MD10G1055500.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0018278
Physical Location & Seq
Reverse (-)
7545825 .. 7547111
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1055500.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGATGTTCTAGAGGAGGTAGATGCAGATTTGGGAGGTAAATCAAGAGATTTCAGAAATCGGGTTTGTTCGAGGTGTGGTTCTGGTGATGATGTCTGTTCTCCAAAGGTGAAATCATTGGAAACTTCACCAAATGCAGATTGCTTGTTGTTAAGCAAAAATGTCAATGCAGAAGAGAGAGATCATGTCGATATTAACGACGGTCATGGTGATAGCGATGGTGATGATGAAAGTGTTGGTTACGGCGACGATGGGGAATTTGATGTGTTTGCATTGAGAAAAATGGTGAAGGTTGAGCGGCGAAGGAGGAATGAGGCTCGCGTAGAGCTCGAGAAGGAAAGGATGGCTGCTGCATCTGCAGCCGAGGAAGCTATGGCCATGATCCTGCGTCTTCAAAACGAGAAAAGCTGTACTGAAATCCAAGCCAAGCAGTACCGTCAAATGGCGGAGCAGAAGCAGCAGTATGATGAAGTGATGTTCCGATCGATGCAGTGGATTATAATGAAACACGAATCGGAGAGGAGTCTACTGGAGGAGCAACTGAGGCTGTACAGGCAAAAGTTGCAGCAATGTCTTAAGGGTGATGAAACGGCGGCACAATCCCAAGAAGCTGATCCGAGTTCCAATCTAATCGAGAATTGCATGGATGATGGGCTTGACGTAGATCCGTGGGAGCCGGTGCGACCGCACTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

232

Amino Acids

26.36

Weight (kDa)

4.68

Isoelectric Point (pI)

