FvH4_2g16360

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015401
Physical Location & Seq
Reverse (-)
14285977 .. 14289014
3038 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g16360.t1

Sequence Viewer

Length: 1182 bp
ATGGCCATGTCTTGGAGGCTTCCAACTCAGCTGGCAACCAAACCGCCCCATCACCACGACCCCAAACTCTCATGGTGCCGCACTTTAGCACCAGACCAACTCCATCTCACTTCTTCCGCTTCTTCACTCTCCACCACCACTGGGAGTTGTACTAGTCGAGGTTTGGTGAGATGTACGGCCAGGACAGGAGTCGGGGCTGTGAATTTGGCTCCGGGGACACCTGTCAGGCCCACCAGTATACTAGTGGTTGGCGCCACCGGAACGCTGGGGAGGCAGATTGTGAGGCGAGCGTTGGATGAAGGGTATGACGTTAGGTGCTTGGTTAGGCCTAGACCCGCTCCGGCTGACTTTCTCAGAGATTGGGGCGCTACTGTTGTCAATGCAGACCTTAGCAAACCGGAGACTATACCTGCAACATTGGTTGGTGTTCATACAGTTATTGATTGTGCCACTGGCCGTCCAGAGGAGCCCATCAAAAAGGTAGATTGGGAAGGAAAAGTTGCTCTTATACAATGTGCAAAGGCAATGGGGATCCAGAAATTTGTGTTCTTTTCTATCCACAACTGTGACAAGCATCCTGAAGTTCCTCTTATGGAGATCAAGTACTGCACTGAAAAGTTTCTCAAGGATTCAGGCATAAACCATGTTATAATTCGTGCATGTGGATTCATGCAAGGGATTATTGGGCAGTATGCAGTACCTATATTGGAAGAGAAATCAGTTTGGGGAACAGATGCCCCTACAAGAATTGCATACATGGACACCCAGGATATAGCTCGGTTGACATTTATAGCTTTACGAAATGAGAATGTTAATGGGAAGCTTCTCACATTTGCTGGCCCTCGTGCATGGACAACCCAAGAGGTAATAACATTGTGTGAAAGGTTTGCGGGGCAAGAAGCAAACATTACCACAGTTCCTGTCTCTGTCTTAAGAGTAACCCGTCAGCTGACTAGGCTTTTTGAGTGGACAAATGATGTTGCCGATAGATTGGCATTTTCAGAGGTCCTTTCAAGCGATACTGTTTTCTCTGCTCCAATGAACGAGACATATAGTCTTCTTGGTGTGGACGCAAAAGATGTAGTTACTCTTGAGAAATATTTGCAAGACTACTTCACCAACATATTGAAGAAGTTGAAGGATCTGAAAGCACAATCTAAGCAATCTGACATTTACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

394

Amino Acids

43.48

Weight (kDa)

