113, 265, 337, 514
Hin6I GCGC 2 cut(s) 63, 114
HinP1I GCGC 2 cut(s) 63, 114
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 2 cut(s) 229, 272
HpaII CCGG 2 cut(s) 60, 446
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 271, 383
Hpy188I TCNGA 4 cut(s) 80, 256, 277, 454
Hpy188III TCNNGA 2 cut(s) 157, 193
Hpy8I GTNNAC 2 cut(s) 271, 383
Hpy99I CGWCG 2 cut(s) 140, 272
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 5 cut(s) 28, 142, 310, 470, 489
HpyF10VI GCNNNNNNNGC 6 cut(s) 69, 89, 187, 246, 395, 404
HpyF3I CTNAG 4 cut(s) 6, 191, 274, 345
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 2 cut(s) 63, 279
Hsp92II CATG 4 cut(s) 113, 265, 337, 514
HspAI GCGC 2 cut(s) 63, 114
KasI GGCGCC 1 cut(s) 62
KspI CCGCGG 1 cut(s) 40
Kzo9I GATC 2 cut(s) 159, 518
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 39, 80
Lsp1109I GCAGC 3 cut(s) 294, 322, 454
LweI GCATC 2 cut(s) 84, 479
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 55, 85
MalI GATC 2 cut(s) 161, 520
MboI GATC 2 cut(s) 159, 518
MboII GAAGA 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 192, 358
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 2 cut(s) 555, 560
MluNI TGGCCA 1 cut(s) 50
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 7 cut(s) 220, 254, 262, 341, 432, 531, 536
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
MseI TTAA 2 cut(s) 350, 565
MslI CAYNNNNRTG 1 cut(s) 171
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 4 cut(s) 39, 72, 313, 478
MspI CCGG 2 cut(s) 60, 446
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 6 cut(s) 69, 89, 187, 246, 395, 404
NarI GGCGCC 1 cut(s) 63
NdeII GATC 2 cut(s) 159, 518
NlaIII CATG 4 cut(s) 113, 265, 337, 514
NlaIV GGNNCC 3 cut(s) 64, 197, 495
NmuCI GTSAC 2 cut(s) 55, 85
PciSI GCTCTTC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PfeI GAWTC 1 cut(s) 229
PkrI GCNGC 6 cut(s) 41, 67, 74, 309, 312, 469
PleI GAGTC 1 cut(s) 266
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 266
Psp124BI GAGCTC 1 cut(s) 192
PspFI CCCAGC 2 cut(s) 313, 478
PspN4I GGNNCC 3 cut(s) 64, 197, 495
PstI CTGCAG 2 cut(s) 30, 312
PstNI CAGNNNCTG 1 cut(s) 89
PvuII CAGCTG 2 cut(s) 313, 478
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 171
SacI GAGCTC 1 cut(s) 192
SacII CCGCGG 1 cut(s) 40
SalI GTCGAC 1 cut(s) 269
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 2 cut(s) 350, 565
SatI GCNGC 6 cut(s) 40, 66, 73, 308, 311, 468
Sau3AI GATC 2 cut(s) 159, 518
SchI GAGTC 1 cut(s) 266
SduI GDGCHC 2 cut(s) 192, 358
SfaNI GCATC 2 cut(s) 84, 479
SfcI CTRYAG 2 cut(s) 26, 308
SfoI GGCGCC 1 cut(s) 64
Sfr303I CCGCGG 1 cut(s) 40
SgrAI CRCCGGYG 1 cut(s) 59
SgrBI CCGCGG 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 171
Sse9I AATT 2 cut(s) 555, 560
SsiI CCGC 4 cut(s) 37, 39, 66, 72
SspDI GGCGCC 1 cut(s) 62
SstI GAGCTC 1 cut(s) 192
TaaI ACNGT 1 cut(s) 363
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 270, 422
TasI AATT 2 cut(s) 555, 560
TauI GCSGC 3 cut(s) 42, 68, 75
TfiI GAWTC 1 cut(s) 229
Tru1I TTAA 2 cut(s) 350, 565
Tru9I TTAA 2 cut(s) 350, 565
TscAI CASTG 5 cut(s) 30, 94, 207, 366, 406
TseFI GTSAC 2 cut(s) 55, 85
TseI GCWGC 3 cut(s) 307, 310, 467
Tsp45I GTSAC 2 cut(s) 55, 85
TspDTI ATGAA 3 cut(s) 155, 250, 315
TspRI CASTG 5 cut(s) 30, 94, 207, 366, 406
XapI RAATTY 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.