61.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 91 - 181 1.9e-28 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18265
fragaria_vesca FvH4_2g11370
malus_domestica MD10G1055500.v1.1
prunus_persica Prupe.8G073500_v2.0.a1
pyrus_communis pycom10g04250
rosa_chinensis RchiOBHm_Chr6g0270031
rosa_roxburghii Rroxscaffold_7G00198190
rosa_rugosa Rorug06G0052000
rosa_samantha Rh6AG171100
rosa_wichuraiana Rw6G014710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 298
AccI GTMKAC 1 cut(s) 526
AccII CGCG 1 cut(s) 321
AciI CCGC 4 cut(s) 298, 446, 593, 686
AclWI GGATC 3 cut(s) 376, 608, 659
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 1 cut(s) 257
AfaI GTAC 3 cut(s) 412, 434, 551
AfiI CCNNNNNNNGG 1 cut(s) 442
AflII CTTAAG 1 cut(s) 575
AgsI TTSAA 1 cut(s) 395
AluBI AGCT 4 cut(s) 328, 371, 408, 611
AluI AGCT 4 cut(s) 328, 371, 408, 611
Alw21I GWGCWC 1 cut(s) 330
AlwI GGATC 3 cut(s) 376, 608, 659
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 1 cut(s) 375
ApeKI GCWGC 5 cut(s) 347, 350, 359, 457, 565
ApoI RAATTY 1 cut(s) 257
AsuHPI GGTGA 7 cut(s) 98, 120, 121, 221, 233, 298, 593
AvaI CYCGRG 1 cut(s) 329
BalI TGGCCA 1 cut(s) 377
BanII GRGCYC 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 5 cut(s) 334, 337, 371, 469, 577
BccI CCATC 4 cut(s) 212, 245, 337, 644
BceAI ACGGC 2 cut(s) 259, 606
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 1 cut(s) 357
BfrI CTTAAG 1 cut(s) 575
BisI GCNGC 7 cut(s) 299, 348, 351, 360, 458, 566, 594
BlsI GCNGC 7 cut(s) 300, 349, 352, 361, 459, 567, 595
BmeT110I CYCGRG 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 675
BmsI GCATC 3 cut(s) 13, 362, 477
BpiI GAAGAC 1 cut(s) 383
BpmI CTGGAG 1 cut(s) 551
Bsa29I ATCGAT 1 cut(s) 485
BsaJI CCNNGG 2 cut(s) 363, 668
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse118I RCCGGY 1 cut(s) 676
Bse1I ACTGG 1 cut(s) 534
Bse3DI GCAATG 1 cut(s) 575
BseCI ATCGAT 1 cut(s) 485
BseDI CCNNGG 2 cut(s) 363, 668
BseGI GGATG 3 cut(s) 10, 348, 652
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMI GCAATG 1 cut(s) 575
BseMII CTCAG 1 cut(s) 533
BseNI ACTGG 1 cut(s) 534
BseRI GAGGAG 3 cut(s) 29, 535, 548
BseXI GCAGC 5 cut(s) 334, 337, 371, 469, 577
Bsh1236I CGCG 1 cut(s) 321
Bsh1285I CGRYCG 2 cut(s) 485, 686
BshFI GGCC 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 485
BsiEI CGRYCG 2 cut(s) 485, 686
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 1 cut(s) 677
BslI CCNNNNNNNGG 1 cut(s) 442
BsnI GGCC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 330
Bsp1407I TGTACA 1 cut(s) 549
Bsp143I GATC 5 cut(s) 181, 381, 482, 613, 664
BspACI CCGC 4 cut(s) 298, 446, 593, 686
BspANI GGCC 1 cut(s) 377
BspCNI CTCAG 1 cut(s) 534
BspDI ATCGAT 1 cut(s) 485
BspFNI CGCG 1 cut(s) 321
BspLI GGNNCC 1 cut(s) 675
BspMAI CTGCAG 1 cut(s) 361
BspPI GGATC 3 cut(s) 376, 608, 659
BspTI CTTAAG 1 cut(s) 575
BsrBI CCGCTC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 575
BsrFI RCCGGY 1 cut(s) 676
BsrGI TGTACA 1 cut(s) 549
BsrI ACTGG 1 cut(s) 534
BssAI RCCGGY 1 cut(s) 676
BssECI CCNNGG 2 cut(s) 363, 668
BssMI GATC 5 cut(s) 181, 381, 482, 613, 664
Bst4CI ACNGT 2 cut(s) 203, 437
Bst6I CTCTTC 1 cut(s) 168
BstAFI CTTAAG 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 562
BstAUI TGTACA 1 cut(s) 549
BstC8I GCNNGC 1 cut(s) 319
BstDEI CTNAG 1 cut(s) 542
BstDSI CCRYGG 1 cut(s) 668
BstF5I GGATG 3 cut(s) 10, 348, 652
BstFNI CGCG 1 cut(s) 321
BstKTI GATC 5 cut(s) 184, 384, 485, 616, 667
BstMBI GATC 5 cut(s) 181, 381, 482, 613, 664
BstMCI CGRYCG 2 cut(s) 485, 686
BstMWI GCNNNNNNNGC 7 cut(s) 356, 359, 368, 457, 544, 553, 562
BstSFI CTRYAG 1 cut(s) 357
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 5 cut(s) 334, 337, 371, 469, 577
BstV2I GAAGAC 1 cut(s) 383
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
Bsu15I ATCGAT 1 cut(s) 485
BsuRI GGCC 1 cut(s) 377
BsuTUI ATCGAT 1 cut(s) 485
BtgI CCRYGG 1 cut(s) 668
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 3 cut(s) 10, 348, 652
BtsI GCAGTG 1 cut(s) 497
BtsIMutI CAGTG 1 cut(s) 497
Cac8I GCNNGC 1 cut(s) 319
Cfr10I RCCGGY 1 cut(s) 676
ClaI ATCGAT 1 cut(s) 485
CseI GACGC 1 cut(s) 377
Csp6I GTAC 3 cut(s) 411, 433, 550
CviAII CATG 4 cut(s) 185, 206, 379, 643
