Rw6G014710

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0018278
Physical Location & Seq
Reverse (-)
27375099 .. 27376491
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014710.1

Sequence Viewer

Length: 882 bp
ATGCAAAGTTTGGTTCAGTTTTACTTGTTTCTTATTTGTTTTTCTGCTGTTCTTGAGTTTGTTCAAAATTTTCTGGGATTCTTTGGGATGGATTTTGTGTTTGGGGTTTTGTTGTACGGTCGTTTCTCGCCGCTTTTTGTGTCAGGGTTGTCTCTCATGTTCGGGTTTGGTTTGTTATACCATTACAACTATTTGATTAAATTCCTATGTGACAATAGAGCGAAAGCCAGTGACTTTAGTAATGGGTTTTGCTCAAATTGCGGTTCTCATGAAGTTCGTGCTTCAAAGGCCGTGTCTTCCAAATCTGAACCACTGAAATCATTGAAGAATTTACAGTCATCAAATGCAGATGGTTTTCTGTTAACCAAAATTGTAAAGGCAGAAGATGCAATTGAAGGAAAAGATGATAATGAGGGTGGCAATGATGATGAGAATTTTTGTTGTAAGGAGGATGAAGTGTTTGATGTTCTGTCATTGAGGAAATTGGTAAAGATTGAGCGCCGGCGGGGGAATGAGGCACGTGCAGAACTCGAGAAGGAAAGGATGGCTGCTGCATCTGCAGCTGAGGAAGCAATGGCCATGATCTTGCGTCTTCAGAACGAGAAGAGTTCTACTGAAATCCAGGCTAGTCATTATCGCCAGATAGCTGAGCAGAAGCAGCAATATGATGAAGCAGTGATTCAGTCCTTGCAGTGGATTATAATGAAACACGAATCTGAGAGGAGTCAATTGGAAGAGCAACTAAGGCTGGGCAGGCAAATGTGCAATGGTCAGTCTGAGGAAGGTGATCCAAGTAAAAACCTGTTCGATTCCACCAACATGGAAGATGATGGCCTCGAAGATGTCTTTGGTGCACATTGTAGGTATTTTGAATCCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

293

Amino Acids

33.3

Weight (kDa)

