pycom10g10970

Serine threonine protein phosphatase 2A 57 kDa regulatory subunit B' beta isoform-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015544
Physical Location & Seq
Reverse (-)
14514532 .. 14516757
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g10970.1

Sequence Viewer

Length: 1434 bp
ATGGGAGTTCAAAACGACGCTCCAAGAGTGTCACCAAGGGCTTCGCCAAGGGCTTCCCCAAGAGGAAAATCCGCAACCCTTCAACACCTATTTGATCTCGATGCCAAAAGCACTTCTGACTGCGGAAAAAAGATTGCCAATTCCAATAGTACTATTGGCAGTCAATCTTCTTCCAGTGTGGAAAATGAAGAAATCCTATCAGCAATCTCCGTCTGCCATTTTATCTTCACCTTCACTGATCCAAGCGAATGCCCAACGCAACAAGAACTCAAAGGGCAAAAGCTTATGCAGCTCCTCTCAATTGTCAAAACCTCGAAAAAGCCTCTGCATGATCATGTCATGTCGTCTCTCATTTCCATGATATCGTTAAACCTTTTCCGGCCTCTCCCTCCTCCTTCCAATCCCACAATCGCTACGGACTTTCCCGATGATGAGGACCCCATATCCTCCTTTTCCCCTGTGTGGTCGCACCTGCAGTTAGTCTACGACATCCTCATCCGCATCGTGATCAACAACGATCCTAAGTTACTCAGAGGATACATTGACCACCAGTTTGTTGTCAACCTCCTTGTCCTCTTCCAATCAGAAGACCCGAGGGAGCGTGACAGCTTGAAGAATGTGTACCATAAAATATATTCTCGATTCACATTCTACCGTTCCTTCATGAGGAAATCTATGAACGATGTGTTCTTGCACTATGTGTTTGAGGCAGAGCGGCATTGTGGGATCAGAGAGTTGCTTGAGATATGGGGAAGCATAATCAATGGCTTCACTGTGCCGCTGAAGGAAGAGCACAAGCTGTTTTTCATGAGAGTGCTTATTCCTTTCCACAAGGCTAAAGGGATGCAGAGCTACCACAGGCAGCTGGCTTATTGCGTGTCGCAGTTTGTGCAGAAGGAGCCCGTGCTTGGCGGGATAGTGGTGAGAGGGATTTTGAGGTATTGGCCCGTGACGAATTGCCAGAAGGAGGTTTTGCTGGTTGGGGAGCTGGAGGAGCTGGTGGAGAATATTGACCCTGATCAGTATAGGAAGTTGGCTCTGCCTTTATGCCTACGGATAACAAGATGCTTGAACAGTTGGAACTCACAGGTAGCAGAGAGAGCCCTCTATGTTTGGAACAATGAGCACTTTGTGAAGATGATATCTGTGGCAATGGAGGAAGTGTTTCCGGTCGTGGTTGAAGGCATGGAGAAGAACTTGAAGTGGCATTGGAGCAAAAGCGTCCTGCAACTGACCGAAAACGTGAAGGTGATGCTTGAAGAAATGGATCCAGTTTTGTACTCCAAATGCCTTGAAGAAATTGAGCTAAGAGAATCCAAGGCCAACCAAGCAGAAATGAAGAGGAAGGAGAAATGGGACAGAATAGAAATGGCAGCCGCAGCCAAGCCTCAGTTCCTCCAACCACAACATTTCTTGTGTGTATACAACAAATAG

Protein Analysis

478

Amino Acids

55.09

Weight (kDa)

