pycom10g04250

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0018278
Physical Location & Seq
Reverse (-)
4481914 .. 4482762
849 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04250.1

Sequence Viewer

Length: 849 bp
ATGGAGTGTGCTTCATGTATCAAGTTTTTGAATCAGGGAAGTGATTTTGGATGTGGGTTTTTCATGTTTGGCCGTTTCTTGCCAGTTTTCAACTTGTTGGGGTTGTTTTTGGTGTTTATTTTGGGGCTAAAGGTTTTGCAAGTTGGGGTTCATTCGGAGATTTTTATGGAATTTTTATGTGATTTTAGAGGTAAATCAAGAGATTTCAGAAATCGGGTTTGTTCGACGTGTGGTTCTGGTGATGATGTCTGTTCTCCAAAGGTGAAATCATTGGAAACTTCACCAAATGCAGATTGCTTGTTGTTAAGCAAAAATGTCAATGCAGAAGAGAGAGATCATGTCGATATTAACGACGGTCATGGGGATAGCGATGGTGATGATGAAAGTGTTGGTTACGGCGAGGATGGGGAATTTGATGTGTTTGCATTGAGAAAAATGGTGAAGCTTGAGCGGCGAAGGAGGAATGAGGCTCGCGTAGAGCTCGAGAAGGAAAGGATGGCTGCCGCATCTGCAGCTGAGGAAGCTATGGCCATGATCCTGCGTCTTCAAAACGAGAAAAGCTGTATTGAAATCCAAGCCAAGCAGTACCGTCAAATGGCGGAGCAGAAGCAGCAGTACGATGAAGTGATGTTCCAATCGATGCAGTGGATTATAATGAAACACGAATCGGAGAGGAGTCTACTGGAGGAGCAACTGAGGCTGTACAGGCAAAAGTTGCAGCAATGTCTTAAGGGTGATGAAACGGTGGCACAATCCCAAGAAGCTGATCCGAGTTCCAACCTAATCGAGAATTGCATGGATGATCGGCTTGACGTAGATCCGTGGGAGCCGGTGCGACCGCAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

283

Amino Acids

32.33

Weight (kDa)

4.84

Isoelectric Point (pI)

