FvH4_2g15670

Serine threonine protein phosphatase 2A 57 kDa regulatory subunit B' beta isoform-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015544
Physical Location & Seq
Reverse (-)
13749656 .. 13751612
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15670.t1

Sequence Viewer

Length: 1404 bp
ATGGGTGTGCAAAGTAACACTTCCCTGAAAGCTTCACCTAGAGTTTCGACGAGGAAAAGATCAACAACTCTTCAACACCTCTTTGATCTTGATTCCAATGGCAACAAGATTGCCAACAACAATATAGGGAGTTCATCGTCATCTTCTTCATCTTCTTTCAACTCGGAGCACGAAGAAATTCTTTCCACAGTGTCCTACTGCACTTTTGTTTTCGCCTTCACTGAATACGAATGTCCTACACAACAGGAACTCAAGAGGCATAAGCTCTTGCAGCTTCTTTCCTTTGTGAAGACTAATAAAAATTCAGTTCCTGTTCAAGTTCTGTCGTCTCTCATTTCCATGATATCAGCCAACTTGTTCAGGCCTCTCCCTCCTCCTTCCAATCCCTCCATCACCGCAGACTTTCCAGATGATGAAGACCCCATGTCAGCCTTTTCTCCAGTTTGGTCACACCTACAGTTAGTTTATGACATCCTCATACGAGTAGTGATCAACAACGATCCCAAGGTACTTCGTCAGTACATTGATAACCAGTTTGTCATCAATCTCATCGCTCTTTTCCAATCCGAAGACCCTAGGGAACGTGACAGCTTGAAGAATGTGTACCACCGTATATATTCCAGATTCACATTCTACCGCGCCTTCATGAGGAAGTCCATGAATGATGTGTTCTTGCACTATGTGTTTGAGACAGAGAGGCATTGTGGGATTAGAGAGTTGCTTGAGATATGGGGAAGCATCATCAATGGCTTCACCGTGCCGCTTAAGGAAGAGCACAAGCTGTTCTTGATGAGGGTGCTCATTCCATTTCACAAGACAAAAGGAATGCAGGCTTATCATAGGCAATTGGCTTATTGTGTGTCTCAGTTTGTCCAGAAGGAGCCAGTGCTAGGTGGAATTGTTGTTAGAGGGATTTTGAGGTATTGGCCTGTCACTAATTGCCAGAAGGAGGTTTTGCTGATAGGGGAACTTGAAGAACTTGTCGAAAATATTGATCCTGATCAGTATAGGAAGCTAGCTTTGCCTATATGTAATCAGATTACAAGATGCTTGAACAGTTGGAACTCACAGGTAGCGGAGCGAGCGCTATATGTCTGGAACAATGAGCATTTTGTGAAAATGATATCAGTAGCAATGGAGGAAGTGTTTCCAGTAGTAGTTGAAGGCATGGAGAAGAACTTGAAGTGGCATTGGAGCAAAAGCGTGCGACAATTGACAGAAAACGTGAAAGTGATGCTCGAAGAAATGGACCCTATTTTGTACTCGAAATGTCTGCAAGAAATTGAGCTCAGAGGATCCAAGGCTCACCAGGCTGAGATCAAGAGGAAGCAGAATTGGGACAGAATAGAAATGGCAGCAGCTCGGCACCAGTATCTCCAACCACCACATTTCATATGTGTCTAA

Protein Analysis

468

Amino Acids

54.25

Weight (kDa)

8.26

Isoelectric Point (pI)

