MD05G1131800.v1.1

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015401
Physical Location & Seq
Reverse (-)
25770917 .. 25774128
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1131800.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGGCAATGGCTTGGAGGCTTCCTACCCGGCTGCCAACACCCGGAAACCTCCATCACTACGACTCCAAGACGCTGTCGTTCCCAGCCTCACTCTCATGGTGCCGGACTTTAGCTCCGGACCATCTCCTTCCTTCGTCATCTTCTTCCACCTCCACCGCCACTGGGACCAATACGCGGCGTTTCATGAAATGTACGGCCAGGGCGGGAGCTGGGGCTGTGAATCTTGCTCCGGGGACACCCGTCAGACCCACCAGCATACTGGTGGTCGGCGCCACCGGGACTCTGGGGAGGCAGATTGTGAGGAGAGCACTTGATGAAGGCTATGATGTTAGGTGCTTGGTTAGGCCGAGACCAGCCCCTGCTGATTTCCTCAGAGATTGGGGTGCTACCGTTGTCAATGCAGACCTTAGCAAACCAGAGACCATACCTGCTACATTGGTAGGGATTCACACAATCATCGATTGTGCGACGGGCCGTCCCGAAGAGCCCATAAAAAAGGTAGATTGGGAAGGAAAAGTTGCTCTTATACAATGTGCAAAAGCAATGGGGATCCAAAAATTTGTATTCTATTCCATCCACAACTGTGACCAGCATCCTGAAGTTCCACTTATGGAGATCAAGTATTGCACTGAAAAGTTTCTCCAAGATTCAGGCATAAATCACATCATAATTCGTTTATGTGGATTCATGCAAGGGATTATTGGACAGTATGCAGTGCCTATATTAGAAGAGAAATCGGTATGGGGAACAGACGCCCCCACAAGGATTGCATACATGGACACCCAGGATGTAGCTCGACTGACCTTTATAGCTTTAAGAAATGAGAAAGTCAATGGGAAGCTTCTCACATTTGCTGGCCCTCGTGCATGGACAACCCAAGAGGTAATAACGTTGTGTGAAAGGTTTGCGGGGCAAGAGGCAAATATAACTACAGTCCCAGTTTCTGTCCTGAGAGTAACCCGTCAGCTAACTAGGCTGTTTGAGTGGACAAATGATGTTGCTGATAGATTGGCATTTTCAGAGGTTCTTTCAAGCGATACTGTTTTCTCTGTTCCAATGAGCGAAACATATGGTCTTCTTGGGGTTGACGGAAAAGATATAGTTACACTGGAGAAATATTTGCAGGACTACTTTACCAACATATTGAAGAAATTGAAAGATATCAAAGCACAATCAAAGCAATCTGATATTTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

400

Amino Acids

44.22

Weight (kDa)

8.65

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 85 - 334 3e-26 NmrA-like family
Epimerase PF01370 86 - 191 1.8e-09 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 90 - 240 1.4e-16 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015401)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35250
fragaria_vesca FvH4_2g16360 FvH4_2g16360 FvH4_2g16360
malus_domestica MD05G1131800.v1.1
prunus_persica Prupe.8G176300_v2.0.a1
pyrus_communis pycom05g12860
rosa_chinensis RchiOBHm_Chr6g0280111
rosa_laevigata RLG00000013088
rosa_multiflora Rmu_sc0007137.1_g000006
rosa_roxburghii Rroxscaffold_7G00188370 Rroxscaffold_7G00189360
rosa_rugosa Rorug06G0130000
rosa_samantha Rh6AG240300 Rh6BG244100
rosa_wichuraiana Rw6G020940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 436
AccB1I GGYRCC 2 cut(s) 99, 269
AccII CGCG 1 cut(s) 175
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 4 cut(s) 156, 175, 203, 908
AclI AACGTT 1 cut(s) 890
AclWI GGATC 2 cut(s) 544, 557
AcoI YGGCCR 1 cut(s) 195
AcsI RAATTY 1 cut(s) 557
AcuI CTGAAG 1 cut(s) 618
