FvH4_2g14810

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015541
Physical Location & Seq
Forward (+)
13017046 .. 13018854
1809 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14810.t1

Sequence Viewer

Length: 1809 bp
ATGCATCAAAAATTGCTGAAAATCTTACATAACTGCAACTCTCTGAAACAACTGAAACAGACCCACCTTCACATCTTCATTCATGGGCTCCAAGATAGCACCTTCTTACTCCCCAAGCTCCTCACTCTCTCCTCACAACTGGGTTCTCCTCATTACGCTCATTCCATTTTCCAATACTCTCGCTGCCCCAATGTTGTGGCTTGCAACACAATGATACAATGCTTCATTGGAAAGACCCCAGTAGGCGCATTGCAAGTCTACGATCGAATGAAAGCATTGAAAGTTTCGCCGAATAGTTATACTTTCACTTTTCTTCTTAGCTGCTTTGAATCCTGTGAGGCCCTTGAAGATGGGAGGGTGGTTCATGGTGACATTGTCAAACTTGGTTTCAGCTCCAGCGTCTTTGTTCAGAACACCCTTTTGGATTTCTATGCGAAATGTGCCGGCGGTTTTGGTTTTGATTCTGCACGGAAGGTGTTTGATGAAATGCCTGAGAGGGATGTGGTTTCTTGGAATTCTATGATTGCTAGTTACATGAGTCGTGGTGGGGTAGAGCCTGCAATGAGGCTGTTTGAGTCAGTTCCGGAGAAAAACATTGTGACATGGAACTCTGTTGTTTCTGGTCTTTCCAAGGCCGGGAACATGGAGTTGGCCCGTTCGGTTTTTGAGAAGATGCCTGAAAGAAATGATGTTTCCTGGAATTCATTGATAACTGCGTATATACGGTTGGGTGATATGAGCTCAGCTCGGTGTTTATTTGAACAAATGCCTGAGAAAACAGTTGTTTCTTGGACCGCCATGGTGTCTGGATACAACATCATTGGAGATCTTGAATCAGCCAGGAATGTATTCAGTTTGATGCCGGTGAAAAATGTTGTATCGTGGAATGCTATGATTGCAGGTTATGTTCACAACCACATGTTTGATCAAGCTCTTTCTGTATTCCGCAAGATGTTGATTGATGGCAAGTGCAGGCCTGATCAGAGCACTATGATCAGTGTGCTATCGGCATGTACTCACTTGGGTTCTCTTGAACATGGGAAATGGACCGAGTCTTATATTAAGAGGAACAAGTTTGACATGTCTGTACCTTTGGGAAATGCTCTAATAGACATGTTCGCAAAGTGTGGAGTTGTAGAAAATGCAAAAGCTGTTTTTGATAAGATGGACAACAGATGTATAATTACATGGACGACAATGGTTTCAGGTCTAGCTGTTAATGGGTGGTGCAGAGAAGCATTAGCTCTCTTTGAAACAATGTGTTTAAGAGGACCTAAACCAGATGATGTGATTTTTATTGCTGTTCTATCAGCTTGCACTCATGGAGGGCTGGTAGACGAGGGGAAACAAATTTTTTATCAGATGGAACATGAGTTTGACATCAAGCCCCGAATAGAGCATTATGGGTGCATGGTTGATCTTCTAGGTCGAGCCGGGAAGTTGGAAGAAGCACTCAGATTTGTAGAAAACATGCATTTGGAGCCAAATGCTGTTATTTGGGCTACTCTACTGGGTTCTTGTAAGATTCATGGAAGTGGAGATCTGGTGGAGTCTGTAACCAGGAGGATTATAGAGCAGGAACCGGAAAATCCCAGTTATTTAACTCTAGTTTCTAATTTGAGTGCATCAGTTGGACAATGGAAAGATGTGCTGACCTATCGGCAAGTTATGAGACAACAGGGAATAGAAAAGGTTCCTGGTTGTAGCTCAATCCAAATGGGGAACAAGATAGATGAGTTTCTTGCCCACGATACAAGGCATGACGAAAGAAAGAAAAGAGATTTATGGAGTTTTGAATTGTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

603

Amino Acids

67.77

Weight (kDa)

6.68

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 63 - 109 4.1e-09 PPR repeat family
