FvH4_2g11370

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0018278
Physical Location & Seq
Forward (+)
9922210 .. 9922614
405 bp
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UTR
Exon/CDS
Intron
FvH4_2g11370.t1

Sequence Viewer

Length: 405 bp
ATGTCTTGCAAATCTGAACCACTGAAATCACTGGAGAATCTACGGTCACCAAATACAGATGGTTTTCTGTTTACCAAAATTCTGAAGGCAGAAGATGCAGTTGAAGGAAATGATGATAATGATAGTGAGAGTGATGATGAGAGTGTTATTAGTTGTAAGGAGGATGAAGTGTTTGATGTTCTGTTATTGAGGAATATGGTAAAGATTGAGCGCCGACAGGGAAATAAGGCACGTGCAGAACTCGAGAAGGAAAGGATGGCTGCTGCATCTGCAGCTGAGGAAGCAATGGCCATGATCTTGCGTTTTCAGAACGAGAAGAGTTCTAGCAAAATCCAAGCCAATCATTATCGCCAGATAGCTAAGCAGAAGCGGCAATATGATGAATCAGTAATCCAGTCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

135

Amino Acids

15.24

Weight (kDa)

5.05

Isoelectric Point (pI)

72.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 63 - 134 6.5e-22 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18265
fragaria_vesca FvH4_2g11370
malus_domestica MD10G1055500.v1.1
prunus_persica Prupe.8G073500_v2.0.a1
pyrus_communis pycom10g04250
rosa_chinensis RchiOBHm_Chr6g0270031
rosa_roxburghii Rroxscaffold_7G00198190
rosa_rugosa Rorug06G0052000
rosa_samantha Rh6AG171100
rosa_wichuraiana Rw6G014710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 370
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 78
AcuI CTGAAG 1 cut(s) 104
AcvI CACGTG 1 cut(s) 233
AgsI TTSAA 1 cut(s) 104
AluBI AGCT 2 cut(s) 275, 359
AluI AGCT 2 cut(s) 275, 359
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 3 cut(s) 260, 263, 272
ApoI RAATTY 1 cut(s) 78
AspLEI GCGC 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 39
AvaI CYCGRG 1 cut(s) 242
BalI TGGCCA 1 cut(s) 290
BbrPI CACGTG 1 cut(s) 233
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 3 cut(s) 247, 250, 284
BccI CCATC 2 cut(s) 53, 250
BfaI CTAG 1 cut(s) 324
BfmI CTRYAG 1 cut(s) 270
BfoI RGCGCY 1 cut(s) 214
BisI GCNGC 4 cut(s) 261, 264, 273, 371
BlpI GCTNAGC 1 cut(s) 360
BlsI GCNGC 4 cut(s) 262, 265, 274, 372
BmeT110I CYCGRG 1 cut(s) 242
BmsI GCATC 2 cut(s) 85, 275
BpmI CTGGAG 1 cut(s) 53
Bpu10I CCTNAGC 1 cut(s) 276
Bpu1102I GCTNAGC 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 36, 394
Bse3DI GCAATG 1 cut(s) 291
BseGI GGATG 2 cut(s) 169, 261
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 2 cut(s) 36, 394
BseXI GCAGC 3 cut(s) 247, 250, 284
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 242
BsnI GGCC 1 cut(s) 290
BsoBI CYCGRG 1 cut(s) 242
Bsp143I GATC 1 cut(s) 294
Bsp1720I GCTNAGC 1 cut(s) 360
BspACI CCGC 1 cut(s) 370
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 268
BspMAI CTGCAG 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 291
BsrI ACTGG 2 cut(s) 36, 394
BssMI GATC 1 cut(s) 294
Bst4CI ACNGT 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 95
BstBAI YACGTR 1 cut(s) 233
BstDEI CTNAG 2 cut(s) 276, 360
BstEII GGTNACC 1 cut(s) 45
BstF5I GGATG 2 cut(s) 169, 261
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 1 cut(s) 297
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 5 cut(s) 95, 269, 272, 281, 370
BstPI GGTNACC 1 cut(s) 45
BstSFI CTRYAG 1 cut(s) 270
BstV1I GCAGC 3 cut(s) 247, 250, 284