AcyI GRCGYC 2 cut(s) 270, 753
AfaI GTAC 1 cut(s) 193
AfiI CCNNNNNNNGG 4 cut(s) 41, 162, 174, 762
AgsI TTSAA 3 cut(s) 1032, 1147, 1156
AhdI GACNNNNNGTC 1 cut(s) 474
AjnI CCWGG 2 cut(s) 197, 783
AleI CACNNNNGTG 1 cut(s) 582
AluBI AGCT 6 cut(s) 113, 209, 794, 812, 841, 967
AluI AGCT 6 cut(s) 113, 209, 794, 812, 841, 967
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 2 cut(s) 343, 413
AlwI GGATC 2 cut(s) 544, 557
AlwNI CAGNNNCTG 2 cut(s) 359, 944
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 4 cut(s) 195, 344, 472, 856
ApeKI GCWGC 1 cut(s) 31
ApoI RAATTY 1 cut(s) 557
Asp700I GAANNNNTTC 1 cut(s) 636
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 4 cut(s) 118, 165, 472, 857
AsuC2I CCSGG 4 cut(s) 28, 42, 231, 277
AvaII GGWCC 2 cut(s) 118, 165
BamHI GGATCC 1 cut(s) 549
BanI GGYRCC 2 cut(s) 99, 269
BanII GRGCYC 1 cut(s) 489
BauI CACGAG 1 cut(s) 861
BbsI GAAGAC 1 cut(s) 1067
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 1 cut(s) 18
BccI CCATC 3 cut(s) 60, 129, 581
BceAI ACGGC 2 cut(s) 210, 459
BciT130I CCWGG 2 cut(s) 199, 785
BcnI CCSGG 4 cut(s) 28, 42, 231, 277
BcoDI GTCTC 2 cut(s) 343, 413
BfaI CTAG 1 cut(s) 972
BfmI CTRYAG 1 cut(s) 930
BfoI RGCGCY 1 cut(s) 273
BfuAI ACCTGC 1 cut(s) 436
BisI GCNGC 2 cut(s) 32, 176
BlsI GCNGC 2 cut(s) 33, 177
Bme1390I CCNGG 6 cut(s) 28, 42, 199, 231, 277, 785
Bme18I GGWCC 2 cut(s) 118, 165
BmeRI GACNNNNNGTC 1 cut(s) 474
BmgT120I GGNCC 4 cut(s) 118, 165, 472, 857
BmiI GGNNCC 4 cut(s) 101, 166, 271, 551
BmrFI CCNGG 6 cut(s) 28, 42, 199, 231, 277, 785
BmrI ACTGGG 2 cut(s) 171, 932
BmsI GCATC 1 cut(s) 601
BmuI ACTGGG 2 cut(s) 171, 932
BoxI GACNNNNGTC 1 cut(s) 239
BpiI GAAGAC 1 cut(s) 1067
BpmI CTGGAG 1 cut(s) 1130
Bpu10I CCTNAGC 1 cut(s) 407
BpuMI CCSGG 4 cut(s) 28, 42, 231, 277
Bsa29I ATCGAT 1 cut(s) 459
BsaHI GRCGYC 2 cut(s) 270, 753
BsaI GGTCTC 2 cut(s) 343, 413
BsaJI CCNNGG 3 cut(s) 198, 230, 783
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 6 cut(s) 47, 77, 97, 127, 605, 635
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 162, 174, 762
Bse1I ACTGG 4 cut(s) 166, 264, 938, 1113
Bse3DI GCAATG 2 cut(s) 12, 549
BseAI TCCGGA 1 cut(s) 115
BseBI CCWGG 2 cut(s) 199, 785
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 198, 230, 783
BseGI GGATG 3 cut(s) 573, 592, 793
BseLI CCNNNNNNNGG 4 cut(s) 41, 162, 174, 762
BseMI GCAATG 2 cut(s) 12, 549
BseMII CTCAG 2 cut(s) 385, 941
BseNI ACTGG 4 cut(s) 166, 264, 938, 1113
BseRI GAGGAG 1 cut(s) 316
BseXI GCAGC 1 cut(s) 18
BseYI CCCAGC 2 cut(s) 82, 209
Bsh1236I CGCG 1 cut(s) 175
BshFI GGCC 4 cut(s) 197, 346, 474, 858
BshNI GGYRCC 2 cut(s) 99, 269
