MD10G1084100.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016255
Physical Location & Seq
Reverse (-)
12354147 .. 12354632
486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1084100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGGTAACATCTGTGCATCTTCTCAATTCACAAGTAATGGTGGAAGACTTGTAAATAGGTCACCCACTGCAAAGATCATTCACTTGGATGGAAAACTACAAGAGCTTAGGCATCCAGTTCAAGCAACCCATGTCCTCTCCCAAAACCCTAACTGCTTCCTTTGCACCTCAGAGTCCATGGATGTAGATTCATACGCTCCCCAAGTTCCCGAAGACGAAGAGCTACAACTAGGTCAAATCTACTTCCTCATGCCACTTTCTCAGACCAAGTCGTCACTTTCTCTCCAAGACTTGTGTTCTCTGGCCATCAAGGCCAGTGCAGCCCTTGCCAATCCTTTGAAAGCACGGCGTGTCGCATCGTCAGGCAGGTCACCAGCTTCAGACAGAAATGGACAAAATGTTGGTGCTTGTAAAGTTCCTACTGGCATTGACATAGTTGGAATGGAAACAAGGGTAGGGCGAGGAAACCAAAGAATTGAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

162

Amino Acids

17.29

Weight (kDa)

8.33

Isoelectric Point (pI)

60.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 128 1.8e-29 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016255)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07760
malus_domestica MD10G1084100.v1.1
prunus_persica Prupe.8G107400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262091 RchiOBHm_Chr6g0271961
rosa_laevigata RLG00000014452
rosa_multiflora Rmu_sc0002510.1_g000009 Rmu_ssc0000125.1_g000022
rosa_roxburghii Rroxscaffold_7G00207830
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115100 Rh6BG109100 Rh6CG106500 Rh6DG097000
rosa_wichuraiana Rw6G009990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 271
Acc36I ACCTGC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 303
AcuI CTGAAG 1 cut(s) 363
AdeI CACNNNGTG 1 cut(s) 350
AgsI TTSAA 3 cut(s) 122, 340, 479
AluBI AGCT 3 cut(s) 106, 223, 377
AluI AGCT 3 cut(s) 106, 223, 377
AoxI GGCC 2 cut(s) 303, 312
ApeKI GCWGC 1 cut(s) 320
AsuHPI GGTGA 2 cut(s) 54, 363
BalI TGGCCA 1 cut(s) 305
BbsI GAAGAC 2 cut(s) 52, 219
BbvI GCAGC 1 cut(s) 332
BccI CCATC 2 cut(s) 83, 314
BceAI ACGGC 1 cut(s) 362
BfaI CTAG 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 357
BglI GCCNNNNNGGC 1 cut(s) 311
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
BmsI GCATC 3 cut(s) 26, 121, 365
BpiI GAAGAC 2 cut(s) 52, 219
Bpu10I CCTNAGC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bse1I ACTGG 3 cut(s) 116, 315, 427
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 3 cut(s) 94, 112, 187
BseMII CTCAG 2 cut(s) 183, 275
BseNI ACTGG 3 cut(s) 116, 315, 427
BseXI GCAGC 1 cut(s) 332
BsgI GTGCAG 1 cut(s) 339
BshFI GGCC 2 cut(s) 305, 314
BsnI GGCC 2 cut(s) 305, 314
Bsp143I GATC 1 cut(s) 75
Bsp19I CCATGG 1 cut(s) 177
BspANI GGCC 2 cut(s) 305, 314
BspCNI CTCAG 2 cut(s) 182, 274
BspMI ACCTGC 1 cut(s) 357
BspQI GCTCTTC 1 cut(s) 213
BsrI ACTGG 3 cut(s) 116, 315, 427
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 177
Bst6I CTCTTC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 326
BstDEI CTNAG 3 cut(s) 107, 169, 261
BstDSI CCRYGG 1 cut(s) 177
BstEII GGTNACC 2 cut(s) 60, 369
BstF5I GGATG 3 cut(s) 94, 112, 187
BstKTI GATC 1 cut(s) 78
BstMBI GATC 1 cut(s) 75
BstMWI GCNNNNNNNGC 4 cut(s) 162, 311, 320, 326
BstPI GGTNACC 2 cut(s) 60, 369
BstV1I GCAGC 1 cut(s) 332