7.61

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B56 PF01603 56 - 459 3e-144 Protein phosphatase 2A regulatory B subunit (B56 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015544)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g15670
malus_domestica MD05G1125100.v1.1
prunus_persica Prupe.8G169200_v2.0.a1
pyrus_communis pycom05g11940 pycom10g10970
rosa_chinensis RchiOBHm_Chr6g0278971
rosa_laevigata RLG00000013179
rosa_multiflora Rmu_sc0002080.1_g000042 Rmu_sc0009618.1_g000015
rosa_roxburghii Rroxscaffold_7G00189300
rosa_rugosa Rorug06G0120500
rosa_samantha Rh6AG229200 Rh6BG234400 Rh6CG236600 Rh6DG228200
rosa_wichuraiana Rw6G020070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 480
Acc36I ACCTGC 1 cut(s) 480
AccBSI CCGCTC 1 cut(s) 715
AccI GTMKAC 2 cut(s) 483, 1422
AciI CCGC 7 cut(s) 72, 123, 499, 715, 779, 912, 1377
AclWI GGATC 5 cut(s) 233, 512, 734, 1262, 1275
AcuI CTGAAG 1 cut(s) 803
AdeI CACNNNGTG 2 cut(s) 700, 1132
AfaI GTAC 3 cut(s) 151, 623, 1280
AfiI CCNNNNNNNGG 4 cut(s) 462, 666, 908, 967
AgsI TTSAA 8 cut(s) 11, 83, 613, 1072, 1181, 1201, 1259, 1295
AjuI GAANNNNNNNTTGG 2 cut(s) 1376, 1408
AluBI AGCT 9 cut(s) 283, 292, 609, 799, 852, 865, 988, 997, 1306
AluI AGCT 9 cut(s) 283, 292, 609, 799, 852, 865, 988, 997, 1306
Alw21I GWGCWC 2 cut(s) 795, 1128
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 5 cut(s) 233, 512, 734, 1262, 1275
Ama87I CYCGRG 1 cut(s) 592
AoxI GGCC 3 cut(s) 380, 944, 1320
ApeKI GCWGC 4 cut(s) 289, 862, 1373, 1379
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
Asp700I GAANNNNTTC 1 cut(s) 1164
AspS9I GGNCC 2 cut(s) 436, 945
AsuHPI GGTGA 4 cut(s) 24, 220, 934, 1261
AvaI CYCGRG 1 cut(s) 592
AvaII GGWCC 1 cut(s) 436
BamHI GGATCC 1 cut(s) 1267
BanII GRGCYC 2 cut(s) 903, 1105
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BbsI GAAGAC 1 cut(s) 594
Bbv12I GWGCWC 2 cut(s) 795, 1128
BbvI GCAGC 4 cut(s) 301, 874, 1385, 1391
BciVI GTATCC 1 cut(s) 530
BclI TGATCA 3 cut(s) 331, 507, 1018
BcoDI GTCTC 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 480
BfuI GTATCC 1 cut(s) 530
BisI GCNGC 7 cut(s) 290, 716, 779, 863, 1374, 1377, 1380
BlsI GCNGC 7 cut(s) 291, 717, 780, 864, 1375, 1378, 1381
BmcAI AGTACT 1 cut(s) 151
Bme18I GGWCC 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 592
BmgT120I GGNCC 2 cut(s) 436, 945
BmiI GGNNCC 3 cut(s) 438, 900, 1269
BmsI GCATC 5 cut(s) 91, 510, 834, 1055, 1242
BpiI GAAGAC 1 cut(s) 594
BpmI CTGGAG 1 cut(s) 1010
BpuEI CTTGAG 1 cut(s) 761
BsaJI CCNNGG 4 cut(s) 35, 47, 593, 1317
BsaWI WCCGGW 1 cut(s) 1168
BsaXI ACNNNNNCTCC 2 cut(s) 1148, 1178