8.61

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 79 - 328 2e-28 NmrA-like family
Epimerase PF01370 80 - 185 5.6e-10 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 84 - 233 1.6e-17 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015401)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35250
fragaria_vesca FvH4_2g16360 FvH4_2g16360 FvH4_2g16360
malus_domestica MD05G1131800.v1.1
prunus_persica Prupe.8G176300_v2.0.a1
pyrus_communis pycom05g12860
rosa_chinensis RchiOBHm_Chr6g0280111
rosa_laevigata RLG00000013088
rosa_multiflora Rmu_sc0007137.1_g000006
rosa_roxburghii Rroxscaffold_7G00188370 Rroxscaffold_7G00189360
rosa_rugosa Rorug06G0130000
rosa_samantha Rh6AG240300 Rh6BG244100
rosa_wichuraiana Rw6G020940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 650
Acc36I ACCTGC 1 cut(s) 418
AccB1I GGYRCC 2 cut(s) 75, 251
AccB7I CCANNNNNTGG 1 cut(s) 12
AccBSI CCGCTC 1 cut(s) 338
AccI GTMKAC 1 cut(s) 238
AciI CCGC 5 cut(s) 44, 79, 117, 336, 890
AclWI GGATC 3 cut(s) 526, 539, 1149
AcoI YGGCCR 3 cut(s) 3, 177, 454
AcsI RAATTY 2 cut(s) 202, 539
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 4 cut(s) 151, 175, 605, 699
AfiI CCNNNNNNNGG 3 cut(s) 12, 141, 463
AflII CTTAAG 1 cut(s) 931
AgsI TTSAA 3 cut(s) 1014, 1129, 1138
AhdI GACNNNNNGTC 1 cut(s) 1053
AhlI ACTAGT 2 cut(s) 152, 241
AjnI CCWGG 2 cut(s) 179, 765
AleI CACNNNNGTG 1 cut(s) 564
AluBI AGCT 5 cut(s) 31, 776, 794, 823, 949
AluI AGCT 5 cut(s) 31, 776, 794, 823, 949
Alw26I GTCTC 3 cut(s) 395, 928, 1040
AlwI GGATC 3 cut(s) 526, 539, 1149
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 6 cut(s) 3, 177, 227, 326, 454, 838
ApoI RAATTY 2 cut(s) 202, 539
Asp700I GAANNNNTTC 1 cut(s) 618
AspLEI GCGC 2 cut(s) 254, 368
AspS9I GGNCC 3 cut(s) 228, 839, 1006
AsuC2I CCSGG 1 cut(s) 213
AsuHPI GGTGA 3 cut(s) 44, 178, 1108
AvaII GGWCC 1 cut(s) 1006
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 531
BanI GGYRCC 2 cut(s) 75, 251
BanII GRGCYC 1 cut(s) 471
BauI CACGAG 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 1049
BccI CCATC 3 cut(s) 57, 111, 479
BceAI ACGGC 2 cut(s) 192, 441
BciT130I CCWGG 2 cut(s) 181, 767
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 3 cut(s) 395, 928, 1040
BcuI ACTAGT 2 cut(s) 152, 241
BfaI CTAG 4 cut(s) 153, 242, 330, 954
BfoI RGCGCY 2 cut(s) 255, 369
BfrI CTTAAG 1 cut(s) 931
BfuAI ACCTGC 1 cut(s) 418
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
BmcAI AGTACT 1 cut(s) 605
Bme1390I CCNGG 3 cut(s) 181, 213, 767
Bme18I GGWCC 1 cut(s) 1006
BmeRI GACNNNNNGTC 1 cut(s) 1053
BmgT120I GGNCC 3 cut(s) 228, 839, 1006
BmiI GGNNCC 5 cut(s) 77, 210, 253, 468, 533
BmrFI CCNGG 3 cut(s) 181, 213, 767
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 2 cut(s) 583, 724
BmuI ACTGGG 1 cut(s) 150
BoxI GACNNNNGTC 2 cut(s) 188, 221
BpiI GAAGAC 1 cut(s) 1049
Bpu10I CCTNAGC 1 cut(s) 389