56.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 142 - 232 8.9e-29 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18265
fragaria_vesca FvH4_2g11370
malus_domestica MD10G1055500.v1.1
prunus_persica Prupe.8G073500_v2.0.a1
pyrus_communis pycom10g04250
rosa_chinensis RchiOBHm_Chr6g0270031
rosa_roxburghii Rroxscaffold_7G00198190
rosa_rugosa Rorug06G0052000
rosa_samantha Rh6AG171100
rosa_wichuraiana Rw6G014710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 653
AccBSI CCGCTC 1 cut(s) 451
AccI GTMKAC 1 cut(s) 679
AccII CGCG 1 cut(s) 474
AciI CCGC 4 cut(s) 451, 504, 599, 839
AclWI GGATC 3 cut(s) 529, 761, 812
AcoI YGGCCR 2 cut(s) 70, 528
AcsI RAATTY 2 cut(s) 170, 410
AfaI GTAC 3 cut(s) 587, 617, 704
AfiI CCNNNNNNNGG 1 cut(s) 595
AflII CTTAAG 1 cut(s) 728
AflIII ACRYGT 1 cut(s) 227
AgsI TTSAA 4 cut(s) 31, 91, 548, 569
AjiI CACGTC 1 cut(s) 228
AluBI AGCT 6 cut(s) 445, 481, 515, 524, 561, 764
AluI AGCT 6 cut(s) 445, 481, 515, 524, 561, 764
Alw21I GWGCWC 1 cut(s) 483
AlwI GGATC 3 cut(s) 529, 761, 812
Ama87I CYCGRG 1 cut(s) 482
AoxI GGCC 2 cut(s) 70, 528
ApeKI GCWGC 4 cut(s) 500, 512, 610, 718
ApoI RAATTY 2 cut(s) 170, 410
AsuHPI GGTGA 6 cut(s) 251, 273, 274, 386, 451, 746
AvaI CYCGRG 1 cut(s) 482
BalI TGGCCA 1 cut(s) 530
BanII GRGCYC 1 cut(s) 483
BarI GAAGNNNNNNTAC 2 cut(s) 599, 631
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 483
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 4 cut(s) 487, 524, 622, 730
BccI CCATC 3 cut(s) 365, 398, 490
BceAI ACGGC 2 cut(s) 57, 412
BfmI CTRYAG 1 cut(s) 510
BfrI CTTAAG 1 cut(s) 728
BisI GCNGC 6 cut(s) 452, 501, 504, 513, 611, 719
BlsI GCNGC 6 cut(s) 453, 502, 505, 514, 612, 720
BmeT110I CYCGRG 1 cut(s) 482
BmgBI CACGTC 1 cut(s) 228
BmiI GGNNCC 1 cut(s) 828
BmsI GCATC 2 cut(s) 515, 630
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 704
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 467
Bsa29I ATCGAT 1 cut(s) 638
BsaJI CCNNGG 1 cut(s) 821
Bsc4I CCNNNNNNNGG 1 cut(s) 595
Bse118I RCCGGY 1 cut(s) 829
Bse1I ACTGG 2 cut(s) 83, 687
Bse3DI GCAATG 1 cut(s) 728
BseCI ATCGAT 1 cut(s) 638
BseDI CCNNGG 1 cut(s) 821
BseGI GGATG 4 cut(s) 56, 409, 501, 805
BseLI CCNNNNNNNGG 1 cut(s) 595
BseMI GCAATG 1 cut(s) 728
BseMII CTCAG 2 cut(s) 507, 686
BseNI ACTGG 2 cut(s) 83, 687
BseRI GAGGAG 2 cut(s) 688, 701
BseXI GCAGC 4 cut(s) 487, 524, 622, 730
Bsh1236I CGCG 1 cut(s) 474
Bsh1285I CGRYCG 1 cut(s) 839
BshFI GGCC 2 cut(s) 72, 530
BshVI ATCGAT 1 cut(s) 638
BsiEI CGRYCG 1 cut(s) 839
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 1 cut(s) 482
BsiSI CCGG 1 cut(s) 830
BslI CCNNNNNNNGG 1 cut(s) 595
BsnI GGCC 2 cut(s) 72, 530
BsoBI CYCGRG 1 cut(s) 482
Bsp1286I GDGCHC 1 cut(s) 483
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 5 cut(s) 334, 534, 766, 802, 817
BspACI CCGC 4 cut(s) 451, 504, 599, 839
BspANI GGCC 2 cut(s) 72, 530
BspCNI CTCAG 2 cut(s) 508, 687
BspDI ATCGAT 1 cut(s) 638
BspFNI CGCG 1 cut(s) 474