Bsc4I CCNNNNNNNGG 4 cut(s) 462, 666, 908, 967
Bse1I ACTGG 3 cut(s) 174, 550, 1271
Bse3DI GCAATG 1 cut(s) 1158
BseDI CCNNGG 4 cut(s) 35, 47, 593, 1317
BseGI GGATG 3 cut(s) 489, 495, 849
BseLI CCNNNNNNNGG 4 cut(s) 462, 666, 908, 967
BseMI GCAATG 1 cut(s) 1158
BseMII CTCAG 2 cut(s) 544, 1403
BseNI ACTGG 3 cut(s) 174, 550, 1271
BseRI GAGGAG 3 cut(s) 284, 381, 1007
BseXI GCAGC 4 cut(s) 301, 874, 1385, 1391
BsgI GTGCAG 1 cut(s) 911
Bsh1285I CGRYCG 1 cut(s) 1173
BshFI GGCC 3 cut(s) 382, 946, 1322
BsiEI CGRYCG 1 cut(s) 1173
BsiHKAI GWGCWC 2 cut(s) 795, 1128
BsiHKCI CYCGRG 1 cut(s) 592
BsiSI CCGG 2 cut(s) 379, 1169
BslFI GGGAC 1 cut(s) 1370
BslI CCNNNNNNNGG 4 cut(s) 462, 666, 908, 967
BsmAI GTCTC 1 cut(s) 351
BsmBI CGTCTC 1 cut(s) 351
BsmFI GGGAC 1 cut(s) 1370
BsmI GAATGC 1 cut(s) 254
BsnI GGCC 3 cut(s) 382, 946, 1322
BsoBI CYCGRG 1 cut(s) 592
Bsp1286I GDGCHC 4 cut(s) 795, 903, 1105, 1128
Bsp143I GATC 8 cut(s) 94, 238, 331, 507, 517, 726, 1018, 1267
BspACI CCGC 7 cut(s) 72, 123, 499, 715, 779, 912, 1377
BspANI GGCC 3 cut(s) 382, 946, 1322
BspCNI CTCAG 2 cut(s) 543, 1402
BspHI TCATGA 2 cut(s) 663, 807
BspLI GGNNCC 3 cut(s) 438, 900, 1269
BspMAI CTGCAG 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 480
BspPI GGATC 5 cut(s) 233, 512, 734, 1262, 1275
BspQI GCTCTTC 1 cut(s) 783
BsrBI CCGCTC 1 cut(s) 715
BsrDI GCAATG 1 cut(s) 1158
BsrI ACTGG 3 cut(s) 174, 550, 1271
BssECI CCNNGG 4 cut(s) 35, 47, 593, 1317
BssMI GATC 8 cut(s) 94, 238, 331, 507, 517, 726, 1018, 1267
BssNAI GTATAC 1 cut(s) 1423
BssT1I CCWWGG 3 cut(s) 35, 47, 1317
Bst1107I GTATAC 1 cut(s) 1423
Bst4CI ACNGT 3 cut(s) 656, 775, 1076
Bst6I CTCTTC 3 cut(s) 581, 783, 1334
BstAPI GCANNNNNTGC 1 cut(s) 889
BstC8I GCNNGC 1 cut(s) 867
BstDEI CTNAG 4 cut(s) 522, 530, 1307, 1389
BstENI CCTNNNNNAGG 1 cut(s) 664
BstF5I GGATG 3 cut(s) 489, 495, 849
BstKTI GATC 8 cut(s) 97, 241, 334, 510, 520, 729, 1021, 1270
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 8 cut(s) 94, 238, 331, 507, 517, 726, 1018, 1267
BstMCI CGRYCG 1 cut(s) 1173
BstMWI GCNNNNNNNGC 7 cut(s) 289, 889, 898, 994, 1100, 1328, 1379
BstSFI CTRYAG 1 cut(s) 473
BstV1I GCAGC 4 cut(s) 301, 874, 1385, 1391
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 1 cut(s) 1267
BstYI RGATCY 1 cut(s) 1267
BstZ17I GTATAC 1 cut(s) 1423
BsuI GTATCC 1 cut(s) 530
BsuRI GGCC 3 cut(s) 382, 946, 1322
BtsCI GGATG 3 cut(s) 489, 495, 849
BtsIMutI CAGTG 3 cut(s) 181, 234, 771
BveI ACCTGC 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 867
CciI TCATGA 2 cut(s) 663, 807
Cfr13I GGNCC 2 cut(s) 436, 945
CseI GACGC 2 cut(s) 26, 1210
