pycom05g12860

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015401
Physical Location & Seq
Reverse (-)
16406155 .. 16424767
18613 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12860.8

Sequence Viewer

Length: 1335 bp
ATGACAAGTGGCAGAAAATCCTTATCCACTTTGGCGGACGACGTGGCAACACTGAGCTCCATCGTTCCATCTCTCCCTATCTGTAAAATGGAAAGCGTCCGTCCAGTCAGTGACAGACAAAGGGAGAGCTTGAAAATGGCAATGGCTTGGAGGCTTCCTCCCCAGCTGCCAACACCCGGAAACCTCCATCACTATGACTCCAAAACGCTGTCGTTCCCAGCCTCACTCTCATGGTGCCGGACTTTAGCTCCGGACCATCTCCTTCCTTCGTCATCTTCTTCCACCTCCACCGCCACTGGGACCAATACGCGGCGTTTCATGAAATGTACGGCCAGGGCGGGAGCTGGGGCTGTAAATCTTGCTCCGGGGACACCCGTCAGACCCACCAGCATACTGGTGGTCGGCGCCACCGGGACTCTGGGGAGGCAGATTGTGAGGAGAGCACTTGATGAAGGCTATGATGTTAGGTGCTTGGTTAGGCCGAGACCAGCCCCTGCTGATTTCCTCAGAGATTGGGGTGCTACTGTTGTCAATGCAGACCTTAGCAAACCAGAGACCATACCTGCGACATTGGTAGGGATTCACACAATCATCGACTGTGCGACGGGTCGTCCCGAAGAGCCCATAAAAAAGGTAGATTGGGAAGGAAAAGTTGCTCTTATACAATGTGCAAAAGCAATGGGGATCCAAAAATTTGTATTCTATTCCATCCACAACTGTGACCAGCATCCCGAAGTTCCACTTATGGAGATCAAGTATTGCACTGAAAAGTTTCTCCAAGATTCAGGCATAAATCACGTCATAATTCGTTTATGTGGATTCATGCAAGGGATTATTGGACAGTATGCAGTGCCTATATTAGAAGAGAAATCGGTATGGGGAACAGACGCCCCCACAAGGATTGCATACATGGACACCCAGGATGTAGCTCGCCTGACCTTTATAGCTTTAAGAAATGAGAAAGTCAATGGGAAGCTTCTCACATTTGCTGGCCCTCGCGCATGGACAACCCAAGAGGTAATAACGTTGTGTGAAAGGTTTGCGGGCCAAGAGGCAAATATAACTACAGTCCCAGTTTCTGTCCTGAGAGTAACCCGTCAGCTAACTAGGCTGTTTGAGTGGACAAATGATGTTGCTGATAGATTGGCATTTTCAGAGGTTCTTTCAAGCGATACTGTTTTCTCTGTTCCAATGAGCGAAACATATGGTCTTCTTGGGGTTGACGGAAAAGATATAGTTACACTGGAGAAATATTTGCAGGACTACTTTACCAACATATTGAAGAAATTGAAAGATATCAAAGCACAATCAAAGCAATCTGATATTTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

445

Amino Acids

49.05

Weight (kDa)