BspLI GGNNCC 1 cut(s) 828
BspMAI CTGCAG 1 cut(s) 514
BspPI GGATC 3 cut(s) 529, 761, 812
BspTI CTTAAG 1 cut(s) 728
BsrBI CCGCTC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 728
BsrFI RCCGGY 1 cut(s) 829
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 2 cut(s) 83, 687
BssAI RCCGGY 1 cut(s) 829
BssECI CCNNGG 1 cut(s) 821
BssMI GATC 5 cut(s) 334, 534, 766, 802, 817
Bst4CI ACNGT 3 cut(s) 356, 590, 745
Bst6I CTCTTC 1 cut(s) 321
BstAFI CTTAAG 1 cut(s) 728
BstAPI GCANNNNNTGC 1 cut(s) 715
BstAUI TGTACA 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 472
BstDEI CTNAG 2 cut(s) 516, 695
BstDSI CCRYGG 1 cut(s) 821
BstF5I GGATG 4 cut(s) 56, 409, 501, 805
BstFNI CGCG 1 cut(s) 474
BstKTI GATC 5 cut(s) 337, 537, 769, 805, 820
BstMBI GATC 5 cut(s) 334, 534, 766, 802, 817
BstMCI CGRYCG 1 cut(s) 839
BstMWI GCNNNNNNNGC 8 cut(s) 451, 509, 512, 521, 610, 697, 706, 715
BstSFI CTRYAG 1 cut(s) 510
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 4 cut(s) 487, 524, 622, 730
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 1 cut(s) 817
BstYI RGATCY 1 cut(s) 817
Bsu15I ATCGAT 1 cut(s) 638
BsuRI GGCC 2 cut(s) 72, 530
BsuTUI ATCGAT 1 cut(s) 638
BtgI CCRYGG 1 cut(s) 821
BtgZI GCGATG 1 cut(s) 384
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 4 cut(s) 56, 409, 501, 805
BtsI GCAGTG 1 cut(s) 650
BtsIMutI CAGTG 1 cut(s) 650
Cac8I GCNNGC 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 829
ClaI ATCGAT 1 cut(s) 638
CseI GACGC 1 cut(s) 530
Csp6I GTAC 3 cut(s) 586, 616, 703
CviAII CATG 6 cut(s) 15, 64, 338, 359, 532, 796
CviQI GTAC 3 cut(s) 586, 616, 703
DdeI CTNAG 2 cut(s) 516, 695
DpnI GATC 5 cut(s) 336, 536, 768, 804, 819
DpnII GATC 5 cut(s) 334, 534, 766, 802, 817
EaeI YGGCCR 2 cut(s) 70, 528
Eam1104I CTCTTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 321
EciI GGCGGA 1 cut(s) 614
Ecl136II GAGCTC 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 483
Eco53kI GAGCTC 1 cut(s) 481
Eco88I CYCGRG 1 cut(s) 482
EcoICRI GAGCTC 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 483
FaeI CATG 6 cut(s) 18, 67, 341, 362, 535, 799
FatI CATG 6 cut(s) 14, 63, 337, 358, 531, 795
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 6 cut(s) 452, 501, 504, 513, 611, 719
FokI GGATG 4 cut(s) 63, 416, 508, 812
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 6 cut(s) 452, 501, 504, 513, 611, 719
GluI GCNGC 6 cut(s) 452, 501, 504, 513, 611, 719
GsuI CTGGAG 1 cut(s) 704
HaeIII GGCC 2 cut(s) 72, 530
HapII CCGG 1 cut(s) 830
HgaI GACGC 1 cut(s) 530
Hin1II CATG 6 cut(s) 18, 67, 341, 362, 535, 799
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 3 cut(s) 31, 665, 676
HpaII CCGG 1 cut(s) 830
HphI GGTGA 6 cut(s) 251, 273, 274, 386, 451, 746
Hpy166II GTNNAC 1 cut(s) 680
Hpy188I TCNGA 4 cut(s) 157, 209, 670, 771
Hpy188III TCNNGA 3 cut(s) 198, 484, 787
Hpy8I GTNNAC 1 cut(s) 680
Hpy99I CGWCG 2 cut(s) 229, 356
HpyAV CCTTC 2 cut(s) 450, 481
HpyCH4III ACNGT 3 cut(s) 356, 590, 745