Csp6I GTAC 3 cut(s) 150, 622, 1279
CviAII CATG 7 cut(s) 329, 335, 340, 358, 664, 808, 1186
CviQI GTAC 3 cut(s) 150, 622, 1279
DdeI CTNAG 4 cut(s) 522, 530, 1307, 1389
DpnI GATC 8 cut(s) 96, 240, 333, 509, 519, 728, 1020, 1269
DpnII GATC 8 cut(s) 94, 238, 331, 507, 517, 726, 1018, 1267
DraIII CACNNNGTG 2 cut(s) 700, 1132
Eam1104I CTCTTC 3 cut(s) 581, 783, 1334
EarI CTCTTC 3 cut(s) 581, 783, 1334
Eco130I CCWWGG 3 cut(s) 35, 47, 1317
Eco24I GRGCYC 2 cut(s) 903, 1105
Eco32I GATATC 2 cut(s) 363, 1143
Eco47I GGWCC 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 803
Eco88I CYCGRG 1 cut(s) 592
EcoNI CCTNNNNNAGG 1 cut(s) 664
EcoO109I RGGNCCY 1 cut(s) 436
EcoRV GATATC 2 cut(s) 363, 1143
EcoT14I CCWWGG 3 cut(s) 35, 47, 1317
EcoT38I GRGCYC 2 cut(s) 903, 1105
ErhI CCWWGG 3 cut(s) 35, 47, 1317
Esp3I CGTCTC 1 cut(s) 351
FaeI CATG 7 cut(s) 332, 338, 343, 361, 667, 811, 1189
FaqI GGGAC 1 cut(s) 1370
FatI CATG 7 cut(s) 328, 334, 339, 357, 663, 807, 1185
FauI CCCGC 1 cut(s) 905
FbaI TGATCA 3 cut(s) 331, 507, 1018
FblI GTMKAC 2 cut(s) 483, 1422
Fnu4HI GCNGC 7 cut(s) 290, 716, 779, 863, 1374, 1377, 1380
FokI GGATG 3 cut(s) 476, 482, 856
FriOI GRGCYC 2 cut(s) 903, 1105
Fsp4HI GCNGC 7 cut(s) 290, 716, 779, 863, 1374, 1377, 1380
GluI GCNGC 7 cut(s) 290, 716, 779, 863, 1374, 1377, 1380
GsuI CTGGAG 1 cut(s) 1010
HaeIII GGCC 3 cut(s) 382, 946, 1322
HapII CCGG 2 cut(s) 379, 1169
HgaI GACGC 2 cut(s) 26, 1210
Hin1II CATG 7 cut(s) 332, 338, 343, 361, 667, 811, 1189
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 2 cut(s) 642, 1313
HpaII CCGG 2 cut(s) 379, 1169
HphI GGTGA 4 cut(s) 24, 220, 934, 1261
Hpy166II GTNNAC 4 cut(s) 484, 562, 622, 1423
Hpy188I TCNGA 4 cut(s) 118, 533, 586, 731
Hpy188III TCNNGA 6 cut(s) 98, 425, 505, 639, 664, 808
Hpy8I GTNNAC 4 cut(s) 484, 562, 622, 1423
Hpy99I CGWCG 1 cut(s) 20
HpyCH4III ACNGT 3 cut(s) 656, 775, 1076
HpyCH4IV ACGT 1 cut(s) 1242
HpyCH4V TGCA 7 cut(s) 289, 328, 475, 694, 847, 892, 1228
HpyF10VI GCNNNNNNNGC 7 cut(s) 289, 889, 898, 994, 1100, 1328, 1379
HpyF3I CTNAG 4 cut(s) 522, 530, 1307, 1389
HpySE526I ACGT 1 cut(s) 1242
Hsp92II CATG 7 cut(s) 332, 338, 343, 361, 667, 811, 1189
Ksp22I TGATCA 3 cut(s) 331, 507, 1018
Kzo9I GATC 8 cut(s) 94, 238, 331, 507, 517, 726, 1018, 1267
LguI GCTCTTC 1 cut(s) 783
LmnI GCTCC 7 cut(s) 25, 297, 598, 898, 985, 994, 1212
Lsp1109I GCAGC 4 cut(s) 301, 874, 1385, 1391
LweI GCATC 5 cut(s) 91, 510, 834, 1055, 1242
MaeII ACGT 1 cut(s) 1242
MaeIII GTNAC 4 cut(s) 30, 525, 602, 949
MalI GATC 8 cut(s) 96, 240, 333, 509, 519, 728, 1020, 1269
MbiI CCGCTC 1 cut(s) 715
MboI GATC 8 cut(s) 94, 238, 331, 507, 517, 726, 1018, 1267