8.71

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015401)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35250
fragaria_vesca FvH4_2g16360 FvH4_2g16360 FvH4_2g16360
malus_domestica MD05G1131800.v1.1
prunus_persica Prupe.8G176300_v2.0.a1
pyrus_communis pycom05g12860
rosa_chinensis RchiOBHm_Chr6g0280111
rosa_laevigata RLG00000013088
rosa_multiflora Rmu_sc0007137.1_g000006
rosa_roxburghii Rroxscaffold_7G00188370 Rroxscaffold_7G00189360
rosa_rugosa Rorug06G0130000
rosa_samantha Rh6AG240300 Rh6BG244100
rosa_wichuraiana Rw6G020940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 571
AccB1I GGYRCC 2 cut(s) 234, 404
AccII CGCG 2 cut(s) 310, 999
AccIII TCCGGA 1 cut(s) 250
AciI CCGC 5 cut(s) 35, 291, 310, 338, 1043
AclI AACGTT 1 cut(s) 1025
AclWI GGATC 2 cut(s) 679, 692
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 1 cut(s) 692
AcyI GRCGYC 2 cut(s) 405, 888
AfaI GTAC 1 cut(s) 328
AfiI CCNNNNNNNGG 4 cut(s) 176, 297, 309, 897
AgsI TTSAA 4 cut(s) 133, 1167, 1282, 1291
AhdI GACNNNNNGTC 1 cut(s) 609
AjiI CACGTC 2 cut(s) 43, 799
AjnI CCWGG 2 cut(s) 332, 918
AleI CACNNNNGTG 1 cut(s) 717
AluBI AGCT 9 cut(s) 57, 129, 166, 248, 344, 929, 947, 976, 1102
AluI AGCT 9 cut(s) 57, 129, 166, 248, 344, 929, 947, 976, 1102
Alw21I GWGCWC 2 cut(s) 59, 445
Alw26I GTCTC 2 cut(s) 478, 548
AlwI GGATC 2 cut(s) 679, 692
AlwNI CAGNNNCTG 2 cut(s) 494, 1079
Aor13HI TCCGGA 1 cut(s) 250
AoxI GGCC 4 cut(s) 330, 479, 991, 1045
ApeKI GCWGC 1 cut(s) 166
ApoI RAATTY 1 cut(s) 692
Asp700I GAANNNNTTC 1 cut(s) 771
AspLEI GCGC 2 cut(s) 407, 1001
AspS9I GGNCC 4 cut(s) 253, 300, 992, 1045
AsuC2I CCSGG 3 cut(s) 177, 366, 412
AvaII GGWCC 2 cut(s) 253, 300
BamHI GGATCC 1 cut(s) 684
BanI GGYRCC 2 cut(s) 234, 404
BanII GRGCYC 2 cut(s) 59, 624
BbsI GAAGAC 1 cut(s) 1202
Bbv12I GWGCWC 2 cut(s) 59, 445
BbvI GCAGC 1 cut(s) 153
BccI CCATC 5 cut(s) 68, 76, 195, 264, 716
BceAI ACGGC 1 cut(s) 345
BciT130I CCWGG 2 cut(s) 334, 920
BcnI CCSGG 3 cut(s) 177, 366, 412
BcoDI GTCTC 2 cut(s) 478, 548
BfaI CTAG 1 cut(s) 1107
BfmI CTRYAG 1 cut(s) 1065
BfoI RGCGCY 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 571
BisI GCNGC 2 cut(s) 167, 311
BlsI GCNGC 2 cut(s) 168, 312
Bme1390I CCNGG 5 cut(s) 177, 334, 366, 412, 920
Bme18I GGWCC 2 cut(s) 253, 300
BmeRI GACNNNNNGTC 1 cut(s) 609
BmgBI CACGTC 2 cut(s) 43, 799
BmgT120I GGNCC 4 cut(s) 253, 300, 992, 1045
BmiI GGNNCC 4 cut(s) 236, 301, 406, 686
BmrFI CCNGG 5 cut(s) 177, 334, 366, 412, 920
BmrI ACTGGG 2 cut(s) 306, 1067
BmsI GCATC 1 cut(s) 736
BmuI ACTGGG 2 cut(s) 306, 1067
BoxI GACNNNNGTC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 1202
BplI GAGNNNNNCTC 2 cut(s) 142, 174
BpmI CTGGAG 1 cut(s) 1265
Bpu10I CCTNAGC 1 cut(s) 542
BpuMI CCSGG 3 cut(s) 177, 366, 412
BsaHI GRCGYC 2 cut(s) 405, 888
BsaI GGTCTC 2 cut(s) 478, 548
BsaJI CCNNGG 3 cut(s) 333, 365, 918
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 6 cut(s) 182, 212, 232, 262, 740, 770