BshVI ATCGAT 1 cut(s) 459
BsiHKAI GWGCWC 1 cut(s) 310
BsiSI CCGG 6 cut(s) 28, 42, 103, 116, 230, 276
BslFI GGGAC 5 cut(s) 178, 247, 292, 462, 920
BslI CCNNNNNNNGG 4 cut(s) 41, 162, 174, 762
BsmAI GTCTC 2 cut(s) 343, 413
BsmFI GGGAC 5 cut(s) 178, 247, 292, 462, 920
BsnI GGCC 4 cut(s) 197, 346, 474, 858
Bso31I GGTCTC 2 cut(s) 343, 413
Bsp1286I GDGCHC 2 cut(s) 310, 489
Bsp13I TCCGGA 1 cut(s) 115
Bsp143I GATC 2 cut(s) 549, 615
BspACI CCGC 4 cut(s) 156, 175, 203, 908
BspANI GGCC 4 cut(s) 197, 346, 474, 858
BspCNI CTCAG 2 cut(s) 384, 942
BspDI ATCGAT 1 cut(s) 459
BspEI TCCGGA 1 cut(s) 115
BspFNI CGCG 1 cut(s) 175
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 4 cut(s) 101, 166, 271, 551
BspMI ACCTGC 1 cut(s) 436
BspPI GGATC 2 cut(s) 544, 557
BspQI GCTCTTC 1 cut(s) 477
BspT107I GGYRCC 2 cut(s) 99, 269
BspTNI GGTCTC 2 cut(s) 343, 413
BsrDI GCAATG 2 cut(s) 12, 549
BsrI ACTGG 4 cut(s) 166, 264, 938, 1113
BssECI CCNNGG 3 cut(s) 198, 230, 783
BssMI GATC 2 cut(s) 549, 615
BssNI GRCGYC 2 cut(s) 270, 753
BssSI CACGAG 1 cut(s) 861
Bst2BI CACGAG 1 cut(s) 861
Bst2UI CCWGG 2 cut(s) 199, 785
Bst4CI ACNGT 5 cut(s) 391, 584, 708, 934, 1042
Bst6I CTCTTC 2 cut(s) 477, 723
BstACI GRCGYC 2 cut(s) 270, 753
BstC8I GCNNGC 1 cut(s) 856
BstDEI CTNAG 3 cut(s) 371, 407, 950
BstF5I GGATG 3 cut(s) 573, 592, 793
BstFNI CGCG 1 cut(s) 175
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 2 cut(s) 552, 618
BstMAI GTCTC 2 cut(s) 343, 413
BstMBI GATC 2 cut(s) 549, 615
BstMWI GCNNNNNNNGC 1 cut(s) 973
BstNI CCWGG 2 cut(s) 199, 785
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 6 cut(s) 26, 40, 197, 229, 275, 783
BstSFI CTRYAG 1 cut(s) 930
BstUI CGCG 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 1067
BstX2I RGATCY 1 cut(s) 549
BstXI CCANNNNNNTGG 1 cut(s) 259
BstYI RGATCY 1 cut(s) 549
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 4 cut(s) 197, 346, 474, 858
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 3 cut(s) 573, 592, 793
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 4 cut(s) 159, 627, 720, 1106
BveI ACCTGC 1 cut(s) 436
Cac8I GCNNGC 1 cut(s) 856
CaiI CAGNNNCTG 2 cut(s) 359, 944
CciI TCATGA 1 cut(s) 183
CfoI GCGC 1 cut(s) 272
Cfr13I GGNCC 4 cut(s) 118, 165, 472, 857
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 2 cut(s) 79, 761
Csp6I GTAC 1 cut(s) 192
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviAII CATG 5 cut(s) 96, 184, 688, 775, 867
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 3 cut(s) 371, 407, 950
DinI GGCGCC 1 cut(s) 271
DpnI GATC 2 cut(s) 551, 617
DpnII GATC 2 cut(s) 549, 615
DriI GACNNNNNGTC 1 cut(s) 474