PPR_2 PF13041 197 - 229 2.1e-06 PPR repeat family
PPR PF01535 200 - 229 1.6e-07 PPR repeat
PPR PF01535 231 - 259 3.3e-06 PPR repeat
PPR_2 PF13041 290 - 338 6.1e-09 PPR repeat family
PPR PF01535 293 - 321 1.2e-06 PPR repeat
PPR PF01535 395 - 424 6.9e-06 PPR repeat
E_motif PF20431 516 - 572 1.3e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015541)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g14810
malus_domestica MD14G1026500.v1.1
prunus_persica Prupe.8G017600_v2.0.a1
pyrus_communis pycom14g02530
rosa_chinensis RchiOBHm_Chr6g0277401
rosa_laevigata RLG00000013305
rosa_multiflora Rmu_co8455877.1_g000001 Rmu_sc0000030.1_g000006 Rmu_sc0004888.1_g000004
rosa_roxburghii Rroxscaffold_7G00191380
rosa_rugosa Rorug06G0107800 Rorug06G0107800 Rorug06G0107800 Rorug06G0107900
rosa_samantha Rh6DG215400
rosa_wichuraiana Rw6G019160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 890
AccI GTMKAC 2 cut(s) 258, 1335
AccIII TCCGGA 1 cut(s) 583
AciI CCGC 3 cut(s) 447, 795, 946
AcsI RAATTY 3 cut(s) 514, 700, 1350
AfaI GTAC 2 cut(s) 1015, 1089
AfiI CCNNNNNNNGG 1 cut(s) 636
AflIII ACRYGT 3 cut(s) 918, 1080, 1113
AgsI TTSAA 9 cut(s) 280, 329, 347, 761, 833, 1034, 1253, 1796, 1805
AjnI CCWGG 4 cut(s) 695, 839, 1559, 1696
AjuI GAANNNNNNNTTGG 4 cut(s) 404, 436, 632, 664
Alw21I GWGCWC 2 cut(s) 743, 989
Alw26I GTCTC 1 cut(s) 1666
Aor13HI TCCGGA 1 cut(s) 583
AoxI GGCC 4 cut(s) 339, 633, 651, 974
ApeKI GCWGC 2 cut(s) 183, 321
ApoI RAATTY 3 cut(s) 514, 700, 1350
Asp700I GAANNNNTTC 2 cut(s) 848, 1692
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 5 cut(s) 340, 652, 792, 1047, 1271
AsuC2I CCSGG 2 cut(s) 637, 1435
AsuHPI GGTGA 3 cut(s) 380, 743, 877
AvaII GGWCC 3 cut(s) 792, 1047, 1271
BanII GRGCYC 2 cut(s) 90, 743
Bbv12I GWGCWC 2 cut(s) 743, 989
BbvI GCAGC 2 cut(s) 170, 308
BccI CCATC 4 cut(s) 344, 956, 1159, 1357
BciT130I CCWGG 4 cut(s) 697, 841, 1561, 1698
BciVI GTATCC 1 cut(s) 803
BclI TGATCA 3 cut(s) 925, 979, 993
BcnI CCSGG 2 cut(s) 637, 1435
BcoDI GTCTC 1 cut(s) 1666
BfaI CTAG 4 cut(s) 528, 1211, 1424, 1607
BfuAI ACCTGC 1 cut(s) 890
BfuI GTATCC 1 cut(s) 803
BglII AGATCT 2 cut(s) 826, 1540
BisI GCNGC 2 cut(s) 184, 322
BlpI GCTNAGC 1 cut(s) 742
BlsI GCNGC 2 cut(s) 185, 323