4.9

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 158 - 248 4.5e-29 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18265
fragaria_vesca FvH4_2g11370
malus_domestica MD10G1055500.v1.1
prunus_persica Prupe.8G073500_v2.0.a1
pyrus_communis pycom10g04250
rosa_chinensis RchiOBHm_Chr6g0270031
rosa_roxburghii Rroxscaffold_7G00198190
rosa_rugosa Rorug06G0052000
rosa_samantha Rh6AG171100
rosa_wichuraiana Rw6G014710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 701
AciI CCGC 3 cut(s) 131, 261, 505
AclWI GGATC 1 cut(s) 782
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 4 cut(s) 67, 200, 328, 433
AcuI CTGAAG 1 cut(s) 578
AcvI CACGTG 1 cut(s) 521
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 693
AgsI TTSAA 5 cut(s) 65, 285, 325, 395, 872
AjnI CCWGG 1 cut(s) 621
AjuI GAANNNNNNNTTGG 2 cut(s) 831, 863
AluBI AGCT 2 cut(s) 563, 647
AluI AGCT 2 cut(s) 563, 647
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 1 cut(s) 156
Alw44I GTGCAC 1 cut(s) 852
AlwI GGATC 1 cut(s) 782
Ama87I CYCGRG 1 cut(s) 530
AoxI GGCC 3 cut(s) 288, 576, 832
ApaLI GTGCAC 1 cut(s) 852
ApeKI GCWGC 4 cut(s) 548, 551, 560, 658
ApoI RAATTY 4 cut(s) 67, 200, 328, 433
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AspLEI GCGC 1 cut(s) 501
AsuHPI GGTGA 1 cut(s) 797
AvaI CYCGRG 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 856
BalI TGGCCA 1 cut(s) 578
BbrPI CACGTG 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 288, 584
Bbv12I GWGCWC 1 cut(s) 856
BbvCI CCTCAGC 1 cut(s) 564
BbvI GCAGC 4 cut(s) 535, 538, 572, 670
BccI CCATC 4 cut(s) 82, 344, 538, 824
BceAI ACGGC 1 cut(s) 275
BciT130I CCWGG 1 cut(s) 623
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 1 cut(s) 627
BfmI CTRYAG 1 cut(s) 558
BfoI RGCGCY 1 cut(s) 502
BisI GCNGC 5 cut(s) 131, 549, 552, 561, 659
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 5 cut(s) 132, 550, 553, 562, 660
Bme1390I CCNGG 1 cut(s) 623
BmeT110I CYCGRG 1 cut(s) 530
BmrFI CCNGG 1 cut(s) 623
BmsI GCATC 2 cut(s) 376, 563
BpiI GAAGAC 2 cut(s) 288, 584
Bpu10I CCTNAGC 1 cut(s) 564
Bpu1102I GCTNAGC 1 cut(s) 648
BpuEI CTTGAG 1 cut(s) 74
BsaAI YACGTR 1 cut(s) 521
Bsc4I CCNNNNNNNGG 1 cut(s) 693
Bse118I RCCGGY 1 cut(s) 501
Bse1I ACTGG 1 cut(s) 228
Bse3DI GCAATG 3 cut(s) 427, 579, 772
BseBI CCWGG 1 cut(s) 623
BseGI GGATG 3 cut(s) 93, 457, 549
BseLI CCNNNNNNNGG 1 cut(s) 693
BseMI GCAATG 3 cut(s) 427, 579, 772
BseMII CTCAG 4 cut(s) 555, 639, 708, 768
BseNI ACTGG 1 cut(s) 228
BseRI GAGGAG 1 cut(s) 736
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 4 cut(s) 535, 538, 572, 670
BseYI CCCAGC 1 cut(s) 748
BsgI GTGCAG 1 cut(s) 543
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 3 cut(s) 290, 578, 834
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 856
BsiHKCI CYCGRG 1 cut(s) 530
BsiSI CCGG 1 cut(s) 502
BslI CCNNNNNNNGG 1 cut(s) 693
BsmAI GTCTC 1 cut(s) 156
BsnI GGCC 3 cut(s) 290, 578, 834
BsoBI CYCGRG 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 856
Bsp143I GATC 2 cut(s) 582, 787
Bsp1720I GCTNAGC 1 cut(s) 648
BspACI CCGC 3 cut(s) 131, 261, 505
BspANI GGCC 3 cut(s) 290, 578, 834