EaeI YGGCCR 1 cut(s) 195
Eam1104I CTCTTC 2 cut(s) 477, 723
Eam1105I GACNNNNNGTC 1 cut(s) 474
EarI CTCTTC 2 cut(s) 477, 723
Eco24I GRGCYC 1 cut(s) 489
Eco31I GGTCTC 2 cut(s) 343, 413
Eco32I GATATC 1 cut(s) 1162
Eco47I GGWCC 2 cut(s) 118, 165
Eco57I CTGAAG 1 cut(s) 618
EcoRII CCWGG 2 cut(s) 197, 783
EcoRV GATATC 1 cut(s) 1162
EcoT38I GRGCYC 1 cut(s) 489
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
FaeI CATG 5 cut(s) 99, 187, 691, 778, 870
FalI AAGNNNNNCTT 4 cut(s) 507, 539, 591, 623
FaqI GGGAC 5 cut(s) 178, 247, 292, 462, 920
FatI CATG 5 cut(s) 95, 183, 687, 774, 866
FauI CCCGC 2 cut(s) 196, 901
FauNDI CATATG 1 cut(s) 1069
Fnu4HI GCNGC 2 cut(s) 32, 176
FokI GGATG 3 cut(s) 560, 579, 800
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 2 cut(s) 32, 176
FspBI CTAG 1 cut(s) 972
GlaI GCGC 1 cut(s) 271
GluI GCNGC 2 cut(s) 32, 176
GsaI CCCAGC 2 cut(s) 86, 213
GsuI CTGGAG 1 cut(s) 1130
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 4 cut(s) 197, 346, 474, 858
HapII CCGG 6 cut(s) 28, 42, 103, 116, 230, 276
HgaI GACGC 2 cut(s) 79, 761
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 2 cut(s) 270, 753
Hin1II CATG 5 cut(s) 99, 187, 691, 778, 870
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 1087
HindII GTYRAC 1 cut(s) 1087
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 6 cut(s) 62, 220, 280, 445, 647, 684
HpaII CCGG 6 cut(s) 28, 42, 103, 116, 230, 276
Hpy166II GTNNAC 2 cut(s) 987, 1087
Hpy188I TCNGA 5 cut(s) 245, 374, 1021, 1186, 1199
Hpy188III TCNNGA 5 cut(s) 116, 184, 479, 596, 949
Hpy8I GTNNAC 2 cut(s) 987, 1087
Hpy99I CGWCG 1 cut(s) 472
HpyAV CCTTC 4 cut(s) 137, 141, 311, 503
HpyCH4III ACNGT 5 cut(s) 391, 584, 708, 934, 1042
HpyCH4IV ACGT 1 cut(s) 890
HpyCH4V TGCA 8 cut(s) 401, 536, 627, 691, 713, 770, 866, 1123
HpyF10VI GCNNNNNNNGC 1 cut(s) 973
HpyF3I CTNAG 3 cut(s) 371, 407, 950
HpySE526I ACGT 1 cut(s) 890
Hsp92I GRCGYC 2 cut(s) 270, 753
Hsp92II CATG 5 cut(s) 99, 187, 691, 778, 870
HspAI GCGC 1 cut(s) 270
KasI GGCGCC 1 cut(s) 269
Kpn2I TCCGGA 1 cut(s) 115
Kzo9I GATC 2 cut(s) 549, 615
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 3 cut(s) 118, 206, 232
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 601
MaeI CTAG 1 cut(s) 972
MaeII ACGT 1 cut(s) 890
MaeIII GTNAC 3 cut(s) 584, 955, 1102
MalI GATC 2 cut(s) 551, 617
MboI GATC 2 cut(s) 549, 615
MboII GAAGA 6 cut(s) 132, 135, 494, 740, 1067, 1159
MflI RGATCY 1 cut(s) 549
MhlI GDGCHC 2 cut(s) 310, 489
MluCI AATT 3 cut(s) 557, 669, 1151
Mly113I GGCGCC 1 cut(s) 270
MlyI GAGTC 2 cut(s) 56, 274
MroI TCCGGA 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 636