BpuEI CTTGAG 2 cut(s) 608, 1112
BpuMI CCSGG 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 2 cut(s) 212, 765
BsaWI WCCGGW 2 cut(s) 257, 397
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 141, 463
Bse1I ACTGG 3 cut(s) 145, 234, 457
Bse3DI GCAATG 1 cut(s) 531
BseBI CCWGG 2 cut(s) 181, 767
BseDI CCNNGG 2 cut(s) 212, 765
BseGI GGATG 2 cut(s) 301, 574
BseLI CCNNNNNNNGG 3 cut(s) 12, 141, 463
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 2 cut(s) 41, 367
BseNI ACTGG 3 cut(s) 145, 234, 457
BseRI GAGGAG 1 cut(s) 479
BseYI CCCAGC 1 cut(s) 265
BsgI GTGCAG 1 cut(s) 592
BshFI GGCC 6 cut(s) 5, 179, 229, 328, 456, 840
BshNI GGYRCC 2 cut(s) 75, 251
BsiSI CCGG 4 cut(s) 212, 258, 341, 398
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 3 cut(s) 12, 141, 463
BsmAI GTCTC 3 cut(s) 395, 928, 1040
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 6 cut(s) 5, 179, 229, 328, 456, 840
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 3 cut(s) 531, 597, 1141
BspACI CCGC 5 cut(s) 44, 79, 117, 336, 890
BspANI GGCC 6 cut(s) 5, 179, 229, 328, 456, 840
BspCNI CTCAG 2 cut(s) 40, 366
BspLI GGNNCC 5 cut(s) 77, 210, 253, 468, 533
BspMI ACCTGC 1 cut(s) 418
BspPI GGATC 3 cut(s) 526, 539, 1149
BspT107I GGYRCC 2 cut(s) 75, 251
BspTI CTTAAG 1 cut(s) 931
BsrBI CCGCTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 531
BsrI ACTGG 3 cut(s) 145, 234, 457
BssECI CCNNGG 2 cut(s) 212, 765
BssMI GATC 3 cut(s) 531, 597, 1141
BssNAI GTATAC 1 cut(s) 239
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 843
Bst1107I GTATAC 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 843
Bst2UI CCWGG 2 cut(s) 181, 767
Bst4CI ACNGT 5 cut(s) 373, 436, 566, 916, 1024
Bst6I CTCTTC 1 cut(s) 705
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 931
BstC8I GCNNGC 3 cut(s) 33, 288, 838
BstDEI CTNAG 4 cut(s) 27, 353, 389, 1158
BstF5I GGATG 2 cut(s) 301, 574
BstH2I RGCGCY 2 cut(s) 255, 369
BstHHI GCGC 2 cut(s) 254, 368
BstKTI GATC 3 cut(s) 534, 600, 1144
BstMAI GTCTC 3 cut(s) 395, 928, 1040
BstMBI GATC 3 cut(s) 531, 597, 1141
BstMWI GCNNNNNNNGC 2 cut(s) 271, 955
BstNI CCWGG 2 cut(s) 181, 767
BstNSI RCATGY 1 cut(s) 663
BstPAI GACNNNNGTC 2 cut(s) 188, 221
BstSCI CCNGG 3 cut(s) 179, 211, 765
BstV2I GAAGAC 1 cut(s) 1049
BstX2I RGATCY 2 cut(s) 531, 1141
BstYI RGATCY 2 cut(s) 531, 1141
BstZ17I GTATAC 1 cut(s) 239
BsuRI GGCC 6 cut(s) 5, 179, 229, 328, 456, 840
BtsCI GGATG 2 cut(s) 301, 574
BtsIMutI CAGTG 3 cut(s) 138, 450, 609
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 3 cut(s) 33, 288, 838
CaiI CAGNNNCTG 1 cut(s) 920
CfoI GCGC 2 cut(s) 254, 368
Cfr13I GGNCC 3 cut(s) 228, 839, 1006
CseI GACGC 1 cut(s) 1079
Csp6I GTAC 4 cut(s) 150, 174, 604, 698
CviAII CATG 7 cut(s) 7, 72, 644, 660, 670, 757, 849
CviQI GTAC 4 cut(s) 150, 174, 604, 698
DdeI CTNAG 4 cut(s) 27, 353, 389, 1158