BspCNI CTCAG 4 cut(s) 556, 640, 709, 769
BspHI TCATGA 1 cut(s) 268
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 1 cut(s) 782
BspQI GCTCTTC 1 cut(s) 729
BsrDI GCAATG 3 cut(s) 427, 579, 772
BsrFI RCCGGY 1 cut(s) 501
BsrI ACTGG 1 cut(s) 228
BssAI RCCGGY 1 cut(s) 501
BssMI GATC 2 cut(s) 582, 787
Bst2UI CCWGG 1 cut(s) 623
Bst4CI ACNGT 2 cut(s) 119, 336
Bst6I CTCTTC 2 cut(s) 599, 729
BstAPI GCANNNNNTGC 1 cut(s) 386
BstBAI YACGTR 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 503, 755
BstDEI CTNAG 5 cut(s) 564, 648, 717, 743, 777
BstF5I GGATG 3 cut(s) 93, 457, 549
BstH2I RGCGCY 1 cut(s) 502
BstHHI GCGC 1 cut(s) 501
BstKTI GATC 2 cut(s) 585, 790
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 582, 787
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 9 cut(s) 258, 287, 386, 557, 560, 569, 658, 745, 754
BstNI CCWGG 1 cut(s) 623
BstSCI CCNGG 1 cut(s) 621
BstSFI CTRYAG 1 cut(s) 558
BstSLI GKGCMC 1 cut(s) 856
BstV1I GCAGC 4 cut(s) 535, 538, 572, 670
BstV2I GAAGAC 2 cut(s) 288, 584
BstXI CCANNNNNNTGG 1 cut(s) 820
BsuRI GGCC 3 cut(s) 290, 578, 834
BtsCI GGATG 3 cut(s) 93, 457, 549
BtsI GCAGTG 2 cut(s) 681, 698
BtsIMutI CAGTG 4 cut(s) 235, 311, 681, 698
Cac8I GCNNGC 2 cut(s) 503, 755
CciI TCATGA 1 cut(s) 268
CfoI GCGC 1 cut(s) 501
Cfr10I RCCGGY 1 cut(s) 501
CseI GACGC 1 cut(s) 578
Csp6I GTAC 1 cut(s) 115
CviAII CATG 4 cut(s) 157, 269, 580, 820
CviJI RGCY 9 cut(s) 227, 290, 548, 563, 578, 626, 647, 748, 834
CviKI_1 RGCY 9 cut(s) 227, 290, 548, 563, 578, 626, 647, 748, 834
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 5 cut(s) 564, 648, 717, 743, 777
DpnI GATC 2 cut(s) 584, 789
DpnII GATC 2 cut(s) 582, 787
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 2 cut(s) 599, 729
EarI CTCTTC 2 cut(s) 599, 729
Eco57I CTGAAG 1 cut(s) 578
Eco72I CACGTG 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 621
FaeI CATG 4 cut(s) 160, 272, 583, 823
FaiI YATR 8 cut(s) 158, 178, 208, 270, 581, 666, 701, 821
FatI CATG 4 cut(s) 156, 268, 579, 819
FauI CCCGC 1 cut(s) 498
Fnu4HI GCNGC 5 cut(s) 131, 549, 552, 561, 659
FokI GGATG 3 cut(s) 100, 464, 556
Fsp4HI GCNGC 5 cut(s) 131, 549, 552, 561, 659
FspBI CTAG 1 cut(s) 627
GlaI GCGC 1 cut(s) 500
GluI GCNGC 5 cut(s) 131, 549, 552, 561, 659
GsaI CCCAGC 1 cut(s) 752
HaeII RGCGCY 1 cut(s) 502
HaeIII GGCC 3 cut(s) 290, 578, 834
HapII CCGG 1 cut(s) 502
HgaI GACGC 1 cut(s) 578
HhaI GCGC 1 cut(s) 501
Hin1II CATG 4 cut(s) 160, 272, 583, 823
Hin6I GCGC 1 cut(s) 499
HinP1I GCGC 1 cut(s) 499
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 6 cut(s) 78, 679, 713, 724, 809, 872
HpaI GTTAAC 1 cut(s) 363
HpaII CCGG 1 cut(s) 502
HphI GGTGA 1 cut(s) 797
Hpy166II GTNNAC 2 cut(s) 363, 854
Hpy188I TCNGA 5 cut(s) 307, 597, 718, 778, 877
Hpy188III TCNNGA 3 cut(s) 53, 269, 532
Hpy8I GTNNAC 2 cut(s) 363, 854
HpyAV CCTTC 3 cut(s) 389, 529, 776
HpyCH4III ACNGT 2 cut(s) 119, 336
HpyCH4IV ACGT 1 cut(s) 520
HpyCH4V TGCA 9 cut(s) 4, 347, 389, 524, 554, 560, 691, 765, 854
HpyF10VI GCNNNNNNNGC 9 cut(s) 258, 287, 386, 557, 560, 569, 658, 745, 754
HpyF3I CTNAG 5 cut(s) 564, 648, 717, 743, 777
HpySE526I ACGT 1 cut(s) 520