52.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 52 - 453 9.9e-143 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015544)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g15670
malus_domestica MD05G1125100.v1.1
prunus_persica Prupe.8G169200_v2.0.a1
pyrus_communis pycom05g11940 pycom10g10970
rosa_chinensis RchiOBHm_Chr6g0278971
rosa_laevigata RLG00000013179
rosa_multiflora Rmu_sc0002080.1_g000042 Rmu_sc0009618.1_g000015
rosa_roxburghii Rroxscaffold_7G00189300
rosa_rugosa Rorug06G0120500
rosa_samantha Rh6AG229200 Rh6BG234400 Rh6CG236600 Rh6DG228200
rosa_wichuraiana Rw6G020070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1365
AccII CGCG 1 cut(s) 639
AciI CCGC 4 cut(s) 396, 637, 761, 1076
AclWI GGATC 4 cut(s) 494, 989, 1290, 1303
AcsI RAATTY 2 cut(s) 177, 301
AdeI CACNNNGTG 1 cut(s) 682
AfaI GTAC 4 cut(s) 510, 521, 605, 1262
AfeI AGCGCT 1 cut(s) 1086
AfiI CCNNNNNNNGG 3 cut(s) 648, 890, 949
AflII CTTAAG 1 cut(s) 764
AgsI TTSAA 8 cut(s) 74, 160, 317, 595, 974, 1054, 1163, 1183
AhdI GACNNNNNGTC 1 cut(s) 424
AjnI CCWGG 1 cut(s) 1308
AluBI AGCT 9 cut(s) 32, 265, 274, 591, 781, 1015, 1019, 1288, 1361
AluI AGCT 9 cut(s) 32, 265, 274, 591, 781, 1015, 1019, 1288, 1361
Alw21I GWGCWC 4 cut(s) 171, 777, 801, 1290
Alw26I GTCTC 3 cut(s) 333, 683, 867
AlwI GGATC 4 cut(s) 494, 989, 1290, 1303
AlwNI CAGNNNCTG 1 cut(s) 311
Aor51HI AGCGCT 1 cut(s) 1086
AoxI GGCC 2 cut(s) 362, 926
ApeKI GCWGC 3 cut(s) 271, 1355, 1358
ApoI RAATTY 2 cut(s) 177, 301
Asp700I GAANNNNTTC 2 cut(s) 177, 1146
AspA2I CCTAGG 1 cut(s) 575
AspLEI GCGC 2 cut(s) 641, 1087
AspS9I GGNCC 1 cut(s) 1249
AsuHPI GGTGA 4 cut(s) 27, 385, 745, 1298
AsuNHI GCTAGC 1 cut(s) 1015
AvaII GGWCC 1 cut(s) 1249
AvrII CCTAGG 1 cut(s) 575
BamHI GGATCC 1 cut(s) 1295
BanI GGYRCC 1 cut(s) 1365
BanII GRGCYC 1 cut(s) 1290
BarI GAAGNNNNNNTAC 2 cut(s) 587, 619
BbsI GAAGAC 3 cut(s) 296, 423, 576
Bbv12I GWGCWC 4 cut(s) 171, 777, 801, 1290
BbvI GCAGC 3 cut(s) 283, 1367, 1370
BccI CCATC 1 cut(s) 398
BcgI CGANNNNNNTGC 2 cut(s) 1255, 1289
BciT130I CCWGG 1 cut(s) 1310
BclI TGATCA 2 cut(s) 489, 1000
BcoDI GTCTC 3 cut(s) 333, 683, 867
BfaI CTAG 4 cut(s) 39, 576, 890, 1016
BfmI CTRYAG 1 cut(s) 455
BfoI RGCGCY 1 cut(s) 1088
BfrI CTTAAG 1 cut(s) 764
BisI GCNGC 4 cut(s) 272, 761, 1356, 1359
BlnI CCTAGG 1 cut(s) 575
BlsI GCNGC 4 cut(s) 273, 762, 1357, 1360
Bme1390I CCNGG 1 cut(s) 1310
Bme18I GGWCC 1 cut(s) 1249
BmeRI GACNNNNNGTC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 1249
BmiI GGNNCC 4 cut(s) 882, 1251, 1297, 1367
BmrFI CCNGG 1 cut(s) 1310
BmsI GCATC 3 cut(s) 747, 1037, 1224
BmtI GCTAGC 1 cut(s) 1019
BpiI GAAGAC 3 cut(s) 296, 423, 576
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 2 cut(s) 236, 743
BsaBI GATNNNNATC 1 cut(s) 999
BsaJI CCNNGG 3 cut(s) 504, 575, 1299
BsaXI ACNNNNNCTCC 2 cut(s) 1130, 1160