DinI GGCGCC 1 cut(s) 253
DpnI GATC 3 cut(s) 533, 599, 1143
DpnII GATC 3 cut(s) 531, 597, 1141
DriI GACNNNNNGTC 1 cut(s) 1053
EaeI YGGCCR 3 cut(s) 3, 177, 454
Eam1104I CTCTTC 1 cut(s) 705
Eam1105I GACNNNNNGTC 1 cut(s) 1053
EarI CTCTTC 1 cut(s) 705
Eco147I AGGCCT 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 1 cut(s) 1006
Eco57I CTGAAG 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 1006
EcoRII CCWGG 2 cut(s) 179, 765
EcoT38I GRGCYC 1 cut(s) 471
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
FaeI CATG 7 cut(s) 10, 75, 647, 663, 673, 760, 852
FalI AAGNNNNNCTT 4 cut(s) 489, 521, 573, 605
FaqI GGGAC 1 cut(s) 229
FatI CATG 7 cut(s) 6, 71, 643, 659, 669, 756, 848
FauI CCCGC 2 cut(s) 343, 883
FblI GTMKAC 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 2 cut(s) 308, 561
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 4 cut(s) 153, 242, 330, 954
GlaI GCGC 2 cut(s) 253, 367
GluI GCNGC 1 cut(s) 79
GsaI CCCAGC 1 cut(s) 269
HaeII RGCGCY 2 cut(s) 255, 369
HaeIII GGCC 6 cut(s) 5, 179, 229, 328, 456, 840
HapII CCGG 4 cut(s) 212, 258, 341, 398
HgaI GACGC 1 cut(s) 1079
HhaI GCGC 2 cut(s) 254, 368
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 7 cut(s) 10, 75, 647, 663, 673, 760, 852
Hin6I GCGC 2 cut(s) 252, 366
HinP1I GCGC 2 cut(s) 252, 366
HincII GTYRAC 1 cut(s) 783
HindII GTYRAC 1 cut(s) 783
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 3 cut(s) 189, 629, 666
HpaII CCGG 4 cut(s) 212, 258, 341, 398
HphI GGTGA 3 cut(s) 44, 178, 1108
Hpy166II GTNNAC 4 cut(s) 239, 783, 969, 1069
Hpy188I TCNGA 4 cut(s) 356, 1003, 1146, 1168
Hpy188III TCNNGA 4 cut(s) 461, 535, 578, 1091
Hpy8I GTNNAC 4 cut(s) 239, 783, 969, 1069
HpyAV CCTTC 3 cut(s) 293, 485, 1132
HpyCH4III ACNGT 5 cut(s) 373, 436, 566, 916, 1024
HpyCH4IV ACGT 1 cut(s) 309
HpyF10VI GCNNNNNNNGC 2 cut(s) 271, 955
HpyF3I CTNAG 4 cut(s) 27, 353, 389, 1158
HpySE526I ACGT 1 cut(s) 309
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 7 cut(s) 10, 75, 647, 663, 673, 760, 852
HspAI GCGC 2 cut(s) 252, 366
KasI GGCGCC 1 cut(s) 251
Kzo9I GATC 3 cut(s) 531, 597, 1141
LmnI GCTCC 4 cut(s) 214, 343, 466, 1039
LweI GCATC 2 cut(s) 583, 724
MaeI CTAG 4 cut(s) 153, 242, 330, 954
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 3 cut(s) 566, 937, 1084
MalI GATC 3 cut(s) 533, 599, 1143
MbiI CCGCTC 1 cut(s) 338
MboI GATC 3 cut(s) 531, 597, 1141
MboII GAAGA 5 cut(s) 105, 114, 722, 1049, 1141
MflI RGATCY 2 cut(s) 531, 1141
MhlI GDGCHC 1 cut(s) 471
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 202, 539, 651, 747
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 2 cut(s) 47, 273
MnlI CCTC 9 cut(s) 9, 152, 264, 276, 457, 597, 852, 856, 997
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 618
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 813, 932
MslI CAYNNNNRTG 1 cut(s) 564