BsuRI GGCC 1 cut(s) 290
BtsCI GGATG 2 cut(s) 169, 261
BtsIMutI CAGTG 2 cut(s) 20, 29
CfoI GCGC 1 cut(s) 213
CviAII CATG 1 cut(s) 292
CviJI RGCY 5 cut(s) 260, 275, 290, 338, 359
CviKI_1 RGCY 5 cut(s) 260, 275, 290, 338, 359
DdeI CTNAG 2 cut(s) 276, 360
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 311
EarI CTCTTC 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 104
Eco72I CACGTG 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 242
Eco91I GGTNACC 1 cut(s) 45
EcoO65I GGTNACC 1 cut(s) 45
FaeI CATG 1 cut(s) 295
FaiI YATR 3 cut(s) 197, 293, 378
FatI CATG 1 cut(s) 291
Fnu4HI GCNGC 4 cut(s) 261, 264, 273, 371
FokI GGATG 2 cut(s) 176, 268
Fsp4HI GCNGC 4 cut(s) 261, 264, 273, 371
FspBI CTAG 1 cut(s) 324
GlaI GCGC 1 cut(s) 212
GluI GCNGC 4 cut(s) 261, 264, 273, 371
GsuI CTGGAG 1 cut(s) 53
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 1 cut(s) 290
HhaI GCGC 1 cut(s) 213
Hin1II CATG 1 cut(s) 295
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 2 cut(s) 37, 383
HphI GGTGA 1 cut(s) 39
Hpy166II GTNNAC 1 cut(s) 72
Hpy188I TCNGA 3 cut(s) 16, 84, 309
Hpy188III TCNNGA 1 cut(s) 244
Hpy8I GTNNAC 1 cut(s) 72
HpyAV CCTTC 3 cut(s) 79, 98, 241
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 1 cut(s) 232
HpyCH4V TGCA 5 cut(s) 9, 98, 236, 266, 272
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 269, 272, 281, 370
HpyF3I CTNAG 2 cut(s) 276, 360
HpySE526I ACGT 1 cut(s) 232
Hsp92II CATG 1 cut(s) 295
HspAI GCGC 1 cut(s) 211
Kzo9I GATC 1 cut(s) 294
LpnPI CCDG 3 cut(s) 17, 203, 365
Lsp1109I GCAGC 3 cut(s) 247, 250, 284
LweI GCATC 2 cut(s) 85, 275
MaeI CTAG 1 cut(s) 324
MaeII ACGT 1 cut(s) 232
MaeIII GTNAC 1 cut(s) 45
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 2 cut(s) 104, 328
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 1 cut(s) 78
MluNI TGGCCA 1 cut(s) 290
MnlI CCTC 3 cut(s) 154, 183, 271
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 275
MwoI GCNNNNNNNGC 5 cut(s) 95, 269, 272, 281, 370
NdeII GATC 1 cut(s) 294
NlaIII CATG 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 45
PaeR7I CTCGAG 1 cut(s) 242
PfeI GAWTC 2 cut(s) 37, 383
PkrI GCNGC 4 cut(s) 262, 265, 274, 372
PmaCI CACGTG 1 cut(s) 233
PmlI CACGTG 1 cut(s) 233
Ppu21I YACGTR 1 cut(s) 233
PspCI CACGTG 1 cut(s) 233
PspEI GGTNACC 1 cut(s) 45
PstI CTGCAG 1 cut(s) 274
PvuII CAGCTG 1 cut(s) 275
SatI GCNGC 4 cut(s) 261, 264, 273, 371
Sau3AI GATC 1 cut(s) 294
SetI ASST 3 cut(s) 235, 277, 361
SfaNI GCATC 2 cut(s) 85, 275
SfcI CTRYAG 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 242
SlaI CTCGAG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 1 cut(s) 78
SsiI CCGC 1 cut(s) 370
SspMI CTAG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 1 cut(s) 235
TaqI TCGA 1 cut(s) 243
TasI AATT 1 cut(s) 78
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 2 cut(s) 37, 383
TscAI CASTG 2 cut(s) 27, 36
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 3 cut(s) 260, 263, 272
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 2 cut(s) 180, 396
TspRI CASTG 2 cut(s) 27, 36
XapI RAATTY 1 cut(s) 78
XhoI CTCGAG 1 cut(s) 242
XspI CTAG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.