Bsc4I CCNNNNNNNGG 3 cut(s) 648, 890, 949
Bse1I ACTGG 5 cut(s) 440, 532, 884, 1151, 1369
Bse3DI GCAATG 1 cut(s) 1140
Bse8I GATNNNNATC 1 cut(s) 999
BseBI CCWGG 1 cut(s) 1310
BseDI CCNNGG 3 cut(s) 504, 575, 1299
BseGI GGATG 1 cut(s) 471
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 3 cut(s) 648, 890, 949
BseMI GCAATG 1 cut(s) 1140
BseMII CTCAG 3 cut(s) 878, 1303, 1305
BseNI ACTGG 5 cut(s) 440, 532, 884, 1151, 1369
BseRI GAGGAG 1 cut(s) 363
BseXI GCAGC 3 cut(s) 283, 1367, 1370
BsgI GTGCAG 1 cut(s) 184
Bsh1236I CGCG 1 cut(s) 639
BshFI GGCC 2 cut(s) 364, 928
BshNI GGYRCC 1 cut(s) 1365
BsiHKAI GWGCWC 4 cut(s) 171, 777, 801, 1290
BslFI GGGAC 1 cut(s) 1352
BslI CCNNNNNNNGG 3 cut(s) 648, 890, 949
BsmAI GTCTC 3 cut(s) 333, 683, 867
BsmBI CGTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 1352
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 2 cut(s) 364, 928
Bsp1286I GDGCHC 4 cut(s) 171, 777, 801, 1290
Bsp143I GATC 8 cut(s) 59, 85, 489, 499, 994, 1000, 1295, 1317
BspACI CCGC 4 cut(s) 396, 637, 761, 1076
BspANI GGCC 2 cut(s) 364, 928
BspCNI CTCAG 3 cut(s) 877, 1302, 1306
BspFNI CGCG 1 cut(s) 639
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 4 cut(s) 882, 1251, 1297, 1367
BspOI GCTAGC 1 cut(s) 1019
BspPI GGATC 4 cut(s) 494, 989, 1290, 1303
BspQI GCTCTTC 1 cut(s) 765
BspT107I GGYRCC 1 cut(s) 1365
BspTI CTTAAG 1 cut(s) 764
BsrDI GCAATG 1 cut(s) 1140
BsrI ACTGG 5 cut(s) 440, 532, 884, 1151, 1369
BssECI CCNNGG 3 cut(s) 504, 575, 1299
BssMI GATC 8 cut(s) 59, 85, 489, 499, 994, 1000, 1295, 1317
BssT1I CCWWGG 3 cut(s) 504, 575, 1299
Bst2UI CCWGG 1 cut(s) 1310
Bst4CI ACNGT 5 cut(s) 190, 459, 611, 757, 1058
Bst6I CTCTTC 2 cut(s) 75, 765
BstAFI CTTAAG 1 cut(s) 764
BstC8I GCNNGC 4 cut(s) 831, 1017, 1083, 1205
BstDEI CTNAG 3 cut(s) 864, 1289, 1314
BstENI CCTNNNNNAGG 1 cut(s) 646
BstF5I GGATG 1 cut(s) 471
BstFNI CGCG 1 cut(s) 639
BstH2I RGCGCY 1 cut(s) 1088
BstHHI GCGC 2 cut(s) 641, 1087
BstKTI GATC 8 cut(s) 62, 88, 492, 502, 997, 1003, 1298, 1320
BstMAI GTCTC 3 cut(s) 333, 683, 867
BstMBI GATC 8 cut(s) 59, 85, 489, 499, 994, 1000, 1295, 1317
BstMWI GCNNNNNNNGC 4 cut(s) 271, 1021, 1082, 1310
BstNI CCWGG 1 cut(s) 1310
BstSCI CCNGG 1 cut(s) 1308
BstSFI CTRYAG 1 cut(s) 455
BstUI CGCG 1 cut(s) 639
BstV1I GCAGC 3 cut(s) 283, 1367, 1370
BstV2I GAAGAC 3 cut(s) 296, 423, 576
BstX2I RGATCY 1 cut(s) 1295
BstYI RGATCY 1 cut(s) 1295
BsuRI GGCC 2 cut(s) 364, 928
BtgZI GCGATG 1 cut(s) 535
BtsCI GGATG 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 195, 219, 891
Cac8I GCNNGC 4 cut(s) 831, 1017, 1083, 1205
CaiI CAGNNNCTG 1 cut(s) 311
CciI TCATGA 1 cut(s) 645
CfoI GCGC 2 cut(s) 641, 1087
Cfr13I GGNCC 1 cut(s) 1249
Csp6I GTAC 4 cut(s) 509, 520, 604, 1261
CviAII CATG 5 cut(s) 340, 424, 646, 658, 1168
CviQI GTAC 4 cut(s) 509, 520, 604, 1261
