RLG00000014452

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016255
Physical Location & Seq
Reverse (-)
53324491 .. 53325121
631 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014452

Sequence Viewer

Length: 501 bp
ATGGGTATTTGTGCATCTACTCAGTGCTTGAAGGAAGGTGGAAGAATTGTAAATGGTTCAAGCACAGTCAAGATCGTTCATTTGGATGGCAAGCTACAAGAGCTCAGGAACCCAGTGAAAGCCTCCCACATTCTCTCACAGAATCCCAACTGCTTCCTCTGCCCCTCGGAGTTCATGTTCGTCGACTCGGACGCCCCTCAAGTTCCTACCGATGAAGAGCTGCAGCTGGGTCAAATCTACTTCCTCATGCCAGTTTCTCAATTAAGAGCACCACTGTCTCTCCAAGATTTGTGTTCACTTGCCATCAAGGCCAGTGTAGCTCTCGCAAAGGGTTCGAGCTTATCGGTGATGAGGTCGCCGGTTTCGGACAGAAATGTAGCTGCATCACAGCAGGGATGTTGCAAGCTGTTCGCTGAAAAGCCAGATCATGATGGCATGGGAAGCAAGCAAGAGTCAAGAATAAAGCTCAGAAATTACAGCCAGGAGATAAGAGTACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

167

Amino Acids

18.08

Weight (kDa)

8.17

Isoelectric Point (pI)