CviQI GTAC 3 cut(s) 411, 433, 550
DdeI CTNAG 1 cut(s) 542
DpnI GATC 5 cut(s) 183, 383, 484, 615, 666
DpnII GATC 5 cut(s) 181, 381, 482, 613, 664
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
EciI GGCGGA 1 cut(s) 461
Ecl136II GAGCTC 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 330
FaeI CATG 4 cut(s) 188, 209, 382, 646
FaiI YATR 8 cut(s) 186, 207, 374, 380, 465, 500, 644, 694
FatI CATG 4 cut(s) 184, 205, 378, 642
FblI GTMKAC 1 cut(s) 526
Fnu4HI GCNGC 7 cut(s) 299, 348, 351, 360, 458, 566, 594
FokI GGATG 3 cut(s) 17, 355, 659
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 7 cut(s) 299, 348, 351, 360, 458, 566, 594
FspBI CTAG 1 cut(s) 11
GluI GCNGC 7 cut(s) 299, 348, 351, 360, 458, 566, 594
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 1 cut(s) 377
HapII CCGG 1 cut(s) 677
HgaI GACGC 1 cut(s) 377
Hin1II CATG 4 cut(s) 188, 209, 382, 646
HinfI GANTC 2 cut(s) 512, 523
HpaII CCGG 1 cut(s) 677
HphI GGTGA 7 cut(s) 98, 120, 121, 221, 233, 298, 593
Hpy166II GTNNAC 1 cut(s) 527
Hpy188I TCNGA 4 cut(s) 56, 482, 517, 618
Hpy188III TCNNGA 4 cut(s) 11, 45, 331, 634
Hpy8I GTNNAC 1 cut(s) 527
Hpy99I CGWCG 2 cut(s) 203, 251
HpyAV CCTTC 3 cut(s) 283, 297, 328
HpyCH4III ACNGT 2 cut(s) 203, 437
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 9 cut(s) 26, 137, 170, 272, 353, 359, 490, 565, 642
HpyF10VI GCNNNNNNNGC 7 cut(s) 356, 359, 368, 457, 544, 553, 562
HpyF3I CTNAG 1 cut(s) 542
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 4 cut(s) 188, 209, 382, 646
Kzo9I GATC 5 cut(s) 181, 381, 482, 613, 664
LmnI GCTCC 3 cut(s) 448, 535, 673
LpnPI CCDG 5 cut(s) 69, 398, 515, 538, 690
Lsp1109I GCAGC 5 cut(s) 334, 337, 371, 469, 577
LweI GCATC 3 cut(s) 13, 362, 477
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 1 cut(s) 239
MalI GATC 5 cut(s) 183, 383, 484, 615, 666
MbiI CCGCTC 1 cut(s) 298
MboI GATC 5 cut(s) 181, 381, 482, 613, 664
MboII GAAGA 2 cut(s) 185, 383
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 1 cut(s) 330
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 2 cut(s) 257, 637
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 1 cut(s) 532
Mox20I TGGCCA 1 cut(s) 377
MscI TGGCCA 1 cut(s) 377
MseI TTAA 3 cut(s) 152, 195, 576
Msp20I TGGCCA 1 cut(s) 377
MspCI CTTAAG 1 cut(s) 575
MspI CCGG 1 cut(s) 677
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 7 cut(s) 356, 359, 368, 457, 544, 553, 562
NdeII GATC 5 cut(s) 181, 381, 482, 613, 664
NlaIII CATG 4 cut(s) 188, 209, 382, 646
NlaIV GGNNCC 1 cut(s) 675
NmeAIII GCCGAG 1 cut(s) 388
PaeR7I CTCGAG 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 1 cut(s) 512
PkrI GCNGC 7 cut(s) 300, 349, 352, 361, 459, 567, 595
Ple19I CGATCG 1 cut(s) 485
PleI GAGTC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 531
PsiI TTATAA 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 675
PstI CTGCAG 1 cut(s) 361
PsuI RGATCY 1 cut(s) 664
PvuI CGATCG 1 cut(s) 485
RsaI GTAC 3 cut(s) 412, 434, 551
RsaNI GTAC 3 cut(s) 411, 433, 550
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 3 cut(s) 152, 195, 576
SatI GCNGC 7 cut(s) 299, 348, 351, 360, 458, 566, 594
Sau3AI GATC 5 cut(s) 181, 381, 482, 613, 664
SchI GAGTC 1 cut(s) 532
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 3 cut(s) 13, 362, 477
SfcI CTRYAG 1 cut(s) 357
Sfr274I CTCGAG 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 329
SmlI CTYRAG 2 cut(s) 329, 575
SmoI CTYRAG 2 cut(s) 329, 575
Sse9I AATT 2 cut(s) 257, 637
SsiI CCGC 4 cut(s) 298, 446, 593, 686
SspMI CTAG 1 cut(s) 11
SstI GAGCTC 1 cut(s) 330
TaaI ACNGT 2 cut(s) 203, 437
TaiI ACGT 1 cut(s) 663
TaqI TCGA 5 cut(s) 71, 189, 330, 485, 633
TasI AATT 2 cut(s) 257, 637
TatI WGTACW 2 cut(s) 410, 549
TauI GCSGC 2 cut(s) 301, 596
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 3 cut(s) 152, 195, 576
Tru9I TTAA 3 cut(s) 152, 195, 576
TscAI CASTG 1 cut(s) 497
TseI GCWGC 5 cut(s) 347, 350, 359, 457, 565
TspDTI ATGAA 4 cut(s) 243, 483, 518, 600
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 1 cut(s) 497
Vha464I CTTAAG 1 cut(s) 575
XapI RAATTY 1 cut(s) 257
XbaI TCTAGA 1 cut(s) 10
XhoI CTCGAG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 526
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.