MseI TTAA 1 cut(s) 815
MslI CAYNNNNRTG 3 cut(s) 94, 260, 582
MspI CCGG 6 cut(s) 28, 42, 103, 116, 230, 276
MspR9I CCNGG 6 cut(s) 28, 42, 199, 231, 277, 785
MvaI CCWGG 2 cut(s) 199, 785
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 1 cut(s) 973
NarI GGCGCC 1 cut(s) 270
NciI CCSGG 4 cut(s) 28, 42, 231, 277
NdeI CATATG 1 cut(s) 1069
NdeII GATC 2 cut(s) 549, 615
NlaIII CATG 5 cut(s) 99, 187, 691, 778, 870
NlaIV GGNNCC 4 cut(s) 101, 166, 271, 551
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 584
OliI CACNNNNGTG 1 cut(s) 582
PagI TCATGA 1 cut(s) 183
PciSI GCTCTTC 1 cut(s) 477
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 4 cut(s) 220, 445, 647, 684
PflFI GACNNNGTC 1 cut(s) 73
PkrI GCNGC 2 cut(s) 33, 177
PleI GAGTC 2 cut(s) 56, 274
PluTI GGCGCC 1 cut(s) 273
PpsI GAGTC 2 cut(s) 56, 274
PshAI GACNNNNGTC 1 cut(s) 239
Psp1406I AACGTT 1 cut(s) 890
Psp6I CCWGG 2 cut(s) 197, 783
PspFI CCCAGC 2 cut(s) 82, 209
PspGI CCWGG 2 cut(s) 197, 783
PspN4I GGNNCC 4 cut(s) 101, 166, 271, 551
PspPI GGNCC 4 cut(s) 118, 165, 472, 857
PstNI CAGNNNCTG 2 cut(s) 359, 944
PsuI RGATCY 1 cut(s) 549
PsyI GACNNNGTC 1 cut(s) 73
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
RseI CAYNNNNRTG 3 cut(s) 94, 260, 582
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 1 cut(s) 815
SatI GCNGC 2 cut(s) 32, 176
Sau3AI GATC 2 cut(s) 549, 615
Sau96I GGNCC 4 cut(s) 118, 165, 472, 857
SchI GAGTC 2 cut(s) 56, 274
ScrFI CCNGG 6 cut(s) 28, 42, 199, 231, 277, 785
SduI GDGCHC 2 cut(s) 310, 489
SfaNI GCATC 1 cut(s) 601
SfcI CTRYAG 1 cut(s) 930
SfoI GGCGCC 1 cut(s) 271
SinI GGWCC 2 cut(s) 118, 165
SmiMI CAYNNNNRTG 3 cut(s) 94, 260, 582
Sse9I AATT 3 cut(s) 557, 669, 1151
SsiI CCGC 4 cut(s) 156, 175, 203, 908
SspDI GGCGCC 1 cut(s) 269
SspI AATATT 1 cut(s) 1118
SspMI CTAG 1 cut(s) 972
StyD4I CCNGG 6 cut(s) 26, 40, 197, 229, 275, 783
TaaI ACNGT 5 cut(s) 391, 584, 708, 934, 1042
TaiI ACGT 1 cut(s) 893
TaqI TCGA 2 cut(s) 459, 796
TasI AATT 3 cut(s) 557, 669, 1151
TauI GCSGC 1 cut(s) 178
TfiI GAWTC 4 cut(s) 220, 445, 647, 684
Tru1I TTAA 1 cut(s) 815
Tru9I TTAA 1 cut(s) 815
TscAI CASTG 4 cut(s) 166, 634, 720, 1113
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 1 cut(s) 31
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 4 cut(s) 172, 200, 330, 676
TspGWI ACGGA 1 cut(s) 1104
TspRI CASTG 4 cut(s) 166, 634, 720, 1113
Tth111I GACNNNGTC 1 cut(s) 73
VpaK11BI GGWCC 2 cut(s) 118, 165
XapI RAATTY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 3 cut(s) 256, 259, 280
XmnI GAANNNNTTC 1 cut(s) 636
XspI CTAG 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.