Rroxscaffold_7G00207830

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0016255
Physical Location & Seq
Forward (+)
57799061 .. 57799991
931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00207830.1

Sequence Viewer

Length: 561 bp
ATGCCAATGGACGGATTTGGCGGCAATAAGACCATCGGGAAGAATGTTATACGAATTAATGCGTGTGAGTTGGGAGATTATACGCATCTCCAATCCAGTGTACTAGTTTGCTTGAAGGAAGGTGGAAGAATTGTAAATGGTTCAAGCACAGTCAAGATCGTTCATTTGGATGGGAAGCTACAAGAGCTCAGGAACCCAGTGAAAGCCTCCCACATTCTCTCACAGAATCTCAACTGCTTCCTCTGCCCCTCGGAGTTCATGTTCGTCGACTCGGACGCCCCTCAAGTTCCTACCGATGAAGAGCTGCAGCTGGGTCAAATCTACTTCCTCATGCCAGTTTCCCAGTTAAGAGCACCACTGTGTCTCCAAGACTTGTGTTCACTTGCCATCAAGGCCAGTGTAGCTCTCGCAAAGGGTTCGAGCTTATCGGCAATGAGGTCGCCGGTTTCGGACAGAAATGTAGCTGCATCACAGCTGGGATGTTGCAAGGTTCCAACTGGGTTTGACATGGTGGGATCGATCAATGAGTTCAAGGATGAGGCGAGGCAACCAAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

186

Amino Acids

20.09

Weight (kDa)

6.8

Isoelectric Point (pI)