Bme1390I CCNGG 6 cut(s) 637, 697, 841, 1435, 1561, 1698
Bme18I GGWCC 3 cut(s) 792, 1047, 1271
BmgT120I GGNCC 5 cut(s) 340, 652, 792, 1047, 1271
BmiI GGNNCC 4 cut(s) 89, 1482, 1581, 1695
BmrFI CCNGG 6 cut(s) 637, 697, 841, 1435, 1561, 1698
BmrI ACTGGG 4 cut(s) 149, 233, 1520, 1587
BmsI GCATC 4 cut(s) 13, 663, 849, 1634
BmuI ACTGGG 4 cut(s) 149, 233, 1520, 1587
BplI GAGNNNNNCTC 2 cut(s) 730, 762
BpmI CTGGAG 1 cut(s) 379
Bpu1102I GCTNAGC 1 cut(s) 742
BpuMI CCSGG 2 cut(s) 637, 1435
BsaJI CCNNGG 2 cut(s) 630, 798
BsaWI WCCGGW 2 cut(s) 583, 1582
BsaXI ACNNNNNCTCC 4 cut(s) 1317, 1347, 1530, 1560
Bsc4I CCNNNNNNNGG 1 cut(s) 636
Bse118I RCCGGY 2 cut(s) 443, 862
Bse1I ACTGG 4 cut(s) 144, 239, 1515, 1593
Bse3DI GCAATG 2 cut(s) 248, 567
BseAI TCCGGA 1 cut(s) 583
BseBI CCWGG 4 cut(s) 697, 841, 1561, 1698
BseDI CCNNGG 2 cut(s) 630, 798
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 1 cut(s) 636
BseMI GCAATG 2 cut(s) 248, 567
BseMII CTCAG 4 cut(s) 483, 756, 762, 1468
BseNI ACTGG 4 cut(s) 144, 239, 1515, 1593
BseRI GAGGAG 3 cut(s) 110, 121, 138
BseXI GCAGC 2 cut(s) 170, 308
BsgI GTGCAG 3 cut(s) 450, 991, 1249
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 4 cut(s) 341, 635, 653, 976
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 743, 989
BsiSI CCGG 6 cut(s) 444, 584, 636, 863, 1434, 1583
BslI CCNNNNNNNGG 1 cut(s) 636
BsmAI GTCTC 1 cut(s) 1666
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 4 cut(s) 341, 635, 653, 976
Bsp1286I GDGCHC 3 cut(s) 90, 743, 989
Bsp13I TCCGGA 1 cut(s) 583
Bsp143I GATC 7 cut(s) 262, 826, 925, 979, 993, 1417, 1540
Bsp1720I GCTNAGC 1 cut(s) 742
Bsp19I CCATGG 1 cut(s) 798
BspACI CCGC 3 cut(s) 447, 795, 946
BspANI GGCC 4 cut(s) 341, 635, 653, 976
BspCNI CTCAG 4 cut(s) 484, 755, 763, 1467
BspEI TCCGGA 1 cut(s) 583
BspLI GGNNCC 4 cut(s) 89, 1482, 1581, 1695
BspMI ACCTGC 1 cut(s) 890
BsrDI GCAATG 2 cut(s) 248, 567
BsrFI RCCGGY 2 cut(s) 443, 862
BsrI ACTGG 4 cut(s) 144, 239, 1515, 1593
BssAI RCCGGY 2 cut(s) 443, 862
BssECI CCNNGG 2 cut(s) 630, 798
BssMI GATC 7 cut(s) 262, 826, 925, 979, 993, 1417, 1540
BssT1I CCWWGG 2 cut(s) 630, 798
Bst2UI CCWGG 4 cut(s) 697, 841, 1561, 1698
Bst4CI ACNGT 2 cut(s) 726, 781
BstC8I GCNNGC 5 cut(s) 202, 445, 558, 974, 1315