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 297, 309, 897
Bse1I ACTGG 5 cut(s) 104, 301, 399, 1073, 1248
Bse3DI GCAATG 2 cut(s) 147, 684
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 2 cut(s) 334, 920
BseDI CCNNGG 3 cut(s) 333, 365, 918
BseGI GGATG 3 cut(s) 708, 727, 928
BseLI CCNNNNNNNGG 4 cut(s) 176, 297, 309, 897
BseMI GCAATG 2 cut(s) 147, 684
BseMII CTCAG 3 cut(s) 44, 520, 1076
BseNI ACTGG 5 cut(s) 104, 301, 399, 1073, 1248
BseRI GAGGAG 1 cut(s) 451
BseXI GCAGC 1 cut(s) 153
BseYI CCCAGC 3 cut(s) 162, 217, 344
Bsh1236I CGCG 2 cut(s) 310, 999
BshFI GGCC 4 cut(s) 332, 481, 993, 1047
BshNI GGYRCC 2 cut(s) 234, 404
BsiHKAI GWGCWC 2 cut(s) 59, 445
BsiSI CCGG 5 cut(s) 177, 238, 251, 365, 411
BslFI GGGAC 5 cut(s) 313, 382, 427, 597, 1055
BslI CCNNNNNNNGG 4 cut(s) 176, 297, 309, 897
BsmAI GTCTC 2 cut(s) 478, 548
BsmFI GGGAC 5 cut(s) 313, 382, 427, 597, 1055
BsnI GGCC 4 cut(s) 332, 481, 993, 1047
Bso31I GGTCTC 2 cut(s) 478, 548
Bsp1286I GDGCHC 3 cut(s) 59, 445, 624
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 2 cut(s) 684, 750
BspACI CCGC 5 cut(s) 35, 291, 310, 338, 1043
BspANI GGCC 4 cut(s) 332, 481, 993, 1047
BspCNI CTCAG 3 cut(s) 45, 519, 1077
BspEI TCCGGA 1 cut(s) 250
BspFNI CGCG 2 cut(s) 310, 999
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 4 cut(s) 236, 301, 406, 686
BspMI ACCTGC 1 cut(s) 571
BspPI GGATC 2 cut(s) 679, 692
BspQI GCTCTTC 1 cut(s) 612
BspT107I GGYRCC 2 cut(s) 234, 404
BspTNI GGTCTC 2 cut(s) 478, 548
BsrDI GCAATG 2 cut(s) 147, 684
BsrI ACTGG 5 cut(s) 104, 301, 399, 1073, 1248
BssECI CCNNGG 3 cut(s) 333, 365, 918
BssMI GATC 2 cut(s) 684, 750
BssNI GRCGYC 2 cut(s) 405, 888
Bst2UI CCWGG 2 cut(s) 334, 920
Bst4CI ACNGT 6 cut(s) 526, 599, 719, 843, 1069, 1177
Bst6I CTCTTC 2 cut(s) 612, 858
BstACI GRCGYC 2 cut(s) 405, 888
BstC8I GCNNGC 3 cut(s) 931, 991, 1045
BstDEI CTNAG 4 cut(s) 53, 506, 542, 1085
BstF5I GGATG 3 cut(s) 708, 727, 928
BstFNI CGCG 2 cut(s) 310, 999
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 2 cut(s) 407, 1001
BstKTI GATC 2 cut(s) 687, 753
BstMAI GTCTC 2 cut(s) 478, 548
BstMBI GATC 2 cut(s) 684, 750
BstMWI GCNNNNNNNGC 1 cut(s) 1108
BstNI CCWGG 2 cut(s) 334, 920
BstPAI GACNNNNGTC 1 cut(s) 374
BstSCI CCNGG 5 cut(s) 175, 332, 364, 410, 918
BstSFI CTRYAG 1 cut(s) 1065
BstUI CGCG 2 cut(s) 310, 999
BstV1I GCAGC 1 cut(s) 153
BstV2I GAAGAC 1 cut(s) 1202
BstX2I RGATCY 1 cut(s) 684
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 1 cut(s) 684
BsuRI GGCC 4 cut(s) 332, 481, 993, 1047
BtrI CACGTC 2 cut(s) 43, 799
BtsCI GGATG 3 cut(s) 708, 727, 928
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 6 cut(s) 50, 115, 294, 762, 855, 1241
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 3 cut(s) 931, 991, 1045
CaiI CAGNNNCTG 2 cut(s) 494, 1079
CciI TCATGA 1 cut(s) 318
CfoI GCGC 2 cut(s) 407, 1001