56.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 42 - 147 5.3e-24 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016255)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07760
malus_domestica MD10G1084100.v1.1
prunus_persica Prupe.8G107400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262091 RchiOBHm_Chr6g0271961
rosa_laevigata RLG00000014452
rosa_multiflora Rmu_sc0002510.1_g000009 Rmu_ssc0000125.1_g000022
rosa_roxburghii Rroxscaffold_7G00207830
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115100 Rh6BG109100 Rh6CG106500 Rh6DG097000
rosa_wichuraiana Rw6G009990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 267
AciI CCGC 1 cut(s) 21
AclWI GGATC 1 cut(s) 523
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 102
AfiI CCNNNNNNNGG 1 cut(s) 11
AgsI TTSAA 3 cut(s) 115, 144, 532
AhlI ACTAGT 1 cut(s) 103
AleI CACNNNNGTG 1 cut(s) 358
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 8 cut(s) 178, 187, 304, 310, 404, 423, 464, 475
AluI AGCT 8 cut(s) 178, 187, 304, 310, 404, 423, 464, 475
Alw21I GWGCWC 2 cut(s) 189, 355
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 1 cut(s) 523
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 3 cut(s) 304, 307, 464
AseI ATTAAT 1 cut(s) 57
BanII GRGCYC 1 cut(s) 189
Bbv12I GWGCWC 2 cut(s) 189, 355
BbvI GCAGC 3 cut(s) 291, 319, 451
BccI CCATC 3 cut(s) 41, 164, 395
BcgI CGANNNNNNTGC 4 cut(s) 399, 420, 433, 454
BcoDI GTCTC 1 cut(s) 368
BcuI ACTAGT 1 cut(s) 103
BfaI CTAG 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 305
BglI GCCNNNNNGGC 1 cut(s) 392
BisI GCNGC 4 cut(s) 22, 305, 308, 465
BlsI GCNGC 4 cut(s) 23, 306, 309, 466
BmiI GGNNCC 2 cut(s) 194, 492
BmrI ACTGGG 3 cut(s) 191, 337, 507
BmsI GCATC 2 cut(s) 94, 476
BmuI ACTGGG 3 cut(s) 191, 337, 507
Bpu10I CCTNAGC 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 267
Bsa29I ATCGAT 1 cut(s) 518
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 249
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 348, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 11
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 6 cut(s) 96, 197, 335, 343, 396, 502
Bse3DI GCAATG 1 cut(s) 438
BseCI ATCGAT 1 cut(s) 518
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 3 cut(s) 175, 485, 541
BseLI CCNNNNNNNGG 1 cut(s) 11
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 202
BseNI ACTGG 6 cut(s) 96, 197, 335, 343, 396, 502
BseXI GCAGC 3 cut(s) 291, 319, 451
BseYI CCCAGC 2 cut(s) 310, 475
BshFI GGCC 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 518
BsiHKAI GWGCWC 2 cut(s) 189, 355
BsiSI CCGG 1 cut(s) 443
BslI CCNNNNNNNGG 1 cut(s) 11
BsmAI GTCTC 1 cut(s) 368
BsnI GGCC 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 189, 355
Bsp143I GATC 3 cut(s) 156, 515, 519
BspACI CCGC 1 cut(s) 21
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 1 cut(s) 201
BspDI ATCGAT 1 cut(s) 518
BspLI GGNNCC 2 cut(s) 194, 492
BspMAI CTGCAG 1 cut(s) 309
BspPI GGATC 1 cut(s) 523
BspQI GCTCTTC 1 cut(s) 294
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 442
BsrI ACTGG 6 cut(s) 96, 197, 335, 343, 396, 502
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 3 cut(s) 156, 515, 519
BssNI GRCGYC 1 cut(s) 276
Bst4CI ACNGT 2 cut(s) 151, 360
Bst6I CTCTTC 1 cut(s) 294
BstACI GRCGYC 1 cut(s) 276
BstDEI CTNAG 1 cut(s) 188
BstF5I GGATG 3 cut(s) 175, 485, 541
BstKTI GATC 3 cut(s) 159, 518, 522
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 3 cut(s) 156, 515, 519
BstMWI GCNNNNNNNGC 4 cut(s) 184, 243, 392, 401
BstSFI CTRYAG 1 cut(s) 305
BstV1I GCAGC 3 cut(s) 291, 319, 451
Bsu15I ATCGAT 1 cut(s) 518
BsuRI GGCC 1 cut(s) 395
BsuTUI ATCGAT 1 cut(s) 518
BtsCI GGATG 3 cut(s) 175, 485, 541
BtsIMutI CAGTG 4 cut(s) 103, 204, 356, 403
Cfr10I RCCGGY 1 cut(s) 442
ClaI ATCGAT 1 cut(s) 518
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 101
CviAII CATG 3 cut(s) 259, 331, 508
CviQI GTAC 1 cut(s) 101
DdeI CTNAG 1 cut(s) 188
DpnI GATC 3 cut(s) 158, 517, 521
DpnII GATC 3 cut(s) 156, 515, 519
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Ecl136II GAGCTC 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 189
Eco53kI GAGCTC 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 3 cut(s) 262, 334, 511
FaiI YATR 5 cut(s) 50, 81, 260, 332, 509
FatI CATG 3 cut(s) 258, 330, 507
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 4 cut(s) 22, 305, 308, 465
FokI GGATG 3 cut(s) 182, 492, 548
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 4 cut(s) 22, 305, 308, 465
FspBI CTAG 1 cut(s) 104
GluI GCNGC 4 cut(s) 22, 305, 308, 465
GsaI CCCAGC 2 cut(s) 314, 479
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 443
HgaI GACGC 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 3 cut(s) 262, 334, 511
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 2 cut(s) 226, 269
HpaII CCGG 1 cut(s) 443
Hpy166II GTNNAC 3 cut(s) 101, 268, 380
Hpy188I TCNGA 3 cut(s) 253, 274, 451
Hpy188III TCNNGA 3 cut(s) 37, 154, 190
Hpy8I GTNNAC 3 cut(s) 101, 268, 380
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 109, 113
HpyCH4III ACNGT 2 cut(s) 151, 360
HpyCH4V TGCA 3 cut(s) 307, 467, 486
HpyF10VI GCNNNNNNNGC 4 cut(s) 184, 243, 392, 401
HpyF3I CTNAG 1 cut(s) 188
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 3 cut(s) 262, 334, 511
Kzo9I GATC 3 cut(s) 156, 515, 519
LguI GCTCTTC 1 cut(s) 294
Lsp1109I GCAGC 3 cut(s) 291, 319, 451
LweI GCATC 2 cut(s) 94, 476
MaeI CTAG 1 cut(s) 104
MalI GATC 3 cut(s) 158, 517, 521
MboI GATC 3 cut(s) 156, 515, 519
MboII GAAGA 3 cut(s) 52, 138, 311
MhlI GDGCHC 2 cut(s) 189, 355
MluCI AATT 3 cut(s) 54, 129, 556
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 518
MnlI CCTC 8 cut(s) 217, 251, 259, 291, 338, 429, 532, 537
MseI TTAA 2 cut(s) 57, 347
MslI CAYNNNNRTG 2 cut(s) 168, 358
MspA1I CMGCKG 2 cut(s) 310, 475
MspI CCGG 1 cut(s) 443
MwoI GCNNNNNNNGC 4 cut(s) 184, 243, 392, 401
NdeII GATC 3 cut(s) 156, 515, 519
NlaIII CATG 3 cut(s) 262, 334, 511
NlaIV GGNNCC 2 cut(s) 194, 492
OliI CACNNNNGTG 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 4 cut(s) 23, 306, 309, 466
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PshBI ATTAAT 1 cut(s) 57
Psp124BI GAGCTC 1 cut(s) 189
PspFI CCCAGC 2 cut(s) 310, 475
PspN4I GGNNCC 2 cut(s) 194, 492
PstI CTGCAG 1 cut(s) 309
PvuII CAGCTG 2 cut(s) 310, 475
RsaI GTAC 1 cut(s) 102
RsaNI GTAC 1 cut(s) 101
RseI CAYNNNNRTG 2 cut(s) 168, 358
SacI GAGCTC 1 cut(s) 189
SalI GTCGAC 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 294
SaqAI TTAA 2 cut(s) 57, 347
SatI GCNGC 4 cut(s) 22, 305, 308, 465
Sau3AI GATC 3 cut(s) 156, 515, 519
SchI GAGTC 1 cut(s) 263
SduI GDGCHC 2 cut(s) 189, 355
SfaNI GCATC 2 cut(s) 94, 476
SfcI CTRYAG 1 cut(s) 305
SmiMI CAYNNNNRTG 2 cut(s) 168, 358
SmlI CTYRAG 1 cut(s) 282
SmoI CTYRAG 1 cut(s) 282
SpeI ACTAGT 1 cut(s) 103
Sse9I AATT 3 cut(s) 54, 129, 556
SsiI CCGC 1 cut(s) 21
SspMI CTAG 1 cut(s) 104
SstI GAGCTC 1 cut(s) 189
TaaI ACNGT 2 cut(s) 151, 360
TaqI TCGA 3 cut(s) 267, 419, 518
TasI AATT 3 cut(s) 54, 129, 556
TatI WGTACW 1 cut(s) 100
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 2 cut(s) 57, 347
Tru9I TTAA 2 cut(s) 57, 347
TscAI CASTG 4 cut(s) 103, 204, 363, 403
TseI GCWGC 3 cut(s) 304, 307, 464
TspDTI ATGAA 3 cut(s) 152, 247, 312
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 4 cut(s) 103, 204, 363, 403
VspI ATTAAT 1 cut(s) 57
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.