56.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 142 5.8e-29 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016255)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g07760
malus_domestica MD10G1084100.v1.1
prunus_persica Prupe.8G107400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0262091 RchiOBHm_Chr6g0271961
rosa_laevigata RLG00000014452
rosa_multiflora Rmu_sc0002510.1_g000009 Rmu_ssc0000125.1_g000022
rosa_roxburghii Rroxscaffold_7G00207830
rosa_rugosa Rorug05G0595200
rosa_samantha Rh6AG115100 Rh6BG109100 Rh6CG106500 Rh6DG097000
rosa_wichuraiana Rw6G009990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 183
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 1 cut(s) 495
AgsI TTSAA 2 cut(s) 31, 60
AjnI CCWGG 1 cut(s) 480
AloI GAACNNNNNNTCC 2 cut(s) 161, 193
AluBI AGCT 9 cut(s) 94, 103, 220, 226, 320, 339, 380, 406, 466
AluI AGCT 9 cut(s) 94, 103, 220, 226, 320, 339, 380, 406, 466
Alw21I GWGCWC 2 cut(s) 105, 271
Alw26I GTCTC 1 cut(s) 282
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 220, 223, 380
AsuHPI GGTGA 1 cut(s) 358
BanII GRGCYC 1 cut(s) 105
Bbv12I GWGCWC 2 cut(s) 105, 271
BbvI GCAGC 3 cut(s) 207, 235, 367
BccI CCATC 3 cut(s) 80, 311, 425
BcgI CGANNNNNNTGC 4 cut(s) 315, 349, 391, 425
BciT130I CCWGG 1 cut(s) 482
BcoDI GTCTC 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 221
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 3 cut(s) 221, 224, 381
BlsI GCNGC 3 cut(s) 222, 225, 382
Bme1390I CCNGG 1 cut(s) 482
BmiI GGNNCC 1 cut(s) 110
BmrFI CCNGG 1 cut(s) 482
BmrI ACTGGG 1 cut(s) 107
BmsI GCATC 2 cut(s) 23, 392
BmuI ACTGGG 1 cut(s) 107
Bpu10I CCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 264, 294
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 3 cut(s) 113, 251, 312
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 2 cut(s) 91, 401
BseMII CTCAG 3 cut(s) 35, 118, 481
BseNI ACTGG 3 cut(s) 113, 251, 312
BseXI GCAGC 3 cut(s) 207, 235, 367
BseYI CCCAGC 1 cut(s) 226
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 105, 271
BsiSI CCGG 1 cut(s) 359
BsmAI GTCTC 1 cut(s) 282
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 105, 271
Bsp143I GATC 2 cut(s) 72, 424
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 3 cut(s) 34, 117, 480
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 1 cut(s) 110
BspMAI CTGCAG 1 cut(s) 225
BspQI GCTCTTC 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 358
BsrI ACTGG 3 cut(s) 113, 251, 312
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 2 cut(s) 72, 424
BssNI GRCGYC 1 cut(s) 192
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 2 cut(s) 67, 276
Bst6I CTCTTC 1 cut(s) 210
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 3 cut(s) 92, 404, 446
BstDEI CTNAG 3 cut(s) 21, 104, 467
BstF5I GGATG 2 cut(s) 91, 401
BstKTI GATC 2 cut(s) 75, 427
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 2 cut(s) 72, 424
BstMWI GCNNNNNNNGC 5 cut(s) 100, 159, 308, 317, 441
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 1 cut(s) 221
BstV1I GCAGC 3 cut(s) 207, 235, 367
BsuRI GGCC 1 cut(s) 311
BtsCI GGATG 2 cut(s) 91, 401
BtsIMutI CAGTG 4 cut(s) 29, 120, 272, 319
Cac8I GCNNGC 3 cut(s) 92, 404, 446
CciI TCATGA 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 358
CseI GACGC 1 cut(s) 200
Csp6I GTAC 1 cut(s) 494
CviAII CATG 4 cut(s) 175, 247, 428, 436
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 3 cut(s) 21, 104, 467
DpnI GATC 2 cut(s) 74, 426
DpnII GATC 2 cut(s) 72, 424
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 105
Eco53kI GAGCTC 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 4 cut(s) 178, 250, 431, 439
FaiI YATR 5 cut(s) 176, 248, 429, 437, 499
FatI CATG 4 cut(s) 174, 246, 427, 435
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 3 cut(s) 221, 224, 381
FokI GGATG 2 cut(s) 98, 408
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 3 cut(s) 221, 224, 381
GluI GCNGC 3 cut(s) 221, 224, 381
GsaI CCCAGC 1 cut(s) 230
HaeIII GGCC 1 cut(s) 311
HapII CCGG 1 cut(s) 359
HgaI GACGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 4 cut(s) 178, 250, 431, 439
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 3 cut(s) 142, 185, 452
HpaII CCGG 1 cut(s) 359
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 2 cut(s) 184, 296
Hpy188I TCNGA 4 cut(s) 169, 190, 367, 470
Hpy188III TCNNGA 4 cut(s) 70, 106, 428, 456
Hpy8I GTNNAC 2 cut(s) 184, 296
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 25, 29
HpyCH4III ACNGT 2 cut(s) 67, 276
HpyCH4V TGCA 4 cut(s) 14, 223, 383, 402
HpyF10VI GCNNNNNNNGC 5 cut(s) 100, 159, 308, 317, 441
HpyF3I CTNAG 3 cut(s) 21, 104, 467
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 4 cut(s) 178, 250, 431, 439
Kzo9I GATC 2 cut(s) 72, 424
LguI GCTCTTC 1 cut(s) 210
Lsp1109I GCAGC 3 cut(s) 207, 235, 367
LweI GCATC 2 cut(s) 23, 392
MalI GATC 2 cut(s) 74, 426
MboI GATC 2 cut(s) 72, 424
MboII GAAGA 2 cut(s) 54, 227
MhlI GDGCHC 2 cut(s) 105, 271
MluCI AATT 3 cut(s) 45, 260, 472
MlyI GAGTC 2 cut(s) 179, 461
MnlI CCTC 6 cut(s) 133, 167, 175, 207, 254, 345
MseI TTAA 1 cut(s) 263
MslI CAYNNNNRTG 1 cut(s) 84
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 482
MvaI CCWGG 1 cut(s) 482
MwoI GCNNNNNNNGC 5 cut(s) 100, 159, 308, 317, 441
NdeII GATC 2 cut(s) 72, 424
NlaIII CATG 4 cut(s) 178, 250, 431, 439
NlaIV GGNNCC 1 cut(s) 110
PagI TCATGA 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 142
PkrI GCNGC 3 cut(s) 222, 225, 382
PleI GAGTC 2 cut(s) 179, 460
PpsI GAGTC 2 cut(s) 179, 460
Psp124BI GAGCTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 480
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 1 cut(s) 480
PspN4I GGNNCC 1 cut(s) 110
PstI CTGCAG 1 cut(s) 225
PvuII CAGCTG 1 cut(s) 226
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
RseI CAYNNNNRTG 1 cut(s) 84
SacI GAGCTC 1 cut(s) 105
SalI GTCGAC 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 3 cut(s) 221, 224, 381
Sau3AI GATC 2 cut(s) 72, 424
SchI GAGTC 2 cut(s) 179, 461
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 2 cut(s) 105, 271
SfaNI GCATC 2 cut(s) 23, 392
SfcI CTRYAG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 3 cut(s) 45, 260, 472
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 480
TaaI ACNGT 2 cut(s) 67, 276
TaqI TCGA 2 cut(s) 183, 335
TasI AATT 3 cut(s) 45, 260, 472
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TscAI CASTG 4 cut(s) 29, 120, 279, 319
TseI GCWGC 3 cut(s) 220, 223, 380
TspDTI ATGAA 3 cut(s) 68, 163, 228
TspRI CASTG 4 cut(s) 29, 120, 279, 319
XmiI GTMKAC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.