MfeI CAATTG 1 cut(s) 300
MflI RGATCY 1 cut(s) 1267
MhlI GDGCHC 4 cut(s) 795, 903, 1105, 1128
MluCI AATT 4 cut(s) 139, 300, 955, 1299
MmeI TCCRAC 2 cut(s) 1058, 1423
MroXI GAANNNNTTC 1 cut(s) 1164
MseI TTAA 1 cut(s) 368
MslI CAYNNNNRTG 3 cut(s) 333, 356, 812
MspA1I CMGCKG 2 cut(s) 781, 865
MspI CCGG 2 cut(s) 379, 1169
MunI CAATTG 1 cut(s) 300
Mva1269I GAATGC 1 cut(s) 254
MwoI GCNNNNNNNGC 7 cut(s) 289, 889, 898, 994, 1100, 1328, 1379
NdeII GATC 8 cut(s) 94, 238, 331, 507, 517, 726, 1018, 1267
NlaIII CATG 7 cut(s) 332, 338, 343, 361, 667, 811, 1189
NlaIV GGNNCC 3 cut(s) 438, 900, 1269
NmuCI GTSAC 3 cut(s) 30, 602, 949
PagI TCATGA 2 cut(s) 663, 807
PaqCI CACCTGC 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 783
PctI GAATGC 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 1164
PfeI GAWTC 2 cut(s) 642, 1313
PkrI GCNGC 7 cut(s) 291, 717, 780, 864, 1375, 1378, 1381
PpuMI RGGWCCY 1 cut(s) 436
Psp5II RGGWCCY 1 cut(s) 436
PspN4I GGNNCC 3 cut(s) 438, 900, 1269
PspPI GGNCC 2 cut(s) 436, 945
PspPPI RGGWCCY 1 cut(s) 436
PstI CTGCAG 1 cut(s) 477
PsuI RGATCY 1 cut(s) 1267
PvuII CAGCTG 1 cut(s) 865
RsaI GTAC 3 cut(s) 151, 623, 1280
RsaNI GTAC 3 cut(s) 150, 622, 1279
RseI CAYNNNNRTG 3 cut(s) 333, 356, 812
SapI GCTCTTC 1 cut(s) 783
SaqAI TTAA 1 cut(s) 368
SatI GCNGC 7 cut(s) 290, 716, 779, 863, 1374, 1377, 1380
Sau3AI GATC 8 cut(s) 94, 238, 331, 507, 517, 726, 1018, 1267
Sau96I GGNCC 2 cut(s) 436, 945
ScaI AGTACT 1 cut(s) 151
SduI GDGCHC 4 cut(s) 795, 903, 1105, 1128
SfaNI GCATC 5 cut(s) 91, 510, 834, 1055, 1242
SfcI CTRYAG 1 cut(s) 473
SinI GGWCC 1 cut(s) 436
SmiMI CAYNNNNRTG 3 cut(s) 333, 356, 812
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 4 cut(s) 139, 300, 955, 1299
SsiI CCGC 7 cut(s) 72, 123, 499, 715, 779, 912, 1377
SspI AATATT 1 cut(s) 1009
StyI CCWWGG 3 cut(s) 35, 47, 1317
TaaI ACNGT 3 cut(s) 656, 775, 1076
TaiI ACGT 1 cut(s) 1245
TaqI TCGA 3 cut(s) 99, 314, 640
TaqII GACCGA 1 cut(s) 1250
TasI AATT 4 cut(s) 139, 300, 955, 1299
TatI WGTACW 2 cut(s) 149, 1278
TauI GCSGC 3 cut(s) 718, 781, 1379
TfiI GAWTC 2 cut(s) 642, 1313
Tru1I TTAA 1 cut(s) 368
Tru9I TTAA 1 cut(s) 368
TscAI CASTG 3 cut(s) 181, 241, 778
TseFI GTSAC 3 cut(s) 30, 602, 949
TseI GCWGC 4 cut(s) 289, 862, 1373, 1379
Tsp45I GTSAC 3 cut(s) 30, 602, 949
TspDTI ATGAA 5 cut(s) 201, 652, 692, 796, 1352
TspGWI ACGGA 3 cut(s) 199, 431, 1069
TspRI CASTG 3 cut(s) 181, 241, 778
VpaK11BI GGWCC 1 cut(s) 436
XagI CCTNNNNNAGG 1 cut(s) 664
XmiI GTMKAC 2 cut(s) 483, 1422
XmnI GAANNNNTTC 1 cut(s) 1164
ZrmI AGTACT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.