HpyCH4IV ACGT 2 cut(s) 227, 813
HpyCH4V TGCA 8 cut(s) 139, 290, 323, 425, 512, 643, 718, 795
HpyF10VI GCNNNNNNNGC 8 cut(s) 451, 509, 512, 521, 610, 697, 706, 715
HpyF3I CTNAG 2 cut(s) 516, 695
HpySE526I ACGT 2 cut(s) 227, 813
Hsp92II CATG 6 cut(s) 18, 67, 341, 362, 535, 799
Kzo9I GATC 5 cut(s) 334, 534, 766, 802, 817
LmnI GCTCC 3 cut(s) 601, 688, 826
LpnPI CCDG 7 cut(s) 20, 96, 222, 551, 668, 691, 843
Lsp1109I GCAGC 4 cut(s) 487, 524, 622, 730
LweI GCATC 2 cut(s) 515, 630
MaeII ACGT 2 cut(s) 227, 813
MaeIII GTNAC 1 cut(s) 392
MalI GATC 5 cut(s) 336, 536, 768, 804, 819
MbiI CCGCTC 1 cut(s) 451
MboI GATC 5 cut(s) 334, 534, 766, 802, 817
MboII GAAGA 2 cut(s) 338, 536
MflI RGATCY 1 cut(s) 817
MhlI GDGCHC 1 cut(s) 483
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 4 cut(s) 170, 410, 790, 842
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 1 cut(s) 685
MmeI TCCRAC 1 cut(s) 801
MnlI CCTC 8 cut(s) 182, 394, 453, 460, 511, 666, 679, 690
Mox20I TGGCCA 1 cut(s) 530
MscI TGGCCA 1 cut(s) 530
MseI TTAA 3 cut(s) 305, 348, 729
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 515
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 1 cut(s) 830
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 8 cut(s) 451, 509, 512, 521, 610, 697, 706, 715
NdeII GATC 5 cut(s) 334, 534, 766, 802, 817
NlaIII CATG 6 cut(s) 18, 67, 341, 362, 535, 799
NlaIV GGNNCC 1 cut(s) 828
PaeR7I CTCGAG 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PfeI GAWTC 2 cut(s) 31, 665
PkrI GCNGC 6 cut(s) 453, 502, 505, 514, 612, 720
PleI GAGTC 1 cut(s) 684
PpsI GAGTC 1 cut(s) 684
PsiI TTATAA 1 cut(s) 653
Psp124BI GAGCTC 1 cut(s) 483
PspN4I GGNNCC 1 cut(s) 828
PstI CTGCAG 1 cut(s) 514
PsuI RGATCY 1 cut(s) 817
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 3 cut(s) 587, 617, 704
RsaNI GTAC 3 cut(s) 586, 616, 703
SacI GAGCTC 1 cut(s) 483
SaqAI TTAA 3 cut(s) 305, 348, 729
SatI GCNGC 6 cut(s) 452, 501, 504, 513, 611, 719
Sau3AI GATC 5 cut(s) 334, 534, 766, 802, 817
SchI GAGTC 1 cut(s) 685
SduI GDGCHC 1 cut(s) 483
SfaNI GCATC 2 cut(s) 515, 630
SfcI CTRYAG 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 482
SlaI CTCGAG 1 cut(s) 482
SmlI CTYRAG 3 cut(s) 446, 482, 728
SmoI CTYRAG 3 cut(s) 446, 482, 728
Sse9I AATT 4 cut(s) 170, 410, 790, 842
SsiI CCGC 4 cut(s) 451, 504, 599, 839
SstI GAGCTC 1 cut(s) 483
TaaI ACNGT 3 cut(s) 356, 590, 745
TaiI ACGT 2 cut(s) 230, 816
TaqI TCGA 5 cut(s) 224, 342, 483, 638, 786
TasI AATT 4 cut(s) 170, 410, 790, 842
TatI WGTACW 1 cut(s) 702
TauI GCSGC 2 cut(s) 454, 506
TfiI GAWTC 2 cut(s) 31, 665
Tru1I TTAA 3 cut(s) 305, 348, 729
Tru9I TTAA 3 cut(s) 305, 348, 729
TscAI CASTG 1 cut(s) 650
TseI GCWGC 4 cut(s) 500, 512, 610, 718
TspDTI ATGAA 6 cut(s) 52, 140, 396, 636, 671, 753
TspGWI ACGGA 1 cut(s) 810
TspRI CASTG 1 cut(s) 650
Vha464I CTTAAG 1 cut(s) 728
XapI RAATTY 2 cut(s) 170, 410
XhoI CTCGAG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.