BstDEI CTNAG 5 cut(s) 317, 492, 742, 771, 1454
BstDSI CCRYGG 1 cut(s) 798
BstF5I GGATG 1 cut(s) 505
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 7 cut(s) 265, 829, 928, 982, 996, 1420, 1543
BstMAI GTCTC 1 cut(s) 1666
BstMBI GATC 7 cut(s) 262, 826, 925, 979, 993, 1417, 1540
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 3 cut(s) 440, 896, 1480
BstNI CCWGG 4 cut(s) 697, 841, 1561, 1698
BstNSI RCATGY 5 cut(s) 922, 1014, 1084, 1117, 1474
BstSCI CCNGG 6 cut(s) 635, 695, 839, 1433, 1559, 1696
BstV1I GCAGC 2 cut(s) 170, 308
BstX2I RGATCY 2 cut(s) 826, 1540
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 2 cut(s) 826, 1540
BsuI GTATCC 1 cut(s) 803
BsuRI GGCC 4 cut(s) 341, 635, 653, 976
BtgI CCRYGG 1 cut(s) 798
BtsCI GGATG 1 cut(s) 505
BtsIMutI CAGTG 1 cut(s) 1003
BveI ACCTGC 1 cut(s) 890
Cac8I GCNNGC 5 cut(s) 202, 445, 558, 974, 1315
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 2 cut(s) 443, 862
Cfr13I GGNCC 5 cut(s) 340, 652, 792, 1047, 1271
CseI GACGC 1 cut(s) 388
Csp6I GTAC 2 cut(s) 1014, 1088
CviQI GTAC 2 cut(s) 1014, 1088
DdeI CTNAG 5 cut(s) 317, 492, 742, 771, 1454
DpnI GATC 7 cut(s) 264, 828, 927, 981, 995, 1419, 1542
DpnII GATC 7 cut(s) 262, 826, 925, 979, 993, 1417, 1540
Ecl136II GAGCTC 1 cut(s) 741
Eco130I CCWWGG 2 cut(s) 630, 798
Eco147I AGGCCT 1 cut(s) 976
Eco24I GRGCYC 2 cut(s) 90, 743
Eco47I GGWCC 3 cut(s) 792, 1047, 1271
Eco53kI GAGCTC 1 cut(s) 741
EcoICRI GAGCTC 1 cut(s) 741
EcoO109I RGGNCCY 2 cut(s) 340, 1271
EcoRI GAATTC 2 cut(s) 514, 700
EcoRII CCWGG 4 cut(s) 695, 839, 1559, 1696
EcoT14I CCWWGG 2 cut(s) 630, 798
EcoT22I ATGCAT 2 cut(s) 6, 1476
EcoT38I GRGCYC 2 cut(s) 90, 743
ErhI CCWWGG 2 cut(s) 630, 798
FbaI TGATCA 3 cut(s) 925, 979, 993
FblI GTMKAC 2 cut(s) 258, 1335
Fnu4HI GCNGC 2 cut(s) 184, 322
FokI GGATG 1 cut(s) 512
FriOI GRGCYC 2 cut(s) 90, 743
Fsp4HI GCNGC 2 cut(s) 184, 322
FspBI CTAG 4 cut(s) 528, 1211, 1424, 1607
GlaI GCGC 1 cut(s) 247
GluI GCNGC 2 cut(s) 184, 322
GsuI CTGGAG 1 cut(s) 379
HaeIII GGCC 4 cut(s) 341, 635, 653, 976
HapII CCGG 6 cut(s) 444, 584, 636, 863, 1434, 1583
HgaI GACGC 1 cut(s) 388
HhaI GCGC 1 cut(s) 248
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HinfI GANTC 8 cut(s) 329, 461, 538, 575, 833, 1052, 1525, 1550