BstV2I GAAGAC 2 cut(s) 52, 219
BsuRI GGCC 2 cut(s) 305, 314
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 94, 112, 187
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 66, 322
BveI ACCTGC 1 cut(s) 357
CviAII CATG 3 cut(s) 131, 178, 250
CviJI RGCY 6 cut(s) 106, 223, 305, 314, 323, 377
CviKI_1 RGCY 6 cut(s) 106, 223, 305, 314, 323, 377
DdeI CTNAG 3 cut(s) 107, 169, 261
DpnI GATC 1 cut(s) 77
DpnII GATC 1 cut(s) 75
DraIII CACNNNGTG 1 cut(s) 350
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 363
Eco91I GGTNACC 2 cut(s) 60, 369
EcoO65I GGTNACC 2 cut(s) 60, 369
EcoT14I CCWWGG 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 3 cut(s) 134, 181, 253
FaiI YATR 5 cut(s) 132, 179, 193, 251, 434
FatI CATG 3 cut(s) 130, 177, 249
Fnu4HI GCNGC 1 cut(s) 321
FokI GGATG 3 cut(s) 99, 101, 194
Fsp4HI GCNGC 1 cut(s) 321
FspBI CTAG 1 cut(s) 230
GluI GCNGC 1 cut(s) 321
HaeIII GGCC 2 cut(s) 305, 314
Hin1II CATG 3 cut(s) 134, 181, 253
HinfI GANTC 2 cut(s) 173, 188
HphI GGTGA 2 cut(s) 54, 363
Hpy188I TCNGA 3 cut(s) 172, 264, 382
Hpy188III TCNNGA 1 cut(s) 209
HpyCH4V TGCA 4 cut(s) 17, 71, 165, 320
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 311, 320, 326
HpyF3I CTNAG 3 cut(s) 107, 169, 261
Hsp92II CATG 3 cut(s) 134, 181, 253
Kzo9I GATC 1 cut(s) 75
LguI GCTCTTC 1 cut(s) 213
LmnI GCTCC 1 cut(s) 202
LpnPI CCDG 7 cut(s) 129, 287, 328, 348, 352, 387, 408
Lsp1109I GCAGC 1 cut(s) 332
LweI GCATC 3 cut(s) 26, 121, 365
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 4 cut(s) 5, 60, 273, 369
MalI GATC 1 cut(s) 77
MboI GATC 1 cut(s) 75
MboII GAAGA 4 cut(s) 12, 57, 224, 230
MlsI TGGCCA 1 cut(s) 305
MluCI AATT 2 cut(s) 26, 474
MluNI TGGCCA 1 cut(s) 305
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 4 cut(s) 146, 178, 257, 455
Mox20I TGGCCA 1 cut(s) 305
MscI TGGCCA 1 cut(s) 305
MseI TTAA 1 cut(s) 484
MslI CAYNNNNRTG 1 cut(s) 87
Msp20I TGGCCA 1 cut(s) 305
MwoI GCNNNNNNNGC 4 cut(s) 162, 311, 320, 326
NcoI CCATGG 1 cut(s) 177
NdeII GATC 1 cut(s) 75
NlaIII CATG 3 cut(s) 134, 181, 253
NmuCI GTSAC 3 cut(s) 60, 273, 369
PciSI GCTCTTC 1 cut(s) 213
PfeI GAWTC 1 cut(s) 188
PflFI GACNNNGTC 1 cut(s) 268
PkrI GCNGC 1 cut(s) 322
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PspEI GGTNACC 2 cut(s) 60, 369
PsyI GACNNNGTC 1 cut(s) 268
RseI CAYNNNNRTG 1 cut(s) 87
SapI GCTCTTC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 484
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 1 cut(s) 75
SchI GAGTC 1 cut(s) 182
SetI ASST 7 cut(s) 62, 108, 170, 225, 235, 371, 379
SfaNI GCATC 3 cut(s) 26, 121, 365
SfiI GGCCNNNNNGGCC 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 87
Sse9I AATT 2 cut(s) 26, 474
SspMI CTAG 1 cut(s) 230
StyI CCWWGG 1 cut(s) 177
TasI AATT 2 cut(s) 26, 474
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 1 cut(s) 484
Tru9I TTAA 1 cut(s) 484
TscAI CASTG 2 cut(s) 73, 322
TseFI GTSAC 3 cut(s) 60, 273, 369
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 3 cut(s) 60, 273, 369
TspDTI ATGAA 1 cut(s) 180
TspRI CASTG 2 cut(s) 73, 322
Tth111I GACNNNGTC 1 cut(s) 268
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.