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 31, 949
MspCI CTTAAG 1 cut(s) 931
MspI CCGG 4 cut(s) 212, 258, 341, 398
MspR9I CCNGG 3 cut(s) 181, 213, 767
MvaI CCWGG 2 cut(s) 181, 767
MwoI GCNNNNNNNGC 2 cut(s) 271, 955
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 1 cut(s) 213
NdeII GATC 3 cut(s) 531, 597, 1141
NlaIII CATG 7 cut(s) 10, 75, 647, 663, 673, 760, 852
NlaIV GGNNCC 5 cut(s) 77, 210, 253, 468, 533
NmuCI GTSAC 1 cut(s) 566
NspI RCATGY 1 cut(s) 663
OliI CACNNNNGTG 1 cut(s) 564
PceI AGGCCT 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 618
PfeI GAWTC 2 cut(s) 629, 666
PflMI CCANNNNNTGG 1 cut(s) 12
PkrI GCNGC 1 cut(s) 80
PleI GAGTC 1 cut(s) 197
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 1006
PshAI GACNNNNGTC 2 cut(s) 188, 221
PsiI TTATAA 1 cut(s) 650
Psp5II RGGWCCY 1 cut(s) 1006
Psp6I CCWGG 2 cut(s) 179, 765
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 2 cut(s) 179, 765
PspN4I GGNNCC 5 cut(s) 77, 210, 253, 468, 533
PspPI GGNCC 3 cut(s) 228, 839, 1006
PspPPI RGGWCCY 1 cut(s) 1006
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 2 cut(s) 531, 1141
PvuII CAGCTG 2 cut(s) 31, 949
RsaI GTAC 4 cut(s) 151, 175, 605, 699
RsaNI GTAC 4 cut(s) 150, 174, 604, 698
RseI CAYNNNNRTG 1 cut(s) 564
SaqAI TTAA 2 cut(s) 813, 932
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 3 cut(s) 531, 597, 1141
Sau96I GGNCC 3 cut(s) 228, 839, 1006
ScaI AGTACT 1 cut(s) 605
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 3 cut(s) 181, 213, 767
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 2 cut(s) 583, 724
SfoI GGCGCC 1 cut(s) 253
SinI GGWCC 1 cut(s) 1006
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 3 cut(s) 623, 931, 1091
SmoI CTYRAG 3 cut(s) 623, 931, 1091
SpeI ACTAGT 2 cut(s) 152, 241
Sse9I AATT 4 cut(s) 202, 539, 651, 747
SseBI AGGCCT 1 cut(s) 328
SsiI CCGC 5 cut(s) 44, 79, 117, 336, 890
SspDI GGCGCC 1 cut(s) 251
SspI AATATT 1 cut(s) 1100
SspMI CTAG 4 cut(s) 153, 242, 330, 954
StuI AGGCCT 1 cut(s) 328
StyD4I CCNGG 3 cut(s) 179, 211, 765
TaaI ACNGT 5 cut(s) 373, 436, 566, 916, 1024
TaiI ACGT 1 cut(s) 312
TaqI TCGA 1 cut(s) 157
TasI AATT 4 cut(s) 202, 539, 651, 747
TatI WGTACW 2 cut(s) 149, 603
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 2 cut(s) 629, 666
Tru1I TTAA 2 cut(s) 813, 932
Tru9I TTAA 2 cut(s) 813, 932
TscAI CASTG 3 cut(s) 145, 457, 616
TseFI GTSAC 1 cut(s) 566
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 4 cut(s) 312, 419, 658, 1055
TspRI CASTG 3 cut(s) 145, 457, 616
Van91I CCANNNNNTGG 1 cut(s) 12
Vha464I CTTAAG 1 cut(s) 931
VpaK11BI GGWCC 1 cut(s) 1006
XapI RAATTY 2 cut(s) 202, 539
XceI RCATGY 1 cut(s) 663
XcmI CCANNNNNNNNNTGG 2 cut(s) 241, 262
XmiI GTMKAC 1 cut(s) 238
XmnI GAANNNNTTC 1 cut(s) 618
XspI CTAG 4 cut(s) 153, 242, 330, 954
ZrmI AGTACT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.