Cfr13I GGNCC 4 cut(s) 253, 300, 992, 1045
CseI GACGC 2 cut(s) 85, 896
Csp6I GTAC 1 cut(s) 327
CspCI CAANNNNNGTGG 2 cut(s) 883, 918
CviAII CATG 5 cut(s) 231, 319, 823, 910, 1002
CviQI GTAC 1 cut(s) 327
DdeI CTNAG 4 cut(s) 53, 506, 542, 1085
DinI GGCGCC 1 cut(s) 406
DpnI GATC 2 cut(s) 686, 752
DpnII GATC 2 cut(s) 684, 750
DriI GACNNNNNGTC 1 cut(s) 609
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 2 cut(s) 612, 858
Eam1105I GACNNNNNGTC 1 cut(s) 609
EarI CTCTTC 2 cut(s) 612, 858
EciI GGCGGA 1 cut(s) 50
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 2 cut(s) 59, 624
Eco31I GGTCTC 2 cut(s) 478, 548
Eco32I GATATC 1 cut(s) 1297
Eco47I GGWCC 2 cut(s) 253, 300
Eco53kI GAGCTC 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 57
EcoRII CCWGG 2 cut(s) 332, 918
EcoRV GATATC 1 cut(s) 1297
EcoT38I GRGCYC 2 cut(s) 59, 624
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
FaeI CATG 5 cut(s) 234, 322, 826, 913, 1005
FalI AAGNNNNNCTT 4 cut(s) 642, 674, 726, 758
FaqI GGGAC 5 cut(s) 313, 382, 427, 597, 1055
FatI CATG 5 cut(s) 230, 318, 822, 909, 1001
FauI CCCGC 2 cut(s) 331, 1036
FauNDI CATATG 1 cut(s) 1204
Fnu4HI GCNGC 2 cut(s) 167, 311
FokI GGATG 3 cut(s) 695, 714, 935
FriOI GRGCYC 2 cut(s) 59, 624
Fsp4HI GCNGC 2 cut(s) 167, 311
FspBI CTAG 1 cut(s) 1107
GlaI GCGC 2 cut(s) 406, 1000
GluI GCNGC 2 cut(s) 167, 311
GsaI CCCAGC 3 cut(s) 166, 221, 348
GsuI CTGGAG 1 cut(s) 1265
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 4 cut(s) 332, 481, 993, 1047
HapII CCGG 5 cut(s) 177, 238, 251, 365, 411
HgaI GACGC 2 cut(s) 85, 896
HhaI GCGC 2 cut(s) 407, 1001
Hin1I GRCGYC 2 cut(s) 405, 888
Hin1II CATG 5 cut(s) 234, 322, 826, 913, 1005
Hin6I GCGC 2 cut(s) 405, 999
HinP1I GCGC 2 cut(s) 405, 999
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HindIII AAGCTT 1 cut(s) 974
HinfI GANTC 5 cut(s) 197, 415, 580, 782, 819
HpaII CCGG 5 cut(s) 177, 238, 251, 365, 411
Hpy166II GTNNAC 2 cut(s) 1122, 1222
Hpy188I TCNGA 5 cut(s) 380, 509, 1156, 1321, 1334
Hpy188III TCNNGA 5 cut(s) 251, 319, 614, 731, 1084
Hpy8I GTNNAC 2 cut(s) 1122, 1222
Hpy99I CGWCG 2 cut(s) 44, 607
HpyAV CCTTC 4 cut(s) 272, 276, 446, 638
HpyCH4III ACNGT 6 cut(s) 526, 599, 719, 843, 1069, 1177
HpyCH4IV ACGT 3 cut(s) 42, 798, 1025
HpyCH4V TGCA 7 cut(s) 536, 671, 762, 826, 848, 905, 1258
HpyF10VI GCNNNNNNNGC 1 cut(s) 1108
HpyF3I CTNAG 4 cut(s) 53, 506, 542, 1085
HpySE526I ACGT 3 cut(s) 42, 798, 1025
Hsp92I GRCGYC 2 cut(s) 405, 888
Hsp92II CATG 5 cut(s) 234, 322, 826, 913, 1005
HspAI GCGC 2 cut(s) 405, 999
KasI GGCGCC 1 cut(s) 404
Kpn2I TCCGGA 1 cut(s) 250
Kzo9I GATC 2 cut(s) 684, 750
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 4 cut(s) 62, 253, 341, 367
Lsp1109I GCAGC 1 cut(s) 153
LweI GCATC 1 cut(s) 736
MaeI CTAG 1 cut(s) 1107
MaeII ACGT 3 cut(s) 42, 798, 1025
MaeIII GTNAC 4 cut(s) 110, 719, 1090, 1237
MalI GATC 2 cut(s) 686, 752
MboI GATC 2 cut(s) 684, 750