HpaII CCGG 6 cut(s) 444, 584, 636, 863, 1434, 1583
HphI GGTGA 3 cut(s) 380, 743, 877
Hpy166II GTNNAC 3 cut(s) 259, 910, 1336
Hpy188I TCNGA 5 cut(s) 45, 411, 984, 1362, 1457
Hpy188III TCNNGA 4 cut(s) 584, 807, 830, 1031
Hpy8I GTNNAC 3 cut(s) 259, 910, 1336
HpyAV CCTTC 3 cut(s) 77, 112, 466
HpyCH4III ACNGT 2 cut(s) 726, 781
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 896, 1480
HpyF3I CTNAG 5 cut(s) 317, 492, 742, 771, 1454
HspAI GCGC 1 cut(s) 246
Kpn2I TCCGGA 1 cut(s) 583
KroI GCCGGC 1 cut(s) 443
KroNI GCCGGC 1 cut(s) 445
Ksp22I TGATCA 3 cut(s) 925, 979, 993
Kzo9I GATC 7 cut(s) 262, 826, 925, 979, 993, 1417, 1540
LmnI GCTCC 4 cut(s) 93, 123, 398, 1480
Lsp1109I GCAGC 2 cut(s) 170, 308
LweI GCATC 4 cut(s) 13, 663, 849, 1634
MaeI CTAG 4 cut(s) 528, 1211, 1424, 1607
MaeIII GTNAC 4 cut(s) 368, 530, 598, 1555
MalI GATC 7 cut(s) 264, 828, 927, 981, 995, 1419, 1542
MboI GATC 7 cut(s) 262, 826, 925, 979, 993, 1417, 1540
MboII GAAGA 6 cut(s) 67, 305, 359, 682, 1412, 1457
MflI RGATCY 2 cut(s) 826, 1540
MhlI GDGCHC 3 cut(s) 90, 743, 989
MluCI AATT 7 cut(s) 11, 514, 700, 1182, 1350, 1615, 1796
MlyI GAGTC 4 cut(s) 547, 584, 1061, 1559
MmeI TCCRAC 2 cut(s) 1422, 1612
Mph1103I ATGCAT 2 cut(s) 6, 1476
MroI TCCGGA 1 cut(s) 583
MroNI GCCGGC 1 cut(s) 443
MroXI GAANNNNTTC 2 cut(s) 848, 1692
MseI TTAA 4 cut(s) 1062, 1218, 1265, 1601
MslI CAYNNNNRTG 1 cut(s) 1533
MspI CCGG 6 cut(s) 444, 584, 636, 863, 1434, 1583
MspR9I CCNGG 6 cut(s) 637, 697, 841, 1435, 1561, 1698
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 4 cut(s) 697, 841, 1561, 1698
MwoI GCNNNNNNNGC 3 cut(s) 440, 896, 1480
NaeI GCCGGC 1 cut(s) 445
NciI CCSGG 2 cut(s) 637, 1435
NcoI CCATGG 1 cut(s) 798
NdeII GATC 7 cut(s) 262, 826, 925, 979, 993, 1417, 1540
NgoMIV GCCGGC 1 cut(s) 443
NlaIV GGNNCC 4 cut(s) 89, 1482, 1581, 1695
NmuCI GTSAC 2 cut(s) 368, 598
NsiI ATGCAT 2 cut(s) 6, 1476
NspI RCATGY 5 cut(s) 922, 1014, 1084, 1117, 1474
PceI AGGCCT 1 cut(s) 976
PciI ACATGT 3 cut(s) 918, 1080, 1113
PctI GAATGC 1 cut(s) 892
PdiI GCCGGC 1 cut(s) 445
PdmI GAANNNNTTC 2 cut(s) 848, 1692
PfeI GAWTC 4 cut(s) 329, 461, 833, 1525
PflFI GACNNNGTC 2 cut(s) 374, 1051
PfoI TCCNGGA 1 cut(s) 695
PkrI GCNGC 2 cut(s) 185, 323
Ple19I CGATCG 1 cut(s) 265