Hsp92II CATG 4 cut(s) 160, 272, 583, 823
HspAI GCGC 1 cut(s) 499
KroI GCCGGC 1 cut(s) 501
KroNI GCCGGC 1 cut(s) 503
KspAI GTTAAC 1 cut(s) 363
Kzo9I GATC 2 cut(s) 582, 787
LguI GCTCTTC 1 cut(s) 729
Lsp1109I GCAGC 4 cut(s) 535, 538, 572, 670
LweI GCATC 2 cut(s) 376, 563
MaeI CTAG 1 cut(s) 627
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 2 cut(s) 209, 230
MalI GATC 2 cut(s) 584, 789
MboI GATC 2 cut(s) 582, 787
MboII GAAGA 8 cut(s) 288, 337, 395, 584, 616, 746, 836, 851
MfeI CAATTG 2 cut(s) 390, 728
MhlI GDGCHC 1 cut(s) 856
MlsI TGGCCA 1 cut(s) 578
MluCI AATT 9 cut(s) 67, 200, 256, 328, 369, 390, 433, 482, 728
MluNI TGGCCA 1 cut(s) 578
MlyI GAGTC 1 cut(s) 733
MnlI CCTC 8 cut(s) 406, 442, 471, 508, 559, 714, 772, 845
Mox20I TGGCCA 1 cut(s) 578
MreI CGCCGGCG 1 cut(s) 501
MroNI GCCGGC 1 cut(s) 501
MscI TGGCCA 1 cut(s) 578
MseI TTAA 2 cut(s) 198, 362
MslI CAYNNNNRTG 1 cut(s) 818
Msp20I TGGCCA 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 1 cut(s) 502
MspR9I CCNGG 1 cut(s) 623
MunI CAATTG 2 cut(s) 390, 728
MvaI CCWGG 1 cut(s) 623
MwoI GCNNNNNNNGC 9 cut(s) 258, 287, 386, 557, 560, 569, 658, 745, 754
NaeI GCCGGC 1 cut(s) 503
NdeII GATC 2 cut(s) 582, 787
NgoMIV GCCGGC 1 cut(s) 501
NlaIII CATG 4 cut(s) 160, 272, 583, 823
NmuCI GTSAC 2 cut(s) 209, 230
PaeR7I CTCGAG 1 cut(s) 530
PagI TCATGA 1 cut(s) 268
PciSI GCTCTTC 1 cut(s) 729
PdiI GCCGGC 1 cut(s) 503
PfeI GAWTC 5 cut(s) 78, 679, 713, 809, 872
PkrI GCNGC 5 cut(s) 132, 550, 553, 562, 660
PleI GAGTC 1 cut(s) 732
PmaCI CACGTG 1 cut(s) 521
PmlI CACGTG 1 cut(s) 521
PpsI GAGTC 1 cut(s) 732
Ppu21I YACGTR 1 cut(s) 521
PsiI TTATAA 1 cut(s) 701
Psp6I CCWGG 1 cut(s) 621
PspCI CACGTG 1 cut(s) 521
PspFI CCCAGC 1 cut(s) 748
PspGI CCWGG 1 cut(s) 621
PstI CTGCAG 1 cut(s) 562
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 818
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 2 cut(s) 198, 362
SatI GCNGC 5 cut(s) 131, 549, 552, 561, 659
Sau3AI GATC 2 cut(s) 582, 787
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 1 cut(s) 623
SduI GDGCHC 1 cut(s) 856
SetI ASST 6 cut(s) 523, 565, 649, 787, 804, 866
SfaNI GCATC 2 cut(s) 376, 563
SfcI CTRYAG 1 cut(s) 558
Sfr274I CTCGAG 1 cut(s) 530
SgrAI CRCCGGYG 1 cut(s) 501
SlaI CTCGAG 1 cut(s) 530
SmiMI CAYNNNNRTG 1 cut(s) 818
SmlI CTYRAG 2 cut(s) 53, 530
SmoI CTYRAG 2 cut(s) 53, 530
Sse9I AATT 9 cut(s) 67, 200, 256, 328, 369, 390, 433, 482, 728
SsiI CCGC 3 cut(s) 131, 261, 505
SspMI CTAG 1 cut(s) 627
StyD4I CCNGG 1 cut(s) 621
TaaI ACNGT 2 cut(s) 119, 336
TaiI ACGT 1 cut(s) 523
TaqI TCGA 3 cut(s) 531, 807, 837
TasI AATT 9 cut(s) 67, 200, 256, 328, 369, 390, 433, 482, 728
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 5 cut(s) 78, 679, 713, 809, 872
Tru1I TTAA 2 cut(s) 198, 362
Tru9I TTAA 2 cut(s) 198, 362
TscAI CASTG 4 cut(s) 235, 318, 681, 698
TseFI GTSAC 2 cut(s) 209, 230
TseI GCWGC 4 cut(s) 548, 551, 560, 658
Tsp45I GTSAC 2 cut(s) 209, 230
TspDTI ATGAA 4 cut(s) 285, 468, 684, 719
TspRI CASTG 4 cut(s) 235, 318, 681, 698
VneI GTGCAC 1 cut(s) 852
XapI RAATTY 4 cut(s) 67, 200, 328, 433
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.