MboII GAAGA 6 cut(s) 267, 270, 629, 875, 1202, 1294
MflI RGATCY 1 cut(s) 684
MhlI GDGCHC 3 cut(s) 59, 445, 624
MluCI AATT 3 cut(s) 692, 804, 1286
Mly113I GGCGCC 1 cut(s) 405
MlyI GAGTC 2 cut(s) 191, 409
MroI TCCGGA 1 cut(s) 250
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 1 cut(s) 950
MslI CAYNNNNRTG 4 cut(s) 192, 229, 395, 717
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 5 cut(s) 177, 238, 251, 365, 411
MspR9I CCNGG 5 cut(s) 177, 334, 366, 412, 920
MvaI CCWGG 2 cut(s) 334, 920
MvnI CGCG 2 cut(s) 310, 999
MwoI GCNNNNNNNGC 1 cut(s) 1108
NarI GGCGCC 1 cut(s) 405
NciI CCSGG 3 cut(s) 177, 366, 412
NdeI CATATG 1 cut(s) 1204
NdeII GATC 2 cut(s) 684, 750
NlaIII CATG 5 cut(s) 234, 322, 826, 913, 1005
NlaIV GGNNCC 4 cut(s) 236, 301, 406, 686
NmeAIII GCCGAG 1 cut(s) 507
NmuCI GTSAC 2 cut(s) 110, 719
OliI CACNNNNGTG 1 cut(s) 717
PagI TCATGA 1 cut(s) 318
PciSI GCTCTTC 1 cut(s) 612
PdmI GAANNNNTTC 1 cut(s) 771
PfeI GAWTC 3 cut(s) 580, 782, 819
PkrI GCNGC 2 cut(s) 168, 312
PleI GAGTC 2 cut(s) 191, 409
PluTI GGCGCC 1 cut(s) 408
PpsI GAGTC 2 cut(s) 191, 409
PshAI GACNNNNGTC 1 cut(s) 374
Psp124BI GAGCTC 1 cut(s) 59
Psp1406I AACGTT 1 cut(s) 1025
Psp6I CCWGG 2 cut(s) 332, 918
PspFI CCCAGC 3 cut(s) 162, 217, 344
PspGI CCWGG 2 cut(s) 332, 918
PspN4I GGNNCC 4 cut(s) 236, 301, 406, 686
PspPI GGNCC 4 cut(s) 253, 300, 992, 1045
PstNI CAGNNNCTG 2 cut(s) 494, 1079
PsuI RGATCY 1 cut(s) 684
PvuII CAGCTG 1 cut(s) 166
RsaI GTAC 1 cut(s) 328
RsaNI GTAC 1 cut(s) 327
RseI CAYNNNNRTG 4 cut(s) 192, 229, 395, 717
SacI GAGCTC 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 1 cut(s) 950
SatI GCNGC 2 cut(s) 167, 311
Sau3AI GATC 2 cut(s) 684, 750
Sau96I GGNCC 4 cut(s) 253, 300, 992, 1045
SchI GAGTC 2 cut(s) 191, 409
ScrFI CCNGG 5 cut(s) 177, 334, 366, 412, 920
SduI GDGCHC 3 cut(s) 59, 445, 624
SfaNI GCATC 1 cut(s) 736
SfcI CTRYAG 1 cut(s) 1065
SfoI GGCGCC 1 cut(s) 406
SinI GGWCC 2 cut(s) 253, 300
SmiMI CAYNNNNRTG 4 cut(s) 192, 229, 395, 717
Sse9I AATT 3 cut(s) 692, 804, 1286
SsiI CCGC 5 cut(s) 35, 291, 310, 338, 1043
SspDI GGCGCC 1 cut(s) 404
SspI AATATT 1 cut(s) 1253
SspMI CTAG 1 cut(s) 1107
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 5 cut(s) 175, 332, 364, 410, 918
TaaI ACNGT 6 cut(s) 526, 599, 719, 843, 1069, 1177
TaiI ACGT 3 cut(s) 45, 801, 1028
TaqI TCGA 1 cut(s) 594
TasI AATT 3 cut(s) 692, 804, 1286
TauI GCSGC 1 cut(s) 313
TfiI GAWTC 3 cut(s) 580, 782, 819
Tru1I TTAA 1 cut(s) 950
Tru9I TTAA 1 cut(s) 950
TscAI CASTG 6 cut(s) 57, 115, 301, 769, 855, 1248
TseFI GTSAC 2 cut(s) 110, 719
TseI GCWGC 1 cut(s) 166
Tsp45I GTSAC 2 cut(s) 110, 719
TspDTI ATGAA 4 cut(s) 307, 335, 465, 811
TspGWI ACGGA 2 cut(s) 89, 1239
TspRI CASTG 6 cut(s) 57, 115, 301, 769, 855, 1248
VpaK11BI GGWCC 2 cut(s) 253, 300
XapI RAATTY 1 cut(s) 692
XcmI CCANNNNNNNNNTGG 3 cut(s) 391, 394, 415
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.