PleI GAGTC 4 cut(s) 546, 583, 1060, 1558
PpsI GAGTC 4 cut(s) 546, 583, 1060, 1558
PpuMI RGGWCCY 1 cut(s) 1271
PscI ACATGT 3 cut(s) 918, 1080, 1113
Psp124BI GAGCTC 1 cut(s) 743
Psp5II RGGWCCY 1 cut(s) 1271
Psp6I CCWGG 4 cut(s) 695, 839, 1559, 1696
PspGI CCWGG 4 cut(s) 695, 839, 1559, 1696
PspN4I GGNNCC 4 cut(s) 89, 1482, 1581, 1695
PspPI GGNCC 5 cut(s) 340, 652, 792, 1047, 1271
PspPPI RGGWCCY 1 cut(s) 1271
PsuI RGATCY 2 cut(s) 826, 1540
PsyI GACNNNGTC 2 cut(s) 374, 1051
PvuI CGATCG 1 cut(s) 265
RsaI GTAC 2 cut(s) 1015, 1089
RsaNI GTAC 2 cut(s) 1014, 1088
RseI CAYNNNNRTG 1 cut(s) 1533
SacI GAGCTC 1 cut(s) 743
SaqAI TTAA 4 cut(s) 1062, 1218, 1265, 1601
SatI GCNGC 2 cut(s) 184, 322
Sau3AI GATC 7 cut(s) 262, 826, 925, 979, 993, 1417, 1540
Sau96I GGNCC 5 cut(s) 340, 652, 792, 1047, 1271
SchI GAGTC 4 cut(s) 547, 584, 1061, 1559
ScrFI CCNGG 6 cut(s) 637, 697, 841, 1435, 1561, 1698
SduI GDGCHC 3 cut(s) 90, 743, 989
SfaNI GCATC 4 cut(s) 13, 663, 849, 1634
SinI GGWCC 3 cut(s) 792, 1047, 1271
SmiMI CAYNNNNRTG 1 cut(s) 1533
Sse9I AATT 7 cut(s) 11, 514, 700, 1182, 1350, 1615, 1796
SseBI AGGCCT 1 cut(s) 976
SsiI CCGC 3 cut(s) 447, 795, 946
SspMI CTAG 4 cut(s) 528, 1211, 1424, 1607
SstI GAGCTC 1 cut(s) 743
StuI AGGCCT 1 cut(s) 976
StyD4I CCNGG 6 cut(s) 635, 695, 839, 1433, 1559, 1696
StyI CCWWGG 2 cut(s) 630, 798
TaaI ACNGT 2 cut(s) 726, 781
TaqI TCGA 2 cut(s) 265, 1429
TaqII GACCGA 1 cut(s) 1064
TasI AATT 7 cut(s) 11, 514, 700, 1182, 1350, 1615, 1796
TatI WGTACW 1 cut(s) 1013
TfiI GAWTC 4 cut(s) 329, 461, 833, 1525
Tru1I TTAA 4 cut(s) 1062, 1218, 1265, 1601
Tru9I TTAA 4 cut(s) 1062, 1218, 1265, 1601
TscAI CASTG 1 cut(s) 1003
TseFI GTSAC 2 cut(s) 368, 598
TseI GCWGC 2 cut(s) 183, 321
Tsp45I GTSAC 2 cut(s) 368, 598
TspDTI ATGAA 8 cut(s) 67, 71, 214, 284, 353, 498, 693, 1517
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 1 cut(s) 1003
Tth111I GACNNNGTC 2 cut(s) 374, 1051
VpaK11BI GGWCC 3 cut(s) 792, 1047, 1271
XapI RAATTY 3 cut(s) 514, 700, 1350
XceI RCATGY 5 cut(s) 922, 1014, 1084, 1117, 1474
XmiI GTMKAC 2 cut(s) 258, 1335
XmnI GAANNNNTTC 2 cut(s) 848, 1692
XspI CTAG 4 cut(s) 528, 1211, 1424, 1607
Zsp2I ATGCAT 2 cut(s) 6, 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.