DdeI CTNAG 3 cut(s) 864, 1289, 1314
DpnI GATC 8 cut(s) 61, 87, 491, 501, 996, 1002, 1297, 1319
DpnII GATC 8 cut(s) 59, 85, 489, 499, 994, 1000, 1295, 1317
DraIII CACNNNGTG 1 cut(s) 682
DriI GACNNNNNGTC 1 cut(s) 424
Eam1104I CTCTTC 2 cut(s) 75, 765
Eam1105I GACNNNNNGTC 1 cut(s) 424
EarI CTCTTC 2 cut(s) 75, 765
Ecl136II GAGCTC 1 cut(s) 1288
Eco130I CCWWGG 3 cut(s) 504, 575, 1299
Eco147I AGGCCT 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 1290
Eco32I GATATC 2 cut(s) 345, 1125
Eco47I GGWCC 1 cut(s) 1249
Eco47III AGCGCT 1 cut(s) 1086
Eco53kI GAGCTC 1 cut(s) 1288
EcoICRI GAGCTC 1 cut(s) 1288
EcoNI CCTNNNNNAGG 1 cut(s) 646
EcoRII CCWGG 1 cut(s) 1308
EcoRV GATATC 2 cut(s) 345, 1125
EcoT14I CCWWGG 3 cut(s) 504, 575, 1299
EcoT38I GRGCYC 1 cut(s) 1290
ErhI CCWWGG 3 cut(s) 504, 575, 1299
Esp3I CGTCTC 1 cut(s) 333
FaeI CATG 5 cut(s) 343, 427, 649, 661, 1171
FalI AAGNNNNNCTT 5 cut(s) 36, 165, 197, 770, 802
FaqI GGGAC 1 cut(s) 1352
FatI CATG 5 cut(s) 339, 423, 645, 657, 1167
FauNDI CATATG 1 cut(s) 1394
FbaI TGATCA 2 cut(s) 489, 1000
Fnu4HI GCNGC 4 cut(s) 272, 761, 1356, 1359
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 1290
Fsp4HI GCNGC 4 cut(s) 272, 761, 1356, 1359
FspBI CTAG 4 cut(s) 39, 576, 890, 1016
GlaI GCGC 2 cut(s) 640, 1086
GluI GCNGC 4 cut(s) 272, 761, 1356, 1359
GsuI CTGGAG 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 1088
HaeIII GGCC 2 cut(s) 364, 928
HhaI GCGC 2 cut(s) 641, 1087
Hin1II CATG 5 cut(s) 343, 427, 649, 661, 1171
Hin6I GCGC 2 cut(s) 639, 1085
HinP1I GCGC 2 cut(s) 639, 1085
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 2 cut(s) 92, 624
HphI GGTGA 4 cut(s) 27, 385, 745, 1298
Hpy166II GTNNAC 1 cut(s) 604
Hpy188I TCNGA 4 cut(s) 166, 568, 1038, 1292
Hpy8I GTNNAC 1 cut(s) 604
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 6 cut(s) 226, 387, 652, 871, 940, 1157
HpyCH4III ACNGT 5 cut(s) 190, 459, 611, 757, 1058
HpyCH4IV ACGT 2 cut(s) 583, 1224
HpyCH4V TGCA 6 cut(s) 10, 201, 271, 676, 829, 1276
HpyF10VI GCNNNNNNNGC 4 cut(s) 271, 1021, 1082, 1310
HpyF3I CTNAG 3 cut(s) 864, 1289, 1314
HpySE526I ACGT 2 cut(s) 583, 1224
Hsp92II CATG 5 cut(s) 343, 427, 649, 661, 1171
HspAI GCGC 2 cut(s) 639, 1085
Ksp22I TGATCA 2 cut(s) 489, 1000
Kzo9I GATC 8 cut(s) 59, 85, 489, 499, 994, 1000, 1295, 1317
LguI GCTCTTC 1 cut(s) 765
LmnI GCTCC 4 cut(s) 166, 880, 1078, 1194
Lsp1109I GCAGC 3 cut(s) 283, 1367, 1370
LweI GCATC 3 cut(s) 747, 1037, 1224
MaeI CTAG 4 cut(s) 39, 576, 890, 1016
MaeII ACGT 2 cut(s) 583, 1224
MaeIII GTNAC 4 cut(s) 14, 447, 584, 931
MalI GATC 8 cut(s) 61, 87, 491, 501, 996, 1002, 1297, 1319
MboI GATC 8 cut(s) 59, 85, 489, 499, 994, 1000, 1295, 1317
MfeI CAATTG 2 cut(s) 845, 1211
MflI RGATCY 1 cut(s) 1295
MhlI GDGCHC 4 cut(s) 171, 777, 801, 1290
MluCI AATT 8 cut(s) 177, 301, 845, 897, 937, 1211, 1281, 1333
MmeI TCCRAC 2 cut(s) 1040, 1402
MroXI GAANNNNTTC 2 cut(s) 177, 1146
MseI TTAA 1 cut(s) 765
MslI CAYNNNNRTG 1 cut(s) 338
MspCI CTTAAG 1 cut(s) 764
MspR9I CCNGG 1 cut(s) 1310
MunI CAATTG 2 cut(s) 845, 1211
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 1 cut(s) 1310
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 4 cut(s) 271, 1021, 1082, 1310
NdeI CATATG 1 cut(s) 1394
NdeII GATC 8 cut(s) 59, 85, 489, 499, 994, 1000, 1295, 1317
NheI GCTAGC 1 cut(s) 1015
NlaIII CATG 5 cut(s) 343, 427, 649, 661, 1171
NlaIV GGNNCC 4 cut(s) 882, 1251, 1297, 1367
NmeAIII GCCGAG 1 cut(s) 1342
NmuCI GTSAC 3 cut(s) 447, 584, 931
PagI TCATGA 1 cut(s) 645
PceI AGGCCT 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 765
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 2 cut(s) 177, 1146
PfeI GAWTC 2 cut(s) 92, 624
PkrI GCNGC 4 cut(s) 273, 762, 1357, 1360
Psp124BI GAGCTC 1 cut(s) 1290
Psp6I CCWGG 1 cut(s) 1308
PspGI CCWGG 1 cut(s) 1308
PspN4I GGNNCC 4 cut(s) 882, 1251, 1297, 1367
PspPI GGNCC 1 cut(s) 1249
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 1 cut(s) 1295
RsaI GTAC 4 cut(s) 510, 521, 605, 1262
RsaNI GTAC 4 cut(s) 509, 520, 604, 1261
RseI CAYNNNNRTG 1 cut(s) 338
SacI GAGCTC 1 cut(s) 1290
SapI GCTCTTC 1 cut(s) 765
SaqAI TTAA 1 cut(s) 765
SatI GCNGC 4 cut(s) 272, 761, 1356, 1359
Sau3AI GATC 8 cut(s) 59, 85, 489, 499, 994, 1000, 1295, 1317
Sau96I GGNCC 1 cut(s) 1249
ScrFI CCNGG 1 cut(s) 1310
SduI GDGCHC 4 cut(s) 171, 777, 801, 1290
SfaNI GCATC 3 cut(s) 747, 1037, 1224
SfcI CTRYAG 1 cut(s) 455
SinI GGWCC 1 cut(s) 1249
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 3 cut(s) 251, 722, 764
SmoI CTYRAG 3 cut(s) 251, 722, 764
Sse9I AATT 8 cut(s) 177, 301, 845, 897, 937, 1211, 1281, 1333
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 4 cut(s) 396, 637, 761, 1076
SspI AATATT 1 cut(s) 991
SspMI CTAG 4 cut(s) 39, 576, 890, 1016
SstI GAGCTC 1 cut(s) 1290
StuI AGGCCT 1 cut(s) 364
StyD4I CCNGG 1 cut(s) 1308
StyI CCWWGG 3 cut(s) 504, 575, 1299
TaaI ACNGT 5 cut(s) 190, 459, 611, 757, 1058
TaiI ACGT 2 cut(s) 586, 1227
TaqI TCGA 4 cut(s) 47, 984, 1239, 1265
TasI AATT 8 cut(s) 177, 301, 845, 897, 937, 1211, 1281, 1333
TatI WGTACW 2 cut(s) 519, 1260
TauI GCSGC 1 cut(s) 763
TfiI GAWTC 2 cut(s) 92, 624
Tru1I TTAA 1 cut(s) 765
Tru9I TTAA 1 cut(s) 765
TscAI CASTG 3 cut(s) 195, 226, 891
TseFI GTSAC 3 cut(s) 447, 584, 931
TseI GCWGC 3 cut(s) 271, 1355, 1358
Tsp45I GTSAC 3 cut(s) 447, 584, 931
TspDTI ATGAA 6 cut(s) 123, 138, 429, 634, 674, 1381
TspRI CASTG 3 cut(s) 195, 226, 891
Vha464I CTTAAG 1 cut(s) 764
VpaK11BI GGWCC 1 cut(s) 1249
XagI CCTNNNNNAGG 1 cut(s) 646
XapI RAATTY 2 cut(s) 177, 301
XmaJI CCTAGG 1 cut(s) 575
XmnI GAANNNNTTC 2 cut(s) 177, 1146
XspI CTAG 4 cut(s) 39, 576, 890, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.